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Mettler Toledo
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UV5
User Manual
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Mettler Toledo UV7, UV5, UV5Bio User Manual

User Manual
UV/VIS Excellence
UV7/UV5/UV5Bio

Table of Contents

1 Introduction 3
2 Safety information 4
2.1 Definition of signal words and warning symbols ................................................. 4
2.2 Product specific safety notes ............................................................................. 4
3 Design and Function 6
3.1 Type definition and compatibility ....................................................................... 6
3.2 Overview ........................................................................................................ 6
3.3 Rear panel connections .................................................................................... 7
3.4 User Interface .................................................................................................. 8
4 Installation 12
4.1 Scope of delivery ............................................................................................. 12
4.2 Unpack the spectrophotometer .......................................................................... 12
4.3 Position the spectrophotometer.......................................................................... 13
4.4 Connecting the terminal.................................................................................... 13
4.5 Connect the spectrophotometer to the power supply............................................. 13
4.6 Installing cuvette holder and inserting a cuvette................................................... 14
5 Operating the Instrument 16
5.1 Start up and shut down the spectrophotometer .................................................... 16
5.2 Performing a measurement............................................................................... 16
5.3 Methods ......................................................................................................... 17
5.4 Direct measurement ......................................................................................... 20
5.5 Create and handle shortcuts.............................................................................. 26
6 Maintenance and Care 29
6.1 Cleaning cuvette holders and cuvettes................................................................ 29
6.2 Cleaning the housing ....................................................................................... 29
6.3 Transporting the instrument............................................................................... 30
7 Disposal 31
8 Technical Data 32
8.1 Spectrophotometer ........................................................................................... 32
8.2 Measurement .................................................................................................. 32
8.3 Terminal ......................................................................................................... 33
Table of Contents 1UV/VIS Excellence
Table of Contents2 UV/VIS Excellence

1 Introduction

Thank you for choosing a METTLER TOLEDO UV/VIS Excellence spectrophotometer. The UV/VIS Excellence spectrophotometer is an easy-to-operate instrument for measuring molecular absorbance or transmittance in the ultra-violet (UV) and visible (VIS) range of analytical samples.
About this document
This document provides you with the information you need to get started with your METTLER TOLEDO spectrophotometer.
For a comprehensive description of the spectrophotometer and its functions, refer to the Operating Instructions.
The instructions in this document refer to UV7, UV5 and UV5Bio spectrophotometers running firmware version 2.0 or higher.
If you have any additional questions, contact your authorized METTLER TOLEDO dealer or service represen­tative.
u www.mt.com/contact
Conventions and symbols
Refers to an external document.
Note
for useful information about the product.
Elements of instructions
Prerequisites
§
1 Steps 2 ...
ð Intermediate results
ð Results
Introduction 3UV/VIS Excellence

2 Safety information

• Read and understand the information in this User Manual before you use the instrument.
• Keep this User Manual for future reference.
• Include this User Manual if you pass on the instrument to other parties.
If the instrument is not used according to the information in the Operating Instructions or if it is modified, the safety of the instrument may be impaired and Mettler-Toledo GmbH assumes no liability.
For a comprehensive description of the spectrophotometer and its functions, refer to the Operating Instructions.

2.1 Definition of signal words and warning symbols

Safety notes are marked with signal words and warning symbols. These show safety issues and warnings. Ignoring the safety notes may lead to personal injury, damage to the instrument, malfunctions and false results.
Signal words
WARNING
CAUTION
NOTICE
Warning symbols
Electrical shock Ultraviolet light beam Hot surface

2.2 Product specific safety notes

Intended use
This instrument is designed to be used in analytical laboratories by trained staff. The instrument is suitable for measuring molecular absorbance or transmittance in the ultra-violet (UV) and visible (VIS) range of analytical samples.
Any other type of use and operation beyond the limits of technical specifications without written consent from Mettler-Toledo GmbH is considered as not intended.
Responsibilities of the instrument owner
The instrument owner is the person that uses the instrument for commercial use or places the instrument at the disposal of the staff. The instrument owner is responsible for product safety and the safety of staff, users and third parties.
METTLER TOLEDO assume that the instrument owner provides the necessary protective gear, appropriate training for the daily work and for dealing with potential hazards in their laboratory.
Safety notes
for a hazardous situation with medium risk, possibly resulting in death or severe injury if not avoided.
for a hazardous situation with low risk, resulting in minor or moderate injury if not avoided.
for a hazardous situation with low risk, resulting in damage to the instrument, other material damage, malfunctions and erroneous results, or loss of data.
WARNING
Danger of death or serious injury due to electric shock!
Contact with parts that contain a live current can lead to injury and death. 1 Only use a METTLER TOLEDO power cable and AC adapter designed for your instrument. 2 Connect the power cable to a grounded power outlet. 3 Keep all electrical cables and connections away from liquids. 4 Replace damaged power cables and AC adapters immediately.
Safety information4 UV/VIS Excellence
CAUTION
Risk of eye damage from exposure to ultraviolet light beam
The light beam emitted from the UV/VIS instrument contains ultraviolet radiation and can cause eye damage.
− Never look directly into the light source.
NOTICE
Danger of damage to the instrument due to incorrect parts!
Using incorrect parts with the instrument can damage the Instrument or cause the instrument to malfunction.
− Only use parts supplied with the instrument, listed accessories and spare parts from METTLER TOLEDO.
Safety information 5UV/VIS Excellence

3 Design and Function

UV7
UV5
UV5 Bio
The UV/VIS excellence spectrophotometers based on an array setup. Array instruments have a robust mechanical design and do not contain any moving optical parts, improving the wavelength reproducibility. The array detector analyzes all wavelengths in parallel, measuring a complete spectrum very quickly.

3.1 Type definition and compatibility

UV7 UV5 UV5Bio
Main features
• FastTrack™ technology
• Excellence performance
• EUP and USP compliant
• Compact modularity
• Automation
• Direct measurements and
dedicated methods
• LabX® UV/VIS software

3.2 Overview

UV7, UV5, UV5Bio
Main features
• FastTrack™ technology
• Compact modularity
• Automation
• Direct measurements
• LabX® UV/VIS software
Main features
• FastTrack™ technology
• Powerful compactness
• Automation
• Direct Bio measurements and
specific methods
• LabX® UV/VIS software
Front and back covers
1
Type label
2
Housing
3
Power button
4
Terminal
5
LED instrument status (StatusLight™)
6
USB-connection for data transfer
7
Sample compartment
8
The StatusLight provides information about the status of the instrument.
Design and Function6 UV/VIS Excellence
StatusLight Instrument status
4
Green, steady light The instrument is ready for operation.
Green, blinking light The instrument is performing a task.
Orange, steady light The instrument waits for the user to perform an action.

3.2.1 1 cm Cuvette holder

A precision holder for positioning standard 1cm cuvettes.
Magnets
1
Alignment grooves
2
Base plate
3
Aperture for light channel
4
Cuvette clamping plate
5

3.3 Rear panel connections

RS232 standard port
1
Terminal port
3
2x USB A ports
5
(printer, flash drives, keyboard, mouse)
Mini-Din port (6-pin)
7
(FillPalMini)
Ethernet connection
2
1x USB B port
4
(connection to a PC with LabX™)
Power cable
6
(24V power supply)
Design and Function 7UV/VIS Excellence

3.4 User Interface

1
2
3
456
7
Task
Log out
Methods
User data Start
Home
Results
Setup
Direct
measurements
Tests &
Manual
Scan
Administrator
10/12/2015 11:45
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PumpScanBradford

3.4.1 Homescreen

Name Explanation
1 Shortcuts
2 Status bar
3 Instrument status
4 Start button
5 User data
6 Log out Logs out the current user. The menu Login is displayed after logging out.
User-specific shortcuts for frequently used methods are saved in this area. The shortcuts are saved in the user profile and can be defined, changed and deleted by the user.
The status bar contains the current menu item, user name as well as date and time.
A light strip indicates the current working status of the instrument.
Yellow
A method / direct measurement / performance test or manual operation is running.
Blue
No measurement is running.
Green
A method / direct measurement / performance test or manual operation is running but is waiting for user interaction.
Starts the method or direct measurement as was last run by the user. This does not apply to the manual operations or performance tests. This button is active only once a new user has started a method or a direct measurement for the first time.
Provides information about the user currently logged in .
Design and Function8 UV/VIS Excellence
Name Explanation
7 Menus Methods
Create, adapt and save measuring methods. This can be done for every measurement type.
Direct measurements
Measure a sample easily as a direct measurement. Direct measurements include the measurement types fixed wavelength, scan, quant and kinetics, as well as ready to use bio applications, such as DNA and protein concentration determination.
Results
Display, print or export measurement results. Here you can also access detailed information for all results.
Setup
Choose all system settings in this menu, e.g. hardware settings, user management or user preferences. These settings are usually defined during the installation of the instrument.
Tests & Manual
Entry point for editing and starting the performance tests and manual operations.
See also
2 Create and handle shortcuts}Page26

3.4.2 Menu structure

Methods
Methods has the following submenus:
• Fixed wavelength
• Scanning
• Bio applications (only UV5Bio)
• Quant
• Kinetics (only UV7 and UV5Bio)
Direct measurement
Direct measurement has the following submenus:
Fixed wavelength
Scanning
Bio applications (only UV5Bio) Protein
Quant
Kinetics (only UV7 and UV5Bio)
Results
Results has no submenus.
Setup
Setup has the following submenus:
–
–
Protein dye
Protein assay
Nucleic acid
Nucleic acid dye
Others
–
–
Design and Function 9UV/VIS Excellence
Quant calibrations
User settings Language
Auxiliary values Dyes & Values (only UV5Bio)
Hardware Automation
– –
Screen
Audio signal
StatusLight
Shortcuts
Keyboards
Auxiliary values
Dyes (only UV5Bio)
–
–
–
–
–
–
–
–
–
Peripherals Printer
Data export
Network settings
Network storage
PC settings
Barcode reader / Keyboard
Fingerprint reader
USB stick
CertiRef Information
Test sequence configuration
Monitoring (only UV7)
Performance test results
Performance test results
Performance test history
Auxiliary instrument
–
–
–
–
Global settings System Identification
Date/Time
Data storage
User management Users
Account policies
Analysis and resources behavior
Maintenance & Service MT-Service
Import / Export
Reset to factory settings
Firmware
Update
Hardware / Firmware summary
–
–
–
–
–
–
–
Tests & Manual
Tests & Manual has the following submenus:
• Performance test
• Automation
Design and Function10 UV/VIS Excellence
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