Omni Mixer, Macro User Manual

Omni Mixer
& Macro
User Manual
OMNI MIXER
HOMOGENIZER
www.omni-inc.com
- READ ALL INSTRUCTIONS BEFORE USING.
- SAVE THIS OWNER’S MANUAL. The Omni Mixer and Omni Macro has been engineered for economical functionality as well as safety; however, basic safety precautions and common sense must always be demonstrated when using any electrical product. DO NOT attempt to modify any part of the Omni Mixer or Omni Macro. If you experience problems with or have questions about your Omni Mixer or Omni Macro, contact your authorized dealer or call Omni International at 800-776-4431 or 770-421-0058.
- DO NOT allow the machine to be submerged in any liquid.
- DO NOT use in any setting other than an indoor laboratory.
- DO NOT plug power cord into an incorrect outlet. To reduce the risk of burns, electrocution, re, or injury:
- Use this product only for its intended purpose as described in this booklet. DO NOT use attachments not recom­mended by the manufacturer.
- DO NOT operate the product if it is damaged in any way.
- Keep this product away from heated surfaces. WARNING: Keep all housings in place and in working order. DO NOT use the motor in a dangerous environment. DO NOT force the unit to do a job that it was not designed to do. NOTE: If you experience excessive vibration, heat, or bearing wear, please contact technical support. WARNING: DO NOT process pathogenic material in an open container, since aerosols created during normal pro­cessing could be inhaled by the operator. CAUTION: To prevent damage to the drive mechanism, never operate the motor without a sealed chamber assem­bly or generator probe installed. CAUTION: Never operate the motor with the generator probe (or sealed chamber assembly) partially attached to the coupling adapter. NOTE: If you experience excessive vibration, heat, or bearing wear, please contact technical support. WARNING: The Omni Mixer or Omni Macro must be operated with the chamber assembly resting on a table top or on the bottom of a cooling container to prevent rotating blades from being exposed if the chamber assembly becomes disengaged from the motor, and to minimize the possibility of a broken seal. CAUTION: To prevent damage to the drive mechanism, never operate the motor without a sealed chamber assem­bly or generator probe installed, or the ex rubber coupling removed from the motor. WARNING: DO NOT modify the plug or cord that is provided. Damaged or worn power cords should be repaired or replaced immediately by a qualied electrician. WARNING: Keep all housings in place and in working order. DO NOT use the motor in a dangerous environment. DO NOT force the unit to do a job it was not designed to do. WARNING: Reduce the risk of unintentional starting. Make sure that the switch is in the “OFF” position before plug­ging in the motor.
Should this product ever require service, please contact
Omni International at 1-800-776-4431
OMNI MIXER
HOMOGENIZER
www.omni-inc.com
Omni Mixer & Macro Homogenizers consist of the following:
Description Part Number (Mixer) Part Number (Macro) Quantity
Motor Drive Unit
17105 (115V) 17106 (220V)
17505 (115V) 17506 (220V)
1
Clamping Knob Assembly 17164 17164 1
Generator Probe Adapter 15001 15001 1
Finished Base Assembly 17234 17234 1
Support Post 17026 17026 1
Tool Kit 17104 17104 1
Instruction Manual 17258 17258 1
Overview
1
2
3
4
5
1. Support Post
2. Coupling Adapter
3. Support Base
4. Clamping Knob Assembly
5. Motor Support Housing
Description P/N QTY
Gen Probe Multi Tool 00-402 2
20, 30, 45, 55 mm Knife Tool 15100K 1
1/16” Allen Wrench 00-416 1
5/64” Allen Wrench 60027 1
3/32” Allen Wrench 60128 1
1/8” Allen Wrench 60241 1
5/32” Allen Wrench 737 1
5/16” x 5/8” Combo Wrench 60033 1
5mm Lower Bearing Press 59105 1
7mm Lower Bearing Press 59107 1
Lubricant 61971 1
The following components are supplied as part of
the Omni Mixer and Omni Macro tool kit (P/N 17104)
Operation
Assembly
1. Insert the support post into the support base. Secure the set screw through the back of the support base into the groove in the support post, using 1/8 in. Allen (P/N 60241) provided.
2. Place the clamping knob assembly in the motor support housing with the two at surfaces on the clamping mechanism facing the support post hole. The clamping knob assembly may be used either on the left or the right side of the Omni Mixer or Omni Macro.
