H301-NIKON-TI-SR User Manual
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Compatible with the following XY stages
Compatible with the following Okolab Controllers
Nikon T/Ti-SR
H301-T-BL-PLUS
UNO-COMBINED-CONTROLLER
H401-T-DUAL
March 2015
H301-NIKON-TI-SR
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H301-NIKON-TI-SR User Manual
Page2
March 2015
Index
1. Components and dimensions................................................................................................... 3
2. Sample Holders ................................................................................................................... 3
2.1 Available Sample Holders ....................................................................................................... 3
3. Available Lids ..................................................................................................................... 4
3.1 Sliding lid .......................................................................................................................... 5
3.2 Koehler Lid ........................................................................................................................ 6
3.3 Injection Lid ...................................................................................................................... 6
3.4 Laser Interlock Lid ............................................................................................................... 7
4. Insertion of the Sample Feedback Temperature Sensor .................................................................. 8
5. Insertion of Sample Holder into Chamber ................................................................................... 8
6. Working with 35 or 60 mm Petri Dish – Spacing Rings and Magnetic Locks ........................................... 9
7. Working with 1x3’’ and 1x2’’ chamber slides - magnetic locks ........................................................11
8. Connecting the Gas Supply and Filling the Humidity Module ...........................................................12
9. Working with Perfusion ........................................................................................................12
10. Connecting the Chamber with XY stage ....................................................................................13
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H301-NIKON-TI-SR User Manual
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Distance between focal plane
and upper side of the glass lid
Chamber top view
Sliding Lid
Chamber Base
Sample Holder-Order Separately
Integrated Humidty Module
1x35-M single
#1 35mm Petri-dish
1xGS-M single
#1 1x3in. chamber slide
1xLABTEK-M single
#1 Lab-Tek 1in.x2in. chambered cover glass
1xLABTEK-II-M single
#1 Lab-Tek II 1in.x2in. chambered cover glass
1x60-M single
#1 60mm Petri-dish
March 2015
1. Components and dimensions
H301-NIKON-TI-SR includes the following components:
Chamber main body with embedded temperature sensor
Sliding heated glass lid with Indium Tin Oxide (ITO) conductive coating and embedded temperature sensor
Integrated humidity module. It is not necessary to select an additional humidity module
Figure 1. H301-NIKON-TI-SR - Components and Dimensions.
2. Sample Holders
2.1 Available Sample Holders
The following sample holders are available.
NOTE: Please contact info@oko-lab.com if you cannot find the sample holder you are looking for. We are constantly
adding new inserts to the list.
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H301-NIKON-TI-SR User Manual
Page4
1x35-M single
For #1 35mm Petri-dish
1x60-M single
For #1 60mm Petri-dish
1xGS-M single
For #1 1’’x3’’ chamber slide
1xLABTEK-M single
For #1 Lab-Tek 1’’x2’’ chambered cover glass
1xLABTEK-II-M single
For #1 Lab-Tek II 1’’x2’’ chambered cover glass
March 2015
Figure 2.Available sample holders.
3. Available Lids
The following heated glass Lids are available for H301-NIKON-TI-SR :
H301-SLIDING-LID: For easy sample loading and pipetting – Included in code H301-NIKON-TI-SR
H301-KOEHLER-LID-2*: Reduces chamber height to 23 mm and allows imaging under Koehler illumination
H301-INJECTION-LID-2*: Heated glass lid with two small openings (sealed with flexible plastic) allowing injec-
tion or permanent access to the sample. Compatible only with sample holder 1x35
H301-LASER-INTERLOCK-LID-2*: Heated glass lid with safety switch. Connects to laser controller and auto-
matically turns laser off when lid is lifted. Compatible only with sample holder 1x35
* OPTIONAL – not included with H301-NIKON-TI-SR
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