Molecular Devices ClonePix 2 Quick Set-up Intructions Manual
07MAN1181.A1
Effective Date: 30-Jan-11
ECO #: 3093
ClonePix
TM
2
QUICK SET-UP INTRUCTIONS MANUAL
SOFTWARE RELEASE 1.2.80.1071
MOLECULAR DEVICES > CLONEPIX 2 QUICK SET-UP INSTRUCTIONS
Contents
What are Quick Set-Up Instructions? .................................................. 3
Starting up ClonePix 2 ........................................................................ 3
Loading Plates .................................................................................... 4
Preparing for a Pick Run ..................................................................... 5
The Pick Run ....................................................................................... 6
Imaging Settings .................................................................................................. 7
Picking Settings .................................................................................................... 8
Sanitize Pin Options .............................................................................................. 9
Start Pick Run ....................................................................................................... 9
Preview ........................................................................................................ 9
Select Wells ................................................................................................. 11
Summary .................................................................................................... 11
Imaging....................................................................................................... 11
Results ........................................................................................................ 12
Picking Review ............................................................................................. 14
Picking ........................................................................................................ 14
Finish .......................................................................................................... 14
Powering Down ClonePix 2 ............................................................... 15
Contact Details ................................................................................. 16
MOLECULAR DEVICES > CLONEPIX 2 QUICK SET-UP INSTRUCTIONS
What are Quick Set-Up
Instructions?
These instructions are designed to enable a new user to undertake a simple picking run on
ClonePix 2. The following instructions offer guidelines only. No attempt should be made to use
ClonePix 2 before the system has been fully installed by an Approved Engineer.
For full information please read the ClonePix 2 Robot Manual and Software Applications
Manual. Please refer to the website for the latest reagents & supplies, replacement parts and
optional extras.
Starting up ClonePix 2
Ensure robot and compressor are plugged in.
Turn on the compressor and ensure compressed air gauge is set to 80 psi (5.5 bar).
Ensure Emergency Stop button is not pressed in.
Switch on ClonePix 2. The HEPA filtration system works all the time that ClonePix 2 is on.
After approximately 2 minutes, initiate ClonePix 2 software.
Wipe out ClonePix 2 bed with 70% ethanol or fresh Sterilizing Agent (K8080) using a lintfree cloth.
Fill the ethanol feed bottle with 70% ethanol and empty the ethanol waste bottle.
Make sure that the correct Picking Pins are installed for the type of cells to be picked.
F1 Picking Pins (400µm internal diameter; X4961) for suspension cell picking from
semi-solid medium.
F2 Picking Pins (700µm internal diameter; X4962) for adherent cell picking from liquid
medium.
Picking Pins should be cleaned by sonication in aQuClean (K2505) and autoclaved. It is
advisable to autoclave the Picking Pin Removal Key (X4948) at the same time. See General
Maintenance section of the Robot Manual for instructions.
If the Picking Pins need to be changed remove the Picking Head and swap the pins. To do
this, click on the Picking Head Management icon, then the Replace Head icon and follow the
on-screen instructions. Refer to the General Maintenance section of the Robot Manual for
guidance on how to remove and replace the head and the pins.
MOLECULAR DEVICES > CLONEPIX 2 QUICK SET-UP INSTRUCTIONS
Loading Plates
It is important to know how to load plates into ClonePix 2 correctly.
A ClonePix 2 bed layout is as shown below:
The Source plate stacker system is located at the rear of the machine, and the Destination
plate stacker system is at the front. Both the Source and Destination stackers have 1
removable cassette (left hand side).
Each cassette can accommodate up to 10 microplates. The plates are fed individually from
the cassette, via the plate holder, onto the bed with automatic lid removal as the plate passes
the plate lid lifter. On completion of colony picking, the de-lidded plate is then returned to the
cassette where the lid is replaced.
Important notes:
Plates loaded into a cassette, via the plate holders, must be of the same type and must
match the plate type selected in the software. Loading of a plate type different from that
selected in the software is likely to damage the plate and the instrument.
Plate holders must be placed level into the cassette and all the way to the back of the
cassette to ensure correct positioning.
When the cassettes are placed in the source and destination stackers, they must be held
firmly in place by the locking bolt on the front left hand side of the stacker systems.
Failure to lock a cassette may cause a malfunction of the collection and/or return of the
microplates.
Source and destination plates must be loaded onto plate holders and then into the
cassettes with lids on and well A1 in the front right-hand corner as shown below:
Imaging station
Wash station
Source Cassette
Destination Cassette
MOLECULAR DEVICES > CLONEPIX 2 QUICK SET-UP INSTRUCTIONS
Orientation of source plates in ClonePix 2
Orientation of destination plates in ClonePix 2
Preparing for a Pick Run
Prior to carrying out a picking run, it is important to make sure that ClonePix 2 is set up
correctly. The Prepare for Pick Run process is designed for this purpose. This process helps
the user to validate that 1) the pins are firing correctly, 2) the camera, pins and microplates
are aligned, and 3) the fluid system is sterile and ready for use.
Click on the Prepare for Pick Run icon and follow the on-screen instructions.
When asked to load a source plate, it is recommended that a blank PetriWell-6 plate is used.
However, the use of any configured plate (those present in the drop-down list) should work.
Once aligned using a configured plate type, ClonePix 2 is then ready for use with any
configured plate type.
The Prepare for Pick Run process does not need to be carried out prior to every picking
run. It must be carried out every time that ClonePix 2 is first powered up.
Click Close Process to return to the Main Navigation Screen.
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