Nanoentek SCC2 User Manual

ADAM

SCC

Automated Somatic Cell Counter

Instruction Manual

2

Copyright NanoEnTek, Inc. All rights reserved.

All the materials in this user manual are protected by Korean and international copyright laws. They cannot be
reproduced, translated, published or distributed without the permission of the copyright owner.

ADAM-SCC2 Instruction Manual

Website : www.nanoentek.com
E-mail : [e-mail]

Manufactured by

NanoEnTek, Inc.

851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel. [phone]
Fax. [phone]

NanoEnTek America, Inc.

240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA
Tel. [phone]
Fax. [phone]

The information in this manual is described as accurately as possible.
Firmware and software changes and updates may change without prior consent or notication.

Copyright © 2019 by NanoEnTek Inc.

All rights reserved. Published in Korea.

Documentation: NESMU-ASC2-001E

Revision history: V.0.0 AUG 2019

V.1.0 DEC 2019

Table of contents

Introduction

General description

Technology

Somatic Cell Count Kit

Product Contents

ADAM-SCC2

SOMA Chip kit

Upon receiving the instrument

Product Description

Front view of ADAM-SCC2

Rear view of ADAM-SCC2

Getting Started

Environmental requirements

Power on and initial display

Error messages during botting

Count setting

General Operation

Introduction

Sample preparation

Counting cell

Setting

2

3

4

Setting

Wi

Update

22

23

23

Power o

5

5

5

6

7

8

8

9

10

11

12

12

Lock

Power o

Maintenance and cleaning

Trouble shooting

Warranty

Technical specications

Product list

Safety precautions

Safety symbols

Warnings

Technical Support

24

24

25

26

27

28

29

30

31

32

33

Measure

Run sample

Result analysis

Result analysis -Error code

Data

Data list

Edit

Image

Mail

15

16

17

18

19

20

21

Introduction

General
Description

ADAM-SCC2 is an automated somatic cell counter integrated with uores­cence optic and image analysis software. ADAM-SCC2 counts the number
of somatic cells to determine the quality of raw milk with unique image
cytometer technology. ADAM-SCC2 can be used as an ideal equipment in
the research eld, dairy farms, milk manufacturing facilities, and veterinary
institutes working with somatic cell analysis. It will be helpful to manage
the milk quality to provide the best grade of milk.

2

Introduction

Technology

High levels of somatic cells cause disagreeable taste and reduce storage
life in dairy products. These somatic cell counts (SCCs) are accepted as an
international standard for the measurement of milk quality. For this reason,
somatic cell counts are readily available to a dairy farmer in most countries.

Standard reference method for enumeration of somatic cells in raw milk is
the direct microscopic somatic cell count (DMSCC). However, it needs the
training and skill of analysts for accuracy, precision, and reproducibility of
this method. ADAM-SCC2 is fully compatible with the DMSCC. It is com­posed of the disposable plastic microchips and staining solutions, a uo­rescence microscopic optics equipped with a CMOS camera, and an image
analysis system. It utilizes the capillary ow of the microuidic chamber
by the surface modication of hydrophilicity. Microuidic technology of
disposable microchips provides low reagent consumption and combining
with the ready-to-use reagent makes daily work easy.

ADAM-SCC2 is not only easy to use but oers the same repeatability and
accuracy as the conventional expensive device.

Excitation lter

Green LED

SOMA chip

Objective Lens

Dichroic Filter

Lenses

Mirror

CMOS Camera

Emission Filter

ADAM-SCC2, Automated Somatic Cell Counter 3

Introduction

Somatic Cell
Count Kit

Somatic Cell Count Kit of ADAM-SCC2 is composed of Propidium Iodide (PI)
for counting somatic cells. It can be used without diluting raw milk.

6

Measuring range of cell density is 0.05 ~ 1.15 x 10

Each SCC-Solution has 25 mL reagent of somatic stain solution. Simply add
100 μL volume of the raw milk sample and 100 μL stain solution in the

1.5 mL tube. Once the experiment is complete the results can be printed
through the optional thermal print. Printed number indicates cell concen­tration ( x 1000/mL) in each channel.

ㆍSOMA Chip 2X : Load 23 μL/Channel, 2 test/Chip
ㆍSOMA Chip 4X : Load 13 μL/Channel, 4 test/Chip

Store kit box upright and at room temperature. Expiration date of stain so­lution is written on the bottom of the kit box (yy-mm-dd). Be sure to check
the expiration date before using. Follow the exact steps detailed in the
Instructions for Use section.

cells/mL.

4

Product Contents

Introduction

ADAM-SCC2

SOMA Chip kit

The contents of the ADAM-SCC2 are listed below:

Item Quantity

Main device 1

Instruction manual 1

USB hub 1

Wi dongle 1

Power cord 1

Adapter 1

SOMA Chip Kit 1

Calibration Bead 1

Barcode scanner 1

The contents of the ADAM-SCC2’s Somatic Cell Count Kit are listed below:

Item SOMA Chip 2x Kit

Disposable Chip

SCC-Solution 25 mL x 1ea 25 mL x 2ea

Available test Q’ty

(Cat. No: CRS-K01)

50pcs (2 channel) 100pcs (4 channel)

100 test/kit 400 test/kit

SOMA Chip 4x Kit

(Cat. No: CRS-K02)

Upon receiving
the instrument

• Examine the instrument carefully for any damage incurred during transit.

• Ensure that all parts of the instrument including accessories listed above

are included with the product.

• Any damage claims must be led with the carrier.

• The warranty does not cover in-transit damage.

• Upon receipt, store SOMA Chip at room temperature.

• SCC-Solution should be stored at 2~8℃.

ADAM-SCC2, Automated Somatic Cell Counter 5

Introduction

Product Description

Front view of
ADAM-SCC2

The front view showing various parts of the ADAM-SCC2 is shown below:

Door

①

③

LCD

Power

②

④

⑤

⑥

Control buttons Description

① Door Slide holder is inserted and ejected.

② Power

③ LCD

④ START

⑤ LOCK

⑥ EJECT Ejects the slide holder from ADAM-SCC2. Functions as unload.

6

Power on / o.

Display processes and results.

Performs all procedures of automatic counting.

Protects the alignment of stage from external shock when
ADAM-SCC2 is being moved.

Lock ADAM-SCC2 before turning it o or moving it.

Introduction

Product Description

Rear view of
ADAM-SCC2

The rear view showing various parts of the ADAM-SCC2.

USB Port

①

Port Description

① USB Port

③ Power Plug

PC Port③Power plug

②

Port for software update and save the data.

Connects ADAM-SCC2 power cord to wall outlet.

CAUTION

!

Do not use the PC port. This port does not recognize USB.

②

ADAM-SCC2, Automated Somatic Cell Counter 7

Introduction

Getting Started

Environmental
requirements

CAUTION

!

At low temperature (≤10 °C), allow the device to warm up for 10 minutes at
ambient temperature before use.

To ensure correct operation and stable performance,
install the ADAM-SCC2 in a location which meets the following conditions:

1. Use at room temperature between 20 and 35 °C.

• Not recommended for cold room use (≤ 4 °C).

2. Do not expose the device to direct sunlight.

3. Do not subject the device to direct or continuous vibration.

4. Do not subject the device to intense magnetic or electromagnetic elds.

5. Do not install the device in high-humidity environment.

6. Location of device should be free from corrosive gases or other corrosive
substances.

7. Ensure minimal contact with dust or other airborne particles.

8. Allow a 10 cm (4 inches) minimum space around the device for proper
airow.

9. Do not place any objects on the device.

Power on and
Initial Display

8

1. Check the connection of ADAM-SCC2 and power cord.

2. Press the power button for 2~3 seconds.

If you get an error message,
please contact your local distributor or [e-mail].

If booting is successful and no errors are detected,
the home screens will be displayed as below.

CAUTION

!

•Do not tilt the device too much in the forward when connecting the power cord.

•Do not move the device after connecting power cord.

- When you connect the power cord to ADAM-SCC2 even without power on the device,
it will go through self diagnostic tests.