Optika IM, IM-3FL4, IM-3F Instruction Manual
v 1.0 2017
Model
IM-3F
IM-3FL4
IM Series
INSTRUCTION MANUAL
Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
Overview
Unpacking
Assembling
Using the microscope
Maintenance
Troubleshooting
Equipment disposal
Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
Page 4
Overview
TRINOCULAR
VIEWING
TUBE
(FIXED)
DIOPTRIC
ADJUSTMENT
RING
EYEPIECE
PHASE CONTRAST SLIDER
(OPTIONAL FOR IM-3FL4)
LED
HOUSING
CONDENSER
FILTER HOLDER
COARSE
FOCUS KNOB
FINE FOCUS KNOB
FLUORESCENCE
POWER SUPPLY
TENSION
ADJUSTMENT
COLLAR
OBJECTIVE
STAGE
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
GLASS STAGE INSERT
Page 5
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Assembling
Once you open the box, these are the microscope’s components:
① Microscope body
② Condenser
③ LED illuminator
④ Fluorescence power supply
⑤ Power cables
⑥ Filter holder
⑦ Metal insert for stage
⑧ Glass insert for stage
⑨ Objectives
⑩ Eyepieces
⑪ Fluorescence lters
⑫ Brighteld lters (IF550)
⑬ Orange screen
⑭ Diaphragm assembly
⑮ Mercury lamp house
①
②
⑪
⑫
⑬ ⑭
⑮
⑩
③
④
⑤
⑥
⑦ ⑧
⑨
Page 6
③
Fig.2
Fig.3
Fig.4
Fig.5
Fig.1
1. Turning the coarse focusing knob ① till the
nosepiece reaches its lowest position.
► For a safe transport, the nosepiece is
placed in the lowest position and the
tension adjustment collar ② is adjust-
ed to the appropriate tension when the
microscope leaves the factory. (Fig.1)
2. Screw the lowest magnication objective
on to the turret from the right side, then
turn the turret clockwise. Mount the other
objectives in the same way, following the
sequence from low to high.
► Note: the objectives can also be in-
stalled through the stage opening.
(Fig.2)
► Clean the objectives regularly. In inverted
microscopes, the objectives are very sensitive to dust.
► To prevent dust and contamination from
entering the microscope, cover all the unused holes with dust caps ③. (Fig.3)
► When operating, use the low magnication
objective (10X) to search and focus the
specimen, then switch to higher magnica-
tions.
► When switching between objectives, slowly
turn the nosepiece until it clicks. The click
means that the objective is in the right position, in the center of the light path.
①
②
Installing the objectives
The stage extension can be installed on either
side of the stage to enlarge the working surface. The mechanical stage must be installed
on the side opposite the extension.
For right-handed operators, the mechanical
stage is normally installed on the right side.
1. Installing the stage extension: Screw the
bolts on to the extension, then mount the
extension from below the stage. (Fig.4)
2. Installing the mechanical stage: As for the
extension, the mechanical stage is xed
with two bolts under the stage. (Fig.5)
Installing the stage extension and
the mechanical stage (OPTIONAL)
Page 7
Page 7
1. When using the glass stage, make sure
that the insert is horizontal.
2. Install the stage insert in the stage open-
ing. (Fig.6)
Installing the stage insert
Installing the eyepieces
Insert both eyepieces into the tubes of the
optical head. (Fig.7)
Fig.6
Fig.7
Fig.8
Fig.9
Fig.10
1. Insert the condenser illumination unit into
the bracket. (Fig.8)
2. Turn the condenser illumination unit clockwise about 90°, with the “AS” mark of lter
holder facing forwards. Align the screw of
the condenser illumination unit and the
hole of the holder, then screw the bolt in
the hole with the supplied allen wrench.
(Fig.9)
3. Insert the connector plug into the connec-
tor jack.
4. Push the LED housing gently into the
holes of the illumination unit. (Fig.10)
Installing the condenser
illumination unit and the LED
housing
Page 8
► Remove the lter holder, then install the
color lters you need. (Fig.11, Fig.12)
Mount the color lter at as shown in ①,
verifying that they are not tilted.
► If the color lter is tilted or otherwise
out of place ②, it may fall.
The color lters can be stacked in the
holder. This allows to install as many lters
as needed, as long as the whole thickness
is less than 11 mm.
• Pull the black plastic cover out, from the
microscope rear. (Fig.13)
• Insert the lens/diaphragm assembly from
the back. In order to ease the insertion,
just tilt the assembly at about 45° and
move it forward. Fix it using the 3 provided
allen screws. (Fig.14)
• Insert the lamp house and x it with the allen screw (already inside the support tube
(1) ). (Fig.15)
Installing the color lters
Installing the uorescence
Fig.11
Fig.12
Fig.13
Fig.14
Fig.15
①
②
Page 9
• Insert the lter holder into its slot near the
lamp house. (Fig.16)
• Unscrew the protection cover on the left
side of the microscope, with the provided
allen wrench. (Fig.17)
• Slide the green lter inside the dovetail
guide, after screwing the lter lever into the
threaded hole of the lter. Mount the protection cover back in its position. (Fig.18)
• On top of the lter lever, screw the terminal
with the etched G. Repeat the same steps
for the right side, mounting the Blue lterset. (Fig.19)
• For preventing possible damages from
UV radiation, mount the orange protection
screen as shown. (Fig.20)
Fig.16
Fig.17
Fig.18
Fig.19
Fig.20
Page 10
• Connect the cable from the external power
supply to the HBO lamp house. (Fig.21;
Fig.22)
• Connect the power cable to the external
power supply. (Fig.23)
Fig.21
Fig.22
Fig.23
• The input voltage for uorescence power supply is 110-240Vac.
• Please use the standard power cable provided by our company. Select suitable one when mis-
sing or damaged.
• Connect the power supply correctly, be sure to have a good earth connection.
Page 11
Fig.24
Fig.25
Fig.26
Mount or replace the mercury lamp
Before replacing the bulb, please set the uorescence power supply to (OFF) position and
unplug the power cable. Be sure that the lamp
has completely cooled down.
Loosen the lock-screw ① (Fig.24) completely
and take o the bulb holder ②. Loosen the
lock screw ③ and take o the plastic pole ④.
(Fig.25)
The HBO lamp has 2 poles of dierent dimensions, so that it ts in only one direction: insert
the anode pole (bigger one) into the xed holder ① and the other pole in the exible holder.
Then tighten the screw. (Fig.26)
Mount the lamp door back in place and tighten
the door locking screw.
②
③
④
①
①
Page 12
1. Turn the main switch ① to “O”(o) before
connecting the power cord. (Fig.27)
2. Insert the cable into the power socket of
the microscope. (Fig.28)
3. Plug the power cord into the mains sock-
et. Check for a safe connection.
► Please use the supplied power cord.
If lost or damaged, please refer to
qualied service.
► Connect the power cord to a grounded
(earthed) power supply only.
Before replacing the fuse, turn the main switch
to “O” (o) and unplug the power cord.
Rotate the fuse support out of the holder using
a straight screwdriver. Insert a new fuse in the
support, then rotate the support back into the
holder. (Fig.29)
► Fuse rating:
see back of the microscope.
Connecting the power cord
Replacing the fuse
Fig.27
Fig.28
Fig.29
①
Page 13
Turning on the LED
Connect the power, turn on the main switch ①.
(Fig.30)
Adjusting the brightness
Turn the brightness adjustment knob ② to in-
crease and decrease the brightness. (Fig.31)
Adjusting the tension
► The coarse focusing knob ① is pre
adjusted to a tight tension upon leaving the factory.
If the nosepiece drops down by itself, or the
specimen defocuses while adjusting the ne
focus knob ③, the coarse focus knob is too
loose. Turning the tension adjustment collar ②
in clockwise direction tightens the coarse focus tension ①. Rotate in the opposite direction
to decrease the tension. (Fig.32)
Setting the specimen
► For the best image quality, use asks,
Petri dishes and slides with a 1.2 mm
thickness.
1. Place the proper insert for your specimen
(according to the table on the right) on the
stage, and x it with the stage clip.
2. Turning the X and Y knobs, move the
specimen to the required position. (Movement Range: 120 (width) × 78 (length)
mm).
Moving the specimen
Move the specimen to the desired position by
freehand or by turning the knobs of the mechanical stage.
► When switching objectives, take care
not to touch the adaptor plates with
the objectives, as their weight may
damage the front lens.
M-793.1
Holder for Petri diameter 38mm (M-793.2 needed)
M-793.2
Holder for Terasaki and Petri diameter 65mm.
M-793.3
Holder for slide and Petri diameter 54mm.
M-793.4
Holder for 2+2 slides.
M-793.6
Holder for Utermöhl-Chamber (M-793.3 needed).
M-792
Mechanical stage for IM-3 series.
INITIAL SETUP
STAGE (OPTIONAL)
Using the microscope
Fig.30
Fig.31
①
②
①
②
③
Fig.32
M-793.7
Load-bearing side extension for IM-3 series.
Page 14
Dioptric adjustment
1. Look into the right eyepiece with your right
eye only, and focus on the specimen.
2. Look into the left eyepiece with your left
eye only. If the image is not sharp, use
the dioptric adjustment ring ① to compen-
sate. (Fig.33)
► The adjustment range is ±5 diopter.
The number indicated on the adjustment ring graduation should correspond to the operator’s dioptric correction.
Adjusting the interpupillary distance
Observing with both eyes, hold the two eyepiece prism assemblies. Rotate them around
their common axis until the elds of view coincide.
► The graduation on the interpupillary
distance indicator ②, pointed by the
spot “.” on the eyepiece holder, shows
the distance between the operator’s
eyes. (Fig.34)
The range of the interpupillary distance is 48-
75mm.
Selecting the light path
Pull the light path selector lever ③ sideways
using your thumb, selecting the light path you
need. (Fig.35)
VIEWING TUBE
②
Fig.33
Fig.34
Fig.35
LIGHT PATH SELECTOR
LEVER
BRIGHTNESS APPLICATION
In 20% used for binocular observation, and
80% used for video or photography
Binocular observation, television, and
micrography or video can be operated
simultaneously
Out 100% used for binocular
observation
Binocular
observation
③
①
Page 15
Using color lters
Selecting the appropriate color lters according your need. (Fig.36)
You can stack a group of color lters in the lter holder, if you ensure that they are level and
that the whole thickness is less than 11mm.
Using the aperture diaphragm
When in brighteld observation, the aperture
diaphragm controls the numerical aperture of
the illumination system. When the numerical
aperture of the objective and the aperture of
the illumination system match, the highest resolution is achieved.
The aperture can be changed by moving the
aperture adjustment lever. ① is the image of
the aperture diaphragm, ② is the edge of the
objective).
Generally, when observing a fully chromatic
specimen, you need to set the size of the condenser to 70-80% of the aperture of the objective. When observing unstained samples (e.g.
bacteria), start from 70% and slowly turn the
aperture diaphragm lever clockwise. (Fig.37)
ILLUMINATION UNIT
Phase contrast slider
Adjustable phase slider.
● The light ring is pre-centered when the
microscope leaves the factory. It should
therefore need no further adjustment. If a
recentering is needed, it can be performed
via the two side bolts.
● The 4x/10x light ring ① must be used with
4x and 10x phase contrast objectives, the
20x/40x light ring ② with the 20x and 40x
and the opening ③ is used for bright eld.
(Fig.38)
Installing the phase contrast slider
1. Insert the slider into the illumination
system, printed face up.
2. Pull the slider into the desired position, to
the click stop.
3. When in phase contrast observation, keep
the aperture diaphragm adjustment lever
on the “O” (open) position. (Fig.39)
PHASE CONTRAST
(
OPTIONAL FOR IM-3FL4
)
Fig.36
Fig.38
Fig.39
Fig.37
70-
30-
70-80%
30-20%
COLOR
FILTER
USE
Green Single contrast color lter used for phase
contrast microscopy
①
①
②
②
③
Page 16
Fig.40
Fig.41
Fig.42
Centering the ring
► Usually this operation is not needed. If
necessary, please proceed with the following steps:
1. Place a specimen on the stage and focus
it.
2. Take out the eyepiece from the tube without the dioptric adjustment, and replace it
with the centering telescope (CT). (Fig.40)
3. Check that the phase ring and the objective
correspond, and that both are steadily set
on a click stop.
