MILESTONE
HELPING
PATIENTS
PRESTO PRO
Automatic processor/stainer
for next generation frozen sections
Operator Manual - MM136
PRESTO PRO MM136-002 – Operator Manual
Thank you for choosing one of our systems and welcome to the ever more crowded club of Milestone laboratory
instrumentation users.
We feel confident that you will be fully satisfied adding this new device to your laboratory.
We recommend to ready carefully this operator manual and to always keep it within reach for convenient and
quick referencing.
For any clarification or request for assistance, please contact our representative in your country:
Or contact:
Milestone s.r.l.
Via Fatebenefratelli, 1/5
24010 Sorisole (BG) Italy
Tel. +39.035.412 8264
Fax +39.035.575498
web site www.milestonemed.com
e-mail marketing@milestonemedsrl.com
Read carefully this manual before using the instrument.
MM136-002 – PRESTO PRO – Operator Manual
2018-11
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PRESTO PRO MM136-002 – Operator Manual
CONTENTS
CONTENTS ....................................................................................................................................................... 4
1. INTRODUCTION ........................................................................................................................................ 6
1.1. Symbols used .................................................................................................................................... 6
1.2. Designated uses ................................................................................................................................ 7
1.3. Technical specifications ..................................................................................................................... 7
1.3.1.
Touch control terminal ............................................................................................................... 7
1.4. Transport and storage conditions ...................................................................................................... 8
1.5. Warning information ........................................................................................................................... 9
1.6. Label ................................................................................................................................................ 10
1.7. Compatible reagents ........................................................................................................................ 10
1.8. Waste disposal ................................................................................................................................ 11
2. INSTALLATION ........................................................................................................................................ 12
2.1. Space requirements ......................................................................................................................... 12
2.2. Unpacking and check list ................................................................................................................. 13
2.3. Fume extraction system ................................................................................................................... 16
2.4. Water fitting ...................................................................................................................................... 17
2.4.1.
Water drain .............................................................................................................................. 18
2.5. Electrical installation ........................................................................................................................ 19
2.5.1.
Power supply ........................................................................................................................... 19
2.6. Final placement ............................................................................................................................... 20
2.7. General description .......................................................................................................................... 20
2.7.1.
Remove or place the reagent cover disc ................................................................................. 21
2.7.2.
Add protection labels to the disc covering the reagents .......................................................... 22
2.7.3.
Cover of the second loading position ...................................................................................... 22
2.7.4.
Loading positions of the racks on the arm of the PRESTO PRO ............................................ 23
2.7.5.
Remove or replace the caps to the reagent vessels ............................................................... 23
2.7.6.
Remove or place the tray of reagent vessels .......................................................................... 24
2.7.7.
Reagent positions inside PRESTO PRO ................................................................................. 25
2.7.8.
Connect the barcode reader .................................................................................................... 26
2.7.9.
Rear panel ............................................................................................................................... 27
2.8. Remote alarm connection ................................................................................................................ 28
2.9. Reagents.......................................................................................................................................... 28
2.10. Accessing and exiting the system ................................................................................................... 29
2.11. Create a new user ........................................................................................................................... 30
2.12. Changing the language .................................................................................................................... 32
2.13. Set Date and Time ........................................................................................................................... 33
2.14. Fill the FineFIX reagent in the PRESTO PRO ................................................................................. 33
2.14.1. Load the consumable changes ................................................................................................ 33
2.14.2. Sequence of software activation .............................................................................................. 34
2.15. Load the reagents in the PRESTO PRO vessels ............................................................................ 35
2.15.1. Labels ...................................................................................................................................... 35
2.15.2. Load fixation, staining and cleaning reagents in the PRESTO PRO ....................................... 36
2.15.3. FineFIX heating settings .......................................................................................................... 40
2.16. Most used programs ........................................................................................................................ 42
2.17. Owner Settings ................................................................................................................................ 44
3. USE PRESTO PRO.................................................................................................................................. 46
3.1. Loading slides in the rack ................................................................................................................ 46
3.2. Run a program ................................................................................................................................. 46
3.2.1.
