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Impact
Operator's Manual
62 pgs613.2 Kb0

Matrix Impact2, Impact Operator's Manual

CONTENTS
Congratulations! .......................................................... 1
A. INTRODUCTION ..........................................................2
B. PIPETTOR OVERVIEW................................................4
B.1 General Description..............................................4
B.2 Keypad................................................................ 6
B.3 Display..............................................................12
B.4 Specifications ....................................................13
B.5 Hazards and Precautions ....................................14
C. PROGRAMMING AND OPERATION ........................... 15
C.1 Programming the IMPACT................................... 15
C.2 Programming the IMPACT2.................................19
C.3 Reviewing the Program........................................21
C.4 Programming Examples......................................22
C.5 Operating Procedures .........................................24
D. CALIBRATION ..........................................................26
D.1 Calibrating the IMPACT....................................... 27
D.2 Calibrating the IMPACT2.....................................30
D.3 CALVIEW ..........................................................31
D.4 CALRESET........................................................31
D.5 Recommendations for Accurate Pipetting .............32
iii
CONTENTS (cont’d)
E. TROUBLESHOOTING AND MAINTENANCE................ 33
E.1 Troubleshooting ..................................................33
E.2 Maintenance ......................................................35
E.3 Recharging Batteries ..........................................42
E.4 Replacing Batteries ............................................44
F. APPENDICES............................................................48
F.1 Additional Pipetting Examples .............................48
F.2 Chemical Compatibility........................................50
F.3 Customer Service...............................................51
G. IMPACT
2
STORED PR OGRAMS WORKSHEET ................53
iv
Congratulations!
You have purchased the state-of-the-art in precision pipetting. The
IMPACT
®
is the first cordless electronic pipettor that "understands"
the myriad of liquid handling protocols typically performed in today's laboratory.
Ergonomically designed and balanced to reduce fatigue, the
IMPACT functions so intuitively, that you may need no more than a
quick survey of this manual to use it. The IMPACT allows you to accomplish your liquid pipetting tasks
quickly, accurately, and with less risk of developing repetitive motion related injuries that are typical with manual pipettor use. Laboratory results will become more standardized, while less time is expended on tedious bench procedures.
Delivering power, precision, and performance.
is the latest word in liquid handling technology.
Before You Begin
Before using the IMPACT or IMPACT2, verify the contents of your package. The package should contain the following items:
1 IMPACT or IMPACT2 Pipettor 1 Operator's Manual 2 Rechargeable Batteries 1 AC Power Transformer 1 Warranty Card 1 Accuracy/Precision Data Sheet 1 Coupon for Free Calibration Service
If you are missing any items, contact your Matrix Technologies representative.
Note: Batteries must be installed and recharged before using the
IMPACT. Refer to the following sections in this manual:
n Section E.3, Installing/Replacing Batteries
n Section E.4, Recharging Batteries
1
A. INTRODUCTION
The IMPACT is lightweight and cordless. It provides the convenience of a manual pipettor, with the programming capabilities of an electronic pipettor. It runs on rechargeable batteries and can be used while recharging. The pipettor also has an automatic shutoff feature to extend battery life.
The IMPACT uses a "paced dispense" feature that performs repeat pipetting steps while the trigger is depressed.
The IMPACT uses integrated circuit technology to store its programming information. It will hold up to 40 steps in a program. The program information is retained in memory, even if the batteries are fully discharged or removed.
The IMPACT2 is the second generation of the IMPACT pipettor family. It is capable of performing all of the same pipetting protocols available with the IMPACT, plus a few NEW features. The expanded capabilities include:
§ Expanded memory for storing up to five pipetting programs.
Each program can hold a maximum of 40 steps.
§ “Scratch Pad” programming mode which allows for fast and
simple program changes that are not stored into memory.
§ Enhanced speed control which offers slower aspirating and
dispensing of viscous liquids. Excellent for Gel loading!
§ “EASY” CAL which simplifies in-lab calibration verification by
using distilled water.
2
INTRODUCTION
The IMPACT and IMPACT2 are available in 8-channel and 12-channel formats (see Figure A). An expandable version of the 8-channel format is also available for pipetting samples from test tubes in a rack. It comes in several models to handle fluid volumes from 0.5 µl to 1250 µl. The handles are color-coded for easy identification of different model types (IMPACT vs. IMPACT2) and different volume ranges. See Section B.4, Specifications.
8-Channel 12-Channel 8-Channel
Expandable
Figure A: IMPACT Configurations
3
B. PIPETTOR OVERVIEW
B.1 General Description
Battery Case
Pipetting Trigger
Ejector Trigger
Tip Fittings
Figure B.1: Description of Components
Display
Keypad
Transformer Connector
Color-coded Ring
Keypad. The keypad contains the operation keys that are used in programming the pipetting sequence and volumes. See Section B.2, Keypad.
Display. The display is an LCD screen that shows the pipetting operation (fill, dispense, mix, or purge) and fluid volume for each step in the pipetting program. See Section B.3, Display.
4
PIPETTOR OVERVIEW
Battery Case. The battery case contains two rechargeable nickel cadmium batteries. Batteries are charged for 12– 14 hours. When fully charged, the pipettor can perform up to 1200 cycles at full stroke. Battery life is extended with automatic shutoff after 10 minutes of inactivity. See Section E.3, Installing/ Replacing Batteries.
Pipetting Trigger. The pipetting trigger initiates the pipetting program. It is pressed when the "Run" or "Purge" command appears in the display. A single pipetting step is performed by a quick press and release of the trigger. Repeat pipetting (paced dispense) is performed by pressing and holding the trigger.
Note: At the end of a paced dispense, the pipet tips should be removed from the fluid before releasing the trigger, to prevent aspiration of fluid.
Ejector Trigger. The ejector trigger is pressed to eject the pipet tips from the pipettor.
Tip Fittings. The tip fittings form an airtight seal with the pipet tips to ensure accuracy while pipetting.
Note: To extend instrument life, Matrix Technologies has equipped all IMPACT and IMPACT2 pipettors with chemically­resistant (PEEK) tip fittings. This material allows for pipetting of acidic solutions without damage to the tip fittings.
Color-coded Ring. The flanged ring around the pipettor handle is color-coded to identify the pipetting volume of the handle. See Section B.4, Specifications.
Transformer Connector. The transformer connector receives the plug from the power transformer for recharging the batteries. The pipettor can be used while it is recharging.
5
PIPETTOR OVERVIEW
B.2 Keypad
The keypad contains the operation keys, program keys, and the special function key.
Display
Keypad
Display
Keypad
Program Keys
Special Function
Fill On
Vol
Disp
Mix
Enter
Off/
Prog
Purge
Key
Figure B.2a: IMPACT Keypad
Program Keys
Special Function Key
Fill On
Vol
Disp
Mix
Enter
Off/
Prog
Purge
Operation Keys
Operation Keys
6
Figure B.2b: IMPACT2 Keypad
Operation Keys:
PIPETTOR OVERVIEW
[On/Off]
[Speed]
The On/Off key turns the pipettor on or off. When the pipettor is turned on, it emits a beep tone, flashes "ZERO" on the display, then automatically resets the pistons to zero position. After zeroing, the display shows the first step of the program in the RUN mode.
Note: If the pipettor is being programmed for the first time, the first step will be the fill operation, with the default fill volume. See Section C.1, Program-ming the IMPACT, for the default settings.
The Speed key controls the rate at which the liquid is aspirated or dispensed. The speed setting can be changed by repeatedly pressing the Speed key.*
For the IMPACT, three speed settings are available: Speed Setting Display SLOW S F
MEDIUM S F FAST S F
For the IMPACT2, five speed settings are available: Speed Set ting Display SLOW S
MEDIUM SLOW S MEDIUM S MEDIUM FAST S FAST S F
The IMPACT2 also features independent speed adjustment. Speed settings can be changed within a stored program or during the execution of a program (see Section C.2.4, Pipetting Speed Adjustment).
*NOTE: IMPACT2 speed settings are changed through the Special Function key (see Special Function key section on page 11) or by using the volume key (see Section C.2.4, Pipetting Speed Adjustment).
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PIPETTOR OVERV IEW
[Prog]
IMPACT
only
[Purge]
The current speed setting is always shown at the bottom of the display. These settings can be selected and changed at any time before a program step is performed.
The Program key on the IMPACT2 enables you to store up to five separate programs in memory for
2
future recall. Each program can hold up to 40 steps. Programs are stored in reserved program location numbers 1 – 5. Program “0” is used as a “scratch pad” where temporary programs can be written, run, and overwritten easily. Program “0” is considered to be an “open” program (see Section C.2, Program­ming the IMPACT2).
The Purge key enters a purge prompt anytime during a pipetting operation to abort the program. By pressing the Purge key, then pressing the trigger, you can dispense any remaining liquid from the pipet tips and return to the beginning of the program.
The Purge key is also used to enter the purge step into a program.
Note: If there is insufficient liquid for a final dispense step, the pipettor emits a beep tone and auto­matically displays the purge prompt.
RUN The RUN mode performs the operation that is
displayed, such as fill, dispense, mix, or purge. When "Run" appears in the display, you can activate the RUN mode by pressing the trigger. At the end of a pipetting sequence, the pipettor emits a triple beep tone and returns to the beginning of the program. The pipettor remains in RUN mode until you program a new pipetting sequence.
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Program Keys:
PIPETTOR OVERVIEW
[Fill]
[Disp]
[Mix]
s
Vol
t
The Fill, Dispense, and Mix keys program the pipetting operations. By pressing one of these keys, you can enter the programming mode of the IMPACT. The "Run" prompt disappears from the display and the appropriate operation appears with a flashing "Vol" prompt. Fill, dispense, or mix volumes can be programmed by pressing the Volume key. Also, the number of mix cycles can be programmed by pressing the Mix key twice.
Automatic blowout. At the end of a final dispense step, the pipettor automatically expels any remaining liquid from the pipet tips with a short burst of air (blowout). The pistons then reverse direction and return to zero position.
Note: If you press and hold in the trigger during the final dispense step, the pipettor performs the automatic blowout and holds its position until you release the trigger. This delay allows you sufficient time to remove the tips from the liquid to prevent aspiration of liquid, as the pistons return to zero position.
The Volume key controls the quantity of liquid to be aspirated or dispensed. The volume can be entered when the "Vol" prompt appears.
IMPACT
only
Pipetting volume can be incremented quickly by continuously depressing the Volume key. Short, quick, depressions of the Volume key will increase or decrease the volume by its smallest increment. Longer depressions of the Volume key will increase or decrease the volume in larger increments. Three levels of volume increments are available to achieve the desired volume (see Table C.1, Default Program­ming Volumes).
The Volume key can also be used to change
2
pipetting speeds within a stored program or during the execution of a program (see Section C.2.4, Pipetting Speed Adjustment).
9
PIPETTOR OVERVIEW
[Enter]
The Enter key stores the program in memory. After each step is programmed, the Enter key is pressed once to accept the step. After the final step is programmed, the Enter key is pressed twice to denote the end of the program. The pipettor verifies the end of the program with a triple beep tone.
Special Function Key:
The Special Function key is the small hole below the Volume key. It can be activated with a pipet tip. By repeatedly pressing the Special Function key, you can display four functions: Beep Tone (TONE), Paced Dispense Speed (PACE), Calibration (CAL), and Pipetting Speed—IMPACT2 only (SPd). To exit the Special Function key at any time, press the Enter key.
TONE The beep tone signals the completion of one or more
steps, or signals a warning:
n Single beep tone: end of pipetting step; insufficient
dispense volume, purge required.
n Double beep tone: operation or program key is not
active in current mode; illegal programming step attempted. See Section E.1, Troubleshooting.
n Triple beep tone: end of pipetting sequence;
end of programming sequence.
The single beep tone, signaling the end of a pipetting step, can be turned ON or OFF. All other beep tones cannot be turned off. Press the Special Function key once to display "TONE" and its current setting. To change the setting, press the Volume key. If the beep tone is turned ON, the beep tone symbol " " appears at the bottom of the display.
Note: For paced dispensing, the beep tone should be turned on, to prepare you for each dispense step.
PACE The pace controls the speed of repeat pipetting
(paced dispensing), FAST or SLOW. Press the Special Function key twice to display "PACE" and its current setting. To change the setting, press the Volume key.
10
PIPETTOR OVERVIEW
Paced Dispense. When you use the paced
dispense feature, the pipettor dispenses the programmed volume at the specified pace (fast or slow). Immediately after each dispense step, the pipettor emits a single beep tone (if beep tone is on) to prepare you for the next dispense step. See Section C.5, Operating Procedures.
CAL The calibration of the pipettor is factory set for
distilled water at room temperature. If you are pipetting fluids with significantly different specific gravities or temperatures, use this setting to recalibrate the pipettor. See Section D, Calibration.
SPd
IMPACT
only
Once in the special function mode, adjust the pipetting speed by pressing the Volume key. Five
2
speed settings are available: Speed Setting Display
SLOW S MEDIUM SLOW S MEDIUM S MEDIUM FAST S FAST S F
The current speed setting is always shown at the bottom of the display. These settings can be selected and changed at any time before a program step is performed.
11
PIPETTOR OVERVIEW
Low Battery Indicator
Program No.
B.3 Display
The pipettor display is a dynamic LCD screen. It displays the current operation, volume prompt (if you are entering a program), pipetting volume, low battery indicator (appears if battery charge is low), beep tone symbol (if the beep tone is on), pipetting speed, and program number (IMPACT2 only).
Pipetting Operations
Volume Prompt Pipetting Volume
Beep Tone Speed Setting Purge Prompt
Low Battery Indicator
Figure B.3a: IMPACT Display
Pipetting Operations
Beep Tone
Volume Prompt
Speed Setting Purge Prompt
Pipetting Volume
Figure B.3b: IMPACT2 Display
12
B.4 Specifications
2.5%/0.15 µl
2.5%/0.15 µl
Pipettor Type
Model Handle Color IMPACT White
IMPACT2
Handle Specifications
PIPETTOR OVERVIEW
Gray
Handle
Size
12.50 µl 12-channel 0.50-12.50
125.0 µl 12-channel 2.0-125.0 µl Yellow 2.0%/1.0 µl 1.2%/0.8 µl
250.0 µl 12-channel 5.0-250.0 µl Blue 2.0%/1.5 µl 0.8%/1.2 µl 850 µl 12-channel 15-850 µl Orange 1.5%/4.0 µl 0.6%/2.5 µl
12.50 µl 8-channel 0.50-12.50
125.0 µl 8-channel 2.0-125.0 µl Yellow 2.0%/1.0 µl 1.0%/0.6 µl
250.0 µl 8-channel 5.0-250.0 µl Blue 2.0%/1.5 µl 0.7%/1.0 µl
1250 µl 8-channel 15-1250 µl Green 1.5%/6.0 µl 0.6%/3.0 µl
Handle Type Volume
Range
µl
µl
Color
Ring
Red
Red
Accuracy*
(±)
Precision**
(±)
2.0%/0.15 µl
2.0%/0.15 µl
Note: For Accuracy and Precision, values shown are expressed as a percent (%) deviation or microliter (µl) value. When applied to desired volume, the greater of the two values will always apply.
*Both values represent the deviation from the mean. **Percentage values are expressed as the coefficient of variation.
Microliter values are expressed as the standard deviation.
Available Power Sources
Catalog No. Country Power Source
6096 England 240 V, 50 Hz 6097 Japan 100 V, 50 Hz
13
PIPETTOR OVERVIEW
6098 USA 120 V, 60 Hz 6099 Europe 230 V, 50 Hz
14
PIPETTOR OVERVIEW
B.5 Hazards and Precautions
There are no known hazards associated with the IMPACT when it is operated in accordance with the instructions in this manual. However, you should be aware of situations that can result in serious injury.
Note: Do not perform troubleshooting procedures on the internal components unless instructed by Matrix Technologies service personnel.
WARNING! Ensure that the power transformer is connected to a power receptacle that provides voltage and current specified by Matrix Technologies. Use of an incompatible power receptacle can cause shock and fire hazard.
CAUTION! Use only the power transformer supplied by Matrix Technologies. Use of an incompatible power transformer can damage the pipettor.
CAUTION! Always turn off the power and unplug the power transformer before cleaning the exterior. Fluid seepage can damage internal components.
CAUTION! Do not immerse the unit in liquid. Fluid seepage can damage internal components.
CAUTION! Do not autoclave the entire unit. Extreme heat can damage the display and other electronic components.
Note: For autoclaving specific portions of the pipettor, refer to the Autoclaving Procedures, or call Matrix Technologies, Technical Service Department, for instructions.
CAUTION! Do not clean the keypad with bleach solution or other solvents. Caustic cleaning solutions can damage the keypad.
CAUTION! Avoid excessive charging of the batteries when the pipettor is not in use. Excessive charge to the batteries will shorten the battery life and may damage the batteries.
15
C. PROGRAMMING AND OPERATION
Programming the IMPACT and IMPACT2 is fast and easy. Pipetting steps and volume are entered in the sequence that they will be performed, then the program is saved to memory. This program will stay in memory (even if the pipettor is turned off, or the battery runs low) until you change it. Up to 40 programming steps can be entered and stored in the IMPACT pipettor. Five programs, each capable of holding 40 steps, can be entered and stored in the
IMPACT
C.1 Programming the IMPACT
To enter a pipetting program:
1. Turn on pipettor. Press the green [On/Off] key to turn on
2
.
the pipettor. The pipettor will beep once and flash "ZERO" on the display while resetting the pistons to zero position. The first step of the current program is displayed.
Note: If the pipettor is being programmed for the first time, the first step will be the fill operation, with the default fill volume displayed (see the following table).
Handle
Volume
12.50 10.00 10.00 5.00 0.05 0.10 0.50
125.0 100.0 100.0 50.0 0.1 0.5 5.0
250.0 250.0 50.0 50.0 0.5 5.0 25.0 850 850 100 100 1 5 50
1250 1250 100 100 1 5 50
Fill
Volume
Dispense
Volume
Mix
Volume
Volume Increment
(slow –> fast)
Table C.1: Default Programming Volumes*
*All volumes shown are in microliters (µl).
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