4. Use the CT to focus on the light ring’s im-
age ① and the phase contrast ring’s image
②. If the light ring’s image is not sharp, ad-
just the CT’s eyepiece until you can see a
clear image of the light ring.
5. Adjust the bolts of the two centering holes
in the phase contrast slider using a screwdriver until the light ring center and the
phase contrast ring center coincide.
6. The 10x and the 20x phase contrast objectives use the same ring on the phase contrast slider. The coincidence of the light ring
center and the phase contrast center must
be veried with both objectives.
(Fig.41; Fig.42)
► If the light ring is centered incorrectly,
the contrast will be severely impaired.
► The phase ring may need recentering
during and after observation of very
thick specimens.
► The phase ring may show an apparent
misalignment if the cover glass is not
at.
①
②
Page 17
Fig.45
Fig.43
Fig.44
Centering mercury bulb
After turning on the uorescence power
supply, let the HBO lamp reach the thermal
stability (at least 5 minutes) before pro-
ceeding to the alignment.
Turn the nosepiece to an empty position without objective, and place a piece of white paper
directly on the hole. (Fig.43)
Pull the lter selection lever until the blue lterset is inserted into the light path.
Open the eld diaphragm completely.
(Fig.44) Adjust the lamp focusing knob ①, ver-
tical adjusting screw ②, horizontal adjusting
screw ③ in order to get an image of the bulb
on the white paper, similar to Fig.45 Ⓐ.
(Fig.44) Adjust the focusing screw ④ for the
back reecting mirror, horizontal centering
screw ⑤, vertical centering screw ⑥, in order
to get an image of the bulb’s reection on the
white paper, similar to Fig.45 Ⓑ.
(Fig.44) Continue to adjust the screws of the
back reecting mirror until you obtain a symmetrical image of the bulb and its reection,
both very near the center of the light path Fig.
45 Ⓒ.
①
Ⓐ Ⓑ Ⓒ
②
③
④
⑤
⑥
Page 18
Fig.46
Fig.47
View eld diaphragm
Field diaphragm limits the light beam diameter
on the specimen plane, therefore eliminates
the stray light in order to enhance image contrast. When the eld diaphragm image is just
at the edge of the eld of view, the system can
provide the best performance.
Turn the adjusting lever ① of eld diaphragm
clock-wise to open the diaphragm, otherwise
to close it. (Fig. 46)
Adjust the screws ② at both sides of the eld
diaphragm to center the image of the diaphragm itself. (Fig. 46)
Open the eld diaphragm gradually, if the image of eld diaphragm is just inscribed to the
eld of view, this means that the eld diaphragm has been centered. (Fig.47)
In order to prevent the specimen from uorescence quenching, don’t expose the same
portion of the specimen for too long.
①
②
Installing the photography adapter
1. To activate the video port, pull the light
path selector lever to “In” position. (Fig.48)
2. Loosen the locking bolt ① on the trinocular viewing tube, and take out the dust cap
②.
3. Install the photography adapter into the
trinocular port according to its instructions,
and screw down the locking bolt ①.
4. Attach the camera ring (if any) to the
adapter.
5. Attach the camera to the ring.
● Warning: for some cameras (mainly reex) the ring is not included with the microscope, and it should be supplied by the
user.
● For the photography of dark specimens,
obscure the eyepieces and the viewnder
with a dark cloth in order to reduce stray
light.
● The camera magnication can be calculated as objective magnication × camera
+ lens magnication.
► When shooting with a SLR, the mirror
movement may cause camera movement. Please lift the mirror, use long
exposure times and use an extension
cord. (Fig.49)
MICROPHOTOGRAPHY
Fig.48
Fig.49
①
Camera
Adapter
②
Page 19
Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of
5°-40°C and a maximum relative humidity of 75 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no
moving parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o the light immediately, cover the microscope with the included
dust-cover, and keep it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region
matches with the operation voltage of the equipment and that the lamp switch is in o-
position.
•
Users should observe all safety regulations of the region. The equipment has acquired
the CE safety label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not sucient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or
near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.
Page 20
Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
The illumination is open, but the eld
of view is dark.
The plug of the LED holder is
not connected to the illumina-
tion set
Connect them
The brightness is too low Adjust to a proper setting
Too many colour lters have
been stacked
Minimize the number of the lters
The edge of the eld of view is
vignetted or the brightness is asym-
metric.
The nosepiece is not in the correct position
Turn the nosepiece to a click stop
The color lter is partially
inserted
Insert the lter to full depth
The phase contrast slider is not
in the proper position
Move the slider to a click stop
Dust and stains can be seen in the
eld of view.
There are stains and dust on
the specimen
Clean the specimen
There are stains and dust on
the eyepiece
Clean the eyepiece
There is an apparent double image. The size of the aperture dia-
phragm is too small
Open the aperture diaphragm
Poor image quality:
The image is not sharp
The contrast is not high
The details are not clear
The phase contrast is low.
The nosepiece is not in the
center of the light path
Turn the nosepiece to a click stop
The aperture diaphragm in
the view of eld is opened too
much or too little
Adjust the aperture diaphragm
The lenses (condenser, objective, eyepieces are culture
dish) is dirty
Thoroughly clean all the optical system
In phase contrast observation,
the bottom thickness of the
sample is more than 1.2mm
Use a sample holder whose bottom thickness is less than 1.2mm
A bright eld objective is used
for phase contrast observation
Switch to a phase contrast objective
The condenser ring is not
aligned with the objective
phase ring
Adjust the condenser ring to match the
objective phase ring
The light ring and/or the phase
contrast ring is not centered
Adjust the bolts to center them
The objective used is not compatible with the phase ring
Please use a compatible objective
The phase contrast depends on
the sample position
The sample holder is not at. Move the
sample around until a compatible area is
found.
Page 21
One side of the image is out of focus. The nosepiece is not in the
center of the light path
Turn the nosepiece to a click stop
The specimen is out of place
(tilted)
Place the specimen at on the stage.
The optical performance of the
sample cover glass is poor
Use a cover glass of better quality
II. Mechanical Section:
The coarse focus knob is hard to
turn.
The tension adjustment collar is
too tight
Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is
too loose
Tighten the tension adjustment collar
III. Electric section
The LED doesn’t turn on. No power supply Check the power cord connection
The brightness is not enough The brightness adjustment is
low
Adjust the brightness
The light blinks The power cord is poorly con-
nected
Check the power cord
IV. Viewing tube assembly
The eld of view of the two eyes is
dierent
The interpupillar distance is not
correct
Adjust the interpupillar distance
The dioptric correction is not
right
Adjust the dioptric correction
The viewing technique is not
correct, and the operator is
straining the eyesight
When look into the objective, do not stare
at the specimen but look at the whole eld
of view. Periodically, move the eyes away
to look at a distant object, then back into
the objective
V. Microphotography and video
The image is unfocused Incorrect focussing Adjusting the focus system as in the pres-
ent manual
The edge of the image is unfocussed To some degree, it is inherent
to the nature of achromatic
objectives
The problem can be minimized by a correct
setting of the aperture diaphragm
Bright patches appear on the image Stray light is entering the
microscope through the eyepieces and through the camera
viewnder
Cover the eyepieces and the viewnder
with a dark cloth
Page 22
Equipment disposal
Art.13 Dlsg 25 july 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating
to the reduction in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be
collected separately from other waste.
The separate collection of this equipment at the end of its lifetime is organized and managed by the producer.
The user will have to contact the manufacturer and follow the rules that he adopted for end-of-life equipment
collection.
The collection of the equipment for recycling, treatment and environmentally compatible disposal, helps to prevent
possible adverse effects on the environment and health and promotes reuse and/or recycling of materials of the
equipment.
Improper disposal of the product involves the application of administrative penalties as provided by the laws in force.
v 1.0 2017
Modello
IM-3F
IM-3FL4
Serie IM
MANUALE D’ISTRUZIONI
Pagina 24
Indice Contenuti
Avvertenza
Simboli
Informazioni sulla sicurezza
Utilizzo previsto
Descrizione dello strumento
Disimballagio
Assemblaggio
Istruzioni per l’uso
Manutenzione
Risoluzione problemi
Smaltimento
Pagina 25
Avvertenza
Questo microscopio è uno strumento scientico di alta precisione, progettato per durare a lungo con una minima
manutenzione; la realizzazione è secondo i migliori standard ottici e meccanici, per poter essere utilizzato
quotidianamente. Vi ricordiamo che questo manuale contiene informazioni importanti per la sicurezza e per la
manutenzione dello strumento, e deve quindi essere messo a disposizione di coloro che lo utilizzeranno.
