Olympus BH2 BHSP User manual

OLYMPUS POLARIZING MICROSCOPE

[

INSTRUCTION

MANUAL

I

MODEL

BHSP

This instruction manual has been written for the use of the Olympus Polarizing Microscope Model

BHSP.

It

is

recommended to read the manual carefully in order to familiar-

ize yourself fully with the use of the microscope, so that you can obtain optimum per-

formance from it.

VL.__n_NWW___---x--

IMPORTANT

Observe the following points carefully:

Operation

1. Always handle the microscope with the care it deserves, and avoid abrupt motions.

2.

Avoid exposure of the microscope to direct sunlight, high temperature* and humidity, dust and vibration.

If the microscope is used in an ambient temperature higher than 40°c (104"F), it

may cause a trouble to the microscope.

3.

Only use the tension adjustment ring for altering the tension of the coarse adjustment. Do not twist the two coarse adjustment knobs in the opposite directions simultane-

ously, which might cause damage.

4. Ascertain that the line voltage selector switch on the base plate is set to conform with the local mains voltage.

Maintenance

1. Lenses must always be kept clean.

Fine dust on lens surfaces should be blown or wiped off by means of an air blower or a clean brush. Carefully wipe'off oil or fingerprints deposited on the lens surfaces with gause moistened with a small amount of xylene, alcohol or ether.

2.

Do not use organic solutions to wipe the surfaces of various components. Plastic parts, especially, should be cleaned with a neutral detergent.

3.

Never disassemble the microscope for repair. Only authorized Olympus service per-

sonnel should make repairs.

4.

The microscope should

be

stored in its container immediately after use. If this is not

possible, it should be covered with'a vinyl dust cover.

STANDARD EQUIPMENT

-

Component

Microscope stand (with Allen wrench,

KB-4 filter, immersion oil, and vinyl

dust cover, one each)

Line cord UYCP

Observation

tubes

Quadruple revolving nosepiece BH-PRE Polarizing intermediate attachment

(with pin hole cap)

Centerable circular stage

(with stage plate, centering wrenches,

paired, and stage clips, paired)

Test plates

100-watt halogen lamp housing

(with collector lens and filter holder,

one each) Pre-centered halogen bulbs 12VlOOWHAL-L Abbe polarizing condenser BH2-POC

Objectives

Eyepieces

Photo eyepiece NF K3.3X

Binocular tube BH2-B130

Trinocular tube

Quarter wave plate

(R

=

-

147.3my)

Sensitive tint plate

(R

=

530my)

PO-D Ach. 4X PO-D Ach. 10X PO-D Ach. 20X (spring) PO-D Ach. 40X (spring) PO-D Ach. 100X (spring, oil)

PO-D Plan 4X PO-D Plan 10X PO-D Plan 20X (spring) PO-D Plan 40X (spring) PO-D Plan 100X (spring, oil)

WHK 10X

Cross-WH Micro-WH

K

1 OX 1 OX

-------__-

BHSP-F

BH2-TR30

BH2-PA

BH2-SRP

AH-TP147

AH-TP530

BHS-LSH

Model

651P

1

1 1

-

1 1

1

2

1 1

1 1 1 1

-

1 1 1

-

653P

1

1 1

-

1

2 1

-

1 1 1 1 1

1 1 1

-

BHS-

751P

1

-

1 1

1

2 1 1

1 1 1 1

-

1 1 1 1

753P

1

-

1 1

1

2

1

-

1 1 1 1 1

1 1 1 1

Optional Accessories: Berek compensator AH-CT P-3 Attachable mechanical stage AH-FMP

11.

NOMENCLATURE

The Model

Revolving

nosepiece

BHSP

consists of various components as shown in the photo below:

Observation tube

,

Objective

Stage

Condenser

Base

Ill.

ASSEMBLY

This picture illustrates the sequential procedure of assembly. The numbers indicate the order of assembly Take care to keep all glass surfaces clean, and avoid scratching the glass surfaces.

of

various components. Remove dust caps before mounting components.

NOTE:

Quarter wave plate

Sensitive wave plate

**Berek compensator

**Mechanical

For numbers

page.

