Multichannel Systems Cardio2D User Manual

Cardio2D Manual

Information in this document is subject to change without notice.

No part of this document may be reproduced or transmitted without the express written
permission of Multi Channel Systems MCS GmbH.

While every precaution has been taken in the preparation of this document, the publisher
and the author assume no responsibility for errors or omissions, or for damages resulting from
the use of information contained in this document or from the use of programs and source code
that may accompany it. In no event shall the publisher and the author be liable for any loss of
profit or any other commercial damage caused or alleged to have been caused directly or
indirectly by this document.

© 2014 Multi Channel Systems MCS GmbH. All rights reserved.

Printed: 14. 02. 2014

Multi Channel Systems

MCS GmbH

Aspenhaustraße 21

72770 Reutlingen

Germany

Fon [phone] - 0

Fax [phone]

[e-mail]

www.multichannelsystems.com

Microsoft and Windows are registered trademarks of Microsoft Corporation. Products that are
referred to in this document may be either trademarks and/or registered trademarks of their
respective holders and should be noted as such. The publisher and the author make no claim
to these trademark.

Table of Contents

Introduction 1

About this Manual 1
Welcome to Cardio2D and Cardio2D+ 1

Important Information and Instructions 3

Important Safety Advice 3
Guarantee and Liability 4
Operator's Obligations 4
Terms of Use for the Program 5
Limitation of Liability 5

Software and Hardware Installation 7

Installing the Cardio2D Software 7
First Use of Cardio2D after Installation 9
Data Acquisition Hardware 10

Cardio 2D Software 17

The Cardio2D Program : Overview 17
Control Window 25
Control View 26
Viability 27
Labbook 27
Compound / Basic 29
Stimulation of MEA Electrodes 33
Stimulation with MEA2100-System 37
Analyzer Settings 47
Start / Stop 50
Log File 50
Summary Window 51
MEA Window 52
MEA Electrodes 53
Single Channel View 54
Latency / Velocity Window 56
Color Map 58
General Software Features 62

Experimental Proceeding 65

General Performance / Noise Level 65
Detection Parameter 66
Automated Waveform Detection 67

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Cardio2D Manual

Cardio 2D+ Analyzer Software 69

The Cardio2D+ Analyzer Program: Overview 69
Control Window Cardio2D+ 82
MEA Window Cardio2D+ 84
Field Potential Cardio2D+ 85
ROI Selection 93
Heartbeat Cardio2D+ 95
HB Averager 103
Analysis of Field Potential Duration 106

Troubleshooting 109

Troubleshooting 109
Error Messages 109

Theoretical Background 111

Cardiac Action Potential 111

Appendix 113

Technical Support 113
Test Model Probe 113
Contact Information 114

Index 115

iv

1 Introduction

1.1 About this Manual

This manual comprises all important information about the first installation of the hardware
and software, and about the daily work with the instrument. It is assumed that you have already
a basic understanding of technical and software terms. No special skills are required to read this
manual.

If you are using the device for the first time, please read the important safety advice before
installing the hardware and software, where you will find important information about the
installation and first steps.

The printed manual and help are basically the same, so it is up to you which one you will use.
The help offers you the advantage of scrolling through the text in a non-linear fashion, picking
up all information you need, especially if you use the index, and the search function. If you are
going to read larger text passages, however, you may prefer the printed manual.

The device and the software are part of an ongoing developmental process. Please understand
that the provided documentation is not always up to date. The latest information can be found
in the help. Check also the MCS web site (www.multichannelsystems.com) for downloading up-to­date manuals and help files.

1.2 Welcome to Cardio2D and Cardio2D+

The Cardio2D software consists of two main parts: The recording part is called Cardio2D program.
It is for controlling the amplifier, the stimulator, and for scheduling and running the experiments.

The offline analyzer part is called Cardio2D+. The Cardio2D+ analyzer program is intended for
reviewing and graphical representation of recorded experiments and for preparing the graphical
results as far as possible for a publication.

