Mettler Toledo Pos-D Operating Instructions Manual
Operating Instructions
Pos-D
Positive-Displacement Pipette
™
Continuously-adjustable
6 models with
volume ranges from
0.5 to 1000 µL
Pos-D 250 µL shown
2
Positive-Displacement Pipette
Intent of Use
Pos-D positive-displacement pipettes are intended for dispensing liquids with high densities,
viscosities or vapor pressure in the volume ranges from 0.5 µL to 1000 µL in various in-vitro
liquid handling applications.
Table of Contents
Introduction ....................................................................................................... 3
Description ........................................................................................................ 4
Note on Calibration and Operation ....................................................................... 4
Capillary/Piston Assembly .................................................................................. 4
Attaching the Capillary/Piston Assembly ............................................................... 5
Volume Ranges ................................................................................................. 6
Setting the Volume ............................................................................................. 6
Pre-Rinsing ....................................................................................................... 6
Pipetting ........................................................................................................... 7
GLP (Good Laboratory Practices) ........................................................................ 7
Troubleshooting ................................................................................................. 8
Cleaning your pipette ......................................................................................... 8
Storage ............................................................................................................. 9
Service, Calibration, and Repair ........................................................................... 9
Specifications .................................................................................................. 10
Replacement Parts ............................................................................................11
Limited Warranty ................................................................................. Back Cover
Figures
Figure 1 Pos-D 250 µL ..................................................................................3
FIgure 2 Capillary/Piston ................................................................................ 4
Figure 3 Attaching Capillary/Piston .................................................................. 5
Figure 4 Volume Display ................................................................................ 6
Figure 5 1st and 2nd Stops ............................................................................ 7
Figure 6 Replacement parts ...........................................................................11
Contents of Pos-D box
Pos-D pipette as ordered
ID tags Rainin Test Report / Conformance Certicate
If any item is missing please call 800-472-4646 in the US, or contact your local MT ofce
or distributor.
Made in the USA by Mettler-Toledo Rainin, LLC.
Rainin is a registered trademark and Pos-D and Hang-Ups are trademarks of Mettler-Toledo Rainin LLC.
Pos-D
™
Pos-D
3
Introduction
Pos-D is a positive displacement pipette for demanding biological protocols and for use with
liquids with non-aqueous properties: e.g. viscous, dense, volatile, or high-surface-tension
liquids. Positive-displacement pipettes have certain advantages over air-displacement
pipettes for these applications:
n Concerning DNA amplification protocols, the positive-displacement Pos-D absolutely
prevents sample-to-sample contamination from aerosols. Disposable capillaries and
pistons prevent contamination from carry-over from one sample or reagent to another.
Each capillary and piston is used only once and ejected without hand contact.
n Concerning non-aqueous samples, because the piston is in direct contact with the sample
(no partial vacuum),viscous, dense, volatile, or high-surface-tension liquids are measured
accurately and reproducibly.
PLUNGER BUTTON (COLORCODED LABEL SHOWS MODEL)
PLUNGER
VOLUME
SETTING
KNOB
DIGITAL VOLUME DISPLAY
BODY
SHAFT
CAPILLARY/PISTON ASSEMBLY
PISTON CLAMP (IN SHAFT)
FINGERHOOK
Figure 1: Pos-D, 250 µL
4
Description – Additional Features
Permanent calibration.
The digital volume setting allows precise and continuous adjustment, and faces the user
during the pipetting operation.
No lubrication or maintenance is required.
The fingerhook allows you to rest your hand and reduces pipetting static forces.
Note on Calibration and Operation
Pos-D is calibrated at Rainin during manufacturing.
When the capillary/piston assembly is fitted correctly, the pipette will perform to the accuracy
and precision specification as indicated in this manual.
Capillary/Piston Assembly
Pos-D capillaries/pistons are supplied already assembled, pre-sterilized, in racks. The
following illustration shows the parts assembled and as they would appear if unassembled.
(250 µL shown, other volumes are similar.)
Pos-D
PISTON
CAPILLARY
ASSEMBLED
CAPILLARY/
PISTON
SEAL
END
PISTON
STEM
Figure 2: Attaching Capillary/Piston
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