Omega TCY 60, TCY 90 User guide

User’s Guide

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TCY 60 & TCY 90

Thermal Cyclers

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For immediate technical or application assistance:

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®

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Irlam, Manchester M44 5BD England Toll-Free: 0800-488-488 TEL: +44 (0)161 777-6611 FAX: +44 (0)161 777-6622 e-mail: [email protected]

It is the policy of OMEGA Engineering, Inc. to comply with all worldwide safety and EMC/EMI regulations that apply. OMEGA is constantly pursuing certification of its products to the European New Appr

oach Directives. OMEGA will add the CE mark to every appropriate device upon certification.

The information contained in this document is believed to be correct, but OMEGA accepts no liability for any errors it contains, and reserves the right to alter specifications without notice. WARNING: These products are not designed for use in, and should not be used for, human applications.

Contents

1. Introduction........................................ .... ........ .... .... .... ........ ... .... .... ........ .... .... .... ......4

2. Safety and installation........................................... .... .... .... ....... .... .... .... .... ........ .... ..5

3. Before switching on....................................................... .........................................7

4. TCY specification....................................................... .... ........ .... ... .... ........ .... .... .... ..8

5. User operation and consumables.........................................................................9

Heated lid ......................................................................................................................................... 9

Tubes or reaction vessels............................................................................................................... 10

0.5ml Tubes.................................................................................................................................... 10

Switching on................................................................................................................................... 10

6. Front panel controls.............................................................................................12

The LCD Display ............................................................................................................................ 12

Indicators........................................................................................................................................ 12

The keys......................................................................................................................................... 13

7. Programming the TCY..........................................................................................14

Programming Options..................................................................................................................... 15

Option 1: Creating programs using templates................................................................................. 15

Option 2: Creating a new program.................................................................................................. 19

Example programs ......................................................................................................................... 20

Programming.................................................................................................................................. 20

To program increment or decrement temperature or time. ............................................................. 24

To run a program............................................................................................................................ 27

8. Information ......................... .... .... ........ .... .... .... ........ .... .... ........ .... .... ... ........ .... .... ....29

9. Configuration ............................................................. .... .... ....... .... .... .... ........ .... ....30

10. Notes on transferring protocols from other thermal TCYs............................32

11. User maintenance............................................................................... ................33

Cleaning your TCY ......................................................................................................................... 33

Heater Block removal ..................................................................................................................... 33

12. Some frequently asked questions ....................................................................34

13. Additional information .......................................................................................35

Fault Finding................................................................................................................................... 35

Fuses ............................................................................................................................................. 35

The heated lid over-temperature cut-out.........................................................................................35

Insulation testing ............................................................................................................................ 35

Interchangeable blocks................................................................................................................... 35

3

1. Introduction

The TCY provides the researcher with the means of accurately controlling the temperature profile of samples. It has many applications, including DNA amplification cycle samples between 4°C and 99°C.

The TCY is programmed by means of an integral keypad and LCD display. A program, which can be recalled from memory, consists of:

 a series of specified temperatures in °C  the times for which each specified temperature will be held (Hold Times)  the desired heating or cooling rates, in °C/sec, between each specified temperature  optional instructions as to whether the times and/or the temperatures are to increase or

decrease when a cycle is repeated.

The memory can store up to 37 programs.

Before using the TCY make sure you have read this manual carefully.

1

and sequencing. The TCY can

1

Aspects of the PCR process are claimed in USA Patent Nos. 4,683,195; 4,683,202, and 4,965,188. Use of the TCY in such

processes does not convey a licence to practice the processes themselves.

4

2. Safety and installation

Operator Safety

Please read this manual carefully before using the TCY. If the equipment is not used in the manner described in this manual the protection provided by the equipment might be impaired.

This instrument is designed to conform to international safety requirements and are fitted with an over-temperature cut-out. If a safety problem should be encountered then switch off the unit at the power socket and remove the plug from the electricity supply.

Warning

HIGH TEMPERATURES ARE DANGEROUS as they can cause serious burns to operators and ignite combustible material. Users should be aware of the following potential hazards:

 USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS

 DO NOT use combustible substances near hot objects

 DO NOT operate the instrument in the vicinity of inflammable liquids or gases

 DO NOT place any liquid directly into the instrument, always use the appropriate

plastic consumables

After heating samples, remember that parts of the instrument, namely the lid and block and any associated accessories may be very hot.

Unpacking

When unpacking please ensure that the following have been removed from the packaging:

 OMEGA TCY

 Power cable

 Decontamination certificate

The user is advised to keep the original packaging in case the instrument ever needs to be returned for service or repair. Omega Engineering accepts no responsibility for damage incurred unless the unit is correctly packed and transported in its original packaging.

Please note that the screen of the thermal TCY is easily damaged by sharp objects such as pens, pencils and fingernails. This type of damage will be considered as misuse and invalidate the guarantee for this component.

Installation

The instrument should be carried using both hands. Never move or carry the instrument when in use or connected to the power electricity supply.

1. The TCY includes a plug-in power cable.

2. Before connecting the instrument to the power supply, check the voltage against the rating plate (located on the back of the unit). Ensure that the voltage selector switch (located above the On/Off switch) is set to the appropriate voltage for the local supply. Please note that the unit must be grounded to ensure proper electrical safety. Connect the power cable to a suitable plug according to the table below.

Connection 220/240V, 50/60Hz Supply 110V/120V Supply, 50/60Hz

Live Brown Black

Neutral Blue White

Earth Green/yellow Green

5

3. UK ONLY: The fused plug supplied with the power cable is fitted with a 10 Amp fuse to protect the instrument and the user.

4. Units showing 230V, 50/60Hz on the rating plate also operate between 210 and 260V; units with 120V, 50/60Hz also operate between 100 and 130V and units marked with 100V will operate between 90 and 110V. In all cases the heating rate will degrade by approximately 8% at the extremes of the voltage range.

5. Plug the power cable into the socket on the back of the instrument.

6. Place the unit on a suitable flat bench or in a fume cupboard if required, ensuring that the air inlet vents on the underside are free from obstruction. Position the instrument with a minimum distance all round of 200mm from walls or other items and between each unit if multiple units are being used to permit efficient air flow for each instrument.

7. Switch on the instrument:

I Power Switch On

O Power Switch Off

Replacement cable

Should the power lead need replacement, a cable of 1mm

2

of harmonized code H05VV-F

connected to an IEC320 plug should be used. IF IN DOUBT CONSULT A QUALIFIED

ELECTRICIAN.

The TCY is designed operate under the following conditions:

 Indoor use  Ambient temperature range +5

o

C to +40oC

 Altitude to 2000m  Relative humidity not exceeding 95%  Power supply fluctuations not exceeding 10%  Over voltage category II IEC 60364-4-443  Pollution degree 2

o

Note: The control specifications are quoted at an ambient temperature of 20 may deteriorate outside an ambient temperature of 10

o

C to 30oC.

C. The specification

The instrument has been tested for radio frequency interference and is certified under EN61326.

6

3. Before switching on

Ensure that the appropriate fuses are fitted for your supply:

 For 230V set the voltage selector switch

set to 230V and fit T6.3A fuses.

 For 115V set th e switch to 115V and fit

T8A fuses.

 For 110V the switch will be set to 115V

T8A fuses will be fitted.

On/Off switch

7

4. TCY Specification

Temperature

Temperature range 4°C to 99°C Temperature set point precision 0.1°C Block uniformity (over full range) ± 0.5°C Temperature accuracy ± 0.5°C End of program cooling below ambient Yes

Heating/Cooling Rate

Heating rate, between 55°C and 90°C on a 50°C to 95°C step: 48 x 0.2 ml micro tubes 3.5°C/sec 30 x 0.5 ml micro tubes 3.5°C/sec

Cooling rate, between 90°C and 55°C on a 95°C to 50°C step:

48 x 0.2 ml micro tubes 2.5°C/sec

30 x 0.5 ml micro tubes 2.5°C/sec

Heated Lid

Heated lid enable/disable Yes Selectable heated lid temperature 100°C to 115°C Over-temperature cut-out Fixed at 145°C Temperature sensor Thermistor Heater type Etched foil Heater power 132W Warm up time 4 min approximately

(ambient to 105°C)

The heated lid will only come on if the set temperature is above 35°C

Programming

Program naming using alpha keys Yes Program password protection Yes Number of programs (3 step) 37 Maximum number of steps per stage 35 Maximum number of stages per program 25 Minimum hold time 1 sec Maximum hold time 18 hrs Incremental/decremental temperature Yes Incremental/decremental step hold time Yes Programmable ramp rate (heat or cool) Variable in 0.1°C/sec steps Programmable ramp rate resolution 0.1°C

Running Programs

Pause button Yes Stop button Yes End of program alarm Yes Auto restart on power fail Yes

Interconnectivity

Dimensions

Height 260mm Width 220mm Length 420mm

8

Serial Port

Power

Power consumption 500W

The units are voltage selectable by a slide switch. When the unit is set to 115V it will work at any voltage between 110V and 130V. When the unit is set to 230V it will work at any voltage between 198V and 252V.

Units marked 100V will work at any voltage between 90 and 110V. The performance will vary and will not necessarily meet the above typical

specification at the extremes of voltage.

5. User operation and consumables

Heated lid

To release the heated lid, lift the back of the handle as show and use it to lift the lid. To close the lid, lower the lid onto the block containing the samples and press the handle down to shut.

close the lid without tubes being fitted in the block.

The heated lid has a rotating knob to adjust the lid pressure on the samples, allowing for a variety of consumables to be used to adjust the pressure:

 Place the samples in the block and place the lid in the

down position.

 Adjust the orange rotating knob anticlockwise to give

the least pressure then close the lid fully.

 Gently rotate the knob clockwise until you can feel

pressure beginning to be applied.

 Rotate the knob a further quarter of a turn and this will

give the correct pressure.

1.

Do not

Do not over-tighten the knob as this will cause deformation of the silicone rubber membrane (where use) and reduce the effectiveness of the lid. Once the pressure has been set it does not need to be adjusted unless a different block or consumable is used.

9

Tubes or reaction vessels

Omega Engineering does not recommend any specific tube or reaction vessel other than those described in this manual. We recommend using reaction volumes between 20 and 200µl. The tubes must withstand a pressure of 1 atmosphere at 100°C. Any vessel must be able to withstand the temperatures you are using without any danger of them deforming to the point where they fracture.

To test your tubes, put 25µl of water into each of 5 tubes and subject them to a typical thermal cycling protocol. At the end of the program, measure the volume remaining using a micropipette. A loss of more than 1 or 2µl indicates a vapour leak.

The amount of volume loss you observe and the change in reactant concentrations you can tolerate determine the minimum volume that can be used. Typical volume losses of 1µl in 30 cycles allow the use of samples of 20µl or less.

During the final cool-down, a ring of condensation may form above the liquid level but below the top of the sample block. This is normal and does not effect the reaction.

0.5ml Tubes

The heated lid mechanism of the TCY with the 0.5ml block is optimised for flat top tubes. In order to fit the maximum number of tubes in the block, they need to be placed so that all the hinges are in the same relative orientation. An example is illustrated below.

Place the tubes in the block in the same orientation in order to achieve the maximum capacity.

Always make sure the pressure on the heated lid is balanced by spreading the tubes across the block or inserting some dummy tubes if necessary.

10

Switching on

x

]

Omega

Version X.XX

Block Type 96

Block 25x0.2ml

TC 3000

TCY

0.2ml

or 60x0.5ml

When you switch on the unit a screen similar to the one shown here will appear briefly. This indicates the software version and block type installed in the unit.

Programs [0]

Methods [0]

Information

Information Configuration

Configuration [ , to select

[ , to select ]

When the initial screen has disappeared the screen shown here will automatically appear. There are three options:

1. ‘Programs’ for creating and running programs;

2. ‘Information’ for obtaining information about the unit e.g. serial number;

3. ‘Configuration’ for setting up program defaults and settings.

Each of these is explained in detail in section 6.

11

6. Front panel controls

M

Operation or Program lines

Prompt line

Down Up Decimal Point

& Insert

End Delete

Alpha/Numeric keys Status Pause

Power Enter

The LCD Display

Programs [0]

ethods [0]

Information

Information Configuration

Configuration [ , to select ]

[ , to select ]

-- Stage 1 ----------

-- Stage 1 ---------Number cycles 10

Number cycles 10

Step 94.0ºC 00m30 max

[‘.’ to insert step ]

[‘.’ to insert step ] [ , to edit ]

[ , to edit ]

[ , to edit ] [ to delete step ]

[ to delete step ]

Indicators

Power: This indicator is lit when there is power to the TCY.

Status: This indicator is lit when the TCY is running a program. It is off when

the unit is stopped. It will flash and beep slowly when the unit is paused. It will flash after a five second quick sequence of beeps when the unit reaches its final hold temperature.

The TCY has a four line LCD display. The top three lines are information or program lines, while the bottom line is a prompt line. The active line flashe s on screen and is shown in bold in this manual.

The bottom line may change or flash between two or three different prompts depending upon which program line is active. An example of a prompt line which flashes between three different prompts is shown here.

12

The keys

The End, Finish or Exit key

The Up arrow key

The Down arrow key

The Decimal Point and Insert key

The Delete key

The Pause key

The Enter or Accept key

The Alpha/Numeric keys

The “End” or “Finish” key is used to finish editing programs or to exit a program. It is also used to exit a sequence of key operations.

The “Up arrow” key is used for scrolling up through the lines on the screen. It is also used to increase certain fields when editing.

The “Down arrow” key is used for scrolling down through the lines on the screen. It is also used to decrease certain fields when editing.

The “Decimal Point” key is used when editing numeric fields. It is also used as an Insert key during program editing to insert additional steps and other field sets.

The “Delete” key is used during program editing to delete steps and stages.

The “Pause” key is used during program editing to insert a pause after a stage or to insert increment and decrement times and temperatures. It is also used to pause a program when it is running.

The “Enter” key is used to enter an operation or the edit mode. It is also used to ‘Accept’ a field or program change.

The “Alpha/N umeric” keys are used to enter numbers and letters during programming. If it is a numeric field then numbers will be inserted; if it is an alpha field then letters will be inserted. Each key will cycle through the letters shown on the key (0 will insert a space). To insert a number, press and hold the appropriate key.

13

7. Programming the TCY

The table below illustrates the symbols which appear on the LCD display and the relevant keypad button to which they refer.

The following terms are used when programming the TCY:

Stage

Cycle Step

Programs [0]

Methods [0]

Information

Information Configuration

Configuration [ , to select ]

[ , to select ]

* * New Methods ** []

* * New program ** []

2 STEP TEMPLATE 501

2 STEP 3 STEP TEMPLATE 502

3 STEP [ , to select ]

[ , to select ]

In the prompt line is equivalent to the “End” key.

On a program line means the program is locked; it can be copied and then edited but not edited directly.

Up arrow

Down arrow

Enter

Pause

A ‘Stage’ is the name given to the main repeated part of a program containing the ‘Steps’ to be repeated. A program can contain a maximum of 25 stages although typically only one or two are used.

A ‘Cycle’ is the number of repetitions of a ‘Stage’ A ‘Step’ is a user defined program line containing a temperature, a hold

time and ramp rate. Typically three steps are created within one ‘Stage’.

To enter the programming section of the TCY Ⅴ software, highlight ‘methods’ using the “Up Arrow” or “Down Arrow” keys

Press “Enter”

This screen lists all the pr ograms saved on the unit. Use the “Up Arrow” and “Down Arrow” keys to scroll through the list.

The number next to the program name is the unique “Speed dial” number allocated to the program for rapid retrieval (see below).

14

Programming Options

[2]

C

9

C

7

C

9

C

7

3

H

P

-------

-

In d

9

H

S

N

S

º

C 0

S

C 0

S

º

C 0

-------

-

F

3

H

P

-------

-

In d

9

H

S

N

S

º

C 0

S

C 0

S

º

C 0

-------

-

Post

run7

P

There are two options available for creating programs on the TCY :

1. Copy and edit one of the pre-written templates.

2. Create a new program from the defaults.

Option 1: Creating programs using templates

There are two pre-written basic PCR templates: a two-step and a three-step template. These can be copied and modified as required. "In denat" stands for Initial Denaturing.

2 STEP TEMPLATE

Heated Lid 105 º

Heated Lid 105 º Preheat Lid on

Preheat Lid on Pause off

Pause off

- - - - - - - - - - -

- - - - - - - - - - In denat

In denat Hot Start off

Hot Start off

- - - Stage 1 - - - -

- - - Stage 1 - - - Number cycles 30

Number cycles 30 Step 94.0 º

Step 94.0 º Step 68.0 º

Step 68.0 º

- - - - - - - - - - -

- - - - - - - - - - Fina Extn

Post run Final hold 10.0

Post hold 10.0

Other templates available include Ice bucket (incubation at 4ºC), Ligation (incubation at 15ºC) and an RT-PCR template.

* * New progr a

-*New*-

2 STEP [2]

2 STEP TEMPLATE 501

2 STEP

3 STEP

3 STE

3 STEP 3 [ , to select ]

[ , to select ]

2 STEP TEMPLATE [501]

Run Program

Run Method

View Program

View Method [ , to select ]

[ , to select ]

Run program

Run Method View program

View Method

Copy program

Copy Method

[ , to select ]

[ , to select ][ , to select ]

2 STEP

4.0 º C 5m00

0m30 max

0m30 max 1m00 max

1m00 max

2.0 º C 5m00

2 STEP

ºC

- - -

STEP TEMPLATE

STEP

eated Lid 105 º C

eated Lid 105 º C reheat Lid on

reheat Lid on ause off

ause off

- - -

enat

ot Start off

tage 1

umber cycles 30

umber cycles 30 tep 94.0

tep 94.0 tep 55.0

tep 55.0 tep 72.0

tep 72.0

inal Extn72.0º C 5m00 inal hold 10.0 º C

ost hold 10.0 º C

Use the “Down Arrow” key to select an existing ‘Template’ program from the menu, e.g. ‘2 Step Template’ or ‘3 Step Template’.

Press the “Enter” key to accept it.

The Template is already defined and can be run as it is or easily copied, modified and saved under a new name.

Use the “Dow n Arrow” key to select ‘Copy Program’

Press the “Enter” key to accept it.

4.0º C 5m00

- - - -

m30 max

m30 max m30 max

º

m30 max m30 max

m30 max

- - -

2.0º C 5m00

15

Heated Lid 105ºC

Heated Lid 105ºC Preheat Lid on

Preheat Lid on

Preheat Lid on [ , to edit ]

[ , to edit ]

To give a program a name

Heated Lid 105ºC

Heated Lid 105ºC Preheat Lid on

Preheat Lid on

Preheat Lid on [ , to edit ]

[ , to edit ]

Heated Lid 105ºC

Heated Lid 105ºC Preheat Lid on

Preheat Lid on

Preheat Lid on [ , to edit ]

[ , to edit ]

[ , to edit ] [ to save ]

[ to save ]

To save the program

Heated Lid 105ºC

Heated Lid 105ºC Preheat Lid on

Preheat Lid on [ SAVE ? ]

[ SAVE ? ] [ YES or No ]

[ YES or No ]

Run Program

View Program

View Program [ , to select ]

[ , to select ]

UNNAMED #

DONNA

DONNA [7]

A new name can now be given to the copied program (see below).

To type in a name for the program, press "Enter" and a cursor will appear in the position shown.

Using the keypad, type in the name you wish to give the program.

The letters will scroll round with successive presses; for example the “2” key will give ‘A’ with one press; ‘B’ with two presses; ‘C’ with three presses and ‘2’ with fou r presses. Five presses will again give ‘A’.

When the name is complete, press "Enter"

There are now two options:

1. Save the program and run it as it is or

2. Edit the program then save and run it.

A program can only be run once it has been saved. Press the “End” key.

The prompt line will flash between 'SAVE?' and 'YES or NO'.

Press “Enter” for ‘Yes’ to save the changes you have made.

If you press “End” for ‘No’ then all the changes you have made will be lost.

The program is now ready to run and will be allocated with a unique ‘Speed dial’ number which can be used to rapidly locate the program from the main startup screen.

“Up Arrow” or “Down Arrow” keys to scroll through the remaining list of options.

16

To edit a field or step in an existing program

Programs [0]

Methods [0]

Information

Information Configuration

Configuration [ , to select ]

[ , to select ]

DAVID 8 [D]

DONNA 7

[ , to select ]

Run program

Run method View program

View method Edit program

Edit method Copy method

Copy program Delete program

Delete method Set password

Set password

Set password [ , to select ]

[ , to select ]

Edit a selected field or step

Heated Lid 105ºC

Heated Lid 105ºC Preheat Lid on

Preheat Lid on

Preheat Lid on [ , to edit ]

[ , to edit ]

[ , to edit ] [ to save ]

[ to save ]

DONNA [7]

DONNA

When the unit is switched on the display will show this screen.

If known, type in the ‘Speed dial’ number for the required program and press “Enter” This will take you directly to the required program.

If you do not know the ‘Speed dial’ number, press “Enter” to access the list of stored programs.

Either:

1. Scroll to the required program using the “Up Arrow” or “Down Arrow” keys

or

2. Type the first letter of the program name in the ‘New program’ field; this will then list all the program n ames beginning with that letter. Use the “Up Arrow” or “Down Arrow” keys to select the required program.

Press “Enter” to accept

The full set of options for a program is shown here; these are shown on the LCD screen three lines at a time.

Use the “Down Arrow” and/or the “Up Arrow” keys to select the option you require.

Press the “Enter” key to select the option.

Select ‘Edit program’ and a screen similar to this will appear.

Use the “Down arrow” and/or the “Up arrow” keys to select the field or step you want to edit.

You can now edit the active field or step by first pressing the “Enter ” key

17

º

C

º

C

º

C

º

C

Heated Lid 105ºC

Preheat Lid on

Preheat Lid on [ edit temperature ]

[ edit temperature ] [ to disable ]

[ to disable ]

Heated Lid 105ºC

Preheat Lid on

[ to change ]

[ to change ] [ turn on/off ]

[ turn on/off ]

Pause off

---------------------

In denat 94.0ºC 00m30

[ edit temperature ]

[ edit temperature ] [ to disable ]

[ to disable ]

Pause off

---------------------

In denat 94.0ºC 00m30

[ edit time ]

[ edit time ] [ . for hrs/mins ]

[ . for hrs/mins ]

---------------------

Post run

Fin extn

Fin hold 10.0

Post hold 10.0

[ , to edit ]

[ , to edit ] [ to save ]

[ to save ]

DONNA

72.0 º C 5m00

To edit a temperature, type in the required temperature. In the example here, the heated lid temperature is being edited.

Press “Enter” to accept.

Use the “Down Arrow” and/or the “Up Arrow” keys to select the next field or step you wish to edit.

To edit a field with on/off settings, first select the field and press “Enter”

Use the “Up Arrow” and “Down Arrow”

keys to toggle between the settings.

Press “Enter” to accept.

To edit a step with both a temperature and a time, first press “Enter” and type in the new temperature. Press “Enter” again. Now type in the new time. The default is minutes.

1. For whole minutes, type the number required then “Enter”

2. To toggle between minutes and hours, use the “Decimal Point” key.

3. For values less than 1 minute, first press the “Decimal Point” key followed by the number of seconds required.

4. For minutes and seconds, type the number of minutes followed by the “Decimal Po int” key then the number of seconds.

Once the values have been added, press “Enter” to accept.

Once you have finished editing and you are happy with the changes, press the “End” key followed by the “Enter” key to save.

If you press the “End” key a second time for ‘No’ then all the changes you have made will be lost.

18

To copy a selected program

UNNAMED #

Heated lid 105ºC

Heated lid 105ºC Preheat lid on

Preheat lid on

Preheat lid on [ , to edit ]

[ , to edit ]

[ , to edit ] [ to save ]

[ to save ]

To delete a selected program

delete program

[ SURE ? ]

[ SURE ? ] [ Yes or No ]

[ Yes or No ]

To set a password for a selected program

New passwd

New passwd [ to accept ]

[ to accept ] [ to exit ]

[ to exit ]

DONNA

If you select ‘Copy program’ a screen similar to this will appear and the software will automatically give the program the next number until it is named.

Press “Enter” and type in a new name. You will then be able to edit the program as previously described.

If you select ‘Delete program’ a screen similar to this will appear.

Press “Enter” to delete the program or “End” to return to the program screen.

If you want to password protect the program so that other users cannot edit it, select ‘Set password’. A screen similar to this will appear.

Type in the password you wish to give it and press “Enter” . The program list will then display the key symbol next to the name to show that it is locked.

Other users will still be able to copy/view your program but not edit it.

Option 2: Creating a new program

A second option is to create a completely new program without using templates.

Heated lid 105

Heated lid 105 Preheat lid off

Preheat lid off Pause off

Pause off

-

- - - - - - - - - -

In denat

In denat Hot Start off

Hot Start off

-

- - - - - - - - - -

-

- - - - - - - - - -

Fin extn

Post run Fin hold 10.0

Post hold 10.0

UNNAMED #

94.0

72.0 º C 5m00

C 5m00

º

ºC

A number of typic al values are pre-ente r ed including:

Heated lid temperature Preheat lid Initial denaturation Hot start Final extension Final hold These values can be edited and the

additional stages and steps required for cycling can be added by following the instructions detailed on the following pages.

19

Example programs

A couple of example thermal cycling programs are shown below.

Typical 3-Step PCR

Heated Lid 105 Preheat Lid on Pause off

- - - - - - - - - - - - - - - - In denat 94°c 5min Hot Start off

- - - - - - Stage 1 - - - - - - Number Cycles 30 Step 94°c 0m30 max Step 55°c 0m30 max step 72°c 0m30 max

- - - - - - - - - - - - - - - - Post run 72°c 5m Post hold 4ºC 10m

3-Step PCR with multiple ‘Stages’

Heated Lid 105 Preheat Lid on Pause off

- - - - - - - - - - - - - - - - In denat 94

Hot Start off

- - - - - - Stage 1 - - - - - - Number Cycles 10

step 94 step 60 step 72

- - - - - - Stage 2 - - - - - - Number Cycles 20

step 94 step 55 step 72

- - - - - - - - - - - - - - - - Post run 72

Post hold 4ºC 10m

°c 5min

°c 0m30 max °c 0m30 max °c 0m30 max

°c 5m

Programming

* * New program ** []

-*New*- []

2 STEP TEMPLATE 501

2 STEP 2 3 STEP TEMPLATE 502

3 STEP 3 [ , to select ]

[ , to select ]

Heated lid 105ºC

Heated lid 105ºC Preheat lid on

Preheat lid on

Preheat lid on [ , to edit ]

[ , to edit ]

[ , to edit ] [ to save ]

[ to save ]

To add a stage and then edit it

In denat 94.0ºC 5m00

In denat 94.0ºC 5m00 Hot Start off

Hot Start off

-----------

[ to insert stage ]

UNNAMED #

Create a new program by selecting

-* New *- from the menu and press “Enter”

Give the program a name and edit any of the default fields as described above.

Use the “Down Arrow” key to scroll to the dotted line below ‘Hot Start’; it will start to flash.

Press "Enter" to insert a stage

A new stage will be in se rt ed w h i ch consists of a ‘Number cycles’ field and one step.

20

-- Stage 1 ----------

-- Stage 1 ---------Number cycles

Number cycles Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max [ edit no. cycles ]

[ edit no. cycles ]

-- Stage 1 ----------

-- Stage 1 ---------Number cycles 10

Number cycles 10

Step 94.0ºC 00m30 max

[‘.’ to insert step ]

[‘.’ to insert step ] [ , to edit ]

[ , to edit ]

[ , to edit ] [ to delete step ]

[ to delete step ]

To insert additional steps

-- Stage 1 ----------

Number cycles 10

Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max [‘.’ to insert step ]

[‘.’ to insert step ]

[‘.’ to insert step ] [ , to edit ]

[ , to edit ]

To delete a step

-- Stage 1 ----------

-- Stage 1 ---------Number cycles 10

Number cycles 10

Step 94.0ºC 00m30 max

[‘.’ to insert step ]

[‘.’ to insert step ] [ , to edit ]

[ , to edit ]

[ , to edit ] [ to delete step ]

[ to delete step ]

-- Stage 1 ----------

-- Stage 1 ---------Number cycles 10

Number cycles 10

Step 94.0ºC 00m30 max

[ DEL STEP ? ]

[ DEL STEP ? ] [ Yes or No ]

[ Yes or No ]

Edit the number of cycles by typing the number required then press "Enter"

Use the “Down Arrow” key to move to the step.

Press “Enter” to edit the step as described above.

Press “Enter” again to accept.

To insert a new step before the first step, use the “Up Arrow” or “Down Arrow” keys to select ‘Number cycles’.

Press the “Decimal Point” key to insert a new step.

To insert a new step after an existing step, use the “Up Arrow” or “Down Arrow” keys to select the step.

Press the “Decimal Point” key to insert a new step.

Use the “Up Arrow” or “Down Arrow” keys to select the step you wish to delete.

Press "Delete" to delete the step.

The prompt line will flash between 'DEL STEP?' and 'YES or NO'.

Press “Enter” to delete the step. or Press “End” to return to editing the

step.

21

To add a new stage before an existing

-

º

C

-

º

C

-

º

C

one

-- Stage 1 ----------

Number cycles 10

Number cycles 10 Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max [ to insert stage ]

[ to insert stage ] [ to delete stage ]

[ to delete stage ]

To add a new stage at the end

- - - - - - - - - -

Post run 72.0 º C 5m00

Fin extn 72.0 º C 5m00 Post hold 10.0

Fin hold 10.0 [ to insert stage ]

[ to insert stage ]

To delete a stage

-- Stage 1 ----------

Number cycles 10

Number cycles 10 Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max [ to insert stage ]

[ to insert stage ] [ to delete stage ]

[ to delete stage ]

-- Stage 1 ----------

Number cycles 10

Number cycles 10 Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max [ DEL STAGE ? ]

[ DEL STAGE ? ] [ YES or No ]

[ YES or No ]

72.0 º C 5m00

Use the “Up Arrow” or “Down Arrow” keys to highlight the stage number.

Press "Enter"

A new stage will be added before the existing stage. The other stage numbers will be adjusted as appropriate. The new stage can now be edited as described above.

Use the “Up Arrow” or “Down Arrow” keys to highlight the dotted line below the last stage.

Press "Enter"

A new stage will be added with the next consecutive number. The new stage can now be edited as described above.

Use the “Up Arrow” or “Down Arrow” keys to highli ght the stage you wish to delete.

Press "Delete"

The prompt line will flash between 'DEL STAGE?' and 'YES’ or ‘NO'.

Press “Enter” to delete the stage.

22

To insert a ‘pause’ into a program

Step 94.0ºC 00m30 max

-- Stage 2 ----------

Number cycles

Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max [‘.’ to insert step ]

[‘.’ to insert step ] [ , to edit ]

[ , to edit ]

Step 94.0ºC 00m30 max

-- Stage 2 ----------

Number cycles 0

Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max [ CHANGE TO PAUSE ? ]

[ CHANGE TO PAUSE ? ] [ Yes or No]

[ Yes or No]

-- Stage 2 ----------

Pause

---------------------

--------------------[ to insert step ]

[ to insert step ] [ to delete step ]

[ to delete step ]

To insert a pause after an existing stage, follow the steps for ins erting a stage a s above.

Highlight the ‘Number cycles’ line then press “Pause”

The prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’.

Press “Enter” to insert a pause.

The stage number will then be highlighted with ‘Pause’ as the only line.

23

To program increment or decrement temperature or time.

Under normal circumstances, the Hold Temperature of all steps is constant. However, it is possible to automatically increment or decrement the temperature of a specified step of a programme.

Temperature

1

2

Cycle 1 Cycle 3Cycle 2 Cycle 4 Cycle 5

1

θ

3

2

1

3

θ+∂θ

2

1

3

θ+2∂θ

2

1

3

θ+3∂θ

2

1

3

θ+4∂θ

Temperature profile of incremented Hold Temperature at step 3 of each cycle

The Hold Temperature of the incremented/decremented step is the defined Hold Temperature plus the summation of the increments/decrements. The first cycle is never incremented/decremented, only subsequent cycles. If you select a decrement, the Hold Temperature is prevented from falling below 4°C. If you select an increment, the Hold Temperature is prevented from rising above 99°C.

Similarly, under normal circumstances, the Hold Time of all steps is constant. However, it is possible to automatically increment or decrement the duration of a specified step of a program.

1

3

Temperature

2

Cycle 1 Cycle 3Cycle 2 Cycle 4 Cycle 5

t

2

t+∂t

2

t+2∂t t+3∂t t+4∂t

2

Temperature profile of incremented Hold Time at step 3 of each cycle

The Hold Time of the incremented/decremented step is the defined Hold Time plus the summation of the increments/decrements. The first cycle is never incremented/decremented, only subsequent cycles. If you select a decrement, the Hold Time is prevented from falling below one second.

Number cycles 30

Number cycles 30 Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max

Step 58.0ºC 00m30 max

[ , to edit ]

[ , to edit ] [ to delete step ]

[ to delete step ] [‘.’ to insert step ]

[‘.’ to insert step ]

Use the “Up Arrow” or “Down” Arrow” keys to move to the step where you want an increment or decrement temperature or time.

24

Number cycles 30

Number cycles 30 Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max

Step 58.0ºC 00m30 max

[ CHANGE STEP ? ]

[ CHANGE STEP ? ] [ YES OR NO ]

[ YES OR NO ]

Number cycles 30

Number cycles 30 Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max

fst 55.0ºC 00m30 max

[ , to edit ]

[ , to edit ] [ to save ]

[ to save ]

Number cycles 30

Number cycles 30 Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max

fst 65. ºC 00m30 max

[ edit temperature ]

Step 94.0ºC 00m30 max

fst 65.0ºC 00m30 max

lst 55.0ºC 00m30

[ , to edit ]

[ , to edit ] [ to delete step ]

[ to delete step ] [‘.’ to insert step ]

[‘.’ to insert step ]

Step 94.0ºC 00m30 max

fst 65.0ºC 00m30 max

lst 55.0ºC 1m30

[ edit time ]

[ edit time ] [ . For hrs/mins ]

[ . For hrs/mins ]

Press “Enter” to go into edit mode.

Press “Pause”

The prompt line will change to flash between ‘CHANGE STEP?’ and ‘YES’ or ‘NO’.

Press “Enter”

The cursor will again move into the edit mode.

Now press the “Up Arrow” key

The step will change to ‘fst’, meaning “first”, and the software will in sert another line after it entitled ‘lst’, meaning “last”.

Press “Enter” twice to go into edit mode.

Use the “Alpha/numeric” keys to change the values of the ‘fst’ line to the first temperatures and/or times you require.

Press “Enter” after each field you change.

You will exit ‘Edit Mode’ when you reach the end of the line.

Use the “Down A rrow” key to move onto the ‘lst’ line.

Press “Enter” to edit the step.

Use the “Alpha/numeric” keys to change the values of the ‘lst’ line to the final temperatures and/or times you require.

Press “Enter” for each field you change.

You will exit ‘Edit Mode’ when you reach the end of the line.

When you run the program it will

25

To save the program

d

-- Stage 1 ----------

Number cycles 10

Number cycles 10 Step 94.0ºC 00m30 max

Step 94.0ºC 00m30 max [ SAVE ? ]

[ SAVE ? ] [ Yes or No ]

[ Yes or No ]

Run metho

Run Program

View Program

View method [ , to select ]

[ , to select ]

Stopping what you are doing

GEORGE [10]

automatica lly increment or decrement in equal steps from the first time and/or temperature to the last over the number of cycles in the stage. For example: if the first temperature is 65°C and the last temperature is 55°C, over 30 cycles the temperature will decrease 0.33°C each cycle. Similarly, in the example shown, the time would increase from 30 seconds to 1 minute 30 seconds in 2 second increments.

A program can only be run once it has been saved. Press the “End” key.

The prompt line will flash between 'SAVE?' and 'YES or NO'.

Press “Enter” for ‘Yes’ to save the changes you have made.

If you press “End” for ‘No’ then all the changes you have made will be lost.

The program is now ready to run and will be allocated with a unique “Speed dial” number which can be used to rapidly locate the program from the main start-up screen.

Use the “Up Arrow” and “Down Arrow” keys to scroll through the list of options.

At any time during programming the TCY , when you are satisfied with the program, you can finish and accept what you have done by first pressing the “End” key.

Then follow the instructions on screen. If you are not sure, wait for the instructions in the prompt line to scroll back to the first line that you see.

26

To run a program

d

Programs [0]

Methods [0]

Information

Information Configuration

Configuration [ , to select ]

[ , to select ]

Programs [7]

Methods [7]

Information

Information Configuration

Configuration [ , to select ]

[ , to select ]

* * New Methods ** []

* * New program ** []

2 STEP TEMPLATE 501

2 STEP 2 3 STEP TEMPLATE 502

3 STEP 3 [ , to select ]

[ , to select ]

DAVID 8 [D]

DONNA 7

[ , to select ]

Run metho

Run Program

View Program

View method [ , to select ]

[ , to select ]

[ RUN PROGRAM ? ]

[ RUN METHOD ? ] [ Yes or No ]

[ Yes or No ]

DONNA [7]

DONNA

When the unit is switched on the display will show this screen.

If known, type in the ‘Speed dial’ number for the required program and press “Enter” This will take you directly to the required program.

If you do not know the ‘Speed dial’ number, press “Enter” to access the list of stored programs.

Next either :

1. Scroll down to the required program using the “Up Arrow” and “Down Arrow” keys.

2. Or type the first letter of the program name in the “New program” field; this will then list all the program n ames beginning with that letter. Use the “Up Arrow” and “Down Arrow” keys to select the required program.

Press “Enter” to accept For example ‘Donna’.

To run the program, select ‘Run program’ and press "Enter"

A screen similar to this one will appear.

Press “Enter” to start the run.

27

Cycle 4/30 56m08

Cycle 4/30 56m08 Sample 58.0

Sample 58.0 Hold @ 58.0 º C 00m17

Hold @ 58.0

Hold @ 58.0 [ to STOP ]

[ to STOP ]

[ to STOP ] [ to PAUSE ]

[ to PAUSE ]

[ to PAUSE ] [ for info ]

[ for info ]

Manual pause

Cycle 24/30 19m26

Cycle 24/30 19m26 Sample 72.0

Sample 72.0 Hold @ 72.0

Hold @ 72.0

Hold @ 72.0 [ PAUSE ? ]

[ PAUSE ? ]

[ PAUSE ? ] [ YES or NO ]

[ YES or NO ]

Cycle 24/30 19m26

Cycle 24/30 19m26 Sample 72.0

Sample 72.0 Paused

Paused [ to STOP ]

[ to STOP ]

[ to STOP ] [ to CONTINUE ]

[ to CONTINUE ]

[ to CONTINUE ] [ for info ]

[ for info ]

Manual Stop

Cycle 28/30 10m39

Cycle 28/30 10m39 Sample 94.0

Sample 94.0

5.7 /S Hold @ 94.0

Hold @ 94.0

Hold @ 94.0 [ STOP PROGRAM

[ STOP PROGRAM

[ STOP PROGRAM [ YES or NO ]

[ YES or NO ]

º C

º

C 00m17

º

C

º

C 00m10

º

º C

C

º

C 00m10

º

? ]

During the run the LCD screen will report:

1. The cycle number

2. The time remaining (counting down in seconds)

3. The sample temperature (whether holding or ramping)

4. Maximal ramp rate

5. The current step of the run

6. A count down of the hold time.

An example is shown here.

To pause a program while it is running, press “Pause”

The run will continue but the prompt line will alternate ‘PAUSE?’ and ‘YES or NO’

To confirm that you want the program to pause press “Enter”

If you have pressed the “Pause” key by mistake and you want the program to continue, press “End”

The unit will emit a beeping sound while paused.

To resume the run, press “Pause” followed by “Enter” to continue.

To stop a program while it is running, press “End”

º

The run will continue but the prompt line will alternate ‘STOP PROGRAM?’ and ‘YES or NO’

To confirm that you want to stop the program press “Enter”

If you have pressed the “End” key by mistake and you want the program to continue, press “End” again.

When a program is stopped the unit will emit a beeping sound and the original program screen will appear.

28

Program Information

-

2

-

0

2

m

.

Heated Lid on

Heated Lid on Status ramping

Status ramping [ to exit ]

[ to exit ]

DONNA

8. Information

Programs [0]

Information

Configuration

Configuration [ , to select ]

[ , to select ]

Block 20x0.5ml

Block Type: 96x0. Block s/n

Block id Unit s/n

Unit s/n C4C123456 S/w version 14.zz

S/w version 6 H/w version 01

H/w version 2.ZZ Comms

Comms Unit cycles 624

Unit cycles 624 Block cycles 69

Block cycles 69

address 1

139160

123456789 GP0000

zz

Pressing “Enter” while a program is running will give information about the current run including which program is actually running and the unit status e.g. holding or ramping.

An example is shown here.

The Information field on the front screen contains all the unit-specific information such as serial number and software version.

Press “Enter” to access the unit information.

l

1

The complete list is displayed here.

Note that the “Comms address” must be unique for each unit if more than one TCY is linked to a PC

29

9. Configuration

Warning! The Configuration software should only be accessed by designated personnel, as some

parameters can be changed or may be lost when using it.

Programs [0]

Methods [0] Information

Information

Configuration

[ , to select ]

Password pad

Password pad [ to accept ]

[ to accept ] [ to exit ]

[ to exit ]

Program defaults

Settings Settings

Passwords Passwords

Format memory Controller reset

Self-calibrate

Settings

Program defaults

Settings

Passwords

Settings

Format memory

Passwords Controller reset

Self-calibrate

Program defaults

Settings

CONFIGURATION

Passwords

Format memory

Controller reset

Self-Calibrate

The Configuration field on the front screen allows a system administrator access to set up all the default settings for the programs, time and date settings and to change and remove passwords.

Access to the ‘Configuration’ set up is password protected.

Default admin passwords is 1234 To enter configuration, use the alpha keys

to type in ‘pad’

Press “Enter”

There are a number of options which can be changed; these are listed here.

Use the “Up Arrow” and “Down Arrow” keys to select then press “Enter” to access any of the options. Each option is discussed in detail below.

Highlight ‘Settings’ then press “Enter”

30

d

1920

Set date xx/xx/xx

Set time

Set time xx:xx

Auto restart on

Date format

Date format Auto restart on

Auto restart on

Beeper on

Coms address 1

address 1

Coms

Coms speed

Coms

Coms [ , to select ]

[ , to select ]

Passwords

Settings

Program defaults Settings

Passwords

Format memory

Controller reset

Self-calibrate

** Admin passwd **

DONNA

GEORGE

GEORGE [ , to select ]

[ , to select ]

Password

Password Confirm

Confirm

Confirm [ to accept ]

[ to accept ]

** Admin passwd **

** Admin passwd ** DONNA

DONNA

GEORGE

[ DEL PASSWORD ? ]

[ DEL PASSWORD ? ] [ YES NO ]

[ YES NO ]

address 1 speed 4800

speed 4800

ADMIN PASSWORD

xx:xx

my

The complete list of settings is given here.

Auto restart “on” will allow the unit to restart from where it left off should there be a power failure and the power returns.

The “Comms address” refers to the

0

location of a unit in a daisy chain and must be unique if there are several units linked to each other.

The optimum “Coms speed” is set at

19200. When using control software, ensure all TCY’s are set to the same value.

Edit any line as described for ‘Program defaults’ above. Press “Enter” to accept the new value.

When you have finished, press the “Enter” key to go back to the ‘Configuration’ menu.

The ‘Passwords’ function allows the administrator to change the ‘Admin password’ (which allows access to Configuration) and also to delete any passwords associated with programs.

Highlight ‘Passwords’ then press “Enter”

The screen will appear as opposite.

We suggest that you change the ‘Admin password’ at this stage in order to protect the configuration settings.

Use the “Up Arrow” key to highlight ‘Admin passwd’

Press “Enter” and type in the new password. Repeat to confirm and accept.

The screen will go back to the ‘Configuration’ menu.

To delete a password associated with a program, use the “Up Arrow” and “Down Arrow” keys to highlight the program.

Press “Enter”

Press “Enter” again to delete the password.

31

Controller reset

Settings

Program defaults Settings

Passwords Passwords

Format memory

Self-calibrate

Controller reset

Format Memory

CONTROLLER RESET

This will delete

This will delete all programs

all methods [ SURE ? ]

[ SURE ? ] [ YES NO ]

[ YES NO ]

The ‘Format memory’ will delete all saved programs from the unit.

Highlight ‘Format memory’ then press “Enter”

To delete all the programs, press “Enter” Set ADMIN PASSWORD to 1234.

10. Notes on transferring protocols from other thermal TCYs

The TCY heats at over 3.6°C/s and provides the highest levels of temperature uniformity. Many slower machines have historically begun the countdown on hold times before the set temperature is reached in order to avoid any overshooting in sample temperature. The slower the heating rate the more significant the loss in hold time, i.e. if a 30 second hold time is set at 55°C then perhaps 5 to 10s will be lost in between the start of the time countdown and actually reaching the set temperature. The overall effect of this method of control is that your sample, if cooling from a higher temperature, is subjected to an environment with an elevated temperature compared to the target temperature for a significant part of the hold time.

The TCY provides the user with ‘what-you-program-is what-you-get’. Due to the rapid and responsive nature of the Peltier heating system, the time between the timer countdown initiating and actually reaching the set temperature has been reduced to as little as 1s. This ensures that the sample is only subjected to the exact temperature required.

Taking the above into consideration it must therefore be noted that care must be taken when reoptimizing protocols from a slower machine.

Trouble Shooting

1. The optimum annealing temperature may in fact be higher than the set temperature used in the slower machine. Try increasing it by 1 or 2°C.

2. The hold time can be reduced once the optimum temperature has been found. Try reducing it by 5 to 10s.

32

11. User maintenance

Cleaning your TCY

The heating/cooling block, including wells and flat surfaces, should be cleaned regularly to ensure optimum heat transfer to the samples. Always clean the block if there has been a spillage. Use a cloth or cotton buds dipped in a fresh, 50:50 water/isopropanol solution and make sure that no deposits are left in the wells.

Heater Block removal

Before cleaning your unit, disconnect from the power supply and allow it to cool to below 50°C.

 Slide the quick release handles in the

direction of the arrows indicated and lift the block assembly out of the unit.

 Never lift or carry the block by one quick

release handle, always use both handles or support the block from underneath.

 When re-fitting the block, or fitting a new one,

slide the quick release handles in the opposite direction to lock the assembly into the unit.

 Open or closed padlock symbols are printed

on the quick release handles for easy reference.

The outer case of the TCY can be cleaned with a cloth dipped in water or ethanol (methanol or formaldehyde can also be used). No part of the case or cover should be immersed in the solvents. Do not use aggressive solvents such as acetone, or abrasive cleaners.

33

12. Some frequently asked questions

Q1 What is the ‘Pause’ function at the start of the program used for? A1

Q2 Why do you have to press some keys twice to make the unit do what you want it to

A2

Q3 What is ‘Hot Start’? A3

Q4 What is the incremental function? (See pages 29-31). A4

Q5 The heat-up ramp-rate in the specification is 3.6°C/second yet I can only manually set

A5

Some users prefer to preheat the heated lid before placing the samples into the unit. The pause feature is used to stop the unit after the 4 minute heated lid preheat step. It will also sound an audible alarm indicating that the machine is ready for the sample tubes or plate to be added. Pressing the “Pause” key followed by the “Enter” key will commence the remaining program.

do.?

With many of the program features there is a fail-safe function which asks if you are sure you want to perform a particular action. You are required to press one key to select a function followed by a second to accept it.

A “hot start” is used for reducing non-specific product formation during the first cycle of amplification. The ‘Hot Start’ programming step is used to pause the instrument at a specific temperature, typically around 70ºC, after the initial template denaturation. The reason is to allow the manual addition of unmodified Taq DNA polymerase which may loose activity if added during the initial 5min denaturation. Heat-activated Taq or Hot Start enzymes do not require this step.

Incremental timing and temperature are used to increase or decrease either the time or temperature incrementally over the number of cycles in a stage.

Incrementation of extension time is used with ‘Long PCR’ which is when large template fragments are to be amplified (e.g. 27kb lambda DNA, 40kb genomic DNA).

Decremental temperature is used for protocols such as ‘Touchdown PCR’ where one starts with a high annealing temperature in the first cycle and gradually decreases the temperature over the number of cycles in the stage. This ensures that only the specific product is amplified.

it to 2.0°C/second. Why is this?

If you set the ramp-rate to max the block will heat at 3.6°C/second the unit sets its own control parameters. If you program a ramp-rate the control parameters reduce the ramp-rate. This is a feature of the software and not the actual ramprate.

34

13. Additional information

Brief fault finding notes and a list of replacement parts are given in this section.

Do Not Remove the outer cover or dismantle this unit! There are no user serviceable parts within.

Fault Finding

Should you encounter problems with your TCY contact Omega Flow Engineering for assistance. Please include details of the fault observed and remember to return the unit in its original packing. Omega Engineering accepts no responsibility for damage to units which are not properly packed for shipping: if in doubt, contact your supplier, giving the full serial number of the unit and software version number (shown when the unit is first switched on).

Fuses

If neither the power light nor display on the front panel is lit, one of the two fuses may have blown. Check that there is no external cause, such as a faulty plug or lead. Check both fuses and replace the faulty fuse with a new one of the correct value (fuse values are given on the label next to the power inlet). Note that fuses should only be replaced by a qualified electrician.

The holder for the two fuses is built into the power input socket. First remove the power cable and then gently prise the fuse drawer open with a flat-bladed screwdriver or similar tool.

Each fuse can be removed by using the screwdriver as a lever. Exchange the faulty fuse in the fuse holder for a working fuse of the correct value. Finally, replace

the fuse drawer in the fuse compartment and push the drawer shut. Fuses which blow repeatedly indicate a serious fault and you should return the unit to your

supplier for repair.

The heated lid over-temperature cut-out

The heated lid is fitted with an independent circuit to protect it from overheating. In the unlikely event of an over-temperature problem with the lid, the unit is fitted with a thermal fuse which will remove power to the heater plate should the maximum temperature be exceeded.

Insulation testing

This equipment is fitted with RFI suppression circuitry. Any check of the electrical insulation by means of high voltage dielectric testing (for example as in BS EN 61010-1) must be carried out using only a DC voltage.

This unit contains semiconductor components which may be damaged by electric field effects.

Interchangeable blocks

The block can be removed and replaced by another of the same sort or of a different sort. The software will register the change and set up the unit for the new block.

Before removing the block, disconnect from the power supply and allow it to cool to below 50℃.

35

 Slide the quick release handles in the direction of

the arrows indicated and lift the block assembly out of the unit.

 Never lift or carry the block by one quick release

handle, always use both handles or support the block from underneath.

 When re-fitting the block, or fitting a new one, slide

the quick release handles in the opposite direction to lock the assembly into the unit.

 Open or closed padlock symbols are printed on the

quick release handles for easy reference.

If you have fitted a flat block for slide applications, you must set the heated lid to OFF.

36

WARRANTY/DISCLAIMER

OMEGA ENGINEERING, INC. warrants this unit to be free of defects in materials and workmanship for a period of 36 months from date of purchase. OMEGA’s WARRANTY adds an additional one (1) month grace period to normal three (3) year product warranty to cover handling and shipping time. This ensures that OMEGA’s customers receive maximum coverage on each product.

If the unit malfunctions, it must be returned to the factory for evaluation. OMEGA’s Customer Service Department will issue an Authorized Return (AR) number immediately upon phone or written request. Upon examination by OMEGA, if the unit is found to be defective, it will be repaired or replaced at no charge. OMEGA’s WARRANTY does not apply to defects resulting from any action of the purchaser, including but not limited to mishandling, improper interfacing, operation outside of design limits, improper repair, or unauthorized modification. This WARRANTY is VOID if the unit shows evidence of having been tampered with or shows evidence of having been damaged as a result of excessive corrosion; or current, heat, moisture or vibration; improper specification; misapplication; misuse or other operating conditions outside of OMEGA’s control. Components in which wear is not warranted, include but are not limited to contact points, fuses, and triacs.

OMEGA is pleased to offer suggestions on the use of its various products. However, OMEGA neither assumes responsibility for any omissions or errors nor assumes liability for any damages that result from the use of its products in accordance with information provided by OMEGA, either verbal or written. OMEGA warrants only that the parts manufactured by the company will be as specified and free of defects. OMEGA MAKES NO OTHER WARRANTIES OR REPRESENTATIONS OF ANY KIND WHATSOEVER, EXPRESSED OR IMPLIED, EXCEPT THAT OF TITLE, AND ALL IMPLIED WARRANTIES INCLUDING ANY WARRANTY OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE HEREBY DISCLAIMED. LIMITATION OF LIABILITY: The remedies of purchaser set forth herein are exclusive, and the total liability of OMEGA with respect to

emnification, strict liability or otherwise, shall not exceed the purchase price of the

ind co mpone nt upon which liab ility is based. In no event s hall OMEGA be liable for consequential, incidental or special damages.

CONDITIONS: Equipment sold by OMEGA is not intended to be used, nor shall it be used: (1) as a “Basic Component” under 10 CFR 21 (NRC), used in or with any nuclear installation or activity; or (2) in medical applications or used on humans. Should any Product(s) be used in or with any nuclear installation or activity, medical application, used on humans, or misused in any way, OMEGA assumes no responsibility as set forth in our basic WARRANTY/DISCLAIMER language, and, additionally, purchaser will indemnify OMEGA and hold OMEGA harmless from any liability or damage whatsoever arising out of the use of the Product(s) in such a manner.

this order, whether based on contract, warranty, negligence,

RETURN REQUESTS/INQUIRIES

Direct all warranty and repair requests/inquiries to the OMEGA Customer Service Department. BEFORE RETURNING ANY PRODUCT(S) TO OMEGA, PURCHASER MUST OBTAIN AN AUTHORIZED RETURN (AR) NUMBER FROM OMEGA’S CUSTOMER SERVICE DEPARTMENT (IN ORDER TO AVOID PROCESSING DELAYS). The assigned AR number should then be marked on the outside of the return package and on any correspondence.

The purchaser is responsible for shipping charges, freight, insurance and proper packaging to prevent breakage in transit.

FOR WARRANTY following information available BEFORE contacting OMEGA:

1. Purchase Order number under which the product was PURCHASED,

2. Model and serial number of the product under warranty, and

3. Repair instructions and/or specific problems relative to the product.

OMEGA’s policy is to make running changes, not model changes, whenever an improvement is possible. This affords our customers the latest in technology and engineering.

reproduced, translated, or reduced to any electronic medium or machine-readable form, in whole or in part, without the prior written consent of OMEGA ENGINEERING, INC.

RETURNS, please have the

FOR NON-WARRANTY REPAIRS, for current repair charges. Have the following information available BEFORE contacting OMEGA:

1. Purchase Order number to cover the COST of the repair,

2. Model and serial number of the product, and

3. Repair instructions and/or specific problems relative to the product.

consult OMEGA

Where Do I Find Everything I Need for

Process Measurement and Control?

OMEGA…Of Course!

Shop online at omega.com

SM

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M-5379/0114