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INSTRUCTION MANUAL
Milwaukee RefractometerMilwaukee Refractometer
Milwaukee Refractometer
Milwaukee RefractometerMilwaukee Refractometer
MA871
Refractometer for SucroseRefractometer for Sucrose
Refractometer for Sucrose
Refractometer for SucroseRefractometer for Sucrose
MeasurementsMeasurements
Measurements
MeasurementsMeasurements
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Instruction Manual MA871 Refractometer for Sucrose Measurements
TABLE OF CONTENTS
FUNCTIONAL DESCRIPTION ............................................................................. 3
GENERAL DESCRIPTION .................................................................................... 5
SPECIFICATIONS .............................................................................................. 6
PRINCIPLE OF OPERATION................................................................................ 7
MEASUREMENT GUIDELINES .............................................................................. 8
CALIBRATION PROCEDURE ............................................................................... 9
MEASUREMENT PROCEDURE ........................................................................... 10
MAKING A STANDARD % BRIX SOLUTION ........................................................ 11
CHANGING TEMPERATURE UNIT ..................................................................... 12
ERROR MESSAGES ........................................................................................... 13
BATTERY REPLACEMENT................................................................................... 14
WARRANTY ..................................................................................................... 15
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FUNCTIONAL DESCRIPTION
DISPLAYDISPLAY
DISPLAY
DISPLAYDISPLAY
A. BATTERY STATUS ICON (BLINKS WHEN LOW BATTERY CONDITION DETECTED)
B. MEASUREMENT IN PROGRESS TAG
C. SETUP: FACTORY CALIBRATION TAG
D. CAL: CALIBRATION TAG
E. PRIMARY DISPLAY (DISPLAYS MEASUREMENT AND ERROR MESSAGES)
F. AUTOMATIC TEMPERATURE COMPENSATION
(BLINKS WHEN TEMPERATURE EXCEEDS 10-40 °C / 50-104 °F RANGE)
G. TEMPERATURE UNITS
H. SECONDARY DISPLAY (DISPLAYS TEMPERATURE MEASUREMENTS; WHEN BLINKING,
TEMPERATURE HAS EXCEEDED OPERATION RANGE: 0-80 °C / 32-176 °F)
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Instruction Manual MA871 Refractometer for Sucrose Measurements
FRONT PANELFRONT PANEL
FRONT PANEL
FRONT PANELFRONT PANEL
A. LIQUID CRYSTAL DISPLAY (LCD)
B. READ KEY (USER MEASUREMENT)
C. ZERO KEY (USER CALIBRATION)
D. ON/OFF
E. PRIMARY DISPLAY
F. SECONDARY DISPLAY
G. STAINLESS STEEL SAMPLE WELL AND PRISM
BOTTOMBOTTOM
BOTTOM
BOTTOMBOTTOM
H. BATTERY COVER
I. BATTERY COMPARTMENT
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GENERAL DESCRIPTION
SIGNIFICANCE OF USE
Thank you for choosing Milwaukee. This instruction manual will provide you the necessary
information for correct use of the meter.
The MA871 is an optical instrument that employs the measurement of refractive index to
determine the % Brix of sugar in aqueous solutions. The method is both simple and quick.
Samples are measured after a simple user calibration with deionized or distilled water.
Within seconds the instrument measures the refractive index of the sample and converts it
to % Brix concentration units. The MA871 digital refractometer eliminates the uncertainity
associated with mechanical refractometers and is easily portable for measurements in the
field.
The measurement technique and temperature compensation employ methodology
recommended in the ICUMSA Methods Book (Internationally recognized body for Sugar
Analysis).
Temperature (in °C or °F) is displayed simultaneously with the measurement on the large
dual level display along with icons for Low Power and other helpful message codes.
Key features include:
• Dual-level LCD
• Automatic Temperature Compensation (ATC)
• Easy setup and storage
• Battery operation with Low Power indicator (BEPS)
• Automatically turns off after 3 minutes of non-use.
Remove the instrument from the packing materials and examine carefully to
ensure no damage has occurred during shipping. If any damage has
occurred, notify your Dealer.
Each MA871 instrument is supplied with:
• 9 V battery
• Instruction manual
Note: Save all packing material until you are sure that the instrument
functions correctly. A defective instrument must be returned in its
original packing.
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Instruction Manual MA871 Refractometer for Sucrose Measurements
SPECIFICATIONS
Range 0 to 85% Brix 0 to 80 °C (32 to 175 °F)
Resolution 0.1% Brix 0.1 °C (0.1 °F)
Accuracy ± 0.2% Brix ± 0.3 °C (± 0.5 °F)
Light Source Yellow LED
Measurement Time Approximately 1.5 seconds
Minimum Sample Volume 100 µL (cover prism totally)
Sample Cell SS ring and flint glass prism
Temperature Compensation Automatic between 10 and 40 °C (50 to 104 °F)
Case Material ABS
Enclosure Rating IP 65
Battery Type/Life 1 x 9 volt AA batteries / 5000 readings
Auto-Shut off After 3 minutes of non-use
Dimensions 19.2 x 10.2 x 6.7 cm (7.5 x 4 x 2.6")
Weight 420 g (14.8 oz.).
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PRINCIPLE OF OPERATION
The Brix determination is made by measuring the refractive index of a solution. Refractive
Index is an optical characteristic of a substance and the number of dissolved particles in
it. Refractive Index is defined as the ratio of the speed of light in empty space to the speed
of light in the substance. A result of this property is that light will “bend”, or change
direction, when it travels through a substance of different refractive index. This is called
refraction.
When passing from a material with a higher to lower refractive index, there is a critical
angle at which an incoming beam of light can no longer refract, but will instead be
reflected off the interface. The critical angle can be used to easily calculate the refractive
index according to the equation:
critical
) = n2 / n
1
sin (θ
Where n2 is the refractive index of the lower-density medium; n1 is the refractive index of
the higher-density medium.
In the MA871, light from an LED passes through a prism in contact with the sample. An
image sensor determines the critical angle at which the light is no longer refracted
through the sample. The MA871 automatically applies temperature compensation to the
measurement and converts the refractive index of the sample to sucrose concentration in
units of percent (by weight) Brix.
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Instruction Manual MA871 Refractometer for Sucrose Measurements
MEASUREMENT GUIDELINES
• Handle instrument carefully. Do not drop.
• Do not immerse instrument under water.
• Do not spray water to any part of instrument except the “sample well” located over
the prism.
• The instrument is intended to measure sugar solutions. Do not expose instrument or
prism to solvents that will damage it. This includes most organic solvents and extremely
hot or cold solutions.
• Particulate matter in a sample may scratch the prism. Absorb sample with a soft
tissue and rinse sample well with deionized or distilled water between samples.
• Use plastic pipettes to transfer all solutions. Do not use metallic tools such as
needles, spoons or tweezers as these will scratch the prism.
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CALIBRATION PROCEDURE
Calibration should be performed daily, before measurements are made, when the
battery has been replaced, or between a long series of measurements.
1. Press the ON/OFF key, then release. Two instrument test screens will be displayed
briefly; an “all segment” screen followed by the percentage of remaining battery life.
When LCD displays dashes, the instrument is ready.
2. Using plastic pipettes, fill the sample well with distilled or deionized water.
Note: If the ZERO sample is subject to intense light such as sunlight or another strong
source, cover the sample well with your hand or other shade during the
calibration.
3. Press the ZERO key. If no error messages appear, your unit is calibrated.
(For a description of ERROR MESSAGES see page 13).
Note: The 0.0 screen will remain until a sample is measured or the power is turned off.
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Instruction Manual MA871 Refractometer for Sucrose Measurements
4. Gently absorb the ZERO water standard with a soft tissue. Use care not to scratch
the prism surface. Wipe off the surface completely. The instrument is ready for
sample measurement.
Note: If instrument is turned off the calibration will not be lost.
MEASUREMENT PROCEDURE
Verify the instrument has been calibrated before taking measurements.
1. Wipe off prism surface located at the bottom of the sample well.
2. Using plastic pipettes, drip sample onto the prism surface. Fill the
well completely.
Note: If the temperature of the sample differs significantly from the
temperature of the instrument, wait approximately 1 minute to
allow thermal equilibration.
3. Press the READ key. Measurement is displayed in units of % BRIX.
Note: The ATC tag blinks and automatic temperature compensation is disabled if the
temperature exceeds the 10-40 °C / 50-104 °F range.
4. Remove sample from the sample well by absorbing with a soft tissue.
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5. Using plastic pipettes, rinse prism and sample well with distilled or deionized water.
Wipe dry. The instrument is ready for the next sample.
MAKING A STANDARD % BRIX SOLUTION
To make a Brix Solution, follow the procedure below:
• Place container (such as a
analytical balance.
• Tare the balance.
• To make an X BRIX solution weigh out X grams of high purity Sucrose (CAS #: 57-50-1)
directly into the container.
• Add distilled or deionized water to the container so the total weight of the solution is
100g.
• Note: Solutions above 60% Brix need to be vigorously stirred or shaken and heated
in a water bath to roughly 40 °C (104 °F). Remove solution from bath when
sucrose has dissolved. Cool completely before use. The total quantity can be
scaled proportionally for smaller containers but accuracy may be sacrificed.
Example with 25% Brix:
% Brix g Sucrose g Water g Total
25 25.000 75.000 100.000
glass vial or dropper bottle that has a cover) on an
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Instruction Manual MA871 Refractometer for Sucrose Measurements
CHANGING TEMPERATURE UNIT
To change the temperature measurement unit from Celsius to Fahrenheit (or vice versa),
follow this procedure.
1. Press and hold the ON/OFF key continuously for aproximately 15 seconds. The
LCD will display the “all segment” screen followed by a screen with the model
number on the primary display and the version number on the secondary display.
Continue pressing the ON/OFF key.
15 seconds
2. While continuing to hold the ON/OFF key, press the ZERO key. The temperature
unit will change from °C to °F or vice versa.
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Instruction Manual MA871 Refractometer for Sucrose Measurements
BATTERY REPLACEMENT
To replace the instrument’s battery, follow these steps:
• Turn the instrument OFF by pressing the ON/OFF key.
• Turn instrument upside down and remove the battery cover by turning it counterclockwise.
• Extract the battery from its location.
• Replace with fresh 9V battery making certain to observe polarity.
• Insert the back battery cover and fasten it by turning clockwise to engage.
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For your Safety don’t use or store the instrument in hazardous environments. To avoid
damages or burns, do not perform any measurement in microwave ovens.
WARRANTY
This instrument is warranted against defects in materials and manufacturing for a period of
2 years from the date of purchase. Electrodes are warranted for 6 months.
If during this period the repair or replacement of parts is required, where the
damage is not due to negligence or erroneous operation by the user, please return
the intrument, electrode and probe to either distributor or our office and the repair
will be effected free of charge.
Damage due to accidents, misuse, tampering or lack of prescribed maintenance is
not covered by the warranty.
Milwaukee/Martini instruments reserves the right to make improvements
in design, construction and appearance of its products without advance
notice.
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Instruction Manual MA871 Refractometer for Sucrose Measurements
THANK YOU FOR CHOOSING
Sales and Technical Service Contacts:
Milwaukee s.r.l.
C.so Leonardo da Vinci 48/50
21013 Gallarate (VA) ITALY
Tel: +39 0331 26 80 09
Fax: +39 0331 26 80 33
e-mail: sales@milwaukee.191.it
Milwaukee Instruments, Inc.
2950 Business Park Drive Rocky Mount, NC
27804 USA
Tel: +1 252 443 3630
Fax: +1 252 443 1937
e-mail: sales@milwaukeetesters.com
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MANMA871 04/09
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