NanoEnTek JuLi BR User Manual

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Table of contents

Product components

Product overview

Installation

5-6

Description

7-12

Operation

13-51

Focusing

13-19

Adjust the focus

13-14

Auto focus

ROI Auto focus

Confluence

17-18

Capture

Monitoring

20-23

Data

24-32

Open data

24-26

Screenshot

Save to USB

28-29

Rename

Delete

31-32

Counting

33-36

Settings

37-39

Set up parameter 

Update date & time

Control LED power

Update software & firmware

Check the disk space

PC software

40-51

Introduction

Installation

40-41

Function guide

Home

Open data

44-45

Confluence

Edit movie file

Dual view

48-49

Create dual movie

Export data

Cleaning & maintenance

Troubleshooting

53-55

Warranty

Safety precaution

57-58

Product specifications

Technical support

Product components

JuLI™ Br is shipped with the following components. Please check all components listed below when an instrument was delivered. If any items are missing or damaged, contact your local distributor or e-mail sales@nanoentek.com

· Model JULI-BRSC <Scope unit> is only used in conjunction with the <Station unit>.

Scope unit

JULI-BRSC

1 EA

USB drive

4 GB

1 EA

Station unit

JULI-BRST

1 EA

User manual

1 EA

Power cord

4 pcs/set, for U.S./Canada/

Taiwan/Japan, Europe or UK

1 SET

Quick manual

1 EA

Connection cable

3 M

1 EA

Product overview

An increasing number of researchers are using live-cell imaging to study cellular functions. The JuLI™ Br, a smart Bright-cell movie analyzer, was designed for a variety of biological experiments.

JuLI™ Br uses state-of-the-art optics to get live-cell images from various cell culture dishes. It is able to detect the quantified cell confluence results with low variation and make growth curve using image based analysis.

JuLI™ Br is able to capture sequential time-lapse bright images which can be converted to movie files (.avi) automatically. The compact design allows you to install the system in your cell-culture incubator easily. It can be used to compare control and experimental samples using dual systems (*optional), concurrently.

Features of JuLI™ Br, live cell movie analyzer

Compatible with cell-culture incubator

: Operate inside cell-culture incubator with compact & compatible design

Time-lapse image capture & recording movie

: Sequential time-lapse images are stored and can be converted to movie file automatically

Automated quantitative cell confluence analysis

: Quantified cell confluence results with low variation and growth curve

10.1” color LCD touch screen

: Easy-to-use system control

Semi-automated focusing through LCD

: Focus adjustment using focus interface on LCD with knob

Dual system (*Optional)

: Compare the control & experimental data at the same time

Installation

Installing JuLI™ Br

Live-cell images from various cell culture dishes are directly captured in a cell culture incubator. This compact design allows an instrument to be installed in an incubator and prevents contamination by maintaining a sterile environment. Also, the easily-viewed display allows quick and convenient use in a cell culture incubator while capturing time lapse images.

IMPORTANT

JuLI™ Br is optimized into standard size of cell culture incubator.

It is recommend that the temperature should be stabilized in case of small size incubator

before using the instrument.

Avoid exposing JuLI™ Br to UV light.

UV light may degrade components, including plastic. Damage from UV exposure is not

covered under the manufacturer’s warranty.

Always wipe surfaces with ethanol-soaked paper towels, not directly spray ethanol anywhere on JuLI™ Br.

Dimensions of Scope unit

Width

Depth

Height

Weight

Size

300 mm

190 mm

188 mm

4 kg

Cell culture incubator

1. Place the instrument on a flat, dry and level surface after unpacking the instrument. Allow at least 10 cm (4 inches) of free space at the back of JuLI™ Br for proper ventilation and prevention of overheating of electronic components.

2. Plug the supplied power cord into JuLI™ Br.

3. Connect scope unit to station unit using supplied

connection cable.

(* For dual monitoring, 2nd scope unit also should be connected to station unit. )

4. Plug the power cord into the electrical outlet. Be sure to only use the power cord supplied with the instrument. Powering the instrument with an unapproved power cord may damage the instrument.

5. When JuLI™ Br is ready to use, start JuLI™ Br by pressing the Power button on the station unit.

Installation

Installing JuLI™ Br

2. 3. 3.