Run a favourite standard protocol............................................................................................ 48
3.2.2.
Run a favourite “Continuous” protocol ..................................................................................... 51
3.2.3.
Press the start button to run a standard protocol .................................................................... 57
3.2.4.
Press the start button to run a “Continuous” protocol .............................................................. 59
3.3. Editing a program ............................................................................................................................ 60
3.3.1.
Modify a standard protocol ...................................................................................................... 60
3.3.2.
Modify a “Continuous” protocol ................................................................................................ 62
3.3.3.
Modify the temperature of the fixation reagent ........................................................................ 63
4. REAGENT MANAGEMENT ..................................................................................................................... 64
4.1. Use of FineFIX ................................................................................................................................. 64
4.2. Reuse of reagents ........................................................................................................................... 66
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PRESTO PRO MM136-002 – Operator Manual
4.3. Setting the configuration of reagents ............................................................................................... 68
4.4. Edit reagents list .............................................................................................................................. 69
5. SAFETY PROCEDURE ........................................................................................................................... 70
5.1. Water error ....................................................................................................................................... 70
5.2. Arm position lost error ...................................................................................................................... 73
6. MAINTENANCE ....................................................................................................................................... 76
6.1. Introduction ...................................................................................................................................... 76
6.2. Before/after every process ............................................................................................................... 76
6.3. Daily ................................................................................................................................................. 77
6.4. Weekly ............................................................................................................................................. 77
6.5. Yearly ............................................................................................................................................... 77
7. DECONTAMINATION AND CLEANING .................................................................................................. 78
7.1. Safety precautions ........................................................................................................................... 78
7.2. Procedure for PRESTO PRO decontamination and cleaning ......................................................... 78
7.2.1.
Detergents ............................................................................................................................... 78
7.2.2.
Procedure ................................................................................................................................ 79
8. REPORTING ............................................................................................................................................ 81
8.1. Exporting saved process data ......................................................................................................... 81
8.1.1.
How to delete a saved process................................................................................................ 82
8.2. Exporting the events log .................................................................................................................. 83
9. OPTIONALS ............................................................................................................................................. 84
9.1. External exhaust fume filter kit (Code 61550/F) .............................................................................. 84
9.2. FineFIX tanks ................................................................................................................................... 84
A.
Appendix – Hints and tips .................................................................................................................... 85
A.1. Fatty tissues ..................................................................................................................................... 85
A.2. Tips to modify the staining protocol ................................................................................................. 85
B.
Appendix – Alarm management .......................................................................................................... 86
B.1. Alarm management ......................................................................................................................... 86
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PRESTO PRO MM136-002 – Operator Manual
1. INTRODUCTION
1.1. Symbols used
The instructions marked with this symbol are important and must be read carefully.
An instruction accompanied by this WARNING symbol means you need to proceed with
caution. Failure to follow the instruction may endanger the user or cause damage to the
device.
When an instruction features this symbol, great care is required as there is the risk of
an electric shock. Carefully follow the instruction in order to avoid this kind of accident.
Biohazard: pay attention when performing a procedure marked with this symbol, as
there is a biological contamination hazard.
Pay attention: parts identified with this symbol are areas at risk of crushing hand and
or fingers.
Surfaces identified by this symbol feature a high temperature hazard.
Inflammable material.
CE Logo: this instrument complies with European Community directives.
IVD medical device according to directive 98/79/EC.
Symbol of the European WEEE directive (2002/96/EC):
electric/electronic material – do not disperse in the environment.
Fragile
Manufacturer.
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PRESTO PRO MM136-002 – Operator Manual
1.2. Designated uses
Milestone PRESTO PRO and its optional parts have been designed for laboratory use and are therefore able
to withstand the harshest lab conditions. They have been developed for continuous use in procedures for
fixation, processing and staining of specimens for extemporaneous diagnoses in a quick, reliable and
standardized manner.
Milestone PRESTO PRO must solely be used with the reagents listed in the “Compatible reagents” chapter,
refer to chapter 1.7.
Any other use of the instrument should be deemed as misuse and voids the manufacturer's
warranty.
1.3. Technical specifications
REF 100300: PRESTO PRO 230V~ 50/60Hz 180W
REF 100301: PRESTO PRO 115V~ 60Hz 180W
Productivity: 120 slides per hour
194 slides per hour with loading “Continuous”
Rack capacity: 6 slides
Number of stations: 10
Reagent vessel volume: 200ml
Operating Temperature: 18°C – 30°C (64.4°F – 86°F)
Humidity (relative): up to 80% non-condensing
Maximum altitude: 2000m (6561ft)
Air intake flow interval: 35-45m3/h (cubic meters per hour).
Weight: 42Kg (92lb)
The instrument is intended for laboratory use (indoor use) only.
1.3.1. Touch control terminal
The following picture shows the Touch Control Terminal of the PRESTO PRO unit. It is possible to operate the
unit simply by touching the icons on the screen.
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PRESTO PRO MM136-002 – Operator Manual
Two USB ports are at the sides of the Touch Terminal.
When the USB ports are not used, it is required to leave the port protection on, as shown
in the picture above.
Characteristics: TFT-LCD 7” widescreen color display
Resolution 800X480,
Resistive Touchscreen
Mass storage 4GB
CPU 1GHz
Windows™ Embedded Compact 7
Programs: Pre-stored and customizable user defined programs.
I/O-Interface: 2x USB ports 2.0, 1x LAN Ethernet 10/100 Mbps RJ-45.
The manufacturer reserves the right to change and/or improve the specifications with no
prior notice and without incurring any obligation.
1.4. Transport and storage conditions
Temperature: –20°C up to +60°C (-4°F up to 140°F)
Humidity: up to 80% (up to 50% at 40°C) (104°F) non-condensing.
Before switching on PRESTO PRO (when coming from a storage chamber), allow the
instrument to reach operating environment conditions (at least half an hour).
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PRESTO PRO MM136-002 – Operator Manual
It is important that normal standards for safety and good laboratory practices are
1.5. Warning information
Any use of the instrument other than specified forfeits the warranty provided by the manufacturer.
PERSONAL DATA
The Device described herein is not intended for managing personal data of natural persons. Personal data of
natural persons shall not be inserted into the Device during the use of the Device. The organization that uses
the Device shall implement appropriate measures to ensure that no personal data of natural persons are
inserted into the Device. All the data referred to a person and inserted into the device shall use, at least, the
pseudonymization, in such a manner that the personal data can no longer be attributed to a specific data
subject without the use of additional information, provided that such additional information is kept separately
and is subject to technical and organizational measures to ensure that the personal data are not attributed to
an identified or identifiable natural person.
The main power supply plug is to be considered as disconnect device. Disconnect all plugs from the power
outlet before assembling the system, before connecting optional parts (if any) and before cleaning it.
The equipment must be connected to well visible sockets and accessible by the operator.
The instrument is supplied with replaceable fuses, but their replacement must be effected by trained and
authorized SERVICE personnel. If the system is still not properly working, please contact the local authorized
and trained SERVICE representative or the manufacturer.
All parts of the equipment and its optional parts shall be supplied only by the manufacturer.
DO NOT OPEN THE UNIT'S BODY. Failure to comply with the instructions in the
documentation might lead to a reduction in the device's safety. Should the equipment
not work correctly you are kindly requested to contact the supplier or manufacturer.
Do not remove the instrument, optional parts, panels or cover. Only authorized and
qualified personnel may repair and access the instrument's internal components.
applied. Always use common sense and the best-known laboratory practice when
operating the instrument.
The Competent Authority who owns the instrument has the primary responsibility for
incidents due to operations on the instrument without attending personnel in charge
of use or repairs.
Only use the reagents listed in chapter 1.7 “Compatible reagents”, to avoid damage
to the instrument. Failure to follow the instructions might lead to decreased device
safety.
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PRESTO PRO MM136-002 – Operator Manual
Fixative
Staining
Cleaning
1.6. Label
MILESTONE SRL
Via Fatebenefratelli, 1/5 – 24010 Sorisole (BG) – ITALY
Tel. +39.035.4128264
Fax. +39.035.575498
PRESTO PRO
REF 100300
SERIAL NUMBER (SN)
INPUT
MANUFACTURED:
For an explanation of symbols see chapter 1.1.
1.7. Compatible reagents
reagents
Milestone
FineFIX
Limonene
Reagents
Haematoxylin
Eosin
Reagents
Ethanol >99.9%
(Ethanol)
Acetic Acid 2% in
water
(Acetic Acid 2%)
Xylene
(Xylene)
Isoparaffin
(Isoparaffin,
MileGREEN)
Isopropanol
(Isopropanol)
DO NOT USE REAGENTS OTHER THAN THOSE LISTED ABOVE.
In case of doubts please contact: application@milestonemedsrl.com.
DO NOT USE HYDROCHLORIC ACID in any concentration.
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PRESTO PRO MM136-002 – Operator Manual
Handle with care and st
ore in a cool dry space using a tightly closed container.
1.8. Waste disposal
This instrument is a medical in-vitro device and is usually installed in a laboratory where are specimens and
other biological tissue. For your safety, it is therefore required to clean and disinfect the instrument prior to
coming into contact with it. It is also required to wear gloves when operating the system.
In the event this instrument should be returned to Milestone S.r.l., it is required to clean and disinfect the unit
before shipping it. Non-disinfected devices shall not be accepted and you will be contacted to pay the relative
disinfection costs.
International health standards require shipments of biological hazard material to be effected in standard
containers (risk of sanctions).
Do not remove the frame panels of the unit during cleaning operations.
Disconnect the power supply before any operation.
Do not use direct or high-pressure water to clean the unit.
For safety reason, any operation without the personal protective equipment must be
avoided.
It is recommended to use polyphenolic based disinfectants to clean and disinfect the instrument.
For further information, please contact the manufacturer: application@milestonemedsrl.com.
Complying with directive 2012/19/EC of the European Parliament and of the Council of 4 July 2012 on waste
electrical and electronic equipment (WEEE), the separate collection environmental managed of equipment is
mandatory.
It is necessary to return the used equipment to the distributor or to inquire about the presence of a local
empowered system for collection and disposal of WEEE.
The in-observance of Directive 2012/19/EC or of the local law which acknowledge it can have potential effects
on the environment and human health.
This symbol indicates separate collection
for electrical and electronic materials
If additional requirements on accident prevention and environmental protection exist in the country of
operation, this instruction manual must be supplemented by appropriate instructions to ensure compliance to
such requirements.
Waste disposal of the reagents
Vapours may collect in empty containers. We therefore recommend treating empty
containers as hazardous waste.
Waste material should be disposed of in an approved incinerator or in a designated landfill
site, in compliance with all provincial and local government regulation.
Certain reagents may have effects on health and cause environmental pollution.
For further information please refer to the SDS (Safety Data Sheet) provided by your supplier.
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PRESTO PRO MM136-002 – Operator Manual
20cm
20cm
20cm
44cm
50cm
2. INSTALLATION
Milestone PRESTO PRO is an instrument that requires the utmost attention when unpacked and installed.
Arrange a bench at least 84cm (33in) wide and 70cm (27.5in) deep able to withstand the weight of PRESTO
PRO which is 42Kg (92.5 lbs).
Then place PRESTO PRO on the bench, ensuring the unit is level and the table is not flammable and vibration
proof. Ensure the instrument is positioned away from heat sources or from direct sunlight. Finally, ensure the
box collecting excess reagents is in the proper position.
Ensure the unit is not placed against any side surface thus restricting intake/outlet cooling
air: leave at least a 20cm (7.9in) distance on each side.
Maximum height 860 mm
Height 610 mm
Width 440mm Depth 525mm
2.1. Space requirements
PRESTO PRO is shipped in a wooden case with the following dimensions:
Width: 580mm (23in) Height: 1000mm (39.4in) Depth: 1050mm (41.3in)
Unit dimensions:
Width: 440mm (17.3in) Height: 610mm (24in) Depth 525mm (20.7in)
Dimensions of the space required to place the unit
Width: 840mm (33in) Height: 900mm (35.4in) Depth: 725mm (28.5in)
The MINIMUM depth required for the bench is 725mm (28.5in).
PRESTO PRO is a heavy instrument. Ensure the bench can withstand it.
At least two people are necessary to move and unpack the instrument.
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PRESTO PRO MM136-002 – Operator Manual
2.2. Unpacking and check list
On opening the package ensure all parts are present by checking the list attached with the shipment. The
utmost attention must be paid during PRESTO PRO unpacking to prevent scratches or damage to the body.
The PRESTO PRO system (REF 100300 and REF 100301) includes the following components:
• Qty.1 Main power supply cable (Code 50036 for 230V version)
• Q.ty. 1 Exhaust tube with 2 metal clamps (Code 61410)
• Qty. 1 20µm water filter (Code 100180L) with two G 1/2’’ connectors (Code 68278)
• Qty. 2 Water pipe 2m (Code 68309)
• Qty. 3 Electro-polished steel rack for 6 slides (Code 100177)
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PRESTO PRO MM136-002 – Operator Manual
Code
100323
Code 100212
• Qty. 10 Steel reagent container (Code 100197). Eight containers are labelled and designated for the
positions in the PRESTO PRO. The other two are for transporting the slides from the cryostat and the
stained ones to their place of assembly.
• Qty. 1 Aluminum vessel for heated reagents (Code 100344)
• Qty. 1 Reagent container cover disc (Code 100375)
Consisting of:
10 protective polyester discs (
Cover of the second position (
)
)
• Qty. 1 Safety screw of the reagent container cover disc (Code 100348)
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PRESTO PRO MM136-002 – Operator Manual
• Qty. 1 Tray for vessels (Code 100367)
• Qty. 1 Kit reagent labels (Code 100356)
White labels
for customization
of reagents
• Qty.1 Magnetic pen to work with the touch screen terminal (Code 50597/M)
• Qty.1 Operation manual in electronic form
• Q.ty 1 Linear barcode reader (Code 370059)
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PRESTO PRO MM136-002 – Operator Manual
2.3. Fume extraction system
PRESTO PRO is ventilated through the external exhaust outlet located at the rear of the instrument, with a
pipe connected to the fume extraction system (if any).
The external fume extraction system must be properly installed at all times, according to
local regulations in force on laboratory safety.
The customer is required to adapt the hose connector. The plastic hose supplied has these specifications:
• Length: 3m
• Outer diameter: 69mm
• Inner diameter: 60mm
The external fume extraction system must assure an air flow of 40m3/h
(cubic meter per hour).
There is no filter on the fume extraction system of the unit.
it is possible to connect an external exhaust fume extraction kit (Code 61550/F), please refer
to chapter 9.1.
Connect the exhaust tube (Code 61377) to the appropriate outlet on the back panel.
Use the metal strap to fasten the pipe to the fume exhaust duct. (Code 50051).
Now connect the other end of tube to the fume extractor of the laboratory.
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PRESTO PRO MM136-002 – Operator Manual
It is mandatory to install the water filter between the tap and the
water inlet in the PRESTO
2.4. Water fitting
The PRESTO PRO unit is to be connected to the water system in order to work properly. On the back of the
unit are the ports for water inlet (left) and drain (right), pictured below.
Connect the (cold) water pipe to the water inlet at the rear of the unit. Gradually open the water "supply".
Connect the drain pipe to the water outlet at the rear of the unit.
PRO. The filter prevents the formation of limestone and dirt in the water system of
PRESTO PRO.
The filter fittings are G 1/2’’.
In order to simplify the replacement of the filter cartridges, it is important to have a tap
upstream of the filter, as pictured below.
Milestone recommends working in cooperation with the hospital’s clinical engineering
department when installing the water system of PRESTO PRO.
Water connections:
Water inlet: connection for a 17mm pipe (with G 1/2’’ fitting)
Water outlet: connection for a 17mm pipe (with G 1/2’’ fitting)
The minimum difference in height between the water outlet and the water system is at
least 50cm (19.7in), otherwise the system does not drain water properly.
Ensure the drain pipe has a slope of approximately 10cm (4in) for every meter.
Incoming water pressure: maximum 2bar, minimum 1bar
Milestone recommends using a water softener.
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PRESTO PRO MM136-002 – Operator Manual
2.4.1. Water drain
The drain water is polluted with the reagents in which the rack is dipped before rinsing.
The amount of reagent that a fully loaded rack (6 slides) takes in is about 1 – 1.5ml. Therefore, the drain water
of a standard process, with water flow duration 30 seconds, contains about 1.5ml FineFIX, haematoxylin and
2% acetic acid. In other words, the concentration of these substances in the drain water is about 0.075%
FineFIX and haematoxylin and 0.0015% of acetic acid.
The waste water may contain pathogens and must be connected according to local
regulations.
In the interest of safety, the operator must be present throughout the entire process.
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PRESTO PRO MM136-002 – Operator Manual
Before connecting the plug to the laboratory’s socket, check that the power supply voltage
2.5. Electrical installation
2.5.1. Power supply
The power supply line needs to be grounded. The earthing connection of the
power supply socket must be designated and come directly from the electrical distribution board of the
laboratory.
Carefully ascertain that:
• There is an earthed connection in the laboratory. Do not connect Milestone units without earthing
connection.
• The power cords (size and distance from the electric board) are compliant with the label on the back
of the unit.
• The socket is visible and accessible.
The power supply line needs to be grounded (Class I device).
Power line protection:
115V version: Miniature circuit breaker curve C, 15A, residual current device: 30mA.
230V version: Miniature circuit breaker curve C, 10A, residual current device: 30mA.
A power cord is supplied with Schuko plug.
of the unit matches the power supply line of the laboratory.
Damage occurs if the instrument is supplied with incorrect power supply.
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PRESTO PRO MM136-002 – Operator Manual
3
1
2
4
5
6
7
8
9
1
2
3
4
5
6
7
2.6. Final placement
The PRESTO PRO unit may now be placed in its final position of use. Ensure the unit is level.
Then connect:
The main power supply cable to the power plug located at the rear of the unit, then plug the power supply
cable into the socket.
Now it is possible to switch PRESTO PRO on.
2.7. General description
1. Work area cover
2. Intake
3. Disc covering the reagents
4. Reagent vessels
5. Tray of the reagent vessels
6. Touch screen terminal
7. USB ports
8. Main power switch
9. Collecting tray (Code 100360) BEA
1. Arm with rack supports
2. Standard rack position (BLUE)
3. Secondary rack position (YELLOW)
4. Reagent containers
5. Water container
6. FineFIX Container
7. Tray of the reagent containers
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PRESTO PRO MM136-002 – Operator Manual
2.7.1. Remove or place the reagent cover disc
Removal of the reagent cover disc is required for reagent change operations and for PRESTO PRO cleaning
and maintenance.
To remove the disc, move the safety screw of the covering disc to the front, moving the arm of the standard
position (BLUE) back (see section 2.7.7).
In case of filling or changing the reagents or for cleaning PRESTO PRO press the icon of the reagent
management page: the software automatically moves the arm in that position (see chapter 2.15).
At this point, loosen the screw and lift the rack support, which allows the covering disc to be extracted as it is
connected to it. Pay attention not to hit the reagent vessels.
Upon completing the operations that required disc removal, always place it back in position.
Always place the disc back in position to prevent excessive evaporation of the reagents
and preserve their cleanliness.
To put the covering disc back in position, place it on the central piston so that the standard position (BLUE) is
at the back; the hole for the safety screw is visible and the hole engages to the base of the arm. The hole in
fact has a semicircular shape with one straight side, which must be positioned in front of the operator. Place
the safety screw and tighten it, while holding the rack support steady.
Always tighten the safety screw completely.
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PRESTO PRO MM136-002 – Operator Manual
2.7.2. Add protection labels to the disc covering the reagents
The cover disc is in direct contact with the reagents and in some cases, it needs greater protection. Some vinyl
labels are supplied together with the reagent cover disc, which make the disc more resistant once they are
applied.
Milestone recommends to reinforce the protection disc in the positions where the following reagents are:
• acids
• alcohol solvents (ethanol, isopropanol)
• xylene and xylene substitutes (isoparaffin, limonene)
For further information, please contact: application@milestonemedsrl.com.
Underneath the disc there are slots to indicate the position for the protection labels. Turn the cover disc over
and apply the label in the appropriate position, following the guide markings.
2.7.3. Cover of the second loading position
The reagent covering disc has a hole at the secondary loading position (marked in yellow) so that it can be
used. This hole can be closed when the protocols are not used continuously, in order to reduce evaporation
of the reagents.
Remove the reagent covering disc and, while holding the arm raised, place the cover on the hole and push it
downwards until the hole is completely covered.
Remove the cap to use the second loading position (yellow).
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PRESTO PRO MM136-002 – Operator Manual
Secondary
Standard
2.7.4. Loading positions of the racks on the arm of the PRESTO PRO
The arm of the PRESTO PRO has two loading positions for racks:
The standard loading position: it is the position used for all the standard protocols and for all the odd
numbered cycles of the “Continuous” protocols, and is marked in blue
The secondary loading position: it is the position used only for even numbered cycles (from the second
cycle onwards) of the “Continuous” protocols, and is marked in yellow.
This manual refers to the standard loading position, in blue, unless specified otherwise.
loading
position
loading
position
2.7.5. Remove or replace the caps to the reagent vessels
The reagent vessels are fitted with a cap that prevents evaporation and oxidation of the reagents. The caps
must be removed before any process and it is suggested to place them back after using PRESTO PRO if it is
not going to be used for a long time (more than 24 hours).
Remove the cap while holding the vessel steady and with a slow and gentle movement to prevent the vessel
from moving and spilling the reagent. After removing the cap clean it from any trace of reagent and set it aside
(for instance, in the space behind the reagent vessel tray).
Place the cap back while holding the container and with a slow and gentle motion paying attention not to spill
out the reagent in the vessel.
It is possible to apply a label with the number on the cap, so as to always use the same cap for each vessel.
Use the Kit of reagent labels (Code 100356).
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PRESTO PRO MM136-002 – Operator Manual
2.7.6. Remove or place the tray of reagent vessels
The support disc of the vessels is required for correct positioning of the reagent vessels.
To remove the support disc (for the purpose of cleaning), remove the reagent protection disc (refer to chapter
2.7.1) and all the reagent containers (do not remove the container in position 3 which is for water).
It is required to bring the PRESTO PRO arm to lower position, preferably in the 5DOWN position with arm
turned backwards (see section 2.7.7). Press the icon of the reagent management page: the software
automatically moves the arm in that position (see chapter 2.15).
Lift the support disc and extract it from the water container and the housing for the fixative container.
Remove the support disc without fitted vessels.
To replace the support disc, insert its central hole in the arm shaft, turn the disc so that the number 10 on the
disc label is in front of the operator and the metal water glass and support for the FineFIX container go through
the side holes.
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PRESTO PRO MM136-002 – Operator Manual
2.7.7. Reagent positions inside PRESTO PRO
The slide rack support may make two movements:
• circular motion to move the rack in one of the 10 reagent positions
• vertical motion to lift the rack for moving from one reagent and lower it for dipping in another. In every
position, the vertical movement also allows to perform:
- dripping to move the rack up and down in the reagent, so as to stir the reagent and achieve
better contact between specimen and reagent.
- dipping to shake the rack in order to remove excess reagent from the slide (this function is
recommended after staining reagents).
There are 10 positions inside the PRESTO PRO and they are numbered clockwise:
• Position 10: position immediately in front of the operator. It may be:
- 10UP, PRESTO PRO reset position
- 10DOWN, standby position where the cover disc closes the reagent vessels to prevent
evaporation.
• Position 1 In this position the reagent vessel is designated for FineFIX for specimen processing. This
is the only heated position of PRESTO PRO.
Position 1DOWN with arm lowered is the loading position of the rack at the start of a process.
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PRESTO PRO MM136-002 – Operator Manual
• Position 3: This position is fixed and designated for the rinse water reagent, the flow of which is
regulated automatically.
• Position 5DOWN Position taken for reagent change that assures simple removal of the cover disc.
• Positions 2, 4, 5, 6, 7, 8, 9 and 10: these positions are designated for the staining and cleaning
reagents. The reagents set by default in these positions are those used in the default HIGH QUALITY
program recommended by Milestone (refer to chapter 3.2). The reagents in these positions are not
fixed and may be set by the user, refer to chapter 4.3 Setting the configuration of reagents .
2.7.8. Connect the barcode reader
Connect the reader’s USB to one of the USB ports on the right side of PRESTO PRO.
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PRESTO PRO MM136-002 – Operator Manual
1
2
3
4
5
6
7
8
2.7.9. Rear panel
The following are found on the PRESTO PRO rear panel:
1. Connection to the fume extraction system
2. External alarms connector
3. Ethernet/LAN connection
4. General fuses
5. Spare fuses
6. Power plug
7. Label with serial number
8. Water inlet/outlet connections
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PRESTO PRO MM136-002 – Operator Manual
Reagent REQUIRED for operation: FineFIX Concentrate (5L tank: REF: 70148 or 10L
Please refer to manual MM029 for proper usage and dilution of FineFIX.
Handle reagents with care and store in a cold dry space using a tightly closed container.
2.8. Remote alarm connection
PRESTO PRO is also fitted with connection for external alarm devices.
Output Contact Status
1. Identifies the alarm status of the
PRESTO PRO
…
2. External pump command,
24Vdc 1A max output
Output 2, for the external pump, can be used only for demonstration purposes. It is strictly forbidden to use the
contact for auxiliary pumps during operation with histological samples.
For safety reasons and to prevent damages to the internal relays, the voltage of the
external devices that are connected to PRESTO PRO must be lower than 24 VDC - 1A.
See also the equipment's label which provides some important information.
Always provide the unit's serial number to Customer Support.
1 Normally OPEN
2 COMMON alarm
7 +0Vdc 1A max Pump
8 +24Vdc 1A max Pump
2.9. Reagents
Complete the set up with reagents, by providing at least:
FIXATION/PROCESSING REAGENTS:
• 200mL of diluted FineFIX
tank: REF: 70149) is not included in the unit, to be ordered apart.
STAINING REAGENTS:
• 200mL Hematoxylin
• 200mL Eosin
CLEANING REAGENTS:
• 200mL of Isopropanol
• 200mL 2% Acetic Acid in water
• 400mL 100% Ethanol (2 vessels).
The above reagents must be on site and available at the time of unit installation.
Vapours may build up in the vessels. Treat empty vessels as hazardous.
Waste reagents are to be disposed of in suitable incinerators, in compliance with all local,
regional and state laws.
For further information please refer to the SDS (Safety Data Sheet) provided by the
reagent’s supplier.
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