Raw data is recorded in 24 bit resolution when using the MEA2100-System. The other data
acquisition systems record raw data with 16 bit resolution.

1

2 Important Information and Instructions

2.1 Important Safety Advice

Warning: Make sure to read the following advice prior to install or to use the device and the
software. If you do not fulfill all requirements stated below, this may lead to malfunctions or
breakage of connected hardware, or even fatal injuries.

Warning: Obey always the rules of local regulations and laws. Only qualified personnel should
be allowed to perform laboratory work. Work according to good laboratory practice to obtain
best results and to minimize risks.

The product has been built to the state of the art and in accordance with recognized safety
engineering rules. The device may only

 be used for its intended purpose;

 be used when in a perfect condition.

 Improper use could lead to serious, even fatal injuries to the user or third parties and damage

to the device itself or other material damage.

Warning: The device and the software are not intended for medical uses and must not be used
on humans.

Malfunctions which could impair safety should be rectified immediately.

High Voltage

Electrical cords must be properly laid and installed. The length and quality of the cords must be
in accordance with local provisions.

Only qualified technicians may work on the electrical system. It is essential that the accident
prevention regulations and those of the employers' liability associations are observed.

 Each time before starting up, make sure that the mains supply agrees with the specifications

of the product.

 Check the power cord for damage each time the site is changed. Damaged power cords should

be replaced immediately and may never be reused.

 Check the leads for damage. Damaged leads should be replaced immediately and may never be

reused.

 Do not try to insert anything sharp or metallic into the vents or the case.

 Liquids may cause short circuits or other damage. Keep the device and the power cords always

dry. Do not handle it with wet hands.

Requirements for the installation

Make sure that the device is not exposed to direct sunlight. Do not place anything on top of
the device, and do not place it on top of another heat producing device. Never cover the vents,
not even partially, so that the air can circulate freely. Otherwise, the device may overheat.

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Cardio2D Manual

2.2 Guarantee and Liability

The General conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply.
The operator will receive these no later than on conclusion of the contract.

Multi Channel Systems MCS GmbH makes no guarantee as to the accuracy of any and all tests
and data generated by the use of the device or the software. It is up to the user to use good
laboratory practice to establish the validity of his findings.

Guarantee and liability claims in the event of injury or material damage are excluded when
they are the result of one of the following.

 Improper use of the device.

 Improper installation, commissioning, operation or maintenance of the device.

 Operating the device when the safety and protective devices are defective and/or inoperable.

 Non-observance of the instructions in the manual with regard to transport, storage, installation,

commissioning, operation or maintenance of the device.

 Unauthorized structural alterations to the device.

 Unauthorized modifications to the system settings.

 Inadequate monitoring of device components subject to wear.

 Improperly executed and unauthorized repairs.

 Unauthorized opening of the device or its components.

 Catastrophic events due to the effect of foreign bodies or acts of God.

2.3 Operator's Obligations

The operator is obliged to allow only persons to work on the device, who

 are familiar with the safety at work and accident prevention regulations and have been

instructed how to use the device;

 are professionally qualified or have specialist knowledge and training and have received

instruction in the use of the device;

 have read and understood the chapter on safety and the warning instructions in this manual

and confirmed this with their signature.

It must be monitored at regular intervals that the operating personnel are working safely.

Personnel still undergoing training may only work on the device under the supervision of
an experienced person.

4

2.4 Terms of Use for the Program

You are free to use the program for its intended purpose. You agree that you will not decompile,
reverse engineer, or otherwise attempt to discover the source code of the software.

2.5 Limitation of Liability

Multi Channel Systems MCS GmbH makes no guaranty as to the accuracy of any and all tests and
data generated by the use the software. It is up to the user to use good laboratory practice to
establish the validity of his findings.

To the maximum extent permitted by applicable law, in no event shall Multi Channel Systems
MCS GmbH or its suppliers be liable for any special, incidental, indirect, or consequential damages
whatsoever (including, without limitation, injuries, damages for data loss, loss of business profits,
business interruption, loss of business information, or any other pecuniary loss) arising out of the
use of or inability to use the program or the provision of or failure to provide Support Services,
even if Multi Channel Systems MCS GmbH has been advised of the possibility of such damages.

Important Information and Instructions

5

3 Software and Hardware Installation

3.1 Installing the Cardio2D Software

System Requirements

Software: One of the following Microsoft Windows ® operating systems is required:

XP, Vista, Windows 8.1 or Windows 7 (English and German versions supported) with
the NT file system. Other language versions may lead to software errors.

Hardware: Please read chapter "Hardware Acquisition Systems". One of the listed data
acquisition hardware system (MEA2100-System, USB-ME-Systems, USB-MEA256-System,
USB-ME32-FAI-Systems or MC_Card) is recommended by MCS. Due to the amount of recorded
data, a computer with low performance may lead to performance problems; therefore,
Multi Channel Systems recommends an up-to-date computer. Please contact MCS or your local
retailer for more information on recommended computer hardware specification. Please note
that there are sometimes hardware incompatibilities of the data acquisition system and computer
components; or that an inappropriate computer power supply may lead to artefact signals.

Important: You need to have installed the latest data acquisition driver to operate the device,
which is automatically installed with Cardio2D. The installation may be invalid if the data
acquisition does not respond. Please contact Multi Channel Systems MCS GmbH or your local
retailer in this case.

Recommended Operating System Settings

The following automatic services of the Windows operating system interfere with the data
storage on the hard disk and can lead to severe performance limits in Cardio2D. These routines
were designed for use on office computers, but are not very useful for a data acquisition
computer.

 Deselect Windows Indexing Service for all local disks.

 Turn off Optimize hard disk when idle (automatic disk fragmentation).

 It is also not recommended to run any applications in the background when using Cardio2D.

Remove all applications from the Autostart folder.

Important: Please do not run MC_Rack software program from Multi Channel Systems MCS GmbH
in parallel to Cardio2D software!

Please check the system requirements before you install the software. MCS cannot guarantee
that the software works properly if these requirements are not fulfilled.

Important: Please make sure that you have full control over your computer as an administrator.
Otherwise, it is possible that the installed software does not work properly.

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Cardio2D Manual

Installing the Cardio2D Software

Make sure to have a valid license for the Cardio2D software! Please ask
[e-mail].

You do not need a license for the Cardio2D software if you like to test the software for getting
an idea of how it works and what is possible. You can test all functions, run the viability test to
see your data, but you are not able to start an experiment.

Please contact [e-mail] to receive a valid license key.

To enter the following dialog, select "License Key" in menu "Help". Please type the key into
the window "Please enter license key" and confirm the key with "Set".

Double-click the Cardio2D.exe on the installation volume. The installation assistant will show
up and guide you through the installation procedure. Follow the instructions of the installation
assistant.

Note: The hardware driver is installed (or updated) automatically.

Installing the Cardio2D+ Analyzer Software

Double-click the Cardio2D+.exe on the installation volume. The installation assistant will show
up and guide you through the installation procedure. Follow the instructions of the installation
assistant.

You will again be asked for a path to put the log and data files. Cardio2D+ does not produce raw
data files, result files only. Raw data files created by Cardio2D are never changed by Cardio2D+,
but analyzed and saved as result files. It is advisable to use the same subfolders as used by the
Cardio2D.

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Software and Hardware Installation

3.2 First Use of Cardio2D after Installation

Double-click the Cardio2D program icon. The "Data Acquisition Settings" dialog appears.

You have to select the data acquisition device, if more than one data acquisition is available.
Select the "Sample Rate", the "Range" (when using the MC_Card only), and the gain as an
intrinsic property of the connected amplifier. The "Resolution", the number of "Channels" and
the "Driver Version" are displayed. Select the type of MEA or FlexMEA. Please save these entries
with the command "Save Defaults". Otherwise the dialog will appear each time you want to start
Cardio2D. It is possible to change the default settings or to save several default files if you work
with different configuration. The dialog is also available in the "Settings" menu in "Data
Acquisition".

