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User Manual
Binocular Compound
Microscope
Model M825
MicroscopeNet.com
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Table of Contents
i. Caution……..……………………………………………………………1
ii. Care and Maintenance……….………………………………….….…2
1. Component Illustration………………………….…………………..…3
2. Installation ……………………………………………………………...4
3. Operation..…………….……….………………………….…………....5
4. Specifications…………………….………………………………….....7
5. Troubleshooting Guide…................................................................8
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i. Caution
1. Find the “ UP” sign and place the St yrofoam container on your table or bench so that the
arrow is pointing upward. Open the shipping carton carefully to prevent any accessory
items (i.e. objectives or eyepieces) from dropping and being damaged.
2. Do not discard the molded Styrofoam container. The container should be retained
should the microscope ever requires reshipment.
3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty
environments. Ensure that the microscope is located on a smooth, level and firm
surface.
4. If any specimen solutions or other liquids splash onto the stage, objectiv e or any other
component, disconnect the power cord immediately and wipe up the spillage.
Otherwise, the instrument may be damaged.
5. All electrical connectors (power cord) should be inserted into an electrical surge
suppressor to prevent damage due to voltage fluctuations.
6. Confirm that the input voltage indicated on your microscope corresponds to your line
voltage. The use of a different input voltage other than that as indicated will cause
severe damage to the microscope.
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ii. Care and Maintenance
1. Do not attempt to disassemble any component including eyepieces, objectives or
focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on metal
surfaces should be cleaned with a damp cloth. More persistent dirt should be r emoved
using a mild soap solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using an
air stream from an air bulb. If dirt remains on the optical surface, use a soft cloth or
cotton swab dampened with a lens cleaning solution (available at camera stores). All
optical lenses should be swabbed using a circular motion. A small am ount of absorbent
cotton wound on the end of a tapered stick makes a useful tool for cleaning recessed
optical surfaces. Avoid using an excessive amount of solvents as this may cause
problems with optical coatings or cemented optics or the flowing solvent may pick u p
grease making cleaning more difficult. Oil immersion objectives should be cleaned
immediately after use by removing the oil with lens tissue or a clean, soft cloth.
4. Store the instrument in a cool, dry environment. Cover the microscope with the dust
cover when not in use.
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1 Components Illustration
1
2
3
4
9
10
11
12
13
5
6
7
8
1. Eyepiece
2. Eyepiece Tube
3. Diopter Ring
4. Viewing Head
5. Slide Holder
6. Stage
7. Condenser
8. Light Collector
9. Set Screw
10. Nosepiece
11. Objective
12. Stand
14
15
16
17
13. Coarse Focus Knob
14. Fine Focus Knob
15. Translation Knobs
16. Intensity Dial
17. Base
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2 Installation
2.1 Installation of the binocular viewing head
1) Loosen the set screw on the top of the stand and remove the plastic cover on the top.
2) Remove the cap on the dovetail of the binocular viewing head.
3) Seat the dovetail of viewing head into the socket on the top of the stand and tighten
the set screw.
Caution: Do not release the head from your hand grip until you are sure the head is
installed securely.
2.2 Installation of the eyepieces
1) Remove the protective caps from the eyepiece tubes.
2) Insert the eyepieces into the eyepiece tubes.
2.3 Installation of the objectives
1) Adjust the coarse focus knob until the mechanical stage is at its lowest position.
2) Install the 4x objective into the nosepiece. Then in a clock-wise direction, rotate the
nosepiece and install each succeeding higher magnification objective.
Note:
Inspect the objectives frequently for dirt or oil; clean if necessary.
Use the 10X objective to initially focus the image of your specimen.
When changing the objective magnification, rotate the objective nosepiece until you
hear a “click” sound. This ensures the objective is centered in the optical light path.
2.4 Installation of the light filter
1) Swing out the filter holder which is located underneath the condenser.
2) Insert the light filter into the holder, swing the holder back in.
3 Operation
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3.1 Adjusting illumination
1) Insert the power cord into the power socket on the microscope and connect it to the
power outlet.
2) Turn on the main switch at the back of the microscope. 3) Rotate the intensity dial to
increase or decrease the brightness.
3.2 Placing specimen
1) Place the slide on the mechanical stage.
2) Use the slide holder to gently secure the slide.
3) Turn the X and Y translation knobs to position the specimen for viewing.
Caution: Be sure not to allow an objective to touch a specimen slide when chan ging
objectives.
3.3 Focusing
1) With the 10X objective in position, raise the mechanical
stage using the coarse focus knob until the specimen is
close to the objective.
2) Turn the coarse focus knob until the specimen is in focus.
Then use the fine focus knob to obtain a sharp image. You
may now switch to another magnification objectiv e.
Tips:
To prevent your specimen slide from making contact
with an objective, turn the 100X objective in position and
rotate the focus stop lever clock-wise all the way to the
end so that the 100X objective will not contact the
specimen while the stage is adjusted to its highest
position.
3.4 Adjusting interpupillary distance
While observing with both eyes, hold the left and right eyepiece tubes then s wing them
around the center axis. The interpupillary distance is correct when the left and right fields
of view converge completely into one image.
3.5 Adjusting eyepiece diopter
1) Using the 10x objective and your left eye only, observe your specimen through the
eyepiece and bring it into focus by adjusting the focus
knobs.
2) Then observe the specimen with your right eye only
through the right eyepiece. If the specimen is not in focus,
turn the diopter ring on the eyepiece tube until a sharp
image is obtained.
3.6 Adjusting condenser
1) Turn the condenser focus knob to raise or lower the
condenser.
2) The condenser is raised when using high magnification
objectives and lowered when using low magnification
objectives.
Note:
The centering of the condenser and the light axis of the objective are factory adjusted.
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Do not attempt re-adjustment.
The highest position of the condenser has been factory adjusted. Do not attempt to
re-adjustment.
3.7 Adjusting iris aperture diaphragm
Move the iris diaphragm lever left or right to adjust the
aperture size.
Note:
The iris diaphragm is designed to adjust the aperture
size, not to adjust brightness. Generally, opening the
diaphragm to 70-80% of the N.A. value of the
respective objective will provide an image of
acceptable quality. If you want to observe the image of
the iris diaphragm, remove one eyepiece and look
through the tube. You will see a dark circle encroaching
on the bottom of the tube.
3.8
Adjusting focus knob tension
The tightness of the focus knob tension has been
pre-set at the factory. If the mechanical stage
drops by itself, rotate the tension adjustment ring
located inside the focus knob on the power switch
side until the tension is in maintained. Tension
Ring
Tension Ring
4 Specifications
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Model M825
Total Magnification 40X, 64X, 100X, 160X, 400X, 640X, 1000X, 1600X
Objective Tube Length 160mm
30º inclined, 360º swiveling Siedentopf binocular viewing head
Viewing Head
Interpupillary distance 55-75mm Adjustable diopter on right
eyepiece tube
Eyepieces 1 pair of WF10X 1 pair of WF16X
Nosepiece Reversed revolving quadruple
Objectives Achromatic 4X, 10X, 40X(spring), 100X(spring, oil)
Condenser
Focus Mechanism
Stage
Abbe, NA=1.25, w/ iris aperture diaphragm and filter holder
Rack and pinion adjustment
Coaxial coarse and fine focusing knobs on both sides w/ focus
stop, tension adjustable
Double layer mechanical stage Dimension: 5-1/2” x 5-1/2”
(140mmx140mm) Translation range: 3” x 2” (75mm X 50mm)
Illumination Transmitted: 6V/20W Halogen, Variable intensity
Power Supply AC 110V, 60HZ (US and Canada plug)
Dimension 12-1/2” x 11-1/2” x 15-3/4” (32cm x 29cm x 40cm)
Net weight 13 lb (6.34 kg)
5 Troubleshooting Guide
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OPTICAL PROBLEMS
Problem Cause Solution
Darkness at the
periphery or
Revolving nosepiece not in click
stop position
uneven brightness
in the field of view
The bulb of light source is not at
the center
Dirt or dust on the
view
Dirt or dust on the eyepiece,
condenser, objective, collector
lens or specimen
No slide cover attached to the
slide
Slide cover is too thick or thin
Slide may be upside down
Poor image quality Immersion oil is on a dry
objective (especially the 40x)
No immersion oil used with 100x
objective
Air bubbles in immersion oil Remove bubbles
Revolve the nosepiece to click-stop
position by swinging the objective
correctly into the optical path
Adjust the position of the bulb if
possible
Clean the lens with a camera
cleaning kit
Attach a 0.17mm slide cover
Use a slide cover of the appropriate
thickness (0.17mm)
Turn slide over so the cover-glass
faces up
Check the objectives, clean if
necessary
Use immersion oil
Problem Cause Solution
Lamp does not
light when
switched on
Condenser aperture is closed or
open too much
Open or close properly
Condenser is positioned too low Position the condenser upward
ELECTRICAL PROBLEMS
No electrical power Check power cord connection
Lamp bulb burnt out Replace bulb
Fuse blown out Replace fuse
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IMAGE PROBLEMS
Problem Cause Solution
Specimen rises from stage
Image moves
while focusing
surface
Revolving nosepiece is not in
the click-stop position
Blue filter not used Use daylight blue filter
Image tinged
yellow
Lamp intensity is too low
Image is too bright Lamp intensity is too high
Lamp intensity is too low
Insufficient
brightness
Aperture diaphragm closed too
far
Condenser position too low Position the condenser upward
IMAGE PROBLEMS
Problem Cause Solution
Secure the specimen in the slide
holder
Revolve the nosepiece to the
click-stop position
Adjust the light intensity by rotating
the intensity control dial
Adjust the light intensity by rotating
the intensity control dial
Adjust the light intensity by rotating
the intensity control dial
Open to the proper setting
Image will not
focus with high
power objectives
High power
objective contacts
slide when
changed from low
power objective
Slippage of focus
when using the
coarse focusing
knob Fine focus is
ineffective
Slide upside down
Turn the slide over so the cover
glass faces up
Cover glass is too thick Use a 0.17mm cover glass
Slide upside down
Turn the slide over so the cover
glass faces up
Cover glass is too thick Use a 0.17mm cover glass
Diopter adjustment is not set
properly
Tension adjustment is set too
low
Tension adjustment is set too
high
Readjust the diopter settings
Increase the tension on the
focusing knobs
Loosen the tension on the focusing
knobs
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