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User Manual
Binocular Compound
LED Microscope
Model M827TL Series
MicroscopeNet.com
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Table of Contents
i. Caution .................................................................................................... 1
ii. Care and Maintenance............................................................................. 2
2 Components Illustration ........................................................................... 3
3 Installation ............................................................................................... 4
4 Operation ................................................................................................. 6
5 Specifications ........................................................................................ 10
6 Optional Parts ........................................................................................ 11
7 Troubleshooting Guide .......................................................................... 13
8 Darkfield Condenser Installation and Operation Instructions .................. 15
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i. Caution
1. Open the carton carefully with a knife or paper cutter. Find the “UP” sign and place
the Styrofoam container on the side that makes the arrow upward. If the “UP” sign
is missing, please open the Styrofoam container gently to prevent any accessory,
i.e. objectives or eyepieces, from dropping and being damaged.
2. Do not discard the molded Styrofoam container. The container should be retained
should the microscope ever requires reshipment.
3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty
environments. Ensure that the microscope is located on a smooth, level and firm
surface.
4. If any specimen solutions or other liquids splash onto the stage, objective or any
other component, disconnect the power cord immediately and wipe up the spillage.
Otherwise, the instrument may be damaged.
5. All electrical connectors (power cord) should be inserted into an electrical surge
suppressor to prevent damage due to voltage fluctuations.
6. Confirm that the input voltage indicated on your microscope corresponds to your
line voltage. The use of a different input voltage other than that as indicated will
cause severe damage to the microscope.
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ii. Care and Maintenance
1. Do not attempt to disassemble any component including eyepieces, objectives or
focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on
metal surfaces should be cleaned with a damp cloth. More persistent dirt should be
removed using a mild soap solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using
an air stream from an air bulb. If dirt remains on the optical surface, use a soft cloth
or cotton swab dampened with a lens cleaning solution (available at camera
stores). All optical lenses should be swabbed using a circular motion. A small
amount of absorbent cotton wound on the end of a tapered stick makes a useful
tool for cleaning recessed optical surfaces. Avoid using an excessive amount of
solvents as this may cause problems with optical coatings or cemented optics or
the flowing solvent may pick up grease making cleaning more difficult. Oil
immersion objectives should be cleaned immediately after use by removing the oil
with lens tissue or a clean, soft cloth.
4. Observe the specimen with the 4X, 10X and 40X objectives in order, then observe
the specimen with the 100X objective. Apply the immersion oil on the slide cover
with the 100X objective. Do not let the immersion oil to contact with the dry
objectives lens (especially the 40X). Clean the dry objective lens using the camera
cleaning kit if the immersion oil is on the dry objectives lens. Clean the 100X
objective lens first using the camera cleaning kit after observing the specimen with
the 100X objective, then clean the specimen. More persistent dirt should be
removed using a little bit alcohol. Do not use organic solvents for cleansing.
5. Store the instrument in a cool, dry environment. Cover the microscope with the
dust cover when not in use.
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1 Components Illustration
1
Eyepiece
9
Microscope Base
17
Brightness Intensity Dial
2
Diopter Ring
10
Viewing Head
18
Power Switch
3
Eyepiece Tube
11
Head Thumb Lock Screw
19
Focus Stop Lever
4
Nosepiece
12
Microscope Body
20
Condenser Lock Thumb Knob
5
Objective
13
Focus Tension Ring
21
Condenser Focus Knob
6
Slide Holder
14
Coarse Focus Knob
22
Abbe Condenser
7
Mechanical Stage
15
Fine Focus Knob
23
Color Filter Holder
8
Light Collector
16
X-Y Stage Moving Knobs
24
Iris Diaphragm Lever
1 2 3
4
5 6 7
8
9
10
11
12
13
14
15
16
17
18
19
21
22
23
24
20
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2 Installation
2.1 Installation of the binocular viewing head
1) Loosen the head thumb lock screw on the top of the microscope body and remove
the plastic cover on the top.
2) Remove the cap on the dovetail of the binocular viewing head.
3) Seat the dovetail completely of the viewing head into the socket on the top of the
microscope body and tighten the head thumb lock screw.
Caution:
Do not release the viewing head from your hand grip until you are sure the viewing
head is installed securely.
2.2 Installation of the eyepieces
1) Remove the protective caps from the eyepiece tubes.
2) Insert the eyepieces into the eyepiece tubes.
2.3 Installation of the objectives
1) Adjust the coarse focus knob until the mechanical stage is at its lowest position.
2) Turn the caps counter-clockwise to remove them from the nosepiece.
3) Take the objectives out from the plastic cases and turn each one clock-wise into the
holes on the nosepiece. Install the 4X objective into the nosepiece first. Then in a
counter-clockwise direction, rotate the nosepiece and install each succeeding higher
magnification objective as shown in Fig. 1.
Note:
Inspect the objectives frequently for dirt or oil; clean if necessary.
Use the 10X objective to initially focus the image of your specimen.
When changing the objective magnification, rotate the objective nosepiece until
you hear a “click” sound or have a clear "in position" feeling. This ensures the
objective is centered in the optical light path.
2.4 Installation of the glass filter
1) Swing out the color filter holder under the condenser.
2) Place the filter into the holder as shown in Fig. 2, swing the holder in.
Fig.1
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Fuse
Fuse Holder
2.5 Replacing the fuse
1) Turn off the power switch and disconnect the power cord.
2) Turn over the microscope on its side; find the fuse holder at the bottom of the base.
3) Turn the fuse holder counter-clockwise to take it off, replace the fuse, and then turn
it on clockwise. See Fig. 3.
Caution:
Before you turn over the microscope, be sure to take the eyepieces off and be certain
that the head is securely locked by the thumb screw.
2.6 Installing the mirror (optional, may not included in your package)
1) Turn off the power switch and disconnect the power cord.
2) Screw off the light collector on the microscope base.
3) Screw the black disc onto the base and then insert the mirror into the hole at the
center of the black disc. See Fig. 4. You may try to get reflected ambient light on
either side of the mirror with different angles for best result.
Fig.3
Fig.4
Fig.2
Color Filter
Color Filter Holder
Mirror
Light Collector
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Note:
The mirror is only used when there is a power failure or you are in the field and no
power is available.
2.7 Installation of the camera (optional, may not included in your package)
1) Take off the eyepiece from the eyepiece tube.
2) Insert the camera into the eyepiece tube, and then connect
the camera to a computer via USB2.0 cable. See Fig.5.
3) The manual for the camera is on a CD (or mini CD). Refer to
the manual to install the driver and software on to the
computer.
4) The camera is optional and may have different color and
shape from the one in the figure, depending on the model
purchased.
2.8 Installation of the darkfield condensers (optional, may not included in your package)
Please see section 7 (Darkfield Condenser Installation and Operation Instructions)
3 Operation
3.1 Adjusting illumination
1) Plug the power cord into the power socket on the microscope and connect it to the
power outlet.
2) Turn on the power switch.
3) Rotate the brightness intensity dial to increase or decrease the brightness.
Caution:
A diffusion filter is attached beneath the condenser to get uniform light and protect
your eyes from strong light when a low power objective applied. The diffusion filter
can be swung out to make the view field brighter when observing with a high power
objective, such as 100X objective.
3.2 Placing specimen
1) Place the slide on the mechanical stage.
2) Use the slide holder to gently secure the slide.
3) Turn the X and Y stage moving knobs to position the specimen in the center of
viewing field.
Caution:
Be sure not to allow an objective to touch a specimen slide when changing objectives.
3.3 Adjusting interpupillary distance
While observing with both eyes, hold the left and right eyepiece tubes then slide the
tubes in and out. The interpupillary distance is correct when the left and right fields of
view converge completely into one image.
Fig.5
Camera
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3.4 Adjusting eyepiece diopter
1) Rotate the 10X objective into position.
2) Rotate the diopter rings on the eyepiece tubes
until its numerical value is the same as your
interpupillary distance, for example, 70 in the
figure (See Fig. 6).
3) Close your left eye and bring the specimen
into focus following the focusing procedures in
3.5.
4) Close your right eye and bring the same
specimen into clear sharp focus by adjusting
the diopter ring on left eyepiece tube only. Do
not use focus knobs at this step.
5) Since both sides are adjustable, you may also
do the above in the opposite way, in other
words, left eye first and right eye second.
3.5 Focusing
1) With the 10X objective in position, raise the mechanical stage using the coarse
focus knob until the specimen is close to the objective.
2) Turn the coarse focus knob until the specimen is in focus.
3) Use the fine focus knob to obtain a sharp image.
4) To get a good focused image, you may need to combine the focus knob adjustment
and interpupillary distance adjustment, along with eyepiece diopter adjustment
stated in 3.3 and 3.4.
5) You may now switch to another magnification objective.
Tips:
To prevent your specimen slide from making contact
with an objective, raise the stage to its highest position
without contacting the 100X objective, then tighten the
focus stop lever (Fig. 7). Give the stage a tiny extra
moving space to ensure the objective can be focused
every time.
3.6 Applying the immersion oil
1) Rotate the objective nosepiece to seat the observing position between the 40X and
100X objectives as shown in Fig. 8 (a).
2) Place a drop of immersion oil on the slide cover as shown in Fig. 8 (b).
3) Rotate the objective nosepiece to seat the 100X objective to the observing position
until you hear a “click” sound.
4) After observing the specimen, use the camera cleaning kit to clean the 100X
objective lens gently and the specimen in time.
5) If it is hard to clean, you need a little bit alcohol to clean the 100X objective lens and
the specimen.
Fig.6
Fig.7
70
Focus Stop Lever
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(a)
Caution (important):
When you use the 100X objective to observe the specimen, you have to finish
observing the specimen with the 4X, 10X, 40X objectives.
When you use the 100X objective to observe the specimen, you have to apply the
immersion oil on the top of the slide cover.
When you apply the immersion oil with the 100X objective, do not let the immersion
oil to contact with the dry objective lenses (especially the 40X). If the immersion oil is
on the dry objectives lens, please use the camera cleaning kit to clean the objectives
lens in time. The oil will damage the dry objective lenses.
After observing the specimen with the 100X objective, clean the 100X objective lens
first.
3.7 Adjusting condenser
1) Turn the condenser focus knob to raise or lower the condenser.
2) Raise the condenser when using high power objectives and lower it when using low
power objectives.
Note:
The centering of the condenser and the light axis of the objective are factory adjusted.
Do not attempt to re-adjust.
The highest position of the condenser has been factory adjusted. Do not attempt to
re-adjust.
3.8 Adjusting iris aperture diaphragm
Swing the iris diaphragm lever (Fig. 9) left or right to
adjust the aperture size.
Note:
The iris diaphragm is designed to adjust the aperture
size, not to adjust the brightness although the
brightness will be changed when it's adjusted. When
aperture is adjusted to smaller size, the contrast will be
increased and the depth of field will be increased as
well. Turn up the intensity of the light if the image is too
dim.
Fig.8
Iris Diaphragm Lever
Fig.9
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3.9 Adjusting focus tension
The focus tension has been pre-set at the
factory. If the mechanical stage drops by itself,
rotate the tension adjustment ring (Fig. 10)
situated between the coarse focus knob and
microscope body on the power switch side until
the tension is in maintained.
3.10 Camera (optional, may not included in your
package)
1) Bring the microscope into focus by following the
procedures in 3.5.
2) Install the camera by following the procedures in 2.7.
3) Open image observing sofeware to examine (more details see camera’s manual).
4) You also can capture images or record live videos through the software, depending
on the functions provided by the software.
Note:
Please refer to the manuals in the camera’s CD for the details of installation and
operation of the camera.
3.11 Darkfield condensers observation (optional, may not included in your package)
Please see section 7 (Darkfield Condenser Installation and Operation Instructions)
Focus Tension Ring
Fig.10
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4 Specifications
Model
M827TL series
Total Magnification
40X, 64X, 100X, 160X, 400X, 640X, 1000X, 1600X
Viewing Head
Binocular, 45º inclined, 360º swiveling
Interpupillary Distance
Sliding adjustment, 2-3/16" ~ 2-15/16" (55mm~75mm)
Diopter Adjustment
On both eyepiece tubes
Eyepieces
1 pair of WF10X/18
1 pair of WF16X
Objective Tube Length
160mm
Nosepiece
Revolving quadruple
Objectives
Achromatic DIN 4X, 10X, 40X(spring), 100X(spring, oil)
Condenser
Abbe, NA=1.25, with iris diaphragm and filter holder
Rack and pinion adjustment
Focus Mechanism
Coaxial coarse and fine focusing knobs on both sides with
focus stop
Minimum fine focusing adjustment at 0.002mm, range 28mm
Mechanical Stage
Double layer, dimension: 5-1/2” x 5-1/2” (140mm x 140mm)
Translational range: 3” x 2” (75mm x 50mm)
Cameras (optional)
Refer to the cameras specifications
Darkfield Condensers (optional)
Refer to the darkfield condensers specifications
Carrying Case (optional)
Refer to the carrying case specifications
Illumination
Transmitted, replaceable 3W LED light, variable intensity
Power Supply
AC 100V-240V, 50/60HZ (US and Canada plug)
Dimension
7-7/8” x 10-1/4” x 15-3/8” (20cm x 26cm x 39 cm)
Net weight
10 lb (4.5 kg)
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5 Optional Parts
(The optional parts may be included in some models or sold separately.)
1) Cameras
Model
Resolution
Operating System
Software
A1502
640 x 480 (0.3MP)
MS Windows
Mac OS X 10.6-10.8
Included
A3513U
1280 x 1024 (1.3MP)
MS Windows
Mac OS 10.8 and up
Linux (2.6 or above)
A3520U
1600 x 1200 (2.0MP)
A3530U
2048 x 1536 (3.0MP)
A3550U
2592 x 1944 (5.0MP)
A3590U
3488x2616 (9.0MP)
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2) Darkfield Condensers
Model
Darkfield
Condenser
Numerical
Aperture
Objective
Mounting
Size(diameter)
A191
Dry
0.7-0.9
-
37mm
A191BD
Oil
1.36-1.25
-
37mm
A191BOIL
Oil
1.36-1.25
Plan 100X/1.25 oil-0.5
160/0.17(spring), w/ iris
diaphragm
37mm
3) Carrying Case
Model
Dimension
Net Weight
A187V
9-7/8" x 11" x 18-1/2"
(25cm x 28cm x 47cm)
2 lb 6 oz (1.1 kg)
A185A
18-1/8" x 13" x 13-3/8"
46cm x 33cm x 34cm
10 lb 14 oz (4.93 kg)
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6 Troubleshooting Guide
GENERAL PROBLEMS
Problem
Cause
Solution
Lamp does not light
when switched on
No electrical power
Check power cord connection
LED or power unit dead
Replace LED light
Fuse blown out
Replace fuse
Darkness at the
periphery or uneven
brightness in the field
of view
Revolving nosepiece not in click stop
position
Revolve the nosepiece to click-stop
position by swinging the objective
correctly into the optical path
The light source of the lamp is not at
the center
Adjust the position of the lamp
Dirt or dust on the
view
Dirt or dust on the lens eyepiece,
condenser, objective, collector lens or
specimen
Clean the lens with a camera cleaning kit
Poor image quality or
not able to get
focused image
No slide cover attached to the slide
Attach a 0.17mm slide cover
Slide cover is too thick or thin
Use a slide cover of the appropriate
thickness (0.17mm)
Slide may be upside down (specimen
at the bottom)
Turn slide over so the cover-glass faces
up
Diopter adjustment is not set properly
Readjust the diopter settings
Immersion oil is on a dry objective
(especially the 40X)
Check the objectives, clean if necessary
No immersion oil used with 100X
objective
Use immersion oil
Air bubbles in immersion oil
Remove bubbles
Condenser aperture is closed or open
too much
Open or close properly
Condenser is positioned too low
Position the condenser upward
Specimen rises from stage surface
Secure the specimen in the slide holder
Blue filter not used
Use daylight blue filter
Lamp intensity is too high or low
Adjust the light intensity by rotating the
intensity control dial
Slippage of focus
when using the
coarse focusing knob
Fine focus is
ineffective
Tension adjustment is set too low
Increase the tension on the focusing
knobs
Tension adjustment is set too high
Loosen the tension on the focusing
knobs
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DARKFIELD PROBLEMS
Problem
Cause
Solution
It’s totally dark in the
viewing field
The light is not on
Turn on the light
(A191BOIL, A191BD only) There is no
oil in between the condenser top
lens and slide
Place a drop of oil on the top lens of
condenser and let it contact the underside
of slide
(A191BOIL only) The built-in iris
diaphragm of 100X objective is not
in proper position
Adjust the iris diaphragm ring on the
objective
The condenser is not in the right
position
Lower or raise the condenser slightly to the
position that the specimen is the brightest.
(A191BOIL, A191BD only) Make sure the oil
contact the bottom of the slide and the top
of the condenser during the adjustment.
The illumination is
insufficient or too
bright
The intensity of lamp is too low
Adjust the intensity dial to increase the
brightness
The condenser is not in the right
position
Lower or raise the condenser slightly to the
position that the specimen is the brightest.
(A191BOIL, A191BD only) Make sure the oil
contact the bottom of the slide and the top
of the condenser during the adjustment.
The condenser is not centered
properly
Adjust the translational centering screws to
center the condenser
(A191BOIL only) The built-in iris
diaphragm of 100X objective is not
in proper position
Turn the iris diaphragm ring on the
objective
Image of the
specimen is not
clear and lacking in
sufficient contrast
The specimen is not suitable for
darkfield observation
Change to brightfield or phase contrast
(A191BOIL only) The built-in iris
diaphragm of 100X objective is not
in proper position
Turn the iris diaphragm ring on the
objective
The condenser is not in the right
position
Lower or raise the condenser slightly to the
position that the specimen is the brightest.
(A191BOIL , A191BD only) Make sure the oil
contact the bottom of the slide and the top
of the condenser during the adjustment.
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7 Darkfield Condenser Installation and Operation
Instructions
7.1 Dry darkfield condenser of A191
1) Mounting the dry darkfield condenser
Loosen the condenser lock thumb screw on the
condenser holder and remove the brightfield
condenser.
Install the dry darkfield condenser and tighten
the condenser lock thumb screw on the
condenser holder (see Fig. 11).
2) Centering the dry darkfield condenser
Turn the 4X objective to the light path.
Turn the condenser focus knob to lower the
condenser till a dark spot showed in the viewing
field as shown in Fig. 12 (a).
Turn the condenser translational centering
screws to move the dark spot to the center as
shown in Fig. 12 (b).
3) Place the slide on the stage.
4) Raise the condenser all the way to the top and lower it a little bit.
5) Following the procedures in this manual to focus and observe.
6) Move the condenser up or down slightly to get the best darkfield viewing.
Note：
The dry darkfield condenser is used with the dry objectives only.
The dry darkfield condenser works with the 4X, 10X, 40X objectives.
The dry darkfield condenser won’t work with the 100X oil immersion objective.
7.2 Oil darkfield condenser of A191BD, A191BOIL
1) Mounting the oil darkfield condensers
Loosen the condenser lock thumb screw on the
condenser holder and remove the brightfield
condenser.
Install the oil darkfield condensers and tighten
the condenser lock thumb screw on the
condenser holder (see Fig. 13).
2) Centering the oil darkfield condensers
Turn the 40X objective to the light path.
Turn the condenser focus knob to slowly lower
and raise the condenser till a dark spot showed in
the viewing field as shown in Fig. 14 (a).
Turn the condenser translational centering
screws to move the dark spot to the center as
shown in Fig. 14 (b).
(a) (b)
Fig.11
Fig.12
Condenser Lock
Thumb Screw
Centering Screws
Condenser Holder
(a) (b)
Fig.13
Fig.14
Condenser Lock
Thumb Screw
Centering Screws
Condenser Holder
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3) When using the 40X objective to observe the specimen
Place the slide on the stage.
Lower the condenser until a sharp brightfield image showing in the viewing field.
Raise the condenser until the darkfield image showing in the viewing field.
4) When using the 100X objective to observe the specimen
Raise the condenser until the top is close to the opening of stage.
Place a drop of immersion oil on the top of condenser.
Place the slide on the stage.
5) Raise the condenser and let the oil drop contact the bottom of the slide. If air
bubbles exist in the oil, clean the oil from the top of the condenser and bottom of
slide with a lens cleaning paper and repeat the procedures.
6) Follow the procedures in this manual to bring the sample on slide in focus and
observe.
7) (A191BOIL Only) When using the 100X oil darkfield objective,
you will need to drop the immersion oil and adjust the iris ring
(Fig. 15) to get proper brightness and contrast of view field.
8) After observing the specimen with 100X objective, use the lens
cleaning paper to gently clean the 100X objective lens, oil
darkfield condenser and the specimen in time.
9) If it is hard to clean, you need a little bit alcohol to clean the 100X
objective lens, oil darkfield condenser and the specimen.
Note：
When using the 40X objective, you won’t need to apply oil drop on the oil
condenser.
When using the 100X objective, the condenser won’t work well if no oil drop
applied on the condenser.
When you apply the immersion oil with the 100X objective, do not let the
immersion oil to contact with the dry objective lenses (especially the 40X). If the
immersion oil is on the dry objectives lens, please use the lens cleaning paper to
clean the objectives lens in time. The oil will damage the dry objective lenses.
After observing the specimen with the 100X objective, clean the 100X objective
lens first.
Iris Ring
Fig.15
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