Oakton Instruments WD-35643-10 User manual

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Instruction Manual

DO 6+ Dissolved Oxygen/Temp

68X243634
Rev 0 Mar 09

Technol o

adeEas

Part of Thermo Fisher Scientific

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Instruction Manual DO 6+

Preface

This manual serves to explain the use of the DO 6+ hand held meter.
This manual functions in two ways: first as a step by step guide to help you

operate the meter; second, as a handy reference guide.
This manual is written to cover as many anticipated applications of the DO 6+

meter as possible. If there are doubts in the use of this meter, please do not
hesitate to contact the nearest Eutech Instruments Authorized Distributor.

Eutech Instruments will not accept any responsibility for damage or malfunction
to the meter caused by improper use of the instrument.

The information presented in this manual is subject to change without notice as
improvements are made, and does not represent a commitment on the part of
Eutech Instruments.

Copyright © 2009
Eutech Instrume
All rights reserved.

nts Pte Ltd

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Instruction Manual DO 6+

Table of Contents

1. INTRODUCTION...............................................................1

2. GETTING STARTED.........................................................2

2.1 Description of Keypad Functions...................................................................2

2.2 Description of LCD Annunciators...................................................................3

2.3 Inserting & removing the rubber armour / stand.............................................4

2.4 Inserting New Batteries.................................................................................4

2.5 Battery Replacement.....................................................................................5

2.6 Connecting the Electrode...............................................................................5

2.7 Electrode Information.....................................................................................6

2.8 Switching the Meter On .................................................................................7

2.9 Changing Mode.............................................................................................7

3. CALIBRATION..................................................................8

3.1 General Information.......................................................................................8

3.2 Temperature Calibration................................................................................10

3.3 % Saturation Calibration................................................................................11

3.3.1 100% Calibration...............................................................................11

3.3.2 0% Calibration...................................................................................12

3.4 mg/L or ppm Calibration ................................................................................13

4. MEASUREMENT ..............................................................14

4.1 Temperature Compensation..........................................................................14

4.2 Taking Measurements...................................................................................15

4.3 Pressure & Salinity Compensated Measurements.........................................16

4.4 Holding a Reading.........................................................................................16

5. SETUP...............................................................................17

5.1 Configuration Menu (COF.1)..........................................................................21

5.2 Automatic Temperature Compensation (A.ATC)............................................21

5.3 Selection of mg/L or ppm (b.DO)...................................................................22

5.4 Calibration Data (CAL.2)................................................................................22

5.5 Electrode Data (ELE.3)..................................................................................23

5.6 Automatic Shut Off (AtO.4)............................................................................24

5.7 Reset to Factory Default (rSt.5).....................................................................25

5.8 % Saturation Offset Adjustment (OFS.6) .......................................................26

5.9 Pressure & Salinity Adjustment......................................................................27

6. PROBE MAINTENANCE..................................................29

6.1 Cap and Electrolyte Replacement..................................................................30

6.2 Electrolyte Solution........................................................................................31

7. TROUBLESHOOTING......................................................31

8. ERROR MESSAGES

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........................................................32

Instruction Manual DO 6+

9. FACTORY DEFAULT SETTINGS ....................................33

10. SPECIFICATIONS ............................................................34

11. REPLACEMENTS AND ACCESSORIES.........................36

12. DISSOLVED OXYGEN THEORY .....................................37

Measurement Units.....................................................................................................38

What Is Being Measured?...........................................................................................38

Air Calibration.............................................................................................................39

Applications................................................................................................................39

13. WARRANTY......................................................................40

14.

RETURN OF ITEMS..........................................................40

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Instruction Manual DO 6+

1. INTRODUCTION

Thank you for purchasing the DO 6+. This microprocessor-based handheld
meter is economical and easy to use. It has a large custom LCD (Liquid Crystal
Display) for clear and easy reading.

The DO 6+ offers measurement of dissolved oxygen (DO) as percentage
saturation or concentration. Temperature measurement is also available in
degrees Celsius. The meter ensures accurate measurement of the dissolved
oxygen values through its temperature, barometric pressure and salinity
compensation features.

Meter kits include a dissolved oxygen probe, refill solution, a rubber armour /
stand, 4 alkaline “AAA” batteries, instruction manual, and warranty card. For
additional information, see Section 11 — REPLACEMENTS AND
ACCESSORIES.

Please read this manual thoroughly before operating your meter.

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Instruction Manual DO 6+

ST

2. GETTING STARTED

2.1 Description of Keypad Functions

Your meter has 6 keys on its splash-proof keypad.
Some buttons have multiple functions depending
on the mode of operation.

ON/OFF Powers meter on and off. Meter starts

CAL Enters into calibration mode. Pressing

MODE Selects desired measurement mode.

HOLD Freezes measured reading. Press again to resume live reading.
ENTER Confirms calibration value in calibration mode and confirm selections

up in the measurement mode that you
last switched off from.

while in calibration mode will abort
calibration without confirming value.

When pressed simultaneously with
ON/OFF, it will take you into the SETUP mode.
For more information see Section 5 — SETUP.

in SETUP mode.
Increment/decrement values during calibration mode or scroll through

SETUP menus. Set offset adjustments and configuration settings.

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r

2.2 Description of LCD Annunciators

Your meter has a large custom LCD that consists of 4-digit segments plus
annunciators for percentage saturation (%), concentration (mg/L or ppm) and
Temperature in degrees Celsius (T).

Other annunciators include “A” (when the ATC function is activated), “CAL”
(when meter is in calibration mode) and low battery condition.

Calibration Mode

Low Battery annunciato

Annunciator

CAL

14-segment Liquid Crystal Display

Active LCD dis play for DO 6+

Temperature Mod e

TA

mg/l
ppm

%

Percentage
(Percent age Saturation
Mode)

-3 -

A T C annunciator

milligram per litre
(Concentration Mode)

parts per million

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Instruction Manual DO 6+

2.3 Inserting & removing the rubber armour / stand

1. To remove meter from rubber a rmour, push out from the bottom edges of
meter until it is completely out of boot. Ensure that your electrode cables
are not connected. See Figure 1.

