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User Manual
Digital Compound
Binocular LED Microscope
Model MD8211E30
MicroscopeNet.com
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Table of Contents
i. Caution...................................................................................................... 1
ii. Care and Maintenance .............................................................................. 2
1. Components Illustration ............................................................................ 3
2. Installation ................................................................................................. 4
3. Operation .................................................................................................. 6
4. Specifications ............................................................................................ 8
5. Troubleshooting Guide .............................................................................. 9
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i. Caution
1. Open the carton carefully with a knife or paper cutter. Find the “UP” sign and place
the Styrofoam container on the side that makes the arrow upward. If the “UP” sign
is missing, please open the Styrofoam container gently to prevent any accessory
items (i.e. objectives or eyepieces) from dropping and being damaged.
2. Do not discard the molded Styrofoam container. The container should be retained
should the microscope ever requires reshipment.
3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty
environments. Ensure that the microscope is located on a smooth, level and firm
surface.
4. If any specimen solutions or other liquids splash onto the stage, objective or any
other component, disconnect the power cord immediately and wipe up the spillage.
Otherwise, the instrument may be damaged.
5. All electrical connectors (power cord) should be inserted into an electrical surge
suppressor to prevent damage due to voltage fluctuations.
6. For safety when replacing the LED light and the fuse, be sure the main switch is
off, unplug the power cord, and replace the LED light and the fuse
7. Confirm that the input voltage indicated on your microscope corresponds to your
line voltage. The use of a different input voltage other than that as indicated will
cause severe damage to the microscope.
8. Note: please read the instruction of the operation of camera in manual 3.9 below
and the CD in the package before you start to use it.
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ii. Care and Maintenance
1. Do not attempt to disassemble any component including eyepieces, objectives or
focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on
metal surfaces should be cleaned with a damp cloth. More persistent dirt should be
removed using a mild soap solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using
an air stream from an air bulb. If dirt remains on the optical surface, use a soft cloth
or cotton swab dampened with a lens cleaning solution (available at camera stores).
All optical lenses should be swabbed using a circular motion. A small amount of
absorbent cotton wound on the end of a tapered stick makes a useful tool for
cleaning recessed optical surfaces. Avoid using an excessive amount of solvents as
this may cause problems with optical coatings or cemented optics or the flowing
solvent may pick up grease making cleaning more difficult.
4. Store the instrument in a cool, dry environment. Cover the microscope with the dust
cover when not in use.
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1. Components Illustration
1 Eyepiece 8 Light Collector 15 Fine Focus Knob
2 Diopter Ring 9 Microscope Base 16 X-Y Stage Moving Knobs
3 Eyepiece Tube 10 USB Cable 17 Power Switch
4
Viewing Head w Built-in Camera
11 Head Lock Thumb Screw 18 Brightness Intensity Dial
5 Objective 12 Nosepiece 19 Condenser Lock Thumb Screw
6 Slide Holder 13 Microscope Body 20 Condenser Focus Knob
7 Mechanical Stage 14 Coarse Focus Knob 21 Condenser
1
10
2
3
5
6
7
8
11
12
13
14
15
16
17
18
19
20
21
9
4
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2. Installation
2.1 Installation of the digital binocular viewing head
1) Loosen the head lock thumb screw on the top of the microscope body and remove
the plastic cover on the top.
2) Remove the cap on the dovetail of the digital binocular viewing head.
3) Seat the dovetail of the viewing head into the socket on the top of the microscope
body and tighten the head lock thumb screw.
Caution:
Do not release the viewing head from your hand grip until you are sure the viewing
head is installed securely.
2.2 Installation of the eyepieces
1) Remove the protective caps from the eyepiece tubes.
2) Insert the eyepieces into the eyepiece tubes.
2.3 Installation of the objectives
1) Adjust the coarse focus knob until the mechanical stage is at its lowest position.
2) Turn the caps counter-clockwise to remove them from the nosepiece.
3) Take the objectives out from the plastic cases and turn each one clock-wise into the
holes on the nosepiece. Install the 4X objective into the nosepiece first. Then in a
counter-clockwise direction, rotate the nosepiece and install each succeeding higher
magnification objective.
Note:
Inspect the objectives frequently for dirt or oil; clean if necessary.
Use the 10X objective to initially focus the image of your specimen.
When changing the objective magnification, rotate the objective nosepiece until you
hear a “click” sound or have a clear "in position" feeling. This ensures the objective
is centered in the optical light path.
2.4 Replacing the fuse
1) Turn off the power switch and disconnect the power cord.
2) Find the fuse holder at the rear bottom of the microscope body.
3) Pry out the fuse holder gently with a flat head screwdriver.
4) Replace the old fuse with a new one, and then insert it back in. See Fig. 1.
2.5 Installation of the glass filter
1) Swing out the color filter holder under the condenser.
2) Place the filter into the color filter holder as shown in Fig. 2, swing the holder in.
Fig.1
Fuse Holde
r
Fuses
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2.6 Replacing the LED lamp
1) Turn off the power switch and disconnect the power cord.
2) Loosen the 7 screws on the bottom board. See Fig 3 (a)
3) Open the board and find the LED lamp. See Fig 3 (b)
4) Loosen the 2 screws and unplug the LED connector.
5) Replace the dead LED lamp with a new one.
6) Tighten the 2 screws and plug in the LED connector.
7) Put the board back its place.
8) Tighten the 7 screws.
Fig.2
Color Filter
Color Filter Holder
Fig.3
7 Screws
2 Screws
LED Lamp
LED Connector
(a)
(b)
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3. Operation
3.1 Adjusting illumination
1) Plug the power cord into the power socket on the microscope and connect it to the
power outlet.
2) Turn on the power switch.
3) Rotate the brightness intensity dial to increase or decrease the brightness of the
illuminator.
Caution:
A diffusion filter is attached beneath the condenser to get uniform light and protect
your eyes from strong LED light when a low power objective applies. The diffusion
filter can be swung out to make the view field brighter when observing with a high
power objective, such as 100X objective.
3.2 Placing specimen
1) Place the slide on the mechanical stage.
2) Use the slide holder to gently secure the slide.
3) Turn the X and Y stage moving knobs to position the specimen in the center of
viewing field.
Caution:
Be sure not to allow an objective to touch a specimen slide when changing
objectives.
3.3 Focusing
1) With the 10X objective in position, raise the mechanical
stage using the coarse focus knob until the specimen is
close to the objective.
2) Turn the coarse focus knob until the specimen is in
focus. Then use the fine focus knob to obtain a sharp
image. You may now switch to another magnification
objective.
3) To get a good focused image, you may need to
combine the focus knob adjustment and interpupillary
distance adjustment, along with eyepiece diopter
adjustment stated in 3.4 and 3.5.
Tips:
T
o prevent your specimen slide from making contact with an objective, turn the 40X
objective in position and adjust the thumb screw of focus stop (as shown in Fig. 3)
so that the 40X objective will not contact the specimen while the stage is adjusted to
its highest position. Give the stage a tiny extra moving space to ensure the objective
can be focused every time.
3.4 Adjusting interpupillary distance
While observing with both eyes, hold the left and right eyepiece tubes then swing
them around the center axis. The interpupillary distance is correct when the left and
right fields of view converge completely into one image.
3.5 Adjusting eyepiece diopter
1) Using the 10x objective and your right eye only, observe your specimen through the
eyepiece and bring it into focus by adjusting the focus knobs.
Fig.3
Focus Stop
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2) Then observe the specimen with your left eye only through the left eyepiece. If the
specimen is not in focus, turn the diopter ring on the eyepiece tube until a sharp
image is obtained.
3.6 Adjusting condenser
1) Turn the condenser focus knob to raise or lower the condenser.
2) The condenser is raised when using high magnification objectives and lowered
when using low magnification objectives.
Note:
The centering of the condenser and the light axis of the objective are factory
adjusted. Do not attempt to re-adjust.
The highest position of the condenser has been factory adjusted. Do not attempt to
re-adjust.
3.7 Adjusting iris aperture diaphragm
Swing the iris diaphragm lever (Fig. 4) left or right to
adjust the aperture size.
Note:
The iris diaphragm is designed to adjust the aperture
size, not to adjust the brightness although the
brightness will be changed when it's adjusted. When
aperture is adjusted to smaller size, the contrast will be
increased and the depth of field will be increased as
well. Turn up the intensity of the light if the image is
too dim.
3.8 Adjusting focus knob tension
The tightness of the focus knob tension has
been pre-set at the factory. If the mechanical
stage drops by itself, rotate the tension
adjustment ring (Fig. 5) located inside the focus
knob on the power switch side until the tension is
in maintained.
3.9 Photo/video observing, capturing and
recording
1) Insert the USB cable into the USB port (Fig. 6) on the
back of the viewing head, and the other end to the
computer.
2) Turn on the computer, install the camera following the
manual in the mini CD.
3) Bring the microscope into focus by following the
procedures in 3.3.
4) Open image observing software to examine.
5) You also can capture images or record live videos
through the software, depending on the functions
provided by the software.
Note:
For the details of installation and operation of the camera and its software, please
refer to the manual in the mini CD.
Iris Diaphragm Lever
Fig.4
Fi
g
.5
Tension adjustment Ring
Fig.6
USB Port
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4. Specifications
Model MD8211E30
Total Magnification 40X, 100X, 200X, 400X
Viewing Head
30º inclined, 360º swiveling siedentopf binocular viewing head
with built-in 3.0MP camera
Interpupillary Distance
Hinge adjustment, 48mm - 75mm
Diopter Adjustment On left eyepiece tube
Eyepieces 1 pair of WF10X/18
Objective Tube Length 160mm
Nosepiece Reversed revolving quadruple
Objectives
Achromatic DIN 4X/0.10 160/0.17
10X/0.25 160/0.17
20X/0.40 160/0.17 (spring)
40X/0.65 160/0.17 (spring)
Condenser
Abbe, NA=1.25, w/ iris aperture diaphragm and filter holder
Rack and pinion focus adjustment
Focus Mechanism Coaxial coarse and fine focusing knobs on both sides w/ stop
Stage
Double layer mechanical stage
Dimension: 5-1/2” x 4-5/16” (140mm x 110mm)
Translation range: 3” x 1-5/8” (78mm x 40mm)
Illumination Transmitted: 3W LED, variable intensity
Camera
Built-in USB2.0 2048 x 1536 pixel (3.0MP)
Driver and Software included in the CD
Compatible w/ Windows 2000/XP/Vista/Windows7 (32/64-bit)
Power Supply AC 100-240V 50/60Hz (US and Canada plug)
Fuse 0.5A 250V
Dimension 12” x 8” x 15” (31cm x 20cm x 39cm)
Net weight 11 lb 14.5 oz (5.4 kg)
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5. Troubleshooting Guide
GENERAL PROBLEMS
Problem Cause Solution
Lamp does not
light when
switched on
No electrical power Check power cord connection
LED or power unit dead
Replace LED light
Fuse blown out Replace fuse
Darkness at the
periphery or
uneven
brightness in the
field of view
Revolving nosepiece not in click
stop position
Revolve the nosepiece to click-stop
position by swinging the objective
correctly into the optical path
Dirt or dust on
the view
Dirt or dust on the eyepiece,
condenser, objective, collector lens
or specimen
Clean the lens with a lens cleaning
paper
Poor image
quality or not
able to get
focused image
No slide cover attached to the slide Attach a 0.17mm slide cover
Slide cover is too thick or thin
Use a slide cover of the appropriate
thickness
(
0.17mm)
Slide may be upside down
(
specimen at the bottom
)
Turn slide over so the cover-glass faces
up
Diopter adjustment is not set
properly
Readjust the diopter settings
Condenser aperture is closed or
o
p
en too much
Open or close properly
Condenser is positioned too low Position the condenser upward
Specimen rises from stage surface Secure the specimen in the slide holder
Revolving nosepiece is not in the
click-sto
p p
osition
Revolve the nosepiece to the click-stop
p
osition
Lamp intensity is too high or low
Adjust the light intensity by rotating the
intensit
y
control dial
Slippage of focus
when using the
coarse focusing
knob Fine focus
is ineffective
Tension adjustment is set too low
Increase the tension on the focusing
knobs
Tension adjustment is set too high
Loosen the tension on the focusing
knobs
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