IMPORTANT

Connect to the 2

scope unit after connecting to the 1

scope unit completely

Description

Front view

LCD touch screen

: located at the front of the instrument, it contains buttons for necessary functions and displays bright field images.

Focus knob

: is used to adjust the image quality to obtain better bright cell images.

Stage

: place sample here.

White LED

: provides bright image viewer.

White LED

Stage

< Scope unit >

< Station unit >

Neck

Focus knob

LCD

touch screen

Description

Side view

Power button

: turn an instrument on and off by pressing the Power button.

USB Port

: insert a USB drive to save and transport images to PC.

Display port

: connect to external monitor.(resolution: 1366 X 768 pixels)

: make sure to turn off an instrument before connecting.

Connection port

: port to connect scope unit with station unit .

< Scope unit >

< Station unit >

Connection port

USB port

Display port

Power button

Description

Rear view

Power inlet

: connects JuLI™ Br to an electrical outlet using the supplied

power cord and the appropriate plug (based on the electrical outlet configuration in your country).

On/off switch

: the main power switch. It is not necessary to use the on/off switch for

day-to-day operation of the instrument.

Connection port

: port to connect scope unit with station unit.

Fan

: machinery cooling system.

< Scope unit >

< Station unit >

Fan

Powe r inlet

On/off switch

Connection port

The user interface of JuLI™ Br, Live cell movie analyzer provides new tools to expand cellular research through proprietary software. Insightful data can be acquired, such as proliferation assays.

Description

User interface

Focusing menu

User interface

Monitoring menu

Zoom in

Navigation

Zoom out

Auto focus

Focus

interface

Exposure

Brightness

D-Phase

Capture

Confluence

Focusing menu

Graph

Scope unit

channel

Monitoring setting

Monitoring data infomation

Monitoring movie

REC. button Stop button

Monitoring menu

Scope unit channel

Sacle bar

Description

User interface

Data menu

User interface

Settings menu

Monitoring movie

Monitoring data information Zoom bar

Multi select button

Delete button

Save to USB button

Rename button

Data menu Graph Data list

Sensitivity & Background level

Disk space Software

version

Firmware version

OS version

LED power

Data & Time information

Settings menu

Scope unit channel

Description

User interface

Counting menu

Counting menu is a JuLI™ Br Counting software which can be used with various cell types giving the accurate and fast counting results in the bright mode.

1. Bright counting : to check for the cell viability by trypan blue staining method.

Counting menu

Features of JuLI™ Br Counting S/W:

Applicable for broad range of cell sizes and types:

Provides data on cell size and is compatible with a wide variety of eukaryotic cells without the need for any special changes between large or small sizes.

Measures cell measurement ranging from 1 × 10

to 1 × 107 cells/mL, optimal

measurement range from 1 × 10

to 4 × 106 cells/mL, and cells with sizes ranging

from 5 µm to 60 µm.

Innovative Auto-focus technology:

Allows to get the optimized focus without variation of manual focus.

Accurate and fast counting result:

Provides counting result without focus variation and regardless of clumped cells in less than 10 seconds

(Guaranteed the results and counting time using Auto-focus function.).

Brightness bar

Exposure bar

Scope unit channel

Graph

Counting result

Zoom in/out

Save to USB button

Focus interface

Auto focusing button

Setting bar of min. & max. cell size

Counting/Preview button

Operation

Focusing

Adjust the focus

1. Press the Power button to start JuLI™ Br. The main screen will be displayed.

2. Place the sample on the stage.

3. Adjust the focus for each scope unit if 2

unit is connected to

station unit. Press Channel tab to select each scope unit.

4. To adjust Illumination intensity, use the Exposure and Brightness bar on the touch screen.

Touch the arrow button (

◀|▶

) for more

minute adjustment.

5. If desired, use Zoom in button to magnify the desired region.

(» The scale of enlargement can be checked by

pressing Scale bar tab.)

6. If focus of viewing region should be moved, tap a point on navigation window or drag preview window.

While viewing cells, use the Focus knob or Focus interface

to further adjust the focus.

Operation

Focusing

Adjust the focus

· Type of focus interface would be switched by tapping circle point.

· Coarse focus is larger knob which moves up or down rapidly to

get the sample into coarse focus.

Fine focus is smaller knob which moves short distances slowly

and is used to get the sample into sharp focus.

The images below show the reference images to adjust focus.

< coarse focus interface >

Good setting

Exposure 4 Brightness 1

Normal setting

Exposure 6 Brightness 3

Bad setting

Exposure 6 Brightness 5

< fine focus interface >

Du-145 / Parameter A

Operation

Focusing

Auto focus

1. Adjust the focus of JuLI™ Br. (Refer to page 12-13)

2. After adjusting focus, press the Auto focus button to get more

accurate focus without individual variations.

· The Auto focus is the auto-focusing function which automatically senses the

focus optimized for the analysis software of the JuLI

™ Br

IMPORTANT!

It is strongly recommended to use Auto focus function after adjusting a focus. If not, it might be the cause of incorrect counting results or viability

Operation

Focusing

ROI Auto focus

1. Adjust the focus of JuLI™ Br. (Refer to page 12-13)

2. Touch the region of interest cell by hand on the screen

· The ROI Auto focus is the auto-focusing function which automatically

senses the focus optimized for the analysis software of the JuLI

™ Br

IMPORTANT!

It is strongly recommended to use ROI Auto focus function after adjusting a focus. If not, it might be the cause of incorrect counting results or viability

Operation

Focusing

Confluence

1. Adjust the focus of JuLI™ Br using Focusing menu. (on page 11-12)

2. Press Confluence button.

3. Value of confluence would be shown.

· If the value of confluence should be more accurate, try again after

adjusting parameter in Setting menu. (on page 29)

Parameter is the recommended value for corresponding to cell line.

· If confluence should be recalculated, press Confluence button after

pressing Preview button first.

4. Press the Capture button to acquire image with confluence.

5. Type the name of image that should be saved.

Then, press Save button.

Operation

Focusing

Confluence

6. Check confirmation message ‘Capture complete’. Saved data can be opened in Data menu. (on page 20)

The images below show the guideline for focus to obtain accurate cell confluence.

Du-145 / Parameter A

Good setting

Exposure 4 Brightness 1

Normal setting

Exposure 6 Brightness 3

Bad setting

Exposure 6 Brightness 5

Confluence 35.11%

Confluence 36.22%

Confluence 43.03%

1. Adjust the focus of JuLI™ Br using Focusing menu. (on page 11-12)

2. Press the Capture button to acquire the image.

3. Type the name of image that should be saved. Then press Save button.

4. Check confirmation message ‘Capture complete’. Press OK button.

5. Saved data can be opened in Data menu. (on page 20)

Operation

Focusing

Capture

1. Each scope channel can be viewed by pressing Channel tab if 2

scope unit is connected to station unit.

2. Adjust the focus of JuLI™ Br and select the region that should be monitored using Focusing menu. (on page 11-12)

Press Setting button.

3. Press the Name form to type file name for saving images or movies. Then press Save button.

Operation

Monitoring

4. Choose the monitoring mode among Growth rate, Wound healing and Movie only.

· Movie only means just monitoring cells without a confluence.

5. Press the Total time to set up the total running time. Press the Interval to set the time interval. Select ‘on’ for AVI file to create AVI file with time lapse images.

Select scope channel should be monitored.

· Choose both channel 1 and channel 2 in case of dual monitoring

IMPORTANT!

Warm up scope unit for 1 hour in incubator before making a movie. If not, it might be the cause of uncertain data or unclear movies.

IMPORTANT!

Cell culture flask should be wetted by the culture media before making movie.

Operation

Monitoring

· Time interval should be set up more than 1 minute for the best quality

result in case of growth rate and wound healing.

but in case of dual monitoring, more than 5 minutes is recommended.

· The number of total images will be displayed automatically when data

value of “Total Time” and “Interval” are set up.

Press the Apply button to save all options.

· If the disk volume of the instrument is not enough to save recording

data, then the error message as below will be shown.

Please try again after deleting data in Data menu. (on page 20)

7. Press the Rec. button to begin recording. JuLI™ Br displays live cell images continuously during recording.

Operation

Monitoring

· Dual scope channel can be checked at once by pressing

Dual view

button

when 2nd scope unit is connected to station unit.

To close dual view mode, double click it.

· If the movie should be finished while recording,

press

Stop button

Then the confirmation message as below will be shown.

Press

OK button

· Unexpected problems such that the power is off suddenly or

the scope unit is not connected to station unit while movie recording

could be happened.

Then the error message as below would be shown after restarting

the instrument.

Recorded data before getting in problem is saved in the disk of

instrument temporarily. So press

OK button

if the movie recording

should be continued.

8. Recorded data can be opened in Data menu. (page 20)

Operation

Monitoring

Operation

Data

Open data

1 All of saved data could be checked in Data menu.

· Monitoring data including movie file and time lapse images were saved

in each of folder set up by user. And other data except monitoring are

saved in a default folder, ‘Captured Images’.

2. There are sub folders in case of dual monitoring data. Each control sample and experimental sample data is in the sub folder.

Default folder for captured images

Main folder

Monitoring data folders set up by user

Sub folder

If you double tap main folder of dual monitoring data, summary

data view will show up.

3. Data can be opened or shown by double tapping folder name in List category or tapping folder name once and pressing

Result category.

4. Recorded movie or image file can be shown by tapping its file name. Movie file can be checked by pressing Play button.

Operation

Data

Open data

5. Magnify movie screen or image screen widely for convenience.

Operation

Data

Open data

1. Insert USB drive into USB port.

· If a USB drive is not inserted into USB port, screenshot function

can not be used.

2. Press Screenshot button.

3. Select the desired capture region using moving icon.

4. Select the screen size of snapshot using size icon.

· Snapshot capture process can be canceled

by pressing cancel icon

5. After then double tap the selected region.

6. Type the name for saving it, then press Save button.

7. Check confirmation message ‘Screenshot is saved successfully!’ .

Operation

Data

Screenshot

1. Insert USB drive to USB port.

· If a USB drive is not inserted into USB port, you can not use this

function.

2. Select the folder or file by tapping it once.

3. Several folders or files can be selected at once using Multi select button. If the wrong file is selected, press Multi select button again.

4. Press the Save to USB button.

· Only main folder can be saved, not sub folders

individually in case of dual

monitoring data.

Operation

Data

Save to USB

5. Confirmation message ‘Save complete’ is shown, saving process is completed.

· Experimental sample data (channel 2) is included in control sample

data (channel 1) folder in case of dual monitoring

Operation

Data

Save to USB

Operation

Data

Rename

1. If you want to change folder or file name, tap the file or folder.

· Rename function can be used for folders of monitored data and files

in default folder ‘Captured Images’. Not for each files in monitored data

folder.

2. Then, press Rename button.

· Only main folder can be renamed, not sub folders individually in case of

dual monitoring data.

3. Enter new name of folder or file using the keypad display. Then press Save button.

4. Check the confirmation message ‘Rename complete’ and renamed folder or file in List and Result category.

1. Select folder or file which should be deleted from the instrument.

· Delete function can be used for folders of monitored data and files in

default folder ‘Captured Images’ , but not for each files in monitored

data folder.

· To delete several folders or files at once, select all of them after pressing

Multi select button.

2. Delete the folder or file with Delete button.

· If ‘Captured Images’ folder is selected,

all of image files in the folder will be deleted, but not the folder.

· Only main folder can be deleted, not sub folders individually in case of

dual monitoring data.

3. Press OK button when confirmation message shows up.

Operation

Data

Delete

4. Check confirmation message ‘Delete complete’.

Operation

Data

Delete

· Mix well 10 µL of sample and the 10 µL of 0.4% trypan blue stain (Bright mode)

1. Load 10 µL of sample mixture on the counting slide (side A) using a pipette. The two chambers of the slide and labeled as "A" and "B" for easy tracking of your samples. Each chambers is counted seperately.

2. Put the counting slide with the slide holder on the scope unit.

Operation

Counting

Sample-

preparation

1. Adjust the focus JuLI™ Br (Refer to page 12-13)

2. After adjusting focus, press the Auto focus button to get more accurate focus without individual variations.

· The Auto focus is the auto-focusing function wich automatically senses the focus optimized for the analysis software of the JuLI™ Br and provides the precise and rapid counting results.

