User Manual
Trinocular Compound
Microscope
Model M834 Series
MicroscopeNet.com
Table of Contents
i. Caution……..……………………………………………………………1
ii. Care and Maintenance……….………………………………….….…2
1. Component Illustration………………………….…………………..…3
2. Installation ……………………………………………………………...4
3. Operation...…………………….………………………….…………....7
4. Specifications…………………….………………………………….....10
5. Optional Parts…………………….………………………….………...11
6. Troubleshooting Guide………………….…………………….………13
7. Darkfield Condenser Installation and Operation Instructions……..15
8. Phase Contrast Kit Installation and Operation Instructions……..…16
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i. Caution
1. Find the “UP” sign and place the Styrofoam container on your table or bench so
that the arrow is pointing upward. Open the shipping carton carefully to prevent
any accessory items (i.e. objectives or eyepieces) from dropping and being
damaged.
2. Do not discard the molded Styrofoam container. The container should be retained
should the microscope ever requires reshipment.
3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty
environments. Ensure that the microscope is located on a smooth, level and firm
surface.
4. If any specimen solutions or other liquids splash onto the stage, objective or any
other component, disconnect the power cord immediately and wipe up the
spillage. Otherwise, the instrument may be damaged.
5. All electrical connectors (power cord) should be inserted into an electrical surge
suppressor to prevent damage due to voltage fluctuations.
6. Important: the lamp, lamphouse and adjacent parts will become very hot during
or short after operation. Do not touch these parts until they have completely
cooled. Never attempt to handle a hot halogen bulb or mercury bulb.
7. Make sure there is sufficient room around the microscope base for heat
elimination.
8. For safety when replacing the lamps or fuses, be sure the main switch is off,
unplug the power cord, and only replace the bulb after the bulb and the
lamphouse has completely cooled.
9. Do not touch the surface of halogen bulbs with bare fingers. If so, clean the
surface with soft cloth.
10. Important: confirm that the input voltage (220V version available, contact seller)
indicated on your microscope corresponds to your line voltage. The use of a
different input voltage other than that as indicated will cause severe damage to the
microscope.
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ii. Care and Maintenance
1. Do not attempt to disassemble any component including eyepieces, objectives or
focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on
metal surfaces should be cleaned with a damp cloth. More persistent dirt should be
removed using a mild soap solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using
an air stream from an air bulb. If dirt remains on the optical surface, use a soft cloth
or cotton swab dampened with a lens cleaning solution (available at camera stores).
All optical lenses should be swabbed using a circular motion. A small amount of
absorbent cotton wound on the end of a tapered stick makes a useful tool for
cleaning recessed optical surfaces. Avoid using an excessive amount of solvents as
this may cause problems with optical coatings or cemented optics or the flowing
solvent may pick up grease making cleaning more difficult. Oil immersion objectives
should be cleaned immediately after use by removing the oil with lens tissue or a
clean, soft cloth.
4. Observe the specimen with the 4X, 10X and 40X objectives in order, then observe
the specimen with the 100X objective. Apply the immersion oil on the slide cover with
the 100X objective. Do not let the immersion oil to contact with the dry objectives
lens (especially the 40X). Clean the dry objective lens using the lens cleaning paper
if the immersion oil is on the dry objectives lens. Clean the 100X objective lens first
using the lens cleaning paper after observing the specimen with the 100X objective,
then clean the specimen. More persistent dirt should be removed using a little bit
alcohol. Do not use organic solvents for cleansing.
5. Store the instrument in a cool, dry environment. Cover the microscope with the dust
cover when not in use.
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1. Components Illustration
1
Photo Tube
9
Fine Focus Knob
17
Mechanical Stage
2
Viewing Head
10
Power Switch
18
Condenser
3
Microscope Body
11
Microscope Base
19
Condenser Holder
4
Slide Holder
12
Eyepiece
20
X-Y Stage Moving Knobs
5
Condenser Lock Thumb Screw
13
Diopter Adjusting Ring
21
Light Collector
6
Condenser Focus Knob
14
Eyepiece Tube
22
Brightness Intensity Dial
7
Stage Upward Stopper
15
Nosepiece
8
Coarse Focus Knob
16
Objectives
2 4 5 6 7 8 9 1 3
13
15
16
17
18
19
20
21
12
14
10
11
22
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2. Installation
2.1 Installation of the trinocular viewing head (Fig.1)
1) Loosen the set screw on the top of the microscope
body and remove the plastic cover.
2) Remove the cap on the dovetail of the trinocular
viewing head.
3) Seat the dovetail of trinocular viewing head into the
socket on the top of microscope body completely and
tighten the set screw.
Caution:
Do not release the viewing head from your hand grip until you are sure the viewing
head is installed securely.
2.2 Installation of the photo tube
1) Remove the cap on the top of the viewing head.
2) Thread the photo tube into the top of the viewing head.
2.3 Installation of the eyepieces
1) Remove the protective caps from the eyepiece tubes.
2) Insert the eyepieces into the eyepiece tubes.
2.4 Installation of the objectives
1) Adjust the coarse focus knob until the mechanical stage is at its lowest position.
2) Turn the caps counter-clockwise to remove them from the nosepiece.
3) Take the objectives out from the plastic cases and turn each one clock-wise into the
holes on the nosepiece. Install the 4X objective into the nosepiece first. Then in a
counter-clockwise direction, rotate the nosepiece and install each succeeding higher
magnification objective as shown in Fig. 2.
Note:
Inspect the objectives frequently for dirt or oil; clean if necessary.
Use the 10X objective to initially focus the image of your specimen.
When changing the objective magnification, rotate the objective nosepiece until
you hear a “click” sound or have a clear “in position” feeling. This ensures the
objective is centered in the optical light path.
Fig. 1
Set Screw
Fig.2
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2.5 Installation of the glass color filter
1) Swing out the filter holder under the condenser.
2) Insert the filter into the holder as shown in Fig. 3, swing the holder in.
2.6 Installing (or changing) the halogen bulb
1) Turn the power off and disconnect the power cord.
2) Allow some time to cool down the lamp and light housing.
3) Take the eyepieces off and check if the view head is securely locked by the set
screw. Put the microscope down on its back gently.
4) Open the cover on the bottom of the base by pull the knob. Take out the dead bulb
and insert the new bulb. Be sure the pins on the bulb are completely inserted into the
lamp socket. See Fig. 4
5) Close the cover.
Caution:
Don’t touch the Halogen bulb with your bare fingers. The fingerprints left on the bulb
will shorten the bulb’s life.
Fig. 3
Fig. 4
Color Filter
Color Filter Holder
Knob
Bulb
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