This equipment must be operated as described in this manual.
If operational guidelines are not followed, equipment damage and
personal injury can occur. Please read entire User’s Guide
before attempting to use this unit.
Reproduction, adaptation, or translation without prior written permission from
New Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific Co., Inc. reserves the right to change
information in this document without notice. Updates to information in this
document reflect our commitment to continuing product development and
improvement.
Manual Conventions
#NOTE:
CAUTION!
Notes contain important and useful information
separate from text.
Caution messages appear before procedures
which, if not observed, could result in damage to
the equipment.
Warning messages alert you to specific
procedures or practices which, if not followed
correctly, could result in serious personal injury.
WARNING!
Bold Text in bold face type emphasizes key words or
The C-25 Classic Incubator and C-25KC Classic Refrigerated Incubator Shakers are large
capacity shakers utilizing an eccentric counter balanced drive to provide horizontal plane rotary
motion in a 1” (2.54 cm) circular orbit. A Proportional/Integral (PI) Microprocessor controller
with instantaneous digital feedback controls the speed over a range of 50-400 rpm.
The C-25 provides temperature control over a range of 7°C above ambient to 60°C, and the C-
25KC provides temperature control over a range of 15°C below ambient (minimum 4°C ) to
60°C. Ambient temperature is defined as the temperature within one meter of the shaker.
The shakers may be operated either continuously or in a timed mode via a programmable timer
for shaking periods of 0.1 hr. to 99.9 hrs.
For safe operation, both the C-25 and C-25KC are designed with a safety switch that
automatically stops the shaker mechanism when the lid is lifted.
In addition, the C-25 and C-25KC are equipped with visual and audible alarms that alert the user
to the following conditions:
• The end of a timed run
• Deviations of shaking speed
• Deviations of temperature setpoint
• Power failure
• Lid open
A wide variety of platforms can be used with the C-25 or C-25KC. Dedicated platforms are
available for a variety of flask sizes. Universal platforms, utility trays, utility carriers and test
tube racks are also available.
Speed:
Control Accuracy
Indication
Stroke
Temperature:
Control Accuracy
Indication
Ambient* Operating
Environment
Alarms
Timer
Automatic Restart
Drive Interrupt
Electrical Requirements
Platform
Overall Dimensions
Chamber Dimensions
Weight
*Ambient temperature is defined as the temperature within one meter of the shaker.
40-400 rpm
± 2 rpm
3 Digit LED, in 1 rpm increments
1” (2.54 cm)
7°C above ambient temperature* to 60°C
± 0.25°C
3 Digit LED, in 0.1°C increments
5 - 35°C, 20 to 90% relative humidity, non-condensing
Visible and audible warning indication when speed deviates
more than 5 rpm, and temperature more than 1°C from
setpoints, and when timer has expired.
0.1 hr to 99.9 hrs. Shuts off agitation at end of period. Can
be deactivated for continuous operation.
Automatic restart after power is restored. Setpoints and
operating status are retained in memory during power
interruption.
Automatic drive-interrupt when cover is opened.
110/120V AC 50/60 Hz, 1320 VA
220/240V AC 50/60 Hz, 1320 VA
30” X 18” (76 X 46 cm), Stainless Steel
44.3” W X 29” D X 33.4” H (113 X 74 X 85 cm)
23.5” W X 22” D X 19” clearance above platform (88 X 56 X
48 cm)
420 lbs (191 kg) Net, 450 lbs (204 kg) Gross
*Ambient temperature is defined as the temperature within one meter of the shaker.
40-400 rpm
± 2 rpm
3 Digit LED, in 1 rpm increments
1” (2.54 cm)
15°C below ambient temperature* (min. 4°C) to 60°C
± 0.25°C
3 Digit LED, in 0.1°C increments
5 - 35°C, 20 to 90% relative humidity, non-condensing
Visible and audible warning indication when speed
deviates more than 5 rpm, and temperature more than
1°C from setpoints, and when timer has expired.
