Memmert ICO 240, ICO 50, ICO 105, ICO 150 Instruction Manual

ICO

OPERATING MANUAL

CO2 INCUBATOR ICO

Manufacturer and customer service

MEMMERT GmbH + Co. KG
Willi-Memmert-Straße 90-96
91186 Büchenbach
Germany

Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.memmert.com

Customer service:
Service hotline: [phone]
Service fax: [phone]
E-mail: [e-mail]

For service enquiries, please always specify the appliance number on the nameplate (see page

12).
Shipping address for repairs:

Memmert GmbH + Co. KG
Customer service
Willi-Memmert-Str. 90-96
DE-91186 Büchenbach
Germany

Please contact our customer service before sending appliances for repair or before returning
equipment, otherwise, we have to refuse acceptance of the shipment.

© 2016 MEMMERT GmbH + Co. KG
D33451 | Edition 11/2016
Changes reserved

About this manual

About this manual

Purpose and target audience

This manual describes the design, function, transport, putting into operation, the actual op­eration and maintenance of CO
the owner, who have the task of operating and/or maintaining the respective appliance.

If you are asked to work on the appliance, read this manual carefully before starting. Familiar­ise yourself with the safety regulations. Only perform work that is described in this manual. If
there is something you do not understand, or certain information is missing, ask your man­ager or contact the manufacturer. Do not do anything without authorisation.

Versions

The appliances are available in different configurations and sizes. If specific equipment fea­tures or functions are available only for certain configurations, this is indicated at the relevant
points in this manual.

The functions described in this manual refer to the latest firmware version.
Due to individual configurations and sizes, illustrations in this manual may be slightly different

from the actual appearance. Function and operation are identical.

Other documents that have to be observed

When operating the appliance with the MEMMERT AtmoCONTROL PC software, observe the
separate software manual. To open the AtmoCONTROL software manual, click on “Help” in
the AtmoCONTROL menu bar.

Storage and resale

This instruction manual belongs with the appliance and should always be stored where
persons working on the appliance have access to it. It is the responsibility of the owner to
ensure that persons who are working or will work on the appliance are informed as to the
whereabouts of this instruction manual. We recommend that it is always stored in a protected
location close to the appliance. Make sure that the instruction manual is not damaged by heat
or humidity. If the appliance is resold or transported and then set up again at a different loca­tion, the operating instructions must go with it.

For the current version of this operating manual in pdf format, please go to http://www.mem­mert.com/en/service/downloads/user-manual/ .

incubators ICO. It is intended for use by trained personnel of

2

D33451 | Edition 02/2017 3

Contents

Contents

1. For your safety 6

1.1 Terms and signs used........................................................................................................... 6

1.2 Product safety and dangers ................................................................................................ 7

1.3 Requirements of the operating personnel .......................................................................... 8

1.4 Responsibility of the owner ................................................................................................. 8

1.5 Intended use ........................................................................................................................ 8

1.6 Changes and conversions ....................................................................................................9

1.7 Behaviour in case of malfunctions and irregularities ..........................................................9

1.8 What to do in case of accidents .......................................................................................... 9

1.9 Switching off the appliance in an

emergency ........................................................................................................................... 9

2. Construction and description 10

2.1 Construction ...................................................................................................................... 10

2.2 Description and function ................................................................................................... 10

2.3 Material.............................................................................................................................. 11

2.4 Electrical equipment .......................................................................................................... 11

2.5 Connections and interfaces ...............................................................................................11

2.6 Designation (nameplate) ................................................................................................... 12

2.7 Technical data .................................................................................................................... 13

2.8 Applied directives and standards ...................................................................................... 14

2.9 Declaration of conformity ................................................................................................. 14

2.10 Ambient conditions ........................................................................................................... 15

2.11 Scope of delivery ...............................................................................................................15

2.12 Optional accessories .......................................................................................................... 15

3. Delivery, transport and setting up 16

3.1 For your safety ...................................................................................................................16

3.2 Delivery .............................................................................................................................. 17

3.3 Transport ............................................................................................................................ 17

3.4 Unpacking ......................................................................................................................... 17

3.5 Storage after delivery ........................................................................................................17

3.6 Setting up .......................................................................................................................... 18

4. Putting into operation 20

4.1 Connect the appliance to the power supply ..................................................................... 20

4.2 Establishing water supply .................................................................................................. 20

4.3 CO

4.4 Switching on ...................................................................................................................... 22

5. Operation and control 23

5.1 For your safety ...................................................................................................................23

5.2 Operating personnel.......................................................................................................... 23

5.3 Opening the door .............................................................................................................. 24

5.4 Loading the appliance .......................................................................................................25

5.5 Operating the appliance .................................................................................................... 25

5.6 Monitoring function .......................................................................................................... 32

5.7 Graph ................................................................................................................................. 38

5.8 Sterilising the appliance ...................................................................................................40

5.9 Ending operation ...............................................................................................................40

and N2 connection ...................................................................................................... 21

2

4 D33451 | Edition 11/2016

Contents

6. Malfunctions, warning and error messages 41

6.1 Warning messages of the monitoring function ................................................................ 41

6.2 Malfunctions, operating problems and appliance errors ................................................ 43

6.3 Power failure ...................................................................................................................... 45

7. Menu mode 46

7.1 Overview ............................................................................................................................ 46

7.2 Basic operation in menu mode using the example of language selection ....................... 47

7.3 Setup.................................................................................................................................. 48

7.4 Date and time .................................................................................................................... 51

7.5 Calibration ......................................................................................................................... 52

7.6 Programme ........................................................................................................................ 58

7.7 Sounds ............................................................................................................................... 59

7.8 Protocol ............................................................................................................................. 60

7.9 USER ID .............................................................................................................................. 61

8. Maintenance and Servicing 63

8.1 Regular maintenance.........................................................................................................63

8.2 Cleaning ............................................................................................................................. 63

8.3 Repairs and Service ............................................................................................................ 63

9. Storage and disposal 64

9.1 Storage .............................................................................................................................. 64

9.2 Disposal ............................................................................................................................. 64

Index 65

D33451 | Edition 11/2016 5

For your safety

1. For your safety

1.1 Terms and signs used

In this manual and on the appliance itself, certain common terms and signs are used to warn
you of possible dangers or to give you hints that are important in avoiding injury or damage.
Observe and follow these notes and regulations to avoid accidents and damage. These terms
and signs are explained below.

1.1.1 Terms used

WARNING

CAUTION

NOTICE

1.1.2 Signs used

Danger of elec-

trocution

Do not tilt Disconnect the

Information on

first aid

Fire hazard

mains plug

First aid:

Rinse eyes out

Warns about a dangerous situation that might lead to death
or serious injuries

Warns about a dangerous situation that might lead to mod­erate or minor injuries

Warns about material damage

Gases / vapours Danger of frost-

bite/

cold burns

Wear gloves Wear safety

boots

Important or

useful

additional infor-

mation

Warning about

gas bottles

Observe informa-

tion in separate

manual

6 D33451 | Edition 11/2016

For your safety

1.2 Product safety and dangers

The appliances described in this manual are technically sophisticated, manufactured using
high-quality materials and subject to many hours of testing in the factory. They reflect the
state of the art and comply with recognised technical safety regulations. However, there are
still risks involved, even when the appliances are used as intended. These are described below.

WARNING

After removing covers, live parts may be exposed. You may receive
an electric shock if you touch these parts. Disconnect the mains plug
before removing any covers. Only electrical engineers may work on
the electrical equipment of the appliances.

WARNING

When loading the appliance with an unsuitable load, poisonous or
explosive vapours or gases may be produced. This could cause the
appliance to explode, and people could be severely injured or poi­soned. The appliance may not be loaded with materials / test objects
that release toxic or explosive vapours when heated up (see also
chapter Intended use on page 8).

WARNING

Leaving the door open during operation can cause the appliance to
overheat or pose a fi re hazard. Do not leave the door open during
operation.

WARNING

Gas bottles may burst or explode at high temperatures. Keep the
gas bottles away from open fl ames. Store gas bottles below 50 °C
and ensure that the location is always well ventilated. Prevent water
from penetrating as well as backfl ow into the gas bottles. It is es-
sential that you read the safety notes and instructions of the gas
supplier.

CAUTION

Danger of suffocation. CO2 and N2 can have a suffocating effect
in high concentrations. In normal mode, the appliance emits small
amounts of CO2 and N2 to its surroundings. You should therefore
ensure that the room in which it is installed is properly ventilated.
Always close the stop valve or pressure reducer on the gas bottles if
the appliance is not in operation.

CAUTION

High concentrations of CO2 can cause cold burns or frostbite. Avoid
contact with CO2 gas to the eyes and skin.

D33451 | Edition 11/2016 7

For your safety

CO2 and N2 are not dangerous substances in terms of the German Hazardous Substances
Ordinance (GefStoffV). You should nevertheless familiarise yourself with the applicable safety
regulations prior to handling such gas bottles.

1.3 Requirements of the operating personnel

The appliance may only be operated and maintained by persons who are of legal age and
have been instructed accordingly. Personnel who are to be trained, instructed or who are un­dergoing general training may only work with the appliance under the continuous supervision
of an experienced person.

Repairs may only be performed by qualified electricians. The regulations in the separate service
manual must be observed.

1.4 Responsibility of the owner

The owner of the appliance

► is responsible for the flawless condition of the appliance and for it being operated in ac-

cordance with its intended use (see chapter 1.5);

► is responsible for ensuring that persons who are to operate or service the appliance are

qualified to do this, have been instructed accordingly and are familiar with the operating
instructions at hand;

► must know about the applicable guidelines, requirements and operational safety regula-

tions, and train staff accordingly;

► is responsible for ensuring that unauthorised persons have no access to the appliance;
► is responsible for ensuring that the maintenance plan is adhered to and that maintenance

work is carried out properly (see page 63);

► has to ensure that the appliance and its surroundings are kept clean and tidy, for example

through corresponding instructions and inspections;

► is responsible for ensuring that personal protective clothing is worn by operating person-

nel, e.g. work clothes and safety shoes.

1.5 Intended use

CO2 incubators ICO are intended for incubation of cell cultures or similar.
The appliance is not explosion-proof (does not comply with the German occupational health

& safety regulation VBG 24). The appliance may only be loaded with materials and substances
which cannot form any toxic or explosive vapours at the set temperature and which cannot
explode, burst or ignite.

The appliance may not be used for the drying, evaporation and baking of paints or similar
materials, the solvents of which could form an explosive mixture when combined with air. If
there is any doubt as to the composition of materials, they must not be loaded into the appli­ance. Potentially explosive gas-air mixtures must not form, neither in the working chamber nor
in the direct vicinity of the appliance.

Only feed distilled water as well as CO
tions on the rear of the appliance. Introducing other liquids or gases is not permitted.

The incubator may not be used for sterilisation purposes. It is not a steriliser according to the
German Law on Medical Products. Sterilisation programmes (see page 58) that are saved
in the appliance only serve to sterilise the appliance itself. Do not use them to sterilise medical
devices!

8 D33451 | Edition 11/2016

and N2 into the chamber through the media connec-

2

For your safety

OONN

N

1.6 Changes and conversions

No unauthorised changes or alterations may be made to the appliance. No parts may be
added or inserted which have not been approved by the manufacturer.

Unauthorised changes or alterations result in the CE declaration of conformity losing its valid­ity, and the appliance may no longer be operated.

The manufacturer is not liable for any damage, danger or injuries that result from unauthor­ised changes or alterations, or from non-observance of the regulations in this manual.

1.7 Behaviour in case of malfunctions and irregularities

The appliance may only be used in a flawless condition. If you as the operator notice irregu­larities, malfunctions or damage, immediately take the appliance out of service and inform
your superior.

You can find information on eliminating malfunctions from page 41.

1.8 What to do in case of accidents

1. Keep calm. Act with determination and consideration. Pay attention to your
own safety.

2. Switch off the appliance and close the valves of the gas bottle.

3. Call a doctor.

4. Start first aid measures. If available: Call a trained first aid helper.

In case of contact with CO2 to the eyes and skin:

Rinse eyes out with water for at least 15 minutes. In case of cold burns, rinse with
water for at least 15 minutes. Cover over in a sterile way. Call a doctor.

When breathing in CO2 or N2:

High concentrations can cause suffocation. Symptoms may include a loss of mobility and
unconsciousness. The victim is not aware of suffocating.

Low concentrations of CO

can cause accelerated breathing and headaches.

2

Anyone affected should breathe fresh air, using a breathing device independent of recircu­lating air. Keep the person warm and calm. Call a doctor. In case of respiratory arrest, use
artificial respiration.

In case of gas leakage:

Leave the room immediately, warn others and ventilate the room. If you re-enter the room,
use a breathing device independent of recirculating air if it has not been established that the
atmosphere is harmless.

1.9 Switching off the appliance in an
emergency

Press the main switch at the ControlCOCKPIT (Fig. 1) and
disconnect the power plug. This disconnects the appliance
from the power supply at all poles.

Fig. 1
Switch off the appliance by press­ing the main switch

D33451 | Edition 11/2016 9

ON

Construction and description

2. Construction and description

2.1 Construction

1
2

3
4

5

6

Fig. 2 Construction of CO2 incubators ICO

1 ControlCOCKPIT with capacitive function

keys and LCD displays (see page 26)
2 On/Off switch (see page 22)
3 Inner glass door

4 Stainless steel perforated sheet
5 Water tray (passive humidity control)
6 Adjustable feet
7 Nameplate (see page 12)

7

2.2 Description and function

Air is heated inside the appliance by means of large-area all-round heating.
The interior of appliances with passive humidity control is humidified with water that evapo-

rates from a tray that is placed inside. The interior of appliances with active humidity control
is humidified with water evaporating at a set rate from a tank by means of a hot-air generator
on the rear side of the appliance. The sterile hot air is introduced into the interior above the
fan and mixed with the air current. In appliances with water trays, a Peltier humidity trap in
the back of the appliance limits humidity. Appliances with active humidity are dehumidified
with a dosed supply of fresh air provided through a sterile filter.

Carbon dioxide (CO
the interior through sterile filters. Interior ventilation ensures a uniform distribution of the
gases, creating a homogeneous atmosphere. The oxygen concentration is controlled by intro­ducing nitrogen: If nitrogen is introduced, the concentration of oxygen decreases.

) and nitrogen (N2 only for models with O2 module) are also injected into

2

10 D33451 | Edition 11/2016

Construction and description

2.3 Material

For the outer housing, MEMMERT uses stainless steel (Mat.No. 1.4016 – ASTM 430) and for
the interior, stainless steel (Mat.No. 1.4301 – ASTM 304) is used, which stands out through
its high stability, optimal hygienic properties and corrosion resistance to many (but not all!)
chemical compounds (caution for example with chlorine compounds).

The chamber load for the appliance must be carefully checked for chemical compatibility with
the materials mentioned. A material resistance table can be requested from the manufacturer.

2.4 Electrical equipment

► Operating voltage and current consumption: See nameplate
► Protection class I, i.e. operating insulation with PE conductor in accordance with EN 61010
► Protection type IP 20 acc. to EN 60 529
► Interference suppression acc. to EN 55011 class B
► Appliance fuse: Safety fuse 250 V/15 A, quick-blow
► The temperature controller is protected with a miniature fuse 100 mA (160 mA at 115 V)

2.5 Connections and interfaces

2.5.1 Electrical connection

This appliance is intended for operation on an electrical power system with a system imped­ance Z
must ensure that the appliance is operated only on an electrical power system that meets
these requirements. If necessary, you can ask your local energy supply company what the
system impedance is.

Observe the country-specific regulations when making connections (e.g. in Germany DIN VDE
0100 with earth leakage circuit breaker).

of a maximum of 0.292 ohm at the point of transfer (service line). The operator

max

2.5.2 Communication interfaces

The communication interfaces are intended for appliances which meet the requirements of
IEC 60950-1.

USB interface

The appliance is fitted by default with a USB
interface in accordance with the USB specifica­tion. This way, you can

► transfer software stored on a USB storage

medium to the appliance (see page 58).

► export protocol logs from the appliance to

a USB storage medium (see page 60).

► transfer user ID data stored on a USB stor-

age medium to the appliance (see page

61).

The USB port is located on the right side of the ControlCOCKPIT (Fig. 3).

D33451 | Edition 11/2016 11

Fig. 3 USB interface

Construction and description

Ethernet interface

Via Ethernet interface, the appliance can
be connected to a network, so that you
can transfer programmes created with the
AtmoCONTROL software to the appliance and
read out protocols. The Ethernet interface is
located on the rear of the appliance (Fig. 4).

For identification purposes, each appliance con­nected must have its own unique IP address.
Setting the IP address is described on page
48 .

You will find a description of how to transfer programmes via Ethernet in the en­closed AtmoCONTROL manual.

With an optional USB to Ethernet converter, the appliance can be directly connected to a com­puter / laptop (see Optional accessories on page 15).

Fig. 4 Ethernet interface

2.6 Designation (nameplate)

The nameplate (Fig. 5) provides information about the appliance model, manufacturer and

technical data. It is attached on the upper right behind the door (see page 10).

1
2

3
4

5

6

Fig. 5 Nameplate (example)
1 Type designation

2 Operating voltage
3 Applicable standard
4 Protection type
5 CE conformity

12 D33451 | Edition 11/2016

Typ: ICO 240 F.-Nr.: O616.0088
230 V

~

8.7

50/60 Hz 2000 W

DIN12880-Kl.3.3 Nenntemp.: +18 - +50 °C

6 Address of manufacturer
7 Disposal note
8 Temperature range
9 Connection / power ratings
10 Appliance number

10

9
8

7

Construction and description

2.7 Technical data

Appliance size 50 105 150 240

Appliance width D* [mm] 559 719 719 759
Appliance height E* [mm]

(varies due to adjustable feet)
Appliance depth F* (without door handle) [mm] 521 591 591 691
Depth of door handle [mm] 56
Chamber width A* [mm] 400 560 560 600
Chamber height B* [mm] 425 480 700 810
Interior depth C* [mm] (less 35 mm for fan) 330 400 400 500
Chamber volume [litres] 56 107 156 241
Net weight [kg] 55 75 90 110
Weight including packaging [kg] 74 100 116 145
Power [W] 1000 1500 2000 2000
max. number of sliding shelves 5 6 10 12
max. load per sliding shelf [kg] 15

max. load per appliance [kg] 75 90 120 140

Operating temperature range

Setting temperature range [°C] +18 to +50

Adjustment precision [°C] 0.1
Temporal temperature deviation

(in accordance with DIN 12880:2007-05) at 37 °C
[K]

Spatial temperature deviation at +37 °C
(in accordance with DIN 12880:2007-05) at 37 °C
[K]

Adjustment range of active humidity control
[% rh]
(only for appliances with the corresponding con­figuration)

Setting accuracy humidity [%] 0.5

Adjustment range for CO

Setting accuracy for CO
Adjustment range for O

appliances with active humidity control)
Setting accuracy for O

[%]

2

[%]

2

[%] (optional and only for

2

[%]

2

* see Fig. 6 on page 14 .

791 846 1066 1176

5 °C above room temperature up to

50 °C

±0.1

±0.3

40 to 97 and rh off

0 to 20

0.1

1 to 20

0.1

D33451 | Edition 11/2016 13

Construction and description

D

F

C

E

B

A

Fig. 6 Dimensions

90

G

2.8 Applied directives and standards

► Directive 2004/108/EC amended (Directive of the council for harmonisation of the laws of

the member states on electromagnetic compatibility). Standards complied with:

DIN EN 61326:2004-05, EN 61326:1997, EN 61326/A1:1998, EN 61326/A2:2001

EN 61326/A2:2003

► Directive 2006/95/EC amended (Directive of the council for harmonisation of the laws of

member states relating to electrical equipment designed for use within certain voltage

limits). Standards complied with:

DIN EN 61 010-1 (VDE 0411 part 1)

DIN EN 61 010-2-010 (VDE 0411 part 2-010)

EN 61 010-1:2001, EN 61 010-2-010

56

2.9 Declaration of conformity

You can download the EC declaration of conformity of the appliance online:
English: http://www.memmert.com/en/service/downloads/ce-statement/
German: http://www.memmert.com/de/service/downloads/eg-konformitaetserklaerung/

14 D33451 | Edition 11/2016

Construction and description

2.10 Ambient conditions

► The appliance may only be used in enclosed areas and under the following ambient condi-

tions:

Ambient temperature

Humidity rh max. 70 %, non-condensing

Overvoltage category II

Pollution degree 2

Altitude of installation max. 2,000 m above sea level

10 °C to 35 °C

► The appliance may not be used in areas where there is a risk of explosion. The ambient air

must not contain any explosive dusts, gases, vapours or gas-air mixtures. The appliance is

not explosion-proof.

► Heavy dust production or aggressive vapours in the vicinity of the appliance could lead to

sedimentation in the interior and, as a consequence, could result in short circuits or dam-

age to electrical parts. For this reason, sufficient measures to prevent large clouds of dust

or aggressive vapours from developing should be taken.

2.11 Scope of delivery

► Power cable
► 1 or 2 stainless steel perforated sheets (load capacity: 15 kg each)
► 1 stainless steel water tray (only for appliances with passive humidity control)
► 1 water tank (only for appliances with active humidity control)
► Gas connection hose (depending on the model: one to three)
► USB storage medium with software and AtmoCONTROL manual
► the operating instructions at hand
► Calibration certificates
► Separately packaged fastening material for wall mounting (see page 19)

2.12 Optional accessories

► USB to Ethernet converter (Fig. 7). Makes it

possible to connect the Ethernet connection

interface (see page 12) to the USB port of

a computer / laptop.

Fig. 7 USB to Ethernet converter

D33451 | Edition 11/2016 15

Delivery, transport and setting up

3. Delivery, transport and setting up

3.1 For your safety

WARNING

Because of the heavy weight of the appliance, you could injure your­self if you try to lift it. To carry appliances of size 50, at least two
persons are needed; for appliances of the sizes 105 and 150, four
people are needed. Appliances larger than that may not be carried
but must be transported with a manual pallet jack or forklift truck.

CAUTION

50

You might get your hands or feet squashed when transport­ing and installing the appliance. Wear protective gloves and
safety boots. When grasping the bottom of the appliance,
grasp it only on the sides:

105

150 240

9

16 D33451 | Edition 11/2016

Delivery, transport and setting up

3.2 Delivery

The appliance is packed in cardboard and is delivered on a wooden palette.

3.3 Transport

The appliance can be transported in the following ways:

► With a forklift truck; move the forks of the truck entirely under the pallet.
► On a manual pallet jack

3.4 Unpacking

NOTICE

► To avoid damage, do not unpack the appliance until you reach the installation site.

Remove the cardboard packaging by pulling it upwards or carefully cutting along an edge.

3.4.1 Checking for completeness and transport damage

► Check the delivery note to ensure that the delivery is complete.
► Check the appliance for damage.

If you notice deviations from the delivery note, damage or irregularities, do not put the appli­ance into operation but inform the haulage company and the manufacturer.

3.4.2 Removing the transport protection

Remove the transport protection. It is located between the door hinge, door and frame and
has to be removed after opening the door.

3.4.3 Disposing of packaging material

Dispose of the packaging material (cardboard, wood, foil) in accordance with the applicable
disposal regulations for the respective material in your country.

3.5 Storage after delivery

If the appliance is first to be stored after delivery: Read the storage conditions from page

64.

D33451 | Edition 11/2016 17

Delivery, transport and setting up

3.6 Setting up

3.6.1 Preconditions

The installation site must be flat and horizontal and must be able to reliably bear the weight
of the appliance (see Technical data on page 13). Do not place the appliance on a flamma­ble surface.

The appliance emits small amounts of CO
fore, the installation site must be ventilated.

Depending on the model (see nameplate), a 230 V or 115 V power connection must be avail­able at the installation site.

The distance between the wall and the rear of the appliance must be at least 15 cm. The clear­ance from the ceiling must not be less than 20 cm and the side clearance from walls or nearby
appliances must not be less than 5 cm (Fig. 8). Sufficient air circulation in the vicinity of the
appliance must be guaranteed at all times.

and N2 to its surroundings during operation. There-

2

FP

FP FP

Fig. 8 Minimum clearance from walls and ceiling

18 D33451 | Edition 11/2016

FP

Delivery, transport and setting up

3.6.2 Tilt protection

Due to its centre of gravity, the appliance can fall over to the front and injure you or other
people. Always attach the appliance to a wall with the tilt protection included in the delivery.
In case there is not enough space, do not put the appliance into operation and do not open
the door. Contact the Memmert service (see page 2).

1. Screw the tilt protection

onto the back of the ap-

pliance as illustrated.

2. Bend the tilt protec-

tion upwards by 90 ° in

the desired distance to

the wall (consider the

minimum distance to the

wall, see Fig. 8).

3. Drill a hole, insert a plug

and screw the tilt protec-

tion to a suitable wall.

D33451 | Edition 11/2016 19

Putting into operation

4. Putting into operation

NOTICE

The first time the appliance is operated, it must not be left unattended until it has reached the
steady state.

4.1 Connect the appliance to the power supply

Observe the country-specific regulations when making
connections (e.g. in Germany: DIN VDE 0100 with earth
leakage circuit breaker). Observe the connection and
power ratings (see nameplate and "Technical Data" on
page 13). Make sure to establish a safe PE conductor
connection.

Plug the provided power cable into the rear of the appli­ance and connect it to the power supply ( Fig. 9 ). Place the
power cable so that

► it is easily accessible at all times and can be pulled off quickly, for example in case of inter-

ference or an emergency;

► it does not represent a trip hazard;
► it cannot come into contact with any hot parts.

4.2 Establishing water supply

4.2.1 Water specifi cations

For humidification with a water tray or steam generator, only use: demineralised water (aqua
dem) in accordance with VDE 0510/DIN EN 50272 if you are working according to these
regulations (production conductivity ≥ 5 ≤ 10 μS/cm). You can buy battery water in accord­ance with the VDE 0510 standard in all larger drug stores, supermarkets and hardware stores
as well as from wholesale traders. The VDE 0510/DIN EN 50272 standard has to be explicitly
specified on the label.

Otherwise, possible calcification in the steam generators, steam piping and steam generator
pumps could affect the correct functioning of the appliance.

Only use chlorine-free water with a pH value of > 5 and < 7.

Fig. 9 Connect the power
cable to the rear of the appliance

4.2.2 For appliances with passive humidity control: inserting the water tray

Mount the provided sealing lip to the narrow side of the water tray (Fig. 10) and fill 1.5 cm to
2 cm of water into the tray (for specifications see section 4.2.1).

Place the water tray with the attached sealing lip in the centre of the appliance floor and
carefully push it towards the rear panel until the sealing lip is completely under the ventilation
opening, touching the rear panel along its entire length (Fig. 11). The sealing lip magnetically
adheres to the rear panel and returns the water condensing on the humidity limiter to the
water tray.

NOTICE

Important: make sure not to spill any water and make sure that no water flows past the water
tray and onto the floor, as this would cause the humidity to exceed the maximum values.

20 D33451 | Edition 11/2016

Putting into operation

Fig. 10 Attach the sealing lip

Fig. 11 Push the water tray to the rear
panel

4.2.3 For appliances with active humidity control: Fill up and connect the water

tank

Fill the supplied water tank with water and use the enclosed tube to connect it to the “H2O”
connection on the rear of the chamber (Fig. 12).

4.3 CO2 and N2 connection

WARNING

Danger of explosion and poisoning when introducing gases/sub­stances other than CO2 and N2. Only carbon dioxide (CO2) and ni­trogen (N2) may be introduced into the appliance through the gas
connections on the rear of the appliance.

WARNING

Gas bottles may burst or explode at high temperatures. Keep the gas
bottles away from open fl ames. Do not store gas bottles at or above
50 °C and ensure that the location is always well-ventilated. Prevent
water from penetrating as well as backfl ow into the gas bottles. It is
essential that you read the safety notes and instructions of the gas
supplier.

CAUTION

Danger of suffocation. CO2 and N2 can have a suffocating effect
in high concentrations. In normal mode, the appliance emits small
amounts of CO2 and N2 to its surroundings. You should therefore
ensure that the room in which it is installed is properly ventilated.
Always close the stop valve or pressure reducer on the gas bottles if
the appliance is not in operation.

CAUTION

High concentrations of CO2 can cause cold burns or frostbite. Avoid
contact with CO

D33451 | Edition 11/2016 21

gas to the eyes and skin.

2