Nanoentek EVE Plus User Manual

Automated cell counter

User Manual

Copyright NanoEnTek, Inc. All rights reserved.

All the materials in this user manual are protected by Korean and international copyright laws. They cannot be
reproduced, translated, published or distributed without the permission of the copyright owner.

EVE™ PLUS User Manual

Website: www.nanoentek.com
E-mail: [e-mail]

Manufactured by

NanoEnTek, Inc.

851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel. [phone]
Fax. [phone]

NanoEnTek America, Inc.

240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA
Tel. [phone]
Fax. [phone]

The information contained in this document is subject to change without notice.
Firmware and software changes and updates may change without prior consent or notication.

Copyright © 2019 by NanoEnTek Inc.

All rights reserved. Published in Korea.

Documentation: NESMU-EVE2-001E

Revision history: V.0.0 JUN 2019

Table of contents

Introduction

Product contents

Product overview

Features and benets

Product description

Front view

Side view

Rear view

Installation

Install EVETM PLUS

Activation code

Setting

Setting menu

Mode

Parameter

Calibration

Date

Operation

Recommend actions

Cell counting (Auto focus)

Cell counting (Manual focus)

Maintenance and cleaning

2

3

4

Troubleshooting

Warranty

Product specications

Ordering information

5

5

6

7

8

9

9

9

12

14

15

16

19

Safety precautions

Safety symbols

Technical support

30

31

33

34

35

36

38

39

DATA

Check the image

Edit the histogram

Data export (Report & image)

Data export (CSV le)

Data export (Mail)

24

26

27

28

29

Introduction

Product
contents

EVE™ PLUS is shipped with the following components.
Please check that all items listed below were shipped, receiving the instru­ment. If any items are missing or damaged, contact your local distributor or
e-mail [e-mail].

Automated cell counter

1 EA

Cat. No. EVE-MC2 Cat. No. EVS-050

Power cord

Europe/Korea, USA/Japan, Australia,

UK, or China

1 SET

Cell counting slides

with 1.5 mL of Trypan blue (0.4%)

1 BOX (50 slides/box)

Adaptor

1 SET

User manual

1 EA

User

manual

2

Quick manual

1 EA

Quick

manual

Introduction

Product
overview

EVE™ PLUS Automated cell counter uses state-of-the-art optics and image
analysis for automatic cell counting. EVE™ PLUS is a benchtop counter de­signed to measure cell count and viability (live, dead, and total cells) accu­rately and precisely, using the standard trypan blue technique.

Using the same amount of sample that you currently use with the hemo­cytometer, EVE™ PLUS takes less than 1 second per sample for a typical cell
count with manual focus option and is compatible with a wide variety of
eukaryotic cells and provides information on cell size.

EVE™ PLUS is supplied with disposable EVE™ Cell counting slides that con­tain two enclosed slides to hold the sample to allow you to measure two dif­ferent samples or perform replicates of the same sample. The cell count­ing occurs in the central location on the counting slide and the volume
counted is 0.4 µL, the same as counting four (1 mm × 1 mm) squares in a
standard hemocytometer.

EVE™ PLUS, Automated Cell Counter 3

Introduction

Features and
benets

• User-friendly, compact design for simple, fast, automated cell count and

viability measurements within 1 second.

• It provides data on cell size and is compatible with various types of eu-

karyotic cells, regardless of whether the cell size is small or large.

• Measures cell concentrations ranging from 1 × 10

cells with sizes ranging from 5 µm to 60 µm.

• Provides the clumpy cell counting function to get more accurate results.

• Uses disposable cell counting slides that you can eliminate washing steps

and cross contamination between samples with.

• Up to 500 test results are automatically saved in the DATA tab.

• Presents comprehensive data with graphical reports and as a .CSV

(comma separated value) le for sample comparisons.

4

to 2 × 107 cells/mL and

4

Product description

Front view

Side view

Item Description

① LCD touch screen Located in the front of the instrument contains buttons

② Slide port The slide port is used to insert the EVE™ Cell counting

Item Description

③ Image adjustment

(focus) knob

④ USB port

⑤ Power button

for all the functions needed and displays data from the
cell count.

slide containing the sample with trypan blue stain into
the counter for analysis.

The image adjustment (focus) knob is used to adjust
the image quality to obtain better contrast between
live (bright centers) and dead (dark blue centers) cells
by manually. This is important to obtain accurate cell
counts and viability measurements.

The USB port allows you to transfer and save the cell
count data and image to your computer for record
keeping and printing purposes. Any other standard
USB drive can be used for data transfer.

To turn the power on and o, press and hold for 3
seconds. The unlighted status indicates that the
instrument is o; the red status light indicates that the
instrument is on.

