Operating Manual
and
User’s Guide
CHEM2000 and CHEM2000-UV-VIS
Miniature Fiber Optic Spectrophotometers
Ocean Optics, Inc.
380 Main Street
Dunedin, FL 34698
(727) 733-2447
(727) 733-3962 fax
For the latest information, consult our web site:
www.OceanOptics.com
Or, e-mail our Technical Service Department:
TechSupport@OceanOptics.com
021400
Copyright © 2000 Ocean Optics, Inc.
All rights reserved. No part of this publication may be reproduced,
stored in a retr ieval system, or tr ansmitted, by any means, elect r onic,
mechanical, photocopying, recording, or otherwise, without written
permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it
shall not, by way of trade or otherwise, be lent, re-sold, hired out or
otherwise circulated with out the prior consent of Ocean Optics, Inc. in any
form of binding or cover other than that in which it is published.
Trademarks
Microsoft, Windows, Windows 95, Windows 98 and Windows NT are
either registered trademarks or trademark s of Microsoft Corp or ation.
Limit of Liability
Every effort has been made to make this manual as complete and as
accurate as possible, but no warranty or fitness is implied. The information
provided is on an “as is” basis. Ocean Optics, Inc. shall have neither
liability nor responsibility to any person or entity with respect to any loss or
damages arising from the information contained in this manual.
Table of Contents
Introduction
Quick Start
Installing the PC2000 and OOIChem Software
Using OOIChem Software
Display Functions
Spectrometer Channel Selection............................................................................................. 10
Mode of Operation................................................................................................................... 10
Cursor Function Bar ................................................................................................................11
Text Box ..................................................................................................................................12
Acquisition Parameters ........................................................................................................... 12
Reference Scan....................................................................................................................... 12
Dark Scan................................................................................................................................ 12
Subtract Dark........................................................................................................................... 13
Acquire Data Modes................................................................................................................13
Scaling the Graph.................................................................................................................... 13
File Menu Functions
Save Spectral Values..............................................................................................................14
Save Kinetics Values............................................................................................................... 14
Open Spectrum Overlay..........................................................................................................14
Open Kinetics Values .............................................................................................................. 14
Printer Setup............................................................................................................................ 14
Print Spectra and Kinetics.......................................................................................................14
Exit...........................................................................................................................................14
Edit Menu Functions
Clear Spectrum Overlays ........................................................................................................ 15
Clear Kinetics Values .............................................................................................................. 15
Autoscale X ............................................................................................................................. 15
Autoscale Y .............................................................................................................................15
Show Kinetics Values..............................................................................................................15
Show Legends......................................................................................................................... 15
Spectrometer Menu Functions
Scan.........................................................................................................................................16
Select Concentration Wavelength...........................................................................................16
Calculate Calibration Curve.....................................................................................................16
Enable Strobe.......................................................................................................................... 16
Spectrometer Configuration .................................................................................................... 16
Kinetics Configuration..............................................................................................................18
Hardware Configuration........................................................................................................... 18
................................................................................................................................1
..................................................................................................................................4
............................................................ 6
.................................................................................................... 9
.................................................................................................................. 10
............................................................................................................. 14
............................................................................................................. 15
........................................................................................... 16
CHEM2000 Sampling Components
ISS-2 Integrated Sampling System
400-
m Diameter Optical Fiber
µµµµ
CHEM2000-UV-VIS Sampling Components
ISS-UV-VIS Integrated Sampling System
300-
m Solarization-resistant Optical Fiber
µµµµ
................................................................................ 19
................................................................................... 19
.......................................................................................... 22
................................................................ 23
........................................................................ 23
................................................................... 26
Experiment Tutorial
Absorbance Experiments
.............................................................................................................. 27
.................................................................................................... 27
Transmission Experiments
Reflection Experiments
........................................................................................................ 29
Relative Irradiance Experiments
Concentration Experiments
Kinetics Experiments
........................................................................................................... 33
................................................................................................. 28
....................................................................................... 30
................................................................................................ 31
Appendix A: Changing the Settings on the PC2000
Appendix B: PC2000 Pin-outs and Jumpers
.............................................................. 36
............................................... 34
Appendix C: Calibrating the Wavelength of Your Spectrometer
....................... 37
Introduction
Ocean Optics miniature fiber optic spectrometers and accessories have revolutionized the analytical
instrumentation market by dramatically reducing the size and cost of optical sensing systems. More than
10,000 Ocean Optics spectrometers have been sold worldwide -- striking evidence of the far-reaching
impact of low-cost, miniature components for fiber optic spectroscopy. Diverse fields such as research and
development, industrial process control, medical diagnostics, environmental monitoring and o f course,
education have benefited from access to Ocean Optics technology.
In fact, Ocean Optics has its roots in education. It formed in 1989 when Florida university researchers
developed a fiber optic pH sensor as part of an instrument designed to study the role of the oceans in global
warming. The researchers soon formed Ocean Optics, Inc. and their ingenious work earned a Small
Business Innovation Resea rch grant from the U.S. Department of Energy. While d esigning the pHmonitoring instrument, the researchers wanted to incorporate with their sensor a spectrometer small enough
to fit onto a buoy and were surprised to discover none existed. So, they built their own.
In 1992, the founders of Ocean Optics filled a substantial need in the research community and changed the
science of spectroscopy forever by introducing a breakthrough technology: the S1000 Miniature Fiber
Optic Spectrometer, nearly a thousand times smaller and ten times less expensive than previous systems.
Due to this dramatic reduction in size and cost of optical sensing systems, applications once deemed too
costly or impractical using conventional spectrometers were now feasible.
CHEM2000 and CHEM2000-UV-VIS Spectrophotometer Systems
The CHEM2000 and CHEM2000-UV-VIS Spectrophotometer Systems utilize the optical bench of our
second-generation miniature fiber optic spectrometer, the S2000, by mounting it onto an A/D converter and
turning the system into a PC plug-in spectrometer.
CHEM2000
The CHEM2000UV-VIS also consists of four basic elements:
the PC2000-UV-VIS PC Plug-in Fiber Optic Spectrometer
(200-850 nm), a miniature deuterium tungsten light source
with integrated cuvette holder, a 300-µ
resistant optical fiber, and OOIChem operating software.
The light source supplies light to the sample. The light
transmitted through the sample is collected and sent to the spectrometer via the fiber. The spectrometer
measures the amount of light at each wavelength in the sampled spectrum. The A/D converter, on which
the spectrometer is mounted, transforms the analog data from the spectrometer into digital information that
is passed to a computer. Finally, the software performs basic acquisition and display functions on your data.
m solarization-
The CHEM2000 consists of four basic elements: a
PC2000 PC Plug-in Fiber Optic Spectrometer (350900 nm), a tungsten-halogen light source with
µ
integrated cuvette holder, a 400OOIChem operating software.
m optical fiber, and
CHEM2000-UV-VIS
PC2000 PC Plug-in Fiber Optic Spectrometer
The PC2000 is 2048-element linear CCD-array fiber optic spectrometer mounted on a 1 MHz A/D card that
fits easily into an ISA-bus slot in the PC. The spectrometer has a 25-µ
nm (FWHM). The 12-bit, 8-channel A/D card is single-ended and half-length. Though the standard
CHEM2000 and CHEM2000-UV-VIS are single-channel systems, additional slave spectrometer channels
can be purchased at any time to expand wavelength range or perform multiple tasks.
m slit and provides a resolution of 1.5
Light Source/Sample Holder
The ISS-2 Integrated Sampling System that comes with the CHEM2000 is a versatile light source/sample
holder with a tungsten-halogen bulb, a built-in filter slot for color-correcting and other uses and a sample
holder for 1-cm square cuvettes. The light source/sample holder is SMA-terminated for easy coupling to
optical fibers and has a 5-mm diameter f/2 collimating lens.
The ISS-UV-VIS Integrated Sampling System comes with the CHEM2000-UV-VIS. This light
source/sample holder has deuterium and tungsten bulbs, and a cuvette holder for 1-cm cuvettes. It occupies
7-1/2" x 4" and weighs <1 lb. The cuvette holder attaches directly to the light source and has a 5-mm
diameter f/2 collimating lens to collect the light and funnel it to the solarization-resistant optical fiber.
Optical Fiber
Included as part of the CHEM2000 is a 400-µm diameter, single-strand UV/VIS optical fiber that couples
easily via SMA terminations to the PC2000 and light source/sample holder. This silica-core, silica-clad
fiber is 2 meters in length. For the CHEM2000-UV-VIS, the included optical fiber is a 300solarization-resistant patch cord that carries light from the sample to the spectrometer.
µ
m diamter
OOIChem Spectrometer Operatin g Software
OOIChem is our standard Windows operating software for the CHEM2000 and CHEM2000-UV-VIS
systems. It is a basic acquisition and display software that provides a real-time interface to a variety of
spectral-processing functions. OOIChem allows users to perform basic spectroscopic measurements such as
absorbance, transmission, relative irradiance, and concentration. OOIChem operates with Windows 95,
Windows 98 and Windows NT. Visit our web site at www.OceanOptics.com/Software_Updates.asp to
download free OOIChem upgrades.
OOIBase32 Spectrometer Operating Software
OOIBase32 is our standard spectrometer operating software that we provide free of charge to all customers.
While OOIChem is a basic acquisition and display program, OOIBase32 is user-customizable and a much
more advanced acquisition and display program. With OOIBase32 you have the ability to control all
system parameters; collect data from up to 8 spectrometer channels simultaneously and display the results
in a single spectral window; perform reference monitoring and time acquisition experiments; and use
numerous editing, viewing and spectral processing functions. At any time, users can receive free
OOIBase32 updates from our web site at www.OceanOptics.com/Software_Updates.asp.
These are the standard components for the CHEM2000 and CHEM2000-UV-VIS systems. However, we
offer a complete line of light sources, sampling holders, in-line filter holders, flow cells, and other sampling
devices; an extensive line of optical fibers and probes; and collimating lenses, attenuators, diffuse
reflectance standards and integrating spheres. All components have SMA terminations so that changing the
sampling system is as easy as unscrewing a connector and adding a new component or accessory.
This modular approach -- components are easily mixed and matched -- offers remarkable applications
flexibility. Users pick and choose from hundreds of products to create distinctive systems for an almost
endless variety of optical-sensing applications
2
Packing List
A packing list comes with each order. It is located inside a plastic bag attached to the outside of the
shipment box. The invoice is mailed separately. The items listed on your packing slip include all of the
components i n your order. However, some items on your packing list are actually i t ems installed into your
spectrometer, such as the grating and slit. The packing list also includes important information such as the
shipping address, billing address, and components on back order.
Wavelength Calibration Report
In your shipment box, you will find your spectrometer in a silver-gray anti-static bag. Your software
diskettes and Wavelength Calibration Report are packaged with this manual. A Wavelength Calibration
Report comes with every spectrometer channel, contains important information specific to your
spectrometer, and should be retained. Use the calibration coefficients and intercept numbers on your
Wavelength Calibration Report to calibrate the wavelength of your spectrometer.
Upgrades
Customers sometimes find that they need Ocean Optics to make a change or an upgrade to their system. In
order for Ocean Optics to make these changes, the customer must first contact us to obtain a Return
Merchandise Authorization (RMA) number and to receive specific instructions when returning a product.
3
Quick Start
The CHEM2000 and CHEM2000-UV-VIS systems are easy to set up, allowing the user to start collecting
data within minutes. This Quick Start provides brief instructions on installing your PC2000, installing and
configuring the soft ware, and setting up your system. To find detailed directions on a specific component,
Table of Contents
see the
software instructions begin on page 9.)
Step 1: Interface the PC2000 to your PC
The PC2000 is our fiber optic spectrometer mounted onto an A/D converter. The entire instrument is
installed into your PC. But first you must find available Base Address and IRQ values.
1. The default settings for our A/D products are a Base Address (or Input/Output Range) of 768 decimal
and an IRQ of 7. You will need to match Base Address and IRQ settings on the A/D card to available
settings in your computer. First determine which settings are not being used by other hardware devices.
• If you have Windows 95/98, go to
icon. Choose the
devices. Under
Remember that these I/O settings are expressed in hexadecimal and correspond to our Base
Address, which is given in decimal, followed by the hexadecimal equivalent in parenthesis.
• If you have Windows NT, go to
NT Diagnostics
Select the
2. Note these available settings. When you first run OOIChem, you must enter these values in the
“Configure Hardware” dialog box.
3. Turn off the computer and take off the computer cover. Ground yourself to the computer chassis or
power supply and remove the PC2000 (spectrometer-A/D combination) from its static-shielded bag.
4. If necessary, change the position of the switches on the A/D converter. For the PC2000, there is only one
bank of switches on the A/D converter: the Base Address may be changed via the first 6 switches and the
IRQ may be changed via the last 3 switches. (See
5. Insert the PC2000 into an ISA-bus slot. Make sure the connections are snug and restart your computer.
. (Detailed PC2000 installation instructions begin on page 6. Detailed OOIChem
Device Manager
View Resources
. Click on the
I/O Port
button. Find an available I/O Range (Base Address).
Start | Settings | Control Panel
tab and double-click on “Computer” at the top of the list of
, note available settings -- numbers unassigned to hardware.
Start | Programs | Administrative Tools (Common) | Windows
Resources
tab. Select the
Appendix A
IRQ
on page 34 for switch positions.)
. Double-click the
button. Find an available IRQ.
System
Step 2: Install OOIChem Software
Before installing OOIChem, make sure that no other applications are running.
1. Insert “Disk 1” into your floppy drive. (When prompted, insert Disks 2 and 3.) Execute
2. At the “Welcome” dialog box, click
3. At the “Destination Location” dialog box, you can choose
Next>
Click
4. At the “Backup Replaced Files” dialog box, select either
you select Yes, you can choose
5. Select a Program Manager Group. Click
6. At the “Installation Complete” dialog box, choose
7. When prompted to do so,
.
Step 3: Configure OOIChem Software
After you restart your computer, navigate to the OOIChem icon and select it. Now that the PC2000 and
software ha ve been installed, you ne ed to configure your software. The first time you run OOIChem after
installation, you must follow several prompts to configure your system before taking measurements.
Next>
.
Browse
restart your computer
to pick a destination directory. Click
Next>
. At the “Start Installation” dialog box, click
Browse
Yes
Finish>
when the installation is complete.
4
.
to pick a destination directory.
or No. We recommend selecting Yes. If
Next>
Setup.exe
.
Next>
.
.
Hardware Configuration
Configure Hardware
The
box are usually set only once -- when OOIChem is first installed and the software first opens.
1. Under
2. Under
3. Under
4. Under
5. For your setup, only these parameters apply to your system. Click OK. You can always change these
Spectrometer Type
A/D Converter Type
Base Address
available setting you found in your computer). Remember that the Input/output Range (Base Address)
you selected was expressed in hexadecimal. In this dialog box, the base address is given in decimal,
followed b y the hexadecimal equivalent in parent hesis. For example, “
address as 768 decimal and 300 hexadecimal.
IRQ
, choose the same setting as the dip switches on the A/D converter (and the same available
setting you fou nd in yo ur computer).
settings once OOIChem is fully operational by selecting
dialog box opens when you first run OOIChem. The parameters in this dialog
, choose S2000/PC2000.
, choose ADC1000/PC2000.
, choose the same setting as the dip switches on the A/D converter (and the same
768 (0x0300)
Spectrometer | Hardware Configuration
” gives the base
.
Spectrometer Configuration
At this point, OOIChem should be acquiring data from your spectrometer. There should be a dynamic trace
responding to light near the bottom of the displayed graph. Now that OOIChem is running, you need to
configure your system. Select
Coefficients.
•
coefficients for your CHEM2000 or CHEM2000-UV-VIS under
Trigger mode.
•
spectrometer for synchronizing data with an external event.
Graph and chart display mode.
•
spectrometer channel. Choose
of the graph and to view a chart displaying your kinetics experiment in the bo ttom half of the graph.
Flash Delay.
•
VIS systems do not come with a strobe light source.
Color Temperature.
•
irradiance measurements. For CHEM2000 users, your lamp has a color temperature of 3100 Kelvin.
For CHEM20000UV-VIS users, your light source does not have a known color temperature.
From the Wavelength Calibration Report that came with your system, enter the
Select No E xternal Trigger, unless yo u have wired an external triggering device to the
This function is for use with a strobe light source. The CHEM2000 and CHEM2000-UV-
Spectrometer | Spectrometer Configuration
Choose
Spectrum & Kinetics
Enter the color temperature of your reference light source used in relative
Spectrum Only
to view both real-time live spectra in the top half
to only view live spectra from one
Master
from the menu.
.
Acquisition Parameters
Set data acquisition parameters by choosing an integration period and selecting averaging and boxcar
smoothing values.
Text Box
Enter the operator name, or any other identifying text here. This text appears in your data files. You can
edit this text at any time.
Step 4: Connect Sampling Optics
If you have a CHEM2000, take your 400-µm fiber that came with your system, screw one end into the
SMA connector on the light source and screw the other end of the fiber into SMA connector on your
PC2000, which you have already installed in your computer. If you have a CHEM2000-UV-VIS, take your
µ
m solarization-resistant fiber that came with your system, screw one end into the SMA connector on
300the light source and screw the other end of the fiber into SMA connector on your PC2000.
Step 5: Receive Data
Run OOIChem in Scope Mode and take a reference spectrum and a dark spectrum (see the
Tutorial
irradiance mode to take your sample measurements.
section beginning on page 27 for details). Choose the absorbance, transmission, or relative
Experiment
5
Installing the PC2000 and OOIChem Software
PC2000 PC P
The
spectrometer mounted onto an ADC1000 A/D card. The
analog-to-digital converter card that connects our miniature fiber optic spectrometers to desktop PCs. The
ADC1000 is a 12-bit, 8-channel, single-ended A/D card. This sturdy spectrometer-A/D card combination
fits easily into an ISA-bus slot in the PC. The following directions apply to both CHEM2000 and
CHEM2000-UV-VIS users.
Each device in or connected to your computer is assigned specific settings; it’s similar to giving each
device its own name so that your computer will know what to call and how to recognize the device. In
order for your PC2000 to work as a device in your computer, it has to be assigned a Base Address setting
and an IRQ setting. The default settings for each are:
Base Address (I/O Range) 768 decimal (300 hexadecimal)
IRQ (Interrupt Request) 07
These default values are set on the A/D converter. There are dip switches on the A/D converter and their
positions determine the values. These default values are set in the operating software as well. Most of the
time, these default settings will work with your computer. However, if you have many devices installed in
your computer, you may have a conflict; other devices may be using these settings. If there is a conflict
with another device in your computer, you must change the positions of the switches on the A/D converter.
For the PC2000, there is only one bank of switches on the A/D converter: the Base Address may be
changed via the first 6 switches and the IRQ may be changed via the last 3 switches. To first check your
computer to see which settings are available, follow the instructions for the Windows system that you use.
LUG-IN SPECTROMETER
has our 2048-element linear CCD-array fiber optic
ADC1000 A/D C
ARD
is a high-speed ISA-bus
Windows 95 and Windows 98 Users:
Find Available Base Address and IRQ Settings
1. Go to
2. Choose the
3. Under
Start | Settings | Control Panel
Device Manager
your computer.
View Resources
available settings for both the Interrupt request (IRQ) and the Input/output (Base Address). When you
first run OOIChem, you must enter these values in the “Configure Hardware” dialog box. (Remember
that Input/output settings are expressed in hexadecimal.)
tab and double-click on “Computer” at the top of the list of devices in
, find available settings -- numbers unassigned to hardware. Note these
and double-click on the
System
icon.
6
For most computers, the default settings on our A/D converters work well. In the picture at left on
page 6, it appears that the Printer occupies IRQ Setting 07, but for most computers, our A/D
converters can share the IRQ 07 setting with a printer and conflicts will not arise. All computers have
multiple Base Address (Input/output) settings from which to choose.
Windows NT Users:
Find Available Base Address and IRQ Settings
1. Go to
2. In the “Windows NT Diagnostics” dialog box, click on the
3. Select the
4. Select the
5. Note these available settings. When you first run OOIChem, you must enter these values in the
Start | Programs | Administrative Tools (Common) | Windows NT Diagnostics
Resources
IRQ
button. Find an available IRQ -- a number unassigned to a device.
I/O Port
numbers unassigned to a device. (T he number is in hexadecimal.)
“Configure Hardware” dialog box.
With Windows NT, devices cannot share IRQs; each device must be assigned a unique IRQ.
button. Find an available I/O Range (Base Address) -- a number or range of
tab.
.
1. Turn off the computer and remove the computer cover.
2. Ground yourself to the computer chassis or power supply.
3. Remove the PC2000 (spectrometer-A/D card combination) from its static-shielded bag.
4. If necessary, change the position of the switches on the A/D converter. Position the switches to match
the availab le settings you found in the previous section -- numbers not bei ng used by other hardware
devices. See
5. Find an open ISA-bus slot and remove the slot protector.
6. Insert the PC2000 into an available expansion slot on the motherboard connector by gently rocking the
card into the slot. Make sure the card is fully seated in the motherboard before screwing the tab on the
PC2000 to the computer. Do not bend the card or move it from side to side once it is seated in the slot.
7. If you have a CHEM2000, take your 400-
the SMA connector on your PC2000. If you have a CHEM2000-UV-VIS, take your 300-µ
solarization-resistant fiber that came with your system and screw one end into the SMA connector on
your PC2000. Reinstall the cover.
Appendix A
Install the PC2000
on page 34 for switch setting positions.
µ
m fiber that came with your system and screw one end into
7
m
Install OOIChem
The resolution of your computer's monitor must be 800 x 600 or better to use OOIChem software. Before
installing OO IChem, make sure that no other applic ations are running.
.
Setup.exe
.
1. Insert “Disk 1” into your floppy drive. (When prompted, install Disks 2 and 3.) Execute
2. At the “Welcome” dialog box, click
3. At the “Destination Location” dialog box, you can choose
Next>
Click
4. At the “Backup Replaced Files” dialog box, select either
you select Yes, you can choose
5. At the “Installation Complete” dialog box, choose
6. When the installation is complete,
.
Next>
.
Browse
Yes
or No. We recommend selecting Yes. If
Browse
to pick a destination directory. Click
Finish>
you must restart your computer
.
to pick a destination directory.
Next>
.
Run OOIChem
After you restart your computer, navigate to the OOIChem icon and select it. The first time you run
OOIChem after the installation, you must enter data into the Configure Hardware dialog box.
Configure Hardware
Configure Hardware
The
in this dialog box are usually set only once -- when OOIChem is first installed and the software first opens.
1. Under
2. Under
3. Under
Spectrometer Type
S2000/PC2000.
A/D Converter Type
ADC1000/PC2000.
Base Address
the dip switches on the A/D converter (and the
same availab le setting you found in your
computer).
dialog box opens automatically the first time you run the software. The parameters
, choose
, choose
, choose the same setting as
Remember that the Input/output Range (Base
Address) you selected was expressed in hexadecimal.
In this dialog box, the base address is given in
decimal, followed by the hexadecimal equivalent in
parenthesis. For example, “
base address as 768 decimal and 300 hexadecimal.
4. Under
5. Only these parameters apply to your system.
IRQ
, choose the same setting as the dip
switches on the A/D converter (and the same
available setting you found in your computer).
(Ignore the other settings; they apply to other
A/D converters.) Click OK. You can always change these settings once OOIChem is fully operational
by selecting
If you do not see the “Configure Hardware” screen, exit the software. Then select
type c:\windows\ooidrv.ini for Windows 95/98 systems or c:\winnt\ooidrv.ini
for Windows NT systems. Notepad will open. Edit this file for our device driver by finding the
“Initialized” entry and making sure this line reads Initialized=0. Save the OOIDRV.INI file and
exit Notepad. Restart OOIChem. You should now see the “Configure Hardware” dialog box.
Spectrometer | Hardware Configuration
768 (0x0300)
” gives the
from the menu.
Start
|
Run
, and
8
Using OOIChem Software
HEM OPERATING SOFTWARE
OOIC
signal processing, display, and measurement functions. The 32-bit software program operates with Windows
95/98 and Windows NT and performs basic absorbance, emission and kinetics functions, which are presented
in an easy-to-follow format that is ideal for undergraduate-level students and beginning spectroscopists.
OOIChem software provides users with five different modes of operation: Scope, Absorbance,
Transmission, Relative Irradiance, and Concentration. In addition, the software allows users to control data
acquisition features such as integration period, averaging, and boxcar smoothing -- directly from the
spectral graph display. Users can acquire data by taking manual single scans or by running continuous
scans, and add into the spectral window as many as 8 previously saved overlay spectra.
provides users with a real-time interface to a variety of acquisition,
Users can also perform kinetics
experiments, whic h all o ws user s
to monitor and report up to 4
single wavelengths or up to 2
calculated values from a
combination of wavelengths -for example, an absorbance value
of 400 minus an absorbance value
of 700. A kinetics chart displays
the time series. When the
experiment is complete, the data
can be exported to an ASCII file
for additional processing.
Another exciting feature of OOIChem
is that users can control the
parameters for all system functions
such as acquiring data, designing the
graph display, and configuring the
cursor. Additional features of
OOIChem include the ability to save
data as ASCII files and to store and
retrieve sample spectra.
OOIChem software will be updated
and improved continuously. To
obtain free upgrades, visit out web
www.oceanoptics.com/Software_Updates.asp
site at
the password found on the back of your Software and Resources Library CD.
If you find that you need more advanced spectrometer operating software, we have included, free of charge,
OOIBase32, a sophisticated, 32-bit, user-customizable advanced acquisition program. See the included
OOIBase32 manual for a list of functions and features.
. In order to download free upgrades, you will need
9
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