0,

0

and 0 please refer to explanations in detail on the next

Observation tube

Condenser

*

Screw the

**

Optionally available.

10X

Collector lens

Microscope stand

objective into theJfixed aperture of the nosepiece.

Explanations

@

Mounting the halogen bulb

1)

Releasing the bulb clamping levers @ of the collector lens the arrow, insert two contact pins of the

halogen bulb into the socket

(Recommended to use a glove or gauze to handle the halogen bulb.)

2)

Secure the bulb in position with the two

levers.

*

Before use, wipe off any fingerprints or stains on the bulb.

0

Mounting the stage

1) Prior to mounting the stage, rack down the stage mounting dovetail

way. (Fig.

2)

Loosen the stage clamping screw @ by

rotating counterclockwise with Allen wrench provided. (Fig.

Insert the stage into the mounting dove-

tail

a

3)

Lower the stage until it comes in contact

with the stop pin screw

in

detail

a

in the direction of

2)

from above slowly. (Fig.

0;

then clamp with

@

.

(Fig.

2)

@.

a

2)

(Fig.

all the

1)

Fig. 1

Fig.

2

0

Mounting the revolving nosepiece

1)

Loosen the nosepiece clamping screw (Fig.

3)

2)

Aligning the nosepiece dovetall slide to the mounting block

piece

slowly all the way

*

Do

inserting into the mounting block.

Mounting the intermediate polarizing attachment

1)

Loosen the clamping screw

spring-loaded clamping screw will cause the locating pin draw. (Fig. the screw further until the pin withdraws.

2)

With clamping.screw 0 pulled out, insert the circular dovetail of the intermediate attachment into the ring dovetail.

3)

Tighten the clamping screw.

not

tilt

or lock the nosepiece while

4)

@,

push in the nose-

a

fully. Pull

@.

@

to with-

If the pin does not, loosen

a

This

Fig.

3

!

Fig.

4

IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS

IV.

1,

!

Light path selector

The knob can be operated in 3

positions to deflect the light

as desired.

knob

Field

iris

diaphragm

Arrow mark cates increase in diaphragm intensity. vided. diameter.

@

ring

-t

0

indi- For continuously variable light Accepts the filter holder pro-

Diopter adjustment ring

Photo tube

45'

click

stop lever

Stage centerinq knob

Pre-focusing lever

adjustment knob

P

ertrand lens turret ring

Engraved letters "IN" for insertion of Bertrand lens into the liqht path. removal of. the Bertrand lens from the light path.

Bertrand lens focusing ring

'.

Clamping screw for stage rotation

Polarizer clamping screw

After optimum extinction is attained, clamp the polarizer.

"OUT"

for

Swing-out knob for lens

Condenser

(when top lens swings

out, N.A. is

N.A.

0.25).

is

0.9

top

/

Y

Main switch

holder*

Line cord

*Do not replace filters for about a few minutes after use, in order to give time to cool.

**The circuit breaker protrudes its central part to cut off the electrical power in case of the

dimmer circuit trouble (due to short circuit, etc.) or overcurrent. To restore the breaker, press the central part. If the breaker is actuated again, disconnect the line cord from the

AC

outlet

and contact the Olympus service center.

Summary of Putting the Microscope in Operation

Model BHSP

2

@

+

0

Match the line voltage selector switch to local mains voltage (see page Switch on the light source. Place a specimen slide on the stage. Remove the Bertrand lens and analyzer from the light path.

10X

Coarse focus with the Make interpupillary and diopter adjustments (page Set the analyzer to optimum extinction position (page Center the condenser (page Center the stage (page Center objectives other than

J

.

Swing in the desired objective.

K.

L.

Set the condenser, analyzer and Bertrand lens correctly according to your microscopic purpose (pages

M. Fine focus.

Adjust aperture iris diaphragm and field iris diaphragm (page

N.

15

and

Microscopic

application

1

orthosco~ic

observation

objective.

10).

11).

12).

13). 10X

(page

13).

16)

/

Adjustment of Illumination System

Bertrand lens

Objective

/

4X

to

loox

in intermediate polarizing at-

tachment

I

OUT

13).

Condenser top lens

I

OUT

10).

i

1

Conoscopic

observation

Generally for biological use, however, remove the analyzer, Bertrand lens and test plates from the light path.

*

Cut off this page at dotted line and put reminder of microscopic procedure.

20X

to

100X

IN

I

it

on the wall near the microscope for use as a

IN

OLYMPUS

V.

OPERATION

Switching on the Light Source

A.

1) Ascertain that the voltage selector switch is set to conform with the local mains volt-

age. (Fig. set, adjust it by means of the Allen wrench provided or a screwdriver.

2)

Place the sliding voltage control lever @ on

the right side of the microscope base to a position closest to you (low voltage position)

(Fig.

3) Actuate the main switch

(

Voltage Adjustment and Light Intensity

As you push the control lever

rection of the arrow in order to obtain in-

creasing intensity (Fig.

@

will display the lamp voltage.

Two LEDs on the left side indicate the volt-

age from right side from ments. The indication with the letters

"PHOTO"

color photomicrography.

6)

5)

If the switch is not correctly

@.

(Fig.

a

6),

the LED readout

OV

to

6V,

and twelve LEDs on the

6.5V

to 12V in

can

be used as a guide line for

0.5V

@

5)

/

in the di-

incre-

Fig.

5

-

6

B.

Adjusting the Observation Tube

1) At the time of inserting the eyepieces into

the observation tube, take care to insert the eyepiece with cross hairs or micrometer of your choice into the right eyepiece tube, aligning the positioning slot

ing pin

2) Looking through the both eyepieces with both eyes, adjust the interpupillary distance, sliding the knurled dovetail slides of the

right and left eyepiece tubes, until perfect

binocular vision is obtained. (Fig.

3) Looking through the right eyepiece (with cross hairs or micrometer) with your right eye, rotate the upper helicoid ring

eyepiece until the cross hairs (or micrometer)

are sharply focused. (Fig.

4)

Focus on the specimen with the coarse and

fine adjustment knobs so that the sharp

images of the specimen and cross hairs (or micrometer) can be obtained simultaneously.

5)

Now look at the image through the left eyepiece with your left eye and rotate the diopter adjustment ring specimen without using the coarse and fine adjustment knobs. (Fig.

@.

(Fig.

7)

a

and position-

8)

Q)

8)

@

to focus on the

8)

of the

Fig.

7

8

[

Light Path Selection

The trinocular tube is provided with a light

a

path selector knob the observation tube and/or to the photo tube in

3

positions. (Fig.

to direct the light to

9)

Knob position

I

Amount of light

I

Application "Crossed filter" (1 ) Normal

An indicator plate is provided at the knob port to summarize the usage of the above table;

it

can be consulted before operating the knob.

V: Viewer (white letter) C.V: Camera and viewer (yellowish green letters) C: Camera (red letter)

The colors of the letters correspond with the color bands on the knob shaft.

Use

C.

2)

3)

4)

5)

6)

of the Orientation Plate

The analyzer built in the intermediate attachment should be adjusted for optimum extinction by means of the orientation plate provided in the following steps:

1) Bring the 10X objective into the light path, and make sure that the red dots intermediate attachment and microscope stand are aligned. (Fig. 10)

Set both polarizer and analyzer at position "0" to attain the "crossed filter" position Place the orientation plate on the center of the stage.

Looking at the orientation plate through the eyepieces, rotate the stage (as you rotate the

stage, the orientation plate darkens and brightens alternately) until it most darkens or attains the extinction position; then, touch up the position ot the orientation plate manually so that the lower edge (fiducial line) of the orientation plate nears the cross line

Disengage the analyzer @ from the light path, which makes the field of view bright.

Loosening the observation tube clamping screw @ , until the fiducial line of the orientation plate is in parallel with the cross line; then, reclamp the observation tube. (Fig. 10)

Pushed in all the way

1

100% into binocular tube 1 20% into binocular tube / 100% into photo tube

(V) (C.V)

1 1

observation

Pulled out halfway

80% into photo tube

/

observation

(2)

Photomicrography

(focusing through

the binocular tube)

rotate the observation tube slightly

Pulled out all the way

(C)

I I

1

Photomicrography

a

(X

-

Fig.

Orientation

Plate Cross line

'10

I

1)))

on both

axis).

0

Fiducial line

parallel

D.

Centering the Condenser

1) Bring the objective 10X into the light path.

*

If a specimen is placed on the circular rotatable stage without a mechanical stage recommended to hold the peripheries of the

specimen with the stage clips provided.

2) Swing in the condenser top lens, and bring the specimen into focus.

3)

Stop down the field iris diaphragm slightly blurred image of the field diaphragm can now be seen in the eyepiece. (Fig. 1 1

4)

Adjusting the condenser height, focus on the

image of the field diaphragm.

*

If the specimen slide is too thick, it is sometimes impossible to obtain a sharply-focused

Image.

5)

While widening the diameter of the field progressively, use the condenser centering screws to bring the diaphragm image into the center of view. (Fig. 11)

it

O.

A

is

)

Fig.

11

@

6)

Push analyzer make sure that both polarizer and analyzer are set at position filter" position. Then loosen the clamping screw

@

7)

Remove the specimen out of the light path

SO

that a transparent area comes into the

light path. Keeping the polarizer at the

position, rotate the polarizer rotation ring

@

until the optimum extinction is obtained,

then clamp the ring. (Fig. 12)

*

Make sure that no test plate

a

into the light path, and

"0"

to attain the "crossed

of the polarizer. (Fig. 12)

is

engaged.

"0"

5

..

--.

7773?w-,*

Fig.

12

E.

Centering the Stage

1) Place a specimen on the stage, and insert two centering wrenches

into the stage center-

ing screws (Fig. 13). Looking through the eyepiece and objective IOX, fix your eyes on

some particular point, for instance, at the point A or the center of the cross hairs.

2)

As you rotate the stage, the particular point in the specimen image moves f rom the point

in a circular path (A circular path

E.

+ B + C +

(Diag. a)

D + A or A

+ D + C + B +

A) around the center of the

3) When the stage is turned by 180° from the point A, the particular point coincides with the point C.

4) Coincide the particular point to the point

E by means of the two centering wrenches (Fig. 13). (Diag. b)

5)

Next, move the particular point from the point

E

to the center A of the cross hairs

(Diag.

c).

Ir

Repeat this procedure until the stage centra-

tion is complete. After completing the cen-

Fig. 13

tration, remove the wrenches.

Diag. a

Diag.

b

Diag.

c

A

F.

Centerins the Obiectives

This objective centration is necessary to all the PO objectives except P010X. After completing the stage centration, insert two centering wrenches

a

into the objective centering screws provided at each centerable objective aperture

in

the nosepiece. (Fig.

14) The other procedure is just the same as

with stage centration.

G.

Use of Iris Diaphragms

Fig. 14

When the top lens of the polarizing condenser is swung out for orthoscopic observation, the aperture iris diaphragm serves as a field iris diaphragm and the field iris diaphragm as an aperture iris diaphragm.

For conoscopic observation, generally the aperture iris diaphragm

is

fully opened and the field iris diaphragm can be effectively used for reduction of glares and conoscopicobservation of very small objects.

1)

Aperture iris diaphragm Adjust the opening of the aperture iris diaphragm according to the various conditions such as the numerical aperture of the objective, image contrast, depth of focus, and flatness of field. Generally it is often preferable to

stop down the aperture iris diaphragm to about

80%

of the N.A. of the objective. After the eyepiece is removed from the observation tube, if necessary, look through the observation tube and check the opening of the aperture diaphragm at the objective pupil.

2) Field iris diaphragm The field iris diaphragm controls the diameter of the ray bundle impinging on the specimen surface and thus increases image definition. Generally, it is preferable to slightly increase the diameter of the field iris diaphragm until

it is just outside the field of view.

H.

Focus Adjustment

1.

Tension of Coarse Adjustment Knobs and Fine Adjustment Although the tension of the coarse adjustment knobs has been already adjusted for optimum performance by the manufacturer,

it is possible to personally adjust the tension of the coarse adjustment for either heavy or

light movement depending on the operator's preference by rotating the tension adjust-

ment ring The ring can be rotated by inserting a screwdriver into one of the holes on the periphery of the ring. The clockwise rotation (in the direction of the arrow) tightens the coarse adjust-

ment knobs. Do not loosen the ring too much, because the stage may drop or the fine

adjustment knobs may slip.

NOTE: Do not rotate the right and left coarse adjustment knobs in the opposite directions

a.

(Fig.

15)

simultaneously. If the stage drops and the specimen goes out of focus, the tension adjustment ring is too loose. Tighten the ring.

70%

or

-.

Fig.

15

70-80%

30-

20%

2. Pre-focusing Lever This lever

contact between specimen and objective as well as to simplify coarse focusing. (Fig. The lever is locked after coarse focus has been accomplished. This prevents further upward travel of the stage by means of the coarse adjustment knobs, and automatically

provides a limiting stop if the stage is low-

ered and then raised again. The pre-focusing

lever does not restrict fine focusing.

@

is provided to prevent possible

16)

Fig.

16

3.

Adjustment of Stage Block Height

In addition to the vertical movement of the stage by means of coarse and fine adjustments, the stage block height can be changed for observation of specimens which are thicker than standard slides. To lower the stage block;

1)

Loosen the stage block locking screw with Allen wrench provided, and raise the stage block until the stopping screw

a

can be seen, then reclamp.

2)

Replace the stopping screw into the lower threaded hole@). (Fig. 17)

3)

Unclamping the stage block again, lower until it stops, and clamp.

I.

Use of Immersion Objectives

1) Focus the specimen with a low power objective.

2)

Put a drop of immersion oil on the specimen slide and the front lens of the immersion objective.

3)

Turn the revolving nosepiece to bring the immersion objective into the light path, and focus with the fine adjustment knobs.

NOTE:

a

Care should be taken to prevent oil bubbles from forming in the oil film If any, re-apply immersion oil, for these bubbles greatly deteriorate the lens performance.

a

After use carefully wipe off the immersion oil deposited on the lens surfaces with gauze moistened with xylene. Never leave oil on the lens surfaces after use as oil remnants will seriously impair the performance of the lens system.

a

Fig. 17

'

4

1

J.

Orthoscopic Observation

1)

Swing out the top lens of the condenser.

In principle, polarized light enters the light path, parallel to the optical axis, to enable observation of the optical characteristics of the specimen. However, this method will darken the field of view and lower the resolving power of the objective extremely. Therefore, swing out the top lens of the condenser, using only the lower aperture of the lower condenser lens (N.A.

2)

Insert the analyzer into the light path, and attain the crossed filter position with analyzer and polarizer at this position, the polarizer vibration the

X

direction, and the analyier vibra-

tion in the

position, pull out the analyzer rotation

screw.

3)

Rotate the stage until the extinction of

the image is attained, and move the

click stop lever

(Fig. 18)

0.25).

0

setting. At

Y

direction. To open the filter

a

toward the operator.

is

45"

in

Fig. 18

From this position, it is easy to rotate the stage in refer to the angular scale, and the stage clicks at the diagonal position, at which position, the retardation angle is measured. (Take care if you rotate the stage too fast, it sometimes overrides the click stop position.) To release the

45'

click stop lever.

4)

Insert the quarter wave plate or sensitive tint plate into the slot, closest to you at your right hand side in the intermediate polarizing tube. To disengage the test plate, you can just pull

*

A

Berek compensator is optionally available to measure the birefringence of a specimen.

it

45'

increments without having to

45'

click stops, push back the

back to its click stop position.

Sensitive tint plate Quarter wave plate

K.

Conoscopic Observation

1)

Swing in the top lens of the condenser

need to immerse between the condenser and specimen slide.

2)

Bring the specimen into focus, rotate the Bertrand lens turret ring into the IN position.

3)

Focus on the interference figure formed at the back focal plane of the objective from

20X

to

100X.

objects.) The pinhole cap provided may be used in place of the eyepiece to directly view the

interference figure mentioned above. In this case, the Bertrand lens is disengaged.

L.

Photomicrography

1)

Photomicrographic equipment

Photomicrography with the Model BHSP requires photomicrographic equipment such as the photomicrographic system camera, exposure meter, photo eyepiece, etc. Read the instruction manuals for each equipment.

2)

Photo eyepieces graphy, and

Berek compensator

(N.A.

0.9).

and illuminate the specimen with no

(Recommended to stop down the field irisdiaphragm in case of very small

NFK3.3X

NFK2.5X

and

NFK5X

for conoscopic photomicrography.

are recommended for orthoscopic photomicro-

3)

Image magnification is obtained from the equation below:

Objective magnification

X N FK

photo eyepiece magnification.

VI.

OPTICAL DATA

Objective

\

Eyepiece

\

WHKlOX

(Field number

WHKlOX

(Field number

Magnificat ion

W.

D.

(mm)

Focal length (mm) Resolving power (p) Total magnification

Focal depth

20)

Field of view dia-

f

meter (mm)

+

Magnification

Focal length (mm) Resolving power (p)

Total magnification

Focal depth

20)

Field of view diameter (mm)

(p)

1

34.23

3.36 40X

172.5 5

1

1.34

1

OOX

27.6

2

PO

D

Ach.

PO D Plan

20X

0.4

0.83

8.99

0.84

200X

9.1

4

1

+

40X

0.65

0.23

4.67

0.52

400X

3.0

0.5 0.1

1 OOOX

1

OOX

1.25

0.17

1.75

0.27

1 OOOX

0.68

*

Immersion objective. Resolving power is obtained when the objective is used at the full

aperture diaphragm.

0

W.D. (Working distance): The distance between the specimen or cover glass and the nearest point of the objective.

0

N.A.

(Numerical aperture):

The numerical aperture represents a performance number which could be compared to

the relative aperture (f-number) of a camera lens.

comparing the resolving powers of all types of objectives. The larger resolving power.

0

Resolving power:

The ability of a lens to register small details. The resolving power of a lens is measured

by its ability to separate two points.

0

Focal depth: The distance between the upper and lower limits of sharpness an optical system.

0

Field number:

A

number that represents thq diameter in mm of the image

is formed by the lens in front of it.

0

Field of view diameter: The actual size

of

the field

of

view in mm.

N.A.

values can be used for directly

N.A.,

the higher the

in

the image formed by

of

the field diaphragm that

VII. TROUBLESHOOTING

If you are unable to obtain full performance from your microscope, please consult with the

table below as pointers for troubleshooting.

Troubles

1.

Optical System

(a) With illuminator

switched

field of view cannot be seen.

(b) Field of view is

cut off or illumi-

nated

(c) Dust or dirt is

visible in field of

(d) Excessive image

contrast.

(e) Resolution prob-

lems:

0

Image is not sharp.

0

Insufficient con-

t rast.

o

lmage details lack

definition.

On,

irregularly.

Causes Remedies

Bertrand lens is engaged.

Analyzer and polarizer are in "cross

filter,n

position

Light path selector lever is stopped

midway. Nosepiece is not click stopped.

Nosepiece

to stand.

Condenser is not correctly mounted on ring mount.

Test plate is stopped midway.

In case of orthoscopic observation, condenser top lens stays in light path or stops midway.

Field iris diaphragm is stopped down excessively.

Lamp is not correctly attached. Dust or dirt on glass surface at light

exit on base. Dust on condenser top lens. Dirty specimens. Dust on eyepiece. Condenser is lower excessively. Aperture iris diaphragm

down excessively. Nosepiece is not correctly attached.

Objective is not correctly position- Slightly rotate nosepiece until it ed in light path.

I

Dirt on objective front lens.

Immersion objective i$ used with-

out immersion oil.

Bubbles in immersion oil.

Olympus designated oil is not used.

Dirty specimen.

Dirt on condenser lens.

Specimen is not properly illumi- Adjust illumination.

nated.

is

OjJ,,

not correctly attached

is

stopped

Disengage. Disengage analyzer.

V.

or

Push in lever up to C.

Slightly rotate nosepiece until it clicks into position.

Insert sliding dovetail mount kto stand all the way, until it stops, then lock.

Re-insert condenser all the way.

Push plate all the way until it clicks. Swing it out of light path.

Open diaphragm fully.

Re-insert lamp correctly. Clean off dust or dirt.

Raise condenser. Open diaphragm.

Insert sliding dovetail mount all

it

the way, until

clicks into position.

I

Clean objective. Apply immersion oil.

Remove bubbles.

Use designated oil. Clean.

stops, then lock.

V.

1

Troubles

I

Causes

I

Remedies

i

(f) Field of view

is partially out

of focus.

of focuseccentrically.

(h) Light intensity

does not increase

although voltage is raised.

(i) No conoscopic

image can be seen.

(j)

Crossed filter position is not

attained.

Nosepiece

Objective is not correctly positioned in light path.

Specimen is not correctly positioned on stage.

Nosepiece is not correctly attached.

Objective is not correctly positioned in light path.

Condenser is out of center. Condenser is not correctly centered. Condenser is lowered excessively.

Condenser top lens is not in light path.

Analyzer is out of light path.

is

not correctly attached.

Insert sliding dovetail mount into

stand all the way, then lock. Slightly rotate nosepiece until

clicks into position. Place specimen on stage and secure

it

with specimen clips.

Insert sliding dovetail mount all the

it

way, until Slightly rotate nosepiece until it

clicks into position. Center condenser. Center condenser. Raise condenser.

I

Swing it in.

Push it in.

stops, then lock.

it

2.

Electric System

(a) Illuminator

too bright (or

too dark).

(b) Output voltage

for illuminator

cannot be regu-

lated.

(c) Lamp flickers

and intensity is unstable.

(d) Reduced bulb

life.

is

Line voltage selector switch matched to the mains voltage.

Mains voltage is too high (or too low).

Voltage selector switch is not matched to mains voltage.

Mains voltage is too low or too high.

is

Mains voltage

-

Loose electrical connection. Bulb is not a standard bulb. Use a standard bulb.

unstable.

is

Match selector switch with mains

not

voltage.

Adjust mains voltage with a variable

voltage transformer. Adjust mains voltage selector switch

to mains voltage.

Adjust mains voltage with a variable voltage transformer.

Use a variable voltage transformer.

Secure connection.

1

Troubles

Causes

Remedies

(a) Coarse adjust-

ment is too tight.

(b) Stage drops or

specimen goes out of focus.

(c) Stage cannot be

raised to upper

limit.

(d) Stage cannot be

lowered to lower limit of working

range.

(e) Objective front

lens hits against specimen.

4.

Observation Tube

(a) Incomplete

binocular vision.

Tension adjustment ring

ed too much.

User is trying to raise stage passing over upper focusing limit imposed by engaged pre-focusing lever.

Tension adjustment ring is too

loose.

Pre-focusing lever is engaged in

lower than focusing position.

Condenser mount is lowered too much.

Specimen is mounted on stage upside down.

lnterpupillary distance is not cor-

rectly adjusted.

Diopter adjustment is incomplete.

is

tighten-

Loosen tension adjustment ring

proper1 y.

Unlock pre-focusing lever.

Tighten ring properly.

Unlock pre-focusing lever

Raise condenser mount.

!

Reverse specimen.

Correct interpupillary distance.

Complete diopter adjustment.

J

<

_i

/

5.

Stage

out of focus when you touch

stacle.

(b)

Specimen stops midway on the

X

or Y traverse.

(c) When stage is

rotated, image of specimen goes out of field of

1

view.

Right and left eyepieces are not

matched.

User is unaccustomed with a binocular vision.

Stage

is

not correctly clamped.

Specirnen is not corre'ctly posi-

tioned on stage.

Stage is not centered or objective

is not.

Use a pair of rriatched eyepieces.

Prior to looking at the image of specimen, try to look entire field of view, or look at a far away object before resuming microscopic observation.

Clamp stage securely. (a) l mage easily goes

Adjust specimen position.

Center.

Olympus BH2 BHSP User manual [gb]

Specifications and Main Features

Frequently Asked Questions

User Manual

CONTENTS

STANDARD EQUIPMENT

NOMENCLATURE

ASSEMBLY

IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS

Summary of Putting the Microscope in Operation

Adjustment of Illumination System

OPERATION

Switching on the Light Source

Adjusting the Observation Tube

Centering the Condenser

Centering the Stage

Use of Iris Diaphragms

Focus Adjustment

Use of Immersion Objectives

Orthoscopic Observation

Conoscopic Observation

Photomicrography

OPTICAL DATA