Optika M-1120.N Booklet

OPTIKA

MICROSCOPES

®

ITALY

Rev.30.2

Objectives strongly determine the performance of your microscope!

OPTIKA OBJECTIVES

Find the most suitable lenses for your application.

Parfocal Distance

(complies with ISO standard)

OBJECTIVE FEATURES

Mounting Thread

(complies with ISO standard)

Objective Type

Magnication

Optical System

Magnication Indication

Color Band

(complies with Cover Glass ISO

standard)

Cover Glass

Slide Glass

PLAN

100x / 1.25

Oil/Water

∞/0.17

Numerical Aperture (N.A.)

Immersion Media

Cover Glass Thickness

Working

Distance (W.D.)

Anti-fungus treatment

OBSERVATION METHODS

BF PO

DF

PH

Brightfield Polarized Light

Darkfield

Phase Contrast

RPC

DIC

Relief Phase Contrast

Differential Interference Contrast

FL

FL UV

Fluorescence

UV Fluorescence

OPTICAL TERMINOLOGY

1. FN and Practical Field of View

The eld number (FN) is the size (in mm) of the eyepiece diaphragm, which denes the viewable area of a specimen. The diameter on the sample plane that can actually be viewed through the eyepiece is known as the practical eld of view (FOV) and is determined by the following formula:

FOV = (mm)

Example:

B-510BF (F.N. 22) with 10x IOS W-PLAN objective

22 FOV = = 2.2 (mm) 10

2. Working Distance

Eyepiece FN

Objective Magnication

5. Resolving Power

The lateral resolution of a microscope is the minimum distance between two points, which still makes it possible to distinguish them. The resolving power (ε) is the reciprocal of the lateral resolution. The larger the NA, the higher the resolving power.

The following formula is commonly used for the calculation of the resolution.

ε = 0.61 * (Raleigh’s formula)

λ

N.A.

λ: Wavelength of radiation in use (λ=0.55 μm for visible light.)

NA: Objective NA

Example:

IOS W-PLAN 100X (NA=1.25), λ=0.55 μm

ε = 0.61 * = 0.61 * = = 0.2684 μm

λ

N.A.

0.55

1.25

0.3355

1.25

The working distance (W.D.) is the distance between the objective front lens and the specimen surface (or the surface of the cover glass when using a cover glass objective) when the specimen is in focus.

Objectives can be divided into “normal” WD and “LWD (Long Working Distance)”. LWD objectives are typically used in inverted microscopes. Sometimes LWD are also used in upright metallurgical microscopes.

3. Parfocal Distance

The parfocal distance is the distance between the objective mounting plane and the specimen. In OPTIKA objectives, the parfocal distance is typically designed to be 45 mm.

4. Numerical Aperture (NA)

The numerical aperture is the main factor for the performance of an objective (resolving power, focal depth, and brightness). The NA is determined by the following formula:

6. Aberrations

The main aberrations (defects of the system in forming a

sharp and resolved image), which aict the microscopes,

and their possible corrections can be summarized as follows:

• Geometric

• Chromatic

While we can neglect some aberrations like Astigmatic or Coma, the main Geometric aberrations are:

(1) Field Curvature The image of a plane object, that is the set of radiations coming from the points that form an extended object perpendicular to the optical axis, is formed on a curved surface. The aberrant arrangement on the image plane is then referred to as eld curvature. Therefore, when you focus perfectly on the central part of the image, blurring occurs in the peripheral areas of the image. To have the entire image in focus, including the periphery, you need to correctly compensate for this type of aberration.

NA= n × sinθ

n: the refractive index of the medium where the lens

operates. (Air: n=1, oil: n=1.515)

θ: the angular aperture of the lens, that is the half angle

of the light cone entering in the objective.

(2) Distortion When there is no exact correspondence between the real object and its representation on the image plane, this aberration is called “distortion”. When distortion is present, a square image appears in the shape of a barrel or pincushion. This aberration aects only the shape and is due to the features of the optical system in use, which having a certain

3

physical thickness (even variable) moves away from the theory of the thin lens.

Physically, the eect is due to the dierent magnication

power of the various parts of the optical system, which generally varies radially with respect to the optical axis.

(3) Chromatic Aberration Chromatic aberrations are the types of optical aberration that occur in refractive optical systems with light formed by a set of electromagnetic radiations of dierent wavelengths. The phenomenon of refraction deviates the path of the light of an angle that also varies according to the wavelength of the radiation. Just like a prism breaks down the white light into its components, so even a converging lens will have dierent points of focus depending on the wavelength of the incident light and will create an image with undesirable coloured halos.

7. Objectives denomination

(1) Achromatic The most common objectives used in laboratory microscopes are Achromatic objectives, for which perfect coincidence is obtained for only two colours of the spectrum (red and blue). Both are brought to a single common focal point.

(2) PLAN Achromatic These objectives have the same corrections of chromatic aberrations as an Achromatic objective.

PLAN Achromatic objectives provide atness corrections

with respect to achromatic objectives. It is important to specify that an PLAN Achromatic objective must inform (according to ISO standard) on which FOV is planar.

(3) PLAN Fluorite (SemiAphochromatic) Fluorite objectives are derived from advanced glass for-

mulations containing materials such as uorite or newer synthetic substitutes that signicantly improve optical

aberration correction. Like Achromatic, Fluorite objectives are chromatically corrected for red and blue light, but are also spherically corrected for two or three colours instead of a single colour, such as Achromatic. Fluorite objectives have a higher numerical aperture, which translates into brighter images and better resolving power.

(4) PLAN APO (Apochromatic) Apochromatic objectives have the highest level of correction. Apochromatics almost completely eliminate chromatic aberration, are usually chromatically corrected for three colours (red, green and blue) and are spherically corrected for two or three wavelengths. Due to their high level of correction, apochromatic lenses usually have higher numerical apertures for a given ma-

gnication than Achromatic or Fluorite objectives.

8. Observation Modes

(1) Brighteld

Brighteld observation does not require any special accessories. The light generated by the light source is optimized by the condenser, passes through the sample and then collected by the objective that forms an image.

(2) Darkeld Oblique illumination not direct on the sample. The method uses the phenomenon of diraction and refraction of the light produced by the contours and the structure of the object. .

(3) Phase Contrast

Invented in 1932 by Zernicke, it exploits the dierences

in refractive index and thickness between the sample and the surrounding area. It allows the observation of samples not stained (or not stainable) and of extremely small thickness, that in bright-

eld cannot be observed.

(4) Relief Phase Contrast Modulation phase contrast is a new modication of conventional phase contrast which leads to visible improvements of image quality in light microscopy. In particular: contrast, focal depth, sharpness, three dimensionality, planeness, and halo artifacts can be improved.

(5) Polarized light This technique is based on the properties of some anisotropic or birefringent substances (minerals, vitamins, chlorophyll, collagen, etc.). It is used in specic areas of microscopic analysis, in particular mineralogy, in which case it is possible to collect unique and subjective data for the recognition of the mineral (thanks to the use of polarized light), but also in some applications in biology or medicine (gout analysis, study of cell membranes).

(6) DIC - Dierential Interference Contrast

Like phase contrast, this method is used to observe tran-

sparent structures not otherwise visible in brighteld. It combines interference and polarization eects and pro-

vides more contrasted images with a three-dimensional

eect.

(7) Fluorescence Certain substances, when reached by light with high energy and low wavelength, have the property to emit radiation with a wavelength greater than the exciting radiation. The substances that behave in this way are said to have a

primary uorescence.

In substances that do not have this characteristic it is pos-

sible to induce a secondary uorescence by colouring them with uorescent substances called uorochromes,

which are organic compounds capable of reacting chemically with the substrate without altering and have an

observable uorescence in the specic localization sites

even at a minimum concentration.

4

Table of contents

Achromatic Objectives

Plan Objectives

ACH Series p. 4

HC Series

N-PLAN Series p. 6

IOS N-PLAN Series

IOS N-PLAN POL Series

W-PLAN Series

IOS W-PLAN Series

W-PLAN PH Series

IOS W-PLAN PH Series

IOS W-PLAN MET Series

IOS W-PLAN POL Series

IOS LWD W-PLAN POL Series

IOS LWD W-PLAN Series

Semi-APO Objectives

Plan-APO Objectives

IOS LWD W-PLAN PH Series

IOS LWD U-PLAN RPC Series

IOS LWD U-PLAN POL Series

IOS LWD U-PLAN MET Series

IOS LWD U-PLAN MET BD Series

IOS W-PLAN F Series p. 16

IOS U-PLAN F Series

IOS LWD U-PLAN F Series

IOS LWD U-PLAN F PH Series

IOS U-PLAN F PH Series

IOS LWD U-PLAN F MET Series

IOS LWD U-PLAN F MET BD Series

IOS U-PLAN APO Series p. 19

Objective Benchmark Table

p. 20

5

Achromatic Objectives - ACH Series

ACH

160

ACH objectives are designed for B-60, M-100FX & M-100FLed.

160

These cost-eective standard Achromatic objectives for transmitted light brighteld observation are best-suited to routine work as well as educational and training purposes.

NUMERICAL

APERTURE

BF

18

CODE MAG.

M-131 4x 0.10 18 18 160 0.17

M-132 10x 0.25 7 18 160 0.17

M-133 20x 0.40 2 18 160 0.17

M-134 40x 0.65 0.53 18 160 0.17 O

M-135 60x 0.80 0.13 18 160 0.17 O

M-136 100x 1.25 0.13 18 160 0.17 OIL O

Achromatic Objectives - HC Series

HC

160

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

HC Achromatic objectives ensure versatile and reasonably priced entry-level lenses for brighteld, darkeld and simple polarization applications.

They are specically designed to achieve optimal contrast and thus maximize yield on an instrument intended for education on F.N. 18.

100x/1.25 (oil) can operate using water instead of oil for training purposes.

HC objectives are designed for B-150.

160

18

BF

PO

6

DF

CODE MAG.

M-137 4x 0.10 18 18 160 0.17

M-138 10x 0.25 7 18 160 0.17

M-139 20x 0.40 2 18 160 0.17

M-141 40x 0.65 0.53 18 160 0.17 O

M-142 60x 0.80 0.45 18 160 0.17 O

M-143 100x 1.25 0.13 18 160 0.17 OIL /WATER O

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

Plan Objectives - N-PLAN Series

N-PLAN (Plan Achromatic) objectives stand out for their quality/price ratio, providing a recommendable soultion especially in the educational eld and for routine laboratory applications.

N-PLAN

160

N-PLAN objectives are designed for B-150PL, B-190PL, B-290 & B-380

Series, with nite optical system.

160

20

DFBF

CODE MAG.

M-164 4x 0.10 15.2 20 160 0.17

M-165 10x 0.25 5.5 20 160 0.17

M-166 20x 0.40 3.5 20 160 0.17

M-167 40x 0.65 0.45 20 160 0.17 O

M-168 60x 0.85 0.45 20 160 0.17 O

M-169 100x 1.25 0.13 20 160 0.17 OIL /WATER O

M-059 100x 1.25 0.13 22 160 0.17 OIL O IRIS

NUMERICAL

APERTURE

W.D.

(mm)

Designed to ensure eld atness up to F.N. 20, with 160mm tube length.

100x/1.25 (oil) can operate using water instead of oil for training purposes.

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

Plan Objectives - IOS N-PLAN Series

N-PLAN

∞

IOS N-PLAN objectives are designed for B-290 & B-380 Series, with

innity-corrected optical system.

IOS

FL

20

DFBF

CODE MAG.

M-144 4x 0.10 16.8 20 ∞ 0.17

M-145 10x 0.25 5.8 20 ∞ 0.17

M-146 20x 0.40 5.1 20 ∞ 0.17

M-147 40x 0.65 0.43 20 ∞ 0.17 O

M-149 60x 0.80 0.14 20 ∞ 0.17 O

M-148 100x 1.25 0.13 20 ∞ 0.17 OIL /WATER O

NUMERICAL

APERTURE

IOS N-PLAN (Plan Achromatic) objectives stand out for their quality/price ratio, providing a recommendable soultion especially in the educational eld and for routine laboratory applications.

Designed to ensure eld atness up to F.N. 20, based on innity-corrected optical system.

100x/1.25 (oil) can operate using water instead of oil for training purposes.

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

7

Plan Objectives - IOS N-PLAN POL Series

IOS N-PLAN POL (Plan Achromatic) objectives stand out for their exibility in dierent techniques and quality/price ratio, providing a recommendable

N-PLAN

POL

solution with a dedicated design not to aect the polarized light, hence ensuring good contrast and measurement precision. Extensively used in education eld and for laboratory routine applica tions.

∞

IOS N-PLAN POL objectives are designed for B-383POL.

IOS

20

DFBF

FLPO

CODE MAG.

M-144P 4x 0.10 16.8 20 ∞ 0.17

M-145P 10x 0.25 5.8 20 ∞ 0.17

M-146P 20x 0.40 5.1 20 ∞ 0.17

M-147P 40x 0.65 0.43 20 ∞ 0.17 O

M-149P 60x 0.80 0.14 20 ∞ 0.17 O

M-148P 100x 1.25 0.13 20 ∞ 0.17 OIL /WATER O

NUMERICAL

APERTURE

These strain-free objectives are designed to ensure eld atness up to F.N. 20, based on innity-corrected optical system.

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

Plan Objectives - IOS W-PLAN Series

W-PLAN

∞

IOS W-PLAN objectives are designed for B-510 Series and upright modular systems.

IOS

FL

22

DFBF

CODE MAG.

M-1049 2x 0.08 19.4 22 ∞ 0.17 *

M-1125 4x 0.10 17.3 22 ∞ 0.17

M-1126 10x 0.25 10 22 ∞ 0.17

M-1127 20x 0.40 5.1 22 ∞ 0.17

M-1128 40x 0.65 0.54 22 ∞ 0.17 O

M-634.1 50x 0.95 0.19 22 ∞ 0.17 OIL O

M-1129 60x 0.80 0.14 22 ∞ 0.17 O

M-1130.1 100x 0.36 - 1.25 0.18 22 ∞ 0.17 OIL O IRIS

M-1130 100x 1.25 0.13 22 ∞ 0.17 OIL O

* Additional lens needed when using 2x on B-510 Series

NUMERICAL

APERTURE

IOS W-PLAN (Plan Achromatic) objectives represent the best cost-effective choice for high contrast and resolution, matching all the requirements of labs requiring routinary optics.

They are designed to ensure eld atness up to F.N. 22, based on innity-corrected optical system.

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

8

Plan Objectives - W-PLAN PH Series

W-PLAN

PH

160

W-PLAN PH objectives are designed for B-380, with phase contrast and innite optical system.

160

W-PLAN PH (Plan Achromatic) objectives deliver outstanding performance in phase contrast technique, providing a great contrast generally required in high-level education and routine laboratory needs.

These phase contrast objectives are designed to ensure eld at ness up to F.N. 22, with 160mm tube length.

PH

22

DFBF

PO

CODE MAG.

M-170 10x 0.25 12.2 22 160 0.17

M-171 20x 0.40 5 22 160 0.17

M-172 40x 0.65 0.37 22 160 0.17 O

M-182 100x 1.25 0.13 22 160 0.17 OIL O

NUMERICAL

APERTURE

W.D. (mm)

Plan Objectives - IOS W-PLAN PH Series

IOS W-PLAN (Plan Achromatic) PH objectives deliver outstanding performan ce in phase contrast technique, providing a great contrast ge-

W-PLAN

PH

∞

nerally required in high-level education and routine laboratory needs.

These phase contrast objectives are designed to ensure eld at ness up to F.N. 22, based on innity-corrected optical system.

F.N.

OPTICAL SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

IOS W-PLAN PH objectives are designed for B-380, B-510 and upright modular systems with phase contrast, and innity-corrected optical system.

IOS

NUMERICAL

APERTURE

PH

22

DFBF

CODE MAG.

M-1120.N 10x 0.25 10 22 ∞ 0.17

M-1121.N 20x 0.40 5.1 22 ∞ 0.17

M-1122.N 40x 0.65 0.54 22 ∞ 0.17 O

M-1123.N 100x 1.25 0.13 22 ∞ 0.17 OIL O

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

9

Plan Objectives - IOS W-PLAN MET Series

IOS W-PLAN (Plan Achromatic) MET objectives deliver precise performance without the need of the cover slide, being NCG (no cover glass).

W-PLAN

They are intended to be used in metallurgical and epi-illumination applications especially, being addressed for routine laboratory needs.

MET

∞

IOS W-PLAN MET objectives are designed for B-380 and B-510 used in

metallurgical applications.

IOS LWD W-PLAN MET 2.5x objective includes a depolarizer plate and delivers precise performance without the need of the cover slide, being NCG (no cover glass). It is the ideal solution to reach optimal contrast for epi-illumination with low-magnication. Long Working Distance provides a wider working space between the objective front lens and the specimen, a benet for a variety of samples.

They are designed to ensure eld atness up to F.N. 22, based on inni ty-corrected optical system.

IOS

BF

PO

22

CODE MAG.

M-1099 2.5x 0.08 11.3 22 ∞ -

M-336 5x 0.12 15.5 22 ∞ -

M-338 10x 0.25 10 22 ∞ -

M-339 20x 0.40 5.8 22 ∞ -

M-335 50X 0.75 0.32 22 ∞ - O

M-698.2 100x 0.80 3.2 22 ∞ -

NUMERICAL

APERTURE

W.D.

(mm)

Plan Objectives - IOS U-PLAN POL Series

IOS U-PLAN (Plan Achromatic) POL objectives stand out for their exibility in dierent techniques and deliver top-class performance when used with

U-PLAN

POL

∞

polarized light. The specic design makes them perfect for polarized light, driving to a formidable contrast and measurement precision, ideal for routine analysis in material science.

These strain-free objectives ensure eld atness up to F.N. 22, based on innity-corrected optical system.

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

DEPOLA-

RIZER

IOS U-PLAN POL objectives are designed for upright modular systems used in polarized light applications.

IOS

NUMERICAL

APERTURE

10

22

DFBF

FLPO

CODE MAG.

M-1080 4x 0.10 20.8 22 ∞ 0.17

M-1081 10x 0.25 5.3 22 ∞ 0.17

M-1081.5 20x 0.45 1.56 22 ∞ 0.17

M-1082 40x 0.65 0.36 22 ∞ 0.17 O

M-1083 60x 0.85 0.30 22 ∞ 0.17 O

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

Plan Objectives - IOS W-PLAN POL Series

IOS W-PLAN (Plan Achromatic) POL objectives stand out for their exibility in dierent techniques and deliver top-class performance when used with

W-PLAN

POL

polarized light. The specic design makes them perfect for polarized light, driving to a formidable contrast and measurement precision, ideal for routine analysis in material science.

