Page 1
Stirred Ultraltration Cells
Models 8003, 8010, 8050, 8200, 8400
User Guide
Page 2
Notice
The information in this document is subject to change
without notice and should not be construed as a
commitment by Millipore Corporation or an afliated
corporation. Neither Millipore Corporation nor any of its
afliated corporations assumes responsibility for any
errors that may appear in this document. This manual
is believed to be complete and accurate at the time of
publication. In no event shall Millipore Corporation or an
afliated corporation be liable for incidental or consequential damages in connection with or arising from the
use of this manual.
© 2008 MILLIPORE CORPORATION. PRINTED IN
THE UNITED STATES OF AMERICA. ALL RIGHTS
RESERVED.
Millipore and Amicon are registered trademarks of Millipore Corporation.
The M mark is a trademark of Millipore Corporation.
Cellosolve is a registered trademark of Union Carbide Chemicals &
Plastics Technology Corporation.
Tygon is a registered trademark of Saint-Gobain Performance Plastics
Corporation.
Saint-Gobain is a registered trademark of Compagnie de Saint-Gobain
Corporation.
99228, Rev. M, 08/08
Page 3
Contents
Introduction ............................................. 1
Models Available ..................................... 1
Safety Features ...................................... 1
Model 8003 Parts List ............................. 2
Model 8010 Parts List ............................. 4
Model 8050 Parts List ............................. 6
Model 8200 Parts List ............................. 8
Model 8400 Parts List ........................... 10
Operating Modes .................................. 12
Unpacking the System ..........................13
Setting Up the System .......................... 14
Operating Guidelines ............................ 17
Limitations ............................................ 18
Cell Operation .......................................19
Shutting Down the System ....................21
Page 4
Sterilization ...........................................21
Storage .................................................22
Maintenance ......................................... 22
Troubleshooting .................................... 23
Specications ....................................... 24
Materials of Construction ...................... 24
Compliance with the Pressure Equipment
Directive, 97/23/EC ...............................25
Product Ordering Information................ 26
Technical Assistance............................. 30
Standard Warranty ................................ 30
Page 5
Introduction
Millipore stirred ultraltration cells are designed for
rapid concentration or purication of macromolecular
solutions in volumes from 3 to 400 mL. All models are
used in conjunction with a stirring table. The table is
magnetically coupled to a stirring bar, which maintains
uid movement during operation, thereby reducing the
negative effects of concentration polarization (i.e., the
buildup of concentrated solutes on the membrane).
Models Available
Model Capacity Millipore Cat. No.
8003 3 mL 5125
8010 10 mL 5121
8050 50 mL 5122
8200 200 mL 5123
8400 400 mL 5124
Safety Features
Cell has a built-in pressure-relief valve, preset to ■
nominal 75 psi (5.3 kg/cm2). The recommended
operating pressure is <55 psi (3.9 kg/cm
Cell remains xed in the retaining stand until ■
the pressure-relief valve is opened and the cap
depressed.
Stand provides a large base for improved cell stability. ■
2
).
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Model 8003 Parts List
A
B
L
K
C
D
E
M
N
F
G
H
I
J
Page 7
Model 8003 Parts List, continued
Item Description Quantity Supplied
A Cap Assembly 1
B Pressure Relief Valve —
C O-Ring 2
D Stirrer Assembly 1
E Body 1
F O-Ring 2
G Membrane —
H Membrane Holder 1
I Elastomeric Tubing 4 in.
J Base 1
K Tube Fitting Assembly 2
L Tubing, Plastic 4 ft
M Stand Assembly 1
N O-Ring 2
3Stirred Ultraltration Cells
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Model 8010 Parts List
A
B
L
K
C
D
E
M
F
G
H
I
J
Page 9
Model 8010 Parts List, continued
Item Description Quantity Supplied
A Cap Assembly 1
B Pressure Relief Valve —
C O-Ring 2
D Stirrer Assembly 1
E Body 1
F O-Ring 2
G Membrane —
H Membrane Holder 1
I Elastomeric Tubing 4 in.