2. To insert meter into armour, slide in from the top of meter b efore pushing
the bottom edges of meter down to set it into position. Lift up the stand at
the back of meter for bench top applications if necessary. See Figure 2.

Ing New Batteries

Figure 1 Figure 2

2.4 Inserting New Batteries

The battery compartment is found at the back of instrument. To open the battery
compartment, push in the direction of arrow and lift up the cover. Note the
polarity of battery before inserting into position. After replacement, place cover
back and press down until it locks.

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2.5 Battery Replacement

A low battery annunciator in the LCD alerts you when battery power is running
low. Caution: Power off the meter before replacing battery.

%

Low Battery Condition

2.6 Connecting the Electrode

1) To connect the electrode, align the connector slots with the posts of

meter’s socket and rotate connector clockwise until it locks.

2) To remove, rotate the connector in anti-clockwise direction until it

unlocks, and slide the connector off the socket.

3) Insert the mini phono jack of temperature sensor into the socket on the

meter as shown below.

2.5 mm mini phono

BNC connector

for temperature

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2.7 Electrode Information

The DO 6+ includes a galvanic DO electrode
(93X233912) which doesn’t require warm-up. It
generates a millivolt signal proportional to the
amount of oxygen in the solution.

The electrode has a dual cable; a BNC connector
for DO measurement and a phono jack plug for
temperature. The sensor utilizes a cathode,
anode, and electrolyte that are separated from
the sample by an oxygen permeable membrane.
The membrane is pre-assembled for you and
fixed to a detachable cap. The pre-assembled
cap design allows simple replacement and fast
conditioning.

The probe is light weight and includes a built-in
temperature sensor. The epoxy body of the probe
is 12mm diameter while the detachable Noryl cap
is 16mm diameter. The compact sensing area
reduces air entrapment resulting in quick,
accurate, and stable readings.

The pre-assembled cap must be completely
submersed to obtain an accurate reading in
solutions. Provide simple stirring for best
results—ideally achieve a minimum water flow
rate of 2 inch/second on the membrane. The
probe is not recommended beyond 0 to 50 °C.

12 mm

Detachable
Pre-membraned Cap
(Probe Sensing Area )

Shaking will aid to remove bubbles if needed

16 mm

before taking a reading. When calibrating in air,
shake to remove water from the membrane.

The membrane is thin and can not be repaired if

Galvanic DO probe
(93X233912)

damaged. Use care to protect from scratches,
abrasion, or contact with solids. For best results
keep membrane clean by rinsing after daily use.
See Section 6 – PROBE MAINTENANCE.

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ON

2.8 Switching the Meter On

OFF

CAL 13 CAL 24

Press ON/OFF to power up your meter. Your meter will
cycle through various setup parameters when switched on.

1) All LCD segments will briefly illuminate.

2) The model [DO 6+] will display next along with mg/l,

ppm, and % annunciators.

3) Next, the meter revision number [r #.##] is shown

briefly before beginning measurement.

4) The meter begins with the measurement mode that

was in use when it was previously powered off%,
mg/l, ppm, or T.

TAvg

mg/l
NTU
ppm
%pH

mg/l
ppm

%

A

2.9 Changing Mode

%

Press MODE to switch between percentage saturation (%), concentration
(mg/L)(ppm), and temperature (T) measurement.

MODE

MODE

MODE

A

%

A

mg/l

TA

Automatic Temperature
Compensation

Percentage Saturation

Concentra t ion Mode

Temperature Mode

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Instruction Manual DO 6+

3. CALIBRATION

3.1 General Information

The DO 6+ has three measurement modes; DO as % saturation, DO as mg/L
(or ppm) concentration, and temperature.

Dissolved oxygen levels vary with temperature, barometric pressure, and
salinity, so calibration must be performed with consideration of these factors. It

is necessary to set the proper temperature, barometric pressure and
salinity values prior to performing any DO calibration or measurement.

See Section 3.2 — Temperature Calibration
See Section 5.2 — Automatic Temperature Compensation.
See Section 5.9 — Pressure & Salinity Adjustment.
The DO 6+ will accept two % saturation calibration points; 100% using

saturated air or air-saturated water, and 0% using zero oxygen solution. When
100% calibration is performed, the corresponding concentration is adjusted
simultaneously. Therefore, it is not necessary to calibrate the concentration
mode. If calibrating for 0% oxygen, note that the meter will take several minutes
to reach 0% saturation value and constant stirring is not required.

The following table lists calibration values in % saturation calibration mode with
two different barometric pressures. Note that the saturation value (92.1%) has
decreased due to the lower barometric pressure entered.

% Saturation (per

factory default value)

less than 10% 0% 0%

10.1% to 49.9% Err.1 (error 1) Err.1 (error 1)
50% to 200% 100% 92.1%

If calibration is attempted from 10.1% to 49.9%, the “Err.1” message is shown—
calibration is rejected and the display will return to measurement mode.

Calibration Value

(760mmHg)

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Calibration Value

(700mmHg)

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The DO 6+ will accept one calibration point in concentration mode. The
minimum value is 2 mg/L (ppm), and the calibration window is +/- 40% of the
factory default value.

Temperature and % saturation calibration should take place before attempting
to perform mg/L (ppm) concentration calibration.

Calibration of the concentration mode will only replace the previous
concentration calibration and does not affect the % saturation calibration.

To offset your % saturation reading to match another instrument, see Section

5.8 — % Saturation Offset Adjustment.
New calibration values will automatically override the existing data. Perform

daily calibration for best results. To completely recalibrate the meter and when
installing a replacement electrode, it is best to clear all calibration data. See
Section 5.7Reset to Factory Default.