∞

IOS W-PLAN POL objectives are designed for B-510POL used in polarized light applications.

IOS

22

DFBF

FLPO

CODE MAG.

M-1131 4x 0.10 17.3 22 ∞ 0.17

M-1132 10x 0.25 10.0 22 ∞ 0.17

M-1133 20x 0.45 0.40 22 ∞ 0.17

M-1134 40x 0.65 0.54 22 ∞ 0.17 O

M-1135 60x 0.80 0.14 22 ∞ 0.17 O

NUMERICAL

APERTURE

These strain-free objectives ensure eld atness up to F.N. 22, based on innity-corrected optical system.

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

Plan Objectives - IOS LWD W-PLAN POL Series

IOS LWD W-PLAN (Plan Achromatic) POL objectives stand out for their exibility in dierent techniques and deliver top-class performance when

LWD

W-PLAN

POL

∞

IOS LWD W-PLAN POL objectives are designed for B-510POL-I used in

polarized light applications.

used with polarized light. The specic design makes them perfect for polarized light, driving to a formidable contrast and measurement precision, ideal for routine analysis in material science/analysis. Long Working Distance provides a wider working space between the objective front lens and the specimen, a benet for a variety of samples. They deliver precise performance without the need of the cover slide, being NCG (no cover glass), specic for epi-illumination.

These strain-free objectives ensure eld atness up to F.N. 22, based on innity-corrected optical system.

IOS

22

POBF

CODE MAG.

M-1136 5x 0.12 15.5 22 ∞ -

M-1137 10x 0.25 10.0 22 ∞ -

M-1138 20x 0.40 5.8 22 ∞ -

M-1139 50x 0.75 0.32 22 ∞ -

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

FL

11

Plan Objectives - IOS LWD W-PLAN Series

iOS LWD W-PLAN (Plan Achromatic) objectives are designed for inverted micro scopes to ensure high resolution and contrast for various

LWD

W-PLAN

∞

IOS LWD W-PLAN objectives are designed for IM-3 and inverted modular systems.

applications, especially clinical examinations and cell testing, and matching all the requi rements of labs requiring routinary optics. Long Working Distance provides a wider working space between the objective front lens and the specimen, a benet for a variety of samples.

They are designed to ensure eld atness up to F.N. 22, based on inni ty-corrected optical system.

IOS

22

BF FL

CODE MAG.

M-782 10x 0.13 10.4 22 ∞ 1.2

M-773 40x 0.60 3.10 22 ∞ 1.2

M-786 60x 0.70 1.70 22 ∞ 1.2

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

Plan Objectives - IOS LWD W-PLAN PH Series

IOS LWD W-PLAN (Plan Achromatic) PH objectives are designed for inverted micro scopes to deliver outstanding performance in (positive) phase

LWD

W-PLAN

PH

∞

contrast technique, providing high resolution and contrast for observation of culture speci mens, clinical examinations and cell testing. Long Working Distance provides a wider working space between the objective front lens and the specimen, a benet for a variety of samples.

These phase contrast objectives are designed to ensure eld atness up to F.N. 22, based on innity-corrected optical system.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

IOS LWD W-PLAN PH objectives are designed for IM-3 and inverted

modular systems.

IOS

NUMERICAL

APERTURE

12

BF

FL

22

PH

CODE MAG.

M-782.1 4x 0.13 10.4 22 ∞ 1.2

M-783N 10x 0.25 7.3 22 ∞ 1.2

M-784N 20x 0.40 6.8 22 ∞ 1.2

M-785 40x 0.65 3.00 22 ∞ 1.2

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

Plan Objectives - IOS LWD U-PLAN RPC Series

IOS LWD U-PLAN RPC (Relief Phase Contrast) objectives are specially designed to provide sharp images in relief phase contrast technique, with a

U-PLAN

RPC

∞

IOS LWD U-PLAN RPC objective is designed for inverted microscopes used in biological applications.

great contrast and a superb three-dimensional eect required in high-level routine laboratory .

They are designed to ensure eld atness up to F.N. 22, based on innity-cor rected optical system.

IOS

22

RPCBF

CODE MAG.

M-861 4X 0.13 10.75 22 ∞ 1.2 *

M-862 10x 0.25 7.45 22 ∞ 1.2 **

M-863 20x 0.40 6.92 22 ∞ 1.2 **

M-864 40x 0.65 2.74 22 ∞ 1.2 **

* The use of the slider M-860.1 is required. ** The use of the slider M-860 is required.

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

13

Plan Objectives - IOS LWD U-PLAN POL Series

IOS LWD U-PLAN (Plan Achromatic) POL objectives represent the stateof-the-art lenses for upright microscopes, specically delivering the greatest

LWD

U-PLAN

POL

∞

IOS LWD U-PLAN POL objectives are designed for upright modular systems used in polarized light applications.

performance when used with polarized light without the need of the cover slide, being NCG (no cover glass). The specic design makes them perfect for polarized light, driving to an excellent, ultra-eective contrast and measurement precision. Long Working Distance provides a wider working space between the objective front lens and the specimen, a benet for a variety of samples.

These strain-free objectives ensure eld atness up to F.N. 25.

IOS

25

POBF

CODE MAG.

M-1090 5x 0.15 10.8 25 ∞ -

M-1091 10x 0.30 10 25 ∞ -

M-1092 20x 0.45 4 25 ∞ -

M-1093 50x 0.55 7.9 25 ∞ -

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

FL

Plan Objectives - IOS LWD U-PLAN MET Series

IOS LWD U-PLAN (Plan Achromatic) MET objectives represent the stateof-the-art lenses for both upright and inverted microscopes, specical-

LWD

U-PLAN

MET

∞

ly delivering excellent performance in the metallurgical eld without the need of the cover slide, being NCG (no cover glass). Long Working Distance provides a wider working space between the objective front lens and the specimen, a benet for a variety of samples.

They are designed to ensure eld atness up to F.N. 25.

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

IOS LWD U-PLAN MET objectives are designed for IM-3, inverted and

upright modular systems used in metallurgical applications.

IOS

14

BF

PO

25

DIC

CODE MAG.