You will be asked for a path to put the log and data files. The program will create subfolders for
each of these file types. It is advisable to put all three subfolders in a common Cardio2D directory.
These settings can be changed later, please see main menu "Settings", "File Location".

In case you have to reinstall the software, you will be asked for the file locations again.

Note: It is recommended to save the settings in the "Data Acquisition Settings" dialog as default.
This way you do not have to type them in again, when restarting Cardio2D.

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Cardio2D Manual

For saving the settings as default, please enter "File" menu "Save Defaults".

3.3 Data Acquisition Hardware

Caution: You have acquired a high performance data acquisition and analysis computer. Do not
modify the system, do not install new hard- or software, or another operating system without
asking MCS or your local retailer for advice. Cardio2D do not install virus scanners or firewalls
because these programs are known to interfere with the data transfer to the hard disk. MCS
cannot guarantee that a modified system is fully operational. Even data loss may occur.

Warning: The operating system settings of the data acquisition computer were
preconfigured by MCS and should not be changed by the user. Changing these
settings can lead to program instabilities and data loss.

Please read chapter "Data Acquisition Settings" in "First Use of Cardio2D after Installation".

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Software and Hardware Installation

Data Acquisition with the MEA2100-Systems

The MEA2100 recording system is an all-in one solution consisting of headstage and interface
board. The MEA2100-System with integrated amplification, data acquisition, online signal
processing, and integrated stimulus generator. You can connect one or two headstages to the
interface board. The MEA2100-System uses an USB 2.0 connector per headstage to transfer
digitized data to any computer. Please read the MEA2100-System manual for detailed
information.

Data Acquisition with the USB-ME-Systems

The external data acquisition board USB-ME256-System is a stand-alone solution for acquiring
data from up to 256 channels from up to four MEAs. It can replace the internal computer
hardware MC_Card. The analog input signals are acquired and digitized by the USB-ME256-System
and the digital electrode signals are transmitted to the connected computer via universal serial
bus (High Speed USB 2.0). Thus, it is possible to use any computer for data processing. Recorded
data is graphed, analyzed, and reviewed with Cardio2D and Cardio2D+ program. The USB-ME256­System supports 256 channels with an amplification factor 1, that means no amplification, data
acquisition only. You can connect, for example, up to four MEA1060 amplifiers via 68-pin MCS
high grade cables.

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Cardio2D Manual

The USB-ME128-System and the USB-ME64-System are in principle the same devices as the
USB-ME256-System, but with 128 or 64 electrode channels respectively.

Data Acquisition with the MC_Card

The data acquisition computer with the with the internal data acquisition board MC_Card comes
preinstalled and preconfigured by MCS for a flawless operation. You should contact your local
retailer for assistance if you want to install additional hard- or software, or if you want to replace
the computer, as incompatibilities of hardware components or software settings with the
MC_Card or Cardio2D may occur.

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Software and Hardware Installation

Data Acquisition with the USB-MEA256-Systems

The USB-MEA256-System is a compact and portable stand-alone solution for MEA recordings with
integrated amplification, data acquisition, and analog / digital conversion. The system acquires
data from up to 252 electrode channels, four additional analog channels, and 16 digital IN / OUT
channels. The digitized electrode data is transmitted to the connected computer via universal
serial bus (High Speed USB 2.0). Thus, it is possible to use any computer as a data acquisition
computer, also a laptop. Recorded data is graphed, analyzed, and reviewed with Cardio2D and
Cardio2D+ program. The integrated filter amplifier supports 252 electrode channels. The gain
of 1100 is fixed. The bandwidth of 1 Hz to 3 kHz is suitable for a broad range of applications.

Data Acquisition with the USB-ME32-FAI-Systems

The USB-ME32-FAI-System is a very compact and portable stand-alone solution. It features an
integrated 32-channel filter amplifier and data acquisition. The digitally converted electrode
signals are transmitted to the connected computer via universal serial bus (High Speed USB 2.0).
As in other USB-ME-Systems, it is possible to use any computer as a data acquisition computer,
also a laptop. The size of the complete system is small enough to fit into a laptop bag together
with the computer. The system is for use with a 32-channel miniature amplifier MPA32I. You can
directly connect the EcoFlexMEA36 to the 32-channel miniature preamplifier for recording with
Cardio2D.

Overview: Possible Configuration of Data Acquisition System and Microelectrode Array
MEA

MEA2100:

MEA2100-HS120:

 MEA_12x12_200_30

MEA2100-HS2x60:

 MEA_6Well_60MEA_200_30

 MEA_8x8_100_10

 MEA_8x8_200_30

 MEA_8x8_1000_30

 MEA_6x10_500_10

 MEA_6x10_500_30

MEA2100-HS60:

 MEA_6Well_60MEA_200_30

 MEA_8x8_100_10

 MEA_8x8_200_30

 MEA_8x8_1000_30

 MEA_6x10_500_10

 MEA_6x10_500_30

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Cardio2D Manual
MC_Card 64:

 MEA_6Well_60MEA_200_30

 MEA_8x8_100_10

 MEA_8x8_200_30

 MEA_8x8_1000_30

 MEA_6x10_500_10

 MEA_6x10_500_30

 FlexMEA72_750_100

 FlexMEA_6x6_300_30 Note: It is possible to connect one or two FlexMEAs!

 EcoFlexMEA_6x6_300_50 Note: It is possible to connect one or two EcoFlexMEAs!

MC_Card 128:

 MEA_6Well_60MEA_200_30

 MEA_8x8_100_10

 MEA_8x8_200_30

 MEA_8x8_1000_30

 MEA_6x10_500_10

 MEA_6x10_500_30

 FlexMEA72_750_100

 FlexMEA_6x6_300_30 Note: It is possible to connect one or two FlexMEAs

per 68-pin MCS standard connector!

 EcoFlexMEA_6x6_300_50 Note: It is possible to connect one or two EcoFlexMEAs

per 68-pin MCS standard connector!

14

USB-MEA64:

 MEA_6Well_60MEA_200_30

 MEA_8x8_100_10

 MEA_8x8_200_30

 MEA_8x8_1000_30

 MEA_6x10_500_10

 MEA_6x10_500_30

USB-MEA256:

 MEA_16x16_100_30

 MEA_9Well_256MEA_300_30

USB-ME32

Software and Hardware Installation

 EcoFlexMEA_6x6_300_50

 FlexMEA_6x6_300_30

USB-ME64:

 MEA_6Well_60MEA_200_30

 MEA_8x8_100_10

 MEA_8x8_200_30

 MEA_8x8_1000_30

 MEA_6x10_500_10

 MEA_6x10_500_30

 FlexMEA72_750_100

 FlexMEA_6x6_300_30 Note: It is possible to connect one or two FlexMEAs!

 EcoFlexMEA_6x6_300_50 Note: It is possible to connect one or two EcoFlexMEAs!

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Cardio2D Manual
USB-ME128:

 MEA_6Well_60MEA_200_30

 MEA_8x8_100_10

 MEA_8x8_200_30

 MEA_8x8_1000_30

 MEA_6x10_500_10

 MEA_6x10_500_30

 FlexMEA72_750_100

 FlexMEA_6x6_300_30 Note: It is possible to connect one or two FlexMEAs

per 68-pin MCS standard connector!

 EcoFlexMEA_6x6_300_50 Note: It is possible to connect one or two EcoFlexMEAs

per 68-pin MCS standard connector!

USB-ME256:

 MEA_6Well_60MEA_200_30

 MEA_8x8_100_10

 MEA_8x8_200_30

 MEA_8x8_1000_30

 MEA_6x10_500_10

 MEA_6x10_500_30

 FlexMEA72_750_10

 FlexMEA_6x6_300_30 Note: It is possible to connect one or two FlexMEAs

per 68-pin MCS standard connector!

 EcoFlexMEA_6x6_300_50 Note: It is possible to connect one or two EcoFlexMEAs

per 68-pin MCS standard connector!

16

4 Cardio 2D Software

4.1 The Cardio2D Program : Overview

Main Window: Overview

The main window of Cardio2D consists of two in case of several connected MEAs of three window
panes. The left "Control Window" is for controlling and documentation of the experiment.
The bigger window on the right side, the "MEA Window" displays heartbeats in different
characteristics in real-time monitoring. The "Summary" window is only available, if more than
one MEA is connected.

You have one single "Control Window" only, but depending on the connected hardware you can
have up to nine different MEA tabbed pages in the "MEA Window" which correspond with the
"Summary" window respectively. If you connect, for example, an USB-MEA256-System for data
acquisition, you can use it together with a 256-9wellMEA and each of the nine wells is displayed
and controlled separate in nine MEA window tabs, MEA A to MEA I.

If you connect, for example, an USB-ME256-System for data acquisition, it is possible to connect up
to four MEAs, MEA_1 to MEA_4. Each MEA window tab will display the heartbeats with different
settings in the four views of the "MEA Tabbed Pages" in the "MEA Window" independent from
each other. Via the "Control Window" you start and stop the experimental protocol
simultaneously.

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Cardio2D Manual

Control Window

The experimental protocol is visualized by three icons on the left orange control view.
The icons can be clicked for editing the protocol. Below the control view the log file
documents the ongoing experimental proceedings.

Summary Window

The "Summary" window is only available, if more than one MEA is connected. It presents
an overview about the layout of the MEAs which are connected and you can select or deselect
the recording of connected MEAs. The summary corresponds with the MEA tabbed pages in the
"MEA Window".

MEA Window

The "MEA Window" is divided into four single views. All of them display heartbeats in different
characteristics. If more than one MEA is connected to the experimental setup, each MEA can be
operated separately via the "MEA Tabbed Pages". Depending on the hardware up to nine MEAs
(for nine wells of the 256-9wellMEA) are available. Select the respective MEA in the "Summary"
window to display the data traces in the MEA window. If a MEA is disabled via check box in the
"Summary" window, the corresponding MEA tabbed page will be removed from the display,
and no data is recorded.

Five different types of MEAs are implemented: The standard MEA with an 8 x 8 electrode layout
grid (the diameter of the MEA electrodes and the spacing between them differs), a MEA with a
6x10 electrode layout grid, the 256MEA with a 16 x 16 layout grid, the 256-9wellMEA with nine
separate wells (shown in the picture above), or the EcoFlexMEA36 with 6 x 6 electrode layout grid.
The design of the MEA window is automatically adjusted to the type of the MEA, please see
chapter "Data Acquisition Settings" in First Use of Cardio2D after Installation.

Recorded data traces are displayed in the MEA electrodes pane. You can zoom to any channel
by clicking on a channel in the MEA electrodes plate. The zoomed channel is displayed in the
larger single channel view. The single channel view has three tabs, "FP" field potential, "HB"
heartbeat and "Ave" average. The heartbeat window shows either a diagram of the RR interval
or of the frequency of the heartbeats, the heart rate. The current values of the RRI or the
frequency are displayed above in red numbers.

The color map displays the propagation of a heartbeat from the pacemaker to the outer regions
of the cardiac cell culture or tissue. The isochrones are indicated by black lines. The colors indicate
the latency distribution over the MEA. Red color represents the pacemaker when using the color
map "jet inverse".

The latency / velocity window shows the latency of the heartbeat in units of μs or the velocity
in units of mm per ms. Both parameters are measured between the first and the last detected
electrode in the range. The current absolute values are displayed in red letters above the plot.

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Cardio 2D Software

Main Menu

File

Menu to open or save templates, to restore and save defaults, and to exit the program.

If you design experiments for a certain purpose, and you want to reuse the experimental schedule,
please save the file as a template. You can reuse the template file as often as necessary. You can
save as much templates as needed. You can also save local and global settings as a template.
The inscriptions appear as soon as you open the template and there is no need to fill in again.

