Olympus BH2 MJLT, BH2 MJLA User manual [gb]

IC INSPECTION MICROSCOPE

1-

This instruction manual has been prepared for use with the Olympus

IC Inspection Microscope Models
putting the microscope into operation, it is recommended that you
read this manual carefully and familiarize yourself with the use of this
microscope in order to obtain the best performance.

WARNING

BH3-MJLT

and BH3-MJLA. Before

OLVMPUS

_.I_-

_

.

_

_-,_

I

;omradl

I.

1.

This microscope is a precision instrument. Always handle it with the care it deserves and avoid abrupt movements or

c.1'

.

on,

-1.L

. .

-

.-

;

;

~.

-.

,,-.:

~

--

--

--

-

.

-...-

--

-

-.

_-

_

;..

_

__

,

,:.

.'

.r.,

"

-.,.--.-..

,

{,,

,,,:

.

L

-.

.

I:

.

,

L..

,.I

.

-

-1

..---

-I

-.---

,'

.--.-

-

.

' , '

1.-

:,

.

'

;-

I

.'c---,--j:

'i,"

.

lA1.'

---. .

.

:

I

---I-----

shock.

2.

Do not subject the microscope to direct sunlight, high temperature and humidity, dust, or excessive vibration.
The operating conditions are: Ambient temperature:

10°C - 35OC (50°F - 95°F)

Relative humidity: 15% - 85%

3.

Prevent dust from accumulating on the lenses and avoid leaving fingerprints on the optics.

4.

Unplug the power cord before replacing the light bulb or fuse.

5. Connect the grounding wire of the microscope.

6.

Use the tension adjustment ring to adjust the rotational tension of the coarse focus adjustment knobs.

7.

Confirm that the line voltage selector switch at the bottom of the microscope base is set to your local mains voltage.

I

Ao~licable Arms

and

Focus Units

I

.

Arm

Focus unit

Stage

8"

BH3-SIC6
BH3-SIC8
BH3-SIC106
BH3-SIC128

*

BH3-SIC10

*

BH3-SIC1410

The items marked with

l~sted above, use of different models of mechanical stages require different models of focus units and horizontal

8. As

0

0

0

0

1

'*'

are scheduled for future sale.

arms. Please consult your local

9.

To power the vertical illuminator and the transmitted light illuminator simultaneously, a power supply unit VH3) for the

+lom

-,

I

0

10

agentldealers for further details on the selection and/or exchange of these models.

F02

0

F04

0

0

0

-

,-

+FOG

0;

0

Halogen lamp housing

(for transmitted

BHS-LSHSO

BH3-LSH50-10

0

0

101

I01

light)

0

0

halogen light source is required.

UV

10. The breath shield and

protective shield mounting pins (paragraph 3-3

)

permit attachment of the shield alternatingly.

The pins have a central tap to accept the clamping screws of the breath shield. Exchange the pins without central tap.

1.

Lens surfaces must always be kept clean. Fine dust on lens surfaces should be wiped off gently with gauze pads.
Carefully wipe off oil or fingerprints on the lens surfaces with a gauze pad moistened with a small amount of
or a

3

:

7

mixture of alcohol and ether.

2.

Do not use organic solutions to wipe the surfaces of various components. Plastic parts, especially, should be cleaned

xylene.

w~th neutral detergent.

3. Fine dust on the half-mirror units of the universal illuminator should be lightly blown off.

If

dust still remains, contact

an Olympus office, as listed on the back page of this manual.

4.

Do not disassemble the microscope for any reason. Only authorized Olympus service personnel should make repairs.

5. When not in use, cover the microscope with the vinyl dust cover provided and keep it out of areas of high heat and
humidity.

1.

STANDARD CONFIGORATIONS

.-

".,

A

-,.-

2.

NOMENCLATURE'

......

-.

.....

;

.

-

---.

3.

ASSEMBLY

_

.

_.

3-1

Assembiy._Outline

3-2

'

Preparation fpr Assembly

3-3

Detailed Assembly Procedure

4.

OPERATION

...........

1

5-1

I I

1

1

I

1

Reflected Light ~rl~htfieldl~arkflelij 0bsedtl.on..

5-2

Reflected

5-3

Refie~ted~Light

5-4

~rensrnitted

_

-'

I._

Light

-

Light

--.

-

.

,..

-

. - --.

:

.

--

......

,

,

,

. %-I.

-

.

,

>

-

,

.

.

.

-

1.

.-

.

-.

'-.

_..--

.

'.

-

.-..

.

.

, ,

_-~-

".

.,

...

.

.

,..

.._.

-.

.

.-

.

Nomarski Differential Interference Contrast Obsepation

Slmp!e,

Observation in Polarlze,d Light

Obsetyatibn

-

--

A

.

-

,

. 7 .

,

L.

..-A

.

....----.

.

,

I--

!:'.

-~.

g

,

..

..

.

.

.

.

--

9

12

13

25

33

34

35,

36.

-

..

1

]

1

1

1

-.

.

1

.5-5

6.

......

Transmitted Light Simple Observation in Polerized.Light

PHOTOMICROGRAPHY

__

_;_._

&_._

.....-

.............

37

1

39.

-

.-

. .

- . -

L

-.i.-

...

..-

--

---

.

-:

The following shows only typical examples of the standard configurations. Check your configuration against the price list,
catalogs, etc.

IC Standard Configurations

Model

BH3-MJ

Observe- Photo- Photo- Observa- Phote Observa- Photo-

tion micro- Observation only micro-

only

Component 163B 863U 1838 ' 1830

BH3-MJW-F

Microscope

Stand

-

Power cord UYCP

Binocular

Tlltlng b~nocular tube BH2-TBI
Tnnocular tube BH2-TR30
Tllt~ng tr~nocular tube

Motorized

nosepiece

Motor~zed

nosepiece

uves

Un~versal vert~cal
lllurn~nator

Br~ghtf~eld half-m~rror unlt BH2-U6F

Darkf~eld half-m~rror unlt BH2-UDF
ND f~lter sl~der. ~nclud~ng 8H2-UND

NO12
Tlnt plate, lnclud~ng BH29TP530

NO12

Polarizer BH2-UP0

Analyzer BH2-UAN

~cof

Breath shleld
Yellow f~lter
Llght balanc~ng f~lter 20LBD3-W

Contrast f~lter 201F550-W

Halogen lamp houslng BH2-ULSH45/50

(50W

Halogen bulb
Halogen lamp hous~ng BH2-HLSH451100

(1

Collector lens BH2-HCL

tube BH2-B130

5

revok~ng BH3-5MRE-M

for

M

objectives

5

revolving

for Neo objec- BH3-5NRE-M

f11ter

and

shutter

frlter

cover

OOWI

BH3-MJLT2-F
BH3-MJ LA4-F
BH3-M JLT4-F

BH2-?lR

BH2-UMA

I

(

BH3-UCO

I

BH3-BSH

1

2OY48-W

45LBD2-N

431F550-W45

IZVSOWHAL-L-L-LI

!

-

-

-

-

I

-

-

I

I

-

-

ll/l!l

1

-

-

-

-

Reflected

W

gnphy

tight

Version Version

BH3-MJLA4 BH3-M J LT2 BH3-MJLT4

-

-

-4,

- -

1

1

1

1

-

-

-

1

-

1

1 1

1
1
1

1

1
1 1

1 1

- -

---

- - -

-

1

-

1

-

-

-

-I-

Ill

-

11-

->

-1-

1

2

I

-

-

1

1

2 2

- -

-

1 1 1

--

-

-

-

-

1

-

--

-

-

graphy

88311

i

--

-

1

1
1

1

I

1

---

I

I

Reflected and Trensm'kted

tion

on!y

1638 86311

rmcw

graphv only

-

-

,

'

-

-

- -

1

-

-

1

-

-

1

1

1

7

-

-

1

-

1

1

-

-

-

1

-

--

1 1 1

1

1

1

-

I

lI-I

1

1/111,

1

-

l

1

/

1
1 111

11

1

-1

-

1

1

-

-

1

1

Light

t~on mcro-

graphy

1830 88311

-1-

-

-I-

1 1

1

1,

1

-

- -

- -

-

-

1

1'1

1

1

-

1

1

I

-

-1

1

-1

I

1

Ill

)I1

-

-

2

1
1

-

-

-I1

L

IC Standard Configurations

Model

Component
Halogen bulb

Halogen lamp hous~ng

(50WI

for

transm~tted l~ght

Halogen bulb JCl 2V5OWHAL-L
Mechan~cal stage wlth

right-hand low drlve
controls

Stage gnp for nght-hand
s~de

Wafer holder plate

6'

and

5'

rlng
Wafer holder plate.

lncludlng
wafer holder rlng

5' x 5'

7'
Nomarsk~ prlsm

attachment

Screwdr~ver
~llen" wrench

Ob~ect'ves

Standard mlrror for
color temperature
determlnat~on

LB eyeplece 10X
LB eyeplece 10X

with

mask
LB photo eyepiece

3 3X

wafer holder

8'

and

mask holder

x

7' mask holder

MSPL

achromatic

achromatic

Nee

achromat~c

universal

verscon

bulk-ln 35tT~n

JC12V1

OOWHAL-L

-

I

BHS-LSH-50

+-

'

BH3SIC6-R 1 1
BH3-SlC8-R
BH3-SGR-R

BH3-WHP6
BH2-MrHR65

6'

