MP Biomedicals FastPrep-24, 6004-500 Instruction Manual

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Instruction ManualInstruction Manual

Precautionary Instructions

The precautionary instructions found in this section and throughout
this manual are indicated by specific symbols. Understand these
symbols and their definitions before operating this equipment. The
definition of these symbols are as follows;

NOTE :- Throughout this manual "NOTE" may be found. These Notes
are helpful information to aid in the particular area of function being
described.

Text with a "CAUTION" indicator will explain
possible Safety infractions that could have the
potential to cause minor to moderate injury or
damage to equipment.

Text with a "WARNING" indicator will explain
possible Safety infractions that will potentially
cause serious injury and equipment damage.

• Read, understand and practice the precautionary an
operating instructions. Know the limitations and hazards associated
with using this unit. Observe the precautionary and operational
decals placed on the unit.

• DO NOT operate this unit in an environment where other devices
are being used that intentionally radiate electromagnetic energy in
an unshielded manner.

• DO NOT use sharp objects such as a pencil point or ballpoint pen to
operate the buttons on the control panel as damage may result.

• This unit should be operated in temperatures between 15º C to 35º
C, transported and stored in temperatures between 5º C to 43º C,
with Relative Humidity ranging from 30%-60%.

• DO NOT disassemble, modify, or remodel the unit or accessories.

and instead

• This device should be kept out of the reach of children.

WARNING

=CAUTION

=WARNING

CAUTION

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The FastPrep -24 is CE in compliance with the European

Low Voltage and EMC Directives

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LISTED

(LABORATORY EQUIPMENT)

3RRV

U

L

Model #6004-500

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Table of Contents

Section I Introduction ..........................................................................................5

Section II Installation ...........................................................................................6

2.1 Unpacking............................................................................................... 6

2.2 Inspection............................................................................................... 6

2.3 Set-up..................................................................................................... 7

2.4 Connecting the Power............................................................................. 7

Section III Operation............................................................................................8

3.0 Controls & Functions .............................................................................. 8

3.1 Loading & Securing the Samples.............................................................. 9

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3.2 Preparing the FastPrep -24 for Operation.............................................. 12

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3.3 Programming the FastPrep -24.............................................................. 12

3.4 Save Programs...................................................................................... 13

Section IV Specifications....................................................................................15

Section V Warranty and Liability..........................................................................16

Section VI Appendices......................................................................................18

2.1 APPENDIX 1 Maintenance & Cleaning......................................................... 18

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2.2 APPENDIX 2 An Explanation of FastPrep -24 Instrument Speed Settings......21

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2.3 APPENDIX 3 FastPrep -24 Product Line.......................................................22

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2.4 APPENDIX 4 Accessories and Spare Parts for FastPrep -24......................... 30

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I. INTRODUCTION

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Different parts of FastPrep -24 are shown below: (Fig 1 & 2)

The FastPrep -24 Instrument is a high-speed benchtop homogenizer offering the
ultimate in speed and performance for the lysis of biological samples. Indeed,
simultaneous homogenization of 24 samples in 2ml tubes,12 samples in 15ml
tubes ,48 samples in 2ml tubes or 2 samples in 50ml tubes takes place within
40 seconds.

The FastPrep -24 Instrument uses a unique, optimized motion to disrupt cells
through the multidirectional, simultaneous beating of specialized Lysing Matrix
beads on the sample material.

Developed for difficult and resistant samples, the FastPrep -24 Instrument lyses
thoroughly and quickly any tissues and cells and thus allows easy and reproducible
isolation of stable RNA, active proteins and full-length genomic DNA. Samples and
buffers are added to a Lysing Matrix tube containing specialized Lysing Matrix
beads. The ergonomic design of the instrument ensures an easy loading of the
sample tubes that remains securely sealed during the processing. The
homogenization speed and duration times are digitally controlled. After setting
your speed and time with the touch of a button, just push "run", and in less than a
minute your samples are completely lysed.

Dome

Sample Tubes Holder

Sample Tubes

Right Side Wings

Bowl

Securing Knob (ratchet)

Spoke Plate

Micro Switch
(under Dome)

Dome Clip
(Dome Fastener)

Enclosure

Front Panel

Emergency Switch

Fig. 1 (Front view)

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II INSTALLATION

2.1 Unpacking

Carefully remove the FastPrep -24 Instrument and accessories from the shipping
carton.

The list of items included with quantity are given below.

1. AC Power Cord - 1

2. Springs - 8

3. Fuse 10 Amp - 2

4. Screw Driver - 1

5. Instruction Manual - 1

Compare the packing list to the box contents. If there is a discrepancy. call MP
Biomedicals.

CAUTION: Do not lift the device by cover.

2.2 Inspection

Inspect the unit for any damage that may have occurred during shipment.
Should there be any damage, report it to the carrier and contact MP Biomedicals
immediately. Save the packaging material in the event a return is necessary.

2.3 Set-up

To assure safe operation and best result, read this manual before operating the

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FastPrep -24 Instrument. The FastPrep -24 Instrument comes fully assembled,
requiring very little set-up. Install the system on a clean, dry-stable surface
within 4 feet (1.2m) of a compatible electrical outlet.

Ventilation

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Allow 1-2 inches (3-5 cm) of space around he FastPrep -24 Instrument for proper
ventilation. This unit is "FOR INDOOR USE ONLY". Avoid operating in areas of
excessive humidity or extremes of temperature.

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2.4 Connecting the Power

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The FastPrep 24 can work on 110 VAC/60 Hz or 220 VAC/50 Hz. Make sure the
rocker switch located on the rear panel is OFF when connecting the power.
Connect the power cord to the Instrument (power fixture is at the back side) and
plug it in to a compatible outlet.

This symbolizes Alternating Current

III OPERATION

3.0 CONTROLS & FUNCTIONS

Functions of control panel keys
are given below (Ref. Fig. 2)

1. SET Key - Press this key to select
Speed & Time as displayed on
LCD

Speed: Selectable from 4.0 m/s

to 6.5 m/s. In steps of 0.5 m/s
(Default 4.0 m/s).

Tube holder: Selectable

Time: Selectable from I sec. to
60 sec. in steps of I second.
(Default 20 seconds)

2. RUN Key - To Start or Stop the
Instrument.

3. Key - To Increase the selected
value of speed, tube holder &
time.

4. Key - To Decrease the
selected value of speed ,tube
holder & time.

Fig. 2 (Control Panel)

and to choose program

(model 6004)

list of

tube holders

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FastPrep®24

RUN

SPEED

TIME

SET

1

2

3

4

LCD

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3.1 LOADING & SECURING THE SAMPLES

1. Loosen the securing knob (Ratchet Nut) by rotating counter-clockwise until it
come out (Ref. Fig. 3)

2. Remove the securing knob (ratchet Nut).

Fig. 3

3. Take out the assembly of spoke plate and test tube holder (

Fig. 4

Ref Fig. 6)

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Securing Knob
(Ratchet Nut)

Spoke Plate

Three Step

Spoke Plate

Tube Holder

4. Sightly uplift the spoke plate and rotate clockwise so that retention spokes
move away from holes to leave them open for loading (Ref. Fig. 5).

Fig. 5

5. Load sample tubes into the cavities of the sample holder, so that they fit
snugly in the holes. It is preferred to position the tubes symmetrically.

6. When all the sample tubes are loaded, place this assembly into the

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FastPrep -24 Instrument. Align the locking pin of Aluminium three steps with
the holes under the Tube Holder, ensuring its proper placement (Ref. Fig. 6).

Fig. 6

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Retention Spoke Plate

Sample Holder

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Locking Pin

Hole Under the Tube
(for matching)

Aluminium
Three Step

7. Now uplift and rotate the spoke plate counter-clockwise so that the retention
spokes take the position above each sample tube cap. Place the securing
knob (ratchet nut) on this assembly to tighten it completely (Ref. Fig. 7).

Fig. 7

8. Close the dome properly and secure the dome clip.

WARNING: Sample tubes must be secured properly before running the

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FastPrep -24 Instrument.

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Sample Tube

Retention Spoke Plate

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3.2 PREPARING THE FastPrep 24 FOR OPERATION

1. Turn the main Rocker Switch ON (

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2. Factory-set default values automatically program FastPrep -24 to operate at
speed 4.0 m/sec, Tube holder MP:24*2 and run time 20 seconds (to override
these values. See programming)

This symbolizes On and Off supply

3.3 PROGRAMMING THE FastPrep 24

Make sure Emergency Switch is pulled out).

User can take default setting or his own. Five different combination of setting can
be saved as described in next section.

can be changed at this moment. Press p or

q key to change the default Speed to User Selectable Speed. Once speed is selected,

press SET again, flashing of 4.0M/S will stop to indicate that speed has been
selected.

Switch ON Power Supply using Rocker Switch Provided at the Back, Make sure

Emergency Switch is pulled out.

On Power ON the LCD will Show WELCOME followed by MP Bio

Press SET key to start programming

Once SET key is pressed, LCD will show 4.0 M/S which means it's the default
Speed, If Option 1 is needed than press SET key again, the 4.0 M/S message on
LCD will start flashing to indicate that it

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