Nanoentek SCC2 User Manual

ADAM

SCC

Automated Somatic Cell Counter

Instruction Manual

2

Copyright NanoEnTek, Inc. All rights reserved.

All the materials in this user manual are protected by Korean and international copyright laws. They cannot be
reproduced, translated, published or distributed without the permission of the copyright owner.

ADAM-SCC2 Instruction Manual

Website : www.nanoentek.com
E-mail : [e-mail]

Manufactured by

NanoEnTek, Inc.

851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel. [phone]
Fax. [phone]

NanoEnTek America, Inc.

240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA
Tel. [phone]
Fax. [phone]

The information in this manual is described as accurately as possible.
Firmware and software changes and updates may change without prior consent or notication.

Copyright © 2019 by NanoEnTek Inc.

All rights reserved. Published in Korea.

Documentation: NESMU-ASC2-001E

Revision history: V.0.0 AUG 2019

V.1.0 DEC 2019

Table of contents

Introduction

General description

Technology

Somatic Cell Count Kit

Product Contents

ADAM-SCC2

SOMA Chip kit

Upon receiving the instrument

Product Description

Front view of ADAM-SCC2

Rear view of ADAM-SCC2

Getting Started

Environmental requirements

Power on and initial display

Error messages during botting

Count setting

General Operation

Introduction

Sample preparation

Counting cell

Setting

2

3

4

Setting

Wi

Update

22

23

23

Power o

5

5

5

6

7

8

8

9

10

11

12

12

Lock

Power o

Maintenance and cleaning

Trouble shooting

Warranty

Technical specications

Product list

Safety precautions

Safety symbols

Warnings

Technical Support

24

24

25

26

27

28

29

30

31

32

33

Measure

Run sample

Result analysis

Result analysis -Error code

Data

Data list

Edit

Image

Mail

15

16

17

18

19

20

21

Introduction

General
Description

ADAM-SCC2 is an automated somatic cell counter integrated with uores­cence optic and image analysis software. ADAM-SCC2 counts the number
of somatic cells to determine the quality of raw milk with unique image
cytometer technology. ADAM-SCC2 can be used as an ideal equipment in
the research eld, dairy farms, milk manufacturing facilities, and veterinary
institutes working with somatic cell analysis. It will be helpful to manage
the milk quality to provide the best grade of milk.

2

Introduction

Technology

High levels of somatic cells cause disagreeable taste and reduce storage
life in dairy products. These somatic cell counts (SCCs) are accepted as an
international standard for the measurement of milk quality. For this reason,
somatic cell counts are readily available to a dairy farmer in most countries.

Standard reference method for enumeration of somatic cells in raw milk is
the direct microscopic somatic cell count (DMSCC). However, it needs the
training and skill of analysts for accuracy, precision, and reproducibility of
this method. ADAM-SCC2 is fully compatible with the DMSCC. It is com­posed of the disposable plastic microchips and staining solutions, a uo­rescence microscopic optics equipped with a CMOS camera, and an image
analysis system. It utilizes the capillary ow of the microuidic chamber
by the surface modication of hydrophilicity. Microuidic technology of
disposable microchips provides low reagent consumption and combining
with the ready-to-use reagent makes daily work easy.

ADAM-SCC2 is not only easy to use but oers the same repeatability and
accuracy as the conventional expensive device.

Excitation lter

Green LED

SOMA chip

Objective Lens

Dichroic Filter

Lenses

Mirror

CMOS Camera

Emission Filter

ADAM-SCC2, Automated Somatic Cell Counter 3

Introduction

Somatic Cell
Count Kit

Somatic Cell Count Kit of ADAM-SCC2 is composed of Propidium Iodide (PI)
for counting somatic cells. It can be used without diluting raw milk.

6

Measuring range of cell density is 0.05 ~ 1.15 x 10

Each SCC-Solution has 25 mL reagent of somatic stain solution. Simply add
100 μL volume of the raw milk sample and 100 μL stain solution in the

1.5 mL tube. Once the experiment is complete the results can be printed
through the optional thermal print. Printed number indicates cell concen­tration ( x 1000/mL) in each channel.

ㆍSOMA Chip 2X : Load 23 μL/Channel, 2 test/Chip
ㆍSOMA Chip 4X : Load 13 μL/Channel, 4 test/Chip

Store kit box upright and at room temperature. Expiration date of stain so­lution is written on the bottom of the kit box (yy-mm-dd). Be sure to check
the expiration date before using. Follow the exact steps detailed in the
Instructions for Use section.

cells/mL.

4

Product Contents

Introduction

ADAM-SCC2

SOMA Chip kit

The contents of the ADAM-SCC2 are listed below:

Item Quantity

Main device 1

Instruction manual 1

USB hub 1

Wi dongle 1

Power cord 1

Adapter 1

SOMA Chip Kit 1

Calibration Bead 1

Barcode scanner 1

The contents of the ADAM-SCC2’s Somatic Cell Count Kit are listed below:

Item SOMA Chip 2x Kit

Disposable Chip

SCC-Solution 25 mL x 1ea 25 mL x 2ea

Available test Q’ty

(Cat. No: CRS-K01)

50pcs (2 channel) 100pcs (4 channel)

100 test/kit 400 test/kit

SOMA Chip 4x Kit

(Cat. No: CRS-K02)

Upon receiving
the instrument

• Examine the instrument carefully for any damage incurred during transit.

• Ensure that all parts of the instrument including accessories listed above

are included with the product.

• Any damage claims must be led with the carrier.

• The warranty does not cover in-transit damage.

• Upon receipt, store SOMA Chip at room temperature.

• SCC-Solution should be stored at 2~8℃.

ADAM-SCC2, Automated Somatic Cell Counter 5

Introduction

Product Description

Front view of
ADAM-SCC2

The front view showing various parts of the ADAM-SCC2 is shown below:

Door

①

③

LCD

Power

②

④

⑤

⑥

Control buttons Description

① Door Slide holder is inserted and ejected.

② Power

③ LCD

④ START

⑤ LOCK

⑥ EJECT Ejects the slide holder from ADAM-SCC2. Functions as unload.

6

Power on / o.

Display processes and results.

Performs all procedures of automatic counting.

Protects the alignment of stage from external shock when
ADAM-SCC2 is being moved.

Lock ADAM-SCC2 before turning it o or moving it.

Introduction

Product Description

Rear view of
ADAM-SCC2

The rear view showing various parts of the ADAM-SCC2.

USB Port

①

Port Description

① USB Port

③ Power Plug

PC Port③Power plug

②

Port for software update and save the data.

Connects ADAM-SCC2 power cord to wall outlet.

CAUTION

!

Do not use the PC port. This port does not recognize USB.

②

ADAM-SCC2, Automated Somatic Cell Counter 7

Introduction

Getting Started

Environmental
requirements

CAUTION

!

At low temperature (≤10 °C), allow the device to warm up for 10 minutes at
ambient temperature before use.

To ensure correct operation and stable performance,
install the ADAM-SCC2 in a location which meets the following conditions:

1. Use at room temperature between 20 and 35 °C.

• Not recommended for cold room use (≤ 4 °C).

2. Do not expose the device to direct sunlight.

3. Do not subject the device to direct or continuous vibration.

4. Do not subject the device to intense magnetic or electromagnetic elds.

5. Do not install the device in high-humidity environment.

6. Location of device should be free from corrosive gases or other corrosive
substances.

7. Ensure minimal contact with dust or other airborne particles.

8. Allow a 10 cm (4 inches) minimum space around the device for proper
airow.

9. Do not place any objects on the device.

Power on and
Initial Display

8

1. Check the connection of ADAM-SCC2 and power cord.

2. Press the power button for 2~3 seconds.

If you get an error message,
please contact your local distributor or [e-mail].

If booting is successful and no errors are detected,
the home screens will be displayed as below.

CAUTION

!

•Do not tilt the device too much in the forward when connecting the power cord.

•Do not move the device after connecting power cord.

- When you connect the power cord to ADAM-SCC2 even without power on the device,
it will go through self diagnostic tests.

Introduction

Getting Started

Error Messages
during booting

[System State]

It appears when booting is not working properly.
Turn o main power and restart device.

If this message still appears after restarting,
contact your local distributor or [e-mail].

Error code Cause

0x00000C00 Failure of X-axis sensor

0x00007000 Failure of Y-axis sensor

0x00008000 Failure of Z-axis sensor

0x06000000 Failure of Locking module sensor

ADAM-SCC2, Automated Somatic Cell Counter 9

IntroductionGetting Started

5

0

Count setting

Set the conditions in the ‘Setting’ tap before counting.

[SOMA Chip]

Set the SOMA Chip according to you are using.

Somachip

Somachip

T1 N1

4Ch 2Ch

2Ch4Ch

[Cell size]

Set the minimum and maximum size of cell.

Cell size

Min

Max

8

T2 N2

[Dilution factor]

When diluting sample, set the Dilution factor.

CAUTION

!

Factor values for the SCC-Solution is already applied.

Dilution factor

10

1.0

IntroductionGeneral Operation

Introduction

Instructions are provided in this section for preparing the sample with SCC­Solution for use with disposable SOMA Chip for automated somatic cell
count using the ADAM-SCC2.

Please check the procedure of sample preparation and testing below.

For more detailed information, please refer to the next page.

1. Mix the raw milk sample with SCC-Solution.

Milk sample

100 μL

SCC-Solution

100 μL

2. Load the mixed sample. Then, wait 1 minute for the sample settling.

- 2 channel: 23μL

- 4 channel: 13μL

3. Insert SOMA Chip. Get the result.

- Result -

ADAM-SCC2, Automated Somatic Cell Counter 11

IntroductionGeneral Operation

Sample
preparation

Counting Cell

1. Prepare some raw milk sample, SCC-Solution, tube, pipette, and tips.

Raw milk Tube

SCC-Solution

2. Thoroughly mix the raw milk sample.

1. Add 100 μL of raw milk sample and 100 μL of SCC-Solution in the tube.
(1:1 ratio)

Milk sample

100 μL

SCC-Solution

100 μL

2. Mix the raw milk sample and SCC­Solution by turning the tube upside
down 3-5 times.

3. Load the cell sample onto the chip.
Then, wait 1 minute for the sample
settling.

- 2 channel: 23μL

- 4 channel: 13μL

NOTE

!

Ensure that no bubbles enter each channel.

12

13μL

SOMA Chip 4X

IntroductionGeneral Operation

Counting cell

WARNING

!

[Sample loading error]

Be cautious of loading the correct volume of the sample into SOMA Chip.
The instrument will not detect low or high sample volumes.

Correct volume

T1 N1

T2 N2

Low volume

CAUTION

!

T1 N1

T2 N2

Avoid bubbles which may negatively aect the result.

T1 N1

T2 N2

ADAM-SCC2, Automated Somatic Cell Counter 13

IntroductionGeneral Operation

Counting cell

WARNING

!

[SOMA Chip insert error]

Completely insert SOMA Chip face up, in the direction of the arrow on the
slide. The instrument will not detect if slides are inserted incorrectly.
See pictures below for proper insertion.

( O )

Correctly inserted

( X )

Upside down inserted

CAUTION

!

• Please insert or remove the SOMA Chip when the slide holder is fully ejected.

• When the test is nished, please remove the SOMA Chip from the slide holder.

( X )

Not fully inserted

( X )

Wrong end inserted

14

IntroductionMeasure

Run Sample

Enter a title for each channel of the slide.
Click the title section, then scan the cow ID with a barcode scanner to au­tomatically enter as the title.

Start counting process by pressing ‘START’.
It may take about 2 minutes longer for auto focus at the initial test.

9010 7436

While the test is in progress, you can check the cell images of each channel.

ADAM-SCC2, Automated Somatic Cell Counter 15

IntroductionMeasure

Result Analysis

The result will be displayed after being automatically calculated by ADAM­SCC2 software.

CLOSE

No Title Count

T1 120 1.04 x 10E6

N1 125 1.15 x 10E5

T2 130 4.68 x 10E5

N2 143 2.42 x 10E5

16

IntroductionMeasure

Result Analysis

- Error code

E

Error code Cause

E Frames with errors are over 50% of total counting frame.

Frame with error is a frame that contains cells whose

Error frame [#]

diameter is larger than 100μm.

When this error shown in result window, please check
the image.

ADAM-SCC2, Automated Somatic Cell Counter 17

IntroductionData

Data list

①

Control buttons Description

① All Select all data in Data List.

② SEARCH Display the data of the selected date.

③ EDIT

④ IMAGE Check the cell images of each channel.

⑤ SAVE Save the selected data to USB(PDF, Excel, Image).

⑥ DELETE Delete the selected data.

⑦ MAIL

View and edit the data.

Multiple data can be edited with the same settings.

Send the Excel, PDF, and Image les of selected data to
e-mail.

②

③

④

⑤

⑥

⑦

18

IntroductionData

EDIT

①

②

⑤

Control buttons Description

① Sample Edit the sample name.

② Cell size graph View the cell size graph.

③ Cell size setting Set the min/max size of the cell.

④ Dilution Factor

⑤ Frame graph

Set the dilution factor of sample.

Factor values for the SCC-Solution is already applied.

View the counted cell number each frame.

CANCEL

③

④

APPLY

ADAM-SCC2, Automated Somatic Cell Counter 19

Introduction

Data

IMAGE

④

Control buttons Description

① Original Check the original image.

② Counted Check the counted cell image.

③ Frame Select a frame number of the channel.

④ Zoom-in/out

Zoom in and out to check the cell image.

①
②

③

20

Data

Introduction

Data

MAIL

Control buttons Description

① History

② Mail Address

③ Send

Select an e-mail address from the list to send the Excel, PDF,
and Image les of selected data. The e-mail address that
received the les remains in the list.

If you want to receive les by new e-mail, please enter the
e-mail address.

Send the les to a selected e-mail address. Files can be sent
to only one e-mail at a time.

①

②

③

ADAM-SCC2, Automated Somatic Cell Counter 21

IntroductionSetting

Setting

①

②

③

⑥

⑤

④

Control buttons Description

① Count setting

② Dilution factor Set the dilution concentration of the sample.

③ Wi Set the wi to use the e-mail function.

④ Mail Do not change the setting in mail.

⑤ Update Software update through the USB.

⑥ Date&Time Set the current date and time.

⑦ Capacity

Set the conditions in the setting tap before counting.
Refer to page 10 for more information.

Check remaining capacity.

⑦

22

IntroductionSetting

Wi

1. Click the Refresh button.

2. Select the wi.

3. Insert the password of selected wi.

4. Click the Connect button.

CAUTION

!

If connection error occurs, please contact a laboratory facility manager.

Update

1. Prepare the USB with update le.

2. Insert the USB.

3. Click the UPDATE button.

CAUTION

!

• The ‘AdamUpdate’ folder must exist in the root path of the USB folder.

• ADAM-SCC2 can be updated only when the rmware or software le exists in
the ‘AdamUpdate’ folder. The ‘ADAM SCC2.exe’ le should be in the ‘AdamUpdate’ folder.

• Do not rename the ‘AdamUpdate’ folder. The folder name should be ‘AdamUpdate’.

ADAM-SCC2, Automated Somatic Cell Counter 23

Power o

IntroductionSetting

Lock

Power o

Press LOCK before turning o the device.
If there is no operation for 3 minutes, the lock function will be activated

automatically.

When the device is locked, the screen will be changed as shown below.

If you press the power button 2~3 seconds, then ‘Slide to shut down your
PC’ message will appear. Slide down the screen to turn o the power.

24

Introduction

Maintenance and cleaning

Maintenance
and cleaning

1. ADAM-SCC2 does not need regular maintenance.

2. ADAM-SCC2 has no replacement of consumable materials.

3. Please clean the exposed surface of ADAM-SCC2 frequently or before
testing, using a soft cloth and isopropyl alcohol or deionized water.

CAUTION

!

Dispose of wipes in an appropriately labeled solvent contaminated waste
container.

ADAM-SCC2, Automated Somatic Cell Counter 25

IntroductionTrouble shooting

Trouble
shooting

Problem Description Solution

ADAM-SCC2 does

not power up

Inaccurate result

When error

message is shown

(For information on

each error message,

see page 17)

• No power from outlet.

• Bad power cord.

• Cell number may be out of

range.

• SCC-Solution has expired.

• Too high clumped cells.

• When there are too many

frames with errors

(Error message: E)

• Check power source.

• Replace.

• Adjust the number of cells

between 0.05 ~ 1.15 x 10
cells/mL (refer to page 4).

• Check the expired date.

• Try again after vortexing

the cells.

• Check the suspension of

cells if all cells are fully
dissociated into single cells.

• If contaminants except

cells are found, prepare
sample again.

• Error frame • Prepare sample again

except contamination.

6

26

Introduction

Warranty

Warranty

If any defects occur in the ADAM-SCC2 during one(1) year warranty period,
NanoEnTek will repair or replace the defective parts at its discretion without
charge. The following defects, however, are specically excluded:

1. Defects caused by improper operation.

2. Repair or modication done by anyone other than NanoEnTek or
an authorized agent.

3. Damage caused by substituting alternative parts.

4. Use of ttings or spare parts supplied by anyone other than NanoEnTek.

5. Damage caused by accident or misuse.

6. Damage caused by disaster.

7. Corrosion caused by improper solvent or sample.

For your protection, items being returned must be insured against possi­ble damage or loss. NanoEnTek cannot be responsible for damage incurred
during shipment of a repair instrument. It is recommend that you save the
original packing material in which the instrument was shipped. This war­ranty should be limited to the replacement of defective products.

For any inquiry or request for repair service,
Contact [e-mail] or your local distributor.

ADAM-SCC2, Automated Somatic Cell Counter 27

Introduction

Technical Specications

Technical
Specications

ADAM-SCC2

Measuring range

Analysis time

Voltage

Current

Objective lens

LED

Camera

Filter

Weight

Size (W×L×H)

Degree of protection

0.05 ~ 1.15 x 106 cells/mL

< 13 ~ 25 sec/test

DC12V

5A

4 X

4W Green LED

CMOS camera

Excitation lter, Dichroic lter,
Emission lter

7 Kg

227 × 276 × 270 mm

IPX0

: For initial test,
max 2 min/test

Operating environment condition

Temperature

Humidity

Altitude

0 °C ≤ Temperature ≤ 40 °C

10 % ≤ Humidity ≤ 90 %

Altitude ≤ 2,000 m

Transportation & storage environment condition

Temperature

Humidity

Somatic Cell Count Kit

-30 °C ≤ Temperature ≤ 60 °C

10 % ≤ Humidity ≤ 90 %

SOMA Chip

Loading sample vol.

SOMA Chip 2x

T1 N1

SOMA Chip 4x

per test

T2 N2

Measuring sample vol.
per test

*SOMA Chip 2x Kit: please consult your distributor or manufacture for availability.

23 µL/test (SOMA Chip 2X)

13 µL/test (SOMA Chip 4X)

8.6 µL/test (SOMA Chip 2X)

3.4 µL/test (SOMA Chip 4X)

Solutions

SCC-Solution

25 mL

Storage temperature

SOMA Chip

SCC-Solution

0 – 30 °C

2 – 8 °C

Expiration date

SOMA Chip

SCC-Solution

28

2 years

1 year

IntroductionProduct List

Product List

Cat. No. Product Contents Quantity

CRS-K01 SOMA Chip 2X Kit

CRS-K02 SOMA Chip 4X Kit

ADB-500 ADAM Calibration Bead

*SOMA Chip 2x Kit: please consult your distributor or manufacture for availability.

50 pcs SOMA Chip 2X 1

25 mL SCC-Solution 1

100 pcs SOMA Chip 4X 1

25 ml SCC-Solution 2

5 mL Calibration Bead 1

ADAM-SCC2, Automated Somatic Cell Counter 29

IntroductionSafety Precautions

Safety
Precautions

Review and follow the safety instructions below :

• Always ensure that the power supply input voltage matches the voltage
available at your location.

• To avoid the danger of electric shock, install the instrument per the

environmental specications located in “Technical Specications”. If water
or other material enters the instrument, the adaptor, or power inlet,
disconnect the power cord and contact a service person.

• Do not touch the main plug or power cord with wet hands.

• This machine is air-cooled so its surfaces become hot during operation.

During installation and use, leave more than 10 cm (4 inches) free around
the device.

• Do not install the instrument on a slant or a place prone to vibrations or

the risk of instrument malfunction or damage to the instrument will in
crease.