M-1100 5x 0.15 10.8 25 ∞ -

M-1101 10x 0.30 10 25 ∞ -

M-1102 20x 0.45 4 25 ∞ -

M-1103 50x 0.55 7.9 25 ∞ -

M-1104 100x 0.80 2.1 25 ∞ -

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

Plan Objectives - IOS LWD U-PLAN MET BD Series

IOS LWD U-PLAN (Plan Achromatic) MET BD objectives represent the state-of-the-art lenses for inverted and upright microscopes, specically

LWD U-PLAN MET BD

∞

IOS LWD U-PLAN MET BD objectives are designed for inverted and upright modular systems used in metallurgical applications with darkeld.

delivering excellent performance in the material science eld both for brighteld and darkeld techniques, without the need of cover glass, being NCG (no cover glass). Long Working Distance provides a wider working space between the objective front lens and the specimen, a benet for a variety of samples. .

They are designed to ensure eld atness up to F.N. 25, based on inni ty-corrected optical system.

IOS

25

DFBF

CODE MAG.

M-1094 5x 0.15 9 25 ∞ - M26

M-1095 10x 0.30 9 25 ∞ - M26

M-1096 20x 0.45 3.4 25 ∞ - M26

M-1097 50x 0.55 7.5 25 ∞ - M26

M-1098 100x 0.80 2 25 ∞ - M26

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

DICPO

Plan Semi-APO Objectives - IOS W-PLAN F Series

IOS W-PLAN (Plan Semi-Apochromatic) F objectives are great to detect uorescence, even in case of weak signals which will result very clear and visible. They combine superior performance especially in uorescence with

W-PLAN F

∞

enhanced contrast, matching all the requirements of labs requiring specic lenses for B, G and UV uorescence. PLAN-Fluorite (or Semi-Apochromatic) design ensures additional sphe rical aberration correction for superior resolution and greater numerical apertures.

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

IOS W-PLAN F objectives are designed for B-510 and upright modular systems.

IOS

BF

FL

22

DF

FL UV

CODE MAG.

M-1060 4x 0.13 4.7 22 ∞ 0.17

M-1061 10x 0.30 4.1 22 ∞ 0.17

M-1062 20x 0.50 1.45 22 ∞ 0.17

M-1063 40x 0.75 0.5 22 ∞ 0.17 O

M-1064 100x 1.30 0.08 22 ∞ 0.17 OIL O

NUMERICAL

APERTURE

They are designed to ensure eld atness up to F.N. 22, based on inni ty-corrected optical system.

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

15

Plan Semi-APO Objectives - IOS U-PLAN F Series

IOS U-PLAN (Plan Semi-Apochromatic) F objectives represent the state- of-the-art lenses for upright microscopes, specically developed for topclass performance and contrast required by the most demanding users. The PLAN-Fluorite (or Semi-Apochromatic) design ensures

U-PLAN F

∞

additional spherical aberration correction for superior resolution and greater numeri cal apertures. A superb resolution and contrast is granted especially for uorescence applications, being very eective with UV uorescence, but their extended versatility makes them an excellent product for other microscopic techni ques.

IOS U-PLAN F objectives are designed for upright modular systems.

They are designed to ensure eld atness up to F.N. 25.

IOS

25

COVERGLASS

THICKNESS

(mm)

BF

DIC

FL

DF

PO

FL UV

CODE MAG.

M-1075 4x 0.13 16.5 25 ∞ 0.17

M-1076 10x 0.30 8.1 25 ∞ 0.17

M-1077 20x 0.50 2.1 25 ∞ 0.17

M-1078 40x 0.75 0.7 25 ∞ 0.17 O

M-1079 100x 1.30 0.15 25 ∞ 0.17 OIL O

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

Plan Semi-APO Objectives - IOS LWD U-PLAN F Series

IOS LWD U-PLAN (Plan Semi-Apochromatic) F objectives represent the state-of-the-art

LWD

U-PLAN F

∞

IOS LWD U-PLAN F objectives are designed for IM-3 and inverted modular systems.

lenses for inverted microscopes, specically developed for top-class performance and contrast required by the most demanding users. Long Working Distance provides a wider working space between the objective front lens and the specimen, a benet for a variety of samples , whilst the PLAN-Fluorite (or Semi-Apochromatic) design ensures additional sphe rical aberration correction for superior resolution and greater numerical apertures. A superb resolution and contrast is granted especially for uorescence applications, being very eective with UV uorescence. They are designed to ensure eld atness up to F.N. 25.

IMMERSION SPRING REMARKS

IOS

BF FL

FL UV

16

25

CODE MAG.

M-800 4X 0.13 18.52 25 ∞ 1.2

M-801 10x 0.30 7.11 25 ∞ 1.2

M-802 20x 0.45 5.91 25 ∞ 1.2

M-803 40x 0.65 1.61 25 ∞ 1.2

M-804 60x 0.75 1.04 25 ∞ 1.2

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

Plan Semi-APO Objectives - IOS LWD U-PLAN F PH Series

IOS LWD U-PLAN (Plan Semi-Apochromatic) F PH objectives represent the state-of-the-art lenses for

LWD

U-PLAN F

PH

∞

IOS LWD U-PLAN F PH objectives are designed for IM-3 and inverted modular systems.

inverted microscopes, specically developed for top-class performance and contrast required by the most demanding users. Long Working Distance provides a wider working space between the objective front lens and the specimen, a benet for a variety of samples, whilst the PLAN-Fluorite (or Semi-Apochromatic) design ensures additional spherical aberration correction for superior resolution and greater numerical apertures. A superb resolution and contrast is granted especially for the observation of culture specimens in (positive) phase contrast method, but their versatility makes them an excellent product for other microscopic techniques, being very eective with UV uorescence. They are designed to ensure eld atness up to F.N. 25.

IOS

25

COVERGLASS

THICKNESS

BF

PH

DF

FL

CODE MAG.

M-1177 20x 0.45 5.91 25 ∞ 1.2

M-1178 40x 0.65 1,61 25 ∞ 1.2

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

FL UV

Plan Semi-APO Objectives - IOS U-PLAN F PH Series

IOS U-PLAN (Plan Semi-Apochromatic) F PH objectives represent the state-of-the-art lenses for upright microscopes, specically delivering excellent performance in the biological eld, when superb quality of the

U-PLAN

F PH

∞

image is needed working in Phase Contrast. The PLAN-Fluorite (or Semi-Apochromatic) design ensures additional spherical aberration correction for superior resolution and greater numerical apertures. They are designed to ensure eld atness up to F.N. 25.

(mm)

IMMERSION SPRING REMARKS

IOS U-PLAN F PH objectives are designed for inverted and upright modular systems used in biological applications.

IOS

25

NUMERICAL

APERTURE

PH

DFBF

PO

FL UV

CODE MAG.

M-1310 4x 0.13 16.6 25 ∞ 0.17 *

M-1311 10x 0.40 2.5 25 ∞ 0.17 *

M-1312 20x 0.75 0.6 25 ∞ 0.17 *

M-1313 40x 0.95 0.15 25 ∞ 0.17 O *

M-1314 60x 0.90 0.26 25 ∞ 0.17 O *

M-1315 100x 1.35 0.13 25 ∞ 0.17 OIL O *

* The use of M-1157 condenser and dedicated phase rings is required

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

17

Plan Semi-APO Objectives - IOS LWD U-PLAN F MET Series

IOS LWD U-PLAN (Plan Semi-Apochroma- tic) F MET objectives represent the state-of-the-art len-

LWD

U-PLAN F

MET

∞

IOS LWD U-PLAN F MET objectives are designed for inverted and upright modular systems used in metallurgical applications.

ses for upright and inverted microscopes, specically delivering excellent performance in the metallurgical eld without the need of the cover slide, being NCG (no cover glass). Long Working Distance provides a wider working space between the objective front lens and the specimen, a benet for a variety of samples. The PLAN-Fluorite (or Semi-Apochromatic) design ensures additional spherical aberration correction for superior resolution and greater numeri cal apertures. They are designed to ensure eld atness up to F.N. 25.

IOS

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

BF

DIC

25

PO

CODE MAG.

M-1171 5x 0.15 19.5 25 ∞ -

M-1172 10x 0.30 10.9 25 ∞ -

M-1173 20x 0.50 3.2 25 ∞ -

M-1174 50x 0.80 1.2 25 ∞ -

M-1175 100x 0.90 1 25 ∞ -

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

Plan Semi-APO Objectives - IOS LWD U-PLAN F MET BD Series

IOS LWD U-PLAN (Plan Semi-Apochromatic) F MET BD objectives represent the state-of-the-art lenses for upright and inverted microscopes, specically

LWD

U-PLAN F

MET BD

∞

delivering excellent performance in the material science eld both for brighteld and darkeld techniques, without the need of cover glass, being NCG (no cover glass). Long Working Distance provides a wider working space between the objective front lens and the specimen, a benet for a variety of samples. The PLAN-Fluorite (or Semi-Apochromatic) design ensures additional spherical aberration correction for superior resolution and greater numeri cal apertures. They are designed to ensure eld atness up to F.N. 25.

IOS LWD U-PLAN F MET BD objectives are designed for inverted and

upright modular systems used in metallurgical applications with darkeld.

IOS

NUMERICAL

APERTURE

BF DF

PO DIC

18

25

CODE MAG.

M-1180 5x 0.15 13.5 25 ∞ - M26

M-1181 10x 0.30 9 25 ∞ - M26

M-1182 20x 0.50 2.5 25 ∞ - M26

M-1183 50x 0.80 1 25 ∞ - M26

M-1184 100x 0.90 1 25 ∞ - M26

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

Plan APO Objectives - IOS U-PLAN APO Series

IOS U-PLAN APO (Plan Apochromatic) objectives represent the stateof-the-art lenses for upright microscopes, specically delivering excellent

U-PLAN

APO

∞

IOS U-PLAN APO objectives are designed for upright modular systems in biological applications.

performance in the biological research eld. The U-PLAN APO design ensures theoretical spherical aberration correction for superior resolution and greater numerical apertures. They are designed to ensure eld atness up to F.N. 25.

IOS

PO

FL

25

DFBF

DIC

FL UV

CODE MAG.

M-1301 2X 0.08 6.2 25 ∞ 0.17

M-1302 4X 0.13 16.6 25 ∞ 0.17

M-1303 10X 0.40 2.1 25 ∞ 0.17

M-1304 20X 0.75 0.6 25 ∞ 0.17

M-1305 40X 0.95 0.15 25 ∞ 0.17 O

M-1306 60X 0.90 0.26 25 ∞ 0.17 O

M-1307 100X 1.35 0.13 25 ∞ 0.17 OIL O

NUMERICAL

APERTURE

W.D.

(mm)

F.N.

OPTICAL

SYSTEM

COVERGLASS

THICKNESS

(mm)

IMMERSION SPRING REMARKS

19

Objective Benchmark Table

OPTICAL

CORRECTION

ACHROMATIC

OPTICAL

CORRECTION

PLAN

OBJECTIVE SERIES CODE MAGNIFICATION

M-131 4x 0.10 18 18 160 0.17

M-132 10x 0.25 7 18 160 0.17

ACH

HC

OBJECTIVE SERIES CODE MAGNIFICATION

N-PLAN

IOS N-PLAN

IOS N-PLAN POL

IOS W-PLAN

W-PLAN PH

IOS W-PLAN PH

IOS LWD W-PLAN M-1099 2.5x 0.08 11.3 22 ∞ -

IOS W-PLAN MET

IOS U-PLAN POL

M-133 20x 0.40 2 18 160 0.17

M-134 40x 0.65 0.53 18 160 0.17

M-135 60x 0.80 0.13 18 160 0.17

M-136 100x 1.25 0.13 18 160 0.17 OIL

M-137 4x 0.10 18 18 160 0.17

M-138 10x 0.25 7 18 160 0.17

M-139 20x 0.40 2 18 160 0.17

M-141 40x 0.65 0.53 18 160 0.17

M-142 60x 0.80 0.45 18 160 0.17

M-143 100x 1.25 0.13 18 160 0.17 OIL /WATER

M-164 4x 0.10 15.2 20 160 0.17

M-165 10x 0.25 5.5 20 160 0.17

M-166 20x 0.40 3.5 20 160 0.17

M-167 40x 0.65 0.45 20 160 0.17

M-168 60x 0.85 0.45 20 160 0.17

M-169 100x 1.25 0.13 20 160 0.17 OIL /WATER

M-144 4x 0.10 16.8 20 ∞ 0.17

M-145 10x 0.25 5.8 20 ∞ 0.17

M-146 20x 0.40 5.1 20 ∞ 0.17

M-147 40x 0.65 0.43 20 ∞ 0.17

M-149 60x 0.80 0.14 20 ∞ 0.17

M-148 100x 1.25 0.13 20 ∞ 0.17 OIL /WATER

M-144 4x 0.10 16.8 20 ∞ 0.17

M-145 10x 0.25 5.8 20 ∞ 0.17

M-146 20x 0.40 5.1 20 ∞ 0.17

M-147 40x 0.65 0.43 20 ∞ 0.17

M-149 60x 0.80 0.14 20 ∞ 0.17

M-148 100x 1.25 0.13 20 ∞ 0.17 OIL /WATER

M-1049 2x 0.08 19.4 22 ∞ 0.17

M-1125 4x 0.10 17.3 22 ∞ 0.17

M-1126 10x 0.25 10 22 ∞ 0.17

M-1127 20x 0.40 5.1 22 ∞ 0.17

M-1128 40x 0.65 0.54 22 ∞ 0.17

M-634.1 50x 0.95 0.19 22 ∞ 0.17 OIL

M-1129 60x 0.80 0.14 22 ∞ 0.17

M-1130.1 100x 0.36 - 1.25 0.18 22 ∞ 0.17 OIL

M-1130 100x 1.25 0.13 22 ∞ 0.17 OIL

M-170 10x 0.25 12.2 22 160 0.17

M-171 20x 0.40 5 22 160 0.17

M-172 40x 0.65 0.37 22 160 0.17

M-182 100x 1.25 0.13 22 160 0.17 OIL

M-1120.N 10x 0.25 10 22 ∞ 0.17

M-1121.N 20x 0.40 5.1 22 ∞ 0.17

M-1122.N 40x 0.65 0.54 22 ∞ 0.17

M-1123.N 100x 1.25 0.13 22 ∞ 0.17 OIL

M-337 4x 0.10 17.3 22 ∞ -

M-336 5x 0.12 15.5 22 ∞ -

M-338 10x 0.25 10 22 ∞ -

M-339 20x 0.40 5.8 22 ∞ -

M-335 50X 0.75 0.32 22 ∞ -

M-698.2 100x 0.80 3.2 22 ∞ -

M-1080 4x 0.10 20.8 22 ∞ 0.17

M-1081 10x 0.25 5.3 22 ∞ 0.17

M-1081.5 20x 0.45 1.56 22 ∞ 0.17

M-1082 40x 0.65 0.36 22 ∞ 0.17

M-1083 60x 0.85 0.30 22 ∞ 0.17

NUMERICAL

APERTURE

NUMERICAL

APERTURE

WORKING

DISTANCE (mm)

WORKING

DISTANCE (mm)

FIELD NUMBER

(mm)

FIELD NUMBER

(mm)

OPTICAL

SYSTEM

OPTICAL

SYSTEM

COVER GLASS

THICKNESS (mm)

COVER GLASS

THICKNESS (mm)

20

IMMERSION SPRING BF DF DIC PH RPC PO FL (B,G) FL (UV) SCREW THREAD REMARKS

««

l ««

«« «

l «« «

IMMERSION SPRING BF DF DIC PH RPC PO FL (B,G) FL (UV) SCREW THREAD REMARKS

«« ««

l «« ««

«« ««

l «« ««

«« ««

l «« ««

«« ««

l «« ««

«« ««

l «« ««

«« ««

l «« ««

««

l ««

««

«« ««

l «« ««

- - - - - - - RMS

- - -

- - - - - - RMS

- - - -

- - -

- - - -

-

«««

-

«««

-

«««

-

«««

-

«««

-

«««

-

«««

-

«««

- - - -

- - -

-

«

«« ««

«

«««

««

««« ««

- - RMS

««

- - RMS

- - RMS DEPOLARIZED

- - RMS M-860.1 is required

- - RMS M-860 is required

- - RMS

- RMS

- RMS IRIS

- RMS

«««: Very Good ««: Good «: Usable - : Not Good ¶: Some Limitations

21

Objective Benchmark Table

OPTICAL

CORRECTION

PLAN

OBJECTIVE SERIES CODE MAGNIFICATION

M-1131 4x 0.10 17.3 22 ∞ 0.17

M-1132 10x 0.25 10.0 22 ∞ 0.17

IOS W-PLAN POL

IOS LWD W-PLAN POL

IOS LWD W-PLAN

IOS LWD W-PLAN PH

IOS LWD U-PLAN RPC

IOS LWD U-PLAN POL

IOS LWD U-PLAN MET

IOS LWD U-PLAN MET BD

M-1133 20x 0.45 0.40 22 ∞ 0.17

M-1134 40x 0.65 0.54 22 ∞ 0.17

M-1135 60x 0.80 0.14 22 ∞ 0.17

M-1136 5x 0.12 15.5 22 ∞ -

M-1137 10x 0.25 10.0 22 ∞ -

M-1138 20x 0.40 5.8 22 ∞ -

M-1139 50x 0.75 0.32 22 ∞ -

M-782 10x 0.13 10.4 22 ∞ 1.2

M-773 40x 0.60 3.10 22 ∞ 1.2

M-786 60x 0.70 1.70 22 ∞ 1.2

M-782.1 4x 0.13 10.4 22 ∞ 1.2

M-783N 10x 0.25 7.3 22 ∞ 1.2

M-784N 20x 0.40 6.8 22 ∞ 1.2

M-785 40x 0.65 3.00 22 ∞ 1.2

M-861 4X 0.13 10.75 22 ∞ 1.2

M-862 10x 0.25 7.45 22 ∞ 1.2

M-863 20x 0.40 6.92 22 ∞ 1.2

M-864 40x 0.65 2.74 22 ∞ 1.2

M-1090 5x 0.15 10.8 25 ∞ -

M-1091 10x 0.30 10 25 ∞ -

M-1092 20x 0.45 4 25 ∞ -

M-1093 50x 0.55 7.9 25 ∞ -

M-1100 5x 0.15 10.8 25 ∞ -

M-1101 10x 0.30 10 25 ∞ -

M-1102 20x 0.45 4 25 ∞ -

M-1103 50x 0.55 7.9 25 ∞ -

M-1104 100x 0.80 2.1 25 ∞ -

M-1094 5x 0.15 9 25 ∞ -

M-1095 10x 0.30 9 25 ∞ -

M-1096 20x 0.45 3.4 25 ∞ -

M-1097 50x 0.55 7.5 25 ∞ -

M-1098 100x 0.80 2 25 ∞ -

NUMERICAL

APERTURE

WORKING

DISTANCE (mm)

FIELD NUMBER

(mm)

OPTICAL

SYSTEM

COVER GLASS

THICKNESS (mm)

22

IMMERSION SPRING BF DF DIC PH RPC PO FL (B,G) FL (UV) SCREW THREAD REMARKS

«« ««

l «« ««

««

«««

««« ««« ««¶

««« ««« «««

- - -

- - - -

- - - - -

- -

- - -

- - - -

-

««¶

-

«««

-

«««

-

«««

-

«««

- -

««« ««

- -

«««

«« ««

««

«««

- - - RMS

- - RMS

««

- - M26

- RMS

«««: Very Good ««: Good «: Usable - : Not Good ¶: Some Limitations

23

Objective Benchmark Table

OPTICAL

CORRECTION

PLAN

SEMI APO

OBJECTIVE SERIES CODE MAGNIFICATION

M-1060 4x 0.13 4.7 22 ∞ 0.17

M-1061 10x 0.30 4.1 22 ∞ 0.17

IOS W-PLAN F

IOS U-PLAN F

IOS LWD U-PLAN F

IOS LWD U-PLAN F PH

IOS U-PLAN F PH

IOS LWD U-PLAN F MET

IOS LWD U-PLAN F MET BD

M-1062 20x 0.50 1.45 22 ∞ 0.17

M-1063 40x 0.75 0.5 22 ∞ 0.17

M-1064 100x 1.30 0.08 22 ∞ 0.17 OIL

M-1070 4x 0.13 16.43 25 ∞ 0.17

M-1071 10x 0.30 8.13 25 ∞ 0.17

M-1072 20x 0.50 2.03 25 ∞ 0.17

M-1073 40x 0.75 0.74 25 ∞ 0.17

M-1073.1 60x 0.90 0.26 25 ∞ 0.17

M-1074 100x 1.28 0.18 25 ∞ 0.17 OIL

M-1075 4x 0.13 16.5 25 ∞ 0.17

M-1076 10x 0.30 8.1 25 ∞ 0.17

M-1077 20x 0.50 2.1 25 ∞ 0.17

M-1078 40x 0.75 0.7 25 ∞ 0.17

M-1079 100x 1.30 0.15 25 ∞ 0.17 OIL

M-800 4X 0.13 18.52 25 ∞ 1.2

M-801 10x 0.30 7.11 25 ∞ 1.2

M-802 20x 0.45 5.91 25 ∞ 1.2

M-803 40x 0.65 1.61 25 ∞ 1.2

M-804 60x 0.75 1.04 25 ∞ 1.2

M-1177 20x 0.45 5.91 25 ∞ 1.2

M-1178 40x 0.65 1,61 25 ∞ 1.2

M-1310 4x 0.13 16.6 25 ∞ 0.17

M-1311 10x 0.40 2.5 25 ∞ 0.17

M-1312 20x 0.75 0.6 25 ∞ 0.17

M-1313 40x 0.95 0.15 25 ∞ 0.17

M-1314 60x 0.90 0.26 25 ∞ 0.17

M-1315 100x 1.35 0.13 25 ∞ 0.17 OIL

M-1171 5x 0.15 19.5 25 ∞ -

M-1172 10x 0.30 10.9 25 ∞ -

M-1173 20x 0.50 3.2 25 ∞ -

M-1174 50x 0.80 1.2 25 ∞ -

M-1175 100x 0.90 1 25 ∞ -

M-1180 5x 0.15 13.5 25 ∞ -

M-1181 10x 0.30 9 25 ∞ -

M-1182 20x 0.50 2.5 25 ∞ -

M-1183 50x 0.80 1 25 ∞ -

M-1184 100x 0.90 1 25 ∞ -

NUMERICAL

APERTURE

WORKING

DISTANCE (mm)

FIELD NUMBER

(mm)

OPTICAL

SYSTEM

COVER GLASS

THICKNESS (mm)

OPTICAL

CORRECTION

PLAN APO

24

OBJECTIVE SERIES CODE MAGNIFICATION

M-1301 2X 0.08 6.2 25 ∞ 0.17

M-1302 4X 0.13 16.6 25 ∞ 0.17

M-1303 10X 0.40 2.1 25 ∞ 0.17

IOS U-PLAN APO

M-1304 20X 0.75 0.6 25 ∞ 0.17

M-1305 40X 0.95 0.15 25 ∞ 0.17

M-1306 60X 0.90 0.26 25 ∞ 0.17

M-1307 100X 1.35 0.13 25 ∞ 0.17 OIL

NUMERICAL

APERTURE

WORKING

DISTANCE (mm)

FIELD NUMBER

(mm)

OPTICAL

SYSTEM

COVER GLASS

THICKNESS (mm)

IMMERSION SPRING BF DF DIC PH RPC PO FL (B,G) FL (UV) SCREW THREAD REMARKS

««« ««

««« «« «

l ««« «« «

««« «««

l ««« «««

««« «««

««« ««« ««¶

««« ««« «««

l ««« ««« «««

«««

««« ««

««« ««« « «««

««« ««« ««« «««

l ««« ««« ««« «««

«««

««« ««« ««¶

««« ««« «««

- - - - -

-

- - - -

- - -

- -

-

«««

-

«««

««¶

«««

- -

-

««« «««

«« ««« «««

««« «««

«« ««« «««

««

- - RMS

- - M26

RMS

IMMERSION SPRING BF DF DIC PH PO FL (B,G) FL (UV) SCREW THREAD REMARKS

«««

««« ««

««« «« «««

l ««« «« «««

l «««

«««: Very Good ««: Good «: Usable - : Not Good ¶: Some Limitations

- - - -

- - -

- -

- - - -

«« ««« «««

RMS

25

Note

26

27

Rev 30.1.2022 - OPTIKA reserves the right to make corrections, modications, enhancements, improvements and other changes to its products at any time without notice.

Headquarters and Manufacturing Facilities

OPTIKA S.r.l.

®

Via Rigla, 30 - 24010 Ponteranica (BG) - ITALIA - Tel.: +39 035.571.392

Optika Sales branches

OPTIKA Spain OPTIKA China

® ®

spain@optikamicroscopes.com

china@optikamicroscopes.com

- info@optikamicroscopes.com

OPTIKA USA OPTIKA India

usa@optikamicroscopes.com

india@optikamicroscopes.com

Optika M-1120.N Booklet [EN]

Specifications and Main Features

Frequently Asked Questions

User Manual