J Base 1
K Tube Fitting Assembly 2
L Tubing, Plastic 4 ft
M Stand Assembly 1
5Stirred Ultraltration Cells
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Model 8050 Parts List
A
B
L
C
D
K
E
M
F
G
H
I
J
Page 11
Model 8050 Parts List, continued
Item Description Quantity Supplied
A Cap Assembly 1
B Pressure Relief Valve —
C O-Ring 2
D Stirrer Assembly 1
E Body 1
F O-Ring 2
G Membrane —
H Membrane Holder 1
I Elastomeric Tubing 4 in.
J Base 1
K Tube Fitting Assembly 2
L Tubing, Plastic 4 ft
M Stand Assembly 1
7Stirred Ultraltration Cells
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Model 8200 Parts List
A
B
K
L
C
D
E
M
F
G
H
I
J
Page 13
Model 8200 Parts List, continued
Item Description Quantity Supplied
A Cap Assembly 1
B Pressure Relief Valve —
C O-Ring 2
D Stirrer Assembly 1
E Body 1
F O-Ring 2
G Membrane —
H Membrane Holder 1
I Elastomeric Tubing 4 in.
J Base 1
K Tube Fitting Assembly 2
L Tubing, Plastic 4 ft
M Stand Assembly 1
9Stirred Ultraltration Cells
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Model 8400 Parts List
A
B
K
C
L
D
E
F
M
G
H
I
J
Page 15
Model 8400 Parts List, continued
Item Description Quantity Supplied
A Cap Assembly 1
B Pressure Relief Valve —
C O-Ring 2
D Stirrer Assembly 1
E Body 1
F O-Ring 2
G Membrane —
H Membrane Holder 1
I Elastomeric Tubing 4 in.
J Base 1
K Tube Fitting Assembly 2
L Tubing, Plastic 4 ft
M Stand Assembly 1
11Stirred Ultraltration Cells
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Operating Modes
Stirred ultraltration cells can be operated in two
modes: concentration and dialtration.
Concentration
In this mode, apply gas pressure directly to the ultraltration cell. Solutes above the membrane molecular
weight cut-off are retained in the cell, while water and
solutes below the cut-off pass into the ltrate and out of
the cell.
Dialtration
Connect the ultraltration cell to an auxiliary reservoir
(Millipore cat. no. 6028) containing a dialtration
solution with the desired microsolute concentration.
The use of stirred cells in the dialtration mode also
requires use of the CDS-10 Concentration/Dialysis
Selector (Milipore cat. no. 6003). The combination of
the auxiliary reservoir and the CDS-10 selector form
a system that keeps the stirred cell uid volume and
macrosolute concentration constant as the ltrate
volume is replaced by the dialtration solution. This
technique provides a simple means for rapid microsolute exchange, and is typically used as a substitute for
dialysis.
Page 17
13Stirred Ultraltration Cells
Unpacking the System
Place cell on a level surface and remove packing
inserts. Turn the pressure relief valve knob to the horizontal position. Hold the body and push the cap down.
Slide the cell out of the retaining stand and remove the
cap assembly with a twisting motion. Pull out the stirrer
assembly and discard packing. Unscrew the base and
remove the membrane holder. Remove the O-ring from
the membrane holder.
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Setting Up the System
To identify the parts of the stirred cell, refer to the
diagrams in the appropriate model parts list section.
This section explains how to install the membrane and
stirring assembly.
Membrane Installation
NOTE: Handle the membrane by its edges, to avoid
scratching or contaminating the surface.
Model 8003 only:1.
Invert the cell body and place the smaller membrane
holder O-ring (Model 8003 Parts List — N) into the
recess. Gently tap to ensure that the O-ring remains
when the body is upright. Place the membrane into
the holder, shiny side up. Then place the larger
membrane holder O-ring (Model 8003 Parts List — F)
down, so that it contacts and seats the membrane
evenly in the bottom of the holder.
All other models (8010, 8050, 8200, and 8400):
Place the membrane into the holder, shiny side up.
Place the O-ring on top of the membrane. Gently
push the O-ring down, so that it contacts and seats
the membrane evenly in the bottom of the holder.
Fit the membrane holder into the cell body, aligning 2.
the tabs on the sides of the holder with the slots in
the base of the cell body.