0.1 hr to 99.9 hrs. Shuts off agitation at end of period.
Can be deactivated for continuous operation.
Automatic restart after power is restored. Setpoints
and operating status are retained in memory during
power interruption.
Automatic drive-interrupt when cover is opened.
R-134A
110/120V AC 50/60 Hz, 1980 VA
220/240V AC 50/60 Hz, 1980 VA
30” X 18” (76 X 46 cm), Stainless Steel
44.3” W X 29” D X 33.4” H (113 X 74 X 85 cm)
23.5” W X 22” D X 19” clearance above platform (88 X
56 X 48 cm)
450 lbs (204 kg) Net, 480 lbs (218 kg) Gross
After you receive your order from New Brunswick Scientific, inspect the boxes carefully
for any damage that may have occurred during shipping. Report any damage to the
carrier and to your local NBS Sales Order Department.
2.2 Unpacking
Save all packing materials and User’s Guide. If any part of your order was damaged
during shipping, missing pieces, or fails to operate properly, please fill out the Customer Satisfaction Form 6300 provided and return by fax.
WARNING!
Do not attempt to lift the C-25 or C-25KC by hand. Always use a
lifter or suitable equipment when raising the unit.
There are two small plastic straps holding the bearing housing in place during shipping.
Remove the straps from the bearing housing once the unit is unpacked and inspected.
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2.3 Inspection of Equipment
Verify against your NBS packing list that you have received the correct materials.
You should have received a Power Kit (power cord, fuse, hex wrench and key) with your
shaker. Make sure your electrical supply matches the electrical specification of the
power kit and the shaker (both of which should be the same).
It is essential that the instrument be situated in a area where there is sufficient space for
the shaker and platform to clear walls and obstructions during operation. The surface on
which the unit is placed must be smooth, level, and able to support the shaker under full
load operating conditions.
WARNING!
Do not attempt to lift the C-25 or C-25KC by hand. Always use
a lifter or suitable equipment when raising the unit.
The feet can be adjusted for necessary leveling. Loosen the locking nuts on the threaded
studs attached to the feet of the shaker. Retighten when you have achieved the correct
level for your shaker.
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3.2 Environment
The shaker is designed to operate optimally in the following ambient conditions:
• V(UP), W(DOWN)These keys are used to adjust the setpoint of a displayed parameter up or down. They also allow
the user to enter the
changes.
STATUS INDICATORS
Four status indicator lights are located to the left of the
•
MAINT Remains lit after 10,000 hours of use. Accumulated
running time is internally monitored and may be
displayed as a guideline.
•
SET Indicates that the shaker is in the SET MODE and
setpoints are being displayed and can be altered.
•
TIME Indicates that the timer is in operation. The shaker
can be programmed to run for a preset time from
0.1 hr. to 99.9 hrs. The timer can be disengaged
without stopping an ongoing run.
•
MUTE Indicates the status of the audible alarm. When
illuminated, the audible alarm is disabled.
FUNCTION INDICATORS
Four function indicator lights are located to the right of the
the current parameter being displayed.
•
RPM revolutions per minute
•
HOURS time remaining
•
°C interior chamber temperature
•
, not applicable
SET MODE for setpoint
LED DISPLAY. They are:
LED DISPLAY. They indicate
4.2 Platform Assemblies
The C-25 and C-25KC can be used with a wide variety of NBS 18 x 30 inch (46 x
76 cm)
A platform is a separate item that is required for operation. See Section 9,
Replacement Parts & Accessories, for details.
platforms, which will accept a variety of clamps for flasks test tubes, etc.
Page 24
23
5.1 Installation of Platform
A platform must be installed on the unit prior to use.
The unit is shipped with the four Allen (hexagonal, countersunk) head platform screws
installed in the subplatform of the bearing housing. These screws must be removed
before installing a platform. The Allen (“hex”) wrench is provided.