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Instruction Manual DO 6+

3.2 Temperature Calibration

TTA

For best DO accuracy, ensure that the
temperature is accurate. The temperature
sensor of the DO 6+ probe has been
factory calibrated, however if it changes
over time or if the probe is replaced,
calibration may be necessary. To protect

CAL

CAL

A

from erroneous calibrations, the allowable
tolerance is limited to ±5°C adjustment of
the factory default value.

Most users utilize automatic temperature

CAL

Factory default
Temperature
Measurement

TA

compensation using the sensor that is built
into the probe. However, manual
temperature compensation can be used to
input a fixed, known temperature.

1) Press MODE to select temperature

HOLD

ENTER

mode. The display should show “T”
for temperature and “A” for automatic
temperature compensation.

2) Dip the probe into a solution with a
known, accurate temperature (i.e. a
temperature bath). Allow enough time
for the temperature reading to

“CO” wi ll displa y for

1.5 seconds

TA

stabilize.

3) Press CAL. The CAL indicator will
blink above the display. The
temperature value shown is the value based on the factory default.

4) Press Ÿ ź to adjust the reading to match the correct tempe rature value
(i.e. of the temperature bath).

5) Press HOLD/ENTER to confirm the calibration and return to measurement
mode.

Press CAL as needed to exit calibration without confirmation at any time.

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A

3.3 % Saturation Calibration

The DO6+ can be easily calibrated in air. For best DO accuracy, ensure that the
barometric pressure value is accurate.

The barometric pressure factory default is
760 mm Hg, which results in a theoretical
calibration value of 100% saturation in air.
If the barometric pressure setting has been
changed from 760 mm Hg, the meter will
automatically adjust to a new % saturation
calibration value instead of 100%. This new
value is correct for the adjusted barometric
pressure.

CAL

CAL

A

%

3.3.1 100% Calibration

1) Rinse the probe well with DI water or
rinse solution.

2) Press MODE to select % saturation.

3) Hold the probe in the air with the tip
facing downwards. Wait for the
reading to stabilize.

4) Press CAL. The CAL indicator and
intended percentage calibration point
(100%) display briefly, before CAL
flashes. The % value based on the
factory default calibration is shown.

5) Press HOLD/ENTER to confirm the
calibration. The meter displays “CO”,
automatically calibrates to 100.0% air
saturation, then returns to
measurement mode.

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CAL

HOLD
ENTER

%

Calibration point will be
displayed for 1.5 seconds

%

value with respect to the
factory default calibration
will be displayed

“CO” will dis played f or

1.5 seconds

A

%

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A

3.3.2 0% Calibration

1) Rinse the probe well with DI water or

A

rinse solution.

2) Press MODE to select % saturation.

3) Dip the probe into zero oxygen
solution. Wait for the reading to
stabilize.

CAL

CAL

%

4) Press CAL. The CAL indicator and
intended percentage calibration point
(0%) display briefly, before CAL
flashes. The % value based on the
factory default calibration is shown.

5) Press HOLD/ENTER to confirm the

CAL

%

Calibration point will be
displayed for 1.5 seconds

calibration. The meter displays “CO”,
automatically calibrates to 0.0%
saturation, then returns to
measurement mode.

HOLD

ENTER

“CO” will displayed for

1.5 seconds

%

value with respect to the
factory default calibration
will be displayed

A

%

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A

3.4 mg/L or ppm Calibration

Performing a 100% saturation calibration will

A

mg/l

simultaneously calibrate the corresponding
mg/L (ppm) concentration value. Therefore,
additional mg/L (ppm) calibration isn’t
required in most circumstances.

If desired, you can perform a calibration
adjustment in mg/L (ppm) without affecting

CAL

CAL

mg/l

your % saturation calibration value.

1) Perform 100% saturation calibration.
See Section 3.3.1 — 100% Calibration.

2) Rinse the probe well with DI water or
rinse solution.

CAL

value with respect to the
factory default calibration
will be displayed

mg/l

3) Dip the probe into a sample of known
oxygen concentration (i.e. determined
by titration or another instrument). Wait
for the reading to stabilize.

HOLD

ENTER

4) Press MODE to select the mg/L (ppm).

5) Press CAL. The CAL indicator and
current concentration display briefly,
before CAL flashes. The concentration
value based on the factory default
calibration is shown.

6) Press Ÿ ź to adjust the reading to

“CO” will display
for 1.5 seconds

A

mg/l

match the known oxygen concentration
value.

7) Press HOLD/ENTER to confirm the
calibration. The meter displays “CO”, automatically calibrates to the
entered value, then returns to measurement mode.

NOTE: The minimum calibration value is 2 mg/L. To prevent erroneous
calibrations, the calibration is limited to ±40% adjustment of the factory default
value.

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A

4. MEASUREMENT

The DO 6+ offers automatic or manual temperature compensation.
See Section 5.2 — Automatic Temperature Compensation.

A

%

utomati c Temperat u re

Compensation activated

4.1 Temperature Compensation

Insert the phono plug of the probe for Automatic Temperature Compensation
(ATC). The “A” annunciator will display normally when active, or blink if the
phono plug is disconnected.

Deactivate “A.ATC” for manual temperature
compensation. The “A” annunciator is not
displayed when the meter is in manual
temperature compensation mode.

For manual temperature compensation, manually
enter the desired process temperature into the
meter from 0 and 50 °C. Default is 25 °C.

1) Press MODE to select temperature.

2) Press CAL. The “CAL” indicator will start
blinking and the display will show the last
manually set temperature value.

3) Check the temperature of your sample using
an accurate reference thermometer. Wait for
the value to stabilize. Press Ÿ ź to manually
set the temperature value.

4) Press HOLD/ENTER to confirm the
calibration. The meter displays “CO”,
automatically sets the entered value, then
returns to measurement mode.

CAL

CAL

CAL

HOLD

ENTER

T

T

Last set manual
temperature setting

T

“CO” will display for 1.5
seconds

T

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4.2 Taking Measurements

Follow these general rules when taking measurements: keep the membrane
free from contact with solid objects, provide stirring of your solutionthis helps
to overcome the oxygen consumption of the probe and prevents air bubble
entrapment, do not strike against hard surfaces, and do not submerge the cable
for extended periods.

1) Rinse the probe well with DI water or
rinse solution.

2) Select the appropriate measurement
mode. Press MODE to toggle
between modes:

a. Percentage Saturation (%)
b.

Concentration (mg/L) or (ppm)

c. Temper

3) Dip the probe

4) Allow reading to stabilize and observe
reading.

To change the concentration units see

Section 5.3

—Selection of mg/L or ppm.

ature (T)

into the sample.

MODE

MODE

A

%

A

mg/l

TA

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4.3 Pressure & Salinity Compensated Measurements

During measurement the dissolved oxygen reading is automatically
compensated for salinity and pressure based on values entered in the setup
menu. For best DO accuracy, ensure pressure and salinity are adjusted
accordingly from the setup menu. The factory default values are 760 mm Hg
(101.3 kPa) barometric pressure (sea level) and 0.0 ppt salinity (no salinity).
See Section 5.9 — Pressure & Salinity Adjustment.

4.4 Holding a Reading

To hold the displayed reading momentarily, press HOLD/ENTER during any
measurement. The %, mg/L, ppm or T annunciator will blink, indicating that the
value is held.

A

%

Press HOLD/ENTER again to deactivate the HOLD function. The meter reverts
to the current active measurement and the annunciator will stop blinking.

If the auto-off feature is activated, the meter will turn off automatically after 20
minutes of non use The HOLD value is not retained when the meter is shut off
automatically or manually.

Annunciator Blinks when
meter is in “HOLD” mode

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5. SETUP

There are two setup menus. One menu is derived from the % saturation and
temperature
concentration

1) Press MODE to display % or temperature. Press ON/OFF to power off.

2) With the meter off, keep the MODE key pressed. Press and release

Press CAL one or more times as needed during setup mode to exit and return
to measurement mode at any time.

modes, the other menu is derived from the mg/L (or ppm)

mode.

OR
Press MODE to display mg/L (or ppm). Press ON/OFF to power off.

ON/OFF, then release MODE. The meter should display “SEt.P” after
ON/OFF is released and “COF.1” after MODE is released.

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Setup Menu

Configuration Menu

A.ATC Automatic Temperature Compensation
b.DO Select mg/L or ppm units for concentration mode

(available from concentration setup only)

View the latest calibration data according to the respective setup
menu.

View the electrode properties

FACT View the slope factor
OFS View the % saturation offset adjustment.

(available from % saturation concentration setup only)

HI.mV View the mV value at 100% saturation
LO.mV View the mV value at 0% saturation

Selection of automatic power-off (20 min from the last key press)

Reset Menu

A.CAL Calibration only reset to factory default
b.USR User settings reset to factory default

% saturation offset adjustment
(available from % saturation concentration setup only)

Dissolved Oxygen Parameters

A.HG Barometric pressure adjustment in mmHg
A.PA Barometric pressure adjustment in kilopascal
b.SAL Salinity adjustment

(available from concentration setup only)

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MODE

Switch off from % Saturation
or Temperature measurement
mode. Press and hold MODE

ON

key and then switch on.

OFF

SEt.P will display for
1 second

% or Temperature Setup Menu

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MODE

Switch off from Concentration
measurement mode. Press
and hold MODE key and then

ON

switch on.

OFF

SEt.P will display for
1 second

mg/L (ppm) Concentration Setup Menu

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5.1 Configuration Menu (COF.1)

5.2 Automatic Temperature Compensation (A.ATC)

Use this menu to change automatic temperature compensation (A) or units of
concentration (mg/L or ppm). ATC is recommended for most applications.

1) From “COF.1” of either setup menu, press
HOLD/ENTER to display “A.ATC”.

2) Press HOLD/ENTER to enter the selection
menu.

HOLD

3) Use Ÿź to select YES (activate ATC) or NO
(deactivate ATC and activate manual
temperature compensation).

4) Press HOLD/ENTER to confirm.

5) Press CAL to return to measurement mode.

ENTER

HOLD

ENTER

Selection of Automatic or

Manual Temperature

Compensation

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5.3 Selection of mg/L or ppm (b.DO)

1) From “COF.1” of the mg/L (ppm) setup
menu, press HOLD/ENTER three times to

mg/l

display “b.DO”.

2) Use Ÿź to select the desired units of
measurement.

3) Press HOLD/ENTER to confirm.

4) Press CAL to return to measurement mode.

ppm

5.4 Calibration Data (CAL.2)

To view the % saturation or mg/L (ppm) concentration calibration data, proceed
from the corresponding setup menu.

1) From the setup menu, press Ÿź to select
“CAL.2” for the most recent calibration data.

2) Press HOLD/ENTER to view calibration data.

3) Press HOLD/ENTER or CAL to exit.

4) Press CAL to return to measurement mode.

Note: “ -- -- -- -- ” indicates no calibration data exists
for the selected mode.

HOLD
ENTER

CAL

Display will show the latest % saturation
calibration if set up menu is selected from the
DO % Saturation Mode

CAL

%

mg/l

Display will show the latest mg/l or ppm
calibration if set up menu is selected from the
DO mg/l (ppm) Concentration Mode

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5.5 Electrode Data (ELE.3)

Use this menu to view electrode data for diagnostic purposes. Data includes;
slope factor (FACT), % saturation offset (OFS), 100% saturation mV value
(HI.mV), and 0% saturation mV value (LO.mV). To view electrode data for %
saturation or mg/L (ppm) modes, enter from the corresponding setup menu.

The electrode slope factor gives an indication of the probe’s efficiency. It is the
ratio of the actual mV produced by the probe to the theoretical mV value. The
ratio displays from 0.5 to 1.999.

The % saturation offset allows you to view the electrode offset adjustment made
in Section 5.8 — % Saturation Offset Adjustment.

1) From the setup menu, press Ÿź to select “ELE.3”.

2) Press HOLD/ENTER to enter the menu. “FACT” is momentarily shown
before displaying the slope factor.

3) Press HOLD/ENTER again. “OFS” is momentarily sho wn befo re displaying
% saturation offset. Note: this is not available from the mg/L (ppm)
concentration setup menu.

4) Press HOLD/ENTER again. “HI.mV” is momentarily shown before
displaying the electrodes mV output at 100%.

5) Press HOLD/ENTER again. “LO.mV” is momentarily shown before
displaying the electrodes mV output at 0%.

Press CAL to exit the setup mode and return to measurement mode at any
time.

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HOLD
ENTER

“FACT” displays for

1.5 seconds

Viewing of the offset
percentage adjustment will be
available only if set up menu is
selected from the DO %
Saturation or the Temperature
Mode. Otherwise, this menu
will be skipped.

HOLD
ENTER

HOLD
ENTER

“HI.mV” displays
for 1.5 seconds

“LO.mV” displays
for 1.5 seconds

HOLD

ENTER

“OFS” displays for

1.5 seconds

%

5.6 Automatic Shut Off (AtO.4)

Use this feature to conserve batteries. When active, the
meter will automatically shut off 20 minutes after the last
key press.

1) From the setup menu, press Ÿź to select “AtO.4”.

2) Press HOLD/ENTER to enter the menu.

3) Use Ÿź to select YES (activate automatic off) or
NO (activate automatic off).

4) Press HOLD/ENTER to confirm.

5) Press CAL to return to measurement mode.

HOLD

ENTER

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5.7 Reset to Factory Default (rSt.5)

Use this mode to reset the meter to factory default settings. There are two
levels of reset.

Calibration (A.CAL) resets calibration values only.
User (b.USR) resets all data, calibration, and other customized setup functions.

1) From the setup menu, press Ÿź to select
“rSt.5”.

2) Press HOLD/ENTER to enter the menu.

3) Press HOLD/ENTER to enter calibration reset
“A.CAL”.

4) Use Ÿź to select YES (reset calibration) or NO
(do not reset calibration).

5) Press HOLD/ENTER to confirm. If “YES”, the
meter returns to measurement mode after
resetting.
Note: Both % and concentration calibrations are
reset when from the % saturation setup.
However, when calibration reset occurs from
the concentration setup, only concentration is
reset.

6) If “NO”, the User Reset menu “b.USR” is
displayed.

7) Press HOLD/ENTER to enter the menu.

8) Use Ÿź to select YES (reset user) or NO (do not reset user).

9) Press HOLD/ENTER to confirm.

10) If “YES”, the meter returns to measurement mode after resetting.

11) Press CAL to return to measurement mode.

HOLD

ENTER

HOLD

ENTER

Calibration
Reset

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5.8 % Saturation Offset Adjustment (OFS.6)

Use this feature to offset the meter’s value
when cross referenced to another DO
meter. The DO 6+ allows +/- 10.0% offset
adjustment. View the offset value from the
Electrode Data menu “ELE.3”.

1) Using the % saturation mode of your
DO6+, observe the reading a sample
solution after it has stabilized.

2) Similarly, observe the reading of the
same sample using another DO meter
as a reference. The probe of the
reference meter should be immersed
in the same sample at the same depth.

3) Switch off the DO6+ and enter the %
saturation setup menu.

4) Press Ÿź to select “OFS.6”.

5) Press HOLD/ENTER to enter the
menu. The display will momentarily
show the measured reading based on
the last calibration before displaying
the last offset adjusted value.

6) Use Ÿź to enter the value of the
reference DO meter.

7) Press HOLD/ENTER to confirm.

8) Press CAL to return to measurement mode.

Note: User calibrations will reset the offset adjustment to 0.0 %.

HOLD
ENTER

%

Measured reading based on last
percentage saturation calibration
is displayed momentarily

%

Last offset adjusted
value will be displayed

%

HOLD

ENTER

“CO” will display for

1.5 seconds

Display will return to “OFS.6” subgroup menu

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5.9 Pressure & Salinity Adjustment

Use this menu to set barometric pressure and salinity values of the sample to
be measured. Use mmHg (A.HG) or kilopascal (A.PA) barometric pressure
units, and ppt salinity units (bSAL) from concentration setup menu. For best
accuracy, enter the actual salinity value if your samples are at least 0.1 ppt (100
ppm). Maximum adjustment is 50 ppt (50,000 ppm or 5% salt).

1)

Press MODE to
With the meter off, keep the MODE key pressed. Press and release

2)
ON/OFF, then release MODE. The meter should display “SEt.P” after
ON/OFF is released and “COF.1” after MODE is released.

3) Press Ÿź until the display shows “DPr.7”.

4) Press HOLD/ENTER. The display will show the current setting, either
“A.HG” (millimeters of mercury or mm Hg) or “A.PA”

5) Use Ÿź to select the desired barometric pressure units, then press
HOLD/ENTER to confirm.

6) Use Ÿź to set the actual pressure value and press HOLD/ENTER to
confirm. If values were changed, the confirmation indicator “CO” will
display briefly.

7)

Next, “b.SAL” (salinity adjustment) will display. “DPr.7” (main group

ill display if mg/L was not used in step 1). Note: if the concentration setup

w
was not used in step 1) the meter will return to “DPr.7” as salinity is only
accessable thru concentration setup.

8) Press CAL to return to measurement mode o r HOLD/ENTER for salinity
setting adjustment.

9) Use Ÿź to enter the salinity of your solution in parts per thousand (ppt).

10) Press HOLD/ENTER to confirm. If values were changed, a confirmation
indicator “CO” will display briefly.

11) Press CAL to return to measurement mode.

display mg/L concentration. Press ON/OFF to powe

(kilopascal or kPa).

r off.

menu)

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HOLD
ENTER

HOLD

ENTER

HOLD
ENTER

HOLD

ENTER

“CO” will display

Display will continue to
Salinity setting menu
“b.SAL” if Set Up menu is
entered from mg/l (ppm)
mode. Otherwise display will
return back to “DPr.7”

for 1.5 seconds

Pressure & Salinity Adjustment Sequences

HOLD

ENTER

HOLD

ENTER

“CO” will display

Display will continue to
Salinity setting menu
“b.SAL” if Set Up menu is
entered from mg/l (ppm)
mode. Otherwise display will
return back to “DPr.7”

for 1.5 seconds

- 28 -

HOLD

ENTER

“CO” wil l display for

1.5 seconds

Display will return back to “DPr.7”
subgroup menu

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Instruction Manual DO 6+

6. PROBE MAINTENANCE

The DO 6+ probe is a galvanic measuring element which produces an output
proportional to the oxygen present in the medium in which it is placed. The
galvanic probe design lets you take measurements immediately – without the
typical 15 minute wait of polargraphic dissolved oxygen probes.

The probe consists of two parts. The upper part consists of the anode, cathode,
and dual cable. The lower part consists of a pre-assembled cap, and electrolyte
solution.

Oxygen diffuses through the membrane onto the cathode, where it is
consumed. This process produces an electrical current which flows through the
cable to the meter. The electric current produced is proportional to the oxygen
that passes through the membrane and the layer of electrolyte. This makes it
possible to measure the partial pressure of oxygen in the sample at a given
temperature.

Since the DO in the sample is consumed by the cathode it is essential to have
flow past the membrane of the probe to prevent the occurrence of false
readings. The probe uses very little oxygen for its measurement. This enables it
to function correctly with liquid movement as low as 2 inch/sec across
membrane.

The permeability of the membrane to oxygen varies greatly with temperature.
Therefore compensation is needed for this variation. The DO 6+ probe comes
with built-in temperature compensation for the membrane variation.

Proper maintenance will help you maximize probe life and accurate readings.
Deposits on the membrane surface act as a barrier to oxygen diffusing through

the membrane, so clean the membrane to assure maximum reliability.
After each use, rinse the probe with clean water to avoid any hardening of

deposits. If growth develops on the probe, use a disinfecting chemical to clean.
NOTE: Although the membrane is strong and not easily damaged, wipe it gently

while cleaning it. If the membrane is punctured, damaged, or torn, the probe will
not function properly.

There are no special probe storage requirements.

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6.1 Cap and Electrolyte Replacement

Replacement of the pre-assembled cap is required only when you cannot
calibrate the probe, or if the membrane is damaged. Typical membrane
damages are punctures or wrinkles caused during measurements or cleaning.

For part numbers, see Section 11 – REPLACEMENTS AND ACCESSORIES.

1) Unscrew the cap counter clockwise from the probe sensing tip.

2) Rinse the probe under running water.

3) Mount the nozzle tip onto the syringe provided. Fill the syringe with the
refill solution through the tip of the plastic bottle.

4) Hold the probe upside down. Insert the nozzle tip into one of the 4 holes
surrounding the silver cathode. Inject the fill solution into the probe body
until solution leaks out from the fill hole (approximately 5 mL).

5) Replace pre-assembled cap by tightening clockwise until hand tightened.

6) Allow at least 1 hour for the electrode to equilibrate before usage.

1

4

2

5

3

6

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6.2 Electrolyte Solution

The electrolyte solution in your probe’s cap will deplete on usage and will need
to be replaced periodically. The replacement electrolyte solution included with
your probe comes premixed and ready to use. To order more electrolyte
solution, Section 11 – REPLACEMENTS AND ACCESSORIES.

7. TROUBLESHOOTING

Problem Cause Solution

No display
when
turned on

Unstable
readings

Slow
response

No
response
to key
press

a) Batteries not in place
b) Batteries not in correct

polarity (+ / – position)

c)

Weak batteries

a) Insufficient electrolyte in

probe

b) Air bubbles trapped around

the probe
c) Dirty or damaged probe
d) Probe not deep enough in

sample
e) External noise pickup or

induction caused by

nearby

electric motor
f) Broken probe
a) Dirty / Oily probe
b) Temperature
a) HOLD in use- indicated

by flashing display
b) Damaged pad
c) Internal program error

is cha

nging

a) Ensure batteries are in place and

making good contact

b) Re-insert batteries with correct

polarity

c) Replace batteries
a) Fill probe with electrolyte & / or

replace pre-assembled cap

b) Stir or tap probe to remove

bubbles

c) Clean the probe and re-

calibrate

d) Make sure sample entirely

covers the probe sensors

e) Move or switch off interfering

moto

r
f) Replace probe
a) Clean probe
b)

Allow temperature to stabilize
a) Press HOLD/ENTER
b) Return
c) Reset by reinserting batteries

to

dealer

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8. ERROR MESSAGES

LCD Display Indicates Cause Solution

“Err 1” in %
Saturation
Mode

“Err. 1” in mg/L
(ppm)
Concentration
Mode

“UR”/”OR” with
blinking “A”
annunciator in
Temperature
Mode

“----“with
blinking “A”
annunciator in
% Saturation
and
Concentration
Mode

Low battery Need new batteries or

% Saturation
calibration
error

Concentration
calibration
error

ATC probe
error,
Under Range,
Over Range

ATC probe
error

battery connection is
bad.

Calibration is attempted
when the factory
calibrated absolute
value is within

10.1% to 49.9%

Calibration is attempted
when the factory
calibrated absolute
value is below 2.00

ATC probe is
disconnected or broken
with the ATC feature
activated.
Temperature is out of
range

ATC probe is
disconnected or broken
with the ATC feature
activated.
Temperature is out of
range

Clean battery contacts.
Replace batteries,
noting polarity.

Check the value of the
calibration solution. If
zero calibration is done,
make sure the limit of
10% is not exceeded
Recondition your probe

Verify the solution is
above 2.00
Verify the temperature
and salinity settings
Recondition your probe

Connect the ATC plug
to the meter. Verify the
temperature accuracy.
Ensure probe is not
broken or punctured.

Connect the ATC plug
to the meter. Verify the
temperature accuracy.
Ensure probe is not
broken or punctured.

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9. FACTORY DEFAULT SETTINGS

MENU DESCRIPTION OPTIONS DEFAULT

COF.1 Configuration Setup Menu
A.ATC Automatic Temperature Compensation YES / NO YES
b.DO DO Concentration Units mg/L or ppm mg/L

CAL.2 Calibration Data
ELE.3
FACT Electrode Slope Factor View only 1.000

OFS
HI.mV View mV Value at 100% Saturation View only 50 mV

LO.mV View mV Value at 0% Saturation View only 0 mV
AtO.4 Automatic Power Off YES / NO YES
rSt.5

A.CAL Selection of Calibration Reset NO / YES NO
b.USR Selection of User Reset NO / YES NO
OFS.6 % Saturation Offset Adjustment +/- 10.0% 0.0%

DPr.7
A.HG Pressure Adjustment in mm Hg 500 – 1499 760

A.PA Pressure Adjustment in kPa 66.6 - 199.9 101.3
b.SAL Salinity Adjustment in ppt 0.0 - 50.0 0.0
Manual Temperature Compensation 0.0 - 50.0 °C 25.0°C

Electrode Data Menu

Viewing the % Saturation offset
adjustment.

Reset to Factory Default Menu

Dissolved Oxygen Parameters Menu

View only

View only 0.0%

_ _ _ _

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10. SPECIFICATIONS

% Saturation Mode
Range
Resolution
Relative accuracy

mg/L (ppm) Concentration Mode
Range
Resolution
Relative accuracy

Temperature
Range
Resolution
Relative accuracy

Salinity Correction
Range
Resolution
Method

Barometric Pressure Correction (mm Hg)
Range
Resolution
Method

Automatic Temperature Compensation 0.0 to 50.0°C
Manual Temperature Compensation 0.0 to 50.0°C
Probe (DO / Temp) Galvanic / Thermistor
Probe Diameter Body 12 mm, Cap 16 mm
Response Time 60 seconds to achieve 95% of the reading
% Saturation Calibration Points 100% in saturated air or air-saturated

0.00 – 200.0 %

0.1 %
± 1.5% of Full Scale

0.00 – 20.00 mg/L or ppm

0.01 mg/L; 0.01 ppm
± 1.5% of Full Scale

-5.0 – 105.0 °C (meter only)*

0.1 °C
± 0.5 °C

*Probe measures 0.0 – 50.0 °C

0.0 – 50.0 ppt

0.1 ppt
Automatic correction after manual input

500 to 1499 mm Hg or 66.6 to 199.9 kPA
1 mm Hg or 0.1 kPA
Automatic correction after manual input

water.
0% in zero oxygen solution

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% Saturation Calibration Limits Factory calibrated absolute value of 10.0%

Concentration Calibration Window +/- 40% from the factory default

Temperature Calibration Window +/- 5°C from factory default measurement
Offset Adjustments (% Saturation) +/- 10.0 of reading in Saturation mode
HOLD function Yes
Auto-Off function Selectable Auto Off function. (20 minutes

Display Custom Single 4 Digit LCD
Inputs BNC for DO & 2.5 mm Phono for

Operating Range 0 to 50 °C
Power Requirements 4 AAA-sized batteries (included)
Battery Life > 700 hours (Alkaline Batteries)
Dimensions Meter: 15.7 x 8.5 x 4.2 cm / 255 g

and below for 0% point & 50% to 200% for
100% point.

measurement value. Minimum reading
allowed is 2.00 mg/L (ppm).

after last press)

temperature

Probe: 115 mm x 12 mm (Dia), 3-ft cable
Membrane housing: 16 mm (Dia)

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11. REPLACEMENTS AND ACCESSORIES

Description

DO 6+ Dissolved Oxygen Meter in Hard Plastic Carry Case

DO 6+ Dissolved Oxygen Meter with 3 m Cable Electrode, (2)
Assembled Membrane Cap Housing, Refilling Electrolyte (10­mL) & Carrying Kit Set

DO 6+ Dissolved Oxygen Meter with 1 m Cable Electrode, 2
Assembled Membrane Cap Housing, Refilling Electrolyte (10­mL) & Carrying Kit Set

DO probe, 10-mL electrolyte and plastic syringe, 3 m Cable

DO probe, 10 mL electrolyte and plastic syringe, 1 m Cable

Hard Plastic Carry Case with (4) sample Bottles (60 mL)

Replacement Rubber Armour / Stand 15X410401
Assembled Membrane Cap 01X241608
Assembled Membrane Cap, 10-mL electrolyte, plastic syringe 01X241609
DO probe electrolyte refilling solution 01X211226 (50 mL)
Replacement Alkaline AAA batteries 60X023200

Order Code

Part Number

ECDO600PLUSK

01X370112

ECDO602PLUSK

01X370113

ECDO601PLUSK

01X370114

ECDO6HANDY3M

01X233916

ECDO6HANDY

01X233913

ECECODRYKIT

01X266903

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Instruction Manual DO 6+

12. DISSOLVED OXYGEN THEORY

Dissolved Oxygen (DO) refers to the volume of oxygen that is contained in
water. There are two main sources of DO in water; atmosphere and
photosynthesis. Waves and tumbling water mix air into the water where oxygen
readily dissolves until saturation occurs. Oxygen is also produced by aquatic
plants and algae during photosynthesis.

The amount of DO that can be held by water depends on 3 factors:

1) TEMPERATURE:
DO increases with decreasing temperatur
(colder

water holds more oxygen)

2) SALINITY:
DO increases with decreasing salinity
(freshwater holds more oxygen than saltwater does)

3) ATMOSPHERIC PRESSURE:
DO decreases with decreasing atmospheric pressure
(amount of DO absorbed in water decreases as altitude increases)

e

Solubil ity of oxy gen in water contact wi th water saturated air at

16

14

12

10

8

6

Solubility mg/L

4

2

0

0 5 10 15 20 25 30 35 40 45 50

standa rd atmospheric pressure

Temperature °C

DO Solubility in Water vs Temperature

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Measurement Units

One measure of DO in water is parts per million (ppm) which is the number of
oxygen molecules (O
% Saturation is another way to analyze DO levels. % Saturation is the
measured DO level divided by the greatest amount of oxygen that the water
could hold under various temperature and atmospheric pressure conditions
multiplied by 100.

) per million total molecules in a sample. Calculating the

2

What Is Being Measured?

DO probes respond to the partial pressure of oxygen in liquid or gas being
measured – they measure the “pressure” of oxygen rather than concentration.
All of the oxygen entering the probe is consumed at the cathode where it is
electrochemically reduced to hydroxyl ions producing an electrical current within
the probe:

O

+ 2 H2O + 4 e- Æ 4 OH –

2

Since all oxygen entering the probe is chemically consumed, the partial
pressure of oxygen in the electrolyte is zero. Therefore, a partial pressure
gradient exists across the membrane and the rate at which oxygen enters the
probe is a function of the partial pressure of oxygen in the gas or in liquid being
measured.

When a probe is placed in air saturated water, the current it produces will not be
affected by the temperature or salinity of the water. The DO concentration in
the water, however, will vary with temperature and salinity. Because it is
convenient to report DO concentration in mg/L or ppm, it is necessary to adjust
for temperature and salinity of the water to get correct readings in these units.

If DO were to be reported in terms of partial pressure or % Saturation, then
temperature and/or salinity compensation for oxygen solubility would not be
necessary. Most probes are temperature compensated – i.e. they convert the
“partial pressure measurement” to mg/L of DO at whatever temperature the
water happens to be at for a given salinity and barometric pressure.

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Air Calibration

Understanding the principle of air calibration is easy, once you know that it is
partial pressure that the probe is responding to. When the probe is in air, it is
measuring the partial pressure of oxygen in air. If water is air saturated, then
the partial pressure of oxygen in the water will be the same as it is in air.
Therefore, all you need to know is the temperature of the air in which the probe
is placed. By consulting solubility tables for oxygen at the particular barometric
pressure and salinity of the water being measured, the corresponding
concentration (mg/L or ppm) can be found for air saturated water at the air
calibration temperature, and the meter can be set accordingly. Because most
meters are temperature compensated, they will still give correct readings in
mg/L even though the actual water temperature may be different to the air
calibration temperature. Note: The closer the air calibration temperature is to
the water temperature, the more accurate the calibration.

Applications

Oxygen is essential for fish, invertebrate, plant, and aerobic bacteria respiration.
DO levels below 3 ppm are stressful to most aquatic organisms. Levels below 2
or 1 ppm will not support fish. Fish growth and activity usually require 5 to 6
ppm of DO, an important consideration for Aqua-culture industry.

Low DO indicates a demand on the oxygen of the system. Natural organic
material such as leaves accumulate in the stream and create an oxygen
demand as it is decomposed. Organic materials from human activities also
create an oxygen demand in the system. Micro-organisms consume oxygen as
they decompose sewage, urban and agricultural run-off, and discharge from
food-processing plants, meat-packing plants and diaries. There is an optimum
DO level for this process and if DO level falls too low, the micro-organisms die
and the decomposition ceases. If DO level is too high, more power is used than
necessary for aeration and the process becomes costly.

In boiler water application, presence of oxygen in the water will increase
corrosion and helps build up boiler scale that inhibits heat transfer. In such
instance it is critical to keep DO concentration to a minimum.

Some pollutants such as acid mine drainage produce direct chemical demands
on oxygen in the water. DO is consumed in the oxidation-reduction reactions of

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introduced chemical compounds such as nitrate (NO
sulfate (SO

2-

), and sulfite (SO

4

2-

) and ferrous (Fe

3

+

) and ferric (Fe

2

1-

) and ammonia (NH

3

+

) ions.

3

1+

),

4

These are important consideration for water and wastewater treatment industry.

13. WARRANTY

This meter is supplied with a three-year warranty, six-month warranty for
electrode against significant deviations in material and workmanship.

If repair or adjustment is necessary and has not been the result of abuse or
misuse within the designated period, please return – freight pre-paid – and
correction will be made without charge. Eutech Instruments will determine if the
product problem is due to deviations or customer misuse.

Out of warranty products will be repaired on a charged basis.

Exclusions

The warranty on your instrument shall not apply to defects resulting from:

x Improper or inadequate maintenance by customer
x Unauthorized modification or misu

Operation outside of the environment specifications of the products

x

se

14. RETURN OF ITEMS

Authorization must be obtained from our Customer Service Department or
authorized distributor before returning items for any reason. A “Return Goods
Authorization” (RGA) form is available through our Authorized Distributor.
Please include data regarding the reason the items are to be returned. For your
protection, items must be carefully packed to prevent damage in shipment and
insured against possible damage or loss. Eutech Instruments will not be
responsible for damage resulting from careless or insufficient packing. A
restocking charge will be made on all unauthorized returns.

Eutech Instruments Pte Ltd reserve the right to make improvements in
design, construction, and appearance of products without notice.

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