Decliniamo ogni responsabilità derivante da un utilizzo dello strumento non indicato nel presente manuale.
Simboli
La seguente tabella riporta i simboli utilizzati in questo manuale.
PERICOLO
Questo simbolo indica un rischio potenziale ed avverte di procedere con cautela.
SHOCK ELETTRICO
Questo simbolo indica un rischio di shock elettrico.
Informazioni sulla sicurezza
Per evitare shock elettrici
Prima di collegare il cavo di alimentazione alla presa elettrica, assicurarsi che il voltaggio della rete locale
coincida con il voltaggio dello strumento e che l’interruttore dell’illuminazione sia nella posizione “O”.
Gli utenti dovranno seguire tutte le norme di sicurezza locali. Lo strumento è certicato CE. In ogni caso, gli
utilizzatori sono gli unici responsabili per un utilizzo sicuro dello strumento. Per l’utilizzo in sicurezza dello
strumento è importante attenersi alle seguenti istruzioni e leggere il manuale in tutte le sue parti.
Utilizzo previsto
Solo per ricerca. Non è previsto alcun utilizzo di questo strumento per uso diagnostico.
Pagina 26
Descrizione dello strumento
TUBO DI USCITA
TRINOCULARE
ANELLO DI
REGOLAZIONE
DIOTTRICA
OCULARI
SLIDER PER
CONTRASTO DI FASE
(OPZIONALE PER IM-3FL4)
ALLOGGIAMENTO
LED
CONDENSATORE
PORTA-FILTRI
MANOPOLA DI
REGOLAZIONE DELLA
MESSA A FUOCO
MACROMETRICA
MANOPOLA DI
REGOLAZIONE
DELLA MESSA A
FUOCO
MICROMETRICA
ALIMENTATORE
FLUORESCENZA
ANELLO DI
REGOLAZIONE
DELLA
TENSIONE
OBIETTIVI
TAVOLINO
BASE DEL
MICROSCOPIO
SELEZIONATORE
PERCORSO
LUMINOSO
INSERTO PORTAPREPARATI
IN VETRO
Pagina 27
Disimballagio
Il microscopio è riposto in un imballo di polistirolo espanso. Rimuovere il nastro adesivo dal collo ed aprire la
parte superiore dell’imballo. Fare attenzione a non far cadere le parti ottiche (obiettivi e oculari) nell’estrarre il
microscopio dalla scatola per evitare che vengano danneggiati. Utilizzare entrambe le mani (una intorno allo
stativo e una alla base), slare il microscopio dal contenitore e appoggiarlo su un piano stabile.
Assemblaggio
All’apertura della scatola, i componenti del microscopio sono i seguenti:
① Stativo microscopio
② Condensatore
③ Illuminatore LED
④ Alimentatore uorescenza
⑤ Cavo di alimentazione
⑥ Porta-ltri
⑦ Portapreparati in metallo
⑧ Portapreparati in vetro
⑨ Obiettivi
⑩ Oculari
⑪ Filtri uorescenza
⑫ Filtro campo chiaro (IF550)
⑬ Schermo protettivo arancio
⑭ Blocco con diaframma
⑮ Alloggiamento lampada HBO
①
②
⑪
⑫
⑬ ⑭
⑮
⑩
③
④
⑤
⑥
⑦ ⑧
⑨
Pagina 28
③
Fig.2
Fig.3
Fig.4
Fig.5
Fig.1
①
②
1. Ruotare la manopola di regolazione macro-
metrica ① nché la torretta portaobiettivi si
trova nella posizione più bassa.