#NOTE:
There are two small plastic straps holding the bearing housing in place
during shipping. The straps must be removed from the unit
1. Using the 7/32-inch hex wrench provided, remove the four Allen head platform
screws from the sub-platform.
2. Place the platform on the
with the platform screw locations in the
3. Insert the four platform screws (Allen head) provided and tighten them with the 7/32inch hex wrench provided to secure platform.
Figure 2: Platform Screw Locations
55
SUBPLATFORM. Align the mounting holes of the platform
Flask clamps purchased for use with universal platforms (see Section 9.2)
require installation. Clamps are installed by securing the base of the clamp
to the platform with the correct type and number of screws. All clamps are
shipped complete with hardware.
Clamps for 2-, 2.8- and 4-liter flasks are shipped with an additional girdle to keep the
flasks in place. The girdle is an assembly of springs and sections of rubber tubing. One
girdle is already in place on the clamp, the other is packed separately. To install these
double girdle clamps:
1. Place the clamp on the platform, aligning its mounting holes with holes on the platform.
Secure the clamp in place using the flat Phillips head screws provided (#S2116-3051,
10-24 x 5/16-inch). Use Figure 3b to help you identify the proper screws, as three different types of screws are shipped with the clamps.
2. With the first girdle in place, as delivered, on the upper part of the clamp body (see Figure 3a), insert an empty flask into the clamp.
3. After making sure the sections of tubing are located between the clamp legs, roll the first
girdle down the legs of the clamp as far as it can go. The tubing sections will rest against
the platform, and the springs will be under the clamp base.
4. Place the second girdle around the upper portion of clamp body (just as the first girdle
was initially). Make sure that its spring sections rest against the clamp legs, while its
rubber tubing sections sit against the flask, in between the clamp legs.
The upper girdle secures the flask within the clamp, and the bottom girdle
keeps the flask from spinning.
NBS flask clamps are used on a variety of shaker platforms. Flat head screws of different
lengths and thread pitch are used to secure the clamp, and all types are provided when
you purchase clamps. To identify the proper screw for your shaker application by
reference to the head style, consult Table 1 below, find the proper screws and set the
others aside:
Table 1: Clamp Hardware Application Chart
No matter what size the clamp, use these screws to fasten them to your platform:
Description Part NumberQty. Application
10-24 x 5/16 (7.9 mm) flat
Phillips (+) head screw
S2116-3051 1 5/16" (7.9 mm) thick
aluminum, phenolic and
stainless steel
platforms.
5.3 Electrical Connections
Before making electrical connections, verify that the power source voltage matches the
voltage on the
OFF position. The ELECTRICAL SPECIFICATION PLATE is located on the side panel of
the unit near the
CONNECTOR
ELECTRICAL SPECIFICATION PLATE and the ON/OFF SWITCH is on the
ON/OFF SWITCH. Connect the POWER CORD to the POWER
and the other end to a suitable, grounded receptacle.
To initially start the shaker, close the lid and turn the
of shaker to the
the speed as it accelerates to the last entered setpoint. The shaking action may be stopped
or started by pressing the
ON position. If the shaker begins to operate, the LED DISPLAY will track
Figure 5: C-25 and C-25KC Front Panel
START/STOP KEY.
66
ON/OFF SWITCH on the front panel
O
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O
KEYPAD
ON/OFF
SWITCH
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#NOTE:
The shaker will not operate if the lid is open. This is indicated by the word
“lid” appearing in the LED DISPLAY.
3. Press either V or W KEY to enter SET MODE (the SET INDICATOR will illuminate).
4. Set the speed by using the V or
SELECT until the RPM INDICATOR is illuminated.
W KEY until the desired setpoint is displayed.
Holding the V or
W KEY will cause the setting to change more rapidly.
#NOTE:
The setpoint may be changed during a run without stopping the shaker by
following steps 2-4 above. During speed changes, a visual alarm (flashing
RPM INDICATOR) will flash and an audible alarm will sound until the speed
returns to within 5 rpm of the setpoint.
6.3 Checking a Setpoint
OFF, press the START/STOP KEY.
1. Press
2. Press either V or
SELECT until the desired indicator is illuminated.
CAUTION!
Holding the V or W key for more than 0.5 seconds causes the
speed setpoint to change. Should this occur, resetting will be
necessary.
6.4 Timed Functions
The shaker may be programmed to automatically stop after a preset time period of 0.1
hour - 99.9 hours. There must be power to the shaker in order to set the timer. However,
a timed run can be initiated while the unit is either shaking or stopped.
To set the timer:
1. Press the
2. Press either V or
3. While the
time (and start the run). The
duration of the run. At the end of the timed run the display will read
INDICATOR
SELECT KEY until the HRS INDICATOR is illuminated.
SET INDICATOR is illuminated, press the START/STOP KEY to program the
will flash and the audible alarm will “beep”.
W KEY to enter the SET MODE and display the current setpoint.
W KEY to enter the SET MODE and set between 0.1 - 99.9 hours.
To disable the visual alarm (flashing TIME INDICATOR), press the SELECT KEY and
change to any other function.
To cancel the timer without stopping the shaker:
Repeat steps 1 and 2. Then immediately press the
INDICATOR
will cease to flash and the display will read OFF.
START/STOP KEY. The TIME
6.5 Alarm Functions
The C-25 and C-25KC shakers have an audible alarm which is activated at predetermined
times. To deactivate the alarm:
1. Press
2. Simultaneously press the V and
SELECT until the HRS INDICATOR is illuminated.
W KEYS. The SET and MAINTINDICATORS will
flash.
3. While the
SET and MAINT INDICATORS are flashing press the START/STOP KEY.
The alarm may be reactivated by repeating steps 1-3. The
MUTE INDICATOR will
illuminate.
6.6 Temperature Setpoint
Press the
SELECT KEY until the function °C INDICATOR illuminates. The temperature can
be set from 7°C above (for C-25) and 15°C below (for C-25KC) the current ambient
temperature up to 60.0°C. Increasing or decreasing the setpoint is accomplished with the
V or
W KEY.
During operation, if the temperature of the chamber is more than 1.0°C higher or lower
than the temperature setpoint, an alarm is triggered. This alarm consists of a flashing
INDICATOR
and audible beep. The alarm will automatically deactivate as the unit
achieves the set temperature.
If desired, the temperature control system of the C-25KC may be shut off during set-up
for special investigations.
1. To deactivate the temperature control system press and hold the W
setpoint is at 4.0°C.
2. Holding the W
KEY, press the START / STOP KEY.
3. The temperature setpoint display shows
refrigeration system will be activated.
4. To reactivate the temperature controller, press the V
The control modules of the C-25 and C-25KC shaker totalize the time the shaker has
been “
ON” to track hours of usage. To display the accumulated running time:
1. Press
SELECT until the HRS INDICATOR is illuminated.
2. Simultaneously press the V and W
The
SET and MAINT INDICATORS will flash and the accumulated running time will be
displayed in hundreds of hours (i.e., “02” equals 200 hours; “102” equals 10,200 hours).
This display will continue for 10 seconds and then default to the previous mode readout.
6.8 Maint Indicator
After 10,000 hours of operation, the
maintenance is recommended at this point.
To deactivate the
MAINT INDICATOR:
1. Press
SELECT until the HRS INDICATOR is illuminated.
2. Simultaneously press the V and W
3. Press the W
KEY.
KEYS.
MAINT INDICATOR will illuminate. Preventive
KEYS.
6.9 Power Failure
In the event of a power failure, the C-25 and C-25KC shakers are equipped with an
automatic restart function.
If the shaker was in operation prior to the power interruption, the shaker will begin to
operate at its last entered setpoint. The
failure has occurred. Press any key to cease the flashing in the display.
Always turn off the shaker and disconnect the power cord from
the power supply BEFORE performing any maintenance on the
unit.
7.1 Cleaning External Surfaces
The unit may be cleaned using a damp cloth or any standard, household or laboratory
cleaner to wipe down its outer surfaces. Do not use abrasive or corrosive compounds to
clean this instrument, as they may damage the unit and void the warranty.
7.2 Fuse Replacement
The C-25 and C-25KC require two electrical fuses. They are housed in the fuse holders
located on rear panel of the unit near the
repeated below):
If any problems occur with your shaker, do not attempt to perform any service on the unit
other than specified in this manual. Unauthorized servicing may void the warranty. Please
contact your local NBS Sales Order Department
In any correspondence with NBS, please refer to the Model Number and Serial Number of your
unit. This information is on the
of the unit near the
ON/OFF SWITCH.
ELECTRICAL SPECIFICATION PLATE, located on the side panel
When ordering replacement or accessory parts, or requesting service information, please provide
the Model Number and Serial Number of your shaker. This information is on the
SPECIFICATION PLATE
9.1 Replacement Parts
V-Belt* 1 R-334
Housing Extractor Plate* 1 M1191-9300
3/8-16 NC x 1 ¼” long flat head Allen screw* 3 S1127-3206
Fuse, Slo Blo® 1.6 A, 250 V 1 P0380-3532
Fuse, Slo Blo® 0.16 A, 250 V 1 P0380-3710
*needed for belt replacement
, located on the side panel of the unit.
Table 2: Service Parts
Part Description Quantity
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9.2 Accessories
Platform Description Capacity NBS Part Number
See Table 5 on the following page M1250-9920
50 ml Erlenmeyer Flasks 108 M1191-9908
125 ml Erlenmeyer Flasks 60 M1191-9909
250 ml Erlenmeyer Flasks 40 M1191-9910
500 ml Erlenmeyer Flasks 24 M1191-9911
1 L Erlenmeyer Flasks 15 AG-1
2 L Erlenmeyer Flasks 12 AG-2
2.8 L Fernbach Flasks 6 AG-28
4 L Erlenmeyer Flasks 6 AG-4
6 L Erlenmeyer Flasks 4 AG-6
50 ml Erlenmeyer Flasks 92
125 ml Erlenmeyer Flasks 39
250 ml Erlenmeyer Flasks 30
500 ml Erlenmeyer Flasks 18
1 L Erlenmeyer Flasks 12
2 L Erlenmeyer Flasks 8
2.8 L Fernbach Flasks 6
4 L Erlenmeyer Flasks 6
6 L Erlenmeyer Flasks 4
Table 5: Carriers & Test Tube Racks
Accessory Description NBS Part Number
Utility Tray with rubber mat for shaking 96 well plates, petri
dishes and other labware at low speeds.
Angled Test Tube Rack Holder for user-supplied test tube
racks that are 4-5 inches (10-13 mm) wide and up to 15 inches
(38 mm) long. Capacity: 4 racks/platform.
Angled Test Tube Rack Spacer for use with TTR-210 to
accommodate test tube racks that are less than 5 inches
(13 mm) wide and up to 15 inches (38 mm) long.
Microtiter Plate Carrier, capacity up to 4 microtiter plates TTR-221*
80-tube (8-11mm ∅) Adjustable Angle Test Tube Rack M1289-0100a
60-tube (12-15mm ∅) Adjustable Angle Test Tube Rack M1289-0200a
42-tube (15-18mm ∅) Adjustable Angle Test Tube Rack M1289-0300a
30-tube (18-21mm ∅) Adjustable Angle Test Tube Rack M1289-0400a
22-tube (22-26mm ∅) Adjustable Angle Test Tube Rack M1289-0500a
20-tube (26-30mm ∅) Adjustable Angle Test Tube Rack M1289-0600a
Microplate Holder, stack 3 deep-well or 9 standard microplates M1289-0700
*Universal Platform Required
a Platform capacity is 7 racks
Platform capacity is 16 racks