NanoEnTek ADAM-MC User Manual

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ADAM-

ADAM

A New Standard of

A New Standard of Automatic Cell Counter

Automatic Cell Counter

INSTRUCTION MANUAL

USER MANUAL

NESMU-AMC-001E (V.5.5)

NESMU-AMC-001E (V.5.1)

Table of Contents

Product Contents Safety Information Product Specifications Description of ADAM-MC

Introduction

Overview Basic principals of counting

Getting Started

Environmental requirements Installation Start-up screen Error message during booting Menu setting System information Chip selection Counting method

General Operation

Introduction Preparing cell

Counting cell

Operating ADAM-MC Icon function Result analysis Error code Maintenance and cleaning

3 4 ~ 6 7 8 ~ 9

10 ~ 11

10 11

12 ~ 18

12 13 14 15 16 16 17 18

19 ~ 24

19 19

20 21 22 23 24

Software Installation

Introduction Getting started Installation Function guide Function buttons Data list Information Graph Sample image of error message

Trouble shooting Warranty Product list

Purchaser notification

25 ~ 32

25 25 26 ~ 27 28 29 30 30 31 32

33 34 35

Section 1

Product Contents

ADAM-MC

Accuchip kit

The contents of the ADAM-MC are listed below:

Item Quantity

Main device 1

User manual 1

USB cable 1

Installation CD 1

Key pad 1

Power cord 1

Fuse 2

External video monitor (Optional) 1

The contents of the ADAM-MC‟s Accuchip Kit are listed below:

Accustain

Item

Accuchip2x Kit

(Cat. No: AD2K-200)

Accuchip4x Kit

(Cat. No: AD4K-200)

Solution

(Cat. No:

ADR-1000)

Upon receiving the instrument

Disposable

Chip

Solution T 2 x 12.5 ml 2 x 12.5 ml 4 x 12.5 ml

Solution N 1 x 12.5 ml 1 x 12.5 ml 2 x 12.5 ml

Available

test Q‟ty

• Examine the instrument carefully for any damage incurred during transit.

• Ensure that all parts of the instrument including accessories listed above are included with the product.

• Any damage claims must be filed with the carrier.

• The warranty does not cover in-transit damage.

• See the 13 page to install the instrument.

• Upon receipt, store Accustain solution at room temperature.

200pcs (2 channel) 200pcs (4 channel) N/A

Min. 200 test/kit Min. 400 test/kit

Max. 400 test/kit

(Only total cell count)

Max. 800 test/kit

(Only total cell count)

Section 3

Safety

precautions

Safety Information

Always ensure that the power supply input voltage match the voltage

available in your location.

For operation environment, See page 12.

This machine is air-cooled so its surfaces become hot during operation. When installing it, leave a spaces of more than 10 cm (4 inches) around it.

Never insert metallic objects into the air vents of the instrument as this could result in electrical shock, personal injury and equipment damage.

Always set the main switch on the power supply unit to off before connecting the power cord to the wall outlet.

Always ensure that the grounding terminal of the instrument and that of the wall outlet are properly connected. The power cord should be connected to a grounded, 3-conductor power outlet.

To avoid potential shock hazard, make sure that the power cord is properly

grounded.

Do not position the equipment so that it is difficult to operate the disconnecting device.

Be sure to set the main switch to off, unplug the power cord and lock the stage before moving.

10.

If the instrument is broken or dropped, disconnect the cord and contact a authorized service person. Do not disassemble the instrument.

11.

Use only authorized accessories.

12.

Use this equipment only as specified in this manual and as specified in any documentation associated with its components. Any use of the equipment in an unspecified manner is strongly discouraged and may result in damage or injury as cautioned by signed warnings.

Section 3

Safety Information, continued

Safety

symbols

The symbols used in the ADAM-MC and the manual are explained below:

The Caution symbol denotes a risk of safety hazard.

ON (Power)

Protective earth (Ground)

The CE mark symbolizes that the product conforms to all applicable European

Community provisions for which this marking is required. Operation of the ADAM-MC is subject to the conditions described in this manual. The protection provided by the device may be impaired if the instrument is used in a manner not specified by the manufacturer.

Caution, Biohazard

Protective measures must be used in dealing with biologically hazardous

materials such as carcinogenic reagents.

Disposal of your old appliance

1. When this crossed-out wheeled bin symbol is attached to a product it means the product is covered by the European Directive 2012/19/EU.

2. All electrical and electronic products should be disposed of separately from the municipal waste stream via designated collection facilities appointed by the government or the local authorities.

3. The correct disposal of your old appliance will help prevent potential

negative consequences for the environment and human health.

4. For more detailed information about disposal of your old appliance, please contact your city office, waste disposal service or visit our web-site,

www.nanoentek.com.

Safety Information, continued

Warnings

Battery inside device

• Risk of explosion if battery is replaced by an incorrect type.

• This battery is not replaceable by a user.

• Refer to a qualified personnel.

Cover

• Do not remove a cover or dissemble a case.

• There is no adjustable components inside the instrument.

• If malfunction is found, refer to a service personnel.

Manual

• Do not attempt to service the equipment unless this manual has been consulted and is understood.

• This manual is available in English only.

• Failure to heed this warning may result in injury to

service provider, operator from electric shock, mechanical or other hazards.

Sample handling

• Wear gloves during sampling. User‟s sample may

have the infectious biohazardous substance.

Waste

• After using Accuchips, appropriately dispose it as biohazardous waste.

• Do not reuse the Accuchips.

Operator

• Must have the general knowledge of cell counting procedure and bio safety to handle the sample that may have the infectious biohazardous substance.

Section 1

Product Specifications

ADAM-MC

AccuChip

AccuChip 2x AccuChip 4x

Voltage AC 100 ~ 240 V,

50 ~ 60 Hz

Current max. 1.8 A, max 100 W

Fuse F3.15AL250V

Objective lens 4 X

LED Green LED

CCD camera B/W CCD

Filter Excitation filter

Dichroic filter

Emission filter

Weight 9Kg

Size (W×L×H) 220 × 375 × 250 mm

Degree of protection

Measuring range

IPX0

5ⅹ104~ 4ⅹ106cells/mL

AccuStain

solution T,N

Accessories

Analysis time 45 sec. ~ 2 min./test

Loading sample

vol. per test

Measuring

sample vol. per test

20 µL/test (AccuChip 2X)

12 µ L/test (AccuChip 4X)

8.5 µ L/test (AccuChip 2X)

3 µ L/test (AccuChip 4X)

PI (Propidium Iodide) staining of

total cells (T) and non-viable cells (N).

Power cord 1.5 m

Fuse 250 VAC, 3 A;

F3.15AL250V

Keypad

USB Type / 1.3 m

Section 1

Description of ADAM-MC

Front view of ADAM-MC

The ADAM-MC, an automated cell counter, is a benchtop automated cell counter that performs cell count and viability measurements using Accustain solution.

The front view showing various parts of the ADAM-MC is shown below:

Control Button

Door

LCD

• Control buttons:

Eject Ejects the chip holder from the ADAM-MC.

Start Performs all procedures of automatic counting.

Lock Protects the alignment of stage from external shock

☞ It is strongly recommended to lock ADAM-MC before turning it off.

• Door

• LCD

• keypad

Key Pad

when the ADAM-MC is moved to the other places.

Chip holder comes out here.

Displays the process and the result.

Inputs the sample number less than 3 characters and press „Enter‟ button.

Section 1

Description of ADAM-MC, continued

Rear view

of ADAM-MC

The rear view showing various parts of the ADAM-MC.

Fan

Key Pad Port

Power Switch

RS232C Port

Fan Cooling fan

Power switch Main power on/off switch

Power plug ADAM-MC power cord connection plug

USB port Connect to computer with USB Cable

RS232C port Not connect (Port for only QC and Service)

Key pad port Keypad connection port

Video Port External video monitor port

USB Port

Video Port

Power Plug

Introduction

Overview

Until now, cell counting and viability measurement for many types of cells have been performed manually using hemocytometer with Trypan Blue exclusion method, which is to distinguish viable cells from non-viable cells. One drawback of this method, however, is the propensity for the staining of artifacts; another drawback is that the naked eye can only differentiate between cells in a limited concentration range in the hemocytometer chamber. This combined with the potential problem of cell aggregation and limited sample volume leads to the common variation of counts normally associated with this method. To address these problems, NanoEnTek has developed the ADAM-MC, which is based on a fluorescent microscopy technique for counting cells. The ADAM-MC utilizes sensitive fluorescence dye staining, LED optics and CCD detection

technologies to make the cell analysis more accurate and reliable.

To count cells using ADAM-MC, the cells are mixed with a Propidium Iodide (PI) stain and directly pipetted on to a disposable plastic chip. The chip is then loaded onto a precision stage. An ADAM-MC system is automatically focused onto the chip and cells that have been stained are recorded by a sensitive CCD camera. The image results are automatically processed generating the cell count which is displayed on the front of the instrument. Simple. Fast. Accurate. Reliable.

Introduction, continued

Basic principals of counting

ADAM-MC is based on staining mammalian cell DNA with a fluorescent dye, Propidium Iodide (PI). PI does not enter cells with intact cell membranes or active metabolism. In contrast, cells with damaged membranes or with inactive metabolism are unable to prevent PI entering the cell. As a result, the nuclei of non-viable cells will only be stained. The ADAM-MC provides two

kinds of staining solutions. AccuStain Solution T for the total cell counting is

composed of the fluorescent dye (PI) and lysis solution. AccuStain Solution N for the non-viable cell counting is composed of the fluorescent dye and PBS. In order to measure the total concentration of cells, the plasma membranes of all the cells must be disrupted to stain all the Nuclei with PI. The process of disrupting and staining is achieved by treatment with AccuStain Solution T. In the second solution, live cells remain intact and are not stained. Only the non-viable cells are stained and detected. After treatment, the prepared

cells will be loaded into the chip. The viability will be automatically calculated in the ADAM-MC software after each measurement of the total cells and the non-

viable cells.

 A: Total cell  B: Non-viable cell

Getting Started

Environmental requirements

To obtain the best results, install the ADAM-MC in a location following conditions:

1. Room temperature between 20 and 35 °C.

• Not recommended for cold room use (≤ 4 °C).

• At low temperature (≤10 °C), warm up the ADAM-MC for 10 min.

2. Not exposed to direct sun light.

3. Not subject to direct or continuous vibration..

4. Not subject to intense magnetic or electromagnetic fields.

5. Relative humidity between 0–95 %.

6. Area free from corrosive gases or other corrosive substances.

7. Area with very little dust or other airborne particles.

8. Allow a 10 cm minimum space around the instrument

for proper air flow.

9. Not allow to put heavy material on top of ADAM-MC.

Section 3

Getting Started, continued

Installation

1. Remove all components of ADAM-

MC from their box.

2. Place the instrument in a flat level and dry surface.

3 Plug to power cord into the

electrical outlet.

• Be sure to use only the

power cord supplied with your instrument.

• Powering the instrument with

an unapproved power cord

may damaged the instrument.

4. Plug to keypad.

5. turn on the power switch.

• Make sure that the main

power switch is In the “Ⅰ” (ON) position.

Section 3

Getting Started, continued

Start-Up

screen

1. System booting.

2. System will go through self diagnostic tests.

• If you get an error message,

please contact your local distributor or

sales@nanoentek.com.

3. The home screens will be displayed as the image, no errors are detected.

Section 3

Error

message during booting

Getting Started, continued

It appears when booting not

working properly.

Turn off main power and

restart device.

If this message still appears after restart, contact your local distributor or

sales@nanoentek.com.

It appears when a chip is inserted during Start up.

Remove the chip from a device, and Do not turn on the device with a chip.

If this message continues to display when no chip is inserted, contact your local distributor or

sales@nanoentek.com.

1.2.It appears when turning off

without locking device.

Push the lock button before turn

off the device. If this message still appears after restart, contact your local distributor or

sales@nanoentek.com.

Section 3

Getting Started, continued

Menu setting

System information

1.2.You can set the menu as you

press the „*‟ button on the keypad

from the screen for inputting sample numbers.

You can select the number from the Menu.

Check the device versions and date which have been installed in the device.

[System Info.]

1. Version : MC V1.21

2. Date : Sep 23, 11 CORE1 : 1.87 CORE2 : 1.52 CORE3 : 1.53

Select number 4 key from the

MENU, and Press the „Enter‟ key.

The screen will return to the Menu screen automatically.

Section 3

Getting Started, continued

Chip

selection

1. Press „1‟ for chip selection.

You can select the number from the Menu.

2.3.User can select the 2 kinds of chip type.

• „1‟ : Two channel chip

(AccuChip2X)

• „2‟ : Four channel chip

(AccuChip4X).

Press „1‟ or „2‟, and Enter key.

4. After pressing the „Enter‟ key, the screen will return to the Menu screen automatically.

Section 3

Getting Started, continued

Counting method

1. Press „2‟ for counting method. You can change the capture frame mode.

2. You can select the 60 or 30 frames in 2CH mode and 22 or 11 frames in 4CH mode for counting as well.

2CH High Precision: 60 frames capture High Speed : 30 frames capture

4CH High Precision: 22 frames capture High Speed : 11 frames capture

3.4.Press „1‟ or „2‟ and „Enter‟ key.

After pressing the „Enter‟ key,

the screen will return to the Menu screen automatically.

Section 4

General Operation

Introduction

Preparing cell

Counting cell

Instruction is provided in this section for preparing the

cell sample with Accustain solution for use with disposable Accuchip for automated cell count using the ADAM-MC.

1) Cultivate the required number of cells.

2) Add an appropriate volume of growth media or PBS to dilute

to a final concentration of 5ⅹ104cells/ml to 4ⅹ106cells/ml.

Note☞Concentration out of this range will result in errors.

Refer to page 23 for more information about errors.

3) Thoroughly mix the cell pellet by vortexing.

4) Check visually if any cell clumps or agglomerates are remained.

 Accuchip 2x

Non-viable

Total

Total Cell Non-viable Cell

1) Add 50 µL of your sample to 50 µ L

supplied Accustain solution T.

2) Mix gently by pipetting up and down.

3) Load 20 µ L sample mixture to the

Accuchip on T channel.

1) Add 50 µL of your sample to 50 µ L

supplied Accustain solution N.

2) Mix gently by pipetting up and down.

3) Load 20 µ L sample mixture to the

Accuchip on N channel.

 Accuchip 4x

Non-viable

Total

Total Cell Non-viable Cell

1) Add 50 µ L of your sample to 50 µ L

supplied Accustain solution T.

2) Mix gently by pipetting up and

down.

3) Load 12 µL sample mixture to the

Accuchip on T1 or T2 channel.

Note☞When you load of the sample mixture to the Accuchip, please be careful not to make bubbles.

1) Add 50 µL of your sample to 50 µ L

supplied Accustain solution N.

2) Mix gently by pipetting up and down.

3) Load 12 µ L sample mixture to the

Accuchip on N1 or N2 channel.

Non-viable

Total

Section 4

Operating ADAM-MC

General Operation, continued

Press “ Eject(▲)” button on the main device to eject the chip holder.

2. Insert the Chip loaded with the sample onto the chip holder.

Press the “Start(▶) ” button on the main device.

Note☞Automatic Focus will be carried out at the first time the device is booted.

Once ADAM-MC has done the Auto Focus process and on the following time,

focusing process will be skipped.

4. The instrument takes approximately 90 seconds to count sample.

5. After calculating the cell number, the chip will be ejected

automatically.

Then chip can be removed.

6. The calculated cell number per

ID1 : 123 ID2 : 456 T1=1.10E6 N1=5.50E5 T2=2.20E6 N2=5.50E5 T1-N1=5.50E5 V1= 50% T2-N2=1.65E6 V2= 75%

1ml will be displayed on the screen automatically.

7. For another experiment, repeat the process from steps 1 ~ 5.

Section 3

Icon

function

General Operation, continued

ID1 : 123 ID2 : 456 T1=1.10E6 N1=5.50E5 T2=2.20E6 N2=5.50E5 T1-N1=5.50E5 V1= 50% T2-N2=1.65E6 V2= 75%

1. Display a status of the performance such as Start, Eject, Lock or Insert.

Eject

Start

Lock

Insert

2. Display menu setting

Shows the Chip Holder is ejected . (After pressing the Start button).

Shows when cell counting is running. (After pressing the Eject button).

Shows the Chip Holder is parked . (After pressing the Lock button).

Shows the Chip Holder is inserted. (After pressing the Eject button with AccuChip).

Shows that performance setting is high precision. ADAM-MC scans 22 fields in each chamber, repres enting a total volume of 3.1 µL.

Shows that ADAM-MC reads 4 Channel chip.

Shows that ADAM-MC reads 2 Channel chip.

3. Display error message

LHOU E E

Error code.

( go to page 23. )

Section 4

General Operation, continued

Result analysis

Press the „*‟ key after menu setting. Once inputted, the screen will return to the

counting mode automatically.

ID1 : 123 ID2 : 456 T1=1.10E6 N1=5.50E5 T2=2.20E6 N2=5.50E5 T1-N1=5.50E5 V1= 50% T2-N2=1.65E6 V2= 75%

* 1.10E6 = 1.10 X 106cells/ml

Sample

name

ID1 123 T1 (1.10E6) N1 (5.50E5) 50%

ID2 456 T2 (2.20E6) N2 (5.50E5) 75%

•The viability will be automatically calculated by the ADAM-MC software after each measurement of the total cells and the non-viable cells.

Number of

Total cell

Number of

Non-Viable cell

Viability

•First, the total cell number and second, non-viable cell number were

measured and then the cell viability is calculated as subtracting non-viable cell counting numbers from total cell counting.

ID 1

AccuChip 2x

ID 2ID 1

AccuChip 4x

Section 4

Error code

General Operation, continued

Check a chip is inside of device when turning on the device.

ID1 : 123 ID2 : 456 T1= error N1= error T2= error N2= error T1-N1= error V1= 0% T2-N2= error V2= 0%

ID1 : 123 ID2 : 456 T1= 3.99E5 N1= 2.01E6 T2= 4.01E6 N2= 4.99E4 T1-N1= 1.61E6 V1= 80% T2-N2= 3.96E6 V2= 98%

Removing the chip first, then rebooting the device.

If this message come up again after removing a chip, contact your local distributor or

sales@nanoentek.com.

Frames with errors are over 50% of total counting frame. Frame with error is a frame that contains cells whose diameter is larger than 30m.

Cells are more than 4X10 cells/ml. “Over detection range” will be shown on Report program.

[ 2 channel ]

[ 4 channel ]

Cells are more than 2X106 cells/ml.

“High concentration cells” will be

shown on Report program.

Cells are less than 4X105cells/ml.

“Low concentration cells” will be

shown on Report program.

Cells are less than 5X104 cells/ml.

“Under detection range” will be

shown on Report program.

General Operation, continued

Maintenance

and cleaning

1. ADAM-MC does not need regular maintenance.

2. ADAM-MC has no replacement of consumable materials.

3. Clean the exposed outer surface of ADAM-MC using a soft cloth and

isopropyl alcohol or deionizers water.

CAUTION:

Dispose of wipes in an appropriately labeled solvent

contaminated waste container.

Section 5

Software Installation

Introduction

Getting started

ADAM-MC Software Program is designed to manage and report all results

from ADAM-MC.

• All measurement results are saved on the memory of ADAM-MC,

automatically.

• User can download the data from the memory of ADAM-MC and export it to

Excel (*.xls) format.

• User can delete data from memory of ADAM-MC or can save captured

images into Desktop or Laptop hard drive.

• The data list window consists of the sample number, chip, date, time, total,

nonviable, viable, viability counting result in %.

※CAUTION:

Before running the program, check the connection of USB cable between the ADAM-MC and the laptop or desktop computer.

The following steps are guide for connecting USB cable:

1. Connect the USB cable to ADAM-MC.

2. Connect the USB cable to desktop or laptop computer.

3. Turn on ADAM-MC and Desktop computer.

Section 5

Software Installation, continued

Installation

To install the ADAM-MC Operation software, follow the directions as below:

Insert the installation CD-ROM into the computer. Then open the file

“Setup_ADAM_v1.x.x.x.exe”.

※Report program can be

installed in Windows 2000, XP or higher version.

The start-up dialogue of the software, as shown like left image, will appear.

Click “Next” to start

installation.

If you want to change installation folder, click

“Browse” and choose the

location that you want.

Section 5

Installation

Software Installation, continued

After choosing installation

folder, click “Install” to

proceed with the installation.

The computer activates the

“Installation of the Software”.

☞

Initial installation folder is

“C:\Program Files\Digital

Bio\ADAM”.

Report Program will be installed automatically.

Click “Ok” to finish the

installation.

If the installation was successful, the report program can be found at Start>All Program>ADAM.

Function guide

Software Installation, continued

⑤

① Image frame

①

②

③ ④

Image captured by ADAM-MC will be shown here

② Function

Buttons

③ Data List

④ Information

⑤ Graph

Start cell counting, saving images, exporting data, and all function of Report Program are handled by using these buttons. (see p.29 for more detailed information of each button)

All saved data in ADAM-MC will be loaded and shown in data list section.

Operation and counting results of each frame will be displayed here.

Analysis of results including cell size and frame by frame counting will be shown in graph section.

Section 6

Software Installation, continued

Function

buttons

Start cell counting.

Park (Lock) stage of ADAM-MC.

Eject chip holder out of ADAM-MC.

Loads the experiment data from the memory of the main device.

Deletes all of the loaded data and memory of the main device.

Transfers the data list to Excel format and saves it.

Turn on or off automatic image save option.

Default image save folder is “C:\Program Files\Digital Bio\ADAM\Images”. Images will be saved until your hard drive has no more capacity to save. Be sure to set Auto Save off, unless you need to save images. Example of saved image file: 081221(yymmdd)203452(hhmmss)-N1(channel name)-002.bmp

Choose folder to save images automatically.

Data list

Software Installation, continued

Data list shows data stored in ADAM-MC memory.

Total amount of stored results are indicated at bottom of list as “Saved experiment”.

Up to 200 counting results are automatically saved to ADAM-MC memory.

Information

When memory of ADAM-MC is full, new counting result will replace old data.

These data can be exported as Excel Sheet (*.xls) and stored in personal

computer or can be erased from ADAM-MC memory.

This section shows information regarding operation of ADAM-MC. If cell counting is started through Report Program, the counting results of each frame that ADAM-MC captures will be shown here.

Graph

Software Installation, continued

This section shows information of cell size distribution and counting results of each frame that ADAM-MC captured. Through cell size graph, you can figure out whether there are cell clumps or aggregates. In case of counting evenly distributed cells without any aggregation, there should be a single peak on distribution of cell size.

• The size of cell in graph is not real size of cell. It is the size of pixels in

fluorescence image captured by ADAM-MC. And the size information is only to judge if there is a lot of aggregated cells.

Vertical red line indicates the cut-off size. Counting results between first and second red line are counted as single cell and those between second and third line are counted as 2 cell, and so on. Any results before first red line will not be counted as cell.

Sample

image of error message

Software Installation, continued

Trouble shootingAppendix A

Trouble shooting

Problem Cause Solution

ADAM-MC does

not power up

Inaccurate result

Software does not work

• Power switch in off position

• No power from outlet

• Bad power cord

• Cell number may be out of

range.

• AccuStain Solution has expired.

• Too high clumped cells

• PC setup incorrect/wrong

instruct mode.

• Cable's not fully connected/ wrong adaptor.

Check power switch on back of unit.

Check power source.

Replace.

Adjust the number of

cells between 5X104~

4X106cells/ml (refer to page 19).

Check the expired date.

Try again after vortexing the cells.

1.2.Check program setup.

Check all connections.

When error message is shown (☞ For information on each error

message,

see page 23.)

• When there are too many frames with errors (Error message: E)

• High concentration of cells

(Error message: H)

• Over detection range (Error message: O)

• Low concentration of cells (Error message: L)

• Under detection range (Error message: U)

1.2.Check the suspension of cells if all cells are fully dissociated into single cells.

If contaminants except

cells are found, prepare

sample again.

1.2.Check if concentration

of cell is too high.

Dilute the sample and count again.

1.2.Check if concentration of cell is too low.

Use concentrated sample and count again.

Warranty

NanoEnTek warrants that the ADAM-MC will be free from defects in material and

workmanship for a period of one (1) year from date of purchase.

If any defects occur in the ADAM-MC during this warranty period, NanoEnTek will repair or replace the defective parts at its discretion without charge.

The following defects, however, are specifically excluded:

• Defects caused by improper operation.

• Repair or modification done by anyone other than NanoEnTek or

an authorized agent.

• Damage caused by substituting alternative parts.

• Use of fittings or spare parts supplied by anyone other than

NanoEnTek.

• Damage caused by accident or misuse.

• Damage caused by disaster.

• Corrosion caused by improper solvent or sample.

For your protection, items being returned must be insured against possible damage or loss.

NanoEnTek cannot be responsible for damage incurred during shipment of a repair instrument; It is recommend that you save the original packing material in which the instrument was shipped.

This warranty should be limited to the replacement of defective products.

For any inquiry or request for repair service, contact sales@nanoentek.com or your local distributor.

Appendix D

Product List

Additional product list

Cat. No. Product Contents Quantity

ADM-001

AD2K-200 AccuChip

AD4K-200 AccuChip

External video monitor (optional)

Kit

7” LCD Monitor 1

200 pcs AccuChip

12.5 ml AccuStain solution T

12.5 ml AccuStain

solution N

200 pcs AccuChip

12.5 ml AccuStain solution T

ADR-1000 AccuStain

12.5 ml AccuStain solution N

12.5 ml AccuStain solution T

12.5 ml AccuStain

solution N

Appendix D

All the materials in this manual are protected by Korean and international copyright laws. They cannot be reproduced, translated, published or distributed without the permission of the copyright owner.

ADAM-MC, User Manual

Website : www.nanoentek.com E-mail : sales@nanoentek.com

Manufactured by

NanoEnTek Inc. (HQ)

12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 152-740, Korea Tel :+82-2-6220-7940 Fax:+82-2-6220-7721

The information in this manual is described as correctly as possible and is

applicable to the latest firmware and software versions, but it may be

changed without prior consent or notification.

Documentation : NESMU-AMC-001E (V.5.1)

Revision History :

V.0.0

V.1.0

V.1.5

V.2.0

V.3.0

V.3.5

V.4.0

V.5.0

V.5.1

October 2008

February 2009

June 2009

September 2009

January 2010

November 2010

February 2012

April 2012

March 2014

Contact Information

E-Mail : sales@nanoentek.com

Website : www.nanoentek.com

Developed and Manufactured by NanoEnTek Inc.

NanoEnTek Inc. (HQ)

12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 152-740, Korea Tel :+82-2-6220-7940 Fax:+82-2-6220-7721

NanoEnTek USA Inc.

5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA Tel :+1-925-225-0108, +1-888-988-0108(Toll free) Fax:+1-925-225-0109

NanoEnTek ADAM-MC User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual