Nanoentek Adamii User Manual

Bench-top Fluorescence Cell Counter

User manual

All the materials in this user manual are protected by Korean and international copyright laws.

They cannot be reproduced, translated, published or distributed without the permission of the

Every effort has been made to ensure that all the information contained in the ADAMII™ User

Manual is correct at the time of printing. However, NanoEntek Inc. reserves the right to make any

changes necessary without notice as part of ongoing product development.

ADAMII™ User Manual

Website: www.nanoentek.com

E-mail: ivdst@nanoentek.com

Documentation: NESMU-ACD-001E(V.0.0)

Revision history: V.0.0 Date: March 2017

Table of contents

Introduction

Product components

Product description

Installation

Home menu

Calibration

Sample loading

Operation

- Insert assay slide

- Measure menu

· Initial mode

· Focusing mode

- Data menu

- Setting menu

Cleaning & Maintenance

Troubleshooting

Error code

Warranty

Safety precautions

Safety symbols

Product specications

Ordering information

Technical support

6-7

8-9

10-11

12-19

21-49

21-23

24-33

24-26

27-33

34-43

44-49

51-52

53-54

56-57

Introduction

ADAMIITM is a bench top uorescent cell counting analyzer that can be used in either a hospital and

research laboratory.

It has 4-channel (Bright, Yellow (PE), Red (PerCP) and Green (FITC)) light sources, so three color

uorescence samples can be analyzed simultaneously. The automatic X-Y-Z stage captures a maxi-

mum of 136 images and which increases the accuracy of analysis.

The obtained images are processed by image analysis software integrated in the system. All proce-

dures are automatic once the stained sample has been placed in the disposable assay slide.

[Features]

• A benchtop uorescence cell counting analyzer

• Can be used in hospital and research laboratory

• Visualize: Quantitative results with visual conrmation

• Quantify uorescence-labelled cells without sheath tank maintenance

Product components

ADAMII™ is shipped with the following components. Once you receive your instrument, please

check that all components listed below were shipped.

If any component is missing or damaged, contact your local distributor or e-mail ivdst@nanoentek.com

ADAMII™

1 EA

User manual

1 EA

Power cord with 4 adaptor cords

(for U.S./Canada/ Taiwan/Japan,

Europe or UK)

4 pcs/1 SET

Focus guide

1 EA

USB cable

2 EA

Operating laptop

(Please note: Laptop will be packaged separately)

1 set

Product description

Front view

Side view

Insert/Eject

button

Run button

State LED

Slide Holder

Door

Insert/Eject Button

Run Button

State LED

Insert and eject the slide from the ADAMII™.

Functions as load and unload.

Perform all procedures of automatic counting.

Light up when software runs in the laptop and

connection of camera and serial perform cor-

rectly.

Focus Knob

Slide Holder

Door

Focus Knob

Location to insert or remove the slide.

Open to check the slide holder.

Adjust focus to obtain better cell images.

Product description

Rear view

Power

supply port

Power supply port

Power switch

Serial connection port

Camera connection port

Power switch Serial

Serial Camera

connection port

Power supply

Power on/off

Control the ADAMII™ operating

Control the camera of the ADAMII™

Camera connection port

Installation

Unpacking the instrument

Install the instrument

1. Open the box and remove the material foam in the box.

2. Carefully lift the instrument out of the box.

3. Place the instrument on a at, level surface.

1. Plug the power cord into the electrical outlet and connect two USB cables..

Power supply port USB port

Serial Camera

2. Connect the laptop and the ADAMII™ using two USB cables.

Installation

Install SW program

1. Turn on the laptop and ADAMII™ by pressing the Power button. At

this time, the slide holder will be ejected automatically.

2. Double-click ADAMII™ icon on Desktop to start the software.

☞

Note: The ADAMII™ will be ready to use after initialization is

completed when powered on.

☞

Note: When the software runs and is connected with ADAMII™

normally, the state LED of instrument will be lighted up.

CAUTION:

• You must turn on the ADAMII™ before running the software program.

Home menu

User management

At the ‘Home’ screen, enter the user ID and password to log in.

Default ID and password are both ‘master’.

Click ‘User management’ when you need to manage accounts (e.g. ID

addition, password change). To create a new ID, ll out the blank (user

name, user ID, password, conrm PW) and click ‘Add’.

☞

Note: Only the master user can access the ‘User management’ .

Home menu

Audit trail

Click ‘‘Audit trail” to search and trace detailed information of each user.

1. Enter the User ID and set the date that you want to search.

2. Click ‘Search’ button.

3. Information of the selected user will appear.

☞

Note: Only the master user can access the ‘‘Audit trail”.

Calibration

Calibration procedure

1. For calibration, go to ‘Setting’ menu and click ‘Calibrate’.

2. Then, calibration window will appear.

Calibration

Calibration procedure

3. Shake bottle vigorously or vortex briey for 5 seconds before use.

5 sec.

[Shake bottle vigorously]

4. Once again, vortex for 4 seconds and pipette for 25 μL immediately.

4 sec.

[Vortexing]

[Pipetting calibration bead 25 μL]

[Vortexing]

5. Load 25 μL of calibration beads on the ADAMII™ assay slide.

[Load 25 μL calibration bead]

Calibration

Calibration procedure

6. Attach dry-proof closure tape to prevent drying.

[Attach dry-proof closure tape]

Warning:

• Calibration bead loading error

- Ensure that 25μL of calibration bead is fully loaded on the ADAMII™

assay slide.

- The instrument will not detect in low or high loading volumes.

CAUTION:

• Avoid bubbles as theymay negativelyaffect results.

Low volume Correct volume

• Boundary between black and white on the tape should be lined up

with the edge of the slide.

Dry-proof closure tape

Calibration

Calibration procedure

7. Leave for 20 seconds (need time for beads to settle).

20 sec.

8. Insert the slide with calibration beads into the slide port of the

instrument and press ‘Insert sample’ button.

9. Enter lot number of calibration beads.

10. Select Bright channel and adjust the focus using the focus knob.

Calibration

Calibration procedure

☞

Note: Please check ‘ADAMII™ Focus guide’ before setting the focus.

[Focusing example - representative images]

Bright- Good Focus

Good Focus VS. Bad Focus

FL- Good Focus

• Bright - Good Focus: Clear circumference of the beads, Dark and

clear contrast to the background

• Bright - Bad Focus: Blurry and unclear bead circumference

• FL - Good Focus: Bright uorescence beads with clear contrast to the

background

• FL - Bad Focus: Weak uorescence beads with unclear contrast to the

background

Bright- Bad Focus

FL- Bad Focus

Calibration

Calibration procedure

11. Press the ‘Save BR Focus’ after adjusting focus.

12. Select PerCP channel and adjust the focus using the focus knob.

13. Press the ‘Save FL Focus’ after adjusting focus. The focus of other

uorescence channels will be set automatically.

☞

Note: Save the focus in ‘Bright’ and ‘PerCP’ channel.

The other channels will be automatically focused.

14. Press the ‘Run’ button to start the auto calibration. Calibration will

take about 3 minutes to count the 24 frames.

3 min.

Calibration

Calibration procedure

15. When the calibration is completed, the results will be displayed.

☞

Note: If the results are not within the range on the tube,

please ask your manufacturer or distributor.

16. When you need previous results of calibration, click ‘Record’ button.

Calibration

Calibration procedure

17. Calibration result list records will appear.

18. Click ‘Save’ button to save the calibration records. The result will be

saved in CSV format.

19. Press “Close” button to nish calibration.

Sample loading

Sample preparation

Load sample

Prepare sample and check the sample type.

To run a sample, click ‘Measure’ menu.

Load 25 μL sample into the ADAMII™ assay slide and attach a dry-

proof closure tape.

[Load 25 μL sample] [Attach dry-proof closure tape]

Note: Please refer to page 14 to check cautions for loading correct

☞

volume and attaching the dry-proof closure tape.

☞

Note: Note: Before loading the antibody stained specimen or bead to

the slide, vortex enough to mix them up.

+ 44 hidden pages

Nanoentek Adamii User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual