LW Scientific Digital ZipSpin User Manual

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24 Volt Digital ZipSpin24 Volt Digital ZipSpin
Instruction Manual
Model pictured:
Digital ZipSpin Centrifuge
G
A
B
A Lid B Rotor C Timer Knob D Speed Knob E Display F Release Button G Safety Switch H ON/OFF Switch I Non-Slip Rubber Base J Power Socket
C
H
J
F
E
D
I
The LW Scientific Digital ZipSpin is loaded with user-friendly features such as digital read out of time and speed, digital calibration, imbalance detection, and last cycle run recall. Designed with robust construction for long life, the Digital ZipSpin is well-suited to accomplish blood and urine separations, micro filtrations, and DNA preparations. Since more and more tests are done with smaller sample sizes, this design includes two interchangeable rotors: an 8-place closed rotor for
1.5/2.0ml micro-tubes and a PCR strip rotor (16 places) for 0.2ml micro-tubes. Also included are 2 sets of adapters for
0.4/0.5ml micro-tubes and 0.2ml micro-tubes. The small footprint and quiet operation make it the perfect addition to any clinic or lab.
Please read and complete the warranty form online at LWScientific.com/warranty_form. The warranty form documents your purchase. Failure to fill out the warranty form may void any warranty claims on the unit.
P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046
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Installation
On/Off Switch
The Digital ZipSpin Centrifuge is supplied in a Box. Open the Box, then carefully remove centrifuge from the Box and connect the electrical cable. The instruction manual and the accessories should be kept with the centrifuge. Please keep all packaging in safe storage for at least 1 year for warranty purposes.
Make sure the rotor is tight enough to avoid any accident. Load the rotor with Micro tubes for centrifugation. Flip on the switch on the rear, set the desired speed and time and close the lid to start centrifugation.
Power Socket
Operation
Switch ‘On’ the centrifuge from rear side so that display will show you the readings.
Note : ‘En’ Symbol indicates that Imbalance detection feature is enabled.
Load the rotor symmetrically to avoid accident and damage of centrifuge.
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Close the lid to start centrifugation. Use the Speed knob to set the speed and the Timer knob to set the run time.
2
Once the centrifugation is completed, turn speed knob or use timer knob to set the display value to zero. This will
3
gradually stop the rotor.
If you want to stop the centrifuge quickly, push the lid release button and the rotor will stop within 2 seconds.
4
After the rotor has stopped, press the lid release button and open lid with your thumb in front and fingers on the top -
5
simply lift the lid back on the hinge.
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Balancing Rotor
To spin 8 samples, fill 8 test tubes equally. Then insert them into the holes.
To spin 4 samples, fill the 4 test tubes equally. Then insert them into alternate holes of the rotor.
To spin 3 samples, fill the 3 test tubes equally. Next insert tubes in alternate holes of the rotor. Now fill a test tube with water and insert into a hole for balancing the rotor
To spin 2 samples, fill 2 test tubes equally. Insert them into the holes of rotor in opposite one another.
Positions can be 1-5, 2-6, 3-7, 4-8.
To spin 1 sample, fill a test tube with sample then insert into any hole of the rotor. Now insert 1 test tube filled with water opposite of the sample.
Positions can be 1-5, 2-6, 3-7, 4-8.
To spin individual tubes, make sure they are balanced opposite each other as shown in diagram. (1-2, 3-4, etc.)
IMPORTANT:
8 different types of test tubes can be spun by
using the two interchangeable rotors.
P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046
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Interchangeable Rotor
8 x 0.2 ,0.4 ,0.5 ,1.5 ,2 ml
Changing the Rotor
8-Place Rotor
Rotor
Set Screw
Motor Shaft
PCR Rotor
16 x 0.2 ml
Wrench
Insert the L-arm hex wrench into the marked hole on the side of the rotor. The grub screw is located at the bottom of the
1
rotor on the marked side.
To remove the rotor, turn counter-clockwise to loosen the screw. Gently pull the rotor up.
2
Insert the new rotor from the top onto motor shaft and tighten the screw again. Turn clockwise to tighten screw, which
3
is located under the bottom of rotor.
Please make certain the rotor is tight enough to avoid any accident.
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Time & Speed Settings and Display
Infinite Time Display
Time Indicator Display
This unit is equipped with a 0-25 minute timer. Turn the timer knob until the desired time is displayed. The time increments will be displayed in minutes.
The timer can be set from 00 to 25 minutes. If a cycle longer than 25 minutes is desired, turn past 25 for continuous operation.
The timer is a count down timer and can be reset or adjusted in between the runs. The centrifuge will stop when the timer reaches “00.”
P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30045
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Time & Speed Settings and Display Cont.
Speed Indicator Time Indicator
During the operation of centrifuge, the time and speed will be displayed alternatively accompanied by speed indicator and time indicator respectively.
Quick spin function: By keeping the timer to position and speed set to any desired setting, one can open and close the lid to operate the centrifuge in quick spin function mode.
Speed control: Speed is set by rotating the speed control knob. While adjusting the speed, the speed indicator will illuminate. The minimum speed is 100rpm. Actual rpm can be determined by multiplying the number in the display by
100.
RCF value: Based on the speed and rotor the actual RCF value will vary. Please refer to the table given on the last page of this booklet to get the actual RCF of different rotors at certain given speed values.
Imbalance Detection
When centrifuge is turned on, one of the following will display:
• ‘En’: Imbalance detection is activated
• ‘d5’: Imbalance detection is de-activated
The imbalance detection feature can be activated or deactivated by pressing and holding the timer knob.
Operating the centrifuge with an unbalanced load may cause excessive vibration and damage the centrifuge. When the imbalance detector is activated, and an unbalanced load is spun, the centrifuge will shut down, as indicated by flashing display: ‘Ub
USER ACTION: ‘Ub
Open the lid, correct the unbalanced load. Close the lid and `reset the centrifuge’ by turning unit `Off’ and ‘On’ using the On/Off switch located on the rear side of the centrifuge.
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Calibration
Your centrifuge comes factory-calibrated for accurate speeds with the 8-place tube rotor. This is the default setting. When installing the lighter PCR rotor, you may need to follow these instructions for more accurate speeds.
Switch `On’ the centrifuge from rear side. Press and hold the speed knob to allow the centrifuge to go into calibration mode. CL will be indicated on display. Run the centrifuge at the preset speed and check the rpm of rotor with an external tachometer. If there is any difference in the set speed and tachometer reading, adjust the speed by rotating the speed knob to match the set speed with tachometer reading. Once the rpm is set, gently press and hold the speed knob again to save the settings. Calibration will be valid for the
current cycle only.
Care and Maintenance
Your centrifuge and its rotor should be cleaned periodically. Ensure that while cleaning the unit is not plugged in. To clean the centrifuge, use a damp cloth and a mild, noncorrosive detergent (ph < 8). Do not immerse the centrifuge in liquid or pour liquids over it. Excessive amounts of liquid should be avoided, Liquid should not come into contact with the motor. After cleaning, ensure all parts are dried thoroughly before attempting to operate the unit.
Should a spill of infectious materials occur within the rotor or rotor chamber, the unit should be disinfected. This should be performed by qualified personnel with proper protective equipment.
Because of the safety issues with high g-forces in a centrifuge, it is recommended that rotors, buckets, and tube sleeves be inspected every 6 months for corrosion and fatigue. If there is any indication of wear, the rotor, buckets, and sleeves should be removed from service. Contact LW Scientific for return instructions, so the rotor can be evaluated by an LW Scientific technician for repair or replacement. It is also recommended that after 2 years of service rotors, buckets, and sleeves be returned to LW Scientic for inspection. Following these procedures will ensure safety of lab personnel as well as extend the life of the centrifuge.
P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046
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Operating Conditions
The Digital ZipSpin centrifuge is designed to be safe under the following conditions when operated with necessary precautions:
• For indoor use only.
• Ambient temperature of 4°C to 65°C.
• Main supply voltage fluctuations not to exceed +/- 10% of the nominal voltage.
• Position at least 20 cm from walls, 20 cm from ceiling and 20 cm between other products.
• Make sure to always install the centrifuge on stable flat surfaces only & place it near the electrical outlet.
• Make sure to always load the rotor symmetrically every time. Each tube should be counterbalanced by another tube.
• Make sure to always use only specified micro centrifuge tubes made from plastic and designed to withstand centrifugal forces of at least 2000 g.
• Do not use the centrifuge in any manner not specified in these instructions.
• Do not operate the centrifuge without the rotor being properly attached to the shaft. Re-check by trying to pull it up manually, especially after rotor assembly.
• Do not move the centrifuge while the rotor is spinning.
• Do not fill tubes while they are in the rotor, liquid spillage may harm the unit.
• Do not put hands in the rotor unless the rotor has completely stopped.
• Do not centrifuge flammable, explosive or corrosive materials.
• Do not use solvents or flammables near this or other electrical equipment.
• In case of the unit dropping from the table please check the casing for any cracks/damage.
• The centrifuge and its components are non-autoclavable.
• Speeds for tube rotor are accurate to +/- 5%
• Speeds for PCR rotor are accurate to +/- 12%
RCF CHART
RPM ROUND ROTOR PCR STRIP
1000
2000
3000
4000
4500
5000
6000
0.2ml
35.5
142
319
567
718
886
1276
0.5ml
47
188
424
753
953
1177
1695
1.5ml
52
208
468
832
1053
1300
1872
0.2ml
54
217
488
868
1098
1356
1952
0.2ml
52
208
467
830
1050
1297
1868
P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046
Fluid Recommendations:
Whole Blood: 6,000 rpm 10 mins. Urine: 2,800 rpm 5-10 mins.
MKT-7.5.3-L-183 | Rev 0
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