► Per garantire la sicurezza durante il tra-
sporto, prima della spedizione la torretta viene messa nella posizione più bassa e si sistema l’anello di regolazione
della tensione ② nella tensione appro-
priata. (Fig.1)
2. Avvitare l’obiettivo con minore potere di ingrandimento sulla torretta dal lato destro,
quindi ruotare la torretta in senso orario.
Montare gli altri obiettivi nello stesso modo,
dall’obiettivo con potere di ingrandimento
minore a quello maggiore.
► Nota: è possibile installare gli obiettivi
anche attraverso l’apertura del piano
portapreparati. (Fig.2)
► Tenere gli obiettivi puliti. Nei microscopi ro-
vesciati gli obiettivi sono molto sensibili alla
polvere.
► Per evitare polvere e contaminazioni, co-
prire tutti i fori non utilizzati con gli appositi
tappi antipolvere ③. (Fig.3)
► Durante l’uso, servirsi degli obiettivi con
minor potere di ingrandimento (10X) per
guardare e mettere a fuoco i preparati,
quindi aumentare il potere di ingrandimen-
to.
► Per passare da un obiettivo a un altro,
ruotare lentamente il revolver nché non
scatta. Lo scatto avverte che l’obiettivo è in
posizione corretta, al centro del percorso
luminoso.
Installazione degli obiettivi
Il prolungamento può essere montato su
entrambi i lati del piano portapreparati per
aumentare la supercie
di lavoro. Il tavolo traslatore va installato sul
lato opposto a quello del prolungamento.
Per operatori destrimani, solitamente il tavolo
traslatore si installa sul lato destro.
1. Installazione del prolungamento del piano:
Anzitutto avvitare i bulloni al prolungamento,
quindi montare il prolungamento da sotto il
piano portapreparati. (Fig.4)
2. Installazione del tavolo traslatore: Come
per il prolungamento, anche il tavolo
traslatore è ssato da due bulloni sotto il
piano. (Fig.5)
Installazione del prolungamento del
piano e del tavolo traslatore (OPZIONALE)
Pagina 29
Page 29
Fig.6
Fig.7
Fig.8
Fig.9
Fig.10
1. Assicurarsi che il piano portapreparati sia
perfettamente orizzontale quando si usa il
piano in vetro.
2. Inserire l’inserto in vetro nell’apertura del
piano. (Fig.6)
Installazione dell’inserto in vetro
Installazione degli oculari
Togliere il tappo ai tubi portaoculari, inserire gli
oculari nei tubi. (Fig.7)
1. Inserire il gruppo condensatoreilluminatore nell’apposito braccio. (Fig.8)
2. Ruotare il gruppo in senso orario di circa
90°: il marchio “AS” del portaltri deve
essere rivolto in avanti. Allineare la vite del
gruppo condensatore-illuminatore con il
foro nel portaltri, quindi avvitare il bullone
nel foro servendosi dell’apposita chiave
esagonale inclusa nel kit.. (Fig.9)
3. Inserire il cavo di alimentazione nel
connettore jack.
4. Inserire il portalampada spingendolo
con molta cura nei fori del gruppo di
illuminazione. (Fig.10)
Installazione del gruppo
condensatore-illuminatore e
dell’alloggiamento LED
Pagina 30
• Estrarre dal retro del microscopio il
coperchio plastico nero. (Fig.13)
• Inserire il blocco diaframma dal retro. Per
facilitarne l’inserimento, orientarlo a 45° e
quindi inserirlo. Fissare il blocco tramite le
3 viti a brugola fornite. (Fig.14)
• Inserire l’alloggiamento della lampada
HBO e ssarla tramite vite a brugola (che
si trova già avvitata nel tubo di supporto).
(Fig.15)
Installazione uorescenza
Fig.11
Fig.12
Fig.13
Fig.14
Fig.15
► Togliere il portaltro, quindi inserire
il ltro cromatico desiderato. (Fig.11,
Fig.12)
Il ltro cromatico va montato piano come in
gura ①, vericare che non sia inclinato.
► Se il ltro cromatico è inclinato o
comunque fuori posto ②, rischia di
cadere.
I ltri cromatici possono essere sovrapposti
nel portaltri, permettendo così di inserire
tutti i ltri necessari nché lo spessore
resta inferiore a 11 mm.
Installazione dei ltri cromatici
①
②
Loading...