Nanoentek Adamii User Manual

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Bench-top Fluorescence Cell Counter

User manual

All the materials in this user manual are protected by Korean and international copyright laws.

They cannot be reproduced, translated, published or distributed without the permission of the

Every effort has been made to ensure that all the information contained in the ADAMII™ User

Manual is correct at the time of printing. However, NanoEntek Inc. reserves the right to make any

changes necessary without notice as part of ongoing product development.

ADAMII™ User Manual

Website: www.nanoentek.com

E-mail: ivdst@nanoentek.com

Documentation: NESMU-ACD-001E(V.0.0)

Revision history: V.0.0 Date: March 2017

Table of contents

Introduction

Product components

Product description

Installation

Home menu

Calibration

Sample loading

Operation

- Insert assay slide

- Measure menu

· Initial mode

· Focusing mode

- Data menu

- Setting menu

Cleaning & Maintenance

Troubleshooting

Error code

Warranty

Safety precautions

Safety symbols

Product specications

Ordering information

Technical support

6-7

8-9

10-11

12-19

21-49

21-23

24-33

24-26

27-33

34-43

44-49

51-52

53-54

56-57

Introduction

ADAMIITM is a bench top uorescent cell counting analyzer that can be used in either a hospital and

research laboratory.

It has 4-channel (Bright, Yellow (PE), Red (PerCP) and Green (FITC)) light sources, so three color

uorescence samples can be analyzed simultaneously. The automatic X-Y-Z stage captures a maxi-

mum of 136 images and which increases the accuracy of analysis.

The obtained images are processed by image analysis software integrated in the system. All proce-

dures are automatic once the stained sample has been placed in the disposable assay slide.

[Features]

• A benchtop uorescence cell counting analyzer

• Can be used in hospital and research laboratory

• Visualize: Quantitative results with visual conrmation

• Quantify uorescence-labelled cells without sheath tank maintenance

Product components

ADAMII™ is shipped with the following components. Once you receive your instrument, please

check that all components listed below were shipped.

If any component is missing or damaged, contact your local distributor or e-mail ivdst@nanoentek.com

ADAMII™

1 EA

User manual

1 EA

Power cord with 4 adaptor cords

(for U.S./Canada/ Taiwan/Japan,

Europe or UK)

4 pcs/1 SET

Focus guide

1 EA

USB cable

2 EA

Operating laptop

(Please note: Laptop will be packaged separately)

1 set

Product description

Front view

Side view

Insert/Eject

button

Run button

State LED

Slide Holder

Door

Insert/Eject Button

Run Button

State LED

Insert and eject the slide from the ADAMII™.

Functions as load and unload.

Perform all procedures of automatic counting.

Light up when software runs in the laptop and

connection of camera and serial perform cor-

rectly.

Focus Knob

Slide Holder

Door

Focus Knob

Location to insert or remove the slide.

Open to check the slide holder.

Adjust focus to obtain better cell images.

Product description

Rear view

Power

supply port

Power supply port

Power switch

Serial connection port

Camera connection port

Power switch Serial

Serial Camera

connection port

Power supply

Power on/off

Control the ADAMII™ operating

Control the camera of the ADAMII™

Camera connection port

Installation

Unpacking the instrument

Install the instrument

1. Open the box and remove the material foam in the box.

2. Carefully lift the instrument out of the box.

3. Place the instrument on a at, level surface.

1. Plug the power cord into the electrical outlet and connect two USB cables..

Power supply port USB port

Serial Camera

2. Connect the laptop and the ADAMII™ using two USB cables.

Installation

Install SW program

1. Turn on the laptop and ADAMII™ by pressing the Power button. At

this time, the slide holder will be ejected automatically.

2. Double-click ADAMII™ icon on Desktop to start the software.

☞

Note: The ADAMII™ will be ready to use after initialization is

completed when powered on.

☞

Note: When the software runs and is connected with ADAMII™

normally, the state LED of instrument will be lighted up.

CAUTION:

• You must turn on the ADAMII™ before running the software program.

Home menu

User management

At the ‘Home’ screen, enter the user ID and password to log in.

Default ID and password are both ‘master’.

Click ‘User management’ when you need to manage accounts (e.g. ID

addition, password change). To create a new ID, ll out the blank (user

name, user ID, password, conrm PW) and click ‘Add’.

☞

Note: Only the master user can access the ‘User management’ .

Home menu

Audit trail

Click ‘‘Audit trail” to search and trace detailed information of each user.

1. Enter the User ID and set the date that you want to search.

2. Click ‘Search’ button.

3. Information of the selected user will appear.

☞

Note: Only the master user can access the ‘‘Audit trail”.

Calibration

Calibration procedure

1. For calibration, go to ‘Setting’ menu and click ‘Calibrate’.

2. Then, calibration window will appear.

Calibration

Calibration procedure

3. Shake bottle vigorously or vortex briey for 5 seconds before use.

5 sec.

[Shake bottle vigorously]

4. Once again, vortex for 4 seconds and pipette for 25 μL immediately.

4 sec.

[Vortexing]

[Pipetting calibration bead 25 μL]

[Vortexing]

5. Load 25 μL of calibration beads on the ADAMII™ assay slide.

[Load 25 μL calibration bead]

Calibration

Calibration procedure

6. Attach dry-proof closure tape to prevent drying.

[Attach dry-proof closure tape]

Warning:

• Calibration bead loading error

- Ensure that 25μL of calibration bead is fully loaded on the ADAMII™

assay slide.

- The instrument will not detect in low or high loading volumes.

CAUTION:

• Avoid bubbles as theymay negativelyaffect results.

Low volume Correct volume

• Boundary between black and white on the tape should be lined up

with the edge of the slide.

Dry-proof closure tape

Calibration

Calibration procedure

7. Leave for 20 seconds (need time for beads to settle).

20 sec.

8. Insert the slide with calibration beads into the slide port of the

instrument and press ‘Insert sample’ button.

9. Enter lot number of calibration beads.

10. Select Bright channel and adjust the focus using the focus knob.

Calibration

Calibration procedure

☞

Note: Please check ‘ADAMII™ Focus guide’ before setting the focus.

[Focusing example - representative images]

Bright- Good Focus

Good Focus VS. Bad Focus

FL- Good Focus

• Bright - Good Focus: Clear circumference of the beads, Dark and

clear contrast to the background

• Bright - Bad Focus: Blurry and unclear bead circumference

• FL - Good Focus: Bright uorescence beads with clear contrast to the

background

• FL - Bad Focus: Weak uorescence beads with unclear contrast to the

background

Bright- Bad Focus

FL- Bad Focus

Calibration

Calibration procedure

11. Press the ‘Save BR Focus’ after adjusting focus.

12. Select PerCP channel and adjust the focus using the focus knob.

13. Press the ‘Save FL Focus’ after adjusting focus. The focus of other

uorescence channels will be set automatically.

☞

Note: Save the focus in ‘Bright’ and ‘PerCP’ channel.

The other channels will be automatically focused.

14. Press the ‘Run’ button to start the auto calibration. Calibration will

take about 3 minutes to count the 24 frames.

3 min.

Calibration

Calibration procedure

15. When the calibration is completed, the results will be displayed.

☞

Note: If the results are not within the range on the tube,

please ask your manufacturer or distributor.

16. When you need previous results of calibration, click ‘Record’ button.

Calibration

Calibration procedure

17. Calibration result list records will appear.

18. Click ‘Save’ button to save the calibration records. The result will be

saved in CSV format.

19. Press “Close” button to nish calibration.

Sample loading

Sample preparation

Load sample

Prepare sample and check the sample type.

To run a sample, click ‘Measure’ menu.

Load 25 μL sample into the ADAMII™ assay slide and attach a dry-

proof closure tape.

[Load 25 μL sample] [Attach dry-proof closure tape]

Note: Please refer to page 14 to check cautions for loading correct

☞

volume and attaching the dry-proof closure tape.

☞

Note: Note: Before loading the antibody stained specimen or bead to

the slide, vortex enough to mix them up.

Operation

Insert assay slide

1. Open the ADAMII™ door.

2. Press to open up the slide holder.

3. Insert the assay slide into the slide holder.

☞

Note: Assay slide should be inserted to the end of the slide holder, in the direction of arrow on the slide, facing up. Incomplete insertion of slide may cause erroneous results.

4. Close the slide holder cap tightly.

Warning:

• If you don’t close the slide holder cap, the slide will not be inserted.

Operation

Insert assay slide

Warning:

• ADAMII™ assay slide insert error

- Completely insert ADAMII™ assay slide face up, in the direction

of the arrow on the slide. The instrument will not detect if slides

are inserted incorrectly. Illustrations of various possible cases of

erroneous assay slide insertion are presented below.

Correctly inserted

( O )

Not fully inserted

( X )

( X ) ( X )

Upside down inserted

Adverse direction inserted

(Arrow mark)

Warning:

• Using ADAMII™ assay slide

- Do not reuse the ADAMII™ assay slide, as leftover dye from the

previous reading may affect the next reading.

- Do not use any other slides as it results in inaccurate count and

may damage the instrument.

Operation

Insert assay slide

5. Close the door of the ADAMII™.

6. Select the type of sample to run.

7. Press the ‘Insert/Eject’ button on top of the instrument, or “Insert

sample” button on the screen. The slide will automatically be moved

into the instrument.

☞

Note: LED light of the ‘Insert/Eject’ button shows the status of the

slide holder.

· Slide inserted – Off

· Slide ejected – On

· Slide inserting and ejecting - blinking

Warning:

• Close the door after inserting the sample.

• Do not look into the ADAMII™ instrument when door is open.

Direct exposure to high-energy LED light may be harmful to the eyes.

Operation

Measure menu

- Initial mode

To do a sample test, click the ‘Measure’ menu.

1 4

☞

Note: Above screen and upcoming screen images only relate to CD34

application.

1. Counting mode

2. Display area

3. Detection channel

4. Insert sample

5. Result area

6. Test information

Operation

Measure menu

- initial mode

① Counting mode

② Display area

Select the type of sample to measure.

• Sample type: HPC-A, Mobilized PB, Control

• Dilution factor: Enter the dilution factor when the diluted sample

was stained and to be measured.

• Frames: Number of images to be captured. Recommended

number of frames is 136. Depending on cell density,

adjust the number of frames.

• Viability: Check the circle to include to get viability data.

Captured image of sample will be displayed. It will be activated after

inserting sample. (Refer to page 28)

③ Detection channel

④ Insert sample

The selected channel will be displayed. It will be activated after

inserting sample. (Refer to page 28)

Insert the sample loaded assay slide and click ‘Insert sample’ button

to start the test.

☞

Note: Please check the door and slide holder cap is closed.

Operation

Measure menu

- initial mode

⑤ Result area

⑥ Test

information

Counting results of the sample will be displayed. It will be activated

after inserting sample. (Refer to page 33)

Date, sample/patient ID, and operator information will be displayed.

Operation

Measure menu

- Focusing mode

Once the assay slide is inserted, focusing mode will appear.

☞

Note: Above screen and upcoming screen images only relate to

CD34 application.

1. Display area

2. Detection channel

3. Focus option

4. Save focus

5. Run sample

6. Eject

Operation

Measure menu

- Focusing mode

① Display area

② Detection channel

Display preview image of the sample from the camera.

Indicate which channel is displayed. For ADAMII™-CD34 assay,

three uorescent channels correspond to the markers below:

• PerCP: CD45

• PE: CD34

• FITC: live/dead marker

☞

Note: FITC channel will be measured only when the viability circle is

selected.

③ Focus option

1. Focus can be adjusted manually or automatically. Focusing knob

is right side of the instrument.

Operation

Measure menu

- Focusing mode

③ Focus option

2. • Auto focus: set the focuses for all channels automatically,

when this option circle is selected.

• 2ch manual focus: Set the focuses for two channels ‘Bright’ and

‘PerCP’ manually, then the other two uorescent

channels will be automatically focused, when this

option circle is selected.

☞

Note: 2ch manual focus is recommended.

[Save BR focus]

[Save PerCP focus]

Operation

Measure menu

- Focusing mode

③ Focus option

☞

Note: Please check ‘ADAMII™ Focus guide’ before setting the focus.

[Focusing example – bright eld]

Bright- Good Focus

Good Focus VS. Bad Focus

Bright- Bad Focus #2

• Good Focus: Clear cell image – sharp cell membrane, dark cell body

with contrast to the bright background

• Bad focus #1: Unclear cell image – no contrast to the background

• Bad focus #2: Bright and white cell body undistinguishable to the background

• Bad focus #3: Blurry and unclear cell image

☞

Note: The example images only relate to CD34 application.

Bright- Bad Focus #1

Bright- Bad Focus #3

Operation

Measure menu

- Focusing mode

③ Focus option

☞

Note: Please check ‘ADAMII™ Focus guide’ before setting the focus.

[Focusing example – uorescence channel]

CD45 PerCP- Good Focus

Good Focus VS. Bad Focus

CD34 PE- Good Focus

Bright- Bad Focus #2 Bright- Bad Focus #3

FITC - Good Focus

• Good Focus: Clear cell image, bright cell body in contrast to the

dark background

• Bad focus: Unclear cell image, blurry cell image and donut shaped cell

image

☞

Note: The example images only relate to CD34 application.

CD45 PerCP- Bad Focus

CD34 PE- Bad Focus

FITC - Bad Focus

Operation

Measure menu

- Focusing mode

⑤ Run sample

1. Once the focusing is done, dilution factor and number of frames to

capture. If you want to see the viability, please check the circle

under the viability circle.

2. Press the “Run sample” button.

3. Enter the sample ID, patient ID, antibody lot number and slide

lot number.

4. Counting the 136 frames is recommended.

When the counting is done, assay slide will be ejected automatically.

15 min.

Operation

Measure menu

- Focusing mode

⑤ Run sample

⑥ Eject

5. The calculated data including total cell count and viability of CD34

and CD45 will be displayed in the data presenting area.

6. To run the next sample, take out the nished assay slide, insert

the new sample loaded assay slide, repeat focusing and running

procedure.

Click ‘Eject’ button when you want to manually eject the sample.

There is no need to use this button after the assay is ordinarily com-

pleted, when the sample will be ejected automatically.

☞

Note: The door should be closed when the slide holder is being ejected.

If not, the door can be damaged.

☞

Note: If the door is opened, the sample will not be ejected.

Operation

Data menu

Data menu provides the raw data including counting results, images

from each frame in each channel. Users are able to review, save and

export the data.

Data list

☞

Note: Above screen and upcoming screen images only relate to CD34 application.

1. Test information

2. Sample information

3. Captured image

4. Sample volume information

5. Result area

6. Histogram

7. Raw data table

8. Data list

9. Export data

10. Edit data list

Operation

Data menu

① Test information

② Sample information

Test information shows the date, sample/patient ID, and operator

information.

• Measured date: displays measured date and time

• Sample ID: displays sample ID

• Patient ID: displays patient ID

• Operator: displays operator’s ID

User can check the frame numbers and sample volumes of

captured images.

• Sample type: display the sample type

(HPC-A, Mobilized PB, Control)

• Reading frame/volume: total frames and volume

• Deleted frame/volume: deleted frames and volume

• Result frame/volume: nal result frames and volume

(Subtract the deleted frame/volume from

the reading frame/volume)

Operation

Data menu

③ Captured image

Image section displays the captured images. It is also available to

check the cells in each channel.

Press circle button of each channel to identify the cells counted

through a particular channel. ADAMII™ circles the cells that were

analyzed as follows.

• Yellow: cells counted in bright channel

• Purple: cells counted in PerCP channel (CD45 in ADAMII™-CD34 assay)

• Green: cells counted in PE channel (CD34 in ADAMII™-CD34 assay)

• Red: cells counted in FITC channel (dead cells in ADAMII™-CD34 assay)

☞

Note: This function is available only in the data menu.

Operation

Data menu

③ Captured image

Double click the captured image, then you can check preview images of

selected channel on the right side of the screen. To check the preview im-

ages of other channels, select that channel and double click the captured

image again.

Operation

Data menu

④ Sample volume information

It shows the total sample volume with RBC lysis buffer and original

sample volume used for staining.

The default numerical values of total and sample volumes for each

sample type in ADAMII™-CD34 assay are shown below:

• HPC-A: total volume = 275 μL, sample volume 20 μL

• Mobilized PB: total volume = 60 μL, sample volume 20 μL

• Control: total volume = 275 μL, sample volume 20 μL

Click ‘Edit’ button to edit the total volume, sample volume and dilu-

tion factor. If there are many cells in the sample, edit the dilution factor.

⑤ Result area

Result area displays reportable counting results of the selected data.

• Events: displays the event count

• %: displays the percentage of CD34 contrast to CD45

• Cells/μL: displays the number of cells per μL

Operation

Data menu

⑥ Histogram

User can check the cell size graph of each channel. Cell size gating

is available by managing the gating bar in the four different channels.

1. Click the histogram of each channel, then the cell size graph will

be shown. X-axis indicates cell size and Y-axis indicates number

of cells.

2. Press ‘Apply’ button.

3. Blue lines are indicating the gating condition. It can be adjusted

by moving the bar.

⑦ Raw data table

The number of events for each frame will be displayed by channels.

Frame by frame revision of raw data image and removal of invalid

frames can be done on the table. For deleting an invalid frame, click

on the right mouse button and select ‘Delete’ menu. Then, the “Result

area” will show the re-calculated data, reecting the deleted frame(s).

Operation

Data menu

⑦ Raw data table

How to nd the invalid frames is described below:

1) Sample aggregation: when there is aggregation in specic area

of sample, that frame should be deleted.

Br image

2) Dust: when there is dust inside the slide, that frame should be

deleted.

Br image

FL image

3) Bubble: After loading the sample, if there is bubble in the slide,

that frame should be deleted.

Br image

FL image

Operation

Data menu

⑦ Raw data table

4) Cell debris: when there is cell debris, that frame should be deleted.

Br image

5) Overlapped circle: when there are several circles in one cell, that

frame should be deleted.

Br image

FL image

Operation

Data menu

⑧ Data list

⑨ Export data

To review the data that is saved in ADAMII™, select the desired sample from

the data list. Scroll up and down to check the data of sample.

Data list

ADAMII™ allows you to export the data table(.csv), images(.jpg),

and the nal report(.pdf). Click ‘All’ button to export the data in 3

types of formats at once.

• csv le: data table containing comprehensive information in a

spreadsheet

• jpg le: raw images of each channel collected for each eld of view

• pdf le: a nal report containing an aggregate result and

calculations such as the frames, volume, event count and

viability of the sample

[csv le]

[jpg le]

[pdf le]

Operation

Data menu

⑩ Edit data list

User can edit the data list. Please refer to below explanation of each

function.

• Rename: To rename a le, select the data le and click ‘Rename’

button to change name of the selected data.

• Select all: Click ‘Select all’ button to select all of the listed data.

You can also select all of the sample data at once by

pressing ‘Shift’ button.

• Delete: To delete a le, select the data le and click ‘Delete’ button.

Operation

Setting menu

Setting menu contains information of ADAMII™ as well as several

user preferences and other Settings. It allows users to edit settings

about the basic operation, software and rmware information.

1. Date & Time

2. Counting settings

3. Calibration

4. Sample volume

5. Entry required

6. Update

7. Device info

8. Disk info

Operation

Setting menu

① Date & Time

② Counting settings

The date and time are already preset when you receive the instru-

ment. You can reset the date and time by yourself.

You can use the adjustment options below if you think that the

counting results are not adequate.

• Sensitivity: refers to the contrast of the objects from the background

- Adjusting the sensitivity higher makes instrument

more sensitive to objects

- While adjusting the sensitivity lower makes the

instrument less sensitive and is useful if there is a lot

of background

• Circularity: used to determine which objects to include in the

measurement based on their roundness

- Increasing the value from 80% requires objects to

be more round for inclusion in the measurement

- Decreasing the value from 80% allows objects to

be less round

- Adjusting this may be useful if the cell type is not

particularly circular or perhaps oddly shaped

Operation

Setting menu

③ Calibration

④ Sample volume

Please refer to page 12~19 for calibration.

To open the calibration result list, click ‘Record’ button. You can

check the data of calibration briey including date, time, lot number,

user ID and result of each channel.

Select the sample type. Enter the total volume (sample volume +

RBC lysis buffer) and sample volume. Then click ‘Set’ button to apply.

⑤ Entry required

Set entry option of the sample ID and Patient ID.

Operation

Setting menu

⑥ Update

Check the software and rmware versions to update.

[Software update]

1. Prepare the updated software in a USB drive and plug into the laptop.

2. Press ‘SW update’ button.

3. Find the updated software in the USB drive and double click the

le to execute.

4. When you see the window below, the update completed successfully.

Press OK button to proceed.

5. Program will be restarted automatically. Check that the version of

software was modied properly.

Operation

Setting menu

⑥ Update

[Firmware update]

1. Save the rmware on USB disk and connect to the laptop.

2. Press ‘FW update’ button.

3. Move to the folder where ‘Firmware update le’ is located and

click the le.

4. Press OK button when the automatic update is completed.

5. Program will be restarted automatically. Check the updated

rmware version.

Operation

Setting menu

⑦ Device info

⑧ Disk info

Device info indicates serial number of Instrument and connecting

status between cable and the instrument. Please check the connec-

tion status if ‘Disconnected’ is displayed.

Disk info indicates available hard disk space. Please back up or

delete your data from the hard drive when the free space is less

than 100MB.

Cleaning and Maintenance

Clean the surface of ADAMII™ instrument with a damp cloth. If liquid spills on ADAMII™, turn off the

power immediately and wipe dry.

ADAMII™ does not need regular maintenance. To troubleshoot problems with ADAMII™, contact

technical support.

IMPORTANT! Never disassemble or service ADAMII™ by yourself.

Unauthorized repairs may damage ADAMII™ or alter its functionality, which will void your

warranty. Contact ivdst@nanoentek.com or your local distributor to arrange for service.

IMPORTANT! Always wipe surfaces with ethanol-soaked paper towels.

Do not directly spray ethanol anywhere on ADAMII™.

IMPORTANT! Avoid exposing ADAMII™ to UV light.

UV light may degrade components, including plastic. Damage from UV exposure is not covered under the manufacturer’s warranty.

Troubleshooting

Installation

ADAMII™

does not power on

Operator program

does not start

Camera preview

Camera preview fail

Operation

Warning message

when there are more than

1,000 cells in 1 frame

Total CD45 count is higher than

bright count (WBC count)

Total CD45 count is lower than

bright count (WBC count)

Frame becomes out of focus

towards the end

• Check on/off switch on back side of main instrument.

• Check power source or contact your distributor

• Check on/off switch on back side of main instrument.

• Check connection between instrument and PC

• Reboot the instrument or PC

• Request technical support

• Dilute the cell concentration less than 1,000 cells/μL

• Recommend 500 ~ 900 cells in 1 frame

• Delete image that has bubble, uorescence debris or dust,

and then check the result

• Check the focus of image

• If the focus of image was not set properly, load and

read the sample again

• Change the slide and read again

• If you are still having problem, request technical support

Frame is in focus and then out of

focus. The following frame is in fo-

cus again. It happens repeatedly.

Focusing is not adjusted well

with autofocus function

Background of image is

suddenly brighten

• Request technical support

• Set the focus with 2ch manual focus function

• First, check the sample type

• Second, if you used the bottom of ADAMII™ CD34 Reagent

that almost used, please use the new reagent

• If none of the above 2 steps solve the problem, please

request technical support

Troubleshooting

Operation

• First, vortex enough for 5 seconds and load sample immediately to read

the slide again

• Second, check the focus. When the image is out of focus, the uorescence

Calibration fail

Sensor of slide

holder cap is faulty

Inaccurate result

of bead is unclear. If the focus is not set continuously in reading step,

please request technical support

• If the focus is still not set correctly even you did both the above rst and

second steps, change the bead

• If none of the above 3 steps solve the problem, please request technical support

• ADAMII™ does not inser t and count the slide when the slide holder cap opened.

• But if sensor of slide holder cap is faulty and you start counting without

closing the cap, then the result will not be accurate. When you eject

the slide with the door open, the cap can be broken inside the instrument.

Poor image quality

• While viewing cells in preview state, re-optimize the focus.

Error code

Error code Reason Message Position

E0001

E0002

E0010

E0020

E0030

Camera

connection fail

Serial

connection fail

Serial

disconnected

Camera

disconnected.

Open the slide

holder

Driver

Stage

working

Camera

Slide

inserting

E0031

Open the slide

door

Slide

ejecting

Error code

Error code Reason Message Position

E0040

E0050

E0060

Data storage

is full

Sample density

is too high

Update fail

Data save

Measuring

SW update

Warranty

NanoEnTek provides 1-year warranty service for defects of material and workmanship.

If any defects occur in ADAMII™, NanoEnTek provides repair services for the defective parts at its

discretion.

The following defects, however, are specically excluded:

1. Defects caused by improper operation.

2. Repair or modication done by anyone other than NanoEnTek or an authorized agent.

3. Damage caused by substituting alternative parts.

4. Use of ttings or spare parts supplied by anyone other than NanoEnTek.

5. Damage caused by accident or misuse.

6. Damage caused by disaster.

7. Corrosion caused by improper solvent or sample.

For your protection, ADAMII™ units being returned must be insured against possible damage or

loss. NanoEnTek cannot be responsible for damage incurred during shipment of a defective instru-

ment. It is recommend that you save the original packing material in which the instrument was

shipped. This warranty is limited to the replacement of defective products.

For any inquiry or request for repair service,

please contact ivdst@nanoentek.com or your local distributor.

Safety precautions

Review and follow the safety instructions below:

• If water or other material enters the instrument, adaptor, or power inlet, disconnect the power

cord and contact a service person. For operating environment, refer to Product Specications.

• Do not touch the main plug or power cord with wet hands.

• Always ensure that the power supply input voltage matches the voltage available at your location.

• This instrument is air-cooled and its surfaces may become hot during operation.

During installing, leave a space of more than 10 cm (4 inches) around the instrument and do not

place any objects between the instrument and the walls.

• Do not install the instrument on a slant or a place prone to vibrations, which induces the risk of

instrument malfunction or damage of the instrument.

• Never insert any objects into the air vents of the instrument as this could result in electrical shock,

personal injury, and equipment damage.

• Plug the power cord rmly into the wall outlet and AC adapter.

• To avoid potential shock hazard, make sure that the power cord is properly grounded.

• Be sure to position the equipment such that it is easy to disconnect.

• Turn off the instrument before unplugging the power cord and/or moving the instrument.

• If the instrument is dropped or broken, disconnect the power cord and contact a service person.

Disassembly of case will void warranty.

• Use only authorized accessories (adaptor, power cord, and USB drive).

Warning:

Class A equipment is intended for use in an industrial environment.

In the documentation for the user, a statement shall be included drawing attention to the fact that

there may be potential difficulties in ensuring electromagnetic compatibility in other environments, due

to conducted as well as radiated disturbances.

Consignes de securite

Examinez et suivez les consignes de securite ci-dessous :

· N’installez pas l’instrument dans un endroit humide comme une serre ou un incubateur pour eviter un risque de choc electrique. Si de l’eau ou tout autre materiau penetre dans l’instrument, l’adaptateur, ou l’entrees d’alimentation, debranchez le cordon d’alimentation et contactez un tech nicien de service. Pour l’environnement d’exploitation, reportez-vous aux specications du produit.

· Ne touchez pas la che ou le cordon d’alimentation principale avec les mains mouillees.

· Assurez-vous toujours que la tension d’entree d’alimentation correspond a la tension disponible dans votre endroit.

· Cet instrument est refroidi a l’air de sorte que ses surfaces peuvent devenir chaudes pendant le fonctionnement.

· Lors de l’installation de l’instrument, laisser un espace de plus de 10 cm (4 pouces) autour de cet instrument et ne placez aucun objet entre l’appareil et le mur.

· N’installez pas l’instrument sur une pente ou un endroit soumis a des vibrations, ce qui induit le risque de dysfonctionnement ou d’endommagement de l’instrument.

· N’inserez jamais aucun objet dans les orices d’aeration de l’instrument, car cela pourrait entrainer un choc electrique, des blessures chez les utilisateurs et des dommages d’equipement.

· Branchez le cordon d’alimentation fermement dans la prise murale et l’adaptateur secteur aussi.

· Pour eviter un risque potentiel de commotion electrique, assurez-vous que le cordon d’alimentation est correctement mis a la terre.

· Assurez-vous de positionner l’instrument de telle sorte qu’il soit facile de debrancher l’instrument.

· Eteignez l’instrument avant de debrancher le cordon d’alimentation et / ou de deplacer l’instrument.

· Si l’instrument est casse ou qu’il soit tombe, debranchez le cordon d’alimentation et contactez un technicien de service. Ne demontez pas l’instrument et la garantie sera annulee en cas de demontage.

· Utilisez uniquement les accessoires autorises (l’adaptateur, le cordon d’alimentation, et le lecteur USB).

Prudence

Le produit de classe A est concu pour l’utilisation dans un environnement industriel. Dans la documentation de l’utilisateur, la declaration doit etre incluse pour attirer l’attention sur le fait qu’il peut y avoir des difficultes potentielles pour assurer la compatibilite electromagnetique dans d’autres environnements, en raison des perturbations rayonnees et.

Safety precautions

Review and follow the safety instructions below:

Symbol Meaning

Caution or Warning

Protective earth (Ground)

This instrument and consumables conforms to the EC Declaration of

Conformity.

This equipment has been tested and found to comply with the limits for a

Class A digital device, pursuant to Part 15 of the FCC Rules.

These limits are designed to provide reasonable protection against

harmful interference when the equipment is operated in a commercial

environment. This equipment generates, uses, and can radiate radio

FCC Compliance

frequency energy and, if not installed and used in accordance with the

instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful

interference in which case the user will be required to correct the

interference at his own expense.

Disposal of your old appliance 1. When this crossed-out wheeled bin

symbol is attached to a product it means the product is covered by the

European Directive 2012/19/EU. 2. All electrical and electronic products

should be disposed of separately from the municipal waste stream via

designated collection facilities appointed by the government or the local

authorities. 3. The correct disposal of your old appliance will help prevent

potential negative consequences for the environment and human health.

4. For more detailed information about disposal of your old appliance,

please contact your city office, waste disposal service or visit our

web-site, www.nanoentek.com.

This product conforms to UL 61010-1, CAN/CSA C22.2 No.61010-1

“Safety Requirements for Electrical Equipment for Measurement, Con-

trol, and Laboratory Use, Part 1: General Requirements. “Instruments

bearing the TUV symbol are certied by TUV Product Services to be in

conformance with the applicable safety standard for the US and Canada.

Product specications

ADAMIITM Instrument

• Light source:

Bright, Yellow (PE), Red (PerCP) and Green (FITC)

• Fluorescence:

PerCP: BLP01-635R (650 long pass)

PE: Excitation 525/50, Emission 572/28-25

FITC: Excitation 466/40, Emission 550/50

• Camera: High-sensitivity monochrome CCD

• Stage: Automated X-Y-Z stage

• Objective lens: Olympus lens (10X) / 35∮ tube lens

• Exported formats: Image: JPEG, BMP / Raw data: CSV

• Operating power: 100 – 240 V, 1.5 A, 50/60 Hz

• Electronic input: 12VDC, 5.0A

• Operating environment: 5 - 40 °C, 20 – 95 %

• Weight: 19.3 kg

• Dimensions: 300 x 420 x 370 mm (L x W x H)

Operating laptop

• CPU: 6th Generation Intel® Core™ i3-6100U Processor 

• OS: Windows 8.1 (64 bit)

• RAM: 4 GB DDR3L 1600MHz

• HDD: 500GB 7200rpm Hard Drive

• 14” Full HD (1920 X 1080) Anti Glare(16:9) WLED

Ordering information

Cat. No. Description Contents

ADAM2

ADAM2-PC

ADAMII

Fluorescence Cell Counter

ADAMII

, Bench-top

Laptop PC

· Main instrument

· Power cord

· USB connection cable

· CPU : 6th Generation Intel® Core™ i3-6100U Processor 

· OS : Windows 8.1 (64 bit)

· RAM : 4 GB DDR3L 1600MHz

· HDD : 500GB 7200rpm Hard Drive

· 14” Full HD (1920 X 1080) Anti Glare(16:9) WLED

Technical support

Visit the our Website at www.nanoentek.com for :

· Technical resources, including manuals, FAQs, etc.

· Technical support contact information

· Additional product information and special offers

For more information or technical assistance, please call or email.

ivdst@nanoentek.com

Website

www.nanoentek.com

Manufactured by:

NanoEnTek Inc.

851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea

Tel : +82-2-6220-7942,

Fax : +82-2-6220-7999

MT Promedt Consulting GmbH

Altenhofstrasse 80, 66386 St. Ingbert, Germany

US Branch

NanoEnTek USA Inc.

5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA

Tel.: +1-925-225-0108, +1-888-988-0108(Toll free),

Fax.: +1-925-225-0109

Memo

ADAMII™

NESMU-ACD-001E (V.0.0)

ivdst@nanoentek.com

Website

www.nanoentek.com

Manufactured by:

NanoEnTek Inc.

851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea

Tel : +82-2-6220-7942,

Fax : +82-2-6220-7999

MT Promedt Consulting GmbH

Altenhofstrasse 80, 66386 St. Ingbert, Germany

US Branch

NanoEnTek USA Inc.

5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA

Tel.: +1-925-225-0108, +1-888-988-0108(Toll free),

Fax.: +1-925-225-0109

Nanoentek Adamii User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual