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User Manual
Binocular Biological
Microscope
Model M829
MicroscopeNet.com
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Table of Contents
i. Caution……..……………………………………………………………1
ii. Care and Maintenance……….………………………………….….…2
1. Installation ……………………………………….…………………..…3
2. Operation……………………….………………………….…………....5
3. Specifications…………………….………………………………….....7
4. Troubleshooting Guide…..………………………………………........8
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i. Caution
1. Find the “UP” sign and place the Styrofoam container on your table or bench so that
the arrow is pointing upward. Open the shipping carton carefully to prevent any
accessory items (i.e. objectives or eyepieces) from dropping and being damaged.
2. Do not discard the molded Styrofoam container. The container should be retained
should the microscope ever requires reshipment.
3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty
environments. Ensure that the microscope is located on a smooth, level and firm
surface.
4. If any specimen solutions or other liquids splash onto the stage, objective or any
other component, disconnect the power cord immediately and wipe up the spillage.
Otherwise, the instrument may be damaged.
5. Important: the lamp, lamp housing and adjacent parts will become very hot. Do not
touch these parts until they have completely cooled. Never attempt to handle a hot
halogen bulb.
6. All electrical connectors (power cord) should be inserted into an electrical surge
suppressor to prevent damage due to voltage fluctuations.
7. For safety when replacing the halogen lamp or fuse, be sure the main switch is off,
unplug the power cord, and only replace the halogen bulb after the bulb and the lamp
house has completely cooled.
8. Confirm that the input voltage indicated on your microscope corresponds to your line
voltage. The use of a different input voltage other than that as indicated will cause
severe damage to the microscope.
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ii. Care and Maintenance
1. Do not attempt to disassemble any component including eyepieces, objectives or
focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on
metal surfaces should be cleaned with a damp cloth. More persistent dirt should be
removed using a mild soap solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using an
air stream from an air bulb. If dirt remains on the optical surface, use a soft cloth or
cotton swab dampened with a lens cleaning solution (available at camera stores). All
optical lenses should be swabbed using a circular motion. A small amount of
absorbent cotton wound on the end of a tapered stick makes a useful tool for
cleaning recessed optical surfaces. Avoid using an excessive amount of solvents as
this may cause problems with optical coatings or cemented optics or the flowing
solvent may pick up grease making cleaning more difficult. Oil immersion objectives
should be cleaned immediately after use by removing the oil with lens tissue or a
clean, soft cloth.
Store the instrument in a cool, dry environment. Cover the microscope with the dust
4.
cover when not in use.
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1. Installation
1.1 Installation of the binocular viewing head
1) Loosen the thumb screw on the head of the body and remove the plastic cover on the
top.
2) Remove the cap on the dovetail of the binocular viewing head.
3) Insert the binocular viewing head into the head of the body; ensure that the dovetail
is completely seated into the socket.
4) Tighten the thumb screw. Do not release the head from your hand grip until you are
sure the head is installed securely.
1.2 Installation of the eyepieces
1) Remove the protective caps from the eyepiece tubes.
2) Insert the eyepieces into the eyepiece tubes.
1.3 Installation of the objectives
1) Adjust the coarse focus knob until the mechanical stage is at its lowest position.
2) Install the 4x objective into the nosepiece. Then in a clock-wise direction, rotate the
nosepiece and install each succeeding higher magnification objective.
Note:
Inspect the objectives frequently for dirt or oil; clean if necessary.
Use the 10x objective to initially focus the image of your specimen.
When changing the objective magnification, rotate the objective nosepiece until you
hear a “click” sound. This ensures the objective is centered in the optical light path.
1.4 Installation of the glass filter
1) Swing out the filter holder under the condenser.
2) Insert the filter into the holder, swing the holder back in.
1.5 Replacing the fuse
1) Turn off the power and disconnect the power cord.
2) Turn the fuse holder counter-clockwise to take it off.
3) Insert new fuse, and then turn it back on clockwise.
Caution: The fuse is located at the bottom of the base. Before you turn over the
microscope, be sure to take the eyepieces off and be certain that the head is securely
locked by the thumb screw.
1.6 Installing (or changing) the halogen bulb
1) Turn the power off and disconnect the power cord. Allow some time to cool down the
lamp.
2) Open the cover on the bottom of the base by loosening the thumb screw.
3) Take out the dead bulb and insert the new bulb. Be sure the pins on the bulb are
completely inserted into the lamp socket.
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4) You may also loosen the two screws on the cover to adjust the position of the bulb to
get centered and even brightness.
5) Screw the cover back on.
Caution: Before you turn over the microscope, be sure to take the eyepieces off and be
certain that the head is securely locked by the thumb screw.
1.7 Installing the mirror (optional, your model may not have one)
1) Turn the power off and unplug the power cord.
2) Screw off the light cover on the base.
3) Thread the black disc onto the base and then insert the mirror into the hole at the
center of the black disc.
4) You may try to get reflected ambient light on either side of the mirror with different
angles for best result.
Note: The mirror is only used when there is a power failure or you are in the field and
no power is available.
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2. Operation
2.1 Adjusting illumination
1) Connect the power cord and turn on the main switch.
2) Rotate the variable intensity dial to increase or decrease the brightness.
2.2 Placing specimen
1) Place the slide on the mechanical stage.
2) Use the slide holder to gently secure the slide.
3) Turn the X and Y translational adjustment knobs to position the specimen for viewing.
Caution: Be sure not to allow an objective to touch a specimen slide when changing
objectives.
2.3 Focusing
1) With the 10x objective in position, raise the mechanical stage using the coarse focus
knob until the specimen is close to the objective.
2) Turn the coarse focus knob until the specimen is in focus.
3) Then use the fine focus knob to obtain a sharp image.
4) You may now switch to another magnification objective.
Tips: To prevent your specimen slide from making contact with an objective, raise the
stage to its highest position without contacting the 100x objective, then tighten the upper
limit mechanical stage lever.
2.4 Adjusting condenser
1) Turn the condenser focus knob to raise or lower the condenser.
2) The condenser is raised when using high magnification objectives and lowered when
using low magnification objectives.
Note:
The centering of the condenser and the light axis of the objective are factory
adjusted. Do not attempt re-adjustment.
The highest position of the condenser has been factory adjusted. Do not attempt
re-adjustment.
2.5 Adjusting iris diaphragm
Move the Iris Diaphragm Lever left or right to adjust the aperture size.
Note: The iris diaphragm is designed to adjust the aperture size, not to adjust
brightness. Generally, opening the diaphragm to 70-80% of the N.A. value of the
respective objective will provide an image of acceptable quality. If you want to observe
the image of the iris diaphragm, remove one eyepiece and look through the tube. You
will see a dark circle encroaching on the bottom of the tube.
2.6 Adjusting interpupillary distance
While observing with both eyes, hold the left and right eye tubes then slowly slide the
tubes in and out. The interpupillary distance is correct when the left and right fields of
view converge completely into one image.
2.7 Adjusting eyepiece diopter
1) Using the 10x objective and your right eye only, observe your specimen through the
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eyepiece and bring it into focus by adjusting the focus knobs.
2) Then observe the specimen with your left eye only through the left eyepiece. If the
specimen is not in focus, rotate the diopter ring until a sharp image is obtained.
3) Since both sides are adjustable, you may also do the above in the opposite way, in
other words, left eye first and right eye second.
2.8 Adjusting tension knob
The tightness of the tension adjustment collar has been pre-set at the factory. If the
mechanical stage drops by itself, rotate the tension adjustment collar located inside the
focus knob on the power switch side until the tension is in maintained.
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3. Specifications
General
Objective Tube Length 160mm
Viewing Head Binocular, inclined 30º, swiveling 360º, interpupillary distance
55-75mm, adjustable diopter
Eyepieces WF10X, P16X
Nosepiece Quadruple
Objectives Achromatic 4X, 10X, 40X(spring), 100X(spring, oil)
Focus system Coaxial coarse and fine focusing, minimum fine focusing
adjustment at 0.002mm, range 28mm
Condenser Abbe, NA=1.25
Stage Double layer mechanical stage, area 140mmx140mm,
movement range 75mmx50mm
Illumination Halogen lamp 6V/20W
Eyepieces
Designation Magnification Field of View Focal Length
Wide Field 10X 18mm 24.94mm
Plan Field 16X 11mm 15.58mm
Objectives
Magnification
4X 0.10 0.17mm 31.04mm 37.5mm Dry
10X 0.25 0.17mm 17.13mm 7.316mm Dry
40X(S) 0.65 0.17mm 4.65mm 0.632mm Dry
100X(S, Oil) 1.25 0.17mm 2.906mm 0.198mm Oil
Numerical
Aperture
Thickness of
slide cover
Focus
Working
Distance
Working
Mode
Magnifications
Eyepiece 10X 16X 10X 16X 10X 16X 10X 16X
Objective 4X 10X 40X 100X
Magnification 40X 64X 100X 160X 400X 640X 1000X 1600X
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4. Troubleshooting Guide
OPTICAL PROBLEMS
Problem Cause Solution
Darkness at the
periphery or uneven
brightness in the
field of view
Dirt or dust on the
view
Poor image quality
Revolving nosepiece not in click
stop position
The light source of the bulb is not
at the center
Dirt or dust on the lens eyepiece,
condenser, objective, collector
lens or specimen
No slide cover attached to the
slide
Slide cover is too thick or thin
Slide may be upside down
Immersion oil is on a dry objective
(especially the 40x)
No immersion oil used with 100x
objective
Revolve the nosepiece to click-stop
position by swinging the objective
correctly into the optical path
Adjust the position of the bulb
Clean the lens with a camera
cleaning kit
Attach a 0.17mm slide cover
Use a slide cover of the appropriate
thickness (0.17mm)
Turn slide over so the cover-glass
faces up
Check the objectives, clean if
necessary
Use immersion oil
Lamp does not light
when switched on
Air bubbles in immersion oil Remove bubbles
Condenser aperture is closed or
open too much
Condenser is positioned too low Position the condenser upward
ELECTRICAL PROBLEMS
Problem Cause Solution
No electrical power Check power cord connection
Lamp bulb burnt out Replace bulb
Fuse blown out Replace fuse
Open or close properly
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IMAGE PROBLEMS
Problem Cause Solution
Specimen rises from stage
Image moves while
focusing
Image tinged yellow
Image is too bright Lamp intensity is too high
Insufficient
brightness
surface
Revolving nosepiece is not in the
click-stop position
Blue filter not used Use daylight blue filter
Lamp intensity is too low
Lamp intensity is too low
Aperture diaphragm closed too far Open to the proper setting
Condenser position too low Position the condenser upward
MECHANICAL PROBLEMS
Secure the specimen in the slide
holder
Revolve the nosepiece to the
click-stop position
Adjust the light intensity by rotating
the intensity control dial
Adjust the light intensity by rotating
the intensity control dial
Adjust the light intensity by rotating
the intensity control dial
Problem Cause Solution
Image will not focus
with high power
objectives
High power objective
contacts slide when
changed from low
power objective
Slippage of focus
when using the
coarse focusing
knob Fine focus is
ineffective
Slide upside down
Cover glass is too thick Use a 0.17mm cover glass
Slide upside down
Cover glass is too thick Use a 0.17mm cover glass
Diopter adjustment is not set
properly
Tension adjustment is set too low
Tension adjustment is set too high
Turn the slide over so the cover
glass faces up
Turn the slide over so the cover
glass faces up
Readjust the diopter settings
Increase the tension on the focusing
knobs
Loosen the tension on the focusing
knobs
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