Factory set defaults are the setting parameters, which are used when starting Cardio2D for the
first time. Please enter your own defaults, for example, in "Data Acquisition Settings" dialog after
installing the software. Click "Save Defaults" to save your settings as a default. This way your
setting parameters will be present, each time you start Cardio2D. To restore default settings,
please select "Restore Defaults".

Experiment

Menu to schedule an experiment. Selecting "Basic" or "Compound" changes the icons in the
"Control Window". "Basic" defines the default settings for an usual experiment, "Compound"
gives the possibility to apply an user defined series of compounds.

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Cardio2D Manual

Tools

Menu to activate the offset correction, if necessary. Enable the option "Offset Correction" and the
following dialog appears.

An offset correction may be necessary, if you observe disturbing voltage offset on the input
channels. Click the check box "Correct Offset" and Cardio2D takes 100 ms of the recorded data in
the moment when the check box is selected to calculate the DC offset. The mean of this 100 ms
sweep is subtracted from the recorded data as long as the "Correct Offset" check box is activated.
The offset correction works separately for each available channel. Click the "Update" button to
update the calculation of the offset correction. This option is available when Cardio2D is running.

Settings

Menu to define the data acquisition device and to determine the path for the data and log files.

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Cardio 2D Software

Click "Data Acquisition" in the Settings menu. The "Data Acquisition Settings" dialog opens. This
dialog is the same which shows up before starting Cardio2D for the first time. Please see above.

Please enter the drop down menus to select the current data acquisition. In dependency of the
data acquisition device you can modify the "Sample 'Rate" and the "Range". The "Resolution",
"Number of Channel", and the "Driver Version" will be displayed. Define the type of MEAs used
in the experiment.

Click "File Location" in the Settings menu. The "Location Settings" dialog opens.

Please define the file path for the raw data and the corresponding setting files, the result and
the log files.

When using an USB-ME256 device for data acquisition it is possible to connect up to four MEA
amplifiers, which can be operated independent from each other: That means you can select the
settings for each MEA separately, but the starting and stopping of the experimental protocol is
synchronous for all connected MEAs.

The design of the MEA tab is different depending on the type of the connected MEA.

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Cardio2D Manual

Design of the MEA window when connecting three MEAs, and using a MEA with a 6 x 10 layout
grid in MEA_3 tab.

Design of the MEA window when using one EcoFlexMEA36 with a 6 x 6 layout grid.

Design of the MEA window when using a 256MEA with a 16 x16 layout grid.

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Cardio 2D Software

Click "View Summary Page" to open the "Summary" window. This window is only available, if
more than one MEA at a time is connected. The window presents an overview and all connected
MEAs are visible. The dark green color code spotlights the MEA which is displayed in the MEA
tabbed page. The layout of the MEA is displayed respectively.

You can uncouple the "Summary" window to move it to a different place on the monitor. Click

the
monitoring the color map via "Start" and "Stop" icon.

"Map Settings" icon to open the "Summary Map Settings" dialog and start or stop

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Cardio2D Manual

In this example the "Summary" page shows 60-6wellMEAs with adapted color map.

Help

Menu for starting the Cardio2D online help, to monitor the dialog "About Cardio2D", and to
enter the license key. For a valid license please read chapter "Installing Cardio2D Software".

Select "About Cardio2D".

The dialog shows basic information about the version of Cardio2D.

Note: Please keep in mind that the information of this dialog is necessary in case of support!

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4.2 Control Window

Cardio 2D Software

The "Control Window" is divided into two window panes.

The "Control View" controls the experimental protocol, and the "Log File" documents
the experimental proceedings.

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Cardio2D Manual

4.3 Control View

In the Control window you can enter the settings for setting up and running an experiment. If
more than one MEA is connected each MEA is represented via a tabbed page in "MEA Window".
In "Control View" it is possible to set different parameters for each MEA and to start and stop all
MEAs simultaneously.

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