Invert the cell body and membrane holder; screw the 3.
base rmly into the bottom of the cell body.
Push the ltrate exit tubing onto the exit spout of the 4.
membrane holder.
Page 19
Stirrer Assembly Installation
Place the stirrer assembly into the cell body. When 1.
properly installed, the arms of the stirrer assembly
will rest on the small ridge inside the top of the cell
body.
Pour the sample into the cell.2.
Push the cap assembly down onto the cell body, 3.
using a twisting motion and orienting the gas inlet
port on the cap opposite the ltrate exit port on the
holder.
NOTE: If the cap assembly does not slide easily,
lubricate the O-ring lightly with water or
petroleum jelly. To avoid clogging the
membrane pores, do not allow petroleum
jelly to contact the membrane.
Set the pressure-relief valve knob to the horizontal 4.
(open) position.
Slide the cell into the retaining stand, tting the ring 5.
on the cell base into the hole in the stand. The
attened edge on the bottom ange of the cap
ensures that the cell is inserted properly and
prevents rotation of the cell once inside the stand.
Turn the pressure-relief valve knob to the vertical 6.
(closed) position.
15Stirred Ultraltration Cells
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Gas Pressure Line Installation
Attach the gas pressure line to the tube tting on the
cap as follows:
Unscrew the hexagonal nut (a) from the cap assem-1.
bly and remove the O-ring (d).
½”
a
b
c
d
Slide the hex nut onto the end of gas tubing. 2.
Slide the metal grab ring (b) down one half-inch onto 3.
the tubing, with the ange facing away from the hex
nut.
Add the white spacer (c), positioning the counter-4.
sunk surface toward the ange on the grab ring.
Slide the O-ring (d) onto the end of the tubing. 5.
Insert the exposed tubing into the cap assembly and 6.
hand-tighten the hexagonal nut. Do not overtighten.
Page 21
Operating Guidelines
See membrane instructions for cleaning and storage ■
procedures, and for buffer and solvent compatibility.
Highly viscous solutions lter slowly, as do solutions ■
containing particulate matter, such as colloids.
Where a viscous agent (sucrose, glycerin, etc.)
is to be removed, ow can often be increased by
pre-dilution.
Prelter or centrifuge any solution containing par- ■
ticulate matter, such as cell debris or precipitates.
During extended dialtration, liquid level may ■
increase slightly. To correct, concentrate briey.
To maximize recovery of retained substances, ■
continue stirring for a few minutes after
depressurization. This will resuspend the polarized
layer at the membrane surface.
CAUTION: Always check that the pressure-relief
valve knob is set to the horizontal (open)
position, before removing the cell top.
Removal of the top with the pressurerelief valve knob in the vertical (closed)
position can create a partial vacuum,
which can rupture the membrane.
17Stirred Ultraltration Cells
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Limitations
This section explains the limitations of the stirred ultraltration cell.
Pressure Limits
Do not exceed 75 psi (5.3 kg/cm2) nitrogen gas pressure.
CAUTION: Never operate the cell without its
retaining stand. The cap will pop off when
the cell is pressurized, and the liquid in
the cell may spill or splatter.
Temperature Limits
Although brief exposure to higher temperatures is
possible, do not operate cell continuously above 85 °C
(185 °F).
Chemical Resistance
Do not use the stirred cell with:
Strong acids (pH < 2) or strong alkalies (pH > 10)
Ketones (including acetone)
Aromatic hydrocarbons (including toluene)
Cellosolve
Halogenated hydrocarbons
DMF
Aliphatic esters
DMSO
Polar aromatics
NOTE: The spring in the pressure-relief valve is NOT
®
solvent
compatible with 0.1 N NaOH.
Page 23
Chemical Resistance, continued
For other solvent compatibilities, consult a standard
text or contact Millipore Technical Service. For the
chemical resistance of disc membranes, see the
instructions packed with the product.
Cell Operation
Select the cell operating mode as follows:1.
For concentration: Connect the inlet line to a
regulated gas pressure source.
NOTE: Use nitrogen gas for pressurizing the cell.
Using compressed air can cause large pH
shifts, due to dissolution of carbon dioxide.
With sensitive solutions, oxidation may also
occur, potentially leading to other problems.
For dialtration: Connect the cell to an auxiliary
reservoir, using the Concentration/Dialysis
Selector Valve, model CDS10 (Millipore
cat. no. 6003). Refer to User Guide 99104
for detailed instructions.
Hold the cell steady on the laboratory bench and 2.
pressurize. Follow the instructions in the membrane
package to determine optimal operating pressure.
Do NOT exceed the cell pressure limit of 75 psi
(5.3 kg/cm
cap assembly moves upward, forming a secure lock
with the retaining stand.
2
). Once the system is pressurized, the
19Stirred Ultraltration Cells
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Cell Operation, continued
▲WARNING: When operating with hazardous
or especially valuable materials,
pressure-check the cell
thoroughly, to ensure that all
components have been properly
assembled, before turning on
the stirring table.
Place the cell on the magnetic stirring table.3.
To avoid membrane damage, make sure that the cell 4.
is pressurized before turning on the stirring table.
Turn on the stirring table and adjust the stirring rate, 5.
until the vortex created is approximately one-third
the depth of the liquid volume.
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21Stirred Ultraltration Cells
Shutting Down the System
Turn off the stirring table and then turn off the 1.
nitrogen pressure source.
CAUTION: In the next step, overly rapid
depressurization can cause the
membrane to buckle up and rupture.
Slowly vent the pressure inside the cell, by turning 2.
the pressure-relief valve knob to the horizontal
position. Push the cap down, and then slide the cell
out from the retaining stand.
With a twisting motion, remove the cell cap and the 3.
magnetic stirrer assembly. Pour out the solution.
Disassemble the cell, wash all components with a 4.
mild detergent/water solution and rinse thoroughly.
Sterilization
Millipore stirred ultraltration cells can be autoclaved
at 121 °C (250 °F) for 30 minutes. They are compatible
with standard sterilizing gas mixtures, 70% ethanol,
isopropanol and 5% formalin. The tubing is NOT
autoclavable.
CAUTION: To avoid pressure build-up in the body,
which could result in damage such as
cracking, partially tighten the base before
autoclaving.
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Storage
Disassemble the ultraltration cell whenever it is
unlikely to be used for several weeks.
Maintenance
Replace O-rings at the rst sign of damage or wear. ■
If installation or removal of the cap assembly ■
becomes difcult, lubricate the cap O-ring with a
small amount of petroleum jelly or water.
CAUTION: To ensure proper ltration, do not allow
petroleum jelly to come in contact with
the membrane.
Periodically inspect the transparent body for cracks ■
and inspect the stirrer assembly for washer wear or
rough edges, which could damage the membrane.
CAUTION: Do not use cell if the transparent body
is cracked or crazed.
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23Stirred Ultraltration Cells
Troubleshooting
Symptom: Little or No Filtrate Obtained
Rotate the pressure-relief valve knob to check for ■
pressure. If the cell is not pressurized, check the
nitrogen source and regulator.
Make sure the glossy side of the disc membrane faces ■
up.
If the sample solution is highly viscous, due to microso- ■
lutes, either dilute or dialter to increase ow rate.
Check the membrane holder and ltrate port for ■
blockage.
Symptom: Filtration Rate Abnormally High
Check the membrane for lesions, scratches and rough- ■
ness.
Make sure that the correct membrane type is being used. ■
Make sure cell is pressurized ■ prior to placing onto
magnetic stirring table.
Check the stirrer assembly to ensure that the stirring ■
bar is not contacting the membrane surface.
Symptom: Cell Leaks
Be sure that the lower O-ring rests entirely on the ■
peripheral surface of the membrane.
Check the O-rings for nicks or cuts. ■
Make sure that the membrane support is seated ■
properly, and that the base is screwed in rmly.
Make sure that the O-rings have not been not squeezed ■
out of their slots.
Check the ttings on the gas inlet tubing for correct ■
order and position. See the Gas Pressure Line
Installation section of this user guide.