BH3-WHPR86

BH3-MH5
BH3-MH7
U-NICS-N

U-NIC 10-N
U-NIC20-N
U-NIC50-N
U-NIC 100-N

AA7213
MA7578
MS

MSPLlOX-T
MS PL20X-T
MS PL5OX
MS PL100X

Neo SPL5X
Neo SPLIOX-T

SPL

Neo SPL2OX-T
Neo SPLlOOX

SPL

Neo SPL5X-NIC
Neo SPLlOX-NIC
Neo SPL20X-NIC
~eo

,

M-SM2

.-

PL5X

SpL50X

SPLSOX-NIC

I

~~~lox

35WHK1 OX

NFK

3 3x

LD

BH3-MJLA2

Observa-

tion
only

1638

-

- -

--

-.

,

-

-

I

-

I

-

-

-

-

-

each

-

-

-

1

I

I

-

-

1-

Reflected Light Version

Photo­micro-

graphy

86311

2

-

-

1

-

--

1

1
1

1

1

-

1

-

1

-

each

1
1

-

-

1

-

each

11-

1 12

1

Reflected end Transmitted Light

BHBMJLA4

Photomicrography

1838

-

-

1830

-

-

Photo­micro-

gmphy

88311

2

-

BH3-MJLTZ

Observa-

tion

only

1638

-

'

1 1

!

I

-

-

2!2

1

1

I

I

I

1

1

-

-

-

1 1
1

-

1

-

I
I
I1

-

-

1

1111

-1-1

*"TI

1

Photo­micro-

graphy

863U

2

1

-

-

Version

BH3-MJLT4

Observa-

tlon

only

183D

-

2

-

-

- -

1;1

Photo­micro-

graph~

883U

2
1

I

2

-

1

1

I

-

I

I

I

I

ekh

-

1

-

-

-

-

I

each

j

-

-

-

-

-

-

1

1

-

-

-

1-

-

1-

each

each

I

'

I

-

-

-

1

1

-

1

1
1

-

-

-

-

1-

each

1

-

1-

each

-

1
1

-

-

-

I

-

lrl

1

j-

-

-I

2

-

-

1-

each

I

--

1,
1

-

1

-

/-

r-

-

*

I

-

-

1-

each

1

I1

1

1

-

-

21

-

-1

each

I

-I-'-

~

I
I

I

each

11

I

I

-

-

-

1

-

1

1
1

1-

1

1

I

IC

Fluorescence Standard Configurations

Model

Component 363F 383F 363F 383F

I

BH3-MJLA2-F

r

Microscope

Power cord UYCP
Trinocular tube BH2-TR30
Tilting trinocular tube BHZ-STR

Motorized

nosepiece for M objectives
Un~versal venical illuminator / BH2-UMA
Brightfield hallf-mirror unit BH2-UBFL

ND05 filter

Roof cover
Breath shield
Light balancing fllter
Fluorescence supplementary

unit consisting of slider for
supplementary exciter filter.
adapter to fit extension tube 8H2-URF

BH3-UET8 with BH2-UMA,
supporting block and UV
protective shield

Extension tube BH3-UET8

Stand

-

5 revolving BH3-5MRE-M

BH3-MJLA4-F
BH3-MJLT2-F
BH3-MJLT4-F

--A

I

I

BH2-UCO

/

BH3-BSH

I

45BD2-N

I

1

-

BH3-MJLA2 BH3-MJLA4 BH3-MJLT2 BH3-MJT4 BH3-MJL

1

-

-

-

1

-

-

.

-

-

-

-

2

-

.

.

-

-.

1 1

--

1

1

1 1

1

1 1 1 1
1

1

-

1

--

1

-

1

1

-

1

-

1

1

-

1 1

1

1 1

-

1

1 1

1

1

I

lI1-

ll11-

1

1-11 1

I

-

I

I

I

1

i

I

F

L-SET

-

-

-

1

-

-

-

-

1

1

I

-

1

IC Fluorescence Standard Configurations

BH3-MJLAZ BH3-MJLA4 BH3-MJLT2 BH3-MJT4 BH3-MJL

Component

Fluorescence lamp hous~ng

-

-

Mercury burner

---

-

Power supply
Center~ng screen
Halogen lamp houslng

(for transm~tted I~ght)
Halogen bulb

--

Mechan~cal stage w~th
r~ght-hand low dr~ve controls

Stage grtp for nght-hand s~de

Wafer holder plate

6'

and

5'

wafer holder rlng

Wafer holder plate, ~ncluding

8'

and

6'

wafer holder nng

5'

x

5'

mask holder

7'

x

7'

mask holder

,

ob~ectlve

1

LB

eyeplece

LB

eyeplece

In

35

LB

photo eyeplece

1 OX

mm mask

(50W) BHS-LSH-50

MS PL

achrornatc

10X WHKlOX

mth

bullt-

3 3X NFK 3.3X

1

BH2-LSRF

-

USHlO2D

-­BH2-RFL-T3

.

-

BH2-SGRF

JC

I

ZVSOWHAL-L

BH3-SICSR
BH3-SIC8-R
BH3-SGR-R
BH3-WHP6
BH3-WHR65
BH3-WHPR86

BH3-MH5
BH3-MH7

MS

PL5X

MS

PLlOX

MS

PL2OX

MS

PL50X

MS PLlOOX

35WHK1 OX

LO

-~

363F

I

1

--

1

1

--

-

-

1

-

1
1

--

1

-

1

I--

1-each 1-each

1

11/

1

383F

-

I

-

-

I--

1

1

-

-

1

1

1

--A

-

-

-1,-

A

-

363F 383F FL-SET

1

­1 1

:

2

-

,-A

lI-1-

-

1
1

1

*

I

-

1

-each

1

-

-

1

-each

-

2

1

-

1

1

1

1

-

-

-

-

-

-

-

-

-

-

1

-

1

1

1

1

1

-

LCD

Standard Configurations

Component

1

Microscope

Power cord

--

--

Tlltlng

Motorized

noseplece for M objectlves

Motorized

nosep~ece for 5
Un~versal venlcal ~llum~nator BH2-UMA
Br~ghtf~eld half-m~rror unlt I BH2-UBF
Darkf~eld half-rn~rror unlt
Polanzer BH2-UP0

Tint

plate. ~nclud~ng
Polar~zor for lransm~rred
Rotatable analyzer BH3-UAN360
Llght

Roof cover
Breath sh~eld
Halogen lamp

for transm~tted llght

Halogen bulb
Halogen lamp
Halogen bulb

Collector lens

Stana

--

--

binocular

5

revolving

-

revolving

Neo object~ves

balancing

housing

housrng

tube

ND12

light

fllter 20LBD3-W

(50W

(100W

BH3-MJLT4-F
BH3-MJLT4S-F

1

UYCP

I

BH2-TBI
BH3-SMRE-M

BH3-5NRE-M

I

BH2-UDF

f~lter

BH2-UTP530

BH3-POL

,

45LBD2-N

,

BH3-UCO
BH3-BSH
BHS-LSH-50

BH3-LSH50-10

JC12V!XWHAL-L

BH2-HLSH45/100

I

JClZVlOOWHAL-L

BHZ-HCL

\

Model

-

BH3-MJLT4

-033

U

1

-

--

---.-a

1

1

-

-

A-

1
1

1

-.,-

1
1
1
1
1

--

--

1
1
1
1
1

-

2

1

22,2]2

1

BHSMJLT4

-1

B

1

-

--

-

-

-

1

--

1

--

1

-- - -

-

1

-11

,--.

-

-

1

1

1

-

1
1
1

--.-.

1

-

.

--

2
1

1

06

U

1

-

-

-

-

1

--

-

--

1

---

-

-++-j.f]IT

1

j1;

!lI1l!

1

l!-

1

-

1

-.

1

1
1
1
1

-

2

1

1

BHSMJLT4

B

1

-

1
1

:-I1

1-

1

lil
1

1
1
1
1

-

2

111

1

-1

28

I

BWLT4SBH3MJLT4S

-1 01

U

1

-

1
1

U

-

7'

1

R-~

17

111

lI1

1

111
1

1

1
1
1
1

-11

1

1

1

1
1
1

-

212

1

I

?I2

1

111

-141

U

-

1-

--

1

-'

1
1

1

I

1

1

-

1
1
1

-

1

2

1

LCD

Standard Configurations

Component

t

LWD MSPL100X-LCD

Neo SPL100X-NIC

ULWD

Neo

SPLlOOX

I

U-NIC

Nomarskl

Screwdrcver
Allen" wrench
LB eyepiece 1 OX WHKlOX

Mechanical

r~ght-hand low drlve

controls BH3-SIC106-R

set

U-NIC set

stage wlth

U-NIC10-N 1
U-NICUL2O-N

U-NICUL50-N
U-NICUL80-N

AA7213 1

MA7578 1 2

BH3-SIC8-R 1

Model

BH3-MJLT4

-088

-

--

2

BH3-MJLT4 BH3-MJLT4

-1

06

U

I----

-1

28

B

1

1

1

-I-

1

BH3-MJLT4S BH3WLT4S

-1

01

U

U

-1-

-

1

1

2

1

111

1

-141

U

1

-

1

-

1

2

Stage plate glass

Stage

gnp

for nght-hand s~de

Rubber damper to absorb
shock

BH3-SICl28-R 1 1
BH3-SICIO-R

]

BH3-SIC1410-R

BH3-SPG 106 1
BH3-SPG128
BH3-SPG 10
BH3-SPG 141
BH3-SGR-R
BH3-DMP

I

-I_

-

-

1

I

_

0

-

1

-

-

-

1

-

-

-

-

1

ljl

-1-

-

1

-

-

-

-

-

1

-

-

-

-

1

-

- -

-

I

!

-

l1-

-

1

1
111

1
1

I

'The illustration below shows the

BH3-MJLT4-883U

combination.

Eyepiece

-

Observation
tube

,Vertical illuminator

Objective magnification

Vertical illuminator
connecting cord

button

Levelling

Lamp housing for

transmitted light

screw1 Stage clutch knob

evolving

nosepiece control box socket

Revolving nosepiece remote control box

(optional)

Universal Vertical llluminator

ktandard Accessories for universal Illuminator

Filter insertion slot

Field iris diaphragm
centering knob

1

.I.

_

-. .

Half-mirror unit

indicator dial

Analyzer insertion

UV-protective shield1

breath shield mounting pins

Half-mirror unit

selector knob

I

Accessories for Universal Venical llluminator

slot

Huxlllary Ins

d

*\\-'.-

/

'-;ertion knob

'I

ND filterhint plate \ \PoIarizer insert~on

insertion slot

I

I

\

Eimlrl

I

,V,"

iric

rlianhranm

I,,"

".",...."J...

.

lever

.---.

.

.

.

slot

Brightfield half-mirror unit

BH2-UBF

Universal analyzer slider Universal tint plate slider Universal polarizer slider

BH2-UAM BH2-UTP530 BH2-UP0

Nomarski prism attachment Nomarski prism mount Brightfield objective adapter

U-NIC NRE-MAD

Darkfield half-mirror unit

BH2-UDF

3

3-1

Assembly

Outline

The dlagram below
dlagram covers all modules, select the steps pertinent to your modules and assemble
fluorescence

+r

Before assembly, wipe off all glass and connecting surfaces with a soh cloth or gauze in order to remove any
dust or dirt and carefully follow the assembly procedure illustrated below.

"

Modules necessary for the BH3-MJLT version

"

Optional modules

illustrates

comblnatlon, refer to the Instruction manual for the reflected llght fluorescence attachment @HZ-URFL)

(available

the assembly procedure and the numbers lndlcate the order of assembly. Although the

accordingly.

for some versions)

Motorized revolving

nosepiece

When assembling the

Wafer holder ring

Wafer holder plate

Mask holder

-

I

,

A

chip

-1

!-1

II*

Stage plate Stage plate glass

0

7

'9

Stage grip

I

I

.

DarK~eld objective

adapter

..

0

I

t

Nomarski prism
attachment"

Brightfieldldarkfield
(Neo) objective

Q

El

II

Polarizer for transmitted
light illumination

GI

3

3

t

2

;

:I

i

1

i

i

f

i

Eyepiece

Brightfield Darkfield

half-mirror half-mirror

I

:

I

t.

Slider with

360"

rotatable

Roof cover

Filter for universal

verrical illumintor

I

1

I

!

i

1

-,,

Breath shield

Breath shield mounting bracket

Tint plate sliderO

Halogen lamp housing for universal vertical illuminator

Polarizer

0

slider

Collector lens

Microscope stand

Halogen bulb

12V

50W

(JC

12V

Power cord

halogen bulb

50W

HAL-L)

Halogen lamp housing

for transmitted light

m

Stages and Related Accessories

Wafer holder ring

For

4", 3" wafers (BH2-WHR43)

For

Y,

4"

wafers (BH2-WHR54)

For 6",

5"

wafers (BH2-WHR65)

I

Silica chip

Q

(BH3-HCH)

Water holder plate

(BH3-WHPR86)

I

Wafer holder plate

(BH3-WHP6)

I

5" x 5"

(BH3-MH5)

4" x 4"

(BH3-MH4)

6" x 6"

(BH3-SP6)

mask holder

mask holder

stage plate

7" x 7" mask holder

(BH3-MH7)

I

6"

x

6"

mask holder

(BH3-MH6)

8"

x

8" stage plate

(BH3-SP8)

Stage plate glass

(BH3-SPG8)

Stage plate glass

(BH3-SPG6)

6"

unit

BH3-SIC8

-l

for

6"

x

6*

stage plate

(BH3-SP106) (BH3-SP128)

Stage plate glass

(BH3-SPG106) (BH3-SPG128)

12" x 8" stage plate

12" x 8" stage

IIBH3-SIC128)I

10"

x

10"

stage

14* x 10" stage

34

Preparation for

Assembly

Fig.

The stage will begin to slide at an inclination of

clutch disengaged, because the free-floating gearless rail system is ex-

it

tremely smooth. Therefore,
before installation of the microscope.

is very important to properly level

i

1.

Gently tip the microscope cover, as shown In Fig.

2.

Determrne
microscope base

selector

1

After placing the microscope stand, individually turn both levelling screws

@at the bottom of the microscope base until they make contact with the
table surface. Then turn the screws a half or one full turn further to stabilize
the microscope.

rf

the l~ne voltage selector switch @ at the bottom of the

IS

set to your local voltage. If not, set the line voltage

swltch to the correct posltion using a

lo

(71400

--

-

-I-

1.

screwdriver

mm) with the

the

table

i~i~~<

..

.-

or slm~lar tool

Fig.

2

Detailed

Back

Assembly

Procedure

1.

2.

3.

4.

5.

6.

7.

8.

Remove the transport locking plates @ on the rear of the stage using the

Allena wrench provided.
Turn the coarse focus adjustment knob @ to bring the focus unit

down to its lowest position.

Place the stage gently onto the focus unit and align the mounting holes

@

on the stage with the screw holes @ on the focus unit.

Attach the stage to the focus unit by lightly tightening the four hex screws

with the
Push in the stage as far as it will go, making sure that the stage does not

make contact with the pillar.
Securely clamp the stage with the Allen" wrench.
Remove the transport locking plates @ on the front of the stage with the

Alleng wrench.
Attach the caps to the four screw holes.

*

*

*

Allene wrench provided.

Lower the focus unit as much as possible before removing the
stage.

The stage can also be rotated a full

Keep the transport locking plates removed from the front and back
of the stage in an appropriate place and use for reclamping the
stage when it is removed.

180"

for mounting.

@

Fig.

Fig.

3

*

For

BH3-MHL

Interference Contrast Attachment, perform the procedures discussed

in paragraphs

*

When mounting the brightfield objective to the darkfield revolving

nosepiece, perform the procedure in paragraph

1.

Paste the labels provided near the objective apertures.

2.

Screw the objectives into the apertures marked
ascending or descending order of magnification.

4

1.

Loosen the clamping screw @ of the Nomarski prism attachment, shown
in Fig.

6,

with the Allena wrench and remove the Nomarski prism mount

@,

shown in Fig.

2.

Screw the Nomarski prism mount @ securely into the nosepiece with the
mounting tool provided.

combinations provided with the Nomarski Differential

Q

and

.

.

'A'

through

5,

from the Nomarski prism attachment.

'E",

in either

Fig.

5

Fig.

Fig.

6

7

Similar to the objective mounting procedure discussed in Section

the

Nomarski prism attachments onto the circular dovetails of the Nomarski
prism mounts, and temporarily clamp the prism clamping screw @ with
the Allen8 wrench.

Ir

Mount each Nomarski prism attachment in such a manner that the
straight line passing through the centers of the nosepiece and the
optical axis of each objective (Fig.

screws only after all attachments are mounted correctly.

Ir

When using brightfield objectives, screw the brightfield objective
adapters (NRE-MAD) into the

mounting the objectives.

1.

Gently insert the motorized revolving nosepiece @ along the slide dovetail

@

as shown in Fig.

Ir

DO not force the nosepiece when tilted.

Ir

A slight resistance may be felt at the bottom of the slide dovetail
due to the engagement with the connector. Insert the nosepiece as
far as it will go.

2.

To clamp the dovetail slide, use the 3 mm Allena wrench provided. to
lightly tighten the hex screw

7,

pressing the nosepiece against one side of the slide dovetail.

3.

Clamp the revolving nosepiece by tightening the four hex screws @ shown
in Figs.
the Allena wrench.

7,

8

(short screws for the top and long screws for the front) using

7.

@

6).

Tighten the prism clamping

Nomarski prism attachments before

on the side of the arm as shown in

2,

mount

Fig.

?

I

i

Fig.

I

8

Align the breath shield mounting bracket with two pins on the front of the
vertical illuminator and insert it.
Securely clamp the screws with the Allens wrench.

Fig.

9

I

Fig. 10

Mounting.4he Universal Vertical Illuminator

Lamp

\Housing

1.

Fully loosen the vertical illuminator clamping screw @ as shown in Fig.

10

with the Allenm wrench, and attach the vertical illuminator to the top
of the motorized revolving nosepiece. Clamp the unit lightly so that it is
parallel to the arm. (Fig.

+

Fully tighten the clamping screw @ only after having attached the

lamp housing. (Fig. 10)

1

1)

--

and

-

?Figs.

-

9,

-

30)

..

5i

!!

Fig. 11

Fig. 12

2.

Mount the collector lens and lamp housing.

[

For the 50W Halogen Lamp Housing (BH2-ULSH)

Screw the BH2-UCLW collector lens @ provided with the BHZ-ULSH
lamp housing into the universal vertical illuminator, insert the lamp housing

@as far as it will go, and then completely tighten the lamp housing clamping
screw

1

Screw the BH2-HCL collector lens @ provided with the BH2-HLSH lamp

housing into the universal vertical illuminator, insert the lamp housing
as far as it will go, and then completely tighten the lamp housing clamping
screw

I

For funher details, refer to the instruction manual for the BH2-URFL reflected
light fluorescence attachment.

*

*

3.

After mounting of the lamp housing is completed, adjust the universal
vertical illuminator so that it is parallel with the arm and completely tighten
the illuminator clamping screw

@.

For the lOOW Halosen Lamo Housina (BH2-HLSH)

8.

For Fluorescence Lam~

The fluorescence lamp housing cannot be mounted to the 10"

Use the BH3-UET8 extension tube especially designed for the BH3-

MJL.

HOUS~~~(BHP-LSRF)

@.

(Figs.

-

9,

10)

(Fig. 11)

(Fia. 12)

--

]

1

@

1

arm.

Fig. 13

Fig. 14

~Mou~t1n~g;MakIalpppn

1.

!

*

The BH3-MJL is available with two types of light sources for the

universal vertical illuminator

(

Mounting the Bulb (Fig. 14)

1.

Pressing the spring levers @ in the direction of the ARROWS, insert the
pins of the halogen bulb
the socket holes

2.

Release the spring levers to hold the bulb in position.

*

Never put the lOOW bulb in the 50W lamp housing.

*

DO not touch the bulb with bare fingers. If any fingerprints or din
are on the bulb, wipe clean.

*

Before replacing the bulb during microscopy, unplug the power

cord or turn

I

Mounting the Socket and Cord Assembly

1.

Loosen the bulb socket clamping knob

2.

Aligning the bulb socket clamping knob with the positioning groove @ of
the lamp housing,
and tighten the bulb socket clamping knob.

-

.

@.

off

the power and wait until the bulb cools.

insen the socket and cord assembly into the lamp housing,

Bulb,and

-.A----.&

(JC

12V

Bull@ike$for

-A

:

-

50W or lOOW halogen.

50W HAL-L or

12V

@.

-

-

100W

the

VerticalI\

=

HAL-L)

(Fig.

into

15)

J

1

]

Fig. 15

16

Fig.

*

Use the BHS-LSH5O for

1

Mounting the Bulb

1.

Loosen the lamp housing clamping knob @ of the lamp housing for the
transmitted light illuminator and remove the bulb socket.

2.

Pressing the spring levers

pins of the halogen bulb

8"

arm and the BH3-LSH50-10 for

@I

in the direction of the ARROWS, insert the

(JC

12V

50W

HAL-L)

into the socket holes

10"

arm.

1

Q.

Fig. 17

I

Mounting the Bulb Socket and Lamp Housing

1. Fully insert the bulb socket into the lamp housing for the transmitted light
illuminator and tighten the lamp housing knob. (See Fig.

2.

Insert the lamp housing into the lamp housing mounting port on the
micrsocope stand as far as it will go.

*

When the

10) into the mounting poiat the bottom

1. Fully loosen the observation tube clamping knob @ of the universal vertical

illuminator.

2.

Mount the observation tube on the universal vertical illuminator and tighten
the observation tube clamping knob securely, after aligning the eyepiece
sleeves to

10"

arm is in use, insert the lamp housing (BH3-LSH50-

bf

mint forward.

16

the arm.

on page

17.)

4

EM

Fig.

18

Fig. 19

lnsen the eyepieces into the eyepiece sleeves

--

When Using the Superwide Field Observation Tube

(BHP-SWTR) (Fig.

1. Aligning the positioning pin of the field-of-view eyepiece
with the positioning groove @ at the upper edge of the right eyepiece
sleeve, insert the eyepiece.

2.

Aligning the positioning pin of the eyepiece (SWHKlOX) with the position-

ing groove

eyepiece.

[

When Using the Trinocular Tube

1.

Aligning the positioning pin of the field-of-view eyepiece
with the positioning groove @ of the right eyepiece sleeve, insert the
eyepiece.

2.

Insert the eyepiece (WHKlOX) into the left eyepiece sleeve.

@

at the lower edge of the left eyepiece sleeve, insert the

(BH~-T~O/BH~-TTR)

@.

(35

(~=9(

(35

WHKlOX)

SWHlOX)

18)

Fig.

20

Mo-unting

1.

Remove the right and left half-mirror unit compartment cover plates
(Fig.

19)

fingertips.

.k

Half-mirror unit compartment cover plates are attached with a
magnet. Set them aside carefully.

2.

Remove the auxiliary mounting knob @ provided with the universal illuminator
shown in Fig. 21 and attach it to the brightfield half-mirror unit
Looking at Fig.
brightfield
is heard.

+

A

click will operate as soon as the half mirror unit is fully inserted

and its side panel is flush with the body of the vertical illuminator.

.k

Half-mirror units can be inserted from either the right or left side
of the universal vertical illuminator.

.k

For brightfieldidarkfield and universal versions, mount the darkfield

half-mirror unit

3.

Remove the auxiliary mounting knob from the half-mirror unit and reattach

to the universal vertical illuminator.

4.

Reattach the half-mirror unit compartment cover plates.

5.

Set the half-mirror unit indicator dial
half-mirror

.k

When the brightfield half-mirror unit is mounted on the right-hand
side of the illuminator, set the left-hand side half-mirror unit indicator

dial to the

the

Half-mircxunie

from the universal vertical illuminator by grasping them with your

22,

hold the auxiliary knob @ and carefully insert the

half:mirror unit into the half-mirror unit compartment until a click

(BH2-UDF) following procedure

unit, to the 'B.F.' or 'D.F.' position.

*B.F.* position.

-

-

(Figs.

20,

(BH2-UBF).

2.

@,

located opposite the mounted

21,2;!?

@

Fig.

Fig.

21

22

Insert the connecting cord plug @ of the vertical illuminator socket and
cord assembly

*

When connecting, align the connector pin of the connecting cord
with the groove on the microscope base.

Into the receptacle at the rear of the microscope base.

Fig.

23

L

=-TP

Typical example

of

3-pin grounding

Fig. 24

Fig. 25

plug

Connecting

Connect the primary cord with a 3-pin plug (it will fit into a ground type
power outlet, and no need to connect it to any other grounding device)

to

a

receptacle (wall mains outlet). (Fig.

*

If a 2-pin grounding plug is used, ground the microscope to a properly
grounded device (except a gas pipe). If necessary, use an extension
cord.

Ir

This microscope incorporates a noise filter in the electric circuit

built-in the microscope base, bleeding a very low voltage current
in order to reduce effect of any external noises. Therefore, if the
conductive part of the stand is touched without grounding, an electric
shock may sometimes be felt depending upon the humidity
conditions of the hands and foot wears.

1.

Turn

ON

2.

If

the LED light of an objective magnification button is blinking, rotate the

revolving nosepiece manually until it clicks.

3. If the lit LED of the objective magnification button and the engaged objec­tive match. the Setting is complete.

4.

If not, depress the lit button for 2 seconds. The lit button will change from

"A'

through to 'E". Release the button when it matches the engaged objec-

tive.

5.

Attach the labels showing the magnification powers of the mounted objectives
to the convex sections
buttons.

_.

_->__

the

Power

_

the power switch.

@

Cord

-.__

-

_

_

_

_

.___

__

-

-__

(Fig.34)-

24)

below the respective objective magnification

m

D

$

3

m

2

Fig.

Fig.

26

27

*

Lower the stage as much as possible to prevent it from hitting the

objective.

I

Mounting the Wafer Holder Plate

1.

Insert the edge of the wafer holder plate @ into the stage.

2.

Pressing from the top, make sure that the wafer holder plate is firmly in
position.

3. Tighten the holder clamping screw

1.5

with the

4.

Move the stage towards the right as far as it will go.

5.

Mount the wafer holder ring @ onto the mounting pin @ of the wafer
holder plate.

mm AllenE wrench.

Q

on the left-hand side of the stage

(Figs. 25,

26)

1

Fig.

28

I

Mounting the Mask Holder (for BH3-MJLT)

1.

Gently place the mask holder @ on the stage and check that it is firmly
in position.

2.

Tighten the holder clamping screw @ at the left-hand side of the stage
with the

1.5

mm AllenB wrench.

(Fig.

28)

]

Fig. 29

Fig. 30

-

(Fia. 29)

---

(Fig. 30)

@

---.

I

Mountina Other Holder Plates

Insert the holder into the stage and tighten the holder clamping screw

at the left-hand side of the stage with the

*

Do not tighten the screw with excessive force when mounting the
stage plate glass so as not to break the glass.

MquntJn-g,

I

For Reflected Light DIC Observation

With the inscription 'NIC' facing the operator, insert the universal polarizer

@

slider

*

Engage the polarizer slider into the light path. (First click)

I

For Simple Polarizing Observation

With the inscription
slider into the polarizer insertion slot of the vertical illuminator.

*

Since the polarizer slider has two click stops, push it all the way
into the light path. (Second click)

the

universal

into the polarizer insertion slot of the vertical illuminator.

'PO'

Polarizer

facing the operator, insert the universal polarizer

1.5

mm Allen3 wrench.

Slider

'

1

1

Fig.

31

1.

Remove the dust slider from the vertical illuminator.

2.

With the inscription facing up, push the universal analyzer slider @ into
the insertion slot until it stops. The analyzer slider can be engaged or
disengaged by pushing or pulling until a click is heard.

*

To prevent dust from entering the vertical illuminator, be sure to
insert the dust slider when the universal analyzer slider is not in
use.

*

When performing transmitted light simple polarized light observation,

use the analyzer slider with

360° rotatable analyzer (BH3-UAN360).

L

Mounting.

ND

I.

i

I

Fig. 32

Filter Slider

Insert the universal tlnt plate slider (or the ND fllter sl~der) lnto the ND
filterltnt plate Insertion slot on the vertical ~llum~nator. (See the

*

Engage the empty aperture of the tint plate slider into the light
path.

*

with the inscription facing the operator, insert the ND filter slider
so that the ND silter

;

M~~uMingrth~ Sglarizer
IllUmipatJop+

The polarizer for transmitted light illumination should be mounted only
when performing a transmitted light simple polarized light observation.
Mount the polarizer
positioning dots.

*

Use the 360" rotatable analyzer (BH3-UAN360).

the

Universal Tint Plate Slider

is

engaged into the light path.

fon

T~q~srnit-d, Ught

1

@

-.

onto the condenser mounting port by aligning the

or

-

-

--

-+

(Fig,

32)

ARROW.)

L

'(Fig.

-

@)

Fig. 33

Fig. 34

5

Mounting>%

.

. -.

1.

Loosen the two clamping screws on the top surface of the breath shield
mounting bracket (Fig.
screws. Tighten the screws securely.

Breath

34)

-.

Shield

-

-

and insert the breath shield underneath the

.

.

-

.-

(Fig.

344

Fig.

Fig.

35

36

After adjusting the aperture iris diaphragm and field iris diaphragm according
to image requirements and inserting the necessary filter, place the

roof cover @ on the arm.

+

Depending on the observation tube in use, the cover may be difficult

a

to mount. If that is the case, mount it in

of

the

direction

+

The pinhole unit

DMPLIOOXPC.

With the inscription facing the operator, insert the pinhole unit into the

filter insertion slot of the universal vertical illuminator.

ARROW.

is

used with

100X

titled fashion in the

objectives such as

UMA

LW-

Fig.

Fig.

37

38

box

Plug the nosepiece remote control
on the rear left-hand side of the microscope base.

Mount the stage grip below the projection at the rear of the stage and
tighten the two screws with the

cable into the multi-pin socket

2.5

mrn Allena wrench provided.

@

Two

vibration

damping pads

(marked

with

'F')

I

Fig. 39

Fig.

40

I

Mo~*g.th.

Ir

Do not use the vibration damping pads for

Ir

Vibration damping pads are used to prevent external vibrations
from being transmitted to the microscope stand. Use as necessary.

Place

the vibration damping pads at the bottom of the microscope base

after placing the microscope stand on the table as shown in the drawing.

:

Mo~nting!~the

-

---

1.

When mounting a brightfield objective to the motorized revolving nose-

(BH3-5NRE-M), first mount the objective adapter (NRE-MAD) in the

piece
threaded hole of the revolving nosepiece, and
into the adapter. (Fig.

2.

If one or more Nomarski prism attachments @ are mounted on the motor-

ized revolving nosepiece

focal distance when the objective adapter is in use, first mount the objec­tive adapter

41)

(Fig.

3.

If

the brightfieldidarkfield objective is to be used under the same condi-

tions, first mount the objective adapter

the brightfieldldarkfield objective. (Fig.

\?bWon

Oyective . Adapter

40)

(NRE-MSP2) @ and then mount the brightfield objective.

'?am~k!9-.P~d!_-

.-

(BH3-ENRE-M) and you want to obtain the same

.

.

insert the bright-field objective

(NRE-NESP2) 0 and then mount

41)

-

-

6"

stage (BH3-SIC6).

-

-

-

(Fip,

--

(Fig.

40,41!

391

]

1

;

Fig.

41

--T

Rqlaqi.ms_tofthe,

Since the focus unit and arm of your microscope are replaced only by the
manufacturer, contact your nearest Olympus office (see back page).

Fop~Inlt:~mjd

the@rm1=

'

11

.--a

Fig.

Fig.

42

43

In accordance with the half-mirror unit indicator dial

Q

mirror unit selector knob

*

Slide the half-mirror unit selector knob until it stops.

1.

Confirm that any one of the objective magnification buttons is lit.

*

If any button is blinking, the corresponding objective is not engaged.

a

Press

*

Do not rotate the revolving nosepiece manually, as memory setting
may change or a malfunction may result.

2. Press the button for the objective of choice to engage.

[

Alternative Objectives

Two objectives can be interchanged alternately.

button other than the blinking button.

to the desired position.

@,

slide the half-

I

1.

Select the first objective and press the corresponding objective magnification
button.

2.

Pressing the second objective's button completes the setting.

3.

By pressing the recall button

I

Emergency Termination of Nosepiece Revolution

If an objective magnification button is pressed by mistake, immediately
press the recall button. The revolving nosepiece will immediately return to
the original position.

1.

Turn the voltage adjustment knob @ on the right-hand side of the micrsocope
base towards the front ('low voltage' position) and turn on the power
switch

2.

To increase the light intensity, slide the voltage adjustment knob backward
('high voltage' position).

*

@

on the rear panel.

To adjust the light intensity without affecting the color temperature
during photomicrography, etc., use an
For further details, refer to paragraph

@,

only the two objectives can be alternated.

(Fig.

45)

ND

filter provided.

4

,

"Filter Insertion".

1

Fig.

44

Switching from Vertical Illuminator to Transmitted Light Illuminator

(Fins.

44, 45)

Fig.

45

Press the illuminator selector switch

vertical illuminator and the transmitted light illuminator.

EPI: Reflected light
DIA: Transmitted light

*.

When switching between the vertical and transmitted light il-

luminators during observation, adjust the transmitted light intensity

ND

with an

adjustment knob, in order to obtain equal brightness in reflected
light and transmitted light.

*

To use on the vertical illuminator and the transmitted light illuminator

simultaneously, a separate power supply unit
illuminator is needed.

I

Fuse Replacement

1.

Turn off the power switch and unplug the power cord from the AC outlet.

2.

Insert a Phillips screwdriver into the fuse holder @ at the rear of the
microscope base, turn counterclockwise and remove the fuse holder.

3.

Remove the fuse from the fuse holder and replace with a new fuse.

Applicable fuse:

filter and frosted filter without touching the voltage

@I

(Fig.

44)

to switch between the

(TH-3) for the vertical

D

2

z

Fig.

46

.

.

,,.

,,

I

Fo..lli.a:

The specimen can be brought into focus by raising and lowering the stage

using the coarse focus adjustment knobs
knobs

@I.

in the direction of the

approach each other).

The rotational tension of the coarse focus adjustment knobs is adjustable

by rotating the outer tension adjustment ring
increases the tension, while turning it counterclockwise decreases the
tension.

+

If loosened excessively, the stage may drop or defocus even after
the specimen is brought into focus with the fine focus adjustment
knob.
adjustment ring clockwise.

,.

,

..

.-;:

..

__

When the fine and coarse focus adjustment knobs are turned

ARROW,

If

that is the case, increase the tension by rotating the tension

the stage

.

.-

.

.-:.

_

a

will

rise (the objective and specimen

6).

.

,

.

,

^

__--I--.-

and fine focus adjustment

Rotating the ring clockwise

..

,:,

:.

.

(Fig.

461

.

L.

:

Fig.

47

Fig.

48

10

Use

of

Coarse

The coarse focus stopper is provided to prevent the objective from hitting

the specimen and to speed focusing. Locking the coarse focus stopper
lever

@

by turning it in the direction of the

focus adjustment knob's upper limit.

*

The fine focus adjustment knob's movement is not affected.

*

Even when replacing the specimen by lowering the stage once,
the specimen can be brought into an approximate focus by raising
the stage up to the upper limit specified by the coarse focus stopper.

Then, by making fine focus adjustment with the fine focus adjustment

knob, the specimen can quickly be brought into focus.

1.

Press the objective magnification button to engage the

2.

Looking through the eyepieces with both eyes, adjust the interpupillary
distance by sliding the knurled dovetail slides @ of the right and left
eyepiece sleeves until perfect binocular vision is obtained.

*

The knurled dovetail slides are provided with an interpupillary

distance scale

may wish to use the scale for rapid adjustment of your I.P.D.

Focus

@.

Stopper

ARROW

Once you know your interpupillary distance, you

will define the coarse

10X

(Fig.

objective.

rQ8)

Fig.

Fig.

49

50

1.

Looking through the ri'
into focus using the coarse and fine focus adjustment knobs.

2.

Looking through the left eyepiece with the left eye, rotate the diopter
adjustment ring

When a Field-of-view Eyepiece
Trinocular Tube

1. Looking through the field-of-view eyepiece with the right eye, rotate the

knurled collar

511 can be sharply distinguished as two separate lines. Then bring

(Fig.
the specimen into focus using the coarse and fine focus adjustment knobs.

2.

Looking through the left eyepiece with the left eye, rotate the diopter
adjustment ring

@

ght eyepiece with the right eye, bring the specimen

@

to bring the specimen into focus.

(I35)WHKlOX) is used

.

(BH2-TR3-

on the eyepiece (Fig.

@

(Fig. 50) to bring the specimen into focus.

--

--

O/BHZ-TTR) (Figs. 50, 51)

50)

until the double reticle lines

1

Fig.

51

Fig.

52

When Using the Superwide Field Trinocular Tube

1.

Looking through the right field-of-view eyepiece with the right eye, rotate

@

the knurled collar

can be sharply distinguished as two separate lines.

2.

Adjust the coarse and fine focus adjustment knobs so that both the reticle

lines and the specimen are sharply in focus.

3.

Looking through the left eyepiece, rotate the knurled collar @ on the
eyepiece to bring the specimen into focus.

on the eyepiece until the double reticle lines (Fig.

(BH2-SWTR)

(Fig.

52)

51)

Light

path

selector

knob

Indication

--

Light

path

Applica­tion

Fig.

53

I

!

IN

i

I

i

V

(white)

/

1.00%

binocular

1

tube

,

Obsewa-

/

tion of with focusing specimens

dark

specimens

Halfway OUT

1

C

-V
2%

to

binocular tube

80%

raphy

through

/

binocular

j

tub

(green)

to
to

C

100% to

photo

of

'

(red)

dark

Tilting the Obs-ervation Tube (for

The observation tube can be tilted to an angle for viewing in the most
comfortable position. Grasp the observation tube and raise or lower it to
the desired position.

Salectingithe

1.

Slide the light path selector knob @ on the right-hand side of the trinocular
tube to the desired position (Table

'

.

Light

Path

(for Trinoculgr Tube)

2d

BH2-lTRJlBl

.

-

- -

.

-..

:

,{Fig.

1)

until a click is heard.

,

--

-

only)

--

52,

(Fig.

Tabla

5311

--

.-I-

l)d

,

:

2

A

m

%

rn

n

z

0

z

Table

1

There are two positions to choose from:

Pushed in: For observation only (100% to binocular)
Pulled out: 20% to binocular tube, 80% to photo tube

Fig.

54

Reduced image

9el@ Iris Diaphragm Centratlon fqr Vertical Illuminatol:

--

.

(Figs.

54,

1.

Press the objective magnification button to engage the

the specimen on the stage, and bring it into focus.

2.

Turn the field iris diaphragm lever @ of the vertical illuminator clockwise

stopdown the field iris diaphragm

to

3.

Rotate the right and left field iris diaphragm centering knobs @ of the

vertical illuminator counterclockwise to bring the reduced diaphragm image

into the center of the field of view.

4.

Rotate the field iris diaphragm lever counterclockwise in order to widen
the opening of the field iris diaphragm until the polygonal image of the
diaphragm is barely touching the rim of the circle indicating the field of
view. If the image is off center, repeat the field iris diaphragm centering
operation.

5.

Continue widening the opening of the diaphragm until the reduced image
circumscribes the field of view.

to

a minimum.

10X

objective, place

-

66)

-

Fig.

Fig.

55

56

+permre

Since it is pre-aligned, aperture iris diaphragm centering adjustment is un­necessary.

1.

Engage the

2.

Remove one of the eyepieces from the eyepiece sleeve and look at the

objective pupil through the empty eyepiece sleeve.

3.

Turn the centering screws @ of the pinhole unit to bring the pinhole to
the center of the objective pupil.

This step adjusts the beam of light on the specimen to achieve the optimum

image contrast.

iris

Diaphragm Centration forvertcal llluminatpr

100X

or higher power objective.

Fig.

57

(

Reflected Light Brightfield Observation (Fig.

Depending

of the unrversal vertrcal lllumlnator clockw~se to reduce the
openlng unt~l the reduced Image c~rcumscr~bes the fteld of vlew and shuts
out unnecessary

use.)

(

Reflected Light Darkfield Observation

When performing
fully by turning the field iris diaphragm lever of the universal vertical illuminator
counterclockwise.

on the

objective

Ilght.

the

In use, turn the f~eld Iris d~aphragm lever

(This

settlng varies wlth the

darkfield observation, open the field irls diaphragm

objective

58)

@

diaphragm

power In

(

1'

a

t

H.

8

'

F

-F

.&

B

This step adjusts the numerical aperture of the vertical illuminator which
influences the resolution, contrast, and focal depth of the observed image.

* When observing under reflected light, a glare can occur due to an

effect of the condenser for transmitted light depending upon the

(Fig.

the

58)

specimen to be observed. Be sure to reduce the opening of

disphragm to its minimum by turning the aperture iris diaphragm
adjustment ring fully clockwise.

I

Reflected Light Brightfield Observation

]

Fig.

Fig.

58

59

Remove one of the eyepieces from the eyepiece sleeve and turn the
aperture iris diaphragm lever
diaphragm opening, while looking in the empty eyepiece sleeve. Turning
the aperture iris diaphragm lever clockwise reduces the opening of the
diaphragm.

Generally, reducing the diaphragm opening to

objective N.A. will provide an image of favorable quality.

(

Reflected Light Darkfield Observation

In case of reflected light darkfield observation, open the aperture iris dia­phragm fully by turning the aperture iris diaphragm lever counterclockwise.

*

Depending upon the specimen to be observed, slighly reduce the

diaphragm opening to obtain an excellent

minimal glare. Unlike in brightfield observation, the objective exit
pupil is not visible even after the eyepiece is removed. Adjust the
diaphragm while observing the image until an optimum image with

minimal glare is obtained.

1

Transmitted Light Brightfield Observation

Turn the aperture iris diaphragm adjustment ring @ of the condenser to
adjust the opening of the diaphragm. Rotating the aperture iris diaphragm

adjustment ring clockwise reduces the size of the opening.

@

on the vertical illuminator to adjust the

70

-

80%

darkfield image with

(Fig.

of the

59)

1

I

Fig.

60

Disengage the
path.

1.

Slide the half-mirror unit selector knob to bring the brightfield half-mirror
unit into the light path.

2.

Press the objective magnification button to engage the

3.

Place a highly reflective specimen such as a mirror on the stage, and bring
it into rough focus.

4.

Remove the right or left eyepiece, and adjust the bulb centering knob
and socket clamping knob @ so that the filament image visible through
the observation tube is located in the center of the objective pupil.

Rightneft: Turn the bulb centering knob
Upldown: Loosen the socket clamping knob @ and rotate in the

5. Engage the universal analyzer slider, polarizer slider, and filters.

analyzer slider, polarizer slider and filters from the light

10X

objective.

@.

direction of the ARROW. After adjusting, tighten the knob.

@

Fig. 61

Fig. 62

I

Reflected Light Observation (Fig. 61)

Generally, to change the color temperature of halogen light to daylight,
open the filter cover
20LBD3-W

20LBD3-W filter: Changes the color temperature of the illumination
201F550-W filter:

20ND6-W filter:

20ND25-W filter: Attenuates the light intensity.

*

If the filter is difficult to insert or remove, spread the sides of the

filter cover

*

When the filter is not in use, replace the filter cover to prevent dust
from entering.

I

Transmitted Light Observation

Mount the filter @ in the filter holder @ provided with the lamp housing

for the transmitted light illuminator and then insert the filter holder in the

filter insertion slot
tion.

45LBD3 filter: Changes the color temperature of the illumination
451F550 filter: Adds contrast to the observed image.

45ND6 filter: Attenuates the light intensity.
45ND25 filter: Attenuates the light intensity.

filter according to the purpose of observation.

@

of the universal vertical illuminator and insert the

to daylight.
Adds contrast to the observed image.
Attenuates the light intensity.

(light transmission:
(light transmission: 25%)

@I

and remove.

@.

Choose the filter according to the purpose of observa-

to daylight.

(light transmission: 6%)
(light transmission: 25%)

6%)

(Fig.

62)

1

(

Fig. 63

1.

Turn the clutch knob @ on the bottom right of the stage to the

64)

(Fig.

2.

The stage can be moved vertically (Y-axis direction) by rotating the upper

0,

section of the stage control knob
rotating the lower section of the knob.

*

Clutch knob should be set so that the mark faces the operator.

and horizontally (X-axis direction) by

'A'

position.

(

Use of Stage Clutch

(

Clutch knob (A)

I

(Figs.

64,

65)

1

@

@

Fig.

Fig.

64

65

0

0

When the clutch knob is set to
and Y axes, and fine travel of stage can be conducted by following the
previous procedure.

*

If the clutch
abrupt movement may cause the stage to slip.

I

Clutch knob

When the clutch knob is set to

X and Y axes, and stage travel is not possible with the stage control knob.
(The stage will not move when the stage control knob is turned.)
Since the stage travels lightly and smoothly,
distance by hand.
of the stage or the optional stage grip

[

Clutch knob (C)

When the clutch knob is set to

and disengaged
stage control knob. Since there is no X-axis travel, the specimen needing
repeated patterns can be observed conveniently by fixing the X-axis and
moving along the Y-axis.

[

Clutch knob

When the clutch knob is set to
and disengaged for the X-axis. This allows stage travel reversed to the

setting of the clutch knob.

*

Never move the stage manually with the clutch engaged, as this
may wear out or damage

is

engaged, turn the stage control knob slowly since

(8)

I

It

is convenient to grip the travel pins @ at the front

I

for

the Y-axis. This enables X-axis travel only with the

(Dl

I

'A",

the clutch is engaged for both the X

'B',

the clutch is disengaged for both the

it

can be moved over a large

@

when moving the stage.

'C',

the clutch is engaged for the X-axis

'D',

the clutch is engaged for the Y-axis

the

stage.

'C'

09

rn

n

5

0

Fig.

66

I

Tilt Adjustment of Holders and Plates

When placing a holder (BH3-WHPR86, etc.) or plate (BH3-SPG8, etc.) on

the stage, parallel adjustments of the stage and the top surfaces of the
holder

or

plate are not necessary since the assembly has been preset.

However, to make fine adjustments, apply a drop of alcohol to the screws

@,

unlock the screws and adjust them vertically using a slotted screw-

driver from the underside of the stage.

*

The stage can support up to 2 kgf

(4.4

Ib) including holders.

(Fig.

66)

1

The

BH3-MJLTJMJLA

simple polarizing, and fluorescence observation.

*

For reflected light fluorescence observation, refer to the instruction manual for the reflected light fluorescence
attachment

5-1

Reflected Light Brightfield/DarMield Observation

(

Preparation for Observation

allows a quick change between the brightfieldidarkfield. Nomarski differential interference contrast,

(BH2-URFL).

Fig.

67

~a~f-mirror

Confirm that the brightfield half-mirror unit and darkfield half-mirror unit
are inserted into the half-mirror unit compartment and slide the half-mirror

unit selector knob
path.

Engage the ND filter
minimizes changes in light intensity (glare) when changing the observation
method from darkf ield to brightfield.

If light intensity is insufficient during brightfield observation, or a reduced
exposure time is required for photomicrography, engage the empty aper­ture of the

UniKSeMng

@

to engage the desired half-mirror unit into the light

@

of the ND filter slider into the light path. This

NO filter slider.

-

,

,

I

.

-

(~ig.

67)!

68

Fig.

1

Observation Procedures

Engage the desired half-mirror unit with the half-mirror unit selector knob, according to the purpose of observation. p.
Disengage the universal analyzer slider, polarirer slider, tint plate slider and Nomarski prism attachment.

Press the illuminator selector switch to *EPIu and switch

1 4 1

1

Place the specimen to be observed on the stage.

Engage the

(

1 6 1 1

Adjust the aperture and field iris diaphragms.

-1

.

Check that the bulb is centered correctly.

lnsen the desired filter into the universal vertical illuminator.

10X

objective and focus.

-

.

ON

the miscroscope main switch, turning

ON

the halogen

-.

1 9 1

I

Engage the desired objective and focus again.

-1

-,

111(1

--

I

Adjust the light intensity with the voltage adjustment knob.
Turn the aperture iris diaphragm adjustment ring fully clockwise to reduce the opening of the diaphragm.

Brightfield observation

Darkfield observation

Adjust the field and aperture iris diaphragms.
Open the field and aperture iris diaphragm. pp.

pp.

p.

P.

p.

P

p.

29,
29,

p.

P.
p.

p.

p.

33

34

26

30

27

27

30

31

26
25

30

30
30

1

1

1

I

1

,

1

I

:

&'

5-2

Reflected Light Nomarski Differential Interference Contrast Observation

(

preparation for Observation

1

1

Fig.

69

Half-mirror

Slide the half-mirror unit selector knob to the
brightfield half-mirror unit into the light path.

Confirm that the universal analyzer slider
correctly pushed all the way into the universal vertical illuminator.

*

Make sure that the "NIC" inscription of the universal polarizer is

facing the operator.

*

When using the BH3-UAN360 analyzer, position the polarizer with

its

"NIC inscription facing the operator and turn the analyzer rotation

@

dial

Unit

Setting

'B.F.'

position to engage the

@

and polarizer slider

so that the dot is also facing the operator.

are

interference

color
Black

Magenta

Fig. 70

71

Fig.

Observation features

,

Darkfield-like observation
The highest gray sensitivity

observation

I

Even minute optical differences
(refraction, thickness, height,
etc.) can be observed as a color
change.

@

Move the prism control lever
'IN' position to bring the prism into the light path.

Turn the polarizer rotation dial @ of the universal polarizer slider until the
desired background color is obtained for maximum contrast of the specimen

under observation.

*

To obtain a magneta background, put the universal tint plate slider

into the ND
into light path.

*

The interference color can be changed continuously from black to

(0 - 700 nm)

blue

1.

Move the prism control knob to the

from the light path.

2.

Disengage the universal analyzer slider, polarizer slider and tint plate slider.

filterhint plate insertion slot and push it all the way

of the Nomarski prism attachment to the

'OUT

position to disengage the prism

Table

2

(

Observation Procedures

-.

Confirm that the half-mirror unit selector knob is at the

'B.F."

position.

-

Engage the universal analyzer slider, polarizer slider, and Nomarski prism attachment.

(

Press the illuminator selector switch to "EPI', and switch ON the microscope main switch, turning ON the halogen

I

bulb.

(Place the specimen to be observed on the stage.

-

15

1

Engage the

-

-

Perform diopter and interpu~illary distance adjustments.

(6

Check that the bulb is centered correctly.

-,

)8)

1

Insert the desired filter into the universal vertical illuminator.

-

191)

Engage the desired objective and focus again.

I

Adjust the light intensity with the voltage adjustment knob.

111

11

Adiust the aperture and field iris diaphragms.

---

Adjust interference color and contrast

[plate

53

Reflected

slider.

10X

objective and focus.

-

-

Light

Simple Observation in Polarized

by

turning the polarizer rotation dial and inserting or removing the tint

Light

PP.

341

p.

34

p.

p.

26

30

p.

27

p.
p.

27
30

p.

31

p.

26

p.

25

p.

29,30

p.

34

1

1

1

1

I

1

1

,

1

1

I

I

Preparation for Observation

Fig.

72

Ha!f:mitro;r.

Sl~de the half-mirror unlt selector knob to the
br~ghtf~eld half-m~rror unit Into the l~ght path.

8

,

Cl!e~king-@g Analyzer aa,dcPolarher

Confirm that the universal analyzer slider @ and polarizer slider @ are

correctly pushed all the way into the universal vertical illuminator.

*

Make sure that the "PO" inscription of the universal polarizer is
facing the operator.

*

When the
slider so that its "PO" inscription is facing the operator and turn the
analyzer rotation dial so that the dot is also facing the operator
(see Fig.

Engeging,

.~mchmed:

L

-

When the Nomarski prism attachment is mounted, swing the prism control

lever

@

of the Nomarski prism attachment to the 'OUT position to disengage

the prism from the light path.

unit.

set tie^

BH3-UAN360

70

on page

34).

a@$r[)Isengsgin@

-.-

--

-

.

.

--

-

--

-

-

-

-

-

- - -

analyzer is

the

---,

.

-

--

-

- - - - - - -

'B.F.'

posit~on to engage the

,

-

.

-

.

in

use, position the polarizer

NqmamllE~~Prism

I---

-.

(n&

.

IEal72)

---

-

-l

:I

,

-I

$$!;

Fig.

73

Setting

Turn the polarizer rotation dial @ of the universal polarizer slider so that

the dot faces to the side in order to obtain a crossed filter position.

Fig.

74

I

Observation Procedures

I

Confirm that the half-mirror unit selector knob is at the
Engage the universal analyzer slider, polarirer slider, and Nomerski prism attachment.

Disengage the Nomarski prism attachment.

Press the illuminator selector switch to "€PIe, and switch ON the microscope main switch, turning ON the halogen
bulb.

Place the specimen to be observed on the stage.

)

Engage the

10X

objective and focus.

thecrossed

'B.F.'

position.

Filter

Position

_

_

-_

-

-

(Fig,

73

p.

34

p.

34

1

p.

34

p.

26

p.

30

p.

27

Perform diopter and interpupillary distance adjustments.

Check that the bulb is centered correctly.

Insert the desired filter into the universal illuminator.

110

1

I

Turn the polarizer rotation dial until a crossed filter position is obtained.
Engage the desired objective and focus.

Adjust the light intensity with the voltage adjustment knob.
Adjust the aperture and field iris diaphragms. pp.

5-4

Transmitted Light Observation

Preparation for Observation

Select transmitted light by pressing the illuminator selector switch @ to

'DIA' position.

the

29,

p.
p.

p.

P.

p.

p.

27
30
31

36
26

25
30

1

Fig.

75

If

a

half-mirror unit is mounted, slide the half-mirror unit selector knob to

the

'B.F."

position to engage the brightfield half-mirror unit into the light

path.

*

If no half-mirror unit is mounted, observation is possible without

moving the half-mirror unit selector knob.

I

Observation Procedures

Slide the brightfield half-mirror unit into the light path (or confirm that no half-mirror unit is inserted into the
half-mirror unit compartment) p.

El

Press the illuminator selector switch to 'DIA", and switch

transmitted light halogen bulb.

El

Disengage the universal analyzer slider end Nomaraki prism attachment.

1

-

Place the specimen

1

Engage the
Perform diopter and interpupillary distance adjustments.

Insert the desired filter into the filter insertion slot on the transmitted light lamp housing.
Engage the desired objective and focus again.

to

be observed on the stage.

10X

objective and focus.

ON

the microscope main switch, turning

'

ON

the

-

Adjust the light intensity with the voltage adjustment knob.

~djun the aperture iris diaphragm.

1

5-5

Transmitted

Light

Simple

Observation in

-

--

Polarized

Light

p.

p.
p.

p.

p.
p.

p.
p.
p.

34

26

34

1

33

27

27

31

26

1

25

30

I

Preparation for Observation

Fig.

76

Selecting

.

-

.

.

- + -

Select transmitted light by pressing the illuminator selector switch @ to
the

'DIN position.

If the brightfield half-mirror unit is mounted, slide the half-mirror unit selector
knob to the
light path.

.k

If no half-mirror unit is mounted, observation
moving the half-mirror unit selector knob.

Ir

Use the BH3-UAN360 analyzer and the BH3-POL polarizer.

1.

Insert the polarizer into the condenser frame so that their positioning dots

@

are aligned with each other.

2.

Turn the analyzer rotation dial until a crossed filter position is obtained
with the dot facing the operator (see Fig.

the

-

-

lllumina~n-Method-

'B.F.'

position to engage the brightfield half-mirror unit into the

70

on page

.

-

---

is

possible without

34).

(Fig.

1

;!I61

Fig.

77

L

I

Observation Procedures

Slide the brightfield half-mirror unit into the light path (or confirm that no half-mirror unit is mounted in the

half-mirror unit compartment) p. 34
Press the illuminator selector switch to

transmitted light halogen bulb.
Engage the universal analyzer (BH3-UAN360) and the polarizer for transmitted light. p. 34
Disengage the Nomerski prism attachment.

1

Turn the analyzer rotation dial until a crossed filter position is attained.

1 6 1

1

Place the specimen to be observed on the staae.

I

Engage the

1 8 1

I

Perform diopter and inter~upillaw distance adiustment.

1OX

objective and focus.

'DIA', and switch On the microscope main switch, turning ON the

--

p.

26

p.

35

p. 36

P.

30

p.

27

P.

27

1

1

1

(

Insert the desired filter into the filter insertion slot on the transmitted light lamp housing.

110

(1

Engage the desired objective and focus again.

I

Adjust the aperture iris diaphragm.

p. 31

P.

26

p.

30

I

Fig.

78

With respect to handling photographic equipment, also refer to the instruc­tion manual for the camera in use.

Use

NFK

photo eyepieces specially designed for photomicrography. Insert
the photo eyepiece
tube.

@

into the photo tube of the trinocular observation

Light

knob

Indication

Light

path

Applio­tion

Fig.

Pulled out halfway

I

,

C

.V

(green)

20%

into

binocular tube

80%

into photo tube

photo tube

/

(focusing through

,

the binocular tube)

I

79

Pulled out fully

/

C

(red)

100%

into

Wunting.

Place the photomicrographic equipment on the photo tube of the trinocular
tube. Align the dots on the trinocular tube and the photomicrographic
equipment and clamp the photomicrographic equipment with the clamping
screw

With the BH2-TR30 Trinocular Tube or BH2-SWTR3O
Superwide Field Trinocular Tube

Pull out the light path selector knob @ on the right-hand side of the
trinocular tube to the

the-Photqm_!c_rpgmphicCEqu1~mment

O.

'C.V

or

"C'

position.

(Fig.

79,

-

(Fig.

Table

7%)

3)

Table 3

Fig.

80

[

With the BH2-TTR Tilting Trinocular Tube (Fig. 80)

Pull out the light path selector knob @ on the right-hand side of the tilting
trinocular tube to the

20%

light is directed to the binocular tube and

'C'

position in order to direct light to the photo tube.

80%

to the photo tube.

1

?

7

C

B

i

j;

i

Fig.

81

Focusing

Use the binocular tube of the trinocular tube for focusing on the film plane.

1.

If the viewer of the photomicrographic equipment is not to be used,
remove the viewer and attach the dust cap.

1.

Confirm that the field-of-view eyepiece is inserted into the right eyepiece
sleeve.

2.

Each field-of-view eyepiece has a focusing front lens and a reticle with
four frames, with each frame indicating the area covered by the specific
photo eyepiece:
The image in the focusing front lens and the image on the film plane are
in focus at the same time.

*

Several types of field-of-view eyepieces are available according to
the camera back in use.
standard field-of-view eyepieces.

Field-of-view

)35)

WHKlOX (or

-

135)

SWHKlOX) are

(Figl

81)

Camera

use

in

35

camera

mm

back

Sheet film or Intermediate

3-114' x 4-114'

Polaroidg back

adapter

4' x 5'

for

film

WHKlOX

(Field
number

20)

Type MSPlan

Magnification

N.A. 0.13 0.30 0.46 0.80 0.95

W.D.

Focal length Imm)

Resolving power (p)

--

(mm)

5X 1 OX 20X 50X

21.10 9.00 3.00 0.47 0.27

35.9 18.0 9.0 3.6 1.8

1.12 0.73

1

Fox

Total Focal

rnagnificati0+$~ depth Ip)

Field of view (mm)

Trr,

I

I

1

MDPlan 1 NeoDPlan

0.95

1

0.90

0.42

1.3

0.4 0.2 0.13 0.13

1 000X 0.67 1500X 0.55 l500X 0.60

1.2

Type

Magnification

N.A.

W.D.

Focal length (mm)

Resolving power

WHKl

OX

(Field Focal depth
number

WHKI

20)

OX

(Field Focal depth
number

WHKlOX

(Field
number

20)

20)

Total magnification

Field

Focal

Resolving power (p)

Total magnification
Field of view (mm)

Focal length (mm)

Resolving power

Total magnification
Field of view (mm)

(mm)

of

view (mml

Type ULWDMSPlanlULWDNeoSPlan

Magnification

N.A.

W.D.

(mm)

length (mm)

Type

Magnification

N.A.

W.D.

(mm)

Focal depth

(p)

(p)

(p)

(p)

(y)

.

NeoSPlanlNeoSPlanNlC

5X

0.13J0.13 0.30J0.25 0.46/0.40 0.80/0.70 0.90/0.90

10.00 3.00 0.50 0.30

35.9 3.6 1.8

2.58f2.58 0.4210.48

---

50X 200X 500X 1 000X

70.1 5.1 1.3 0.73
4

20X

0.4

11

.OO

9.0 3.6 2.25 1.80 3.60 0.72

0.84 0.61 0.45 0.42 0.35 0.37

200X

6.09

1

LWDNeoSPlanApo LWDMSPLZOX-LCD LWDMSPLSOX-LCD LWDMSPL100X-LCD

250X 20X 50X 1 OOX

0.90 0.40 0.60 0.80

0.80 7.87 3.02

0.72 9.0

0.37 0.84 0.56 0.42

2500X 200X 500X 1 OOOX

0.50 6.09 1.93 0.87

0.08 1 0.4

1

OX 20X 50X 1 OOX

0.3710.37

,

1

50X 8OX 1 OOX 5OX

0.55 0.75 0.80 0.90

8.00 4.10 3.20

0.4 0.2

LWDMSPlanApo

1

250X

:

0.80

I

500X 800X

2.18 1.07

0.4 0.25

1 OOOX

0.87

0.2

500X

1.04 0.50

0.4 0.08

3.60

I

2500X

1.04

1

.80

0.2

Note: All the objectives are dry type. Resolving power is calculated with the aperture iris diaphragm fully opened.

Technical terms:

W.D.

(Working Distance): The distance between the specimen and the nearest point of the objective.

N.A.

(Numerical Aperture]: The numerical aperture represents a performance number which can be compared to the

Resolving power:

Focal depth:

Field number:

of

Field

view diameter

relative aperture (f-number) of a camera lens.
the resolving powers of all types of objectives. The larger the
power.

The ability of an objective to register small details. The resolving power of an objective is

by

measured
The distance between the upper and lower limits

optical system. The smaller the opening of the aperture diaphragm or the objective
larger the focal depth.

This number represents the diameter in millimeters of the image of the field diaphragm that
is formed by the lens in front of it.

The actual size of the field of view in millimeters.

its ability to separate two points (resolution).

N.A.

values can be used for directly comparing

N.A.,

the higher the resolving

of

sharpness at an image formed by

N.A.,

an

the

LW

If you are unfamiliar with any aspect of this model or feel that performance is less than 100%. check the Items on the
follow~ng table.

.,

1.

Optical

-

.

System

Ttgublb

.

.

'

,;

.

,

.

-

!&Ius~~

.. .

,.

...

.

.

.

.,

.

...

,

.

.

.

.

'-..:~tj&&$'<

.

.

. . .

,

'

i

_/

1'

:

.

,

a. The bulb is burning, but image 1 The field iris diaphram is not opened Open until the diaphra m image cir-

cannot be seen or is dark.

cumscribes the field

o? view. (p. 291

The aperture iris dia hragm is stopped Open fully. (p. 30)
down with the dark!eld half-mirror unit
engaged (for reflected light

brightfield/

The light path selector knob of the
trinocular tube is between settings.

'

Pull it out to

'C.V' or

'V'

position.

(p. 28)

Set it to match the local line voltage.

incorrectly.

I

The half-mirror unit is not correctly
inserted into light path.

Enga e the half-mirror unit properly

with

8e halfmirror unit selector knob.

(p. 20)

(p. 25)

b. Image is partially obscured or

correctly.

Center the bulb.

(p. 30)

evenly illuminated.

Insert the universal analyzer, polarizer
and tint plate slider as far as it will

90.

(PP.

21. 22)

Pull it out to 'C.V or 'V position.

(p. 28)

Engage the half-mirror unit properly

with the half-mirror unit selector knob.

(p.

25)

Clean.

c.

Stains ordust can be seen in the
field of

wew.

The universal analyzer, polarizer or tint
plate slider is not inserted far enough.

The light path selector knob of the
trinocular tube is between settings.

The half-mirror unit is not correctly
inserted into the light path.

Dirty objectives
Dirty half-mirror units

,

Insufficient contrast
Image details lack definition

f.

One side of the image is out of
focus.

Dirty specimen

I-

Dirty condenser top lens

.-

Dirty objective front lens
Dirty eyepieces
Dirty prism on the bottom of the ob-

servation tube
Dirty stage plate glass

is stopped

--

correctly.

Dirty condenser

j

lens
Designated

and/or objective front

IC (infinity corrected) ob-

jectives and eyepieces are not used.
The specimen is not positioned cor-

rectly.

The objective is not engaged correctly.

,

Open the aperture iris diaphragm as
required.

(P.

Click the nosepiece into position.

(p. 30)

Clean.

Use designated

IC objectives and eye-

pieces.
Reposition the specimen on the stage.

Click the nosepiece into position

30)

.~ .

:

.

.%&ble

-

,

:,

..

,,

.

'

-

,

&use.

-:

Normaski Differential Interference Contrast Observation

g.

No interference color

.

.

~-

. .

,

.

.

..

-

'

R~*.Y

Place the universal analyzer and
polarizer correctly.

----

(p.

21)

-

-

--

--

h.

Interference color is uneven

--

ferential

low. used.

2. Observation Tube

interpupillary distance

Incomplete binocular vision

The
adjusted correctly.

pp

Diopter adjustment

3.

Stage

The stage is not clamped

The image goes out of focus
cessively when the stage is
touched.

4.

Coarse and Fine Focus Adjustment Knobs

a. The coarse focus adjustment knob

ex-

--

Stage clutch is disengaged. Engage the clutch. (p. 32)

The tension adjustment ring is tighten-

movement is stiff. ed excessively.

--

b. Poor focus during observation due

--

'

to unexpected lowering of the

is locked at the upper limit.
The tension adjustment ring is too

loose.

stage.

c. The stage cannot be raised com-

pletely with the coarse focus

The coarse focus stopper is locked
at the lower limit.

adjustment knob.

--

-.

is not

-

.

IS

incomplete.

-

7

-.

-

focus adjustment stopper

.-

-.

--..

--

I

Set correctly interpupillaw distance.

1

!

Complete diopter adjustment.(~. 27)

(p. 27)

(p.

(p. 14)

-

,

Loosen appropriately. (p. 26)

Release the coarse focus stopper.

I

Tighten appropriately.

(p. 27)
(p. 26)

Release the coarse focus stopper.

(p.

181

27)

5.

Electrical System

a. The bulb flickers.

b.

Although an objective LED lights
up, the bulb does not come on.

c. Light intensity at maximum voltage

adjustment range is insufficient.

d. Bulb does not burn.

"'Itage

and

Bulb

Fuse

Consum~tion

Power

The bulb is approaching the end of
its life.

Loose electric connections.

The bulb is burned out.

Loose electric connections.

The line voltage selector switch is not

set to your local line voltage.
Fuse has blown.

1

5V

AC,

10011

20012201240V

For reflected light:
For transmitted light:

AC

12V. 100W halogen bulb JC12V1 OOWHAL-L

12V.

50W halogen bulb JC12V5OWHAL-L

12V, 100W halogen bulb JC12VSOWHAL-L

4A

150VA (max.)

-.

-

50160

Replace the bulb.

-

Connect cords and plugs correctly.

Replace the bulb. (p. 17)
Connect cords and plugs correctly.
Switch to your local line voltage.

Replace the fuse. (p.

Hz

(p. 17)

(pp. 19, 20)

(p.

13)

26)

-

OLYMPUS

OLYMPUS OPTICAL CO., LTD.

San-Ei Building,

22-2.

Nishi Shinjuku 1-chorne, Shinjuku-ku, Tokyo, Japan

OLYMPUS OPTICAL CO., (EUROPA)

Wendenstrasse 14-1

6,

Postfach 104908,2000 Hamburg

1,

OLYMPUS CORPORATION

4

Nevada Drive, Lake Success,

OLYMPUS OPTICAL CO.

2-8 Honduras Street,

London

N.Y.

11042-1179,

EClYOTX,

(U.K.)

United

U.S.A.

Kingdom

GMBH.

Germany

LTD.

The design of the product is under constant review

.

,

..

.

- - -

4---

-L-

-:-k*

:-

LOM-,~~

and

whilst every effort is

t~

+ha-

~nwifir~.