Mettler Toledo Rainin Classic Operating Instructions Manual

Operating Instructions

Rainin Classic

Manual Pipette

™

2

Continuously Adjustable Pipettes

Intent of Use

Rainin Classic air-displacement pipettes are intended for dispensing liquids in the volume
ranges from 0.1 µL to 10 mL in various in-vitro liquid handling applications.

Table of Contents

Description ........................................................................................................................... 3

Autoclaving ........................................................................................................................... 3

Volume Indicator. .................................................................................................................. 3

Safety Filter, PR-5000, PR-10ML ............................................................................................. 4

Tip Selection ......................................................................................................................... 4

Tip Immersion Depth .............................................................................................................. 4

Tip Angle .............................................................................................................................. 5

Operation ............................................................................................................................. 5

Pipetting Guidelines & Precautions .......................................................................................... 6

Pre-Rinsing Recommended .................................................................................................... 6

Reverse Mode Pipetting .......................................................................................................... 6

Pipetting Liquids of Varying Density ......................................................................................... 7

Temperature Considerations .................................................................................................... 7

Acids and Corrosives ............................................................................................................. 7

Storage ................................................................................................................................ 7

Removing/Replacing Ejector Arm ............................................................................................. 8

Troubleshooting and Repairs................................................................................................... 9

Sample Splash ...................................................................................................................... 9

Leaks, Inaccurate Sampling .................................................................................................... 9

Specications ..................................................................................................................... 10

Replacement Parts: Part Numbers and Diagrams ............................................................... 11-13

Service, Calibration and Repair ............................................................................................ .14

Limited Warranty ................................................................................................................. 14

Contacting Rainin ................................................................................................................ 14

Figures

Figure 1 Rainin Classic PR-200 ............................................................................................. .3

Figure 2 Safety Filter ............................................................................................................... 4

Figure 3 Tip Immersion Depth and Tip Angle ............................................................................. 5

Figure 4 Operating Rainin Classic ............................................................................................ 5

Figure 5 Removing Tip Ejector Arm........................................................................................... 8

Contents of Rainin Classic box

Rainin Classic as ordered Quick Reference Guide

Rainin Test Report / Conformance Certicate

If any item is missing please call 800-472-4646 in the US, or contact your local MT ofce or distributor.

Technical Assistance: 800-543-4030
E-mail: [e-mail]

Made in the USA by Mettler-Toledo Rainin, LLC.

Copyright 2005-2017, Mettler-Toledo Rainin, LLC.

Rainin is a registered trademark and Rainin-Classic is a trademark of Mettler-Toledo Rainin LLC.

Rainin Classic pipettes are manufactured under U.S. Patent # 5,614,153.

Prices and specications are subject to change without notice.

Rainin Classic

™

Rainin Classic

3

Description

Eight models of the Rainin Classic continuously adju­stable digital pipette cover the entire volume range from

0.1 μL to 10 mL. Rainin Classic pipettes are not limited
to xed volume increments but can be set to any volume
in range, e.g. 6.6, 133.3, 377, 2228 μL. The piston

stroke is set by adjusting a micrometer coupled directly

to a digital volume indicator, which reads in microliters.
The digital volume indicator simplies volume setting

and virtually eliminates calculation errors.

All 2 to 5000 μL Rainin Classic models are equipped
with a stainless steel piston and an o-ring sealing sys­tem, which only needs a small amount of oil to function.

The seal in the 10 mL model requires grease for
lubrication.

A stainless steel tip ejector is provided with all models
(except PR-5000 and PR-10ML) for safe disposal of

used tips. The ejector has a quick-release mechanism.

Autoclaving

The shaft and tip ejector are autoclavable: 121°C, 1 bar,

15–20 minutes. (PR-5000, PR-10ML do not use tip

ejectors.)

Volume Indicator

The volume indicator is read from top to bottom. Up to

PR-200, black digits indicate microliters and red digits
tenths and hundredths of microliters. For PR-1000 and
PR-5000, red digits indicate milliliters and black digits
microliters. For PR-10ML, black digits indicate milliliters
and red digit tenths of a milliliter.

Sample values, volume ranges, and smallest incre-

ments for Rainin Classic models are shown below:

Figure 1: Rainin Classic

PLUNGER BUTTON
VOLUME SET

TIP EJECTOR
QUICK
RELEASE

DISPOSABLE
TIP

SHAFT

FRICTION RING
AND VOLUME
ADJUSTMENT

VOLUME
INDICATOR

RUGGED
HIGH-IMPACT
LOW THERMAL
CONDUCTIVITY
POLYMER

STAINLESS STEEL
TIP EJECTOR ARM
EXCEPT 5 and 10 mL
MODELS

TIP EJECTOR
BUTTON

4

Safety Filter, PR-5000 and PR-10ML

Models PR-5000 and PR-10ML use a safety filter in the shaft to help prevent liquid entering the shaft and

contacting the piston. If the filter gets wet, replace it. For PR-5000 insert the small diameter into the shaft;
for PR-10ML insert the large diameter into the shaft. Part numbers: 17001944 (pack of 100) and 17001945
(pack of 1000).

Tip Selection

Rainin Classic pipettes are calibrated using Rainin tips. Performance to published specications is

guaranteed only when Rainin tips are used. Graduation marks on each Rainin tip are useful for quick
volume checks.

Rainin pipettes and tips are designed together as a pipetting system. Molded from premium-grade virgin polypro­pylene, all Rainin tips are BioClean and totally inert, so you can be assured of the best pipetting results.

To mount a tip, press the shaft into the end of the tip with light force. The tip will seal properly on the shaft with

minimal force — do not use more force than is required.

n Tips must seal properly on the shaft to assure an air-tight seal and avoid leaks or poor accuracy.

n Tips must be soft and flexible so that the shaft is not scratched or worn prematurely.

n Tips must be free from microscopic flash and particulates.

n The tip orifice must be the correct size, and orifice size and geometry must be consistent from tip to tip.

Otherwise, accuracy and precision will be affected.

n Interior and exterior surfaces must be clear, smooth, and hydrophobic to avoid retention of liquid. Too much

retention results in poor accuracy and reproducibility.

Genuine Rainin tips easily pass these requirements. Rainin cannot accept responsibility for poor performance
resulting from the use of tips by other manufacturers.

Tip Immersion Depth

The recommended depth for tip insertion into the sample for each model is shown below.

Nominal Volume Volume Range Immersion Depth

2 µL 0.1 - 2 µL 1-2 mm
10 µL 0.5 - 10 µL 1-2 mm
20 µL 2 - 20 µL 2 - 3 mm
100 µL 10 - 100 µL 2 - 3 mm
200 µL 20 - 200 µL 3 - 6 mm
1000 µL 100 - 1000 µL 3 - 6 mm
5000 µL 500 - 5000 µL 6 - 10 mm
10 mL 1 mL - 10 mL 6 - 10 mm

Tip immersion depth is critical and should not be exceeded, or the volume measured may be inaccurate, possibly
out of specication.

Rainin Classic

Figure 2: Safety Filter

5

Tip Angle

The tip angle is also important – the pipette should always be used in a position within 20 degrees of vertical.
See Figure 3 below.

Operation

Before pipetting valuable samples, it is good to practice aspirating and dispensing water.

1. Turn the plunger button or the volume adjustment knob until the volume indicator is 1⁄3 revolution above the
desired setting, then turn slowly clockwise until the desired volume shows on the indicator.

2. ALWAYS dial down to the desired volume. This prevents mechanical backlash from affecting accuracy. If you
pass the desired setting, turn the dial 1⁄ 3 revolution higher than desired and dial down to reset the volume.

The friction ring prevents unintentional volume changes.

3. Attach a new disposable tip to the pipette shaft. Press into the tip with only enough force to make a positive

airtight seal.

4. Press the plunger to the FIRST STOP. This part of the stroke is the volume displayed on the indicator.

5. Holding Rainin Classic vertically, immerse

the tip into the sample to the proper depth;

see table on page 4.

6. Allow the plunger to return slowly to the UP

position. Never let it snap up! Figure 4A.

7. Pause briefly to ensure that the full volume of

sample is drawn into the tip.

8. Withdraw the tip from the sample liquid.

If any liquid remains on the outside of the

tip, wipe it carefully with a lint-free tissue,

avoiding the tip orifice.

9. To dispense sample, touch the tip end

against the side wall of the receiving vessel
and depress the plunger slowly to the first

stop. Figure 4B.

Wait 1 second: PR-2 – PR-200

Wait 1-2 secs: PR-1000,
Wait 2-3 secs: PR-5000, PR-10ML

(more for viscous solutions). Then press the

plunger to the SECOND STOP (bottom of

stroke) expelling any residual liquid in the tip.

Figure 4 Operating Rainin Classic

TOP OF

STROKE

1st STOP

2nd STOP

TIP

ANGLE

Figure 3: Tip Immersion Depth and Tip Angle