· The example images of HeLa cells show the reference calue for adjusting focus.

Operation

Counting

Auto focus

Focus by user in various cases

Dark focus Normal focus

Bright focus

recommend for

JuLI

Br counting

Apply

IMPORTANT!

It is strongly recommended to use Auto focus function after adjusting a focus. If not, it might be the cause of,

- taking more time for counting (more than 10 seconds)

- incorrect counting results or viability

1. Adjust the focus of JuLI™ Br (Refer to page 12-13). If necessary, press the Auto focus button to get more accurate focus without individual variations.

2. Press the Counting button for the cell counting.

· The Br counting button is activated in the Bright mode.

· If necessary, set the dilution factor corresponding to the experiment condition

(Refer to page 42).

3. The counting results including the total cell, live cell, dead cell, and average cell size show up.

Operation

Counting

Bright counting

(Stained by trypan blue)

Gating background

Graph

Dead cell (red bar)

Live cell (blue bar)

Counting results

1. Connect USB drive to the station unit.

· If a USB drive is not connected to the station unit, you can not use this function.

2. Press the Save to USB button.

3. Type the folder name in the blank. Then press the Save button.

· When a user presses the Save button, the user will acquire the images and the

counting result file for one folder automatically. In a folder, there are one original cell

image, one cell counting image marked with different color for the viable and dead

cells, one screen shot image with cell counting result data and also, one csv file of

counting result data.

Bright

4. If the confirmation message is shown, the saving process is completed.

Operation

Counting

Save to USB

< Bright counting result save > < Fluorescence counting result save >

Operation

Settings

Set up parameter

1. Setting menu works for each scope unit if 2

unit is connected

to station unit. Press Channel tab to select each scope unit.

Set the recommended parameter value corresponding to cell line. Default value is A.

Sensitivity :

refers to the contrast of the objects from the back ground.

Increased sensitivity provides better recognition of fainter objects

in bright field.

Background level:

decreased background level provides better recognition of fainter objects

in bright field.

Cell line

HepG2 GH3 Hep3B A549 MCF7 SH-SY5Y SCN2.2 NIH-3T3 F9 HeLa DU-145 LNCaP

U-2 OS ADSC

Recommendation

Sensitivity level 9 Background level 2

Sensitivity level 10 Background level 2 Sensitivity level 10 Background level 8

Parameter

A(Default)

Operation

Set up local date & time with up and down button.

Adjust the LED power intensity, tapping the bar to point. Default LED power is 10.

1. Periodically, NanoEnTek adds functionality and other improvements to JuLI™ Br user interface. We recommend keeping your JuLI™ Br update with the latest software and firmware using update button.

If you have any questions about software updates, contact sales@nanoentek.com.

2. Visit JuLI™ Br website at www.nanoentek.com to download updated software which is supplied as a .zip file in your desktop.

3. Unzip the .zip file and save the software in a USB driver.

4. Insert the USB drive into the USB port on JuLI™ Br.

Setting

Update software & firmware

Setting

Update data & time

Setting

Control LED power

Operation

Setting

Update software & firmware

5. Check if the version of software and firmware are the latest one. Press Update button. The update process takes a few seconds.

6. After updating, Press OK button to restart JuLI™ Br automatically.

Check the disk space of JuLI™ Br. If no disk space left, saved data should be deleted in Data menu.

(on page 20)

Setting

Check the disk space

Operation

PC software

Introduction

PC software

Installation

PC software is designed to manage data from JuLI™ Br.

· User can review saved image from JuLI™ Br in the PC.

· User can recalculate confluence of all images and edited movies.

To install PC software, follow the directions as below : Check to ensure that the PC is connected to Internet and then the PC software could be installed.

1. Connect the supplied USB driver to the computer. Then open the file “JuLI™ Br PC software”

2. The start-up dialogue of the software, as shown like left image, will appear.

3. Click ‘Next’ to start installation.

Operation

PC software

Installation

5. After choosing installation folder, click “Install” to proceed with the installation.

4. If you want to change installation folder, click ‘Browse’ and choose the location that you want.

6. The computer activates the “Installation of the software”

7. If the installation is successful, the PC software program icon will appear on your desktop.

Operation

PC software

Function guide

When PC software program starts, the interface as below image will be shown.

Home

Zoom bar Image information Preview window Graph Data list

Confluence Movie

Open

Save as

ExportCreate

dual movie

Preview window

Dual view

Operation

PC software

Home

1. Press Insert scale bar button to insert scale bar on viewing image, if it is necessary.

Click Save with scale bar button to save image with scale bar information.

Operation

PC software

Open data

1. Click the Open button.

· Folders and files can be opened by dragging and dropping on

List region directly.

2. Browse the folder or files and select it.

3. If data opened successfully, data list could be checked out as

below image.

Operation

PC software

Open data

4. Click the name of image in data list. Preview window will show the image and movie.

· In case of dual monitoring data, control sample folder

and experimental sample folder can be checked easily

using Channel tab.

5. The image can be magnified by tapping zoom bar.

6. Select the viewing region by dragging preview window.

Operation

PC software

Confluence

1. Click the confluence menu button.

2. Select one image that needs recalculated confluence value

from data list.

3. Adjust the image by changing parameters; recommended sensitivity level and background level corresponding to cell line.

4. Click the Confluence button. the value of confluence would be recalculated by new sensitivity level, and background level.

5. If the confluence value from step 4 is good enough to use, then check Apply box for recalculating confluence value of all images in same folder by new sensitivity and background level.

6. Confluence graph would be redrawn.

7. Click Save as button

if recalculated data should be saved.

Parameter

A(Default)

Cell line

HepG2, GH3, Hep3B, A549, MCF7, SH-SY5Y, SCN2.2, NIH-3T3, F9, HeLa, Du-145 LNCaP

U-2 OS, ADSC

Recommendation

Sensitivity level 9 Background level 2

Sensitivity level 10 Background level 2 Sensitivity level 10

Time ( Hour : Min ) Time ( Hour : Min )

Operation

PC software

Edit movie file

1. Recorded movie can be shortened easily through PC software. Set the start point and end point by moving the bar.

2. Check Apply box to edit the movie.

3. Click Save as button if edited movie should be saved.

4. Select the folder to save edited movie and press OK button.

Time ( Hour : Min )

1. Dual monitoring data can be checked at once using Dual view menu button. Click Dual view menu button.

2. Summary of dual monitoring data will be shown.

3. Individual movie files from control sample and experimental sample can be made into one movie using Export dual movie button.

Operation

PC software

Dual view

4. Type file name for merged movie and click Save button.

5. Merged movie file will be created after merging process is

done.

Operation

PC software

Dual view

1. Individual movie files from 2 different single monitoring data can be made into one movie using Create dual movie button.

2. Select 2 different single monitoring data folder for making movie.

· Selected movie files should have been monitored under the same

interval and total time.

3. Click ‘Make New Folder’ button and type folder name for dual movie.

3. Dual movie file will be created after working process is done.

Operation

PC software

Create dual movie

1. Click Export button. Information of each image in folder can be exported.

2. Select the folder where exported file should be saved. Type the file name to save exported file.

3. Exported file can be found in folder.

Operation

PC software

Export data

Cleaning & maintenance

Clean the surface of JuLI™ Br instrument with a damp cloth. To clean the LCD screen, turn off JuLI™ Br instrument, disconnect the power cable, and clean the LCD screen with a soft cloth lightly moistened with LCD cleansing detergent. Cleaning the screen with excessive force can damage the LCD the screen. Wipe the screen dry immediately.

If liquid spills on JuLI™ Br, turn off the power immediately and wipe dry.

JuLI™ Br does not need regular maintenance. To troubleshoot problems with JuLI™ Br, contact Technical Support (page 59).

IMPORTANT

Never disassemble or service JuLI™ Br by yourself.

Unauthorized repairs may damage JuLI™ Br or alter its functionality, which will void your warranty. Contact sales@nanoentek.com or your local JuLI™ Br distributor to arrange for service.

Always wipe surfaces with ethanol-soaked paper towels.

Do not directly spray ethanol anywhere on JuLI

Br.

Avoid exposing JuLI™ Br to UV light

UV light may degrade components, including plastic. Damage from UV exposure is not covered under the manufacturer’s warranty.

Troubleshooting

· Check on/off switch on back side of unit.

· Check power source or contact your distributor.

· Touch the LCD screen with your finger .

· Check if power supply is connected and power switch is on.

· Reset the power button.

· Check the connection between scope and station.

· Connect connection cable to the 2

scope unit after

connecting to the 1

scope unit completely.

· Check whether the focus interface is inactive or not.

· Move the focus knob to the opposite side.

· Re-optimize the brightness and exposure value.

· Reset the power button.

· Swipe the stage with cotton swab carefully.

· Eliminate any dust on culture dish.

· Check for and remove any condensation on the lid of the culture dish.

· Warm up the instrument during 1 hour before monitoring. (power should be on when warming up the instrument)

· Cell culture flask should be wetted by the

culture media before making movie.

· Eliminate dust on a culture dish.

· Check for and remove any condensation on the lid of the culture dish.

Installation JuLI

Br does not power up

LCD screen is black

Focusing and monitoring menu are not activated Get an error message during the connecting the 2

scope unit

Focus Focus knob or focus interface doesn’t work

Monitoring Poor image (Too dark and too bright) Dirty image

Time-lapse images become dark and bright

Troubleshooting

· To prevent any problem such as shaking of culture dish or inflowing light, pay special attention when you open or close incubator door during monitoring.

· Please check the minimum interval (on page 18).

· Check the unused volume of the hard disk.

· Recording files are going to be saved as the last state before recording stopped.

· Create movie file with time lapse images using JuLI

Br PC software program.

· Download 'JuLI Br Update' folder on NanoEnTek website. (www.nanoentek.com)

· Check the files as below image. Then download 'JuLI Br Update' folder on NanoEnTek website. (www.nanoentek.com)

Monitoring

Time-lapse images become dark and bright

Can’t set the interval

Problem for saving movie file

Can not continue recording. Do you want to save the recording files?

Data Can not play the movie properly

Setting 'JuLI Br Update' folder does not exist in the USB drive.

The update files do not exist in the USB drive.

Troubleshooting

· Load the full folder to PC software program, not just avi file.

· Check the interval and total time and match them with same conditions.

· Confluence is not measured on Movie only mode. However, movie can be edited using PC software program.

· Connect scope unit with station unit using connection cable again.

· Install the codec provided by JuLI Br USB drive or

NanoEnTek website.

· Check the resolution of the outer monitor. (resolution 1366 X 768 pixels)

· Make sure to turn off instrument before connecting.

PC software Movie doesn’t play

Can’t create the dual movie using 2 different single monitoring data

Graph is not be shown on the movie tap

ETC Fail to capture

Movie doesn’t play on windows media player

Can’t transmit the screen to external monitor through display port

Warranty

NanoEnTek provides 1-year warranty service for defects of material and workmanship.

If any defect occurs in JuLI

, NanoEnTek provides free repair service for the defective

parts at its discretion.

The following defects, however, are specifically excluded:

1. Defects caused by improper operation.

2. Repair or modification done by anyone other than NanoEnTek or an authorized agent.

3. Damage caused by substituting with alternative parts.

4. Use of fittings or spare parts supplied by anyone other than NanoEnTek.

5. Damage caused by accident or misuse.

6. Damage caused by disaster.

7. Corrosion caused by improper solvent or sample.

For your protection, JuLITM Br units being returned must be insured against possible damage or loss. NanoEnTek cannot be responsible for damage incurred during shipment of a defective instrument. It is recommend that you save the original packing material in which the instrument was shipped. This warranty is limited to the replacement of defective products.

For any inquiry or request for repair service, contact sales@nanoentek.com or your local distributor.

Safety precautions

Review and follow the safety instructions below :

· If water or other material enters the instrument, adaptor, or power inlet,

disconnect the power cord and contact a service person. For operating environment,

refer to Product Specifications (page 58).

· Do not touch the main plug or power cord with wet hands.

· Always ensure that the power supply input voltage matches the voltage

available at your location.

· This instrument is air-cooled and its surfaces may become hot during

operation. When installing leave a space of more than 10 cm (4 inches)

around the instrument and do not place any objects between the instrument

and the walls.

· Do not install an instrument on a slant or a place prone to vibrations, which

induces the risk of malfunction or damage of the instrument.

· Never insert any objects into the air vents of the instrument as this could

result in electrical shock, personal injury, and equipment damage.

· Plug the power cord firmly into the wall outlet and AC adapter.

· To avoid potential shock hazard, make sure that the power cord is properly

grounded.

· Be sure to position the equipment such that it is easy to disconnect.

· Turn off the instrument before unplugging the power cord and/or moving the

instrument.

· If the instrument is dropped or broken, disconnect the power cord and

contact a service person. The warranty will be void in case of disassembly.

· Use only authorized accessories (adaptor, power cord, and USB drive).

Warning

Class A equipment is intended for use in an industrial environment.

In the documentation for the user, a statement shall be included drawing

attention to the fact that there may be potential difficulties in ensuring

electromagnetic compatibility in other environments, due to conducted as well as

radiated disturbances.

Review and follow the safety instructions below :

Safety precautions

Meaning

Caution & Warning

Protective earth (Ground)

This instrument and consumables conforms to the Declaration of

Conformity.

This equipment has been tested and found to comply with the limits for a

Class A digital device, pursuant to Part 15 of the FCC Rules.

These limits are designed to provide reasonable protection against

harmful interference when the equipment is operated in a commercial

environment.

This equipment generates, uses, and can radiate radio frequency energy

and, if not installed and used in accordance with the instruction manual,

may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause

harmful interference in which the user will be required to correct the

interference at his own expense.

Symbol

FCC Compliance

Environmental conditions

Instrument specifications

Product specifications

AC 100 - 240Va.c., 1.9A, 100W

50 / 60 Hz

Indoor use only

5 - 40 °C

20 - 95 %

Device

JULI-BR04 (Single set, 1 Scope & 1 Station)

JULI-BRD04 (Dual set, 2 Scopes & 1 Station)

JULI-BRSC (2nd Scope)

Accessory

JULI-BRCM (Counting starter kit)

JULI-BRTB (XY Stage)

Objective 4X and digital zoom (~400X)

2560 x 1920 pixels (5M)

JPEG (image), AVI(movie), CSV(raw data)

10.1" LCD touch screen

White LED

Scope unit : 300 x 190 x 188 mm, 4kg

Station unit : 282 x 285 x 160 mm, 3.2kg

320 GB Hard drive

4 GB USB drive

Electrical input

Frequency

Installation site

Operating

temperature

Maximum relative

humidity

Cat. no.

Magnification

Image resolution

Exported formats

Display

Light source

Dimension & weight

Storage

Technical support

Visit the our Website at www.nanoentek.com for :

Technical resources, including manuals, FAQs, etc.

Technical support contact information

Additional product information and special offers.

For more information or technical assistance, please call or email.

NanoEnTek Inc. (HQ)

12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 08389, Korea Tel : +82-2-6220-7940 Fax : +82-2-6220-7721

NanoEnTek USA Inc.

5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA Tel : +1-925-225-0108, +1-888-988-0108(Toll free) Fax : +1-925-225-0109

sales@nanoentek.com

Website

www.nanoentek.com

All the materials in this user manual are protected by Korean and international copyright laws. They cannot be reproduced, translated, published or distributed without the permission of the copyright owner.

JuLI™ Br User Manual

Website : www.nanoentek.com

E-mail : sales@nanoentek.com

Manufactured by : NanoEnTek Inc.

12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 08389, Korea

Tel : +82-2-6220-7940

Fax : +82-2-6220-7721

The information in this manual is described as accurately as possible. The changes involved in firmware and software may be applied without notification.

Documentation : NESMU-JUB-001E

Revision History : V.0.0 Date : Oct 2012

V.1.0 Date : Jan 2013

V.1.5 Date : May 2013

V.1.6 Date : Jan 2014

V.2.0 Date : Nov 2015

NanoEnTek Inc. (HQ)

12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 08389, Korea

Tel. +82-2-6220-7940 Fax. +82-2-6220-7721

NanoEnTek USA Inc.

5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA Tel : +1-925-225-0108, +1-888-988-0108(Toll free) Fax : +1-925-225-0109

sales@nanoentek.com

Website

www.nanoentek.com

NESMU-JUB-001E (V.2.0)

NanoEnTek JuLi BR User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual