Optika B-382PL-ALC, B-382PLi-ALC, B-382PH-ALC, B-383PH, B-382PHi-ALC Booklet [EN]

B-380 Series

OPTIKA

MICROSCOPES

®

ITALY

2

Laboratory

Middle-Level Routine Lab Upright Microscopes

2

Just What You Need. Right When Is Needed

ROUTINE IN UNIVERSITIES, LABS & INDUSTRIES

» Wide range to fullfil specific lab requirements
» Valuable solutions for life and material sciences
» Compliant with several observation methods

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THE PREFERRED PARTNER FOR ROUTINE TASKS

» Full planarity optics on 20 mm (N-PLAN) according to ISO 19012-1
» Fixed Koehler illumination for crisp and contrasted images
» Rounded edge, rackless stage to prevent scratches

Multiple Observation Methods

BRIGHTFIELD

DARKFIELD

FLUORESCENCE

METALLOGRAPHY

POLARIZATION

PHASE CONTRAST

104

100x Oil/Water Objective –

Only at OPTIKA

2

SAME OBJECTIVE FOR OIL AND WATER USE

» Oil represents the best media for high numerical aperture
» Water combines results with convenience
» Water is recommended especially for educational purposes

UNPARALLELED TIME & MONEY SAVING

» Save time by forgetting about tedious cleaning
» No time-wasting procedures for maintainance

No additional expenses due to inappropriate cleaning

»

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100x Water100x Water100x Oil100x Oil

105

105

2

X-LED

3

– Only Available at OPTIKA

STATE-OF-THE-ART ILLUMINATION SYSTEM

» Uncomparable light intensity, exclusive lens & collector design
» Constant pure-white colour temperature at all intensity levels

» Unmatched color fidelity, uniformity and brightness

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Halogen

Halogen

3

X-LED

X-LED

3

CUT ELECTRICITY BILLS BY 90%

» Money & energy saving, only 3.6 W
» More efficient brightness than a 50 W halogen lamp
» LED long lifetime (65,000 hours = 22 years at 8 hours/day usage)

3

X-LED

106

Multi-plug power supplyMulti-plug power supply

ALC

- Only Available At OPTIKA

2

AUTOMATIC LIGHT CONTROL IN 3 STEPS

» When another objective is used
» When the diphragm aperture changes
» When processing samples with different opacity

STEP 1

Set the brightness according
to your prefences.

FORGET ABOUT MANUAL LIGHT ADJUSTEMENT

» Choose the light intensity according to your preference
» Press the ALC button and the brightness is saved
» The microscope will automatically regulate the light

STEP 2

Press the ALC button to save
the brightness level.

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STEP 3

Forget about the illumination!

The microscope will automatically adjust the brightness for you, in case of:

• Another objective is used

• The diaphragm aperture is changed

• Another specimen with different opacity is processed

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B-380 Series

This series incorporates all the experience gathered by OPTIKA Microscopes in the field of light
microscopy, adapted specifically for common laboratory applications.
Suitable for routine microscopy with brightfield, darkfield (oil and dry), phase contrast,
fluorescence and polarized light, designed to be extremely stable on the bench and last long.

Purposely Designed For Intense Use, Effortless

Full of features that help being more comfortable especially in case of long-term use.
All the main controls are located close to each other to enable minimal movements and reinforce
the advantages that the ergonomy brings to this series.

X-LED3 Exclusive Lighting Source

Special technology able to double the light intensity for incomparable performance, ensuring
constant pure-white colour temperature (6,300K colour temperature).
Relevant money and energy saving thanks to the incredibly low energy consumptions which
allows you to cut the electricity bills by 90%!
The electric consumption (3.6 W only) pr
light intensity combined with low consumption.

oves the high efficiency of this system: incredibly high

20mm FN

18mm FN

ALC - Automatic Light Control, Only Available At OPTIKA

Incomparable Comfort With The Exclusive Automatic Light Control (ALC)

Light intensity is automatically adjusted by the microscope itself in order to maintain the same
level as the one the user has previously chosen.
No matter if the aperture of the diaphragm changes, if another objective is used, and if the
opacity of the sample is different...the microscope will set the light for you according to your
preferences.
On ALC Models.

Large Specimen View (20 mm Field Number)

The F.O.V. (field of view) is based on a comfortable
diameter of 20 mm.
This means that a wide area of the sample can
be inspected and allows a natural and easy view,
particularly needed in a laboratory environment.

Safe And Convenient Operations

Rounded edge rackless stage has been designed with a belt-driven
mechanism that allows a smooth movement without any protruding part.
This design gives you a more compact solution and lowers any risk of
injury after accidentally hitting the rack with your hands.

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Middle-Level Routine Lab Upright Microscopes

2

Universal Condenser For
Brightfield, Darkfield & Phase

OPTIKA B-380 phase contrast
microscopes are equipped with
a 5-position dedicated rotating
condenser for brightfield (standard
use), phase contrast (10x/20x, 40x
and 100x phase diaphragms), and a
darkfield position for dry objectives.

3

Exclusive X-LED

The special condenser with integrated, exclusive X-LED
illuminator replaces any other external and expensive lighting
source required for these applications and is ideal for
great-looking, rich and high-quality specimen view.

Darkfield Condenser

3

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In fluorescence we can offer several options.

According to your application and to the fluorochromes you
are using, we can help you to identify the best light source.

Traditional, HBO Fluorescence

» The most used and diffused method, worldwide
» Wide spectrum range for future upgrades

Innovative, LED Fluorescence

» Recommended for routine applications

» Cost-effective, money saving technology

» Ready for immediate operation

» Eliminate warm-up/cool-down times

» Forget lamp replacement & centering

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B-380 Series

10x - Darkfield10x - Darkfield

M-185
Darkfield condenser (dry)

With M-185 optional condenser you can easily obtain a darkfield view for dry
objectives.

M-335
Objective 50x/0.75
IOS W-PLAN MET

For applications where no cover slide
is required (such as sputum smear
analysis for tuberculosis diagnosis),
the M-335 objective provides excellent
results for stunning images.

110

50x - Fluorescence50x - Fluorescence

Blue filter

Green filter

Middle-Level Routine Lab Upright Microscopes

2

Get the most of our accessories

M-181
Complete Phase Contrast Set with IOS W-PLAN PH
obj. 10x, 20x, 40x, 100x, with Darkfield position

The B-380 series can be upgraded at any time with phase contrast kits (M-179
with W-PLAN PH objectives and M-181 with IOS W-PLAN PH objectives)
including all the components you need to inspect transparent specimens such
as microorganisms, thin tissue slices, lithographic patterns, fibers, glass, etc.

M-975.1 Ring with blue filter;

Increase the colour temperature of light (toward the blue).

M-977.1 Ring with green filter;

Optimize the resolution of phase contrast.

M-979.1 Ring with yellow filter;

Decrease the colour temperature of light (toward the red).

M-989.1 Ring with frosted glass filter;

Increase the uniformity of illumination, even further.

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Yellow filter Yellow filter

Frosted glass Frosted glass

M-156 Koehler field diaphragm

Additional field diaphragm for upgrading the Fixed
Koehler illumination system to a Full Koehler type.
To be ordered on newly purchased B-380 microscope.

M-975 Blue filter;

Increase the colour temperature of light (toward the blue).

M-977 Green filter;

Optimize the resolution of phase contrast.

M-979 Yellow filter;

Decrease the colour temperature of light (toward the red).

M-989 Frosted glass filter;

Increase the uniformity of illumination, even further.

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B-380 Series

Legend

1. Planachromatic Phase Contrast objectives.

2. Coded iris diaphragm for each objective.

3. B-383POL, tuff observed under polarized light.

4. Tilia three year stem at 4x magnification, B-383PL.

5. B-380 head with built-in Automatic Light Control system.

6. Head with Siedentopf adjustment system.

7. B-383POL attachment with Bertrand lens.

8. Handle for easy and comfortable transportation.

9. Coin at 4x magnification, B-383MET.

10. Coin at 50x magnification, B-383MET.

11. Innovative design of B-380 series.

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3

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4

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Middle-Level Routine Lab Upright Microscopes

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8

7

9

10

11

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2

B-380 Series - Brightfield Models

B-382PL-ALC

Brightfield binocular microscope with N-PLAN objectives, rackless

20

X-LED

A

N-PLAN

100x

WATER

33

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stage and combining the exclusive X-LED3 with ALC (Automatic
Light Control) for great-looking, rich and high-quality view.

Observation mode: Brightfield.
Head: Binocular, 30° inclined, 360° rotating (when ALC cable is unplugged).

Interpupillary distance: Adjustable between 48 and 75 mm.
Dioptric adjustment: On the left eyepiece tube.

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece: Quintuple revolving nosepiece, rotation on ball bearings.
Objectives:

N-PLAN 4x/0.10 N-PLAN 10x/0.25
N-PLAN 40x/0.65 N-PLAN 100x/1.25 (Oil/Water)
All with anti-fungus treatment.

Specimen stage: Double layer rackless mechanical stage, 150x139 mm,

75x33 mm X-Y range.

Focusing: Coaxial coarse (adjustable tension) and fine focusing mechanism

with limit stop to prevent the contact between objective and specimen.

Condenser: Abbe N.A. 1.25, with objective-coded iris diaphragm, focusable

and centerable.

Illumination (Fixed Koehler type): X-LED

and brightness control. ALC system.
Multi-plug 100-240Vac/6Vdc external power supply.

3

with white 3.6 W LED (6,300K)

20

X-LED

N-PLAN

100x

WATER

B-383PL

Brightfield trinocular microscope with N-PLAN objectives, rackless stage
and the exclusive X-LED3 for great-looking, rich and high-quality view.

Observation mode: Brightfield.

33

Head: Trinocular (fixed 50/50), 30° inclined, 360° rotating.

Interpupillary distance: Adjustable between 48 and 75 mm.
Dioptric adjustment: On the left eyepiece tube.

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece: Quintuple revolving nosepiece, rotation on ball bearings.
Objectives:

N-PLAN 4x/0.10 N-PLAN 10x/0.25
N-PLAN 40x/0.65 N-PLAN 100x/1.25 (Oil/Water)
All with anti-fungus treatment.

Specimen stage: Double layer rackless mechanical stage, 150x139 mm,

75x33 mm X-Y range.

Focusing: Coaxial coarse (adjustable tension) and fine focusing mechanism

with limit stop to prevent the contact between objective and specimen.

Condenser: Abbe N.A. 1.25, with objective-coded iris diaphragm, focusable

and centerable.