• Never insert any objects (especially metallic) into the air vents of the instru-

ment as this could result in electrical shock, personal injury, and equip­ ment damage.

• Always set the main switch on the power supply unit to OFF before con-

necting the power cord to the wall outlet.

• To avoid a potential shock hazard, always connect the grounding termi-

nal of the instrument and that of the wall outlet properly. The power cord
should be connected to a grounded, 3-conductor power outlet.

• Position the device so that there is sucient length for the cables and

their respective connections.

• Set the main switch to “ O ” (OFF), unplug the power cord, and lock the

stage before moving.

• If the instrument is broken or dropped, disconnect the power cord and

contact an authorized service person. Do not disassemble the instrument.

• Only use authorized accessories.

• Use this equipment only as specied in this manual and as specied in

any documentation associated with its components. Use of the equip­ ment in an unspecied manner may result in damage to the device or
injury to the user.

30

IntroductionSafety Symbols

Safety Symbols

The following symbols are found on the instrument and this document.
Always use the equipment in the safest possible manner.

Symbol

Meaning

Caution & Warning

ON (Power)

This instrument and consumables conforms to the
Declaration of Conformity.

Caution: BIOHAZARD

Protective measures must be used in dealing with
biologically hazardous materials such as carcinogenic
reagents.

USB Connection

LED

LED

Disposal of your old appliance

1.

When this crossed-out wheeled bin symbol is attached to
a product it means the product is covered by the European
Directive 2012/19/EU.

2.

All electrical and electronic products should be disposed of
separately from the municipal waste stream via designated
collection facilities appointed by the government or the local
authorities.

3.

The correct disposal of your old appliance will help prevent
potential negative consequences for the environment and
human health.

For more detailed information about disposal of your old

4.

appliance, please contact your city office, waste disposal
service or visit our web-site, www.nanoentek.com.

This product conforms to UL 61010-1, CAN/CSA C22.2
No.61010-1 “Safety Requirements for Electrical Equipment
for Measurement, Control, and Laboratory Use, Part l: Gen­eral Requirements.” Instruments bearing the TUV symbol
are certied by TUV SUD America Inc to be in conformance
with the applicable safety standard for the US and Canada.

ADAM-SCC2, Automated Somatic Cell Counter 31

IntroductionWarnings

Warnings

After using device, please turn off main power.

1.

If not, it may cause malfunction or may reduce product life.

When turn off the device, be sure lock the device with Lock button.

2.

If not, it may cause mechanical problem or error message when
device is booting.

Item Warning

Battery inside device

Cover

Manual

Sample handling

Waste

• Risk of explosion if battery is replaced incorrectly.

• This battery is not replaceable by user. Refer to an

authorized service person.

• Do not remove cover or dissemble case. There are no

adjustable components inside the instrument.

• If a malfunction is found, refer to an authorized service

person.

• Do not attempt to service the equipment.

• This manual is only available in English.

• Failure to heed warnings may result in injury to service

provider or operator.

• Wear personal protective equipment during sampling

and testing.

• Sample may contain infectious or bio-hazardous agents.

• Use of capped tubes and lint free wipes. Lint free wipes

to be used one time and discarded.

• After using SOMA Chip, appropriately dispose as

bio-hazardous waste.

• Do not reuse SOMA Chip.

32

IntroductionTechnical Support

Technical
Support

Visit the our Website at www.nanoentek.com for :

• Technical resources, including manuals, FAQs, etc.

• Technical support contact information.

• Additional product information and special oers.

For more information or technical assistance, please call or email.

NanoEnTek, Inc.

851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel. [phone]
Fax. [phone]

NanoEnTek America, Inc.

240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA
Tel. [phone]
Fax. [phone]

Email

[e-mail]

Website

www.nanoentek.com

ADAM-SCC2, Automated Somatic Cell Counter 33

ADAM

NESMU-ASC2-001E (V.1.0)

SCC

2

NanoEnTek, Inc.

851-14, Seohae-ro, Paltan-myeon, Hwaseong-si,
Gyeonggi-do, 18531, Korea

Tel :[phone]
Fax:[phone]

NanoEnTek America, Inc.

240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA

Tel: [phone]
Fax: [phone]

Email

[e-mail]

Website

www.nanoentek.com