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User Manual
Binocular Compound
Model M828T
Microscope
MicroscopeNet.com
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Table of Contents
i. Caution……..……………………………………………………………3
ii. Care and Maintenance……….………………………………….….…4
1. Component Illustration………………………….…………………..…5
2. Installation.……………………………………………………………...6
3. Operation……………………….………………………….…………....8
4. Specifications…………………….…………………………………....10
5. Troubleshooting Guide…...............................................................11
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i. Caution
1. Find the “UP” sign and place the Styrofoam container on your table or bench so
that the arrow is pointing upward. Open the shipping carton carefully to prevent
any accessory items (i.e. objectives or eyepieces) from dropping and being
damaged.
2. Do not discard the molded Styrofoam container. The container should be retained
should the microscope ever requires reshipment.
3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty
environments. Ensure that the microscope is located on a smooth, level and firm
surface.
4. If any specimen solutions or other liquids splash onto the stage, objective or any
other component, disconnect the power cord immediately and wipe up the spillage.
Otherwise, the instrument may be damaged.
5. Important: the lamp, lamp housing and adjacent parts will become very hot. Do
not touch these parts until they have completely cooled. Never attempt to handle a
hot halogen bulb.
6. All electrical connectors (power cord) should be inserted into an electrical surge
suppressor to prevent damage due to voltage fluctuations.
7. For safety when replacing the halogen lamp or fuse, be sure the main switch is off,
unplug the power cord, and only replace the halogen bulb after the bulb and the
lamp house has completely cooled.
8. Confirm that the input voltage indicated on your microscope corresponds to your
line voltage. The use of a different input voltage other than that as indicated will
cause severe damage to the microscope.
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ii. Care and Maintenance
1. Do not attempt to disassemble any component including eyepieces, objectives or
focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on
metal surfaces should be cleaned with a damp cloth. More persistent dirt should be
removed using a mild soap solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using
an air stream from an air bulb. If dirt remains on the optical surface, use a soft cloth
or cotton swab dampened with a lens cleaning solution (available at camera
stores). All optical lenses should be swabbed using a circular motion. A small
amount of absorbent cotton wound on the end of a tapered stick makes a useful
tool for cleaning recessed optical surfaces. Avoid using an excessive amount of
solvents as this may cause problems with optical coatings or cemented optics or
the flowing solvent may pick up grease making cleaning more difficult. Oil
immersion objectives should be cleaned immediately after use by removing the oil
with lens tissue or a clean, soft cloth.
Store the instrument in a cool, dry environment. Cover the microscope with the
4.
dust cover when not in use.
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1 Components Illustration
1
2
3
4
5
6
7
8
9
19
20
1 Eyepiece 9 Microscope Base 17 Intensity Dial
2 Diopter Ring 10 Viewing Head 18 Power Switch
3 Eyepiece Tube 11 Head Thumb Screw 19 Condenser Focus Knob
4 Nosepiece 12 Microscope Body 20 Focus Stop Lever
5 Objective 13 Focus Tension Ring 21 Abbe Condenser
6 Slide Holder 14 Coarse Focus Knob
7 Mechanical Stage 15 Fine Focus Knob
8 Collector Lens 16 Stage Translational Knobs
10
11
12
13
14
15
16
17
18
21
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2 Installation
2.1 Installation of the binocular viewing head
1) Loosen the thumb screw on the head of the body and remove the plastic cover on
the top
2) Remove the cap on the dovetail of the binocular viewing head
3) Insert the binocular viewing head into the head of the body; ensure that the dovetail
is completely seated into the socket; tighten the thumb screw.
Caution: Do not release the head from your hand grip until you are sure the head is
installed securely.
2.2 Installation of the eyepieces
1) Remove the protective caps from the eyepiece tubes.
2) Insert the eyepieces into the eyepiece tubes.
2.3 Installation of the objectives
1) Adjust the coarse focus knob until the mechanical stage is at its lowest position.
2) Install the 4x objective into the nosepiece. Then in a clock-wise direction, rotate the
nosepiece and install each succeeding higher magnification objective.
Note: Inspect the objectives frequently for dirt or oil; clean if necessary. Use the 10x
objective to initially focus the image of your specimen. When changing the objective
magnification, rotate the objective nosepiece until you hear a “click” sound. This
ensures the objective is centered in the optical light path.
2.4 Installation of filter
1) Swing out the filter holder under the condenser.
2) Insert the filter into the holder, swing the holder in.
2.5 Installing (or changing) the halogen bulb
1) Turn the power off and disconnect the power cord.
2) Allow some time to cool down the lamp.
3) Turn over the microscope on its side; find the bulb compartment at
the bottom.
4) Open the cover of the bulb compartment by loosening the thumb
screw. Take out the dead bulb and insert the new bulb. Be sure
the pins on the bulb are completely inserted into the lamp socket.
You may also loosen the two screws on the cover to adjust the
position of the bulb to get centered and even brightness. Screw the cover on.
Caution: Before you turn over the microscope, be sure to take the eyepieces off and be
certain that the head is securely locked by the thumb screw.
2.6 Replacing the fuse
1) Turn off the power and disconnect the power cord.
2) Turn over the microscope on its side; find the fuse at the bottom of the base.
3) Turn the fuse holder counter-clockwise to take it off, insert new fuse, and then turn it
on clockwise.
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Caution: Before you turn over the microscope, be sure to take the eyepieces off and be
certain that the head is securely locked by the thumb screw.
2.7 Installing the mirror (optional, your model may not have one)
1) Unplug the power cord.
2) Screw off the light cover on the base.
3) Screw the black disc onto the base and then insert the mirror
into the hole at the center of the black disc. You may try to get
reflected ambient light on either side of the mirror with different
angles for best result.
Note: The mirror is only used when there is a power failure or you are in the field and no
power is available.
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3 Operation
3.1 Adjusting illumination
1) Connect the power cord and turn on the main switch.
2) Turn the variable intensity dial to increase or decrease the brightness.
3.2 Placing specimen
1) Place the slide on the mechanical stage.
2) Use the slide holder to gently secure the slide.
3) Turn the X and Y translational knobs to position the specimen for viewing.
Caution: Be sure not to allow an objective to touch a specimen slide when changing
objectives.
3.3 Adjusting interpupillary distance
While observing with both eyes, hold the left and right eyepiece tubes then slowly
slide the tubes in and out. The interpupillary distance is correct when the left and
right fields of view converge completely into one
image.
3.4 Adjusting eyepiece diopter
1) Rotate the 10x objective into position.
2) Rotate the diopter ring on the right eyepiece tube
until its numerical value is the same as your
interpupillary distance, for example, 65 in the
right figure.
3) Close your left eye and bring the specimen into
focus following the focusing procedures in 3.5.
4) Close your right eye and bring the same
specimen into clear sharp focus by adjusting the
diopter ring on left eyepiece tube only. Do not
use focus knobs at this step.
5) Since both sides are adjustable, you may also do
the above in the opposite way, in other words, left
eye first and right eye second.
65
3.5 Focusing
1) With the 10x objective in position, raise the mechanical stage using the coarse focus
knob until the specimen is close to the objective
2) Turn the coarse focus knob until the specimen is in focus.
3) Use the fine focus knob to obtain a sharp image.
4) You may now switch to another magnification objective.
Tips: To prevent your specimen slide from making contact with an objective, raise the
stage to its highest position without contacting the 100x objective, then tighten the
upper limit mechanical stage lever.
3.6 Adjusting condenser
1) Turn the condenser focus knob to raise or lower the condenser.
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2) Raise the condenser when using high power objectives and lower it when using low
power objectives.
Note:
The centering of the condenser and the light axis of the objective are factory
adjusted. Do not attempt re-adjustment.
The highest position of the condenser has been factory adjusted. Do not attempt
re-adjustment.
3.7 Adjusting aperture iris diaphragm
Move the iris diaphragm lever left or right to adjust the aperture size.
Note:
3.8 Adjusting focus tension
The iris diaphragm is designed to adjust the aperture size,
not to adjust brightness. Generally, opening the diaphragm to
70-80% of the N.A. value of the respective objective will
provide an image of acceptable quality. If you want to
observe the image of the iris diaphragm, remove one
eyepiece and look through the tube. You will see a dark circle
encroaching on the bottom of the tube.
The focus tension has been pre-set at the factory. If the mechanical stage drops by
itself, rotate the tension adjustment ring situated between the coarse focus knob and
microscope body on the power switch side until the tension is in maintained.
Iris Diaphragm
Lever
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4 Specifications
General
Model M828T
Total Magnification 40X, 64X, 100X, 160X, 400X, 640X, 1000X, 1600X
Viewing Head Binocular, inclined 45º, swiveling 360º
Interpupillary Distance Sliding adjustment, 55mm-75mm
Diopter Adjustment On both eyepiece tubes
Eyepieces 1 pair of WF10X/18
1 pair of P16X/11
Objective Tube Length 160mm
Nosepiece Revolving quadruple
Objectives Achromatic 4X, 10X, 40X(spring ), 100X(spring, oil)
Condenser Abbe, NA=1.25, w/ iris diaphragm and filter holder
Rack and pinion adjustment
Focus Mechanism Coaxial coarse and fine focusing knobs on both sides w/ focus stop
Minimum fine focusing adjustment at 0.002mm, range 28mm
Stage Mechanical stage
Dimension: 5-1/2” x 5-1/2” (140mmx140mm)
Movement range: 3” x 2” (75mm X 50mm)
Illumination Transmitted: 6V/20W, Halogen, Variable intensity
Power Supply AC 100V-240V, 50/60HZ (US and Canada plug)
Dimension 11” x 7-1/2” x 15-3/4” (28cm x 19cm x 40 cm)
Net weight 11 lb (5 kg)
Eyepieces
Designation Magnification Field of View Focal Length
Wide Field 10X 18mm 24.94mm
Plan Field 16X 11mm 15.58mm
Objectives
Magnification NA slide cover Thickness Focus Working Distance Working Mode
4X 0.10 0.17mm 31.04mm 37.5mm Dry
10X 0.25 0.17mm 17.13mm 7.316mm Dry
40X(S) 0.65 0.17mm 4.65mm 0.632mm Dry
100X(S, Oil) 1.25 0.17mm 2.906mm 0.198mm Oil
Magnifications
Eyepiece 10X 16X 10X 16X 10X 16X 10X 16X
Objective 4X 10X 40X 100X
Magnification 40X 64X 100X 160X 400X 640X 1000X 1600X
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5 Troubleshooting Guide
OPTICAL PROBLEMS
Problem Cause Solution
Darkness at the
periphery or uneven
brightness in the
field of view
Dirt or dust on the
view
Poor image quality
Revolving nosepiece not in click
stop position
The light source of the bulb is not at
the center
Dirt or dust on the lens eyepiece,
condenser, objective, collector lens
or specimen
No slide cover attached to the slide Attach a 0.17mm slide cover
Slide cover is too thick or thin
Slide may be upside down
Immersion oil is on a dry objective
(especially the 40x)
No immersion oil used with 100x
objective
Air bubbles in immersion oil Remove bubbles
Condenser aperture is closed or
open too much
Revolve the nosepiece to click-stop
position by swinging the objective
correctly into the optical path
Adjust the position of the bulb
Clean the lens with a camera
cleaning kit
Use a slide cover of the appropriate
thickness (0.17mm)
Turn slide over so the cover-glass
faces up
Check the objectives, clean if
necessary
Use immersion oil
Open or close properly
Problem Cause Solution
Lamp does not light
when switched on
Condenser is positioned too low Position the condenser upward
ELECTRICAL PROBLEMS
No electrical power Check power cord connection
Lamp bulb burnt out Replace bulb
Fuse blown out Replace fuse
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IMAGE PROBLEMS
Problem Cause Solution
Specimen rises from stage surface
Image moves while
focusing
Image tinged yellow
Image is too bright Lamp intensity is too high
Insufficient
brightness
Revolving nosepiece is not in the
click-stop position
Blue filter not used Use daylight blue filter
Lamp intensity is too low
Lamp intensity is too low
Aperture diaphragm closed too far Open to the proper setting
Condenser position too low Position the condenser upward
MECHANICAL PROBLEMS
Problem Cause Solution
Secure the specimen in the slide
holder
Revolve the nosepiece to the
click-stop position
Adjust the light intensity by rotating
the intensity control dial
Adjust the light intensity by rotating
the intensity control dial
Adjust the light intensity by rotating
the intensity control dial
Image will not focus
with high power
objectives
High power
objective contacts
slide when changed
from low power
objective
Slippage of focus
when using the
coarse focusing
knob Fine focus is
ineffective
Slide upside down
Cover glass is too thick Use a 0.17mm cover glass
Slide upside down
Cover glass is too thick Use a 0.17mm cover glass
Diopter adjustment is not set
properly
Tension adjustment is set too low
Tension adjustment is set too high
Turn the slide over so the cover
glass faces up
Turn the slide over so the cover
glass faces up
Readjust the diopter settings
Increase the tension on the
focusing knobs
Loosen the tension on the focusing
knobs
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