Mettler Toledo Refracto 30PX, Refracto 30GS Instruction Manual
Operating Instructions
Bedienungsanleitung
Mode d'emploi
Instrucciones de manejo
Istruzioni d'uso
Refracto 30PX
Refracto 30GS
EnglishDeutsch
Français
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Italiano
Contents
Contents Page
1 Introduction ...................................................................................3
2 Safety measures ............................................................................ 3
3 Description of the instrument ......................................................... 4
3.1 Refracto 30PX/GS ........................................................................... 4
3.2 Display .......................................................................................... 4
3.3 Keys ..............................................................................................5
4Tutorial.......................................................................................... 6
4.1 Inserting batteries ........................................................................... 6
4.2 Setting date and time ...................................................................... 6
4.3 Adjustment .....................................................................................6
4.4 Cleaning ........................................................................................ 6
4.5 Switching off and on ....................................................................... 7
5 Menu (menu) ................................................................................ 7
5.1 Using the menu .............................................................................. 7
5.2 Measurement Unit (Measure Unit) .................................................... 8
5.3 Temperature Unit (Temp. Unit) ....................................................... 10
5.4 Measure Mode .............................................................................. 10
5.5 Interface ....................................................................................... 10
5.6 Beep ............................................................................................ 11
5.7 Backlighting and LCD-Contrast (LCD)............................................. 11
5.8 Automatic Switch-off (Power) ........................................................ 11
5.9 Software Version (Version No.) ...................................................... 11
5.10 Date and Time (Date & Time) ........................................................ 11
5.11 Identification................................................................................. 11
6 Measurement (measure) ............................................................. 11
6.1 Procedure for proper measurement ................................................. 11
6.2 Pipetting the sample onto the measuring cell .................................. 12
6.3 Dipping the measuring cell into the sample..................................... 12
6.4 Measuring .................................................................................... 12
6.5 Saving the results ......................................................................... 13
6.6 Displaying and marking saved results ............................................ 13
6.7 Printing and transferring results...................................................... 13
6.8 Deleting results ............................................................................. 14
7 Adjustment (cal).......................................................................... 15
7.1 Adjusting the measuring cell with water .......................................... 15
7.2 Adjusting the measuring cell with air .............................................. 15
8 Interface...................................................................................... 16
8.1 METTLER TOLEDO LC-P45 printer settings....................................... 16
8.2 Data transfer to PC........................................................................ 16
8.3 Data format ..................................................................................17
9 Error messages and malfunctions ................................................ 18
10 Cleaning and maintenance .......................................................... 19
10.1 Cleaning the measuring cell........................................................... 19
10.2 Cleaning the housing .................................................................... 19
11 Standard and optional equipment................................................. 20
11.1 Standard equipment ......................................................................20
11.2 Optional equipment ....................................................................... 20
12 Technical data ............................................................................. 21
METTLER TOLEDO Refracto 30PX/GS 1
Contents
13 Appendix ..................................................................................... 23
13.1 Refractive index of pure water (15…40 °C) .................................... 23
13.2 Brix% .......................................................................................... 23
13.3 Brix%-table ..................................................................................24
13.4 HFCS42 and HFCS55 (inverted sugars).......................................... 25
13.5 HFCS42 table (0...76 Solids%) ..................................................... 26
13.6 HFCS55 table (0...80 Solids%) ..................................................... 27
Index ........................................................................................... 28
2
METTLER TOLEDO Refracto 30PX/GS
Introduction / Safety measures
1 Introduction
The METTLER TOLEDO Refracto 30PX and 30GS are portable measuring devices
suited for determining the refractive index of liquids. These devices determine the
refractive index by measuring the critical angle of total reflection of a light beam
falling on the sample. To carry out measurements, the sample is either pipetted
onto the measuring cell or the measuring cell is immersed directly in the sample.
The two Refracto versions are equipped with different measuring cells. The Refracto 30PX has a measuring cell made of optical glass, whereas the Refracto
30GS has a measuring cell made of sapphire. Sapphire has a higher refractive
index and a better thermal conductivity than glass. For this reason the Refracto
30GS has an extended measuring range (nD max. = 1.65) compared to the
Refracto 30PX (nD max. = 1.50) and registers the sample temperature more
quickly.
The results are automatically calculated into one of the following units: Refractive
index, Brix%, HFCS42, HFCS55, °Baumé, °Oechsle (CH,D), °KMW (Babo), T.A.
1990, % weight, % volume, specific gravity and freezing point (in °C or °F) of
sodium chloride solutions and ethanol/water mixtures, % weight, % volume and
freezing point (in °C and °F) of ethylene glycol and propylene glycol/water mixtures, % weight and % volume of isopropanol/water mixtures or a user-defined
unit. The value is then shown on the backlit display.
For exact measurements, it is imperative to correct the temperature’s influence on
the refractive index. The Refracto registers the sample temperature and automatically corrects the result. For the correction it uses either internally-stored tables or
one of the 10 temperature-compensation coefficients entered by the user.
The results, along with the sample identification, temperature, temperature-compensation coefficient, date and time, can be saved to the device. They can then,
together with the instrument identification, be transferred via the integrated infrared interface to a computer or to a printer.
2 Safety measures
Measures for your protection
- Do not work in an explosion-hazardous environment! The instrument housing is not gastight. Otherwise, there is a risk of
explosion from sparks and/or risk of corrosion by gasses which
can seep in.
Measures for operational safety
- When doing dip-in measurements, never immerse the instrument
deeper into the sample than the indicated line. The instrument is
only resistant to splashed water.
- Use batteries of the specified type only. Otherwise, proper operation cannot be guaranteed.
- Ensure that the following environmental conditions are met:
• no strong vibrations present
• not in direct sunlight
• no high humidity present
• no corrosive gasses present
• temperature between – 20 °C and 70 °C
• no strong electrical or magnetic fields present
METTLER TOLEDO Refracto 30PX/GS 3
Description of the instrument
3 Description of the instrument
3.1 Refracto 30PX/GS
Illustration, see rear fold-out page
1 Backlit display
2 Keypad
3 Measuring cell
4 Prism
5 Infrared interface
6 Battery compartment cover
3.2 Display
Illustration, see rear fold-out page
1 Selected unit of measurement
2 Result
3 Sample identification (A…Z or space)
4 Sample number, or error number if an error has occurred
5 Appears if Memory in is set to Auto
6 Appears if Memory out is set to Auto. If a printer or PC is
connected, the data are transferred automatically
7 Battery-power indicator
8Temperature (°C / °F)
9 Appears if delete mode is activated
10 Mark for results
For the identification of invalid or incorrect results or for marking a
change in the sample series
4
METTLER TOLEDO Refracto 30PX/GS
Description of the instrument
3.3 Keys
Illustration, see front fold-out page
Red symbols: Press key longer than 2 seconds.
Blue symbols: Press key briefly.
No. Symbol Short keys press Long key press
1 ok/ • Start measurement
measure • Confirm input
• Confirm data delete
• Confirm data transfer
• While pressing and holding
key 2: enter the menu
2 esc • Exit the menu •
3 • Move marker to the right • Transfer a series of saved
• Display saved results results to a printer/PC
• Transfer one saved result to
a printer/PC
4 • Move marker downward • Call up adjustment mode
• Select sample number
(descends)
• Switch between Yes and
No setting
5 • Move marker to the left • Delete saved results
• Mark saved results
6 • Move marker upward • Select temperature-com-
• Select sample number pensation coefficient α
(ascends)
• Switch between Yes and
No setting
Only the arrow symbols are used to represent keys 3 – 6 in the following
operating instructions.
Switch instrument on or off
METTLER TOLEDO Refracto 30PX/GS 5
Tutorial
4Tutorial
4.1 Inserting batteries
- Open the battery compartment cover on the backside of the Refracto by turning
it counterclockwise with a coin.
- Insert batteries into the battery compartment, observing correct polarity.
- Close the battery compartment cover by turning it clockwise with a coin.
The instrument switches on automatically and is immediately ready for operation.
The battery lifetime is approximately 60 hours with the backlighting turned off (at
1 measurement per minute). If nothing appears in the display: Check polarity of
the batteries.
4.2 Setting date and time
- Press ok/measure and esc simultaneously to enter the menu.
-Press repeatedly until Date & Time appears in reverse display.
- Confirm
- Confirm
- Use the and keys to choose the number to be changed, then use the
- Use the key to switch to Time and confirm with ok/measure. Set the time
- Press esc to leave the menu.
Date & Time
Date
with the ok/measure key to change the date.
or keys to change it. Confirm with ok/measure.
as described above.
with the ok/measure key.
4.3 Adjustment
- Put the instrument on a table.
- Pipette some drops of water onto the measuring cell. Press and hold the
key until
automatically (duration: several seconds). After adjustment is completed, the
measured deviation from the theoretical value and Execute ? (No) appears.
- If the measured deviation is > 0.0005: Dry the measuring cell and clean it
with a cleaning tissue. Press the ok/measure key and repeat the step above.
-Press the or key. Execute? (
-Press the ok/measure key.
The adjustment is confirmed.
CALIB (Water)
appears in the display. The instrument adjusts
) appears.
Yes
4.4 Cleaning
Remains of the sample on the prism reduce the accuracy of the Refracto measurements. The Refracto therefore must be cleaned carefully after each measurement.
- Clean the measuring cell with a cleaning tissue to remove remains of the
sample completely.
6
METTLER TOLEDO Refracto 30PX/GS
Tutorial / Menu (menu)
4.5 Switching off and on
Switching off
- Press and hold the esc key until the display is turned off.
The instrument is now turned off.
Switching on
- Press and hold the esc key until the display appears.
The instrument is ready for operation.
5 Menu (menu)
The menu of the Refracto offers the following functions:
• Measurement Unit (Measure Unit) see Chapter 5.2
• Temperature Unit (Temp. Unit) see Chapter 5.3
• Measure Mode see Chapter 5.4
• Interface see Chapter 5.5
• Beep see Chapter 5.6
• Backlighting and LCD-Contrast (LCD) see Chapter 5.7
• Automatic Switch-off (Power ) see Chapter 5.8
• Software Version (Version No.) see Chapter 5.9
• Date and Time (Date & Time) see Chapter 5.10
• Identification see Chapter 5.11
5.1 Using the menu
Entering the menu
-Press the keys ok/measure and esc simultaneously.
The instrument switches to the menu.
Selecting functions
- Press the and keys repeatedly until the desired function is marked.
- Press the ok/measure key to activate the marked function.
The instrument switches to the corresponding submenu or activates the desired
function.
Numerical input
- Select decimal places with the and keys.
- Change value with the and keys.
- Press the ok/measure key to confirm the value.
Exiting the menu
-Press the esc key.
METTLER TOLEDO Refracto 30PX/GS 7
Menu (menu)
5.2 Measurement Unit (Measure Unit)
The measurement units of the Refracto are grouped according to their field of
application:
- nD, nDt: General applications
- Sugar: Sugar determinations
- Conc.: General concentration determinations
- Alcohol: Alcohol (Ethanol)
-IPA: Isopropanol
- Salinity: Salt (sodium chloride)
- Wine: Determination of the sugar content in grape juice
- Antifreeze: Antifreeze
nD (refractive index)
Measurement of the refractive index (nD).
nDt (temperature-compensated refractive index)
T
Refractive index (n
All results are calculated for the same reference temperature (T0, e.g. 20 °C),
0
) measurement at a reference temperature.
D
regardless of the measuring temperature (T). Measuring and reference temperature have to be in the same unit (°C or °F).
Compensated refractive index = measured refractive index + α • (T – T0)
10 temperature-compensation coefficients can be stored in the instrument.
The following input is required:
Comp. No. Number of the temperature compensation coefficient (0…9)
Comp. Temp. Reference temperature ( T0)
α x 1000: Temperature-compensation coefficient.
For calculation of α, see page 9.
Sugar (sugar determinations)
Display of the results as Brix% (sucrose), HFCS42 or HFCS55 (high fructose corn syrup). See appendix.
Conc. (concentration determinations)
Measurement of the concentration via the input of the desired concentrationconversion formula y = a + bx at a reference temperature.
y= Concentration in % or without units
a, b = sample dependent coefficients.
x= measured refractive index
Input of the temperature-compensation coefficient see nDt.
8
METTLER TOLEDO Refracto 30PX/GS
Menu (menu)
Alcohol (alcohol determinations)
Measurements of ethanol/water mixtures. Display of the results in one of the
following units: % weight (wt%), % volume (vol%) of ethanol at 20 °C, specific gravity (SG) or freezing point (FP) of the mixture (in °C or °F). Selection of
the temperature unit for the freezing point via Temp. Unit (see chapter 5.3).
Range of measurement: 0.0…50.0 % weight (0.0…67.7 % volume)
IPA (isopropanol determinations)
Measurements of isopropanol/water mixtures. Display of the results in one of the
following units: % weight (wt%) or % volume of isopropanol (vol%) at 20 °C.
Range of measurement: 0.0 … 40.0 % weight (0.0 … 47.4 % volume)
Salinity (salt concentration determinations)
Measurements of sodium chloride/water mixtures. Display of the results in one of
the following units: % weight NaCl (%NaCl), specific gravity (SG) or freezing
point (FP) of the solution (in °C or °F). Selection of the temperature unit for the
freezing point via Temp. Unit (see chapter 5.3).
Wine (determination of the sugar content in grape juice)
Display of the result in one of the following units: “titre alcoométrique” %vol 1990 (T.A(90)), Swiss °Oechsle (Oechsle), German °Oechsle (Oechsle (D)), degrees “Klosterneuburger Mostwaage” (KMW(babo)) or degrees Baumé (Baume).
Antifreeze
Measurements of ethylene glycol/water- and propylene glycol/water-mixtures.
Display of the results in one of the following units: % weight ethylene glycol or
propylene glycol (wt% EG, wt% PG), % volume ethylene glycol or propylene
glycol (v% EG, v% PG) at 20°C or freezing point (FP EG, FP PG) of the
mixture (in °C or °F). Selection of the temperature unit for the freezing point via
Temp. Unit (see chapter 5.3).
Range of measurement:
Ethylene glycol: 0.0…60.0 % weight (0.0… 58.2 % volume)
Propylene glycol: 0.0…55.0 % weight (0.0… 55.2 % volume)
Calculation of the temperature-compensation coefficient α
- Measure the refractive index (nD) of the sample
• at a temperature (T1) below the normal measuring temperature (n
• at a temperature (T2) above the normal measuring temperature (n
- Calculate α according to the formula:
T
T
2
1
n
– n
D
D
T – T
2
1
T
1
)
D
T
2
)
D
- Enter value α x 1000 into the instrument.
Note
The temperatures T1 and T2 have to be entered in the selected unit (°C or °F, see
chapter 5.3).
METTLER TOLEDO Refracto 30PX/GS 9
Menu (menu)
Example
(measured) refractive index at 15°C (T1): 1.3334
(measured) refractive index at 26°C (T2): 1.3324
1.3334 – 1.3324
26 – 15
α x 1000 = 0,091; enter this value into the instrument.
= 0.0000909
5.3 Temperature Unit (Temp. Unit)
Unit for displaying the temperature in °C or °F (selectable).
5.4 Measure Mode
Configuration of sample identification and method of data storage.
Sample Name Sample identification.
Mode Method of data storage.
A letter (A…Z or space) can be set for the identification of
samples.
Labo By pressing the ok/measure key the result is saved and trans-
ferred (printer, PC).
Field By pressing the ok/measure key the result is saved. Custom User-defined setting.
Memory in Save results.
Auto Save result automatically.
Manu Result saved by pressing the
ok/measure key.
Memory out Transfer result to PC or printer.
Auto Transfer result automatically.
Manu Transfer result by pressing the
key.
5.5 Interface
PRN Printer interface
Data transfer to the printer
Printer with serial interface and connected infrared adapter. Results are
formatted for output to a printer.
Transfer rate (baud rate), parity, stop and data bits must be configured
according to the peripheral device.
The following settings are required for the METTLER TOLEDO LC-P45
printer:
Baud rate 9600
Parity none
Stop bits 1
Data bits 8
RS Serial interface. The Excel macro “PortableCapt” for data transfer to the
PC via the infrared adapter is located on the HelloCD™.
See Chapter 8.2.
IrDA Data transfer to the PC with the integrated IrDA interface in accordance
with protocol 1.20.
10
METTLER TOLEDO Refracto 30PX/GS
Menu (menu) / Measurement (measure)
5.6 Beep
Off Beep off.
On Beep on.
5.7 Backlighting and LCD-Contrast (LCD)
Backlighting
The backlighting is turned off automatically 5 seconds after the last time a key
has been pressed (Auto off), or it is always off (Always off).
Contrast
Display contrast is adjustable to one of 9 levels with the keys.
5.8 Automatic Switch-off (Power)
Off Automatic switch-off off. The instrument must be switched off manually.
On
The instrument switches off automatically if not operated for 10 minutes.
5.9 Software Version (Version No.)
The software version is displayed.
5.10 Date and Time (Date & Time)
The settings for date and time can be edited here. The date is displayed in the
format year/month/day (e.g. 2003/03/04 for March 4, 2003). Both date and
time are included in the data transfer to a printer or computer.
5.11 Identification
An identification consisting of 10 letters and numbers (instrument name, user,
etc.) may be entered here. This identification will be included in the data transfer
to a printer or computer.
6 Measurement (measure)
6.1 Procedure for proper measurement
- Ensure that the prism and the measuring cell are clean before each measurement. Insufficient cleaning leads to remains on the prism and, therefore, incorrect results.
- Check the sample for chemical resistance of the following materials:
• Prism: Glass (Refracto 30GS: sapphire)
• Measuring cell: Stainless steel SUS 316 (Refracto 30GS: gold)
• Housing: PBT (polyester)
- Ensure that the samples to be measured
• have reached more or less ambient temperature
• are homogeneous. Viscous and highly concentrated liquids have to be mixed
thoroughly before a sample is taken or a measurement is carried out.
• can be dissolved with a solvent suitable for cleaning the measuring cell.
- Adjust the instrument at regular intervals (see chapter 7).
METTLER TOLEDO Refracto 30PX/GS 11
Measurement (measure)
6.2 Pipetting the sample onto the measuring cell
- Pipette the sample onto the measuring cell. The measuring cell has to be filled
up to the mark.
6.3 Dipping the measuring cell into the sample
X
- Immerse the measuring cell completely in the sample.
- Caution: Never immerse the instrument deeper into the sample than the indicated line. The instrument is only resistant to splashed water.
6.4 Measuring
The procedure is dependent upon the settings in the menu, see chapter 5.4
Measuring without temperature-compensation coefficients (α):
- Press the ok/measure key to start the measurement.
Measuring with temperature-compensation coefficients (α) (nDt or Conc. on
the display):
- Press the key until a previously saved temperature-compensation coefficient appears in the display, e.g. .
- Select the desired coefficient using the and keys.
- Confirm the selected coefficient with the ok/measure key.
- Press the ok/measure key to start the measurement.
12
METTLER TOLEDO Refracto 30PX/GS
Measurement (measure)
6.5 Saving the results
The instrument can save up to 1100 results internally. Each time a result is
saved, the sample number increases by one.
The symbol appears in the display: Saving all results automatically
The instrument saves all results automatically. After the measurements, as soon
as the result is saved, Ready appears on the display.
The symbol does not appear in the display: Saving selected results manually
After the measurements, the result appears in reverse display.
Save the result:
-Press the ok/measure key.
Do not save the result:
-Press the esc key.
Ready appears on the display and the instrument is ready for the next measure-
ment.
6.6 Displaying and marking saved results
Displaying saved results
-Press the key.
The sample number flashes and the symbol appears.
- Scroll through the saved results using the and keys.
Marking results
For the identification of invalid or incorrect results or for marking a sample change.
- Select the desired sample number using the and keys.
-Press the ok/measure key.
The selected sample number is marked with an asterisk.
Note
The marking is removed if the sample has already been marked.
6.7 Printing and transferring results
Conditions
• The interface and peripheral device are configured properly, see Chapter 5.5
and Chapter 8.
• For PRN and RS interfaces, the infrared adapter must be connected to the
printer/PC.
The procedure for printing and transferring results is dependent upon the settings
in the menu (see Chapter 5.4).
Important
To transfer or print results, hold the instrument in the direction of the infrared
adapter at a maximum distance of approx. 20 cm.
The symbol appears in the display: Printing or transferring results
automatically
Every displayed result is transferred automatically.
METTLER TOLEDO Refracto 30PX/GS 13
Measurement (measure)
The symbol does not appear in the display: Printing or transferring selected
results manually
-Press the key.
- Select desired result with the or key.
- Press the ok/measure key to transfer/print the result.
The symbol flashes and the result is transferred.
Printing and transferring results of a series of samples manually
- Press and hold the key until
pears in the display.
-Press the or key.
Execute? (
- Press the ok/measure key to confirm.
- Enter the desired series of samples (from ... to ...) using the arrow keys.
To transfer the series of samples:
-Press the ok/measure key.
The sample series (from ... to ...) is confirmed. The symbol flashes and the
results of the selected series of samples are transferred.
To print or transfer all results manually
- Press and hold the key until
pears in the display.
-Press the ok/measure key.
The symbol in the display flashes and all results are transferred.
After a successful transfer, the user is asked whether he/she would like to delete
the transferred results:
Leaving transferred results intact
- Confirm
sure button.
Deleting transferred results
-Press the or key.
Execute? (
- Press the ok/measure key to confirm.
All results are deleted.
Range
) appears.
Memory All Clear
Memory All Clear
) appears.
Yes
Memory out
Memory out
Execute? (No) by pressing the ok/mea-
, Execute? (
, Execute? (
Execute? (No).
All
All
) ap-
) ap-
6.8 Deleting results
It is not possible to delete individual results with the Refracto.
Deleting all results
- Press and hold the key until
appears in the display.
-Press the or key.
Execute? (
- Press the ok/measure key to confirm.
All results are deleted.
14
Yes
) appears.
Memory All Clear
METTLER TOLEDO Refracto 30PX/GS
Execute? (No)
Adjustment (cal)
7 Adjustment (cal)
7.1 Adjusting the measuring cell with water
- Ensure that the measuring cell and the surface of the prism are clean.
-Transfer pure distilled water onto the measuring cell by means of the pipette
which comes with the instrument. The measuring cell has to be filled up to the
mark.
-Wait until the water has reached more or less ambient temperature.
-Press and hold the key, until
The instrument adjusts automatically (duration: several seconds). After adjustment is completed, the measured deviation from the theoretical value and
Execute ? (No) appears.
Measured deviation < 0.0005
-Press the or key.
- Execute ? (
-Press the ok/measure key.
The adjustment is confirmed.
Measured deviation ≥ 0.0005
- Check whether the measuring cell and the surface of the prism are clean.
Measuring cell and the surface of the prism are clean:
-Press the or key.
- Execute? (
-Press the ok/measure key.
Measuring cell and/or the surface of the prism are dirty:
- Confirm Execute ? (No) by pressing the ok/measure key.
- Clean the cell and the surface of the prism and repeat the adjustment.
Yes
) appears.
Yes
) appears.
CALIB (Water)
appears in the display.
7.2 Adjusting the measuring cell with air
The Refracto measures with the specified accuracy if the measuring cell is correctly adjusted with distilled water. An adjustment with air is only necessary if the
error message E-01 appears frequently during measurements or adjustments.
Preparing the measuring cell
- Clean the measuring cell and the surface of the prism thoroughly with a cleaning tissue. Wait until the surface of the prism is completely dry.
Adjusting
-Press the and keys simultaneously.
CALIB (Air)
cell automatically.
Adjustment is complete when
- Adjust measuring cell with distilled water (see chapter 7.1)
If the error message E-01 appears in the display during the adjustment with air,
call METTLER TOLEDO service.
METTLER TOLEDO Refracto 30PX/GS 15
appears in the display. The instrument adjusts the measuring
CALIB (Air)
disappears.
Interface
8 Interface
Using the infrared interface of the Refracto, the stored measurements together
with the sample identification, measurement unit, temperature, temperature-compensation coefficient, instrument identification, date and time can be printed out
with a printer or transferred to a PC.
An infrared adapter or a PC/printer with an IrDA interface is required.
Important
Data transfer is only possible when:
• there is visual contact between the infrared adapter and the infrared interface;
• the distance between the Refracto and the infrared adapter is less than 20 cm.
8.1 METTLER TOLEDO LC-P45 printer settings
- Configure the infrared interface of the Refracto as described in Chapter 5.5.
- Connect infrared adapter to printer.
- Switch printer on.
-Press the menu button on the printer.
- Set the following serial interface parameters, see the operating instructions for
the printer:
Baud rate: 9600
Parity: None
Stop bits: 1
Data bits: 8
8.2 Data transfer to PC
- Configure the infrared interface of the Refracto as follows (Interface, see chapter 5.5):
Interface: RS
Baud rate: 9600
Parity: None
Stop Bits: 1
Data Bits: 8
- Connect the infrared adapter to an available serial interface (COM1, COM2,
etc.) on the PC.
- Insert the accompanying HelloCD™ into the CD-ROM drive of the PC.
- Install the PortableCapt program (Excel macro).
- Start the PortableCapt program on the PC.
- In the Excel macro: select the serial interface (COM1, COM2, ...) to which the
infrared adapter is connected.
- For further procedures see Chapter 6.7.
16
METTLER TOLEDO Refracto 30PX/GS
Interface
8.3 Data format
If RS is selected as interface, the data is transferred in the following format:
1)
Sample identification (A…Z, space)
2)
If result is marked (*); otherwise, space
3)
Format yyyy/mm/dd hh:mm
4)
Temperature unit (°C or °F)
5)
Number of the temperature-compensation coefficient
6)
These contain only spaces, unless nDt or Conc. is selected as the result unit
METTLER TOLEDO Refracto 30PX/GS 17
Error messages and malfunctions
9 Error messages and malfunctions
Error Possible causes What to do
E-01 Intensity of the light source not - Carry out an adjustment
correctly adjusted. with air
E-02 Error during adjustment with air - Clean the surface of the prism
• The surface of the prism is dirty and repeat the adjustment with
E-03 Error during adjustment with water - Put water on the measuring
• There was no water on the cell and repeat adjustment
measuring cell during the - If the error message appears
adjustment again: Check whether the light
E-05 Full appears instead of a - Delete data from memory
sample number:
• Data memory full
E-06 Memory fault - Call METTLER TOLEDO Service
E-07 Measuring time of 3 minutes - Switch instrument off and
exceeded on again
BATT Batteries empty - Replace batteries
No There is no sample on the - Put a sample on the measuring
Samp. measuring cell cell and repeat measurement
No Error during measurement - Wait until the sample has
Meas. This error normally shows up if reached ambient temperature
the difference between sample and repeat measurement
and ambient temperature is too
high
Range The refractive index of the sample - Measure only samples with a
Over is not within the range of meas- refractive index in the range of
urement of the Refracto 1.32…1.50 (Refracto 30PX)
- If the error message appears
again: Check whether the light
source works and call METTLER
TOLEDO Service
air
source works and call METTLER
TOLEDO Service
- Carry out a measurement with
pure distilled water. If error
appears again:
- Call METTLER TOLEDO Service
(see Chapter 4.1)
- Measure only samples with a
refractive index in the range of
1.32...1.65 (Refracto 30GS)
18
METTLER TOLEDO Refracto 30PX/GS
Cleaning and maintenance
10 Cleaning and maintenance
10.1 Cleaning the measuring cell
Remains of the sample on the prism reduce the accuracy of the
Refracto measurements. The Refracto therefore must be cleaned carefully after
each measurement.
- Rinse and dry the end of the instrument after dip-in measurements.
- Clean the measuring cell with a cleaning tissue to remove remains of the
sample completely.
- Never use aggressive liquids or solvents to clean the housing of the Refracto.
- Be careful never to scratch the surface of the prism when cleaning it!
-We recommend that you use the cleaning tissues which come with the instrument.
10.2 Cleaning the housing
- Never use aggressive liquids or solvents to clean the housing of the Refracto.
-We recommend that you use the cleaning tissues which come with the
instrument.
METTLER TOLEDO Refracto 30PX/GS 19
Standard and optional equipment
11 Standard and optional equipment
Each part identified by an order number can be ordered from METTLER TOLEDO.
11.1 Standard equipment
The instrument is delivered pre-assembled.
Order No.
1 Refracto 30PX refractometer Refracto 30PX
with carrying case,
or
Refracto 30GS refractometer Refracto 30GS
with carrying case.
Includes:
2 AAA-type batteries (LR03, 1.5 V)
5 Cleaning tissues
2 Pipettes (LD-PE)
2 Vials with screw cap (PE)
1 HelloCD™ (CD-ROM) 51325001
1 Operating instructions 51710074
Operating Instructions
Bedienungsanleitung
Mode d'emploi
Manual de Instrucciones
Istruzioni d'uso
Refracto 30P
11.2 Optional equipment
Order No.
Cleaning tissues, 10 pcs. 51325003
Battery compartment cover 51324708
Memo cover, 10 pcs. 51324700
Memo paper, 10 pcs. 51324701
Infrared adapter 51325006
Printer LC-P45
20
METTLER TOLEDO Refracto 30PX/GS
12 Technical data
Technical data
Measurement principle Measurement of the refractive index by
Light source LED, λ = 589.3 nm
Sampling By means of a pipette (bench-top mode)
Working temperature 10...40 °C
Storage temperature –20...70 °C
Temperature accuracy ±0.2 °C
Range of measurement nD
(refractive index) 1.32...1.50 (Refracto 30PX)
Range of measurement nD
(refractive index) 1.32...1.65 (Refracto 30GS)
Accuracy nD (refractive index) ±0.0005
Resolution 0.0001
Range of measurement Brix 0...85 %
Accuracy ±0.2 %
Resolution 0.1 %
Display Backlit LC-Display
Materials
Housing PBT (Polyester)
Measuring cell (Refracto 30PX) Stainless steel SUS 316, glass
Measuring cell (Refracto 30GS) hard gold-plated brass, sapphire
Materials in contact with sample
Materials in contact with sample PBT, gold, sapphire (Refracto 30GS)
Weight approx. 200 g
Measuring time per sample 3...180 seconds
Data memory 1100 results
Interface Infrared for printer and PC
Battery operation 2 x 1.5 V batteries (LR03); type AAA
Battery lifetime approx. 60 hours (at 1 measurement per
determination of the critical angle of total
reflection.
or by dipping the measuring cell into the
sample (dip-in mode)
PBT, stainless steel, glass (Refracto 30PX)
minute and backlighting off)
Range of measurements and accuracy of remaining units: see next page.
Subject to technical changes.
METTLER TOLEDO Refracto 30PX/GS 21
Technical data
Unit Range of measurement Accuracy
HFCS42 [%] 0.0...75.0 ±0.2 0.1
HFCS55 [%] 0.0...80.0 ±0.2 0.1
Alcohol [Wt%] 0.0...20.0 ± 0.6 0.1
20.0...50.0 ±2.0 0.1
Alcohol [Vol%] 0.0...24.5 ± 0.6 0.1
24.5...67.7 ±2.0 0.1
Alcohol SG 0.915...1.000 ±0.004 0.001
Alcohol FP [°C] 0.0...–30.0 ± 1.0 0.1
IPA [Wt%] 0.0...20.0 ±0.6 0.1
20.0...40.0 ±2.0 0.1
IPA [Vol% ] 0.0...24.7 ± 0.6 0.1
24.7...47.4 ±2.0 0.1
NaCl [Wt%] 0.0...26.0 ±0.4 0.1
NaCl SG 1.000...1.199 ±0.003 0.001
NaCl FP [°C] 0.0...–27.0 ± 1.0 0.1
EG [Wt%] 0.0...60.0 ±0.6 0.1
EG [Vol%] 0.0...58.2 ±0.6 0.1
EG FP [°C] 0.0...–50.0 ±1.0 0.1
PG [Wt%] 0.0...55.0 ± 0.6 0.1
PG [Vol%] 0.0...55.2 ± 0.6 0.1
PG FP [°C] 0.0...–35.0 ± 1.0 0.1
Wine [TA(90)] 5.0...38.0 ± 0.1 0.1
Wine [°Oe] 0.0...260.0 ± 1.0 0.1
Wine [°Oe(D)] 0.0...260.0 ±1.0 0.1
Wine [KMW (babo)] 0.0...45.0 ±0.2 0.1
Wine [°Baumé] 0.0...29.0 ±0.2 0.1
Resolution
22
METTLER TOLEDO Refracto 30PX/GS
Appendix
13 Appendix
13.1 Refractive index of pure water (15…40 °C)
Temp Temp Temp
[°C] n
D
15 1.3334 25 1.3325 35 1.3312
16 1.3333 26 1.3324 36 1.3311
17 1.3332 27 1.3323 37 1.3310
18 1.3332 28 1.3322 38 1.3308
19 1.3331 29 1.3321 39 1.3307
20 1.3330 30 1.3319 40 1.3305
21 1.3329 31 1.3318
22 1.3328 32 1.3317
23 1.3327 33 1.3315
24 1.3326 34 1.3314
[“Handbook of Chemistry and Physics, 56th Edition”]. Values divided by the
refractive index of air at the corresponding temperatures, according to the formula given in [“Handbook of Chemistry and Physics, 75th Edition”].
13.2 Brix%
[°C] n
D
[°C] n
D
Brix% represents the concentration in weight percent of a mixture of pure sucrose
and water (g sucrose per 100 g solution) and is used to express the % weight
concentration of sucrose solutions in general. Brix% is calculated from the measured refractive index using a concentration table stored in the instrument (source:
20th conference of the International Commission of Uniform Methods for Sugar
Analysis ICUMSA). The results are given at the standard reference temperature of
20 °C using the temperature dependence for these solutions from the same source.
The refractive index and the prism surface temperature are thus measured and
the Brix% is calculated from the tables.
Note: When substances other than sucrose are present in the sample the
result in Brix% will not indicate the real sucrose concentration.
METTLER TOLEDO Refracto 30PX/GS 23
Appendix
13.3 Brix%-table
[20th session ICUMSA, Colorado Springs 1990]
Brix% n
20
D
Brix% n
0.0 1.33299 30.0 1.38115 60.0 1.44193
1.0 1.33442 31.0 1.38296 61.0 1.44420
2.0 1.33586 32.0 1.38478 62.0 1.44650
3.0 1.33732 33.0 1.38661 63.0 1.44881
4.0 1.33879 34.0 1.38846 64.0 1.45113
5.0 1.34026 35.0 1.39032 65.0 1.45348
6.0 1.34175 36.0 1.39220 66.0 1.45584
7.0 1.34325 37.0 1.39409 67.0 1.45822
8.0 1.34476 38.0 1.39600 68.0 1.46061
9.0 1.34629 39.0 1.39792 69.0 1.46303
10.0 1.34782 40.0 1.39986 70.0 1.46546
11.0 1.34937 41.0 1.40181 71.0 1.46790
12.0 1.35093 42.0 1.40378 72.0 1.47037
13.0 1.35250 43.0 1.40576 73.0 1.47285
14.0 1.35408 44.0 1.40776 74.0 1.47535
15.0 1.35568 45.0 1.40978 75.0 1.47787
16.0 1.35729 46.0 1.41181 76.0 1.48040
17.0 1.35891 47.0 1.41385 77.0 1.48295
18.0 1.36054 48.0 1.41592 78.0 1.48552
19.0 1.36218 49.0 1.41799 79.0 1.48810
20.0 1.36384 50.0 1.42009 80.0 1.49071
21.0 1.36551 51.0 1.42220 81.0 1.49333
22.0 1.36720 52.0 1.42432 82.0 1.49597
23.0 1.36889 53.0 1.42647 83.0 1.49862
24.0 1.37060 54.0 1.42862 84.0 1.50129
25.0 1.37233 55.0 1.43080 85.0 1.50398
26.0 1.37406 56.0 1.43299
27.0 1.37582 57.0 1.43520
28.0 1.37758 58.0 1.43743
29.0 1.37936 59.0 1.43967
20
D
Brix% n
20
D
24
METTLER TOLEDO Refracto 30PX/GS
Appendix
13.4 HFCS42 and HFCS55 (inverted sugars)
HFCS stands for High-Fructose Corn Syrup. These syrups are manufactured from
natural corn syrup and consist of inverted (or isomerized) sugar, i.e. a mixture of
dextrose, fructose, maltose and sucrose. They are classified according to their
fructose content. Mainly three different HFCS are in use: a mixture containing
42 % fructose (HFC2S42), one containing 55 % fructose (HFCS55) and a third
one containing 90 % fructose (HFCS90).
Inverted sugar concentration denominates the concentration in weight % of an
isomerized sugar solution. Inverted sugar concentration can be converted from
the refractive index of the inverted sugar solution measured at 20 °C (n
Refracto has stored conversion tables (“Physical Properties Table”, Corn Products, 1991) for two of the mixtures (HFCS42 and HFCS55) as well as a table for
temperature compensation.
The relation between inverted sugar concentration and refractive index varies
with the composition of the inverted sugar. Compositions of the inverted sugar
syrups HFCS42 and HFCS55 are shown below.
HFCS42 HFCS55
Fructose 42.50 % 55.40 %
Dextrose 52.50 % 40.30 %
Maltose 3.00 % 3.00 %
Sucrose 0.00 % 0.00 %
Maltotriose DP3 0.70 % 0.40 %
Oligosaccharide DP4 1.30 % 0.90 %
Sulfated Ash 0.03 % 0.05 %
20
D
). The
METTLER TOLEDO Refracto 30PX/GS 25
Appendix
13.5 HFCS42 table (0...76 Solids%)
[Physical Properties Table: Corn Products, 1991]
HFCS42 HFCS42 HFCS42
Solids% n
20
Brix% Solids% n
D
20
Brix% Solids% n
D
20
Brix%
D
0.0 1.33299 0.00 30.0 1.38059 29.71 60.0 1.43949 58.95
1.0 1.33441 1.00 31.0 1.38236 30.69 61.0 1.44168 59.91
2.0 1.33585 1.98 32.0 1.38414 31.67 62.0 1.44387 60.88
3.0 1.33729 2.98 33.0 1.38594 32.65 63.0 1.44608 61.84
4.0 1.33874 3.97 34.0 1.38775 33.64 64.0 1.44831 62.81
5.0 1.34021 4.97 35.0 1.38957 34.61 65.0 1.45055 63.78
6.0 1.34169 5.95 36.0 1.39141 35.60 66.0 1.45281 64.74
7.0 1.34318 6.95 37.0 1.39325 36.58 67.0 1.45508 65.71
8.0 1.34468 7.94 38.0 1.39511 37.55 68.0 1.45737 66.67
9.0 1.34619 8.93 39.0 1.39699 38.53 69.0 1.45968 67.64
10.0 1.34771 9.93 40.0 1.39887 39.51 70.0 1.46200 68.60
11.0 1.34924 10.92 41.0 1.40077 40.49 71.0 1.46433 69.56
12.0 1.35078 11.90 42.0 1.40269 41.47 72.0 1.46669 70.53
13.0 1.35234 12.90 43.0 1.40461 42.44 73.0 1.46905 71.49
14.0 1.35391 13.90 44.0 1.40655 43.42 74.0 1.47144 72.46
15.0 1.35549 14.89 45.0 1.40851 44.39 75.0 1.47384 73.42
16.0 1.35708 15.87 46.0 1.41048 45.36 76.0 1.47626 74.38
17.0 1.35868 16.87 47.0 1.41246 46.34
18.0 1.36029 17.86 48.0 1.41445 47.31
19.0 1.36192 18.85 49.0 1.41646 48.28
20.0 1.36355 19.84 50.0 1.41848 49.26
21.0 1.36520 20.83 51.0 1.42052 50.22
22.0 1.36686 21.81 52.0 1.42257 51.20
23.0 1.36854 22.80 53.0 1.42464 52.16
24.0 1.37022 23.79 54.0 1.42671 53.14
25.0 1.37192 24.78 55.0 1.42881 54.11
26.0 1.37363 25.76 56.0 1.43092 55.07
27.0 1.37535 26.75 57.0 1.43304 56.04
28.0 1.37708 27.74 58.0 1.43518 57.01
29.0 1.37883 28.72 59.0 1.43733 57.98
26
METTLER TOLEDO Refracto 30PX/GS
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