3. Slide the motor support housing onto the support post and clamp it into place with the clamping knob assembly. The motor support housing should be positioned over the two longer legs of the support base.
The speed of the Omni Mixer or Omni Macro may be regulated to achieve optimum results when working with dierent materials. A speed selection knob is located at the side of the unit, as is a separate “OFF” or “ON” switch. When in the “ON” position the motor speed can be varied from 0 rpm to maximum speed. Select the optimal speed for each dierent combination of sample and accessory for best processing results.
Generator probes can be used in open containers or in sealed chamber assemblies. Sample process­ing eciency is eected by:
Amount of material processed vs. size and speed of the generator probe.
Container geometry and size (round vessels encourage swirling, while uted or cornered vessels disrupt ow patterns for more eective mixing/processing.
Processing speed vs. optimal speed.
Size and type of material and ow characteristics (material particles must be small enough to be carried into the generator head for optimal processing).
To operate the generator probe simply remove the blue protective cap from the end of the generator probe. Keep the blue protective cap on the tip of the generator probe when the generator probe is not being used.
NOTE: When using PTFE lower bearings, immerse the bottom of the generator probe in liquid or in the sample to avoid premature failure of the lower bearing.
NOTE: Liquid circulates through the two holes in the generator probe. DO NOT block the upper hole, although the lower hole may be completely submerged during processing.
NOTE: For optimal sample recovery during processing, completely remove the generator probe from the sample prior to turning o the motor drive unit.
To assemble the Omni Mixer or Omni Macro Homogenizer, refer to gure below:
Generator Probe Compatibility
Size
Processing
Range
Length Type
Resulting
Particle Size
Order No.
10 mm 1.5 mL - 100 mL
95 mm Saw Tooth Fine 15051
195 mm Saw Tooth Fine 150510
20 mm 50 mL - 2 L
100 mm Saw Tooth
Fine 15201
Coarse 15201W
145 mm Saw Tooth
Fine 15401
Coarse 15401W
195 mm Saw Tooth
Fine 15020
Coarse 15020W
30 mm 75 mL - 10 L 195 mm
Flat Bottom Fine 150-30NA-195
Flat Bottom
Wide Window
Coarse 150-30WA-195
45 mm
(Macro Only)
200 mL - 25 L 195 mm Flat Bottom Fine 150-45NA-195
55 mm
(Macro Only
300 mL - 30 L 195 mm Flat Bottom Fine 150-55NA-195
Generator Probe Installation
All generator probes (sold separately) are easily installed to the motor unit by following these steps:
1. Screw the generator probe adapter (P/N 15001) into the coupling adapter
2. Screw the generator probe into the generator probe adapter until the O-ring is completely inside the generator probe adapter.
3. If the generator probe does not thread fully into the generator probe adapter, then unscrew the generator probe from the generator probe adapter. Slightly rotate the rotor shaft collar on the generator probe. Then return to step 2.
4. Tighten the generator probe by hand with moderate force.
5. Remove the blue protective cap from the tip of the generator probe and the homogenizer is ready to operate.
NOTE: The tip of the generator probe, especially on the saw tooth generator probes, is sharp. For safety purposes it is advisable that the protective cap be replaced on the generator probe when not in use.
NOTE: The bottom of the generator probe is extremely fragile and care should be taken to protect it. Replace the blue protective cap on the end of the generator probe when the generator probe is not being used.
WARNING: Never operate the motor with the generator probe (or sealed chamber assembly) partially threaded onto the motor. To prevent damage to the motor and attachments be sure that the generator probe (or sealed chamber assembly) is fully threaded into the coupling adapter. Failure to do so will
result in damage to the coupling system.
Quick Connect Coupling Assembly
(Optional)
The Quick Connect Coupling Assembly is an optional accessory for the Omni Mixer and Macro.
To use, attach a Quick Connect Adapter to your chamber assembly or generator probe.
Next, slide the Adapter into the Quick Connect Coupling and a retaining ring positions into place for a secure hold.
To disconnect, simply slide the ring upward and the processing accessory is conveniently released.
Determining Motor Speed
To determine motor speed during operation, a tachometer (not in­cluded) may be inserted into the Omni Mixer or Omni Macro through the top of the motor as shown by removing the protective snap cap.
Vessel Restraint Disk
(Optional)
The Vessel Restraint Disk (P/N LT-750) can be used with the Omni Mixer and Macro to help reduce splashing and contain aerosols during homogenization. This accessory is compatible with 20mm and larger diameter rotor-stator generator probes and with containers up to six inches in diameter. The Vessel Restraint Disk also helps secure the container during processing.
Sealed Chamber Assemblies
Sealed chamber assemblies are available as separately ordered items for use with the Omni Mixer and Macro. They consist of chambers, many of which are uted to facilitate mixing by inhibiting swirling, gasket screw on cover seals to inhibit aerosol release, a shaft assembly, and a sharpened stainless steel blade, or generator probe.
Chamber materials include stainless steel, glass, polycarbonate, and polypropylene. A full range of titanium chamber assemblies is also available for applications requiring protection from leached elemental components, or where additional strength is a factor. Please contact your nearest Omni International representative, or Omni Interna­tional for complete details.
Using Blade Chamber Assemblies
Stainless Steel or Titanium - These chamber assemblies are intended for liquid and semi-sol­id materials. Homogenization and mixing will normally be completed within 30 seconds to one minute. For processing solid materials, rst reduce particle size diameter to 1cm or less. Load the material to be processed in the chamber. Do not ll the chamber to full capac­ity. Processing of too much volume could force liquid up through the rotor shaft.
Glass and Plastic - These chamber assemblies are intended primarily for low-viscosity liquids or for light duty homogenization at speeds below 5,000 rpm. At speeds greater than 5,000 rpm, place glass jar into a secondary container for added protection.
NOTE: The maximum recommended speed when using any of the blade chamber assem­blies is 10,000 rpm. Speeds in excess of 10,000 rpm can cause excessive bearing wear or part failure.
NOTE: Inspect glass or plastic chambers for any scratches or cracks, and do not use if any are found. Eye protection and utilization of a secondary container is recommended to prevent damage or injury in case of glass breakage.
Centrifugable - These assemblies are available in plastic or stainless steel, and should be used in the same manner as described for glass, plastic, and stainless steel above.
Breaking Down Specimen Particles - At speeds below 5,000 rpm, specimen particles may be broken down in the Omni Mixer by using hard
materials, such as glass beads. The Omni Mixer will act as a “bead mill”, agitating the chamber while the hard materials do the work. Since sharpness is not essential, a dull blade should be used.
Adding a Grinding Medium - To attain the desired eect (cell breakdown) a combination of procedures may be useful. First homogenize the specimen in solution at maximum recommended speed with a sharp knife blade. Then change to a dull knife blade, add a grinding medium and run the homogenizer at 5,000 rpm for ten to fteen minutes.
CAUTION: It is recommended that “bead mill” processing should not be undertaken in a glass vessel.
Using Generator Probes - To facilitate homogenization of some materials, it may be useful to pre-process the sample by using one of the techniques described above, and then completing the process by using a generator probe. Generator probes can achieve particle size reduction to less than 2 microns.
WARNING: When processing pathogenic mate­rial in a sealed container, carefully check seals and connections to be certain that they are not worn or leaking. A fume hood is recommended for processing pathogens, even with a sealed container. Our chambers are not aerosol sealed.
CAUTION: Knife blades are sharp. Handle care­fully to avoid injury.
Sealed Chamber Assemblies (cont.)
Sealed chamber generator probe assemblies are available for use with the Omni Mixer and Macro but are not supplied with the instrument. They consist of chambers, many of which are uted to facilitate mixing by inhibiting swirling, gasket screw on cover seals to inhibit aerosol release, and a generator assembly. Chamber materials include stainless steel, glass, polycarbonate, and polypropylene. A full range of titanium chamber assemblies is also available for applications requiring protection from leached elemental components, or where additional strength is a factor. Please contact Omni Interna­tional for complete details.
Sealed Generator Probe Assemblies
Installing Sealed
Chamber Assemblies
All chamber assemblies are easily installed to the motor unit by following these steps:
1. Attach the chamber (with adapter if required) to the cover/generator probe assembly. Method of attachment is a left-handed screw thread. Tighten the assembly securely.
2. Attach the chamber assembly to the motor by screw­ing the chamber cover into the coupling adapter.
3. If the chamber assembly does not thread fully into the coupling adapter, then unscrew the chamber assembly from the coupling adapter. Slightly rotate the rotor shaft collar on the generator probe. Then return to Step 2.
4. Before operating, make sure that the chamber assem­bly rests securely on the tabletop.
For safe operation, all sealed chamber
assemblies attached to the Omni Mixer or
Omni Macro must be lowered to the table
surface as shown in gure (right).
Positioning the
Chamber Assembly
At speeds above 5,000 rpm relatively high chamber temperatures may be caused from friction during processing. Immersion of the chamber in an ice bath or cooling chamber is recommended.
Using a Cooling Vessel
WARNING: Never operate the motor with the sealed chamber assembly (or generator probe) partially threaded onto the motor. To prevent damage to the motor and attachments be sure that the sealed chamber assembly (or generator probe) is fully threaded into the coupling adapter. Failure to do so will result in damage to the coupling system.
In the event that the motor drive connect and/ or the exible rubber coupling needs to be ser­viced or replaced, the aluminum coupling can be removed using the following procedure:
1. Unplug motor unit from the wall.
2. Insert at edge of combination wrench (included in motor tool kit) into the slots of the alumi­num coupling (see gure to right).
3. Using wrench for leverage, loosen aluminum cou­pling by twisting it counter-clockwise.
Installation of the coupling is the reverse of these steps.
Maintenance
Motor Drive Unit Removal of
Aluminum Coupling
The motor drive housing can be cleaned period­ically with a mild detergent. Never use solvents to clean the unit or accessories. While resistant to most laboratory chemicals, care should be tak­en to wipe o any concentrated and potentially damaging liquids.
Once per month the aluminum coupling adapter (see gure to right) of the homogenizer should be removed and cleaned. After cleaning, a good lubricant should be applied to the threads.
To a large measure, how well your homogenizer performs its job is determined by the condition of the carbon contact brushes (P/N 61810). If brushes require replacement, the unit may run at a reduced speed and make unusual noises, or it may run sporadically and lose power. If this occurs with your Omni Mixer or Omni Macro, unplug the cord, loosen the brush caps one at a time and check each brush. If the brushes are less than 1/8” long and the ends of the brushes (which contact the commutator) are rough and/ or pitted, the brushes no longer make adequate contact with the commutator and should be re­placed (check both brushes since both brushes generally do not wear at the same rate). If one brush is worn replace both brushes. Make sure that the brushes are installed properly. The brushes have a curved surface at the end which must match the curvature of the commutator. The curvature of the commutator is the same as the curvature of the motor housing.
If the brushes are not installed properly they will not contact the commutator and the motor will not run. After replacing the brushes, make sure to remove the exible rubber coupling, place the motor on a clean surface, and run it freely (no­load, without a generator or chamber assembly attached) for 15 to 30 minutes before using the unit to process samples. This will allow the brushes to “seat” properly and will extend the life of each set of brushes. This will also extend the total life of your motor since the commutator surface will wear evenly.
Motor Brush Maintenance
Motors are equipped with sealed ball bearings. Under normal use they require no additional lu­brication. A tube of lubricant (P/N 61971) is in­cluded with every drive unit and should be used to lubricate the threads of all connecting thread­ed parts, as well as the drive pin at the tip of the oating shaft .Lubricant should not be used to lubricate moving parts or bearings.
Bearings and Lubrication
Grounding Instructions
In the event of a malfunction or breakdown, grounding provides a path of least resistance for electric current to reduce the risk of electric shock. This instrument is equipped with an elec­tric cord which is grounded to the chassis hous­ing. The plug must be plugged in to an outlet that is properly installed and grounded in accor­dance with all local codes and ordinances.
Troubleshooting
Do not attempt to service the Omni Mixer or Macro in a manner other than those dis­cussed in this manual. For any issue that is unsuccessfully corrected using this guide, please contact your authorized dealer or call technical assistance at Omni International at 1-800-776-4431 or 770-421-0058.
PROBLEM CORRECTIVE ACTION
The Omni Mixer/Macro is plugged in and turned on but is not functioning.
- Check power cord connectors
- Check wall socket for power to the outlet
Motor is turned ON and makes a “buzz­ing” sound, but is not working.
- Brushes may need to be replaced. Contact Omni International or distributor.
Motor unit operating speed declines, stalls intermittently, or stops completely
- Brushes may need to be replaced. Contact Omni International or distributor.
PTFE bearing wears quickly
- Fluid level may be too low in the tube.
- Immerse the probe deeper into the uid.
Excessive splashing in sample tube - Fluid level too low for tube size
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PN: 17258 Rev.B
935C Cobb Place Blvd. Kennesaw, GA 30144
800.776.4431 • 770.421.0058 www.omni-inc.com
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