USB-MEA256-System Manual
Information in this document is subject to change without notice.
No part of this document may be reproduced or transmitted without the express written
permission of Multi Channel Systems MCS GmbH.
While every precaution has been taken in the preparation of this document, the publisher
and the author assume no responsibility for errors or omissions, or for damages resulting from
the use of information contained in this document or from the use of programs and source code
that may accompany it. In no event shall the publisher and the author be liable for any loss of
profit or any other commercial damage caused or alleged to have been caused directly or
indirectly by this document.
4 Multi Channel Systems MCS GmbH. All rights reserved.
© 201
Printed: 17. 04. 2014
Multi Channel S
ystems MCS GmbH
Aspenhaustraße 21
72770 Reutlingen
Germany
Fon +49-71 21-90 92 5 - 0
Fax +49-71 21-90 92 5 -11
info@multichannelsystems.com
www.multichannelsystems.com
Microsoft and Windows are registered trademarks of Microsoft Corporation. Products that
are referred to in this document may be either trademarks and/or registered trademarks of
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no claim to these trademark.
Table of Contents
1 Introduction 1
1.1 About this Manual 1
1.2 Welcome to the USB-MEA256-System 2
2 Important Safety Advice 3
2.1 Operator's Obligations 3
2.2 Guarantee and Liability 3
2.3 Important Safety Advice 4
3 Software Installation 5
3.1 Software Installation 5
3.1.1 System Requirements 5
3.1.2 Recommended BIOS settings 6
3.1.3 Driver Installation 6
3.1.4 First Use of MC_Rack 7
4 USB-MEA256-System 9
4.1 The USB-MEA256 Data Acquisition 9
4.2 The USB-MEA256 Filter Amplification 9
4.3 256MEA 10
4.4 256 Thin MEA 11
4.5 256-9well MEA 11
4.6 The USB-MEA256-System 12
4.7 Connecting the USB-MEA256-System 12
4.8 Setting Up the Amplifier 13
4.9 The USB-MEA256 Device 19
4.9.1 Rear Panel 19
5 Troubleshooting 23
5.1 Troubleshooting 23
5.2 No Computer Connection / No Recording Possible 23
5.3 Triggering / Digital Input does not Work 24
5.4 Noise on Single Electrodes 24
5.5 MEA is defective 25
5.6 Overall Noise / Unsteady Baseline 25
5.7 Missing Spikes or Strange Signal Behavior 26
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USB-MEA256-System Manual
6
Appendix 27
6.1 Technical Support 27
6.2 Technical Specifications 28
6.3 Pin Layout 30
6.4 Test Model Probe 32
6.5 Data Sheet 256MEA 33
6.6 Data Sheet 256ThinMEA 34
6.7 Data Sheet 256MEA-STIM-C-Con 36
6.8 Data Sheet 256-9wellMEA 35
6.9 Data Sheet 256MEA-9well-STIM-ADPT 37
6.10 256MEA-Signal Generator 38
6.11 Digital IN / OUT Extension 39
6.12 Scope of Delivery 40
6.13 Contact Information 40
7 Index 41
iv
1 Introduction
1.1 About this Manual
It is assumed that you already have a basic understanding of technical and software terms.
No special skills are required to read this manual.
If you are using the device for the first time, please read the Important Safety Advice before
installing the hardware and software, where you will find important information about the
installation and first steps.
The device and the software are part of an ongoing developmental process. Please understand
that the provided documentation is not always up to date. The latest information can be found
in the Help. Check also the MCS web site (www.multichannelsystems.com) for downloading
up-to-date manuals and help files.
Welcome to USB-MEA256-System
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USB-MEA256-System Manual
1.2 Welcome to the USB-MEA256-System
The USB-MEA256-System is a compact and portable stand-alone solution for MEA recordings
with integrated amplification, data acquisition, and analog / digital conversion.
It is a highly flexible system: The system acquires data from up to 252 electrode channels,
four additional analog channels, and 16 digital IN / OUT channels. The digitized electrode data
is transmitted to the connected computer via universal serial bus (High Speed USB 2.0). Thus,
it is possible to use any computer as a data acquisition computer, also a laptop.
Data is recorded, graphed, analyzed, and reviewed with the powerful and easy-to-use MC_Rack
program from Multi Channel Systems MCS GmbH. You can export the data in standard formats
to other programs with the software MC_DataTool. Instead of MC_Rack any other common data
processing program or custom software can be used.
The integrated filter amplifier supports 252 electrode channels. The amplification factor
of 1100 is fixed. The bandwidth of 1 Hz to 5 kHz is suitable for a broad range of applications,
such as spike and field potential recording from neurons or recording of cardiac signals.
Electrode raw data are acquired from the 256MEA and digitized by the analog / digital
converter board that is integrated into the main unit. Recorded signals are converted in real
time into digital data streams at sampling rates of up to 40 kHz per channel. You will not miss
even fast biological signals. Data is transferred to the computer via High Speed USB 2.0 port.
The USB-MEA256-System features a heating element with a PT100 temperature sensor.
If you connect a temperature controller TCX to the heating element via D-Sub 9 connector,
the heating element guarantees constant temperature conditions for the biological sample,
placed on the 256MEA (Microelectrode Array with 252 recording electrodes and four reference
electrodes) or on the 256ThinMEA or the 256-9wellMEA respectively.
To control the biological sample on the 256MEA inside the amplifier optically, you can use either
an upright microscope or an inverted microscope.
A 16-bit digital input / output (TTL) is available. You can use the digital TTL inputs, for example,
for synchronizing stimulation and recording, or for synchronizing the USB-MEA256-System with
other systems. The digital TTL outputs can be used for triggering other devices, for example,
an imaging setup.
The 16 bit Digital IN / OUT channel can be contacted with a 68-pin MCS standard connector.
The bits 0 from the D IN and the D OUT, respectively, are also accessible independently by
the D0 IN and D0 OUT Lemo connectors.
The additional analog inputs 1 to 4 are intended for recording additional information from
external devices, for example, for recording patch clamp in parallel to the MEA recording,
for monitoring the temperature, or for recording voice.
To the Audio Out (3.5 mm phone jack) you can connect an audio system to make the electrical
activity audible. This audio output is real time. Headphones or a speaker can be connected directly
to the AUDIO OUT. Only one channel at a time can be converted into sound.
2
2 Important Safety Advice
2.1 Operator's Obligations
The operator is obliged to allow only persons to work on the device, who
are familiar with the safety at work and accident prevention regulations and have been
instructed how to use the device;
are professionally qualified or have specialist knowledge and training and have received
instruction in the use of the device;
have read and understood the chapter on safety and the warning instructions in this manual
and confirmed this with their signature.
It must be monitored at regular intervals that the operating personnel are working safely.
Personnel still undergoing training may only work on the device under the supervision
of an experienced person.
2.2 Guarantee and Liability
The General conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply.
The operator will receive these no later than on conclusion of the contract.
Multi Channel Systems MCS GmbH makes no guarantee as to the accuracy of any and all tests
and data generated by the use of the device or the software. It is up to the user to use good
laboratory practice to establish the validity of his / her findings.
Guarantee and liability claims in the event of injury or material damage are excluded when
they are the result of one of the following.
Improper use of the device.
Improper installation, commissioning, operation or maintenance of the device.
Operating the device when the safety and protective devices are defective and/or inoperable.
Non-observance of the instructions in the manual with regard to transport, storage, installation,
commissioning, operation or maintenance of the device.
Unauthorized structural alterations to the device.
Unauthorized modifications to the system settings.
Inadequate monitoring of device components subject to wear.
Improperly executed and unauthorized repairs.
Unauthorized opening of the device or its components.
Catastrophic events due to the effect of foreign bodies or acts of God.
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USB-MEA256-System Manual
2.3 Important Safety Advice
Warning: Make sure to read the following advice prior to installation or use of the device and
the software. If you do not fulfill all requirements stated below, this may lead to malfunctions
or breakage of connected hardware, or even fatal injuries.
Warning: Always obey the rules of local regulations and laws. Only qualified personnel should
be allowed to perform laboratory work. Work according to good laboratory practice to obtain
best results and to minimize risks.
The product has been built to the state of the art and in accordance with recognized safety
engineering rules. The device may only
be used for its intended purpose;
be used when in a perfect condition.
Improper use could lead to serious, even fatal injuries to the user or third parties and damage
to the device itself or other material damage.
Warning: The device and the software are not intended for medical uses and must not
be used on humans.
Malfunctions which could impair safety should be rectified immediately.
High Voltage
Electrical cords must be properly laid and installed. The length and quality of the cords must
be in accordance with local provisions.
Only qualified technicians may work on the electrical system. It is essential that the accident
prevention regulations and those of the employers' liability associations are observed.
Each time before starting up, make sure that the power supply agrees with the specifications
of the product.
Check the power cord for damage each time the site is changed. Damaged power cords should
be replaced immediately and may never be reused.
Check the leads for damage. Damaged leads should be replaced immediately and may never
be reused.
Do not try to insert anything sharp or metallic into the vents or the case.
Liquids may cause short circuits or other damage. Always keep the device and the power cords
dry. Do not handle it with wet hands.
Requirements for the installation
Make sure that the device is not exposed to direct sunlight. Do not place anything on top of the
device, and do not place it on top of another heat producing device, so that the air can circulate
freely.
Warning: The device must not get in contact with fluids! Spilled liquid can damage or even
completely destroy the electronics of the amplifier! This is eminently important when using
a perfusion system. Take care that the flow rates of the inlet and outlet flow match so that
flooding of the amplifier is efficiently prevented.
4
3 Software Installation
3.1 Software Installation
Please check the system requirements before you install the MC_Rack software. MCS cannot
guarantee that the software works properly if these requirements are not fulfilled. Please see
the MC_Rack Help or Manual for more information. It is recommended that you check the
MCS web site for software updates on a regular basis.
The USB-MEA256-System is a plug and play device. The driver is automatically installed together
with the MC_Rack program. It is easier to connect the USB-MEA256-System first to the data
acquisition computer and then install MC_Rack.
Important: Please make sure that you have full control over your computer as an administrator.
Otherwise, it is possible that the installed hardware does not work properly.
3.1.1 System Requirements
Software: One of the following Windows ® operating systems is required: Windows 7, VISTA or XP
(English and German versions supported) with the NT file system (NTFS). Other language versions
may lead to software errors.
Hardware (Not required for offline analysis or demo mode): The data acquisition board USB-
MEA256-System. If no USB-MEA256-System is present, MC_Rack opens in a simulation mode.
A computer with low performance may lead to performance limits more often; therefore,
MCS recommends an up-to-date computer optionally with a separate hard disk. Please note
that there are sometimes hardware incompatibilities of the USB-MEA256-System and computer
components; or that an inappropriate computer power supply may lead to artifact signals.
Please contact your local retailer for more information on recommended computer hardware.
Important: You need to have installed the latest USB-MEA256-System driver to operate the
USB-MEA256-System, which is automatically installed with MC_Rack. The installation may
be invalid if the USB-MEA256-System does not respond. Please contact Multi Channel Systems
or your local retailer in this case.
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USB-MEA256-System Manual
3.1.2 Recommended BIOS settings
Recommended operating system settings
The following automatic services of the Windows operating system interfere with the data
storage on the hard disk and can lead to severe performance limits in MC_Rack. These routines
were designed for use on office computers, but are not very useful for a data acquisition
computer.
1. Turned off Windows System Restore.
2. Windows Indexing Service deselected for all local disks.
3. Optimize hard disk when idle (automatic disk fragmentation) turned off.
4. It is not recommended to run any applications in the background when using MC_Rack.
Remove all applications from Autostart folder.
5. Be careful when using a Virus Scanner. These programs are known to disturb MC_Rack,
and even data loss may occur.
6. When using an USB-MEA256-System it is recommended to connect a high performance
computer with a separate hard disk for program files and data storage. The provided
possibility to use up to 252 channels with a sample rate of up to 40 kHz needs high
memory capacity. Please remove data and defragment the hard disk regularly to ensure
optimal performance.
3.1.3 Driver Installation
The USB-MEA256-System is a plug and play device. The driver is automatically installed together
with the MC_Rack program. It is easier to connect the USB-MEA256-System first to the computer
and then install MC_Rack.
6
3.1.4 First Use of MC_Rack
It is also not recommended to run any applications in the background when using MC_Rack.
Remove all applications from the Autostart folder.
Warning: The operating system settings of the data acquisition computer were preconfigured
by MCS and should not be changed by the user. Changing these settings can lead to program
instabilities and data loss.
Installing MC_Rack with the USB-MEA256-System connected to the computer
The Windows operating system detects a new hardware when the USB-MEA256-System
is connected to the computer, if the program has not been installed beforehand. Please
make sure the device has power, that is, the power LED is lighting. Simply cancel the
"Found New Hardware Wizard" and proceed with the installation of the MC_Rack program.
Connecting the USB-MEA256-System to a computer with installed MC_Rack
If MC_Rack had already been installed on the computer before the hardware was connected,
the operating system needs to load the driver once. After this procedure, the USB-MEA256-
System will be automatically recognized by the operating system.
Software Installation
1. Connect the USB-MEA256-System to the USB port of the data acquisition computer.
Please make sure the device has power, that is, the power LED is lighting.
2. Switch the computer on.
The "Found New Hardware Wizard" is displayed.
3. Choose the option "No", not this time and continue with "Next".
4. Choose the option "Install" the software automatically ("Recommended")
and continue with "Next".
5. The Wizard will automatically find the appropriate USB-MEA256-System driver.
Continue with "Next".
6. The driver installation is finished.
Please check the system requirements before you install the MC_Rack software. MCS cannot
guarantee that the software works properly if these requirements are not fulfilled. Please see
the MC_Rack help or manual for more information. It is recommended that you check the
MCS web site for software updates on a regular basis.
Double-click Setup.exe on the installation volume.
The installation assistant will show up and guide you through the installation procedure.
7. Follow the instructions of the installation assistant.
8. The USB-MEA256-System driver and MC_Rack are installed (or updated) automatically.
7
4 USB-MEA256-System
4.1 The USB-MEA256 Data Acquisition
Analog input signals are acquired from the data source and digitized by the 256-channel analog /
digital converter that is integrated into the main unit. Recorded signals are converted in real-time
into digital data streams at sampling rates of up to 40 kHz per channel. You will not miss even the
fastest biological signals. Data is transferred to the computer via a High Speed USB 2.0 port.
A 16 bit digital (TTL) input / output channel is available. You can use the digital TTL inputs,
for example, for synchronizing stimulation and recording, or for synchronizing the USB-MEA256-
System with other systems, video tracking, for example. The digital TTL outputs can be used for
triggering other devices.
4.2 The USB-MEA256 Filter Amplification
The filter amplifier combines a band pass filter and the signal amplification in one instrument.
The bandwidth of 1 to 5000 Hz is suitable for a broad range of applications, such as spike and
field potential recording from neurons or recording of cardiac signals. The digital filter of the
MC_Rack program can be used to adjust the pass band and filter the raw data. Please see the
MC_Rack help or manual for more information. This way, you are very flexible in designing your
experiments. Please note that you may need a higher sampling rate to avoid aliasing. See also the
chapter USB-MEA256 Data Acquisition for more information. For slow signals like field potentials,
a bandwidth of 1 to 300 Hz is appropriate. If you like to record fast signals like spikes, a pass band
of 300 Hz to 5 kHz is suitable. Cardiac signals have fast and slow components; therefore, you
usually need a wider bandwidth of 1 Hz to 5 kHz.
Please note that the gain factor of the filter amplifier (1100) is a fixed hardware property; and
that you cannot change the gain of the amplifier by software controls. Please also note that the
ratio of the output signal to the input signal, that is, the gain, is not a fixed parameter for the
complete bandwidth. The gain that was specified for the amplifier, for example, 1100 is not fully
reached at the borders of the amplifier's pass band. The general rule is, that at the lower and
upper limit of the frequency band, the gain is approximately 70 % of the full gain. Therefore,
you should use a bandwidth that is at a safe distance of the signals of interest. Outside the pass
band, the gain decreases with the frequency and finally approaches zero.
For more information on gain and filters in general, please refer to standard literature or contact
your local retailer.
Raw data from up to 252 electrodes of a microelectrode array MEA is amplified by 252 channels
of filter amplifiers that are built very small and compact using SMD (Surface Mounted Devices)
technology. The small-sized MEA amplifier combines the interface to the 256MEA probe with the
signal filtering and the amplification of the signal. The compact design reduces line pick up and
keeps the noise level down. The MEA sensor is placed directly into the small-sized MEA amplifier.
When the amplifier is closed, the contact pins in the lid of the amplifier are pressed onto the MEA
contact pads. The very close location of the amplifier to the MEA sensor is very favorable
concerning a high signal-to-noise ratio.
The amplifier is intended to be used either with inverted or with upright microscopes.
The MEA256 amplifier is compatible to most standard microscopes.
The MEA256 amplifier has an integrated heating system for controlling the MEA's temperature.
The desired temperature can be easily regulated with a temperature controller TCX.
Connect the internal heating element to a temperature controller's output channel (D-Sub9
socket) with the integrated cable. Do not connect the black heating element cable to the
computer!
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USB-MEA256-System Manual
If necessary, you can use a Faraday cage or appropriate materials, for example, aluminum foil,
for shielding the amplifier. The shielding should be connected to the amplifier's ground.
Warning: Spilled liquid can damage or even completely destroy the electronics of the
MEA256 amplifier. Please be careful when setting up your perfusion system and when
starting the perfusion. Take care that the flow rates of the inlet and outlet flow match
so that flooding of the amplifier is efficiently prevented.
4.3 256MEA
The microelectrode array 256MEA to be used with the USB-MEA256-System contain 252
recording and four ground electrodes on a glass carrier. Contact to the amplifier is provided by a
double ring of contact pads around the rim of the MEA. The electrodes are from Titanium Nitride
(TiN) with a Silicon Nitride (SiN) isolator, and contact pads and tracks are made of transparent
Indium Tin Oxide (ITO). The electrode grid is 16 x16 with a spacing of 30, 60, 100 or 200 μm
between the electrodes with a diameter of 8, 10 and 30 μm. The electrode diameter of 30 μm
results in an impedance of approximately 30 - 50 k. Smaller electrodes have a higher impedance,
so the electrode diameter of 10 or 8 μm results in an impedance of approximately 250 - 400 k.
The dimension of the glass carrier is 49 x 49 x 1 mm. MEAs are stable in a temperature range from
0° - 125° C. For information about handling and cleaning, please refer to the MEA manual and / or
to the MEA Cleaning Quick Guide. The Pin Layout is described in chapter “Data Sheet 256MEA”
in the Appendix. The 256MEA is rotationally symmetrical, so in principle the orientation in the
amplifier doesn’t matter. If the orientation is important for your experiments, you can use the
engraved serial number as marker. The serial number is on the backside of the MEA in the upper
right edge. In the amplifier the mirrored serial number has to be placed in the upper left edge.
This way the 256-electrode layout matchs with the MC_Rack channel layout.
10
4.4 256 Thin MEA
The glass part of 256 Thin MEAs are only 180 μm "thick", ideally suited for high-resolution
imaging. 256ThinMEAs are like standard MEAs, but the electrodes are embedded in a very
thin and delicate glass substrate on a robust ceramic carrier. The thin glass allows the use
of oil immersion objectives with a high numerical aperture.
Like 256MEAs, 252 electrodes are arranged in a 16 x 16 layout grid with electrode diameter
of 30 μm, and interelectrode distance of 200 μm (256ThinMEA200/30-ITO).
256ThinMEAs are heat-stabilized and can be autoclaved. They can also be coated with different
procedures for cell and tissue cultures. They should be handled with great care because of the
thin and delicate recording area. Please see chapter "Data Sheet 256ThinMEA" in the Appendix.
4.5 256-9well MEA
USB-MEA256-System
A special MEA layout for the USB-MEA256-System is available, the 256-9wellMEA for
using together with a 9 well macrolon quadrate. The 9 wells allow, for example drug screening
experiments, with up to 9 compounds at a time. The 256-9wellMEA is in principle constructed
like the 256MEA, but the electrodes are clustered. There are 23 recording electrodes with
a diameter of 30 μm, two bigger recording or stimulation electrodes (2 x 50 μm), and one
big reference electrode per each well. Please see chapter "Data Sheet 256-9wellMEA"
in the Appendix.
ONNECTING THE USB-MEA256-SYSTEM
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USB-MEA256-System Manual
4.6 The USB-MEA256-System
USB-MEA256-System is a high flexible system with integrated amplification, data acquisition,
and analog / digital conversion. Via USB High Speed 2.0 it is possible to transfer a digitally
converted and amplified data stream of up to 252 electrode channels to any data acquisition
computer.
Note: Using a USB hub for connecting the USB-MEA256-System to the computer is not
recommended. The system needs a broad bandwidth for the data transmission. Recording
might not be possible, especially if a second device that sends or receives continuous data
streams, for example a web cam or USB speakers, is connected to the same USB port.
Top View
4.7 Connecting the USB-MEA256-System
1. Provide a power supply in the immediate vicinity of the installation site.
2. Place all devices on a stable and dry surface, where the air can circulate freely and the devices
are not exposed to direct sunlight.
3. Set up the computer (with installed MC_Rack program).
4. Set up the MEA256 amplifier as described in chapter "Setting up the Amplifier".
5. Connect the USB output connector to a free USB 2.0 port of the data proceeding computer.
It is not recommended to use an USB hub.
6. Connect the USB-MEA256 via power supply unit to a power outlet of the same electrical system
(connected to the same ground / earth wire) as all other components of the setup, for example,
the computer or shielding.
7. If necessary, connect the system to an external ground.
8. Connect the internal heating element to the temperature controller's output channel (D-Sub9
socket) with the integrated cable. Do not connect the heating element cable to the computer!
9. Turn the toggle switch at the rear panel on.
10. Check the power LED. It should light up as soon as the power line is connected, and the toggle
switch is switched on. If not, check the power source and cabling.
11. Install the MC_Rack program from the installation volume if it is not already installed.
The USB-MEA256-System is a plug and play device. The driver is automatically installed
together with the MC_Rack program.
12. Start the MC_Rack program and select the USB-MEA256-System as the data source.
Please see the MC_Rack help or manual for more details on how to define the data source.
12
4.8 Setting Up the Amplifier
Open the lid of the amplifier.
USB-MEA256-System
Place the 256MEA test model probe inside. Replace the lid and close it carefully.
First Functional Tests
Each MEA amplifier has been thoroughly tested at the factory site before delivery. However,
you may want to perform some tests yourself before you begin your experiment to exclude
any damage that might have occurred during transportation, or to fulfill your own guidelines,
for instance. Some of the tests will also help you to get to know the basic functions of the hardand software, like a short tutorial. It will take only a few minutes time and can save you time and
trouble in the long run. Multi Channel Systems recommends running these tests after the setup of
your system before you start your real experiments.
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USB-MEA256-System Manual
General Performance / Noise Level
Please use the provided test model probe to test the amplifier immediately after installation.
It simulates a MEA with a resistor of 220 k and a 1 nF capacitor between bath and electrode.
Use MC_Rack or your custom data acquisition program to record from the test model probe and
to check the amplifier. Please see "Test Model Probe" in the Appendix.
Alternative to the test model probe you can use n 256MEA Signal Generator. 256MEA Signal
Generator is a convenient tool for USB-MEA256 System users. Use the 256MEA-SG instead of
setting up an experiment with biological sample for training, controlling, and troubleshooting
purposes. This reduces the number of animal experiments and saves laboratory equipment.
Please see the data sheet "256MEA-SG" in the Appendix
Setting up MC_Rack
Please refer to the MC_Rack manual for more information.
1. Start MC_Rack.
2. Click “Data Source Setup” on the “Edit” menu. Select USB-MEA256.
3. Add USB-MEA256 as a data source to your virtual rack.
4. On the “Edit” menu, click “Add Data Display” to add a raw data display to your virtual rack.
5. Click the “Hardware” tab of the USB-MEA256 and enter the sampling frequency in Hz.
Start the data acquisition
1. Click the “Start” button to start the data acquisition. No data is recorded. You see the raw data
streams of all 252 channels in the 16 x 16 MEA layout.
2. You may have to adjust the position and span of the axes until you can clearly see the noise level.
You should see the baseline with a maximum noise level of +/– 8 μV.
14
USB-MEA256-System
The following screen shot shows a recording from a USB-MEA256-System with a test model
probe and a sampling rate of 40 kHz.
Setting up an Experiment
Open the lid of the amplifier. Place the 256MEA chip inside the USB-MEA256 amplifier.
The pin layout is described in chapter “Pin Layout” in the Appendix. The MEA256 is rotationally
symmetrical, so in principle the orientation in the amplifier doesn’t matter. If the orientation
is important for your experiments, you can use the engraved serial number as marker.
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USB-MEA256-System Manual
Replace the lid and close it carefully.
Warning: Spilled liquid can damage or even completely destroy the electronics of the
MEA256 amplifier. Please be careful when setting up your perfusion system and when
starting the perfusion. Take care that the flow rates of the inlet and outlet flow match
so that flooding of the amplifier is efficiently prevented.
Grounding the bath
256MEAs are equipped with 4 internal reference electrodes, which will be automatically
connected to the systems ground when the amplifier is closed. This has the advantage that
you can keep the culture chamber closed and sterile (for example, with MEA-MEM
semipermeable membranes).
Service and Maintenance
You should clean the contact pins of the amplifier carefully with a soft tissue and alcohol or
isopropanol from time to time, especially if you have problems with the noise level. For handling
and cleaning of the MEA, please refer to the MEA manual or to MEA Cleaning Quick Guide on the
MCS web site www.multichannelsystems.com.
Warning: Please be very careful when handling the amplifier, or cleaning the device!
The pins can be damaged easily.
16
USB-MEA256-System
Using 256MEA Electrodes for Stimulation
The electrodes of the 256MEAs can also be used for electrical stimulation. Around the open area
for the 256MEA, there are two rows of connection sockets on each side in the lid of the amplifier.
There is one socket for each electrode and four ground sockets. These sockets can be used to
connect each electrode to a stimulus generator, for example, a STG4000 from Multi Channel
Systems MCS GmbH. The ground sockets can be used to connect other devices, like the stimulator,
or the perfusion to the systems ground. You will find the layout map of the sockets in chapter
"Pin Layout" in the Appendix.
To make it easier to find the correct socket for each electrode, stickers are included to color code
the electrode sockets in four blocks of 2 x 8 sockets, corresponding to the color code used in the
layout map in chapter "Pin Layout" in the Appendix. If you want to do electrical stimulation,
please attach the stickers as shown in the image below. The edge of the sticker has to be aligned
with the first pair of sockets.
The position of the four ground sockets is labeled with a G. The connection sockets are arranged
in quadrants. The electrodes in quadrant 1 have their sockets in the upper row, electrodes in
quadrant 2 in the right row and so on.
To find the connection socket for a specific electrode, please do following:
Determine the quadrant the electrode is in.
Look up the exact position of the socket in the layout map .
Determine the label (yellow/grey) the socket belongs to.
Count the sockets from the edge of the label.
Plug in the stimulation adapter ADPT-STIM-MEA256 in the correct connector socket.
Connect the ADPT-STIM-MEA256 via laboratory cable with the stimulus generator STG.
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USB-MEA256-System Manual
Using 256-9wellMEA Electrodes for Stimulation
Each well of the nine wells of the 256-9wellMEA is equipped with 26 round recording electrodes
in a 6 x 5 grid, two square electrodes S1 and S2 for recording or stimulation and one big reference
electrode. For detailed information, please read chapter “256-9wellMEA” and the data sheet.
If stimulation electrodes S1 and S2 in all wells are to be stimulated simultaneously,
it is recommended to use the adapter 256MEA-9well-STIM-ADPT. See also the data sheet
”256MEA-9well-STIM-ADPT” in the Appendix.
Please fix the adapter 256MEA-9well-STIM-ADPT on the connector sockets on the lid of the
USB-MEA256 amplifier as shown on the picture above. The red connectors (2 mm) connect
to the stimulation electrodes S1 or S2 in each well, respectively. The black connectors connect
to the ground electrodes in each well.
Three modes of stimulation are possible:
Monopolar S1 versus ground: Connect the stimulator output to the red connector S1,
and the stimulator ground to the black connector S1.
Monopolar S2 versus ground: Connect the stimulator output to the red connector S2,
and the stimulator ground to the black connector S2.
Bipolar S1 versus S2: Connect the positive stimulator output to the red connector S1,
the negative stimulator output to the red connector S2, and the stimulator ground
to the black connector S1 or S2.
Please see STG manual for details on stimulation modes.
18
4.9 The USB-MEA256 Device
4.9.1 Rear Panel
DIGITAL IN / OUT
A Digital IN / OUT for 16 digital in- and output bits is available (68-pin MCS standard connector).
The Digital IN / OUT connection accepts or generates standard TTL signals. TTL stands for
Transistor-Transistor Logic. A TTL pulse is defined as a digital signal for communication between
two devices. A voltage between 0 V and 0.8 V is considered as a logical state of 0 (LOW), and
a voltage between 2 V and 5 V means 1 (HIGH).
The Digital OUT allows generating a digital signal with up to 16 bits and read it out, for example,
by using a Digital IN / OUT Extension Di/o from Multi Channel Systems MCS GmbH. You can
utilize this digital signal to control and synchronize other devices with the USB-MEA256-System.
Bit 0 of the Digital OUT is separated and available as Lemo connector DIG OUT D0. So if you need
only one bit of the digital signal, you don’t need the additional signal divider SD16. Please read
chapter "Pin Layout" (Digital IN / OUT Connector) in the Appendix for more information.
USB-MEA256-System
The Digital IN can be used to record additional information from external devices as a 16-bit
encoded number. The Digital IN is most often used to trigger recordings with a TTL signal from
a stimulator.
The 16 bit digital input channels is a stream of 16-bit values. The state of each bit (0 to 15) can
be controlled separately, the state can be HIGH (1) or LOW (0). Standard TTL signals are accepted
as input signals on the digital inputs. Unused input bits, which have an undefined state, should
be masked in the Trigger Detector of MC_Rack.
Warning: A voltage that is higher than +5 Volts or lower than 0 Volts, that is, a negative
voltage, applied to the digital input would destroy the electronics. Make sure that you apply
only TTL pulses (0 – 5 V) to the digital inputs.
Power LED
The Power LED lights up when the USB-MEA256 is connected via power supply unit
to the power supply system, and the toggle switch on the rear panel is switched on.
Digital Output D0 OUT
The Bit 0 of the Digital OUT is also accessible independently from the 68-pin Digital IN / OUT
connector with a Lemo connector. The digital output channel D0 is generally used for
synchronizing the USB-MEA256-System with a stimulus generator, or with another data
acquisition system, for example, an imaging or a patch clamp system. The D0 OUT generates
standard TTL pulses.
Digital Input D0 IN
The Bit 0 of the Digital IN is also accessible independently from the 68-pin Digital IN / OUT
connector with a Lemo connector. The digital output channel D0 is generally used for
synchronizing the USB-MEA256-System with a stimulus generator, or with another data
acquisition system, for example, an imaging or a patch clamp system. The D0 IN accepts standard
TTL pulses.
19
USB-MEA256-System Manual
Audio Output AUDIO
To the AUDIO OUT (3.5 mm phone jack) you can connect an audio system to make the electrical
activity audible. In contrast to the sound tool of MC_Rack, this audio output is real time. There
is almost no time delay between the detection of a signal by the recording electrode and the
corresponding sound. Headphones or a speaker can be connected directly to the AUDIO OUT.
Only one channel can be converted into sound. This channel can still be selected by using the
sound instrument of MC_Rack. You can listen only to Electrode Raw Data in real time. The quality
of the sound is mono.
Note: You can add only one sound instrument to your rack, and you can convert only one channel
into sound.
Analog Input A1 to A4
Four additional analog inputs are available (Lemo connectors).
The amplifier delivers analog data streams from 252 channels to the integrated data acquisition
and A / D converter of the USB-MEA256. The remaining four channels 127, 128, 255 and 256 are
used for the additional analog inputs Analog IN A1 to A4.
The additional analog inputs one to four are intended for recording additional information
from external devices, for example, for recording patch clamp in parallel to the MEA recording,
for monitoring the temperature, or for recording voice. You could also use the analog inputs
for triggering, but please note that the digital inputs, especially D0 IN are intended for accepting
TTL pulses. Signals on the analog channels are not amplified. They are only digitized and recorded
as they are, with no respect to the gain specified in MC_Rack.
USB
The USB connector is used to transfer the amplified and digitized data from all data channels
and the additional digital and analog channels to any connected data acquisition computer via
High Speed USB 2.0 (type A - mini B) cable.
GROUND
If an additional ground connection is needed, you can connect this plug with an external ground
using a standard common jack (4 mm).
POWER IN
Connect the power supply unit here. This power supply powers the USB-MEA256 main unit only.
The device needs 12 V and 1.3 A / 16 W.
Toggle Switch I / O
Toggle switch for turning the device on and off. The USB-MEA256 is switched to status "ON"
when the toggle switch is switched to the left to "I". The device is switched "OFF" when the
toggle switch is switched to the right "O". If the USB-MEA256 is "ON", and the device is
connected to the power line, the LED should light up. If not, please check the power source
and cabling.
20
USB-MEA256-System
Cabling
For D0 IN and D0 OUT, and Analog Input A1 to A4 on the rear panel of the device you need
coaxial cable: Lemo cable with one head BNC connector, the other head "Lemo" connector.
Please see the picture below.
Temperature Controller TC01 / 02
The USB-MEA256-System features a heating element with a PT100 temperature sensor.
If you connect a temperature controller TCX to the heating element via D-Sub 9 connector,
the heating element guarantees constant temperature conditions for the biological sample,
placed on the 256MEA.
21
5 Troubleshooting
5.1 Troubleshooting
The following hints are provided to solve special problems that have been reported by users.
Most problems occur seldom and only under specific circumstances. Please check the mentioned
possible causes carefully when you have any trouble with the product. In most cases, it is only
a minor problem that can be easily avoided or solved.
If the problem persists, please contact your local retailer. The highly qualified staff will be glad
to help you. Please inform your local retailer as well, if other problems that are not mentioned
in this documentation occur, even if you have solved the problem on your own. This helps other
users, and it helps MCS to optimize the instrument and the documentation. Please pay attention
to the safety and service information in the separate manuals of the related products and in the
software help. Multi Channel Systems has put all effort into making the product fully stable
and reliable, but like all high-performance products, it has to be handled with care.
5.2 No Computer Connection / No Recording Possible
You cannot establish a connection to the computer. The USB-MEA256-System channel layout
is not available in MC_Rack. When loading a previously saved virtual rack file, you will get an error
message and the simulator will be started automatically. You get an error message when starting
the recording in MC_Rack after a successful computer connection.
Possible causes:
? The power LED is not lightning. The supply power is not connected or there is a technical
problem with the instrument.
Check the power source and the cable connections. If this does not solve the problem, contact
your local retailer for support.
? The power LED is lighting. The USB port might not support USB 2.0 or might not be working.
Check the USB port. Only full speed USB 2.0 ports can be used. Try another USB 2.0 port.
? You can establish a connection, but get an error message when starting the recording
in MC_Rack. The bandwidth of the USB port is not sufficient for recording. This can be the
case if the USB-MEA256-System is connected via USB hub, and a second device that sends
or receives continuous data streams, for example, a web cam or USB speakers, is connected
to the same USB port.
Connect the USB-MEA256-System directly to a USB port, not via hub.
23
USB-MEA256-System Manual
5.3 Triggering / Digital Input does not Work
You have connected a TTL source (for example, the Sync Out of a stimulus generator) to the
digital input D0 of the USB-MEA256-System, and configured the virtual rack in MC_Rack
for triggering displays or data acquisition by the TTL source, but you do not see any sweeps.
Possible causes:
? The TTL source does not generate true TTL signals (5 V TTL level), or the TTL pulse duration
is too short in combination with the sampling rate, so that the pulse is missed in-between two
data points.
The USB-MEA256-System can only accept TTL signals (5 V TTL level) as a digital input stream.
The TTL pulse needs to be optimized according to the sampling rate. Otherwise, a detection
of the trigger cannot be guaranteed.
? The software settings for the Trigger Detector do not match with the hardware configuration.
In MC_Rack, add a Trigger Detector to your virtual rack, and select the Digital Data D1 input
stream as the Trigger. Check the pin layout of the Digital IN/OUT connector and make sure that
the same bit input that is connected is selected in the software. (The standard settings of the
Trigger Detector are for using bit 0.) Mask all unused bits. Select the appropriate logical state
(generally HIGH) for triggering. Please see the MC_Rack help or manual for more details.
5.4 Noise on Single Electrodes
The noise level on single electrodes is significantly higher than expected or you see artifact signals.
Possible causes:
? The electrode or the contact pin of the amplifier may be defective. To test this, do the following.
Open the amplifier and turn the 256MEA by 90 degrees. Close the amplifier again and start
the recording.
If the same electrode in the MEA layout is affected, the amplifier's contact is not okay.
If another electrode is now affected and the previously affected electrode is okay now,
the 256MEA electrode is not okay, but the amplifier is fine.
— OR —
Use the test model probe to test the amplifier. If the noise level is fine without the MEA,
bad MEA electrodes cannot be the cause.
24
5.5 MEA is defective
MEAs wear out after multiple uses or over a longer time of use, for example, for long-term
cultures. This is considered a normal behavior. MEAs are also easily damaged by mishandling,
for example, if wrong cleaning solutions or too severe cleaning methods are used or if the
recording area is touched. If you observe a bad long-term performance of MEAs, consider
a more careful handling.
Possible causes:
? The contact pads are contaminated.
Clean the contact pads carefully with a swab or a soft tissue and pure (100 %) alcohol.
? The contact pads or the electrodes are irreversibly damaged. You could have a look at the
electrodes under a microscope: If they appear shiny golden, the TiN is gone and the electrode
is irreversibly damaged. Electrodes may be damaged without changing their visual appearance,
though.
Pick one of the bad channels after the other and ground it. See the MEA amplifier's manual for
more information on grounding channels. In most cases, only one of the electrodes that appear
bad is actually defective, and the other ones are only affected by the single defective electrode.
Ground as many electrodes as you need for a good general performance. Grounded electrodes
show a noise level that is lower than that of good electrodes. If too many electrodes are defective,
use a new MEA.
Troubleshooting
5.6 Overall Noise / Unsteady Baseline
The baseline is unstable, signals are jumping or drifting.
Possible causes:
? Bath electrode is not connected to ground.
Connect the internal or external bath electrode to one of the ground inputs of the amplifier.
? AgCl bath electrode is not well-chlorided.
Rechloride the electrode or use a new one.
? 50 Hz hum: 50 Hz is the frequency of mains power in Europe. If the shielding and grounding
of the setup is not sufficient, electrical signals are picked up from the environment.
Use a proper shielding. For example, you can place aluminum foil over the amplifier that
is connected to any metal part of the MEA amplifier. You can also use special shielding
equipment like a Faraday cage.
25
USB-MEA256-System Manual
5.7 Missing Spikes or Strange Signal Behavior
MEAs wear out after multiple uses or over a longer time of use, for example for long-term
cultures. The insulation layer gets thin over time. This is considered a normal behavior.
Possible causes:
? The insulation layer is too thin. As a result, the MEA gets the behavior of a low pass filter. This
means, that the signal frequency may be shifted to a lower frequency, and spikes are missing.
Optically control the MEA with a microscope. If concentric colored rings (Newton rings) are visible
(due to light interference), the insulation layer is too thin and you should use a fresh MEA.
? The insulation layer has been abraded and is missing in parts. This will result in a short circuit
between the electrode/tracks and the bath. You will still see signals, but as an unspecific smear
over the complete array.
Use a fresh MEA.
26
6 Appendix
6.1 Technical Support
Please read the chapter "Troubleshooting" of the user manual first. Most problems are caused
by minor handling errors. Contact your local retailer immediately if the cause of trouble remains
unclear. Please understand that information on your hardware and software configuration
is necessary to analyze and finally solve the problem you encounter. Please keep information
on the following at hand:
Description of the error (the error message text or any other useful information) and of the
context in which the error occurred. Try to remember all steps you had performed immediately
before the error occurred. The more information on the actual situation you can provide,
the easier it is to track the problem.
The serial number of the device. You will find it on the backside of the housing or in MC_Rack
Hardware tab.
The operating system and service pack number of the connected computer.
The hardware configuration (microprocessor, frequency, main memory, hard disk) of the
connected computer. This information is especially important if you have modified the computer
or installed new hard- or software recently.
The version of the recording software. On the "Help menu", click "About" to display the software
version.
27
USB-MEA256-System Manual
6.2 Technical Specifications
The USB-MEA256-System is a 252 + 4 channels amplifier with integrated analog / digital board
converting analog signals to digital data streams in real time. It is intended to directly contact
to a 252 electrode Microelectrode Array (MEA).
General characteristics:
Operating temperature 10 °C to 50 °C
Storage temperature 0 °C to 70 °C
Relative humidity 10 % to 85 %, non-condensing
Dimensions (L x D x H) 325.4 mm x 207.7 mm x 25.2 mm
Weight 2005 g
Amplifier:
Number of electrode channels 252
Input voltage range +/- 3.7 mV
Data resolution 16 Bit
Bandwidth 1 Hz – 5 kHz
Gain of analog signal path 1100
Cross talk (channel to channel) typical 0.01 %, max. 0.1 %
Additional analog inputs:
Number of additional inputs 4
Input voltage range +/- 4.096 V
Bandwidth DC to 15 kHz
Input impedance
DC offset max. +/- 2 mV
Digital inputs & outputs
Number of digital input channels 16
Digital input signal levels CMOS (3.3 V input)
Digital input impedance
Number of digital output channels 16
Digital output signal levels CMOS (3.3 V output)
1 M || 1 nF
100 k
28
Appendix
Data converter and USB interface:
Sampling frequency up to 40 kHz
Maximal data rate 20.5 MB/s
Data resolution 16 Bit
USB Version USB 2.0 High Speed
Heating element and temperature
sensor:
Heating element impedance
20
Temperature sensor type PT 100 with 4 wire connection
Rear Panel interface and connectors:
1 16 bit digital in / out 68-pin MCS standard connectors,
MCS high grade cable
1 Digital out D0 OUT Lemo connector (EPL.00.250 NTN)
1 Digital in D0 IN Lemo connector (EPL.00.250 NTN)
1 Audio output Stereo jack 3.5 mm
4 Additional analog inputs Lemo connector (EPL.00.250 NTN)
USB USB 2.0 High Speed cable (type A – Mini B)
Ground Common jack 4 mm, banana plug
Power supply Barrel connector 0.7 x 2.35 mm
Power supply unit (MPU 30):
Input voltage 90 – 264 VAC @ 47 - 63 Hz
Output voltage 11 – 13 V
Max. Power 30 W
Software:
MC_Rack program Version 3.7.0 and higher
Operating system Windows 7, XP or Vista with NTFS
English and German versions are supported
MC_DataTool program Version 2.4.5 and higher
Data export ASCII (*.txt), binary file (*.raw) format
Warning: The device may only be used together with Microelectrode Arrays from Multi
Channel Systems MCS GmbH, and only for the specified purpose. Damage of the device and
even fatal injuries can result from improper use. Do not open the data acquisition box and
do not change hardware configuration as it could lead to improper behavior of the system.
29
USB-MEA256-System Manual
6.3 Pin Layout
Analog IN A1 to A4
Please note that the channels 127, 128, 255 and 256 are not used for recording analog
raw data from 256MEA in the USB-MEA256-System. A1 to A4 are for additional analog data.
Analog 1 Channel 127
Analog 2 Channel 128
Analog 3 Channel 255
Analog 4 Channel 256
Digital IN / OUT Connector
68-Pin MCS Standard Connector
Pin 1 GNDP (power ground)
Pin 2 GNDS (signal ground)
Pin 3 - 10 Digital output channels bit 0 - 7
Pin 11 - 14 GNDS (signal ground)
Pin 15 - 22 Digital output channels bit 8 - 15
Pin 23 - 26 GNDS (signal ground)
Pin 27 - 34 Digital input channels bit 0 - 7
Pin 35 - 38 GNDS (signal ground)
Pin 39 - 46 Digital input channels bit 8 - 15
Pin 47 - 48 GNDS (signal ground)
Pin 49 - 63 Internal use (do not connect)
Pin 64 - 66 GNDS (signal ground)
Pin 67 Positive supply voltage output
Pin 68 Negative voltage supply output
Digital Out D0 OUT Bit 0 of the 16 bit digital output channels (Pin 3)
Digital In D0 IN Bit 0 of the 16 bit digital input channels (Pin 27)
30
USB-MEA256-System Amplifier: Stimulation Connector Sockets 1 to 4
Appendix
USB-MEA256-System Amplifier: Spring Contacts
Number of the spring contact in the lid of the amplifier which connect to the contact pads
of the 256MEA. The spring contacts are counted clockwise, starting in the upper left edge.
31
USB-MEA256-System Manual
6.4 Test Model Probe
The provided model test probe simulates a 256MEA with a resistor of 220 k and a 1 nF capacitor
between bath and electrode, for all 256 electrodes, and can be used for testing USB-MEA256Systems.
32
6.5 Data Sheet 256MEA
Appendix
33
256MEA
256MEA30/8iR-ITO
256MEA60/10iR-ITO
256MEA100/30iR-ITO
256MEA200/30iR-ITO
2
1
Connector 1
64
63
Serial Number
A B C D E F G H I K L M N O P R
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
A B C D E F G H I K L M N O P R
Connector 3
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
256 Microelectrode Array for
64
63
Connector 4
1
2
63
64
use with USB-MEA256-System.
Technical Specifications 256MEA
Temperature compatibility 0 - 125 °C
Dimension (W x D x H) 49 mm x 49 mm x 1 mm
Base material Glass
1
2
Connector 2
64
63
1
2
Contact pads and track material Indium tin oxide (ITO)
Electrode diameter 8, 10 or 30 μm
Interelectrode distance (centre to centre) 30, 60, 100 or 200 μm
Electrode height Planar
Electrode type Titanium nitride (TiN)
Isolation type Silicon nitride (SiN)
Electrode impedance 30 - 50 k for 30 μm, or 250 - 400 k for 10 μm
Electrode layout grid 16 x 16
Number of recording electrodes 252
Number of reference electrodes 4 internal reference electrodes (iR)
Contact pads for reference electrodes (connected to ground) 4
MC_Rack
Source layout in “Data Source Setup” Configuration
Channel map 16 x 16.cmp
MEA perfusion chamber (w/o) Without ring
(gr) Glass ring: ID +/- 19 mm, OD 24 mm, height 6 / 12 mm
(pr) Plastic ring without thread: ID 26.5 mm, OD 30 mm, height 6 / 3 mm
(pr-T) Plastic ring with thread: ID 26 mm, OD 30 mm, height 6 / 15 mm
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2014 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
256MEA
A 2 B 1 C 2 E 5 D 3 D 1 E 4 E 2 F 5 F 3 F 1 G 4 G 2 H 5 H 3 H 1 H 7 I 6 I 2 I 4 K 1 K 3 K 5 L 2 L 4 M 1 M 3 K 6 N 2 I 8 O 1 GND
B 2 C 3 C 1 G 7 D 2 G 6 E 3 E 1 F 4 F 2 G 5 G 3 G 1 H 4 H 2 H 6 I 7 I 1 I 3 I 5 K 2 K 4 L 1 L 3 L 5 M 2 M 4 N 1 N3 L 6 O 2 N 4
D 4
GND
B 3
A 3
H 8
F 6
B 4
C 4
A 4
F 7
C 5
A 5
D 6
B 6
E 7
C 7
A 7
D 8
B 8
F 8
G 9
A 9
C 9
E 9
B10
D10
A11
C11
E11
B12
D12
A13
C13
E12
B14
A15
B16
D 5
B 5
E 6
C 6
A 6
D 7
B 7
E 8
C 8
A 8
G 8
F 9
B 9
D 9
A10
C10
E10
B11
D11
A12
C12
F10
B13
G10
A14
C14
B15
B 1 C 1 D 1 E 1 F 1 G 1 H 1 I 1 K 1 L 1 M 1 N 1 O 1 P 1
A 2 B 2 C 2 D 2 E 2 F 2 G 2 H 2 I 2 K 2 L2 M 2 N 2 O 2 P 2 R 2
A 3 B 3 C 3 D 3 E 3 F 3 G 3 H 3 I 3 K 3 L 3 M 3 N 3 O 3 P 3 R 3
A 4 B 4 C 4 D 4 E 4 F 4 G 4 H 4 I 4 K 4 L 4 M 4 N 4 O 4 P 4 R 4
A 5 B 5 C 5 D 5 E 5 F 5 G 5 H 5 I 5 K 5 L 5 M 5 N 5 O 5 P 5 R 5
A 6 B 6 C 6 D 6 E 6 F 6 G 6 H 6 I 6 K 6 L 6 M 6 N 6 O 6 P 6 R 6
A 7 B 7 C 7 D 7 E 7 F 7 G 7 H 7 I 7 K 7 L 7 M 7 N 7 O 7 P 7 R 7
A 8 B 8 C 8 D 8 E 8 F 8 G 8 H 8 I 8 K 8 L 8 M 8 N 8 O 8 P 8 R 8
A 9 B 9 C 9 D 9 E 9 F 9 G 9 H 9 I 9 K 9 L 9 M 9 N 9 O 9 P 9 R 9
A10 B10 C10 D10 E10 F10 G10 H10 I10 K10 L10 M10 N10 O10 P10 R10
A11 B11 C11 D11 E11 F11 G11 H11 I11 K11 L11 M11 N11 O11 P11 R11
A12 B12 C12 D12 E12 F12 G12 H12 I12 K12 L12 M12 N12 O12 P12 R12
A13 B13 C13 D13 E13 F13 G13 H13 I13 K13 L13 M13 N13 O13 P13 R13
A14 B14 C14 D14 E14 F14 G14 H14 I14 K14 L14 M14 N14 O14 P14 R14
A15 B15 C15 D15 E15 F15 G15 H15 I15 K15 L15 M15 N15 O15 P15 R15
B16 C16 D16 E16 F16 G16 H16 I16 K16 L16 M16 N16 O16 P16
Serial Number
P 2
O 3
R 3
K 7
P 4
L 7
C 5
R 5
N 6
P 6
M 7
C 7
R 7
N 8
P 8
L 8
K 9
R 9
O 9
M 9
P10
N10
R11
O11
M11
P12
N12
R13
O13
L11
P14
N13
P 1
R 2
P 3
M 5
O 4
R 4
N 5
P 5
M 6
C 6
R 6
N 7
P 7
M 8
O 8
R 8
K 8
L 9
P 9
N 9
R10
O10
M10
P11
N11
R11
O12
L10
P13
I 9
R14
GND
D13 C15 F11 D14 D16 E13 E15 F12 F14 F16 G13 G15 H12 H14 H16 H10 I11 I15 I13 K16 K14 K12 L15 L13 M16 M14 K11 N15 K10 O16 O14 P15
GND C16 H 9 D15 G11 E14 E16 F13 F15 G12 G14 G16 H13 H15 H11 I10 I16 I14 I12 K15 K13 L16 L14 L112 M15 M13 N16 N14 M12 O15 P16 R15
The letter digit code is the electrode identifier, and refers to the position of the electrode in the 16 x 16 layout grid.
The layout of the letter digit code for the four connectors of the USB-MEA256 amplifier is shown. To correlate the
pin layout of the connectors, please see the table on the next page.
The MEA is rotationally symmetrical, so the orientation does not matter. If the orientation is important for your
experiments, you can use the engraved serial number as marker. The serial number is on the backside of the MEA
in the upper right edge. In the amplifier the mirrored number has to be placed in the left upper edge. This way
the 256-electrode layout will match the MC_Rack channel layout.
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2014 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
256MEA
Stimulation Connector Socket 3
Electrode
ID
Stim.
Socket
Spring
Contact
Hardware
ID
C15
61
98
239
C16
62
222
209
D13
63
99
171
D14
57
96
184
D15
58
220
213
D16
55
95
181
E13
53
94
182
E14
54
218
243
E15
51
93
179
E16
52
217
147
F11
59
97
183
F12
49
92
180
F13
50
216
145
F14
47
91
177
F15
48
215
191
F16
45
90
178
G11
56
219
245
G12
46
214
192
G13
43
89
175
G14
44
213
189
G15
41
88
176
G16
42
212
190
H10
33
84
150
H11
36
209
185
H12
39
87
153
H13
40
211
187
H14
37
86
152
H15
38
210
188
H16
35
85
154
H9
60
221
215
I 10
34
208
186
I 11
31
83
151
I 12
28
205
59
I 13
27
81
149
I 14
30
206
62
I 15
29
82
148
I 16
32
207
61
K10 7 71
63
K11
11
73
247
K12
21
78
250
K13
24
203
57
K14
23
79
251
K15
26
204
60
K16
25
80
146
L12
18
200
220
L13
17
76
248
L14
20
201
221
L15
19
77
252
L16
22
202
58
M12 8 195
217
M13
14
198
218
M14
13
74
246
M15
16
199
222
M16
15
75
249
N14
10
196
216
N15 9 72
244
N16
12
197
219
O14 3 69
28
O15 6 194
214
O16 5 70
64
P15 1 68
96
P16 4 193
126
R15 2 67
66
GND
64
255
Stimulation Connector Socket 4
Electrode
ID
Stim.
Socket
Spring
Contact
Hardware
ID
A10
25
113
236
A11
22
232
205
A12
15
108
169
A13
12
227
143
A14 5 103
241
A15 4 223
141
A3
62
252
195
A4
55
128
159
A5
52
247
131
A6
45
123
229
A7
42
242
202
A8
35
118
166
A9
32
237
138
B10
26
234
206
B11
19
110
170
B12
16
229
144
B13 9 105
242
B14 6 224
211
B15 1 101
240
B16 2 100
210
B3
61
131
225
B4
58
250
198
B5
51
126
162
B6
48
245
134
B7
41
121
232
B8
38
240
201
B9
29
115
233
C10
23
112
167
C11
20
231
139
C12
13
107
237
C13
10
226
212
C14 3 102
172
C4
57
129
228
C5
54
248
197
C6
47
124
161
C7
44
243
133
C8
37
119
231
C9
30
236
203
D10
24
233
140
D11
17
109
238
D12
14
228
207
D4
63
132
157
D5
53
127
227
D6
50
246
200
D7
43
122
164
D8
40
241
136
D9
27
114
168
E10
21
111
235
E11
18
230
208
E12 8 225
142
E6
49
125
230
E7
46
244
199
E8
39
120
163
E9
28
235
137
F10
11
106
174
F6
59
130
160
F7
56
249
132
F8
36
239
135
F9
31
116
165
G10 7 104
173
G8
33
117
234
G9
34
238
204
H8
60
251
129
GND
64
256
Stimulation Connector Socket 2
Electrode
ID
Stim.
Socket
Spring
Contact
Hardware
ID
I 9
60
191
125
K7 7 38
41
K8
34
178
74
K9
33
51
104
L10
56
189
2
L11
59
64
30
L7
11
40
42
L8
31
50
35
L9
36
179
5
M10
46
184
69
M11
49
59
100
M5 8 165
14
M6
18
170
78
M7
21
45
105
M8
28
175
7
M9
39
54
33
N10
43
56
34
N11
50
186
70
N12
53
61
97
N13
63
66
27
N5
14
168
77
N6
17
43
108
N7
24
173
10
N8
27
48
38
N9
40
181
6
O10
44
183
3
O11
47
58
31
O12
54
188
67
O13
57
63
98
O3 3 36
44
O4
10
166
80
O5
13
41
107
O6
20
171
9
O7
23
46
37
O8
30
176
73
O9
37
53
101
P1 2 34
82
P10
41
55
102
P11
48
185
4
P12
51
60
32
P13
58
190
68
P14
61
65
95
P2 1 35
112
P3 6 164
79
P4 9 39
110
P5
16
169
12
P6
19
44
40
P7
26
174
76
P8
29
49
103
P9
38
180
71
R10
42
182
72
R11
45
57
99
R12
52
187
1
R13
55
62
29
R14
62
192
65
R2 4 163
13
R3 5 37
109
R4
12
167
11
R5
15
42
39
R6
22
172
75
R7
25
47
106
R8
32
177
8
R9
35
52
36
GND
64
254
Stimulation Connector Socket 1
Electrode
ID
Stim.
Socket
Spring
Contact
Hardware
ID
A2 2 1
196
B1 4 133
130
B2 1 2
226
C1 5 4
223
C2 6 134
193
C3 3 3
158
D1
12
137
254
D2 9 6
224
D3
10
136
194
E1
15 9 55
E2
16
139
25
E3
13 8 123
E4
14
138
93
E5 8 135
253
F1
22
142
91
F2
19
11
56
F3
20
141
26
F4
17
10
124
F5
18
140
94
G1
25
14
122
G2
26
144
92
G3
23
13
53
G4
24
143
23
G5
21
12
121
G6
11 7 156
G7 7 5
155
H1
32
147
21
H2
29
16
119
H3
30
146
89
H4
27
15
54
H5
28
145
24
H6
31
17
51
H7
34
148
90
I 1
35
19
52
I 2
38
150
87
I 3
37
20
117
I 4
40
151
19
I 5
39
21
49
I 6
36
149
22
I 7
33
18
120
I 8
60
161
16
K1
42
152
88
K2
41
22
118
K3
44
153
20
K4
43
23
50
K5
46
154
85
K6
56
159
15
L1
45
24
115
L2
48
155
17
L3
47
25
47
L4
50
156
86
L5
49
26
116
L6
59
31
46
M1
52
157
18
M2
51
27
48
M3
54
158
83
M4
53
28
113
N1
55
29
45
N2
58
160
84
N3
57
30
114
N4
63
33
43
O1
62
162
81
O2
61
32
111
GND
64
253
Stim. Socket = Stimulation socket number in the connectors 1 to 4
Spring Contact = Spring contacts in the lid of the amplifier
Hardware ID = Hardware channel ID of MC_Rack hardware channels, using the linear layout
Electrode ID = Electrode ID of the MEA electrode in the 16 x 16 layout grid
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2014 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
USB-MEA256-System Manual
6.6 Data Sheet 256ThinMEA
34
256ThinMEA
256ThinMEA200/30iR-ITO
Thin microelectrode array with 16 x 16 layout.
The electrodes are embedded in a very thin
glass substrate on a robust ceramic carrier.
Contact pads and tracks are made from
transparent ITO for high resolution imaging.
Technical Specifications 256ThinMEA200/30iR-ITO
64
63
Connector 4
1
2
63
64
2
1
Connector 1
A B C D E F G H I K L M N O P R
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
A B C D E F G H I K L M N O P R
Connector 3
64
63
1
2
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
Connector 2
64
63
1
2
Temperature compatibility 0 - 125 °C
Dimension (W x D x H) 49 mm x 49 mm x 1 mm
Thickness of the glass part 180 m
Base material Glass on ceramic carrier
Contact pads and track material Indium tin oxide (ITO)
Electrode diameter 30 m
Interelectrode distance (centre to centre) 200 m
Electrode height Planar
Electrode type Titanium nitride (TiN)
Isolation type Silicon nitride (SiN)
Electrode impedance 30 - 50 k
Electrode layout grid 16 x 16
Number of recording electrodes 252
Number of reference electrodes 4 internal reference electrodes (iR)
Contact pads for reference electrodes (connected to ground) 4
MC_Rack
Source layout in “Data Source Setup” Configuration
Channel map 16 x 16.cmp
MEA perfusion chamber (w/o) Without ring
(gr) Glass ring: ID +/- 19 mm, OD 24 mm, height 6 / 12 mm
(pr) Plastic ring without thread: ID 26.5 mm, OD 30 mm, height 6 / 3 mm
(pr-T) Plastic ring with thread: ID 26 mm, OD 30 mm, height 6 / 15 mm
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2013 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
256ThinMEA
A 2 B 1 C 2 E 5 D 3 D 1 E 4 E 2 F 5 F 3 F 1 G 4 G 2 H 5 H 3 H 1 H 7 I 6 I 2 I 4 K 1 K 3 K 5 L 2 L 4 M 1 M 3 K 6 N 2 I 8 O 1 GND
B 2 C 3 C 1 G 7 D 2 G 6 E 3 E 1 F 4 F 2 G 5 G 3 G 1 H 4 H 2 H 6 I 7 I 1 I 3 I 5 K 2 K 4 L 1 L 3 L 5 M 2 M 4 N 1 N3 L 6 O 2 N 4
D 4
GND
A 3
B 3
H 8
F 6
B 4
C 4
A 4
F 7
C 5
A 5
D 6
B 6
E 7
C 7
A 7
D 8
B 8
F 8
G 9
A 9
C 9
E 9
B10
D10
A11
C11
E11
B12
D12
A13
C13
E12
B14
A15
B16
D 5
B 5
E 6
C 6
A 6
D 7
B 7
E 8
C 8
A 8
G 8
F 9
B 9
D 9
A10
C10
E10
B11
D11
A12
C12
F10
B13
G10
A14
C14
B15
B 1 C 1 D 1 E 1 F 1 G 1 H 1 I 1 K 1 L 1 M 1 N 1 O 1 P 1
A 2 B 2 C 2 D 2 E 2 F 2 G 2 H 2 I 2 K 2 L2 M 2 N 2 O 2 P 2 R 2
A 3 B 3 C 3 D 3 E 3 F 3 G 3 H 3 I 3 K 3 L 3 M 3 N 3 O 3 P 3 R 3
A 4 B 4 C 4 D 4 E 4 F 4 G 4 H 4 I 4 K 4 L 4 M 4 N 4 O 4 P 4 R 4
A 5 B 5 C 5 D 5 E 5 F 5 G 5 H 5 I 5 K 5 L 5 M 5 N 5 O 5 P 5 R 5
A 6 B 6 C 6 D 6 E 6 F 6 G 6 H 6 I 6 K 6 L 6 M 6 N 6 O 6 P 6 R 6
A 7 B 7 C 7 D 7 E 7 F 7 G 7 H 7 I 7 K 7 L 7 M 7 N 7 O 7 P 7 R 7
A 8 B 8 C 8 D 8 E 8 F 8 G 8 H 8 I 8 K 8 L 8 M 8 N 8 O 8 P 8 R 8
A 9 B 9 C 9 D 9 E 9 F 9 G 9 H 9 I 9 K 9 L 9 M 9 N 9 O 9 P 9 R 9
A10 B10 C10 D10 E10 F10 G10 H10 I10 K10 L10 M10 N10 O10 P10 R10
A11 B11 C11 D11 E11 F11 G11 H11 I11 K11 L11 M11 N11 O11 P11 R11
A12 B12 C12 D12 E12 F12 G12 H12 I12 K12 L12 M12 N12 O12 P12 R12
A13 B13 C13 D13 E13 F13 G13 H13 I13 K13 L13 M13 N13 O13 P13 R13
A14 B14 C14 D14 E14 F14 G14 H14 I14 K14 L14 M14 N14 O14 P14 R14
A15 B15 C15 D15 E15 F15 G15 H15 I15 K15 L15 M15 N15 O15 P15 R15
B16 C16 D16 E16 F16 G16 H16 I16 K16 L16 M16 N16 O16 P16
P 2
O 3
R 3
K 7
P 4
L 7
C 5
R 5
N 6
P 6
M 7
C 7
R 7
N 8
P 8
L 8
K 9
R 9
O 9
M 9
P10
N10
R11
O11
M11
P12
N12
R13
O13
L11
P14
N13
P 1
R 2
P 3
M 5
O 4
R 4
N 5
P 5
M 6
C 6
R 6
N 7
P 7
M 8
O 8
R 8
K 8
L 9
P 9
N 9
R10
O10
M10
P11
N11
R11
O12
L10
P13
I 9
R14
GND
D13 C15 F11 D14 D16 E13 E15 F12 F14 F16 G13 G15 H12 H14 H16 H10 I11 I15 I13 K16 K14 K12 L15 L13 M16 M14 K11 N15 K10 O16 O14 P15
GND C16 H 9 D15 G11 E14 E16 F13 F15 G12 G14 G16 H13 H15 H11 I10 I16 I14 I12 K15 K13 L16 L14 L112 M15 M13 N16 N14 M12 O15 P16 R15
The letter digit code is the electrode identifier, and refers to the position of the electrode
in the 16 x 16 layout grid. The layout of the letter digit code for the four connectors of
the USB-MEA256 amplifier is shown.
To correlate the pin layout of the connectors, please see the table on the next page.
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2013 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
Stimulation Connector Socket 3
Electrode
ID
Stim.
Socket
Spring
Contact
Hardware
ID
C15
61
98
239
C16
62
222
209
D13
63
99
171
D14
57
96
184
D15
58
220
213
D16
55
95
181
E13
53
94
182
E14
54
218
243
E15
51
93
179
E16
52
217
147
F11
59
97
183
F12
49
92
180
F13
50
216
145
F14
47
91
177
F15
48
215
191
F16
45
90
178
G11
56
219
245
G12
46
214
192
G13
43
89
175
G14
44
213
189
G15
41
88
176
G16
42
212
190
H10
33
84
150
H11
36
209
185
H12
39
87
153
H13
40
211
187
H14
37
86
152
H15
38
210
188
H16
35
85
154
H9
60
221
215
I 10
34
208
186
I 11
31
83
151
I 12
28
205
59
I 13
27
81
149
I 14
30
206
62
I 15
29
82
148
I 16
32
207
61
K10 7 71
63
K11
11
73
247
K12
21
78
250
K13
24
203
57
K14
23
79
251
K15
26
204
60
K16
25
80
146
L12
18
200
220
L13
17
76
248
L14
20
201
221
L15
19
77
252
L16
22
202
58
M12 8 195
217
M13
14
198
218
M14
13
74
246
M15
16
199
222
M16
15
75
249
N14
10
196
216
N15 9 72
244
N16
12
197
219
O14 3 69
28
O15 6 194
214
O16 5 70
64
P15 1 68
96
P16 4 193
126
R15 2 67
66
GND
64
255
Stimulation Connector Socket 4
Electrode
ID
Stim.
Socket
Spring
Contact
Hardware
ID
A10
25
113
236
A11
22
232
205
A12
15
108
169
A13
12
227
143
A14 5 103
241
A15 4 223
141
A3
62
252
195
A4
55
128
159
A5
52
247
131
A6
45
123
229
A7
42
242
202
A8
35
118
166
A9
32
237
138
B10
26
234
206
B11
19
110
170
B12
16
229
144
B13 9 105
242
B14 6 224
211
B15 1 101
240
B16 2 100
210
B3
61
131
225
B4
58
250
198
B5
51
126
162
B6
48
245
134
B7
41
121
232
B8
38
240
201
B9
29
115
233
C10
23
112
167
C11
20
231
139
C12
13
107
237
C13
10
226
212
C14 3 102
172
C4
57
129
228
C5
54
248
197
C6
47
124
161
C7
44
243
133
C8
37
119
231
C9
30
236
203
D10
24
233
140
D11
17
109
238
D12
14
228
207
D4
63
132
157
D5
53
127
227
D6
50
246
200
D7
43
122
164
D8
40
241
136
D9
27
114
168
E10
21
111
235
E11
18
230
208
E12 8 225
142
E6
49
125
230
E7
46
244
199
E8
39
120
163
E9
28
235
137
F10
11
106
174
F6
59
130
160
F7
56
249
132
F8
36
239
135
F9
31
116
165
G10 7 104
173
G8
33
117
234
G9
34
238
204
H8
60
251
129
GND
64
256
Stimulation Connector Socket 2
Electrode
ID
Stim.
Socket
Spring
Contact
Hardware
ID
I 9
60
191
125
K7 7 38
41
K8
34
178
74
K9
33
51
104
L10
56
189
2
L11
59
64
30
L7
11
40
42
L8
31
50
35
L9
36
179
5
M10
46
184
69
M11
49
59
100
M5 8 165
14
M6
18
170
78
M7
21
45
105
M8
28
175
7
M9
39
54
33
N10
43
56
34
N11
50
186
70
N12
53
61
97
N13
63
66
27
N5
14
168
77
N6
17
43
108
N7
24
173
10
N8
27
48
38
N9
40
181
6
O10
44
183
3
O11
47
58
31
O12
54
188
67
O13
57
63
98
O3 3 36
44
O4
10
166
80
O5
13
41
107
O6
20
171
9
O7
23
46
37
O8
30
176
73
O9
37
53
101
P1 2 34
82
P10
41
55
102
P11
48
185
4
P12
51
60
32
P13
58
190
68
P14
61
65
95
P2 1 35
112
P3 6 164
79
P4 9 39
110
P5
16
169
12
P6
19
44
40
P7
26
174
76
P8
29
49
103
P9
38
180
71
R10
42
182
72
R11
45
57
99
R12
52
187
1
R13
55
62
29
R14
62
192
65
R2 4 163
13
R3 5 37
109
R4
12
167
11
R5
15
42
39
R6
22
172
75
R7
25
47
106
R8
32
177
8
R9
35
52
36
GND
64
254
Stimulation Connector Socket 1
Electrode
ID
Stim.
Socket
Spring
Contact
Hardware
ID
A2 2 1
196
B1 4 133
130
B2 1 2
226
C1 5 4
223
C2 6 134
193
C3 3 3
158
D1
12
137
254
D2 9 6
224
D3
10
136
194
E1
15 9 55
E2
16
139
25
E3
13 8 123
E4
14
138
93
E5 8 135
253
F1
22
142
91
F2
19
11
56
F3
20
141
26
F4
17
10
124
F5
18
140
94
G1
25
14
122
G2
26
144
92
G3
23
13
53
G4
24
143
23
G5
21
12
121
G6
11 7 156
G7 7 5
155
H1
32
147
21
H2
29
16
119
H3
30
146
89
H4
27
15
54
H5
28
145
24
H6
31
17
51
H7
34
148
90
I 1
35
19
52
I 2
38
150
87
I 3
37
20
117
I 4
40
151
19
I 5
39
21
49
I 6
36
149
22
I 7
33
18
120
I 8
60
161
16
K1
42
152
88
K2
41
22
118
K3
44
153
20
K4
43
23
50
K5
46
154
85
K6
56
159
15
L1
45
24
115
L2
48
155
17
L3
47
25
47
L4
50
156
86
L5
49
26
116
L6
59
31
46
M1
52
157
18
M2
51
27
48
M3
54
158
83
M4
53
28
113
N1
55
29
45
N2
58
160
84
N3
57
30
114
N4
63
33
43
O1
62
162
81
O2
61
32
111
GND
64
253
256ThinMEA
Stim. Socket = Stimulation socket number in the connectors 1 to 4
Spring Contact = Spring contacts in the lid of the amplifier
Hardware ID = Hardware channel ID of MC_Rack hardware channels, using the linear layout
Electrode ID = Electrode ID of the MEA electrode in the 16 x 16 layout grid
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2013 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
6.7 Data Sheet 256-9wellMEA
Appendix
35
256-9wellMEA
256-9wellMEA300/30iR-ITO-w/o
256-9wellMEA300/30iR-ITO-mq
9-well Microelectrode Array for use
with USB-MEA256-System.
2
Connector 1
1
64
63
64
63
MCS
A B C
E
D
G
H
F
J
Connector 4
1
2
The MEA is not symmetrical
and has to be inserted into
the amplifier with the writing
MCS on top as shown in the
picture beside.
Technical Specifications 9-Well MEA
Temperature compatibility 0 - 125 °C
Dimension (W x D x H) 49 mm x 49 mm x 1 mm
Base material Glass
63
64
Connector 3
1
2
Connector 2
64
63
1
2
Contact pads and track material Indium tin oxide (ITO)
Electrode diameter 30 m (recording), 50 x 200 m (stimulation)
Interelectrode distance (centre to centre) 300 m (recording), 500 m (stimulation)
Electrode height Planar
Electrode type Titanium nitride (TiN)
Isolation type Silicon nitride (SiN)
Electrode impedance 30 - 50 k
Electrode layout grid 6 x 5 recording + 2 stimulation electrodes in each well
Number of recording electrodes 252 (26 recording electrodes in each well)
Number of reference electrodes 9 (1 internal reference electrode (iR) in each well)
Contact pads for reference electrodes (connected to ground) 4
Source layout in “Data Source Setup” Configuration
Channel map 9-well-256MEA.cmp
MEA perfusion chamber (w/o) Without Macrolon quadrat
(mq) Macrolon quadrat with 9 wells:
ID 6.5 x 6.5 mm of each well,
OD 24 x 24 mm of all wells, height 9 mm,
Volumetric capacity of each well: minimum 250 l.
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2013 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
256-9wellMEA
MCS
1.2 mm
A B C
0.8 mm
Slot to insert
an O-ring for
membrane
covering.
Macrolon Quadrat
9 mm
0.8 mm
1.2 mm
6.5 mm
D
G
E
H
6.5 mm
14.60 mm
23.50 mm
49 mm
F
J
Please insert the 256-9wellMEA in correct
orientation into the amplifier: If you can
not read the “MCS”, use the black reference
electrodes as marker.
9-Well macrolon
quadrat for
256-9wellMEA.
256-9wellMEA with macrolon
quadrat and 9well-CC ring
to use it as a culture chamber.
Please insert a foil between
quadrat and ring.
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2013 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
256-9wellMEA
E31 A34 A24 A33 A13 A12 A21 A31 A42 A52 A43 AS2 A64 A55 A45 B34 B24 BS1 B13 B12 B21 B31 B42 B52 B43 BS2 B64 B55 B45 E42 E52 GND
A35 A25 A14 AS1 A23 A22 A32 A41 A51 A62 A63 A53 A54 A44 B35 B25 B14 B33 B23 B22 B32 B41 B51 B62 B63 B53 B54 B44 E41 E51 E62 E43
E32
GND
E21
E22
E23
E12
D45
E13
D44
D55
D64
D53
D43
D52
D42
D31
D21
D12
D13
D33
D24
D34
G31
G21
G12
GS1
G23
G24
G34
G45
G55
G64
G63
GS2
G52
G42
ES1
D54
DS2
D63
D62
D51
D41
D32
D22
D23
DS1
D14
D25
D35
G32
G22
G33
G13
G14
G25
G35
G44
G54
G43
G53
G62
G51
G41
A21 A31 A41 A51
A
A25 A35 A45 A55
D21 D31 D41 D51
D
D25 D35 D45 D55
G21 G31 G41 G51
G
G25 G35 G45 G55
B21 B31 B41 B51
BC
B25 B35 B45 B55
E21 E31 E41 E51
EF
E25 E35 E45 E55
H21 H31 H41 H51
H
H25 H35 H45 H55
C21 C31 C41 C51
C25 C35 C45 C55
F21 F31 F41 F51
F25 F35 F45 F55
I21 I31 I41 I51
J
I25 I35 I45 I55
C35
C25
C14
C23
C33
C22
C32
C41
C51
C62
C63
C43
C54
C44
F41
F51
F62
FS2
F53
F54
F44
F35
F25
F14
F13
FS1
F22
F32
E63
E64
E55
E44
ES2
C34
C24
CS1
C13
C12
C21
C31
C42
C52
C53
CS2
C64
C55
C45
F42
F52
F43
F63
F64
F55
F45
F34
F24
F33
F23
F12
F21
F31
E53
E54
GND
E33 E14 E25 E35 H32 H22 H23 H13 H14 H25 H35 H44 H54 H53 H43 H62 H51 H41 J32 J22 J23 J13 J14 J25 J35 J44 J54 J53 JS2 J62 J 51 J41
GND E24 E34 H31 H21 H12 HS1 H33 H24 H34 H45 H55 H64 H63 HS2 H52 H42 J31 J21 J12 JS1 J33 J24 J34 J45 J55 J64 IJ63 J43 J52 J42 E45
A21 A31 A41 A51
A12 A22 A32 A42 A52 A62
A
S
A13 A23 A33 A43 A53 A63
1
A14 A24 A34 A44 A54 A64
A
Example Well A : The numbering of MEA electrodes
in the 6 x 5 grid per each well follows the standard
numbering scheme for square grids:
The first digit is the column number and the second
digit is the row number. For example, electrode 23
A
S
is positioned in the second column of the third row.
2
Two square electrodes (S1 and S2) per well
are available for stimulation or recording.
There is one big internal reference electrode in each
A25 A35 A45 A55
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
info@multichannelsystems.com
www.multichannelsystems.com
well. The nine reference electrodes are connected to
four contact pads for grounding them.
+49-7121-9 09 25-11
0
© 2013 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
256-9wellMEA
Tables:
Electrode ID
and number
of stimulation
connector socket
in well A, B, and C.
Electrode ID
and number
of stimulation
connector socket
in well D, E, and F.
Well A Well B Well C
Electrode
Electrode
Stimulation
ID
AS1 Conn. 1 No. 7 BS1 Conn. 1 No. 36 CS1 Conn. 2 No. 8
A12 Conn. 1 No. 12 B12 Conn. 1 No. 40 C12 Conn. 2 No. 12
A13 Conn. 1 No. 10 B13 Conn. 1 No. 38 C13 Conn. 2 No. 10
A14 Conn. 1 No. 5 B14 Conn. 1 No. 33 C14 Conn. 2 No. 5
A21 Conn. 1 No. 14 B21 Conn. 1 No. 42 C21 Conn. 2 No. 14
A22 Conn. 1 No. 11 B22 Conn. 1 No. 39 C22 Conn. 2 No. 11
A23 Conn. 1 No. 9 B23 Conn. 1 No. 37 C23 Conn. 2 No. 7
A24 Conn. 1 No. 6 B24 Conn. 1 No. 34 C24 Conn. 2 No. 6
A25 Conn. 1 No. 3 B25 Conn. 1 No. 31 C25 Conn. 2 No. 3
A31 Conn. 1 No. 16 B31 Conn. 1 No. 44 C31 Conn. 2 No. 16
A32 Conn. 1 No. 13 B32 Conn. 1 No. 41 C32 Conn. 2 No. 13
A33 Conn. 1 No. 8 B33 Conn. 1 No. 35 CS1 Conn. 2 No. 9
A34 Conn. 1 No. 4 B34 Conn. 1 No. 32 C34 Conn. 2 No. 4
A35 Conn. 1 No. 1 B35 Conn. 1 No. 29 C35 Conn. 2 No. 1
A41 Conn. 1 No. 15 B41 Conn. 1 No. 43 C41 Conn. 2 No. 15
A42 Conn. 1 No. 18 B42 Conn. 1 No. 46 C42 Conn. 2 No. 18
A43 Conn. 1 No. 22 B43 Conn. 1 No. 50 C43 Conn. 2 No. 23
A44 Conn. 1 No. 27 B44 Conn. 1 No. 55 C44 Conn. 2 No. 27
A45 Conn. 1 No. 30 B45 Conn. 1 No. 58 C45 Conn. 2 No. 30
A51 Conn. 1 No. 17 B51 Conn. 1 No. 45 C51 Conn. 2 No. 17
A52 Conn. 1 No. 20 B52 Conn. 1 No. 48 C52 Conn. 2 No. 20
A53 Conn. 1 No. 23 B53 Conn. 1 No. 51 C53 Conn. 2 No. 22
A54 Conn. 1 No. 25 B54 Conn. 1 No. 53 C54 Conn. 2 No. 25
A55 Conn. 1 No. 28 B55 Conn. 1 No. 56 C55 Conn. 2 No. 28
A62 Conn. 1 No. 19 B62 Conn. 1 No. 47 C62 Conn. 2 No. 19
A63 Conn. 1 No. 21 B63 Conn. 1 No. 49 C63 Conn. 2 No. 21
A64 Conn. 1 No. 26 B64 Conn. 1 No. 54 C64 Conn. 2 No. 26
AS2 Conn. 1 No. 24 BS2 Conn. 1 No. 52 CS2 Conn. 2 No. 24
DS1 Conn. 4 No. 35 ES1 Conn. 4 No. 2 FS1 Conn. 2 No. 51
D12 Conn. 4 No. 40 E12 Conn. 4 No. 59 F12 Conn. 2 No. 54
D13 Conn. 4 No. 38 E13 Conn. 4 No. 57 F13 Conn. 2 No. 49
D14 Conn. 4 No. 33 E14 Conn. 3 No. 61 F14 Conn. 2 No. 47
D21 Conn. 4 No. 42 E21 Conn. 4 No. 61 F21 Conn. 2 No. 56
D22 Conn. 4 No. 39 E22 Conn. 4 No. 62 F22 Conn. 2 No. 53
D23 Conn. 4 No. 37 E23 Conn. 4 No. 60 F23 Conn. 2 No. 52
D24 Conn. 4 No. 34 E24 Conn. 3 No. 62 F24 Conn. 2 No. 48
D25 Conn. 4 No. 31 E25 Conn. 3 No. 59 F25 Conn. 2 No. 45
D31 Conn. 4 No. 44 E31 Conn. 1 No. 2 F31 Conn. 2 No. 58
D32 Conn. 4 No. 41 E32 Conn. 4 No. 63 F32 Conn. 2 No. 55
D33 Conn. 4 No. 36 E33 Conn. 3 No. 63 F33 Conn. 2 No. 50
D34 Conn. 4 No. 32 E34 Conn. 3 No. 60 F34 Conn. 2 No. 46
D35 Conn. 4 No. 29 E35 Conn. 3 No. 57 F35 Conn. 2 No. 43
D41 Conn. 4 No. 43 E41 Conn. 1 No. 57 F41 Conn. 2 No. 29
D42 Conn. 4 No. 46 E42 Conn. 1 No. 60 F42 Conn. 2 No. 32
D43 Conn. 4 No. 50 E43 Conn. 1 No. 63 F43 Conn. 2 No. 36
D44 Conn. 4 No. 55 E44 Conn. 2 No. 63 F44 Conn. 2 No. 41
D45 Conn. 4 No. 58 E45 Conn. 3 No. 2 F45 Conn. 2 No. 44
D51 Conn. 4 No. 45 E51 Conn. 1 No. 59 F51 Conn. 2 No. 31
D52 Conn. 4 No. 48 E52 Conn. 1 No. 62 F52 Conn. 2 No. 34
D53 Conn. 4 No. 52 E53 Conn. 2 No. 60 F53 Conn. 2 No. 37
D54 Conn. 4 No. 53 E54 Conn. 2 No. 62 F54 Conn. 2 No. 39
D55 Conn. 4 No. 56 E55 Conn. 2 No. 61 F55 Conn. 2 No. 42
D62 Conn. 4 No. 47 E62 Conn. 1 No. 61 F62 Conn. 2 No. 33
D63 Conn. 4 No. 49 E63 Conn. 2 No. 57 F63 Conn. 2 No. 38
D64 Conn. 4 No. 54 E64 Conn. 2 No. 59 F64 Conn. 2 No. 40
DS2 Conn. 4 No. 51 ES2 Conn. 2 No. 2
Socket
Well D Well E Well F
Stimulation
ID
Socket
Electrode
ID
Electrode
ID
Stimulation
Socket
Stimulation
Socket
Electrode
ID
Electrode
ID
FS2 Conn. 2 No. 35
Stimulation
Socket
Stimulation
Socket
Electrode ID
and number
of stimulation
connector socket
in well G, H, and J.
Electrode
ID
GS1 Conn. 4 No. 24 HS1 Conn. 3 No. 52 JS1 Conn. 3 No. 24
G12 Conn. 4 No. 26 H12 Conn. 3 No. 54 J12 Conn. 3 No. 26
G13 Conn. 4 No. 21 H13 Conn. 3 No. 49 J13 Conn. 3 No. 21
G14 Conn. 4 No. 19 H14 Conn. 3 No. 47 J14 Conn. 3 No. 19
G21 Conn. 4 No. 28 H21 Conn. 3 No. 56 J21 Conn. 3 No. 28
G22 Conn. 4 No. 25 H22 Conn. 3 No. 53 J22 Conn. 3 No. 25
G23 Conn. 4 No. 22 H23 Conn. 3 No. 51 J23 Conn. 3 No. 23
G24 Conn. 4 No. 20 H24 Conn. 3 No. 48 J24 Conn. 3 No. 20
G25 Conn. 4 No. 17 H25 Conn. 3 No. 45 J25 Conn. 3 No. 17
G31 Conn. 4 No. 30 H31 Conn. 3 No. 58 J31 Conn. 3 No. 30
G32 Conn. 4 No. 27 H32 Conn. 3 No. 55 J32 Conn. 3 No. 27
G33 Conn. 4 No. 23 H33 Conn. 3 No. 50 J33 Conn. 3 No. 22
G34 Conn. 4 No. 18 H34 Conn. 3 No. 46 J34 Conn. 3 No. 18
G35 Conn. 4 No. 15 H35 Conn. 3 No. 43 J35 Conn. 3 No. 15
G41 Conn. 4 No. 1 H41 Conn. 3 No. 29 J41 Conn. 3 No. 1
G42 Conn. 4 No. 4 H42 Conn. 3 No. 32 J42 Conn. 3 No. 4
G43 Conn. 4 No. 9 H43 Conn. 3 No. 35 J43 Conn. 3 No. 8
G44 Conn. 4 No. 13 H44 Conn. 3 No. 41 J44 Conn. 3 No. 13
G45 Conn. 4 No. 16 H45 Conn. 3 No. 44 J45 Conn. 3 No. 16
G51 Conn. 4 No. 3 H51 Conn. 3 No. 31 J51 Conn. 3 No. 3
G52 Conn. 4 No. 6 H52 Conn. 3 No. 34 J52 Conn. 3 No. 6
G53 Conn. 4 No. 7 H53 Conn. 3 No. 37 J53 Conn. 3 No. 9
G54 Conn. 4 No. 11 H54 Conn. 3 No. 39 J54 Conn. 3 No. 11
G55 Conn. 4 No. 14 H55 Conn. 3 No. 42 J55 Conn. 3 No. 14
G62 Conn. 4 No. 5 H62 Conn. 3 No. 33 J62 Conn. 3 No. 5
G63 Conn. 4 No. 10 H63 Conn. 3 No. 38 J63 Conn. 3 No. 10
G64 Conn. 4 No. 12 H64 Conn. 3 No. 40 J64 Conn. 3 No. 12
GS2 Conn. 4 No. 8
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Well G Well H Well J
Stimulation
Socket
Electrode
ID
HS2 Conn. 3 No. 36 JS2 Conn. 3 No. 7
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
Stimulation
Socket
0
Electrode
ID
© 2013 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
Stimulation
Socket
256-9wellMEA
Connector 1 Connector 2 Connector 3 Connector 4
Stim
Elect
Sprin
Hard
Sock
rode
ID
g
Con.
et
1 A35 2 226 1 C35 35 112 1 J41 68 96 1 G41 101 240
2 E31 1 196 2 ES2 34 82 2 E45 67 66 2 ES1 100 210
3 A25 3 158 3 C25 36 44 3 J51 69 28 3 G51 102 172
4 A34 133 130 4 C34 163 13 4 J42 193 126 4 G42 223 141
5 A14 4 223 5 C14 37 109 5 J62 70 64 5 G62 103 241
6 A24 134 193 6 C24 164 79 6 J52 194 214 6 G52 224 211
7 AS1 5 155 7 C23 38 41 7 JS2 71 63 7 G53 104 173
8 A33 135 253 8 CS1 165 14 8 J43 195 217 8 GS2 225 142
9 A23 6 224 9 C33 39 110 9 J53 72 244 9 G43 105 242
10 A13 136 194 10 C13 166 80 10 J63 196 216 10 G63 226 212
11 A22 7 156 11 C22 40 42 11 J54 73 247 11 G54 106 174
12 A12 137 254 12 C12 167 11 12 J64 197 219 12 G64 227 143
13 A32 8 123 13 C32 41 107 13 J44 74 246 13 G44 107 237
14 A21 138 93 14 C21 168 77 14 J55 198 218 14 G55 228 207
15 A41 9 55 15 C41 42 39 15 J35 75 249 15 G35 108 169
16 A31 139 25 16 C31 169 12 16 J45 199 222 16 G45 229 144
17 A51 10 124 17 C51 43 108 17 J25 76 248 17 G25 109 238
18 A42 140 94 18 C42 170 78 18 J34 200 220 18 G34 230 208
19 A62 11 56 19 C62 44 40 19 J14 77 252 19 G14 110 170
20 A52 141 26 20 C52 171 9 20 J24 201 221 20 G24 231 139
21 A63 12 121 21 C63 45 105 21 J13 78 250 21 G13 111 235
22 A43 142 91 22 C53 172 75 22 J33 202 58 22 G23 232 205
23 A53 13 53 23 C43 46 37 23 J23 79 251 23 G33 112 167
24 AS2 143 23 24 CS2 173 10 24 JS1 203 57 24 GS1 233 140
25 A54 14 122 25 C54 47 106 25 J22 80 146 25 G22 113 236
26 A64 144 92 26 C64 174 76 26 J12 204 60 26 G12 234 206
27 A44 15 54 27 C44 48 38 27 J32 81 149 27 G32 114 168
28 A55 145 24 28 C55 175 7 28 J21 205 59 28 G21 235 137
29 B35 16 119 29 F41 49 103 29 H41 82 148 29 D35 115 233
30 A45 146 89 30 C45 176 73 30 J31 206 62 30 G31 236 203
31 B25 17 51 31 F51 50 35 31 H51 83 151 31 D25 116 165
32 B34 147 21 32 F42 177 8 32 H42 207 61 32 D34 237 138
33 B14 18 120 33 F62 51 104 33 H62 84 150 33 D14 117 234
34 B24 148 90 34 F52 178 74 34 H52 208 186 34 D24 238 204
35 B33 19 52 35 FS2 52 36 35 H43 85 154 35 DS1 118 166
36 BS1 149 22 36 F43 179 5 36 HS2 209 185 36 D33 239 135
37 B23 20 117 37 F53 53 101 37 H53 86 152 37 D23 119 231
38 B13 150 87 38 F63 180 71 38 H63 210 188 38 D13 240 201
39 B22 21 49 39 F54 54 33 39 H54 87 153 39 D22 120 163
40 B12 151 19 40 F64 181 6 40 H64 211 187 40 D12 241 136
41 B32 22 118 41 F44 55 102 41 H44 88 176 41 D32 121 232
42 B21 152 88 42 F55 182 72 42 H55 212 190 42 D21 242 202
43 B41 23 50 43 F35 56 34 43 H35 89 175 43 D41 122 164
44 B31 153 20 44 F45 183 3 44 H45 213 189 44 D31 243 133
45 B51 24 115 45 F25 57 99 45 H25 90 178 45 D51 123 229
46 B42 154 85 46 F34 184 69 46 H34 214 192 46 D42 244 199
47 B62 25 47 47 F14 58 31 47 H14 91 177 47 D62 124 161
48 B52 155 17 48 F24 185 4 48 H24 215 191 48 D52 245 134
49 B63 26 116 49 F13 59 100 49 H13 92 180 49 D63 125 230
50 B43 156 86 50 F33 186 70 50 H33 216 145 50 D43 246 200
51 B53 27 48 51 FS1 60 32 51 H23 93 179 51 DS2 126 162
52 BS2 157 18 52 F23 187 1 52 HS1 217 147 52 D53 247 131
53 B54 28 113 53 F22 61 97 53 H22 94 182 53 D54 127 227
54 B64 158 83 54 F12 188 67 54 H12 218 243 54 D64 248 197
55 B44 29 45 55 F32 62 29 55 H32 95 181 55 D44 128 159
56 B55 159 15 56 F21 189 2 56 H21 219 245 56 D55 249 132
57 E41 30 114 57 E63 63 98 57 E35 96 184 57 E13 129 228
58 B45 160 84 58 F31 190 68 58 H31 220 213 58 D45 250 198
59 E51 31 46 59 E64 64 30 59 E25 97 183 59 E12 130 160
60 E42 161 16 60 E53 191 125 60 E34 221 215 60 E23 251 129
61 E62 32 111 61 E55 65 95 61 E14 98 239 61 E21 131 225
62 E52 162 81 62 E54 192 65 62 E24 222 209 62 E22 252 195
63 E43 33 43 63 E44 66 27 63 E33 99 171 63 E32 132 157
GND GND GND GND
ware
ID
Stim
Sock
et
Elect
rode
ID
Spri
ng
Con.
Hard
war
e ID
Stim
Sock
et
Elec
trod
e ID
Spri
ng
Con.
Hard
war
e ID
Stim
Sock
et
Elect
rode
ID
Spri
ng
Con.
ard
are
ID
256-9wellMEA for use with USB-MEA256-System
Stim. Socket = Stimulation socket number in the connectors 1 to 4.
Spring Contact = Spring contacts in the lid of the amplifier, connecting to the contact pads of the 256-9wellMEA.
Hardware ID = Hardware channel ID of MC_Rack hardware channels, using the linear layout.
Electrode ID = Electrode ID of the MEA electrode in the 6 x 5 + 2 layout grid.
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2013 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
USB-MEA256-System Manual
6.8 Data Sheet 256MEA-STIM-C-Con
36
multichannel
256MEA-STIM-C-Con
Connector for Electrode Stimulation of 256MEAs in USB-MEA256-Systems
Connector for inserting the stimulation cable
CB and / or CR from the STG into the provided
stimulation sockets of the USB-MEA256-System.
systems
*
Stimulation cable CB (black) and
CR (red) with connected adapter.
The stimulation cables have a 0.76 mm
connector on the head for the adapter,
and a 1.0 mm connector on the head
for the stimulus generator.
USB-MEA256-System (top view)
with signal generator 256MEA-SG and stimulation
cable CR (red) for stimulation of a electrode,
and cable CB (black) for grounding the system.
The electrodes of the 256MEA can also be used for electrical stimulation. Around the 256MEA,
there are two rows of connection sockets on each side. There is one socket for each electrode
and four ground sockets. These sockets can be used to connect each electrode to a STG,
for example, a stimulus generator of STG4000 series from Multi Channel Systems MCS GmbH.
The ground sockets can be used to connect other devices, like the stimulator, or the perfusion
to the systems ground. You will find the layout map of the sockets in chapter "Pin Layout"
in the Appendix of the USB-MEA256-System manual.
To make it easier to find the correct socket for each electrode, stickers are included to color code
the electrode sockets in four blocks of 2 x 8 sockets, corresponding to the color code used in the
layout map in chapter "Pin Layout". If you want to do electrical stimulation, please attach the stickers
as shown in the image above. The edge of the sticker has to be aligned with the first pair of sockets.
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2013 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
6.9 Data Sheet 256MEA-9well-STIM-ADPT
Appendix
37
multichannel
systems
256MEA-9well-STIM-ADPT
Adapter for Electrode Stimulation of 9-Well MEAs in USB-MEA256-Systems
Front panel of the adapter
256MEA-9well-STIM-ADPT.
The red connectors are for
stimulation of the electrodes
S1 and / or S2 in each well.
The black connectors are
for grounding the system.
Rear panel of the adapter
*
A21 A31 A41 A51
A12 A22 A32 A42 A52 A62
A
S
A13 A23 A33 A43 A53 A63
1
A14 A24 A34 A44 A54 A64
Pin layout
9-Well MEAs
Example Well A : The numbering of MEA electrodes in the
6 x 5 grid per each well follows the standard numbering scheme
for square grids: The first digit is the column number and the
second digit is the row number. For example, electrode 23 is
positioned in the second column of the third row. Two square
electrodes (S1 and S2) per well are available for stimulation or
recording and additionally one big internal reference electrode.
Please fix the adapter 256MEA-9well-STIM-ADPT on the USB-MEA256 amplifier as shown on the foto.
Connect the red connectors (2 mm) with the provided cables to the STG for stimulation of S1 and S2.
The black connectors (2 mm) are for grounding the system.
A25 A35 A45 A55
A
A
S
2
Stimulation of S1 means, that the S1 electrodes in each of the nine wells are stimulated altogether.
Stimulation of S2 means, that the S2 electrodes in each of the nine wells are stimulated altogether.
It is not possible to stimulate only one of the nine stimulation electrodes S1 or S2, for example.
Warning: The 256MEA-9well-STIM-ADPT may only be used together with the
USB-MEA256-System from Multi Channel Systems MCS GmbH, and only for the
specified purpose. Damage of the device and even injuries can result from improper use.
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2013 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
USB-MEA256-System Manual
6.10 256MEA-Signal Generator
38
256MEA-SG
256MEA Signal Generator for use with USB-MEA256-System
Control button
LED
DIP switch
Battery
Gold contact pads
256MEA Signal Generator is a convenient tool for USB-MEA256-System users. Use the 256MEA-SG
instead of setting up an experiment with biological sample for training, controlling, and troubleshooting
purposes. This reduces the number of animal experiments and saves laboratory equipment.
Switch on : Press control button. Switch off : Press control button longer than two seconds.
Important : To change DIP switch position, please switch off the device!
!
Table : DIP switch positions, number of control button presses, and corresponding signals
Switch 1 Switch 2 Control button Signal
presses n times
OFF OFF MEA-SG ON Sinus 0.005 Hz
1 Sinus 0.01 Hz
2 Sinus 0.03 Hz
3 Sinus 1.25 Hz
4 Sinus 12.5 Hz
ON OFF MEA-SG ON EPSP
1 Population Spike
2 Spikes
OFF ON MEA-SG ON ECG Atrium
1 ECG Ventricle
2 Ventricle FP
ON ON MEA-SG ON ERG with Spikes
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2014 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
256MEA-SG
256MEA Signal Generator
Switch
1
OFF
ON
Switch
2
OFF
press
Button
n times
1
256MEA-SG
ON
Signal Source
Artificial Sine Wave (1.75 mV) 0.005 Hz
Note: sine waves < 1Hz might not be
visible because of the hardware filter
bandwidth.
2
3
Sine Wave
4
5
OFF
1
256MEA-SG
Sine Wave
Sine Wave
Sine Wave
Hippocampal Slice
EPSP
ON
1
Hippocampal Slice
Population Spike
Signal Type
0.01 Hz
0.03 Hz
1.25 Hz
12.5 Hz
2
Hippocampal Neurons
Spikes
OFF
ON
1
256MEA-SG
Heart
ECG Atrium
ON
1
Heart
ECG Ventricle
2
Cardiomyocytes
Ventricle FP
ON
ON
1
256MEA-SG
Retina
ERG with Spike
ON
Multi Channel Systems
MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen
Germany
Fon +49-7121-9 09 25Fax
+49-7121-9 09 25-11
info@multichannelsystems.com
www.multichannelsystems.com
0
© 2014 Multi Channel Systems MCS GmbH
Product information is subject to change
without notice.
6.11 Digital IN / OUT Extension
Appendix
16 bit Digital IN / OUT Connector (68-Pin MCS Standard Connector)
Pin 1 GNDP (power ground)
Pin 2 GNDS (signal ground)
Pin 3 - 10 Digital Output channels bit 0 to 7
Pin 11 - 14 GNDS (signals ground)
Pin 15 - 22 Digital output channels bit 8 to 15
Pin 23 - 26 GNDS (signals ground)
Pin 27 - 34 Digital input channels bit 0 to 7
Pin 35 - 38 GNDS (signals ground)
Pin 39 - 46 Digital output channels bit 8 to 15
Pin 47 - 48 GNDS (signals ground)
Pin 49 - 63 Internal use, please do not connect
Pin 64 - 66 GNDS (signals ground)
Pin 67 Positive supply voltage
Pin 68 Negative supply voltage
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USB-MEA256-System Manual
6.12 Scope of Delivery
1 USB-MEA256-System
1 Data acquisition computer
1 USB 2.0 high speed cable (type A - mini B)
4 Lemo coaxial cable with BNC and Lemo connectors (1 m)
1 Power supply unit with country specific power cord
1 Data acquisition and analysis software MC_Rack (Version 3.6.7 and
higher)
1 Data export software MC_DataTool (Version 2.4.3 and higher)
1 USB-MEA256-System Manual
1 Data Sheet
6.13 Contact Information
Local retailer
Please see the list of official MCS distributors on the MCS web site.
User forum
The Multi Channel Systems User Forum provides the opportunity for you to exchange your
experience or thoughts with other users worldwide.
Mailing list
If you have subscribed to the mailing list you will be automatically informed about new software
releases, upcoming events, and other news on the product line. You can subscribe to the list on
the contact form of the MCS web site.
www.multichannelsystems.com
40
7 Index
41
USB-MEA256-System Manual
42
1
16-bit digital IN / OUT 1, 9
2
256MEA 9, 10, 23, 30
16x16 grid..........................................10
Electrode diameter ...........................10
Electrode impedance ........................10
Glass carrier .......................................10
Ground electrodes ............................10
ITO......................................................10
Pin Layout....................................10, 30
Recording electrodes ........................10
SiN isolator ........................................10
Spacing between electrodes ............10
Temperature range...........................10
Tin electrodes ....................................10
256MEA-9well-STIM-ADPT 34
256MEA-STIM-C-Con 33
25MEA Signal Generator 12, 34
9
9 Wells 10, 34
256-9wellMEA 10, 12, 34
256ThinMEA 10, 35
A
A / D converter 1, 9
Amplifier 1, 9, 11
Filter.....................................................9
Signal Amplifikation...........................9
Analog Input A1 to A4 11
Analog input signals 9
AUDIO 11
C
Cardiac signals 9
D
D0 IN 11
D0 OUT 11
Data acquisition 1, 9, 11
DIGITAL IN / OUT 11
Digital IN / OUT Extension 20
E
Electrode Stimulation 20
F
Faraday cage 9
Field potential 9
Filter amplifier 9
bandwidth ...........................................9
frequency band ...................................9
gain ......................................................9
First functional test 12
G
General Performance / Noise Level 12
Ground 11
Grounding 12
Grounding the bath 12
H
Hub 11
L
Lemo connector 11
M
Macrolon Quadrate 10, 34
MC_Rack software 5, 11
MEA Cleaning 10
Microscope 1, 9, 12
N
Noise Level 12
P
Pin Layout 30
Power In 11
POWER LED 11
R
Rear Panel 20
S
Sampling rate 1, 9, 12, 27
Service and Maintenance 12
Stimulation 20
T
TCX 9
Technical Specifications 27
Technical Support 27
Temperature Control 1, 12
Test model probe 12, 30
Toggle Switch I / O 11
Triggering other divices 1, 9
Troubleshooting 23, 27
MEA is defective ............................... 23
Missing Spikes ................................... 23
No Computer Connection ................23
Noise on Single Electrodes............... 23
Triggering does not work ................23
TTL 1, 12, 27
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USB-MEA256-System Manual
U
USB 1, 11, 27
USB hub 11
USB-MEA256-System 1, 11
Using Electrodes for Stimulation 12
Pin Layout .........................................12
Sticker................................................ 12
44
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