Optika B-1000 POL Operation Manual
Version: 1
Issued: 25, 02, 2015
Model
B-1000POL
B-1000 Series
INSTRUCTION MANUAL
Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
List of accessories and spare parts
Overview
Unpacking
Assembling
Using the microscope
Maintenance
Troubleshooting
Equipment disposal
Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
Page 4
List of accessories and spare parts
CAT. NO. DESCRIPTION
M-1001 WF10x/22mm eyepiece (pair)
M-1002 WF10x/24mm eyepiece (pair)
M-1004.N Centering telescope, 30mm diameter
M-781 WF10x/22mm micrometer eyepiece (10mm, 0.1mm div.)
M-1011 Trinocular Head (3 positions)
M-1012 Binocular ERGO head
M-1033 Bertrand Lens with Analyzer and slot for slides (with Lambda, 1/4 Lambda and Quartz Edge)
M-1044 Quintuple nosepiece with centrable positions for POL objectives
M-1080 4x IOS POL PLAN objective
M-1081 10x IOS POL PLAN objective
M-1082 40x IOS POL PLAN objective
M-1083 60x IOS POL PLAN objective
M-1145 Rotating Stage, centrable
M-1146 Attachable mechanical stage for rotating Stage
M-005 Micrometric slide, 26x76mm, range 1mm, div. 0,01mm
M-690 Eyecup (pair)
M-619 Photo tube adapter for full frame SLR camera
M-173 Photo tube adapter for APS-C SLR camera
M-699 Photo tube adapter for DIGI digital camera series
M-620 CCD camera adapter for 1/3’’ sensor
M-620.1 CCD camera adapter for ½’’ sensor
M-114 CCD camera adapter 0,45x
M-113.1 Ring adapter, 30mm (for monocular and binocular microscopes)
M-617.1N Phase contrast set with IOS PLAN objectives 40x
Page 5
Overview
STAGE LOCK
KNOB
COARSE
FOCUSING
KNOB
NOSEPIECE
DIOPTRIC
ADJUSTMENT
RING
PHOTO PORT
MAIN ON/OFF
EYEPIECE
ANALYZER
FILTER
BERTRAND LENS
DISC
CONDENSER
CENTERING
KNOBS
APERTURE
DIAPHRAGM
POLARIZER
FIELD DIAPHRAGM
BRIGHTNESS
ADJUSTMENT KNOB
FOCUS-STOP KNOB
FINE FOCUSING
KNOB
OBJECTIVE
STAGE
FILTER
PLATE HOLDER
Page 6
Put the microscope stand on a
solid table.
First insert the Bertrand lens
attachment, use the 2mm
Allen wrench to tighten the
screw.
All screws are already inserted into each threaded hole.
(Fig.1)
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Assembling
Once you open the box, these are the microscope’s components:
① Condenser
② Optical head
③ Main body
④ Eyepieces
⑤ Power supply
⑥ Stage
⑦ Objectives
⑧ Bertrand lens and analyzer
⑨ Retardation plates
①
②
③
④
⑤
⑥
⑦
⑧ ⑨
Fig.1
Page 7
Insert the condenser under
the stage: position until it is
well inserted into its holder
(under the condenser there is
a pin that must fully enter the
guide of the holder). (Fig. 4)
AFTER INSERTING THE
CONDENSER, TIGHTEN
THIS SCREW
Insert the optical head above
the Bertrand lens, using the
other 3mm Allen wrench to
tighten the screw. (Fig.2)
Insert both eyepieces into
the tubes of the optical head.
(Fig.3)
SPRING
Mount the rotating stage: at
the bottom of the stage there
is a spring, push this spring
toward the stage support ①,
then push the stage
downward ②. (Fig.5)
Fig.2
Fig.3
Fig.4
Fig.5
①
②
Page 8
Insert the retardation plates
by sliding them into the slot
under the optical head. (Fig.7)
Center each objective using
the provided screwdrivers
(see chapter using the microscope). (Fig.8)
Insert the power supply jack
on the connector at the rear.
(Fig.9)
Screw each objective into the
thread of the nosepiece, in
order of magnication. (Fig.6)
Fig.6
Fig.7
Fig.8
Fig.9
Page 9
The rotating stage is provided
with:
a) Stage centering knobs;
b) Stage lock knob;
c) 45° rotation clicks;
d) Optional X-Y translator.
(Fig.10)
The Bertrand lens attachment
is provided with
a) Analyzer lter;
b) Retardation plates;
c) Lens focus adjustment;
d) Lens X-Y centering screws.
(Fig.11)
X-Y TRANSLATION KNOBS
STAGE CENTERING KNOBS
BERTRAND LENS ATTACHMENT
RETARDATION PLATES
Fig.10
Fig.11
Page 10
B-1000 illumination can be managed through
the keyboard placed on the left of the stand:
1) ON-OFF: press this button to turn on or off
the LED illuminator.
2) BOOST: press this button in order to incre-
ase the brightness (useful for high-magnication objectives or very opaque specimens).
2) AUTO-OFF: if you want the illuminator to
switch off automatically, press this button until
15, 30 or 60 minutes delay is set. After this
period of time, the light will turn off. You have
to press the ON-OFF button to turn it on again.
(Fig.12)
In order to activate the transmitted light illuminator, insert the plug of the external power
supply into the socket and turn on the main
switch on the side of the main body. Press
the ON-OFF button on the control keyboard
and turn the brightness adjustment knob to a
brightness suitable for observation. (Fig.13)
Do not enable boost mode while observing with
low magnication objectives (4x, 10x) with fully
open diaphgram: the high brightness may hurt
user’s eyes.
Control keyboard
LED settings
Preliminary settings
1) ON-OFF
3) AUTO-OFF
2) BOOST
MAIN
SWITCH
• The eld diaphragm is
fully open. (Fig.14)
• The aperture diaphragm
is fully open. (Fig.15)
• No lter is inserted under
the observation head.
Using the microscope
Fig.12
Fig.13
Fig.14
Fig.15
Before trying to focus the
sample, ensure that:
Page 11
Loosen the lock-screw, turn
the observation head to
a comfortable position for
observation, and then lock the
lock-screw. (Fig.18)
Hold the right and left parts of
the observation head using
both hands and adjust the
interpupillary distance by
turning the two parts until one
circle of light can be seen.
(Fig.20)
Adjust the observation
head
Adjust interpupillary
distance
• The analyzer lter is in
OUT position (lever fully
pulled out). (Fig.16)
• Photo port closed
(lever on observation
head fully in).
• Bertrand lens in OUT position (you read “0” on the
Bertrand lens disc under
the observation head).
• (If you use 4X objective)
Swing out lens of the
condeser in OUT position.
(Fig.17)
Fig.16
Fig.17
Fig.18
Fig.20
Page 12
Place the specimen on
the stage
Focus tension
adjustment
Focus-stop knob
Diopter adjustment
Fix the specimen slide on the
mechanical stage using the
slide-clamp. Ensure that the
specimen is centred over the
stage opening. (Fig.21)
Turn the tension-adjust knob
to get a suitable tension for
the focus system. (Fig.22)
NOTE: if the tension is too loose, the stage could go lower
by itself or the focus easily
lost after ne adjustment. In
this case, rotate the knob in
order to increase the friction.
Loosen the focus-stop knob,
rotate the coarse focusing
knob to bring the slide into
focus with a 4X or 10X objective. Then rotate the focus-stop
in order to block the height of
the stage. This simplies the
next focusing operations.
The focus-stop knob is also
useful to avoid accidental contacts between objective and
specimen. (Fig.23)
Adjust the ne focusing knob
to get the image sharp and
clear while observing with
your right eye, then turn the
left diopter ring to a sharp
and clear image also with the
other eye. The highpoint eyepieces allow the user to wear
glasses. (Fig.24)
NOTE :For the optimal parafocality of the image, it’s suggested to wear your glasses
during the normal use of the
microscope.
Fig.21
Fig.22
Fig.23
Fig.24
Page 13
Centering the
condenser
Centering the stage
Insert the swing-out lens of
the condenser ① and fully close the eld diaphragm ②. Rotate the condenser adjustment
knob ③ until you see a sharp
image of the closed eld
diaphragm (a bright spot of
light). Act on the condenser
centering screws ④until you
move the bright spot in the
center of the image eld. Then
re-open the eld diaphragm
②. (Fig.25)
With the Bertrand lens out,
focus on your slide. In this
procedure we’ll center the
optical axis of each objective
with the rotation axis of the
stage. Insert the 10x objective, rotating the nosepiece.
Look at your sample while
continuously rotating the
stage clockwise and then
counter-clockwise by a little
angle (e.g. 30° or 45°). During
these oscillations you should
spot a point on the sample
that doesn’t rotate on a circumference but only revolving
about itself (it is the center of
the rotations). (Fig.27)
Adjust the aperture of the iris
diaphragm under the conden-
ser to set the numerical aperture of the illuminator, thus
controlling image contrast and
resolution. It is suggested to
set the aperture diaphragm
between 70% and 80% of
objective’s N.A. (Fig.26)
①
③
④
②
A= FIXED POINT
(Center of rotation)
B= ROTATING POINT
Center of rotation on the optical axis.
Fig.25
APERTURE IRIS
DIAPHRAGM IMAGE
CENTERING THE CONDENSER
OBJECTIVE PUPIL
30-20%
70-80%
Fig.26
Fig.27
Page 14
Using the stage centering
screws, bring this point in
the center of the eld of view.
In this way the mechanical
center of rotation of the stage
coincides with the system’s
optical axis. (Fig.28)
Insert another objective, rotating the nosepiece. Repeat
the above operation (stage
rotations), and bring the point
into the center using the
objective centering screws
(not the stage centering
screws). (Fig.29)
NOTE :The stage can be
locked in its position using the
stage lock knob.
Bertrand lens
In order to have a conoscopic
view of your sample, you can
insert a Bertrand lens in the
optical path by rotating the
disc under the observation
head.
The Bertrand lens can be ne
aligned along Z axis by rotating this disc. (Fig.30)
The lens can also be centered
in X-Y axis by means of two
screws located at the back of
the lens. (Fig.31)
Fig.28
Fig.29
Fig.30
Fig.31
Page 15
Polarizer and analyzer
lters
Retardation lter plates
Insert or remove the lower
polarizer as required. It can
also be rotated at any angle.
(Fig.32)
The microscope is provided
with these retardation plates:
lambda, lambda/4 and quartz
wedge. You can insert them
in the optical path, sliding the
plate holder in the slot under
the observation head.
(Fig.34)
The analyzer lter can be
inserted by pushing the slider
inward. By rotating the front
disc, it can also be rotated at
any angle. (Fig.33)
Fig.32
Fig.33
Fig.34
Page 16
Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of
5°-40°C and a maximum relative humidity of 75 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no
moving parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn off the light immediately, cover the microscope with the included
dust-cover, and keep it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region
matches with the operation voltage of the equipment and that the lamp switch is in off-
position.
•
Users should observe all safety regulations of the region. The equipment has acquired
the CE safety label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not sufcient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or
near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.
Page 17
Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
1. Optical System
LED does not light. Power cord is unplugged. Plug power cord into the power outlet.
LED operates, but eld of view
remains dark.
Aperture and eld iris diaphragms are
not opened wide enough.
Adjust them to proper sizes.
Condenser is lowered too much. Adjust the condenser height position.
Light path selector knob is set to the
camera position.
Move the knob to the eye position.
Field of view is obscured or
not evenly illuminated
Light path selector knob is in an inter-
mediate position.
Set the knob according to the observation
method.
Revolving nosepiece is not correctly
engaged.
Make sure that the revolving nosepiece
clicks properly into place.
Condenser is not attached properly. Re-attach it.
Revolving nosepiece is not attached
properly.
Push the side dovetail all the way until it is
stopped.
An objective that falls outside of the
condenser’s illumination range is used.
Use a condenser to match the purpose.
Condenser is not properly centered. Center the condenser.
Field iris diaphragm is stopped down
too far.
Open the field iris diaphragm until it circumscribes the field.
Dirt or dust is visible in the
field of view.
Dirt/dust on the eyepieces Clean thoroughly.
Dirt or the to surface of the condenser
Dirt/dust on the specimen
Visibility is poor.
· Image is not poor.
· Contrast is poor.
· Details are indistinct.
· Image glares.
Condenser is lowered too far. Adjust the condenser height position.
Aperture iris diaphragm is stopped
down too far.
Open aperture iris diaphragm.
Revolving nosepiece is not mounted
properly.
Push the slide dovetail all the way until
it is stopped.
Front lens of objective is dirty. Clean objective.
Immersion oil is not being used with an
oil immersion objective.
Use immersion oil.
Immersion oil contains bubbles. Remove the bubbles.
Recommended immersion oil is not
used.
Use the provided immersion oil.
Dirt/dust on specimen. Clean it.
Dirt/dust on condenser
Inappropriate object side or cover
glass thickness.
Replace with glass of recommended thick-
ness.
Page 18
PROBLEM CAUSE SOLUTION
One side of image is blurred. Objective is not correctly engaged in light
path.
Make sure that revolving nosepiece
clicks into place correctly.
Revolving nosepiece is not correctly
mounted.
Push slide dovetail all the way until it
is stopped.
Stage is not correctly mounted. Re-attach it.
Specimen is not correctly mounted on
stage.
Place specimen correctly on to of
stage and secure it with slide holder.
Image appears to waver. Revolving nosepiece is not corrected
mounted.
Push slide dovetail all the way until it
is stopped.
Objective is not correctly engaged in light
path.
Make sure that revolving nosepiece
clicks into place correctly.
Condenser is not properly centered. Center the condenser.
Field of view becomes only
slightly brighter when the vol-
tage is raised.
Condenser is not properly centered. Center the condenser.
Condenser is lowered too far. Adjust the condenser height position.
2. Coarse/Fine
Adjustment
Coarse adjustment knob is
hard to turn.
Tension adjustment ring is tightened
excessively.
Loose ring.
You are trying to raise stage while focuslock lever is kept locked.
Unlock focus-look lever
Stage drifts down by itself or
focus is lost during observa-
tion.
Tension adjustment ring is too loose. Tighten ring.
Coarse adjustment will not go
all the way up.
Focus-look lever is locked at a too low
height.
Unlock focus-look lever.
Coarse adjustment will not go
all the way down.
Condenser holder is too low. Raise condenser holder.
Objective makes contact with
specimen before focus is
obtained.
Specimen is mounted upside down. Mount specimen correctly.
3. Observation Tube
Field of view of one eye does
not match that of the other.
Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment. Adjust diopter.
Different eyepieces are used on left and
right.
Change on eyepiece to match the other so
that both sides are the same type.
Your view is not accustomed to microscope observation.
Upon looking into eyepieces, try looking at
overall field before concentrating
on specimen range. You may also
find it helpful to look up and into distance
for a moment before looking back into
microscope.
4. Stage
Image shifts when you touch
stage.
Stage is not properly mounted. Clamp stage.
Specimen stops midway on
the Xaxis traverse.
Specimen is not correctly positioned. Place specimen correctly.
X- and Y-axis knobs are too
tight or too loose.
Tension of X- and Y-axis knobs is too high
or too low.
Adjust tension.
Page 19
Equipment disposal
Art.13 Dlsg 25 july 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating
to the reduction in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be
collected separately from other waste.
The separate collection of this equipment at the end of its lifetime is organized and managed by the producer.
The user will have to contact the manufacturer and follow the rules that he adopted for end-of-life equipment
collection.
The collection of the equipment for recycling, treatment and environmentally compatible disposal, helps to prevent
possible adverse effects on the environment and health and promotes reuse and/or recycling of materials of the
equipment.
Improper disposal of the product involves the application of administrative penalties as provided by the laws in force.
Versione: 1
Emesso il: 25, 02, 2015
Modello
B-1000POL
Serie B-1000
MANUALE D’ISTRUZIONI
Pagina 22
Sommario
Avvertenze
Simboli e convenzioni
Informazioni di sicurezza
Applicazioni d’uso
Lista accessori e ricambi
Panoramica
Disimballaggio
Assemblaggio
Uso del microscopio
Manutenzione
Risoluzione problemi
Misure ecologiche
Pagina 23
Avvertenze
Il presente microscopio è uno strumento scientico di precisione studiato per durare molti anni con una manutenzione minima, essendo costruito secondo i migliori standard ottici e meccanici e progettato per un
utilizzo quotidiano. Vi ricordiamo che il presente manuale contiene informazioni importanti sulla sicurezza e
manutenzione dello strumento, e deve quindi essere accessibile a chiunque lo utilizzi. Optika declina ogni
responsabilità derivante da un uso improprio dei suoi strumenti non indicato nella presente guida.
Simboli e convenzioni
Il seguente paragrafo è un glossario illustrato dei simboli usati nel manuale.
ATTENZIONE
Questo simbolo indica un potenziale rischio e vi avvisa di procedere con attenzione.
SHOCK ELETTRICO
Questo simbolo indica un rischio di shock elettrico.
Informazioni sulla Sicurezza
Precauzioni sicurezza elettrica
Prima di collegare il cavo di alimentazione alla presa di rete, assicurarsi che il voltaggio della rete elettrica del
vostro Paese sia compatibile con il voltaggio di alimentazione dello strumento, e che l’interruttore dell’illuminatore
sia sulla posizione spenta. L’utente deve osservare la regolamentazione riguardante la sicurezza in vigore nel
proprio Stato. Lo strumento è dotato di marcatura di sicurezza CE. In ogni caso, l’utente ha piena responsabilità
riguarda l’utilizzo sicuro dello strumento stesso. Prego fare attenzione alle seguenti linee guida, leggere il manuale per intero al ne di operare in sicurezza.
Applicazioni d’uso
Solo per applicazioni di ricerca ed usi didattici. Non indicato per utilizzo diagnostico e terapeutico
umano e veterinario.
Pagina 24
Lista accessori e ricambi
COD. DESCRIZIONE
M-1001 Oculari WF10x/22mm (coppia)
M-1002 Oculari WF10x/24mm (coppia)
M-1004.N Telescopio di centratura per contrasto di fase, 30mm di diametro
M-781 Oculare micrometrico WF10x/22mm (10mm, div.0,1mm)
M-1011 Testata trinoculare (3 posizioni)
M-1012 Testata binoculare ERGO
M-1033 Lente di Bertrand con Analizzatore e slitta per lamine (con Lambda, ¼ Lambda e cuneo di
quarzo)
M-1044 Revolver quintuplo con sistema di centratura per obiettivi POL
M-1080 Obiettivo PLAN IOS POL 4x
M-1081 Obiettivo PLAN IOS POL 10x
M-1082 Obiettivo PLAN IOS POL 40x
M-1083 Obiettivo PLAN IOS POL 60x
M-1145 Tavolino ruotante, centrabile
M-1146 Meccanismo traslatore per tavolino ruotante
M-005 Vetrino micrometrico, 26x76mm, range 1mm, div. 0,01mm
M-690 Paraocchi (coppia)
M-619 Adattatore fotografico per Reflex con sensore Full Frame
M-173 Adattatore fotografico per Reflex con sensore APS-C
M-699 Adattatore fotografico per fotocamera digitale DIGI
M-620 Adattatore per telecamere CCD con sensore 1/3”
M-620.1 Adattatore per telecamere CCD con sensore 1/2”
M-114 Adattatore per telecamera CCD 0,45x
M-113.1 Anello adattatore, 30mm (per microscopio monoculare e binoculare)
M-617.1N Set per contrasto di fase, Obiettivo PLAN IOS 40x
Pagina 25
Panoramica
MANOPOLA DI
BLOCCO TAVOLINO
MANOPOLA DI
MESSA A FUOCO
MACROMETRICA
REVOLVER
ANELLO
REGOLAZIONE
DIOTTRICA
USCITA FOTO/VIDEO
PULSANTE
ON/OFF
OCULARE
FILTRO
ANALIZZATORE
LENTE DI
BERTRAND
MANOPOLE
CENTRAGGIO
CONDENSATORE
DIAFRAMMA DI
APERTURA
POLARIZZATORE
DIAFRAMMA DI
CAMPO
MANOPOLA REGOLAZIONE
LUMINOSITA’
MANOPOLA BLOCCO
MESSA A FUOCO
MANOPOLA MESSA A
FUOCO MICROMETRICA
OBIETTIVO
TAVOLINO
ALLOGGIAMENTO
LAMINE DI RITARDO
Pagina 26
Posizionare lo stativo del
microscopio su un piano
stabile.
Per iniziare, inserire il dispositivo con la Lente di Bertrand,
utilizzando la chiave a brugola
2mm per stringere le viti.
Tutte le viti sono già posizionate ognuna nel relativo foro
lettato. (Fig.1)
Disimballaggio
Il microscopio è riposto in un imballo di polistirolo espanso. Rimuovere il nastro adesivo dal collo ed aprire la
parte superiore dell’imballo. Fare attenzione a non far cadere le parti ottiche (obiettivi e oculari) nell’estrarre il
microscopio dalla scatola per evitare che vengano danneggiati. Utilizzare entrambe le mani (una intorno allo
stativo e una alla base), slare il microscopio dal contenitore e appoggiarlo su un piano stabile.
Assemblaggio
All’apertura della scatola, i componenti del microscopio sono i seguenti:
①
②
③
④
⑤
⑥
⑦
⑧ ⑨
Fig.1
① Condensatore
② Testata ottica
③ Stativo
④ Oculari
⑤ Alimentatore
⑥ Tavolino
⑦ Obiettivi
⑧ Lente di Bertrand e
Analizzatore
⑨ Lamine di ritardo
Pagina 27
Inserire il condensatore sotto
il tavolino: controllare che
sia correttamente inserito
nel suo alloggiamento (sotto
il condensatore si trova uno
spinotto che deve entrare
completamente nella guida
dell’alloggiamento). (Fig. 4)
DOPO AVER INSERITO IL
CONDENSATORE,
STRINGERE QUESTA VITE
Inserire la testata ottica al di
sopra della lente di Bertrand,
usando la chiave a brugola
da 3mm per stringere le viti.
(Fig.2)
Inserire entrambi gli oculari
nei tubi portaoculari della
testata ottica. (Fig.3)
MOLLA
Montare il tavolino ruotante:
sotto il tavolino è posizionata
una molla, spingere questa
molla verso il supporto del
tavolino ①, poi spingere il
tavolino verso il basso ②.
(Fig.5)
Fig.2
Fig.3
Fig.4
Fig.5
①
②
Pagina 28
Inserire le lamine di ritardo
facendole scorrere all’interno
della slitta sotto la testata
ottica. (Fig.7)
Centrare ciascun obiettivo
usando i cacciaviti in dotazione (vedi paragrafo utilizzo
microscopio). (Fig.8)
Inserite la spina del cavo di
alimentazione alla presa sul
retro dello stativo. (Fig.9)
Avvitare ciascun obiettivo nel
foro lettato del revolver, in ordine di ingrandimento. (Fig.6)
Fig.6
Fig.7
Fig.8
Fig.9
Pagina 29
Il tavolino ruotante è dotato di:
a) Viti di centraggio tavolino;
b) Vite di ssaggio tavolino;
c) clickstop di rotazione di 45°;
d) movimento traslatore X-Y
opzionale. (Fig.10)
Il dispositivo con Lente di
Bertrand è dotato:
a) Filtro analizzatore;
b) Lamine di ritardo;
c) regolazione messa a fuoco
lenti;
d) Viti di centraggio lenti X-Y.
(Fig.11)
MANOPOLE DI TRASLAZIONE X-Y
VITI DI CENTRAGGIO TAVOLINO
DISPOSITIVO LENTE DI BERTRAND
LAMINE DI RITARDO
Fig.10
Fig.11
Pagina 30
L’illuminazione del B-1000 può essere controllata tramite tastiera posizionata sul lato
sinistro dello stativo:
1) ON-OFF: premere questo pulsante per
accendere/spegnere l’illuminatore LED.
2) BOOST: premere questo pulsante per
incrementare la luminosità (utile per obiettivi
ad elevati ingrandimenti e preparati molto
opachi).
2) AUTO-OFF: se desiderate che l’illuminatore
si spenga automaticamente, premete questo
pulsante no a impostare il tempo necessario
15, 30 o 60 minuti. Alla ne di questo periodo
di tempo, la luce si spegnerà. Dovrete premere il pulsante ON-OFF per accenderla nuovamente. (Fig.12)
Per attivare l’illuminatore in luce trasmessa,
inserire la spina dell’alimentatore esterno nella
presa di rete ed accendere l’interruttore principale sul lato dello stativo. Premere il pulsante
ON-OFF sulla tastiera laterale e ruotare la
manopola di regolazione della luminosità no
ad ottenere la luminosità adeguata all’osservazione. (Fig.13)
Non è possibile osservare in modalità BOOST
con obiettivi a bassi ingrandimenti (4x, 10x) e
con il diaframma di apertura completamente
aperto: l’elevata luminosità può danneggiare gli
occhi.
Tastiera di controllo
Regolazione LED
Impostazioni preliminari
1) ON-OFF
3) AUTO-OFF
2) BOOST
INTERRUTTORE
PRINCIPALE
• Il diaframma di campo sia
completamente aperto.
(Fig.14)
• Il diaframma di apertura
sia completamente aperto. (Fig.15)
• Nessun ltro deve essere
inserito sotto la testata di
osservazione.
Utilizzo del microscopio
Fig.12
Fig.13
Fig.14
Fig.15
Prima di tentare la messa a
fuoco del campione, assicu-
rarsi che:
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