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Specications
Model No. 8003 8010 8050 8200 8400
Cell capacity (mL) 3 10 50 200 400
Minimum process
volume (mL)
Membrane diam. (mm) 25 25 44.5 63.5 76
Effective membrane
area (cm
Hold-up volume
2
)
1
Empty weight,
approx. (g)
Retaining stand base
dimensions (cm)
Retaining stand
height (cm)
Maximum operating pressure: 75 psi (5.3 kg/cm
Relief valve setting: 90 psi (6.3 kg/cm
Maximum dialtration operating pressure: 55 psi (3.9 kg/cm
1
Non-recoverable volume (below membrane surface)
Materials of Construction
Cap: nylon
Membrane holder: polysulfone
Body: polysulfone
Magnetic stirrer assembly: acetal, polysulfone
Retaining stand: nylon
O-rings: silicone rubber (red), buna-N copolymer (black)
Tube tting assembly: nylon
0.075 1.0 2.5 5.0 10.0
0.9 4.1 13.4 28.7 41.8
(mL) 0.07 0.2 0.5 1.2 1.5
130 130 220 360 610
6 × 6 6 × 6 7 × 7 9 × 9 11 × 11
7.7 7.7 9.8 12.8 15.5
2
2
)
)
2
)
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25Stirred Ultraltration Cells
Compliance with the Pressure Equipment
Directive, 97/23/EC
Millipore Corporation certies that this product
complies with the European Pressure Equipment
Directive, 97/23/EC of 29 May 1997.
This product is classied under Article 3 § 3 of the
Pressure Equipment Directive. It has been designed
and manufactured in accordance with sound
engineering practice to ensure safe use.
The product is accompanied by user instructions and
bears markings to permit identication of Millipore
Corporation as the manufacturer or authorized
representative of this product within the European
Community.
In compliance with Article 3 § 3 of the Pressure
Equipment Directive, this product does not bear the CE
mark.
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Product Ordering Information
This section lists the catalogue numbers for the Model
8000 series Stirred Ultraltration Cells, accessories, and
replacement parts. See Technical Assistance section for
information about contacting Millipore. To order Millipore
products on-line, go to www.millipore.com/products.
Catalogue
Number
Ultraltration Cell
5125 Model 8003 1
5121 Model 8010 1
5122 Model 8050 1
5123 Model 8200 1
5124 Model 8400 1
Accessories
6003 CDS10 Concentration/Dialysis
6015 MF2 Push-Button Manifold.
6028 RC800 Reservoir. Provides
6022 TA1 Tubing Adapter Kit. Adapts
Description Qty/pk
1
Selector. For instant switching
from concentration to rapid dialysis
(dialtration).
1
For operation of multiple cells or
reservoirs; individually valved.
1
800 mL extra uid volume. Can be
used for rapid dialysis (dialtration).
1
plastic tubing to 1/8” or 1/4” male
or female gas ttings.
Page 31
27Stirred Ultraltration Cells
Product Ordering Information,
continued
R-3603,
®
Tygon
®
I.D. 3/32 (2.4 mm), O.D. 5/32 in. (4mm)]
Replacement Parts
Use the following table to determine which replacement kit to order. Refer to ultraltration cell
assembly diagrams on pages 2–11 for letter designations. The parts contained in each kit are
listed on pages 28—29.
Description Item 8003 8010 8050 8200 8400
Cap A,B 8003SCPKIT 8010SCPKIT 8050SCPKIT 8200SCPKIT 8400SCPKIT
O-ring C 8000SCPKIT 8000SCPKIT 8000SCPKIT 8000SCPKIT 8000SCPKIT
D 8003SCPKIT 8010SCPKIT 8050SCPKIT 8200SCPKIT 8400SCPKIT
Stirrer
assembly
Body E N/A (5125) N/A (5121) N/A (5122) N/A (5123) N/A (5124)
I Fisher cat. no. 14-169-1C [Saint Gobain
H 8000SCPKIT 8000SCPKIT 8000SCPKIT 8000SCPKIT 8000SCPKIT
O-ring F 8000SCPKIT 8000SCPKIT 8000SCPKIT 8000SCPKIT 8000SCPKIT
Membrane
Holder
Elastomeric
Base J 8003SCPKIT 8010SCPKIT 8050SCPKIT 8200SCPKIT 8400SCPKIT
Tube Fitting
tubing
8000SCPKIT 8000SCPKIT 8000SCPKIT 8000SCPKIT 8000SCPKIT
L
K 8000SCPKIT 8000SCPKIT 8000SCPKIT 8000SCPKIT 8000SCPKIT
assembly
Tubing,
plastic
Stand M N/A (5125) N/A (5121) N/A (5122) N/A (5123) N/A (5124)
O-ring N 8000SCPKIT — — — —
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Product Ordering Information,
continued
Catalogue
Number
8000SCPKIT 8000 Series Stirred Cell Replacement Part
* Refer to ultraltration cell assembly diagrams on pages
2–11 for letter designations.
Item* Description Qty/pk
Kit (contains the following parts for Models
8003, 8010, 8050, 8200, 8400)
C Cap O-Rings
Models 8003, 8010 2
Model 8050 2
Model 8200 2
Model 8400 2
F Membrane holder O-Rings
Models 8003, 8010 2
Model 8050 2
Model 8200 2
Model 8400 2
N Membrane holder O-Ring for
Model 8003
H Membrane Holders
Model 8003 1
Model 8010 1
Model 8050 1
Model 8200 1
Model 8400 1
K
Tube Fitting Assembly 1
L
Tubing, plastic 10 ft
2
Page 33
29Stirred Ultraltration Cells
Product Ordering Information,
continued
Catalogue
Number
8003SCPKIT Model 8003 Replacement Parts Kit
8010SCPKIT Model 8010 Replacement Parts Kit
8050SCPKIT Model 8050 Replacement Parts Kit
8200SCPKIT Model 8200 Replacement Parts Kit
8400SCPKIT Model 8400 Replacement Parts Kit
* Refer to ultraltration cell assembly diagrams on pages
2–11 for letter designations.
Item* Description Qty/pk
(contains the following components)
A, B Cap Assembly 1
D Stirrer Assembly 1
J Base 1
(contains the following components)
A, B Cap Assembly 1
D Stirrer Assembly 1
J Base 1
(contains the following components)
A, B Cap Assembly 1
D Stirrer Assembly 1
J Base 1
(contains the following components)
A, B Cap Assembly 1
D Stirrer Assembly 1
J Base 1
(contains the following components)
A, B Cap Assembly 1
D Stirrer Assembly 1
J Base 1
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Technical Assistance
For more information, contact the Millipore ofce nearest you.
In the U.S., call (1-800-645-5476). Outside the U.S., see your
Millipore catalogue for the phone number of the ofce nearest
you or go to our web site at www.millipore.com/ofces for
up-to-date worldwide contact information. You can also visit
the tech service page on our web site at www.millipore.com/
techservice.
Standard Warranty
Millipore Corporation (“Millipore”) warrants its products will
meet their applicable published specications when used in
accordance with their applicable instructions for a period of
one year from shipment of the products. MILLIPORE MAKES
NO OTHER WARRANTY, EXPRESSED OR IMPLIED.
THERE IS NO WARRANTY OF MERCHANTABILITY OR
FITNESS FOR A PARTICULAR PURPOSE. The warranty
provided herein and the data, specications and descriptions
of Millipore products appearing in Millipore’s published
catalogues and product literature may not be altered except
by express written agreement signed by an ofcer of Millipore.
Representations, oral or written, which are inconsistent with
this warranty or such publications are not authorized and if
given, should not be relied upon.
In the event of a breach of the foregoing warranty, Millipore’s
sole obligation shall be to repair or replace, at its option,
the applicable product or part thereof, provided the
customer noties Millipore promptly of any such breach. If
after exercising reasonable efforts, Millipore is unable to
repair or replace the product or part, then Millipore shall
refund to the customer all monies paid for such applicable
product or part. MILLIPORE SHALL NOT BE LIABLE
FOR CONSEQUENTIAL, INCIDENTAL, SPECIAL OR
ANY OTHER INDIRECT DAMAGES RESULTING FROM
ECONOMIC LOSS OR PROPERTY DAMAGE SUSTAINED
BY ANY CUSTOMER FROM THE USE OF ITS PRODUCTS.
Page 35
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