Motic SMZ168 Instruction Manual

TRD 06: Stereomicroscope User Manual

THE REGIONAL TRAINING WORKSHOP ON LARVAL FISH IDENTIFICATION

AND FISH EARLY LIFE HISTORY SCIENCE

SEAFDEC/TD, SAMUT PRAKAN, THAILAND

16 – 31 MAY 2007

SAFETY INSTRUCTIONS FOR USING THE COMPOUND
MICROSCOPE

In order to fully utilize your microscope safely, please notice the
following important instructions before starting with the microscope.

A. Before turning on the power

Plug the electrical connection of the stand or light source to the
electrical supply. Before plugging and turning on the power,

make sure that the operation voltage of the microscope does
match with the voltage supply.

B. Changing the bulb

1. Before changing the bulb, make sure that the power switch
is off and the power cord has been disconnected from the
main supply.

2. Carefully remove the old light bulb from the socket and plug
the new bulb in.

3. Never touch the glass surface of the bulb with naked hand.
Any grease stain brought onto the bulb by the naked hand will
negatively affect the heat dissipation and, thus, greatly
shorten the life span of the bulb. Clean the bulb surface with
alcohol and tissue if the user has touched the bulb surface
accidentally.

4. Specifications of light bulb can be found on the surface of the
microscope. If required, please contact your local agent for
supply of new light bulbs.

C. Care and cleaning of the microscope

To take good care of the microscope, the user should avoid the
attack of dust and water. If dust and water come to the
microscope, fungus will eventually grow and kill the microscope.
Please notice that once fungus is grown, even after cleaning, the
microscope will have a good chance to be re-infected again.
Moreover, grease stain and fingerprints will distort the image,
which should be avoided.

1. Protection against dust

When the instrument is not used for a long period of time, cover
it with the enclosed dust cover. Never leave the eyepiece tube
open without any covers. Either leave the eyepiece in the tube
(recommended if the microscope is used very frequently) or
cover it with wrapping paper or covering cap. Eyepieces and
other optical accessories, when not in use, are recommended to be
kept in a dry box to protect them against dust and water.

2. Protection against water

The instrument should be kept away from water source, pipeline
or water sink. Humidity in the room where the instrument is
located should be as low as possible (relative humidity should be
kept below 70 %). All the optical accessories are recommended
to be kept in a dry box when not in use. The use of dehumidifier
and 24-hour air conditioning is highly recommended if the
surrounding is very humid.

3. Cleaning

a. If dust is found on the optical surface, try to remove it by

air blower or compressed air.

P.1

P.2

b. For fingerprints, grease stain or dust which cannot be

removed by air blower, two possible methods are
recommended:-

• Breathe lightly on the glass surface and wipe with a piece

of clean cloth, lens paper or cotton swab. Please notice
that small cotton fibre may be left onto the lens surface if
a cotton swab is used.

• Dip a cotton swab or lens paper with a small amount of

absolute alcohol, and clean the lens surface carefully. No

other aggressive solvents should be used.

In no circumstances should the user clean any lens surface
with dry cotton swab, cloth or lens paper. This will scratch
the lens surface causing irreparable damage. Water is not
recommended for cleaning of lens as it will leave some water
stain on the lens surface and if water residue is left on the lens,
fungus can grow causing irreparable damage.

4. Moving the microscope

a. The microscope should not be moved around whenever it

is possible.

b. If moving is unavoidable, the user should ensure that,

before moving, the eyepieces are firmly locked in the
eyepiece tube and all objectives are tightly screwed onto
the nosepiece.

c. The user should move the microscope with both hands,

one hand holding the bottom of the stand and the other
hand holding the upper limb of the microscope. When
moving the microscope, it is always best to have the
eyepiece tube facing" away from the user.

d. The microscope should always be kept vertical when

being moved.

5. Electrical parts of the microscope

a. Before plugging in the power cord with the supply, make

sure that the supplying voltage matches with the operation
voltage of the equipment.

b. Turn off the equipment before plugging in the power card

with the supply.

c. The best practice is to turn the voltage clown to the lowest

before turning off the equipment.

d. Do not turn the power on and off, off and on immediately

as this will shorten the life span of the bulbs and may
cause damage to the electrical system.

e. Users should observe all local safety regulations of the

region. The equipment is manufactured according to CE,
UL and other Safety Instruction. However, users do have
full responsibility to use this equipment safely.

* * SHOULD YOU HAVE ANY PROBLEMS CONCERNING USING
THE MICROSCOPE, PLEASE CONTACT YOUR AGENT
IMMEDIATELY. WE ARE ALWAYS READY TO HELP IN ALL
ASPECTS. * *

* * BECAUSE OF TECHNOLOGICAL ADVANCEMENT, THESE
SAFETY INSTRUCTIONS ARE SUBJECT TO CHANGE WITHOUT
PRIOR NOTICE AND WITHOUT OBLIGATION. * *

YOUR LOCAL AGENT:-

P.3 P.4

SteREO Discovery.V8

Microscopy from Carl Zeiss

A New View of Things

Brilliant Entry into the Class of

Sophisticated Stereomicroscopes

2

SteREO Discovery.V8:

Enhanced Viewing

New high-performance optics – this is the outstanding

performance feature that Carl Zeiss has focused

on with the development of its latest entry level

model in the sophisticated stereomicroscope class.

The SteREO Discovery.V8 impresses with enhanced

resolution, increased contrast and, most notably, a

perceptibly improved stereoscopic impression. As a

result, it offers a image brilliance that is without equal

in this class of instrument. For a visible increase in

information in all biomedical and industrial applica-

tions, the time has come for a new view of things.

SteREO Discovery.V8

at a glance:

• Supreme ease of operation and ergonomic

viewing posture

• Zoom range of 8 : 1

• Optimized optics design for a visible increase

in image information

• Manual focusing drive with adjustable click

stops

• Manual and motorized stands with high stability

• Generous specimen space with high working

distance

• Illumination and contrast methods based on

cold light and LED

PCB
Laterally grazing reflected light
Objective: Plan S 1.0x
Magnification: 16x*

Mouth parts of the common housefly
Oblique illumination in brightfield
transmitted light
Objective: PlanApo S 1.0x
Magnification: 80x*

Wafer structure
Darkfield reflected light
Objective: PlanApo S 1.5x
Magnification: 120x*

* Magnification when looking into the SteREO Discovery.V8

SteREO Discovery.V8

4

stereoscopic impression of the microscopic image.

Even on our SteREO Discovery.V8 entry level model.

Another area we focused on during practical realiza-

tion was the systematic minimization of stray light for

the entire optical system. For exceptionally brilliant

contrast and a new image quality with greater infor-

mation content.

The innovative simultaneous design process during

optical modeling has resulted in a standardized opti-

cal concept for all SteREO microscopes. For signifi-

cantly improved resolution and a perceptibly better

The Optical System: More Than

the Sum of its Individual Components

Today, anyone developing a stereomicroscope that

sets new standards with its optical system has to work

constructively at the very limits of physical feasibility,

taking full advantage of every new possibility offered

by state-of-the-art optical design. With experience and

innovativeness, you don’t have to look any further

than the optical systems from Carl Zeiss.

3 ranges of high-quality objectives
Achromat S: high-contrast images with a pronounced stereoscopic impression
Plan S: flat, distortion-free object fields
PlanApo S: precisely detailed resolution with no color fringes

Parfocally harmonized for needle-sharp
images over the entire magnification
range from 1x to 8x: the new zoom
body of SteREO Discovery.V8

5

a.

b.

c.

The Illumination: Show Your

Specimen in a New Cold Light

Designed for slimline, space-saving light guides, opti-

mized for flicker-free live images on the monitor,

providing constant light output even if the line volt-

age fluctuates, and with ventilation as quiet as a

whisper – the high-intensity CL 1500 ECO cold light

The quality of the illumination – this is all-important

for contrasting in stereomicroscopy. The new fiber-

optic CL 1500 ECO cold light source with its wide

range of light guides and accessories offers you a

variety of opportunities for highlighting your struc-

tures perfectly.

The fiber-optic CL 1500 ECO cold light source
illuminates the specimen precisely with intensive
infrared-free light. Here with a twin-arm goose
neck for oblique reflected light with a targeted
shadow effect.

SMD-Board with white solder resist
Reflected light with different light guides
a. Linear slit light for lateral grazing light
b. Fiber-optic annular slit illuminator for

shadow-free 360° reflected light

c. Annular slit illuminator with polarization

filter device to minimize reflective glare
Objective: Plan S 1.0x
Magnification: 15x

Contrasts with variable optimization in brightfield, darkfield
and oblique light: the transmitted light equipment S

source outperforms conventional fiber-optic systems

thanks to several practical advantages, and offers

excellent performance at a superb price!

Incidentally, lamps and filters can be changed quick-

ly and conveniently. Even when stacked.

6

a.

b.

c.

Or in a Completely Different

Light: With White LEDs

Each of the annular VisiLED illuminators is made up

of eight LED segments that can be switched variably.

A further advantage of noise-free light sources: up

Providing the ability to change rapidly from shadow-

free annular illumination to lateral oblique light, con-

trast optimization through turning of oblique light

around the specimen, continuous rotation of the illumi-

nation for a stereoscopic impression of the object in the

live image – and all this at the push of a button! The list

of new contrasting possibilities offered by the VisiLED

illumination system with its white LEDs is endless!