Illumination (Fixed Koehler type): X-LED

3

with white 3.6 W LED (6,300K)
with brightness control.
Multi-plug 100-240Vac/6Vdc external power supply.

114

B-380 Series - Brightfield Models

B-382PLi-ALC

Brightfield binocular microscope with IOS N-PLAN (Infinity
Corrected) objectives, rackless stage and combining the exclusive

20

X-LED

A

IOS

∞

N-PLAN

100x

WATER

33

3

X-LED

and high-quality view.

with ALC (Automatic Light Control) for great-looking, rich

Observation mode: Brightfield.
Head: Binocular, 30° inclined, 360° rotating (when ALC cable is unplugged).

Interpupillary distance: Adjustable between 48 and 75 mm.
Dioptric adjustment: On the left eyepiece tube.

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece: Quintuple revolving nosepiece, rotation on ball bearings.
Objectives:

IOS N-PLAN 4x/0.10 IOS N-PLAN 10x/0.25
IOS N-PLAN 40x/0.65 IOS N-PLAN 100x/1.25 (Oil/Water)
All with anti-fungus treatment.

Specimen stage: Double layer rackless mechanical stage, 233x147 mm,

78x54 mm X-Y range.

Focusing: Coaxial coarse (adjustable tension) and fine focusing mechanism

with limit stop to prevent the contact between objective and specimen.

Condenser: Abbe N.A. 1.25, with objective-coded iris diaphragm,

focusable and centerable.

Illumination (Fixed Koehler type): X-LED

(6,300K) and brightness control. ALC system.
Multi-plug 100-240Vac/6Vdc external power supply.

3

with white 3.6 W LED

2

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20

X-LED

IOS

∞

N-PLAN

100x

WATER

B-383PLi

Brightfield trinocular microscope with IOS N-PLAN (Infinity Corrected)
objectives, rackless stage and the exclusive X-LED3 for great-looking,
rich and high-quality view.

Observation mode: Brightfield.

33

Head: Trinocular (fixed 50/50), 30° inclined, 360° rotating.

Interpupillary distance: Adjustable between 48 and 75 mm.
Dioptric adjustment: On the left eyepiece tube.

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece: Quintuple revolving nosepiece, rotation on ball bearings.
Objectives:

IOS N-PLAN 4x/0.10 IOS N-PLAN 10x/0.25
IOS N-PLAN 40x/0.65 IOS N-PLAN 100x/1.25 (Oil/Water)
All with anti-fungus treatment.

Specimen stage: Double layer rackless mechanical stage, 233x147 mm,

78x54 mm X-Y range.

Focusing: Coaxial coarse (adjustable tension) and fine focusing mechanism

with limit stop to prevent the contact between objective and specimen.

Condenser: Abbe N.A. 1.25, with objective-coded iris diaphragm,

focusable and centerable.

Illumination (Fixed Koehler type): X-LED

(6,300K) with brightness control. Multi-plug 100-240Vac/6Vdc external
power supply.

3

with white 3.6 W LED

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2

B-380 Series - Phase Contrast Models

B-382PH-ALC

Phase contrast, darkfield and brightfield binocular microscope with
W-PLAN objectives, rackless stage and combining the exclusive X-LED3

20

X-LED

A

W-PLAN

33

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PHPH

DFDF

with ALC (Automatic Light Control) for great-looking, rich and high­quality view.

Observation mode: Brightfield, phase contrast and darkfield (dry).
Head: Binocular, 30° inclined, 360° rotating (when ALC cable is unplugged).

Interpupillary distance: Adjustable between 48 and 75 mm.
Dioptric adjustment: On the left eyepiece tube.

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece: Quintuple revolving nosepiece, rotation on ball bearings.
Objectives:

W-PLAN 4x/0.10 W-PLAN PH 10x/0.25
W-PLAN PH 40x/0.65 W-PLAN PH 100x/1.25 (Oil/Water)
All with anti-fungus treatment.

Specimen stage: Double layer rackless mechanical stage, 150x139 mm,

75x33 mm X-Y range.

Focusing:

with limit stop to prevent the contact between objective and specimen.

Condenser: Phase condenser (10x/20x, 40x, 100x) with darkfield (dry) and

brightfield.

Illumination (Fixed Koehler type): X-LED

and
Multi-plug 100-240Vac/6Vdc external power supply.

Coaxial coarse (adjustable tension) and fine focusing mechanism

brightness control.

ALC

system.

3

with white 3.6 W LED (6,300K)

20

X-LED

W-PLAN

PHPH

DFDF

B-383PH

Phase contrast, darkfield and brightfield trinocular microscope with
W-PLAN objectives, rackless stage and the exclusive X-LED3 for
great-looking, rich and high-quality view.

Observation mode: Brightfield, phase contrast and darkfield (dry).

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Head: Trinocular (fixed 50/50), 30° inclined, 360° rotating.

Interpupillary distance: Adjustable between 48 and 75 mm.
Dioptric adjustment: On the left eyepiece tube.

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece: Quintuple revolving nosepiece, rotation on ball bearings.
Objectives:

W-PLAN 4x/0.10 W-PLAN PH 10x/0.25
W-PLAN PH 40x/0.65 W-PLAN PH 100x/1.25 (Oil/Water)
All with anti-fungus treatment.

Specimen stage: Double layer rackless mechanical stage, 150x139 mm,

75x33 mm X-Y range.

Focusing:

with limit stop to prevent the contact between objective and specimen.

Condenser: Phase condenser (10x/20x, 40x, 100x) with darkfield (dry) and

brightfield.

Illumination (Fixed Koehler type): X-LED

with brightness control. Multi-plug 100-240Vac/6Vdc external power supply.

Coaxial coarse (adjustable tension) and fine focusing mechanism

3

with white 3.6 W LED (6,300K)

116

B-380 Series - Phase Contrast Models

B-382PHi-ALC

Phase contrast, darkfield and brightfield binocular microscope with

20

X-LED

A

IOS

∞

W-PLAN

PHPH

DFDF

33

IOS W-PLAN (Infinity Corrected) objectives, rackless stage and com­bining the exclusive X-LED3 with ALC (Automatic Light Control) for
great-looking, rich and high-quality view.

Observation mode: Brightfield, phase contrast and darkfield (dry).
Head: Binocular, 30° inclined, 360° rotating (when ALC cable is unplugged).

Interpupillary distance: Adjustable between 48 and 75 mm.
Dioptric adjustment: On the left eyepiece tube.

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece: Quintuple revolving nosepiece, rotation on ball bearings.
Objectives:

IOS W-PLAN PH 10x/0.25 IOS W-PLAN PH 20x/0.40
IOS W-PLAN PH 40x/0.65 IOS W-PLAN PH 100x/1.25 (Oil)
All with anti-fungus treatment.

Specimen stage:

78x54 mm X-Y range.

Focusing:

with limit stop to prevent the contact between objective and specimen.

Condenser: Phase condenser (10x/20x, 40x, 100x) with darkfield (dry) and

brightfield.

Illumination (Fixed Koehler type): X-LED

and brightness control. ALC
power supply.

Double layer rackless mechanical stage, 233x147 mm,

Coaxial coarse (adjustable tension) and fine focusing mechanism

system. Multi-plug 100-240Vac/6Vdc external

3

with white 3.6 W LED (6,300K)

2

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20

X-LED

IOS

∞

W-PLAN

PHPH

DFDF

B-383PHi

Phase contrast, darkfield and brightfield microscope with IOS W-PLAN
(Infinity Corrected) objectives, rackless stage and the exclusive X-LED3
for great-looking, rich and high-quality view.

Observation mode: Brightfield, phase contrast and darkfield (dry).

33

Head: (50/50), 30° inclined, 360° rotating.

Interpupillary distance: Adjustable between 48 and 75 mm.
Dioptric adjustment: On the left eyepiece tube.

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece: Quintuple revolving nosepiece, rotation on ball bearings.
Objectives:

IOS W-PLAN PH 10x/0.25 IOS W-PLAN PH 20x/0.40
IOS W-PLAN PH 40x/0.65 IOS W-PLAN PH 100x/1.25 (Oil)
All with anti-fungus treatment.

Specimen stage:

78x54 mm X-Y range.

Focusing:

with limit stop to prevent the contact between objective and specimen.

Condenser: Phase condenser (10x/20x, 40x, 100x) with darkfield (dry) and

brightfield.

Illumination (Fixed Koehler type): X-LED

with brightness control. Multi-plug 100-240Vac/6Vdc external power supply.

Double layer rackless mechanical stage, 233x147 mm,

Coaxial coarse (adjustable tension) and fine focusing mechanism

3

with white 3.6 W LED (6,300K)

117

2

B-383DK - Darkfield Microscope

Laboratory upright microscope for brightfield and darkfield observations with N-PLAN objectives (and W-PLAN 100x with iris) for biology and especially darkfield
fresh blood analysis and the exclusive X-LED
and expensive lighting source required for these applications and is ideal for great-looking, rich and high-quality specimen view. Our immersion darkfield system
provides the same result achieved by 150W external illuminators in combination with traditional cardioid darkfield condenser.

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X-LED

IOS

3

illumination system. The special condenser with integrated, exclusive X-LED3 illuminator replaces any other external

10x10x

∞

N-PLAN

DFDF

40x40x

Part Description

Observation mode: Brighteld, oil immersion darkeld.
Head: Trinocular (fixed 50/50), 30° inclined, 360° rotating.

Interpupillary
distance:

Dioptric adjustment:

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece
Objectives: N-PLAN 4x/0.10

Adjustable between 48 and 75 mm.

On the left eyepiece tube.

Quintuple revolving nosepiece, rotation on ball bearings.

N-PLAN 10x/0.25
N-PLAN 40x/0.65
W-PLAN 100x/1.25 (oil) with iris
All with anti-fungus treatment.

100x Brightfield100x Brightfield

100x Darkfield100x Darkfield

Part Description

Specimen stage: Double layer rackless mechanical stage, 150x139 mm,

75x33 mm X-Y range.

Focusing:

Brightfield
condenser:

Darkfieldfield
condenser:

Transmitted
illumination (Fixed
Koehler type):

Coaxial coarse (adjustable tension) and fine focusing
mechanism with limit stop to prevent the contact
between objective and specimen.

Abbe N.A. 1.25, with objective-coded iris diaphragm,
focusable and centerable.

Darkfield N.A. 1.36 (oil immersion) with built-in X-LED3.

X-LED3 with white 3.6 W LED (6,300K)
control

. Multi-plug 100-240Vac/6Vdc external power

supply.

with brightness

118

B-383FL - HBO Fluorescence Microscope

Laboratory upright microscope for brightfield and fluorescence observations with IOS N-PLAN objectives.
The HBO fluorescence illuminator provides an outstanding flexibility of use, standing the blue and green filter sets (supplied as standard) for Auramine, FITC, GFP
and YFP (with blue filter set) plus Rhodamine, Texas Red and TRITC (with the green one), yet giving the possibility to combine any other specific filter sets for future
upgrade. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.

20

2

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X-LED

IOS

∞

N-PLAN

100x

WATER

FL

33

Standard filterset

Name Excitation

lter (nm)

B Blue

G Green

460 – 490 505 515LP

510 – 550 570 575LP

10x - Blue excitation

10x - Green excitation

Dichroic mirror

cut-o (nm)

Emission

lter (nm)

Part Description

Observation mode: Brightfield, HBO fluorescence.
Epi-illumination and

filters:
Head: Trinocular (fixed 50/50), 30° inclined, 360° rotating.

Interpupillary distance:
Dioptric adjustment: On the left eyepiece tube.

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece:
Objectives: IOS N-PLAN 4x/0.10 IOS N-PLAN 10x/0.25

HBO 100 W high pressure mercury lamp.
3-position filter holder; blue and green included.

Adjustable between 48 and 75 mm.

Quintuple revolving nosepiece, rotation on ball bearings.

IOS N-PLAN 20x/0.40 IOS N-PLAN 40x/0.65
IOS N-PLAN 100x/1.25 (Oil/Water)
All with anti-fungus treatment.

Part Description

Specimen stage: Double layer rackless mechanical stage, 233x147 mm,

78x54 mm X-Y range.

Focusing:

Condenser:

Transmitted
illumination (Fixed
Koehler type):

Coaxial coarse (adjustable tension) and fine focusing
mechanism with limit stop to prevent the contact
between objective and specimen.

Abbe N.A. 1.25, with objective-coded iris diaphragm,
focusable and centerable.

X-LED3 with white 3.6 W LED (6,300K)
control

. Multi-plug 100-240Vac/6Vdc external power

supply.

with brightness

119

2

B-383LD - LED Fluorescence Microscope

Entry-level laboratory upright microscope for brightfield and fluorescence observations with IOS N-PLAN objectives. The extremely powerful LED fluorescence
illuminator is combined with blue excitation filter set for the visualization of the following fluorochromes: Acridine Yellow, Acridine Orange, Auramine, DiO, DTAF,
FITC, GFP, YFP, etc. LED fluorescence ensures unparalleled convenience eliminating warm-up/cool-down times and all the inconveniences related lamp replacement
and adjustment. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.

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X-LED

4x

IOS

∞

N-PLAN

100x

WATER

FL

10x

Part Description

Observation mode: Brightfield, LED fluorescence.
Epi-illumination and

filter:
Head: Trinocular (fixed 50/50), 30° inclined, 360° rotating.

Interpupillary distance:
Dioptric adjustment:

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece:

Objectives: IOS N-PLAN 4x/0.10 IOS N-PLAN 10x/0.25

High-power blue LED with brightness control.
3-position filter holder; blue included.

Adjustable between 48 and 75 mm.
On the left eyepiece tube.

Quintuple revolving nosepiece, rotation on ball bearings.

IOS N-PLAN 40x/0.65
IOS N-PLAN 100x/1.25 (Oil/Water)
All with anti-fungus treatment.

40x

Standard filterset

Name Excitation

lter (nm)

B Blue

Part Description

Specimen stage: Double layer rackless mechanical stage, 233x147 mm,

Focusing:

Condenser:

Transmitted
illumination
(Fixed Koehler type):

460 – 490 505 515LP

78x54 mm X-Y range.
Coaxial coarse (adjustable tension) and fine focusing

mechanism with limit stop to prevent the contact
between objective and specimen.

Abbe N.A. 1.25, with objective-coded iris diaphragm,
focusable and centerable.

X-LED3 with white 3.6 W LED (6,300K)
control

. Multi-plug 100-240Vac/6Vdc external power

supply.

Dichroic mirror

cut-o (nm)

with brightness

Emission

lter (nm)

120

B-383POL - Polarizing Microscope

Upright microscope for brightfield and polarizing light observations with strain-free IOS N-PLAN POL objectives. Complete of polarizer and analyzer filters,
Bertrand lens for conoscopic observation, compensator plates and high-precision rotatable stages. It comes with the exclusive X-LED3 illumination system to
deliver bright and clear images, along with all the accessories to perform accurate polarization analysis in biology and materials science.

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X-LED

4x with First Order plate

IOS

∞

N-PLAN

POLPOL

2

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Part Description

Observation mode: Brightfield, transmitted polarized light and conoscopy.
Bertrand lens

and polarizing
attachment:

Head: Trinocular (fixed 50/50), 30° inclined, 360° rotating.

Interpupillary distance:
Dioptric adjustment: On the left eyepiece tube.

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.

Nosepiece:

Swing-out type with centering mechanism for
observation in conoscopy/orthoscopy. Rotatable
analyzer from 0° to 90° with graduated scale.

Tint plates: 1° order red (λ); λ/4; Quartz wedge.

Adjustable between 48 and 75 mm.

One with crosshair.
Quadruple revolving nosepiece, rotation on ball bearings.
Centering system for each objective.

10x

10x with First Order plate

60x

Part Description

Objectives
(strain-free):

Specimen stage:

Focusing: Coaxial coarse (adjustable tension) and fine focusing

Condenser:

Transmitted illumination
(Fixed Koehler type):

IOS N-PLAN POL 4x/0.10 IOS N-PLAN POL 10x/0.25
IOS N-PLAN POL 40x/0.65 IOS N-PLAN POL 60x/0.80
All with anti-fungus treatment.

Rotatable stage with locking mechanism. Vernier scale with
accuracy 0.1 mm. Diameter 160 mm. Specimen slide clamps.

mechanism with limit stop to prevent the contact
between objective and specimen.

Abbe N.A. 1.25, with objective-coded iris diaphragm,
focusable and centerable. With rotating polarizing filter.

X-LED3 with white 3.6 W LED (6.300 K) with brightness
control. Multi-plug 100-240Vac/6Vdc external power supply.

121

2

B-383MET - Metallurgical Microscope

Brightfield upright microscope with IOS W-PLAN MET objectives and metallurgical attachment combining the exclusive X-LED3 lighting source both for incident
and transmitted illumination. The NCG (no cover glass) objectives are especially designed for microscopy use without a cover slip ideal for metallographic
samples and other opaque specimens.

20

Laboratory

33

X-LED

5x

IOS

∞

W-PLAN

MET

POLPOL

10x

20x

50x

Part Description

Observation mode: Brightfield, incident polarized light.
Epi-illumination and

polarizing filters:

Head: Trinocular (fixed 50/50), 30° inclined, 360° rotating.

Interpupillary
distance:

Dioptric adjustment: On the left eyepiece tube.

Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
Nosepiece: Quintuple revolving nosepiece, rotation on ball

122

3

X-LED

with white 3.6 W LED (6.300 K) with brightness
control. Field and aperture diaphragms, polarizer &
analyzer filters.

Adjustable between 48 and 75 mm.

bearings.

Part Description

Objectives
(strain-free):

Specimen stage: Double layer rackless mechanical stage, 233x147 mm,

Focusing:

Condenser:

Transmitted illumination
(Fixed Koehler type):

IOS W-PLAN MET 5x/0.12 IOS W-PLAN MET 10x/0.25
IOS W-PLAN MET 20x/0.40 IOS W-PLAN MET 50x/0.75
All with anti-fungus treatment.

78x54 mm X-Y range. With tempered glass plate.
Coaxial coarse (adjustable tension) and fine focusing

mechanism with limit stop to prevent the contact
between objective and specimen.

Abbe N.A. 1.25, with objective-coded iris diaphragm,
focusable and centerable.

X-LED3 with white 3.6 W LED (6.300 K) with brightness
control. Multi-plug 100-240Vac/6Vdc external power supply.

B-380 Series - Comparison chart

Model Head Eyepieces Nosepiece Objectives Stage Focusing Condenser Illumination

2

B-382PL-ALC

B-383PL

B-382PLi-ALC

B-383PLi

B-382PH-ALC

B-383PH

Binocular,
30° inclined

Trinocular,
30° inclined,
360° rotating

Binocular,
30° inclined

Trinocular,
30° inclined,
360° rotating

Binocular,
30° inclined

Trinocular,
30° inclined,
360° rotating

WF 10x/20 Quintuple,

reversed

WF 10x/20 Quintuple,

reversed

WF 10x/20 Quintuple,

reversed

WF 10x/20 Quintuple,

reversed

WF 10x/20 Quintuple,

reversed

WF 10x/20 Quintuple,

reversed

N-PLAN
4x, 10x, 40x,
100x (oil/
water)

N-PLAN
4x, 10x, 40x,
100x (oil/
water)

IOS N-PLAN
4x, 10x, 40x,
100x (oil/
water)

IOS N-PLAN
4x, 10x, 40x,
100x (oil/
water)

W-PLAN
4x, 10xPH,
40xPH,
100xPH (oil)

W-PLAN
4x, 10xPH,
40xPH,
100xPH (oil)

Rackless double
layer, 150x139mm,
moving range
75x33mm

Rackless double
layer, 150x139mm,
moving range
75x33mm

Rackless double
layer, 233x147mm,
moving range
78x54 mm

Rackless double
layer, 233x147mm,
moving range
78x54 mm

Rackless double
layer, 150x139mm,
moving range
75x33mm

Rackless double
layer, 150x139mm,
moving range
75x33mm

Coaxial coarse

and ne, limit

stop, adjustable
tension

Coaxial coarse

and ne, limit

stop, adjustable
tension

Coaxial coarse

and ne, limit

stop, adjustable
tension

Coaxial coarse

and ne, limit

stop, adjustable
tension

Coaxial coarse

and ne, limit

stop, adjustable
tension

Coaxial coarse

and ne, limit

stop, adjustable
tension

Abbe N.A. 1.25, iris
diaphragm, focusable
and centrable

Abbe N.A. 1.25, iris
diaphragm, focusable
and centrable

Abbe N.A. 1.25, iris
diaphragm, focusable
and centrable

Abbe N.A. 1.25, iris
diaphragm, focusable
and centrable

Phase condenser
(10x/20x, 40x, 100x)

with darkeld (dry) and
brighteld

Phase condenser
(10x/20x, 40x, 100x)

with darkeld (dry) and
brighteld

3.6 W X-LED3,
brightness control, ALC control.
Fixed Koehler

3.6 W X-LED3,
brightness control.
Fixed Koehler

3.6 W X-LED3,
brightness control, ALC control.
Fixed Koehler

3.6 W X-LED3,
brightness control.
Fixed Koehler

3.6 W X-LED3,
brightness control, ALC control.
Fixed Koehler

3.6 W X-LED3,
brightness control.
Fixed Koehler

Laboratory

B-382PHi-ALC

B-383PHi

B-383DK

B-383FL

B-383LD

B-383POL

Binocular,
30° inclined

Trinocular,
30° inclined,
360° rotating

Trinocular,
30° inclined,
360° rotating

Trinocular,
30° inclined,
360° rotating

Trinocular,
30° inclined,
360° rotating

Trinocular,
30° inclined,
360° rotating

WF 10x/20 Quintuple,

WF 10x/20 Quintuple,

WF 10x/20 Quintuple,

WF 10x/20 Quintuple,

WF 10x/20 Quintuple,

WF 10x/20
(one with
crosshair
reticle)

reversed

reversed

reversed

reversed

reversed

Quadruple,
reversed

IOS W-PLAN
10xPH,
20xPH,
40xPH,
100xPH (oil)

IOS W-PLAN
10xPH,
20xPH,
40xPH,
100xPH (oil)

N-PLAN
4x, 10x, 40x,
W-PLAN 100x
(oil, with iris
diaphragm)

IOS N-PLAN
4x, 10x, 20x,
40x, 100x
(oil/water)

IOS N-PLAN
4x, 10x, 40x,
100x (oil/
water)

Strain-free
IOS N-PLAN
POL 4x, 10x,
40x, 60x

Rackless double
layer, 233x147mm,
moving range
78x54 mm

Rackless double
layer, 233x147mm,
moving range
78x54 mm

Rackless double
layer, 150x139mm,
moving range
75x33mm

Rackless double
layer, 233x147mm,
moving range
78x54 mm

Rackless double
layer, 233x147mm,
moving range
78x54 mm

Round, 360°
rotating, 160mm
diameter, with
sample clips and
stop knob

Coaxial coarse

and ne, limit

stop, adjustable
tension

Coaxial coarse

and ne, limit

stop, adjustable
tension

Coaxial coarse

and ne, limit

stop, adjustable
tension

Coaxial coarse

and ne, limit

stop, adjustable
tension

Coaxial coarse

and ne, limit

stop, adjustable
tension

Coaxial coarse

and ne, limit

stop, adjustable
tension

Phase condenser
(10x/20x, 40x, 100x)

with darkeld (dry) and
brighteld

Phase condenser
(10x/20x, 40x, 100x)

with darkeld (dry) and
brighteld

Abbe N.A. 1.25, iris
diaphragm, focusable
and centrable. Additional
darkfield condenser,
N.A. 1.36, built-in X-LED

Abbe N.A. 1.25, iris
diaphragm, focusable
and centrable

Abbe N.A. 1.25, iris
diaphragm, focusable
and centrable

Abbe N.A. 1.25, iris
diaphragm, focusable
and centrable. With
rotating polarizer

3.6 W X-LED3,
brightness control, ALC control.
Fixed Koehler

3.6 W X-LED3,
brightness control.
Fixed Koehler

3.6 W X-LED3,
brightness control.
Fixed Koehler

3

Transmitted:

3.6 W X-LED3, brightness
control. Fixed Koehler
Incident: HBO 100 W high­pressure mercury bulb

Transmitted:

3.6 W X-LED3, brightness
control. Fixed Koehler
Incident: High-power blue
LED

3.6 W X-LED3,
brightness control.
Fixed Koehler

B-383MET

Trinocular,
30° inclined,
360° rotating

WF 10x/20 Quintuple,

reversed

IOS
W-PLAN
MET 5x, 10x,
20x, 50x

Rackless double
layer, 233x147mm,
moving range
78x54 mm

Coaxial coarse

and ne, limit

stop, adjustable
tension

Abbe N.A. 1.25, iris
diaphragm, focusable
and centrable

Transmitted and incident:

3.6 W X-LED3,
brightness control.
Fixed Koehler

123

2

B-380 Series -

Eyecups & Eyepieces

M-001 Huygens 5x eyepiece
M-008.1 WF10x/20 eyepiece, high eyepoint, with pointer, rubber cup
M-160 EW10x/20 eyepiece, high eyepoint, with rubber cup
M-161 EW15x/16 eyepiece, with rubber cup
M-162 WF20x/10 eyepiece
M-163 EW10x/20 micrometric eyepiece, high eyepoint, with rubber cup

Objectives

N-PLAN

Laboratory

M-164 N-PLAN objective 4x/0.10
M-165 N-PLAN objective 10x/0.25
M-166 N-PLAN objective 20x/0.40
M-167 N-PLAN objective 40x/0.65
M-168 N-PLAN objective 60x/0.85
M-169 N-PLAN objective 100x/1.25 (oil)

IOS N-PLAN

M-144 IOS N-PLAN objective 4x/0.10
M-145 IOS N-PLAN objective 10x/0.25
M-146 IOS N-PLAN objective 20x/0.40
M-147 IOS N-PLAN objective 40x/0.65
M-149 IOS N-PLAN objective 60x/0.80
M-148 IOS N-PLAN objective 100x/1.25 (oil)
M-144P IOS N-PLAN POL objective 4x/0.10
M-145P IOS N-PLAN POL objective 10x/0.25
M-146P IOS N-PLAN POL objective 20x/0.40
M-147P IOS N-PLAN POL objective 40x/0.65
M-149P IOS N-PLAN POL objective 60x/0.80
M-148P IOS N-PLAN POL objective 100x/1.25 (oil)

W-PLAN

M-059 W-PLAN objective 100x/1.25OI - (oil) objective with iris for DF
M-170 W-PLAN PH objective 10x/0.25
M-171 W-PLAN PH objective 20x/0.40
M-172 W-PLAN PH objective 40x/0.65
M-182 W-PLAN PH objective 100x/1.25 (oil)

IOS W-PLAN

M-634.1 IOS W-PLAN objective 50x/0.95 (oil)
M-336 IOS W-PLAN MET objective 5x/0.12
M-338 IOS W-PLAN MET objective 10x/0.25
M-339 IOS W-PLAN MET objective 20x/0.40
M-335 IOS W-PLAN MET objective 50x/0.75
M-698.2 IOS W-PLAN MET objective 100x/0.80 (dry)
M-1120.N IOS W-PLAN PH objective 10x/0.25
M-1121.N IOS W-PLAN PH objective 20x/0.40
M-1122.N IOS W-PLAN PH objective 40x/0.65
M-1123.N IOS W-PLAN PH objective 100x/1.25 (oil)

Accessories

124

M-179 - PH set - 10x, 40x, 100x W-PLAN PH obj. & BF/DF/PH condenser M-185 - Darkfield condenser for dry objectives

B-380 Series -

Stages

M-175 Rotating stage for polarising set (for 150x139mm rackless stage)
M-175.1 Rotating stage for polarising set (for 233x147mm rackless stage)
M-635-EU Heating stage (on newly purchased microscopes, for 233x147mm), EU
M-635-UK Heating stage (on newly purchased microscopes, for 233x147mm), UK
M-635-US Heating stage (on newly purchased microscopes, for 233x147mm), US
M-635-SW Heating stage (on newly purchased microscopes, for 233x147mm), CH
M-666.290-EU Applicable heating stage (for 150x139mm rackless stage), EU
M-666.290-UK Applicable heating stage (for 150x139mm rackless stage), UK
M-666.290-US Applicable heating stage (for 150x139mm rackless stage), US
M-666.290-CH Applicable heating stage (for 150x139mm rackless stage), CH

Condensers & Filters

M-174.1 Polarising set (filters only) (except for B-383POL)
M-179 PH set - 10x, 40x, 100x W-PLAN PH obj. & BF/DF/PH condenser
M-185 Darkfield condenser for dry objectives
M-975.1 Ring with blue filter, 45mm diameter
M-977.1 Ring with green filter, 45mm diameter
M-979.1 Ring with yellow filter, 45mm diameter
M-989.1 Ring with frosted glass filter, 45mm diameter

Camera Adapters

M-115 0.35x C-Mount projection lens
M-114 0.5x C-Mount projection lens
M-118 0.75x C-Mount projection lens
M-173 C-Mount projection lens for APS-C/full frame reflex cameras (trino)
M-620 0.35x focusable C-Mount adapter (biological microscopes)
M-620.1 0.5x focusable C-Mount adapter (biological microscopes)
M-620.2 0.65x focusable C-Mount adapter (biological microscopes)
M-620.3 1x focusable C-Mount adapter (biological & stereomicroscopes)
M-699 Universal adapter for C-Mount projection lens (trino)

Miscellaneous

15008 Immersion oil, 10ml
15009 Immersion oil, 100ml
15104 Cleaning kit
DC-002 Plastic dust cover, medium, 490(l)x490(h) mm (except for B-383POL, B-383MET, B-383LD1, B-383LD2 and B-383FL)
DC-003 TNT dust cover, medium, 600(l)x550(h) mm (only for B-383POL, B-383MET, B-383LD1, B-383LD2 and B-383FL)
M-005 Micrometric slide, 26x76mm, with 2 scales (1mm/100 & 10mm/100)
M-069 Solar charger
M-151 HBO 100W high-pressure mercury bulb for fluorescence (only for B-383FL)
M-151.1 HBO 100W high-pressure mercury bulb for fluorescence (OSRAM) (only for B-383FL)
M-156 Koehler field diaphragm (on newly purchased microscopes) (except for B-383POL)
M-1380 Centering telescope, 23mm diameter
VP-380 IQ/OQ/PQ manual for B-380 series

Accessories

2

Laboratory

How to connect the cameras to our microscopes.

Please refer to the Adapter reference list on Digital section.

15104 - Cleaning kit

It cleans glass quickly and effectively,
without leaving residue or odor.
Ideal for precision lens or prism cleaning.

M-069 - Solar charger

Included battery: rechargeable – Lithium-Poly.
Capacity: 2500 mAh. - Output voltage: 5 Vdc.
Autonomy: over 6 hours at medium intensity (X-LED³).
Charging models: with solar panel (12h),
with external USB power supply (2.5h)

125

2

Laboratory

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