Novex EI8600, EI8700, EI8675, PowerEase 500 EI8600, PowerEase 500 EI8700 User Manual

USER GUIDE

PowerEase® 500 Power Supply

A programmable power supply for electrophoresis

Catalog Numbers EI8600, EI8700, and EI8675

Document Part Number A12162
Publication Number MAN0000738
Revision 9.0

For Research Use Only. Not for use in diagnostic procedures.

Table of Contents

Warning ................................................................................................................................................................... 4

Kit Contents ............................................................................................................................................................ 5

Product Specifications ........................................................................................................................................... 6

Front and Rear View of PowerEase® 500 ............................................................................................................ 7

Introduction .......................................................................................................................................................... 10

Description of PowerEase® 500 .......................................................................................................................... 10

Methods ....................................................................................................................... 12

Getting Started ..................................................................................................................................................... 12

Operational Modes .............................................................................................................................................. 13

Using PowerEase® 500 ......................................................................................................................................... 14

Printing .................................................................................................................................................................. 21

Custom Methods .................................................................................................................................................. 22

Troubleshooting ................................................................................................................................................... 24

Appendix ...................................................................................................................... 25

Instrument Symbols and Safety ......................................................................................................................... 25

Consignes De Securite ......................................................................................................................................... 26

Sicherheits Anweisungen ................................................................................................................................... 27

Run Conditions for Gels ..................................................................................................................................... 28

Repair, Maintenance, and Cleaning .................................................................................................................. 29

Background Information on Voltage, Current, and Resistance ..................................................................... 31

Accessory Products .............................................................................................................................................. 32

Technical Support ................................................................................................................................................ 33

3

Warning

Federal
Communications
Commission
Advisory

This equipment has been tested and found to comply with the limits for a Class
A digital device, pursuant to part 15 of the FCC rules. These limits are designed
to provide reasonable protection against harmful interference when the
equipment is operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if not installed and
used in accordance with the instruction manual, may cause harmful
interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference in which case the user
will be required to correct the interference at their expense.

Changes or modifications not expressly approved by the party responsible for
compliance could void the user’s authority to operate the equipment.

4

Kit Contents

Types of Kits

This manual is supplied with the following kits:

Kit Catalog No.

PowerEase® 500 Power Supply (100/120 Vac 50/60 Hz) EI8600

PowerEase® 500 Power Supply (220/240 Vac 50/60 Hz) EI8700

Kit Components

Note

PowerEase® 500 Pre-Cast Gel System
includes PowerEase

XCell

SureLock

®

500, Temperature Monitoring Probe,

™

Mini-Cell and XCell II™ Blot Module

EI8675

PowerEase® 500 Power Supply contains:

®

PowerEase

500 Power Supply 1 each

Instruction Manual 1 each

Extra Fuses 2 each

Power Cord (U.S., Canada, Europe, Taiwan and Japan) 1 each

Warranty Card 1 each

Temperature Probe (Optional) 1 each

®

See page 6 for specifications and detailed description of PowerEase

500 Power

Supply.

To ensure safe, reliable operation, always operate the PowerEase® 500 Power
Supply in accordance with the manufacturer’s instructions. Always wear
protective gloves and safety glasses when working in a laboratory environment.
See safety information on pages 25–27.

Warranty information is provided on page 33.

5

Product Specifications

Specifications

Input Power

100–120 VAC: 100–120V, 50/60 Hz, 1.2 A at maximum load

Fuse: One 1A/250V/AGC fast blow fuse

220–240 VAC: 220–240VAC, 50/60 Hz, 0.5 A at maximum load

Fuses: Two 1A/250V/5 × 20 mm fast blow fuses

Output Range 1–500 V (minimum step size 1 V)

1–500 mA (minimum step size 1 mA)

0.1–50 W (minimum step size 0.1 W)

Automatic crossover on reaching set limits.

Accuracy Voltage: ± 2% or ± 2 volts

Current: ± 2% or ± 2 mA

Wattage ± 2% or ± 0.2 watt

Drift < 1% in 8 hours after 30 minute warm-up
with constant supply voltage

Circuit Protection Open Circuit; Short Circuit; Thermal Protection
Over Voltage, Current, Wattage

Safety Load Detection

Thermal Shutoff (with optional temperature probe)

Isolated Floating Ground

Memory Control Non-volatile, up to 24 hours

Custom Methods Stored to RAM

Computer Interface RS232C

Display LCD, view area 114 x 64 mm

240 × 128 dots

Dimensions 16.5 cm (w) × 18 cm (h) × 20.5 cm (d)

Weight 2.9 kg (6.4 lbs)

Operating Ambient temperature 4–30°C, ≤80% relative
Environmental humidity, altitude < 2000 meters, indoor use only,
Conditions pollution degree 2

6

Front and Rear View of PowerEase® 500

Front View of
PowerEase

®

500

1

8

POWER

5

+

2

S



3

R

A

T

T

S

O

P

T

1. LCD Screen

2. Power Output Jacks, Positive (Red)

3. Power Output Jacks, Negative (Black)

4. Temperature Probe Jack

5. Functional Keys (Power, Start, Stop)

6. Directional Keys (Up, Down, Left, Right)

7. High Voltage LED

8. Power LED

P

SELECT

o

w

r

e

HIGH VOLTAGE

5

e

s

a

E

TEMP

7

0

0

6

4

Continued on next page

7

Front and Rear View of PowerEase® 500, Continued

Rear View of
PowerEase

®

500

Fan

Printer
Port

PRINTER

Power Entry Module
with On/Off
main power switch

RS232

RS232C
Communi­cation
Port

Continued on next page

8

Front and Rear View of PowerEase® 500, Continued

Key Pad

Power button secondary On/Off switch for the high voltage components, fan, and LCD

SELECT

screen.

Start button used to start a selected method program.

Stop button used to end the selected method and to backup to a previous screen.

Select button used to select a menu choice and to move forward to the next applicable
screen.

Left directional button for moving left around the menu.

Right directional button for moving right around the menu.

Up directional button for moving up around the menu and to adjust numerical and

character values (higher).

Down direction button for moving down around the menu and to adjust numerical and
character values (lower).

9

Introduction

Description of PowerEase

Product
Description

Features of
PowerEase

®

500

The PowerEase®500 Power Supply is a microprocessor-controlled power supply
for electrophoresis of pre-cast and hand-poured mini-gels. The power supply is
designed to simplify electrophoresis by combining high performance and
programming flexibility with ease of use when running mini-gel and blotting
applications.

®

The PowerEase
you need to think only about the electrical parameters and limits for a single gel.

This manual describes the setup and operation of PowerEase
including important information on safety and maintaining the unit.

The important features of PowerEase®500 Power Supply are:

• Large LCD display allows electrical parameters to be displayed in either

graphical or numerical formats

• Eight pre-programmed methods for running and blotting Novex

mini-gels

• Four custom methods for running your custom applications

• Simple three step operating procedure to start running or blotting Novex

pre-cast gels

• Microprocessor control provides highly accurate electrical outputs resulting

in exceptional blotting capability

• Parallel port for printing experimental results to most printers

• Optional Temperature Probe to continuously monitor the buffer temperature

and shut the unit down in the event of overheating

• Two sets of output jacks allows electrophoresis of multiple mini-cell units in

constant voltage, current, or watts

500 Power Supply is designed around the single gel concept:

®

500

®

500 Power Supply

®

pre-cast

®

10

Continued on next page

Description of PowerEase® 500, Continued

Flowchart

The following flowchart describes the various screens displayed on the

®

PowerEase

500 Power Supply and the keypad buttons (Start, Stop or Select)

used to navigate through the screens.

Power ON

Start-Up Screen

Start/Select

Gel Method Screen

Select

Gel Quantity Screen

Method Edit Screen

Method Run Large Display

Select

Method Run Large Display

StopStart

Previous Screen

Method Run Graphic Display

Start

Select, once Select, twice

Method Run Graphic Display

Stop

Previous Screen

Select

Method Run/Edit Screen

Select, onceSelect, twice

Method Run Large Display

11