The VisiLED HCT contrast stage offers a wide range
of contrasts. It contains separate LED illuminators for
brightfield and darkfield, and sliders for finely adjusta­ble oblique light. The LEDs are controlled using
the MC1500 multicontroller.

Mouth parts of the common housefly
Transmitted light with VisiLED HCT contrast stage
a. Brightfield
b. Lateral darkfield
c. Oblique brightfield illumination
Objective: PlanApo S 1.0x
Magnification: 80x

Quiet, durable and offering the best in daylight
quality: VisiLED LED illumination

to 4 illumination settings can be stored and repro-

duced again at any time.

The MC1500 multicontroller of the VisiLED system

allows control of reflected, transmitted and blended

light.

7

1.

2.

3.

4.

1. Interface with digital

image worlds: documentation

SteREO Discovery.V8 creates a connection for a variety of
digital photo and video cameras, via various phototubes, with
interface 60N. For the simple documentation of stereomicro­scopic images, consumer cameras, with their good price/per­formance ratio, are often recommended. Anyone wishing to
satisfy higher demands should use the high-resolution AxioCam
microscope cameras and the AxioVision imaging software
from Carl Zeiss.

2. Brilliant fluorescence: PentaFluar S

PentaFluar S is the name of the retrofittable fluorescence equip­ment for stereomicroscopes belonging to the SteREO Discovery
family. With up to five different filter blocks in the magazine
and special high-performance light sources, this is an outstand­ing addition for contemporary fluorescence applications in
stereomicroscopy.

3. Better in position: the binocular

ergo-phototube S 5-45°

Ergonomics is also about choosing a relaxed sitting position when
operating a microscope. The viewing angle and height have to
coincide. The ergotube allows a free choice of viewing angle
between 5 and 45 degrees. Intermediate tubes and two working
positions for the eyepiece clamps vary the viewing height.

4. Vertical 2D impression:

the objective slide

A must for documentation with subsequent image analysis, e.g.
digital object measurements: the S/doc objective slide for the
SteREO Discovery family of microscopes. Positioned directly
beneath the zoom body, it enables the objective to be shifted
precisely under one of the stereoscopic beam path for a vertical
view of your specimen.

The Expandable Platform:

Flexible For a Variety of Applications

A typical feature of stereomicroscopes is their mod-

ular system design. Equipped with intelligent inter-

faces and fully integrated into the Carl Zeiss systems,

SteREO Discovery.V8, with its comprehensive range

of accessories, offers you a great deal of freedom in

terms of organizing your workplace to suit your own

practical needs.

8

9

System Overview

SteREO Discovery.V8:

The Technical Data

Objectives

Description FWD
Factor (mm)

PlanApo S
0,63x 81

PlanApo S
1,0x 60

PlanApo S
1,5x 30

Plan S
1,0x 81

Achromat S
0,3x 253

Achromat S
0,5x 151

Achromat S
0,63x 115

Achromat S
1,0x 69

Achromat S
1,5x 28

Eyepieces

WPL 10x/23 Br. foc PL 16x/16 Br. foc W 25x/10 foc

Magnification Object Field (mm) Magnification Object Field (mm) Magnification Object Field (mm)

6,3x … 50,4x 36,5 … 4,6 10,1x … 80,6x 25,4 … 3,2 15,8x … 126x 15,9 … 2,0

10x … 80x 23,0 … 2,9 16x … 128x 16,0 … 2,0 25x … 200x 10,0 … 1,3

15x … 120x 15,3 … 1,9 24x … 192x 10,7 … 1,3 37,5x … 300x 6,7 … 0,8

10x … 80x 23,0 … 2,9 16x … 128x 16,0 … 2,0 25x … 200x 10,0 … 1,3

3x … 24x 76,7 … 9,6 4,8x … 38,4x 53,3 … 6,7 7,5x … 60x 33,3 … 4,2

5x … 40x 46,0 … 5,8 8x … 64x 32,0 … 4,0 12,5x … 100x 20,0 … 2,5

6,3x … 50,4x 36,5 … 4,6 10,1x … 80,6x 25,4 … 3,2 15,8x … 126x 15,9 … 2,0

10x … 80x 23,0 … 2,9 16x … 128x 16,0 … 2,0 25x … 200x 10,0 … 1,3

15x … 120x 15,3 … 1,9 24x … 192x 10,7 … 1,3 37,5x … 300x 6,7 … 0,8

Carl Zeiss MicroImaging GmbH

P.O.B. 4041, 37030 Göttingen, Germany
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]

www.zeiss.de/stereo-discovery

46-0087 e 03.2006

Subject to change.

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