② Slide port

① LCD touch screen

⑤ Power button

④ USB port

③ Image adjustment knob

EVE™ PLUS, Automated Cell Counter 5

Product description

Rear view

The front view showing various parts of the ADAM-MC2 is shown below:

Control buttons Description

① USB port The USB port allows you to transfer and save the cell count

data and image to your computer for record keeping and

printing purposes. Any standard USB drive can be inserted into

the USB port for data transfer.

② Power inlet

Connect the counter to an electrical outlet using the supplied

power cord and the appropriate plug, based on the electrical

outlet conguration in your country.

① USB Port

② Power inlet

6

Installation

Install
EVE™ PLUS

1. After unpacking the instrument, place the instrument on a at and dry sur­face.

2. Connect adaptor and power cord,
then plug the power cord to EVETM PLUS.

3. Plug the power cord into the electrical outlet. Be sure to use only the
power cord supplied with your instrument. Powering the instrument with an
unapproved power cord may damage the instrument.

4. When you are ready to use, start the EVE™ PLUS by pressing and hold 3
seconds the Power button.

5. When the instrument is turned on, the startup screen is displayed. Here
you can proceed immediately to cell counting, set up the instrument for cell
types, or adjust the parameter for cell counting.

EVE™ PLUS, Automated Cell Counter 7

Installation

Activation
code

To use the EVE™ PLUS for the rst time, you need the activation code, which
is in the EVE™ slide box enclosed with the instrument. Entering the pro­vided code into the pop up window allows normal cell counting.

This code has a limit on the number of uses, and it is possible to use the
counting function of 200 times per code.

If the limit is exceeded, this popup window will reappear.
In this case, you must enter a new code from the new box.

* This code is intended to provide accurate calculation results using correct consumables.

8

Setting

Setting menu

Mode

Parameter

Press SETTING from the startup screen to display settings.
The setting menu allows you to set up the following:

• MODE to operate the instrument for cell counting (choose single cell) or
clumy cell counting (choose CLUMP CELL)

• PARAMETER (see below and next page for details)

• CALIBRATION to calibrate the instrument image background level (page 12)

• UPDATE to install new rmware versions as they become available

• DATE to set up date and time (page 14)

• WIFI to connect to the internet to send results via mail

Select count mode for cell count­ing. Please choose counting mode
(SINGLE CELL or CLUMP CELL).

1. Press PARAMETER from the setting screen to display
parameters screen.

Parameter function allows you to change the image analysis algorithm for
specic or mixed cell types, and the specic parameters must be deter­mined empirically.

EVE™ PLUS, Automated Cell Counter 9

Setting

Parameter

2. The parameters are described below:

[Sensitivity]

Sensitivity (refers to the contrast of the objects against the background).
Adjusting the sensitivity higher makes instrument more sensitive to ob­jects; useful for cells that do not stain well with trypan blue while adjust­ing the sensitivity lower makes the instrument less sensitive and is useful if
there is a lot of background.

[Minimum cell size]

Minimum cell size is used to determine the low range of cell size to include
in the measurement. The algorithm rst identies all objects, and calcu­lates the average size (e.g., 15 µm). From the percent of average size set­ting, the algorithm calculates the smallest object size to include in the nal
measurement (e.g., 70% of 15 is 10.5 µm; 15–10.5 = 4.5 µm; 4.5
µm would be the smallest particle included in the count). Adjusting the
number up, increases inclusiveness thereby decreasing the lower cell size
range (e.g., 50% of 15 is 7.5 µm; 15–7.5 = 7.5 µm).

Maximum cell size is used to determine the high range of cell size to in­clude in the measurement. The algorithm rst identies all objects, then
calculates the average size (e.g., 15 µm). From the percent of average size
setting, the algorithm calculates the largest object size to include in the
nal measurement (e.g., 200%; 200% of 15 µm = 30 µm; 30 µm is the largest
cell size included in the measurement.

Circularity is used to determine the objects to include in the measurement
based on roundness. Increasing the value from 80% requires objects to be
more round for inclusion in the measurement. Decreasing the value from
80% allows objects to be less round. Adjusting this may be useful if the cell
type is not particularly circular or perhaps oddly shaped.

4Ch

After modifying any parameters,
press APPLY button to make the changes.

To restore default parameters, press DEFAULT button.

3. Press NEW button to create a new protocol.

10

Setting

Parameter

4. Type protocol name and user name in the appropriate elds, and press
SAVE button again.

5. Once a protocol is saved, it is available for use at
any time. Press LOAD button.

6. The protocol appears in the protocol menu.
Use the up and down arrows to nd your saved protocol.
To use the protocol, select one of protocol and press APPLY button.

» Press DELETE button to delete protocol.

Press EDIT button to edit protocol.

Press CLOSE button to exit the screen.

EVE™ PLUS, Automated Cell Counter 11