Multichannel Systems PPS2 User Manual
PPS2 Peristaltic Perfusion System
Manual
Information in this document is subject to change without notice.
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or for damages resulting from the use of information contained in this document
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damage caused or alleged to have been caused directly or indirectly by this document.
© 2013 Multi Channel Systems MCS GmbH. All rights reserved.
Printed: 13.05.2013
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25- 0
Fax +49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
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Table of Contents
1 Important Information and Instructions 2
1.1 Operator's Obligations 2
1.2 Guarantee and Liability 2
1.3 Important Safety Advice 3
2 Installation and Operation 4
2.1 Welcome to the PPS2 Peristaltic Perfusion System 4
2.2 Setting Up and Connecting the PPS2 Pump 5
2.2.1 Use of Droplet Isolators 6
2.3 Control Options 9
2.3.1 Operation of the Perfusion Peristaltic Pump with PPS2
Software 9
2.3.2 Operation of the Perfusion Peristaltic Pump via PPS2
Touch Screen 14
2.4 Cleaning and Maintenance 16
PPS2 Manual
3 Application Example: MEA2100-System 17
3.1 Setup 17
3.2 Perfusion and Noise 18
4 Appendix 19
4.1 Technical Specifications 19
4.2 Ordering Information 20
4.2.1 Scope of Delivery 20
4.2.2 Replacement 20
4.2.3 Data Sheet 21
4.3 Contact Information 22
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Peristaltic Pump Manual
1 Important Information and Instructions
1.1 Operator's Obligations
The operator is obliged to allow only persons to work on the device, who
are familiar with the safety at work and accident prevention regulations and have been
instructed how to use the device;
are professionally qualified or have specialist knowledge and training and have received
instruction in the use of the device;
have read and understood the chapter on safety and the warning instructions in this manual
and confirmed this with their signature.
It must be monitored at regular intervals that the operating personnel are working safely.
Personnel still undergoing training may only work on the device under the supervision
of an experienced person.
1.2 Guarantee and Liability
The General conditions of sale and delivery of Multi Channel System MCS GmbH always apply.
The operator will receive these no later than on conclusion of the contract.
Guarantee and liability claims in the event of injury or material damage are excluded
when they are the result of one of the following.
Improper use of the device.
Improper installation, commissioning, operation or maintenance of the device.
Operating the device when the safety and protective devices are defective and / or inoperable.
Non-observance of the instructions in the manual with regard to transport, storage,
installation, commissioning, operation or maintenance of the device.
Unauthorized structural alterations to the device.
Unauthorized modifications to the system settings.
Inadequate monitoring of device components subject to wear.
Improperly executed and unauthorized repairs.
Unauthorized opening of the device or its components.
Catastrophic events due to the effect of foreign bodies or Acts of God.
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PPS2 Manual
1.3 Important Safety Advice
Warning: Make sure to read the following advice prior to install or to use the device
and the software. If you do not fulfill all requirements stated below, this may lead
to malfunctions or breakage of connected hardware, or even fatal injuries.
Warning: Obey always the rules of local regulations and laws. Only qualified personnel
should be allowed to perform laboratory work. Work according to good laboratory practice
to obtain best results and to minimize risks.
Warning: Be very careful with the rotating parts of the pump! Do not clamp something
in between neither long hair nor a cravat, for example.
The product has been built to the state of the art and in accordance with recognized safety
engineering rules. The device may only
be used for its intended purpose;
be used when in a perfect condition.
Improper use could lead to serious, even fatal injuries to the user or third parties and damage
to the device itself or other material damage.
Warning: The device and the software are not intended for medical uses and must not
be used on humans.
Malfunctions which could impair safety should be rectified immediately.
Requirements for the installation and operation
Do not use the PPS2 peristaltic pump for perfusion with flammable or aggressive
(corrosive) liquids.
Do not store flammable materials nearby during operation.
Check in regular intervals that the PPS2 pump does not overheat.
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Peristaltic Pump Manual
2 Installation and Operation
2.1 Welcome to the PPS2 Peristaltic Perfusion System
The peristaltic pump PPS2 with software control is developed for the perfusion of
biological samples. The pumps are driven by stepper motors providing a very long lifetime
without maintenance. The durable brushless motors are extremely reliable, show very
constant rotation speed, are vibration free and have low electromagnetic emission. These
characteristics make the PPS2-System an ideal choice for electrophysiological experiments.
The device consists of two independent peristaltic pumps, one perfusion (inlet) pump
for delivering perfusion solution to the sample, and one waste (outlet) pump to remove
dispensable solution. The droplet isolator chambers avoid pulsation artifacts and allow
optical fluid control.
If you need more than two channels you can connect several PPS2-Systems in serial.
Each pump can still be controlled separately.
The flow rate of the pumps vary between 0 to 30 ml/minute. Other flow rates are available
on demand, please contact MCS.
The PPS2 is a highly flexible system which can be controlled in different ways:
The pump velocity and the flow rate can be controlled from a connected computer
via USB connection and the included software PPS2.
In future versions it will be possible to control the PPS2 with the LTP-Director software
from Multi Channel Systems MCS GmbH. Please see chapter “Software Control”.
Additional analog and digital inputs allow to operate the PPS2 pump via external
inputs, for example from a stimulus generator STG.
It is also possible to operate the pump without software control, via touch screen
on top of the device.
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2.2 Setting Up and Connecting the PPS2 Pump
Warning: Do not start the perfusion until you have double-checked that the perfusion lines
are set up properly and that the inflow and outflow rate are matching. Spilled liquid may
irreversibly damage electronic instruments.
The PPS2 consists of two independent peristaltic pumps, which both are rotating
counterclockwise. The left one is intended for perfusion inlet, the right one for perfusion
outlet. It is of course also possible to use both pumps as independent perfusion inlet pumps,
and to use for example a vacuum pump for suction. This manual will focus on the intended
function as inlet and outlet pumps. Each pump cycle contains a droplet isolator. The outlet
pump is additionally equipped with a bubble detector.
Connecting the Tube Set
The following tubes are used:
Identical Pharmed ® BPT (Saint–Gobain) tubes with an inner diameter of 1.65 mm
and an outer diameter of 3.35 mm around the pump revolver and for the connection
between outlet droplet isolator chamber and outlet pump, where the sensor of the
bubble detector is located.
PPS2 Manual
A PVC tube with larger inner diameter is used for the tube to the waste bottle.
Silicon tubing is used for all other connections.
The specified tubing must be used for the pump revolvers to ensure that the volumes set
in the software are correct. Other tubing can be used for all other connections as well.
Sets of ten tubes for replacement are available from Multi Channel Systems (For example:
PPRT1.65-10 tubes: Pharmed ® BPT, ID: 1.65 mm, OD: 3.35 mm). Additionally you can order
a replacement set for all fluidic components PPS2-Set-F. Please see section “Ordering
Information” in the Appendix.
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Peristaltic Pump Manual
For connecting or changing the tubes, please open the screw and remove the bracket of the
pump revolver. Insert the provided tube and fix it with the luer tube connectors in the denoted
way. To remount the bracket, take care to insert the bar of the bracket into the corresponding
socket on the pump housing. Fix the screw firmly.
If you do not need the droplet isolator, please use the circlet and connect the heads
of the tube with a luer adapter.
2.2.1 Use of Droplet Isolators
The droplet isolators can be used to disrupt the direct liquid column from the pump to the
sample. This can help to remove low frequency noise originating from the pulsation of the
pump head. On the other hand, the droplet isolators make the flow speed inaccurate, and
cause a continued flow even after the pump is stopped. If the flow rate is high (approximate
> 6 – 8 ml/min) the droplet isolators will fill up over time and might even overflow.
The suggested procedure is to start working without the droplet isolators, and use them only
in case unacceptable fluctuations caused by the pump occur in the recordings which can not
be removed otherwise, for example by using a 10 Hz high pass filter.
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