Luminex 200 User Manual
Lum/nex
®
Luminex 200
LDS Version 1.7
™
User Manual for
© LUMINEX CORPORATION, 1998 - 2005. All rights reserved. No part of this publication may be
reproduced, transmitted, transcribed, or translated into any language or computer language, in any form or
by any means without prior express, written consent of:
LUMINEX CORPORATION
12212 Technology Boulevard
Austin, Texas 78727-61 15
U.S.A.
V oice: (512) 219-8020
Fax: (512) 219-5195
Technical Support U.S. and Canada: toll-free 1-877-785-2323
Technical Support international: +1-512-381-4397
Technical Support fax: 512-219-5195
Luminex® 200™ User Manual for LDS Version 1.7
CN-M078-01
PN 89-00002-00-150 R e v. A
October , 2005
Luminex reserves the right to modify its products and services at any time. This guide is subject to change
without notice. Although prepared to ensure accuracy, Luminex assumes no liability for errors or omissions,
or for any damages resulting from the application or use of this information.
The following are trademarks of Luminex Corporation: Luminex, Luminex 100, Luminex HTS,
Luminex 100 IS, Luminex 200, LabMAP, LumAvidin, Luminex XYP, Luminex SD, xMAP, and FlexMAP. All
other trademarks, including Windows, Cheminert, Tween, Pentium, Dell, Alexa and Titertube
trademarks of their respective companies.
The contents of this manual and the associated Luminex soft ware are the proper ty of LUMINEX
CORPORATION and are copyrighted. Any reproduction in whole or in part is strictly prohibited.
are registered
Standard Terms and Conditions For Use of Product
By opening the packaging containing this product ("Product") or by using such Product in any manner,
you are consenting and agreeing to be bound by the following terms and conditions. You are also agreeing that the following terms and conditions constitute a legally valid and binding contract that is enforceable against you. If you do not agree to all of the terms and conditions set forth below, you must promptly
return the Product for a full refund prior to using them in any manner.
1. Acceptance - ALL SALES ARE SUBJECT TO AND EXPRESSLY CONDITIONED UPON THE
TERMS AND CONDITIONS CONTAINED HEREIN, AND UPON BUYER'S ASSENT
THERETO. NO VARIATION OF THESE TERMS AND CONDITIONS SHALL BE BINDING
UPON LUMINEX CORPORATION ("LUMINEX") UNLESS AGREED TO IN WRITING AND
SIGNED BY AN AUTHORIZED REPRESENTATIVE OF LUMINEX. For purposes of this agreement, "Seller" shall mean the Luminex authorized reseller that sells the Product to Buyer. Buyer, by
accepting the Product shall be deemed to have assented to the terms and conditions set forth herein,
notwithstanding any terms contained in any prior or later communications from Buyer and whether or
not Seller shall specifically or expressly object to any such terms.
2. Warranties - Any warranty obligations for the Product shall be exclusively provided in writing to
Buyer directly by Seller. LUMINEX MAKES NO WARRANTY WHATSOEVER REGARDING
THE PRODUCT AND LUMINEX SPEFICALLY DISCLAIMS ALL WARRANTIES, EXPRESS
OR IMPLIED, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. NEITHER SELLER NOR LUMINEX SHALL IN ANY
EVENT BE LIABLE FOR INCIDENT AL, CONSEQUENTIAL OR SPECIAL DAMAGES OF ANY
KIND RESULTING FROM ANY USE OR FAILURE OF THE PRODUCT, EVEN IF SELLER OR
LUMINEX HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGE INCLUDING,
WITHOUT LIMITATION, LIABILITY FOR LOSS OF WORK IN PROGRESS, DOWN TIME,
LOSS OF REVENUE OR PROFITS, FAILURE TO REALIZE SAVINGS, LOSS OF PRODUCTS
OF BUYER OR OTHER USE OR ANY LIABILITY OF BUYER TO A THIRD PARTY ON
ACCOUNT OF SUCH LOSS, OR FOR ANY LABOR OR ANY OTHER EXPENSE, DAMAGE OR
LOSS OCCASIONED BY SUCH PRODUCT INCLUDING PERSONAL INJUR Y OR PROPERTY
DAMAGE UNLESS SUCH PERSONAL INJURY OR PROPERTY DAMAGE IS CAUSED BY
SELLER'S GROSS NEGLIGENCE.
3. Buyer's Use of Product -Buyer agrees that no rights or licenses under Luminex's patents shall be
implied from the sale of the Product, except as expressly provided herein, and Buyer does not receive
any right under Luminex's patent rights hereunder. Buyer acknowledges and agrees that the Product
is sold and licensed only for use with Luminex's standard fluorescently dyed microspheres. Buyer
further acknowledges that the Product have not received approval from the United States Food and
Drug Administration or other federal, state or local regulatory agencies and have not been tested by
Seller or Luminex for safety or efficacy in food, drug, medical device, cosmetic, commercial or any
other use, unless otherwise stated in Seller's technical specifications or material data sheets furnished
to Buyer. Buyer expressly represents and warrants to Seller that Buyer will properly test and use any
Product in accordance with the practices of a reasonable person who is an expert in the field and in
strict compliance with the United States Food and Drug Administration and all applicable domestic
and international laws and regulations, now and hereinafter enacted.
BUYER HEREBY GRANTS TO LUMINEX A NONEXCLUSIVE, WORLDWIDE, UNRE-
STRICTED, ROYALTY-FREE, FULLY PAID-UP LICENSE, WITH THE RIGHT TO GRANT AND
AUTHORIZE SUBLICENSES, UNDER ANY AND ALL PATENT RIGHTS IN INVENTIONS
COMPRISING MODIFICATIONS, EXTENSIONS, OR ENHANCEMENTS MADE BY BUYER
TO THE PRODUCT OR TO THE MANUFACTURE OR USE OF THE PRODUCT ("IMPROVEMENT PATENTS"), TO MAKE, HAVE MADE, USE, IMPORT, OFFER FOR SALE OR SELL
ANY AND ALL PRODUCT; EXPLOIT ANY AND ALL METHODS OR PROCESSES; AND
OTHERWISE EXPLOIT IMPROVEMENT PATENTS FOR ALL PURPOSES. NOTWITHSTANDING THE FOREGOING, "IMPROVEMENT PATENTS" SPECIFICALLY EXCLUDES PATENT
CLAIMS CONCEIVED AND REDUCED TO PRACTICE BY BUYER CONSISTING OF METHODS OF SAMPLE PREPARA TION, METHODS OF CONJUGATING PRODUCT TO ANALYTES,
THE COMPOSITION OF MATTER OF THE SPECIFIC CHEMISTRIES OF THE ASSAYS
DEVELOPED BY BUYER AND METHODS OF PERFORMING THE ASSAYS (I.E., THE PROTOCOL FOR THE ASSAY).
Buyer has the responsibility and hereby expressly assumes the risk to verify the hazards and to conduct any further research necessary to learn the hazards involved in using the Product. Buyer also has
the duty to warn Buyer's customers, employees, agents, assigns, officers, successors and any auxiliary
or third party personnel (such as freight handlers, etc.) of any and all risks involved in using or handling the Product. Buyer agrees to comply with instructions, if any, furnished by Seller or Luminex
relating to the use of the Product and not misuse the Product in any manner. Buyer shall not reverse
engineer, decompile, disassemble or modify the Product. Buyer acknowledges that Luminex retains
ownership of all patents, trademarks, trade secrets and other proprietary rights relating to or residing
in the Product.
4. Buyer's Representations, Release and Indemnity - Buyer represents and warrants that it shall use
the Product in accordance with Paragraph 2, "Buyer's Use of Product," and that any such use of Product will not violate any law, regulation, judicial order or injunction. Buyer agrees to release, discharge, disclaim and renounce any and all claims, demands, actions, causes of action and/or suits in
law or equity, now existing or hereafter arising, whether known or unknown, against Seller and
Luminex, and their respective officers, directors, employees, agents, successors and assigns (collectively the "Released Parties"), with respect to the use of the Product. Buyer agrees to indemnify and
hold harmless the Released Parties from and against any suits, losses, claims, demands, liabilities,
costs and expenses (including attorney, accounting, expert witness, and consulting fees) that any of
the Released Parties may sustain or incur as a result of any claim against such Released Party based
upon negligence, breach of warranty, strict liability in tort, contract or any other theory of law or
equity arising out of, directly or indirectly, the use of the Product or by reason of Buyer's failure to
perform its obligations contained herein. Buyer shall fully cooperate with the Released Parties in the
investigation and determination of the cause of any accident involving the Product which results in
personal injury or property damage and shall make available to the Released Parties all statements,
reports, recordings and tests made by Buyer or made available to Buyer by others.
5. Patent Disclaimer - Neither Seller nor Luminex warrants that the use or sale of the Product will not
infringe the claims of any United States or other patents covering the product itself or the use thereof
in combination with other products or in the operation of any process.
End-User License Agreement (EULA) for Luminex® Software
This Luminex End-User License Agreement (“EULA”) is a legal agreement between you (either an individual
or a single entity , also referred herein as “you”) the end-user and Luminex Corporation (“Luminex”) regarding
the use of the Luminex software product identified above, which includes computer softwar e and online or
electronic documentation and may include associated media and printed materials (if any) (“SOFTWARE
PRODUCT” or “SOFTWARE”).
The SOFTWARE PRODUCT is protected by copyright laws and international copyright treaties, as well as
other intellectual property laws and treaties. The SOFTWARE PRODUCT is licensed, not sold.
1. GRANT OF LICENSE. Subject to the terms and conditions of this EULA, Luminex hereby grants to you a
nonexclusive, nontransferable, nonassignable license (without right to sublicense) under Luminex’s
copyrights and trade secrets to use the SOFTWARE PRODUCT on a hardwar e platform purchased from
Luminex pursuant to Luminex’s terms and conditions of sale. You may make one (1) copy of the
SOFTWARE PRODUCT for backup or archival purposes only. Although no rights or licenses under any
of Luminex's patents are granted by or shall be implied from the license of the SOFTW ARE or the sal e of
Luminex instrumentation to you, the purchaser, you may obt ain a license under Luminex’s patent s, if any ,
to use this unit of Luminex instrumentation with fluorescently labeled microsphere beads authorized by
Luminex by purchasing such microspheres from Luminex or an authorized Luminex reseller.
2. RESTRICTIONS.
• You must maintain all proprietary notices on all copies of the SOFTWAR E PRODUC T.
• You may not distribute copies of the SOFTWARE PRODUCT to third parties.
• You may not reverse-engineer, decompile, disassemble, or otherwise attempt to derive source
code from the SOFTWARE PRODUCT.
• You may not copy (other than one backup or archival copy), distribute, sublicense, rent, lease,
transfer or grant any rights in or to all or any portion of the SOFTWARE PRODUCT.
• You must comply with all applicable laws regarding the use of the SOFTWARE PRODUCT.
• You may not modify or prepare derivative works of the SOFTWARE PRODUCT.
• You may not use the SOFTWARE PRODUCT in a computer-based service business or publicly
display visual output of the SOFTWARE PRODUCT.
• You may not transmit the SOFTWARE PRODUCT over a network, by telephone, or
electronically by any means.
3. TERM AND TERMINA TION. Your rights under this EULA are effective until termination. You may
terminate this EULA at any time by destroying the SOFTWARE PRODUCT, including all computer
programs and documentation, and erasing any copies residing on your computer equipment. Luminex
may terminate this EULA upon thirty (30) days written notice to you. Your rights under this EULA
automatically terminate without further action on the part of Luminex if you do not comply with any of the
terms or conditions of this EULA. Upon any termination of this EULA, you agree to destroy the
SOFTWARE PRODUCT and erase any copies residing on your computer equipment.
4. RIGHTS IN SOFTWARE. All rights and title in and to the SOFTWARE PRODUCT and any copies
thereof are owned by Luminex or its suppliers. This EULA is not a sale and does not transfer to you any
title or ownership interest in or to the SOFTWARE or any p atent, copyright, trade secret, trade n ame,
trademark or other intellectual property right therein. You shall not remove, alter, or obscure any
proprietary notices contained on or within the SOFTWARE and shall reproduce such notices on any
back-up copy of the SOFTWARE. All title and intellectual property rights in and to the content which may
be accessed through use of the SOFTWARE PRODUCT is the property of the respective content owner
and may be protected by applicable copyright or other intellectual property laws and treaties. This EULA
grants you no rights to use such content.
5. EXPORT RESTRICTIONS. You agree that you will not export or re-export the SOFTWARE PRODUCT
to any country , person, entity, or end-user subject to U.S.A. export restrictions. You hereby warrant no
state or federal agency has suspended, revoked, or denied your export privileges.
6. NO WARRANTY. THE SOFTW ARE PRODUCT IS LICENSED “AS IS.” ANY U SE OF THE SOFTW ARE
PRODUCT IS A T YOUR OWN RISK. THE SOFTWARE PRODUCT IS PROVIDED FOR USE ONLY
WITH LUMINEX PRODUCTS. TO THE MAXIMUM EXTENT PERMITTED BY APPLICABLE LAW,
LUMINEX AND ITS SUPPLIERS DISC LAI M ALL WARRANTIES, EITHE R E XPRE SS OR I MPL IED,
INCLUDING, BUT NOT LIMITED TO, IMPLIED W ARRANTIES OF MERCHANT ABILITY, FITNESS FOR
A PARTICULAR PURPOSE, AND NON INFRI NG EMENT.
7. LIMIT ATION OF LIABILITY. IN NO EVENT SHALL LUMINEX OR ITS SUPPLIERS BE LIABLE FOR
ANY SPECIAL, INCIDENTAL, INDIRECT, OR CONSEQUENTIAL DAMAGES WHA TS OEVER
(INCLUDING, WITHOUT LIMITATION, DAMAGES FOR LOSS OF BUSINESS PROFITS, BUSINESS
INTERRUPTION, LOSS OF BUSINESS INFORMA TION, OR ANY OTHER PECUNIARY LOSS)
ARISING OUT OF THE USE OF OR INABILITY TO USE THE SOFTWARE PRODUCT, EVEN IF
LUMINEX HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES.
MISCELLANEOUS. This EULA is governed by the laws of the State of Texas, U.S.A., without reference to
conflicts of laws principles. You shall not assign or sublicense or otherwise transfer the rights or license
granted hereunder, by agreement or by operation of law, without the prior written consent of Luminex, and all
assignments in violation of this prohibition shall be null and void. This EULA is the complete and exclusive
agreement of Luminex and you and supersedes all other communications, oral or written, relating to the subject matter hereof. No change to this EULA shall be valid unless in writing and signed by the party against
whom enforcement is sought. The waiver or failure of Luminex or you to exercise in any respect any right or
rights provided for herein shall not be deemed a waiver of any further right hereunder. If any provision of this
EULA is held unenforceable, the remainder of this EULA will continue in full force and effect.
EULA PN: 89-30000-00-070
Contents
Introduction 1-1
Principle of Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Basic Concepts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Excitation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
xMAP Microspheres. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Reporter Fluorochromes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Fluorescence Compensation . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Repetitive Microsphere Measurements . . . . . . . . . . . . . . . . . .1-4
About this Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Technical Assistance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Safety 2-1
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Safety and Regulatory Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Warnings and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Electrical. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
Mechanical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7
Biological . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7
Heat. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8
Blue Indicator Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8
Equipment Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8
System Overview 3-1
Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
xMAP Reagents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Required Laboratory Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Luminex 200 Performance Specifications . . . . . . . . . . . . . . . . . . .3-2
Speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Accuracy and Precision . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Sensitivity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Luminex 200 Analyzer Specifications . . . . . . . . . . . . . . . . . . . . . .3-3
Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Electronics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
PN 89-00002-00-150 Rev. A i
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
Luminex XYP Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Luminex SD System General. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
PC Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Recommended Additional Equipment . . . . . . . . . . . . . . . . . . . . . . 3-5
Printer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Uninterruptible power supply (UPS). . . . . . . . . . . . . . . . . . . . 3-5
Surge Protector. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Vortex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Bath Sonicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Luminex System Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Electronic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Optical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
Luminex XYP Reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
Luminex XYP Heater Block . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
Installation 4-1
Luminex 200 System Installation. . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Luminex 200 Software Setup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Adjusting the Sample Probe Height. . . . . . . . . . . . . . . . . . . . . . . . 4-4
Installing Software and Firmware . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
What to Do Next. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
System Startup 5-1
Luminex Main Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Add New Classification Channel Calibration Lots . . . . . . . . . 5-2
Add New Reporter Channel Calibration Lots . . . . . . . . . . . . . 5-4
Calibrate the Luminex Analyzer . . . . . . . . . . . . . . . . . . . . . . . 5-5
Preview or Print Calibration Report . . . . . . . . . . . . . . . . . . . . 5-7
Analyzer menu commands. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-9
Data Acquisition 6-1
Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
User tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
Acquisition tab. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3
Advanced tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-3
Info tab. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4
Setup XY Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4
Sessions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5
Create a Session Using Default Settings. . . . . . . . . . . . . . . . . 6-6
Create a Session Using a Template . . . . . . . . . . . . . . . . . . . . . 6-6
Open and Add to an Existing Session . . . . . . . . . . . . . . . . . . . 6-8
Open an Existing Session and Save it as a New Session. . . . . 6-9
Templates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-9
Create a Template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-9
Modify a Template. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-9
ii PN 89-00002-00-150 Rev. A
xMAP Technology Contents
Delete a Template. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-10
Multiplex Acquisition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-10
Menu Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-10
Toolbar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-12
Main Control and Session Detail . . . . . . . . . . . . . . . . . . . . . .6-12
Right-Click Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-14
Session Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-14
Histogram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-17
Gates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-18
Analyzer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-19
Bar Graph/Dot Plot Display . . . . . . . . . . . . . . . . . . . . . . . . . .6-20
System Monitor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-22
Status Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-24
Data Collection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-24
Run an Entire Plate of Samples . . . . . . . . . . . . . . . . . . . . . . .6-25
Run a Range of Samples. . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-26
Run a Single Sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-27
Interrupt a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-27
Reports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-28
Maintenance and Cleaning 7-1
Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2
Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2
Check Fluid Levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Check for Leaks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 -3
Calibrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Sanitize . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Clean Accessible Surfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-4
Clean Sample Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-4
Replace Air Intake Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-5
Replace Syringe Seal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-6
Clean Luminex Analyzer Ventilation Filter . . . . . . . . . . . . . . . . . .7-7
Change Sheath Filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-7
Change Sheath Quick-disconnect O-rings . . . . . . . . . . . . . . . . . . .7-8
Change Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-9
Adjust Sample Probe Vertical Height. . . . . . . . . . . . . . . . . . . . . . .7-9
Storing the Analyzer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-10
Decontaminating the Luminex Analyzer for Return Shipment. . .7-11
Troubleshooting 8-1
Power Supply Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2
Communication. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2
Pressurization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3
Fluid Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-4
Sample Probe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-5
Calibration Problems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-7
Acquisition Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-9
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Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
Bead Detail Irregularities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-11
Error Messages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-13
Printing Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-14
Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-14
Protocols 9-1
xMAP Microsphere Handling . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
Microsphere Dispersion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
Probe Sonicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2
Bath Sonicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2
Enumeration of xMAP Microsphere Suspensions. . . . . . . . . . 9-3
xMAP Microsphere Separation Methods. . . . . . . . . . . . . . . . . 9-3
xMAP Microsphere Agitation During Assay . . . . . . . . . . . . . 9-3
xMAP Microsphere Stability and Storage. . . . . . . . . . . . . . . . 9-4
Two-Step Carbodiimide Coupling of Protein to xMAP
Carboxylated Microspheres . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-4
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-4
Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-4
Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-4
Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-5
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-5
Technical Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-6
One-Step Carbodiimide Coupling of
Oligonucleotides to xMAP Carboxylated Microspheres . . . . . . . . 9-7
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-7
Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-7
Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-7
Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Technical notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-9
Binding Biotin-conjugated Molecules to
LumAvidin-Modified xMAP Microspheres. . . . . . . . . . . . . . . . . . 9-9
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-9
Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-9
Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-10
Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-10
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-10
Technical notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-11
Glossary A-1
Product Numbers B-1
Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1
Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-2
Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .B-2
Training . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-3
iv PN 89-00002-00-150 Rev. A
xMAP Technology Contents
Luminex Sheath Delivery System C-1
Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1
Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1
Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-2
Sheath Delivery System Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . C-3
Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-3
Using the Sheath Delivery System . . . . . . . . . . . . . . . . . . . . . . . . C-5
Audible Alarm and Fault LED . . . . . . . . . . . . . . . . . . . . . . . . C-5
Maintenance and Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . C-6
Replacing the Sheath Delivery System with Sheath Bottles . C-6
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-6
Contact Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-7
Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-8
Index Index-1
PN 89-00002-00-150 Rev. A v
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
vi PN 89-00002-00-150 Rev. A
1
Introduction
The Luminex® 200™ system is a compact analysis unit consisting of
a Luminex analyzer, the Luminex XY Platform (Luminex
a PC, Luminex software, and reagents. The Luminex analyzer
integrates optics, fluidics, and advanced signal processing in a 17
inch by 20 inch footprint. The Luminex XYP provides efficient
handling of 96-well microtiter plates. The PC uses Luminex software
to control the analyzer. The optional Luminex Sheath Delivery
system (Luminex
from a 20 liter container.
Photodiodes and a photomultiplier tube receive fluorescent signals
from xMAP
and delivers the signals to a digital signal processor (DSP).
Proprietary algorithms function with the DSP to greatly increase the
sensitivity.
®
XYP™),
®
SD™) is used to provide sheath fluid directly
®
microspheres. The analyzer digitizes the waveforms
Hardware and preloaded software simplify installation and setup, and
with the help of this manual, you will be able to begin using xMAP
technology.
Principle of
Operation
PN 89-00002-00-150 Rev. A 1 - 1
The Luminex 200 system analyzes immunoassays, complex genetic
analyses, and enzymatic assays in one simple format. The reactants
(antibodies, oligonucleotides, substrates, etc.) of these assays are
anchored to the surfaces of uniquely fluorescent microscopic beads,
called microspheres. The unique fluorescent emission spectra of a
given microsphere identifies each of the assays performed
simultaneously on a single sample.
The Luminex analyzer individually analyz es xMA P mic r os ph ere s in
a flow stream. Every xMAP microsphere is accurately classified to
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
its own subset, based on its fluorescent signature. In addition, the
Luminex analyzer scans each microsphere for the presence of a
reporter fluorescence that quantifies the assay at the microsphere’s
surface.
Microtiter plates with 96 wells must be compatible with the Luminex
XYP plate holder. The following microtiter plate types are
compatible with the Luminex XYP instrument plate holder:
flatbottom, conical, round, filter bottom, or half-well plates of any
color. The overall plate height must be no more than 0.75” (19 mm).
Microtiter plates with 96 wells must be compatible with the Luminex
XYP instrument heater block temperature when performing heated
assays. The heater block temperature ranges from 35°C to 55°C
(95°F to 131°F) .
The system reads the wells in column-first order. An entire column
of wells is read; that is, well A1, B1, C1, D1, and so on to the end of
the column. Then the XY Platform moves over to the next column
and reads well A2, B2, C2, D2, and so on. When placing a plate on
the plate holder, place it so well A1 is in the upper-left corner of the
holder. You can run an entire plate, a consecutive range of samples
from the plate, or a single sample from the plate.
Basic Concepts Luminex has pioneered a versatile and robust technology for the
measurement of soluble analytes. The Luminex 200 system performs
simultaneous, discrete measurements of multiple microsphere-based
reactions from a single specimen aliquot. A clear understanding of
the concepts and functionality associated with the instrument and
xMAP microspheres contributes to greater success with this
technology. We present a brief overview of some of the basic
concepts. For more information, refer to Practical Flow Cytometry,
4th edition, by Howard M. Shapiro, M.D. (New York: Wiley-Liss
Inc., 2003).
Fluidics There are two fluidic paths in the Luminex analyzer. The first path is
a syringe-driven mechanism that controls the sample uptake. This
mechanism permits small sample uptake volumes from small
reaction volumes. The syringe-driven system transports a userspecified volume of sample from a microtiter plate to the cuvette.
The sample is injected into the cuvette at a steady rate for analysis.
After analysis, the sample path is purged with sheath fluid by the
second fluidics path. This process expels sheath fluid into the sample
1 - 2 PN 89-00002-00-150 Rev. A
xMAP Technology Introduction
container and effectively removes residual sample within the tubing,
valves, and probe. The second fluidics path is driven under positive
air pressure and supplies sheath fluid to the cuvette.
Excitation The excitation system in the Luminex analyzer uses two solid-state
lasers. A reporter laser excites fluorescent molecules bound to
biological reactants at the microsphere surface, and a classification
laser excites fluorochromes embedded in the microsphere. The lasers
illuminate the xMAP microspheres as they flow single-file through
the cuvette. These fluorescent signals are discriminated with
selective emission filters and are converted into intensity units by a
digital signal processor.
xMAP Microspheres The xMAP microspheres are highly uniform, polystyrene particles
that have been crosslinked during polymerization for great physical
and thermal stability. Varying amounts of fluorochromes embedded
within each microsphere give each microsphere set an unique
fluorescent signal. To ensure the stability of this address, it is
essential to protect the xMAP microspheres from light. Do not
subject xMAP microspheres to prolonged high temperatures, and
protect them from freeze/thaw manipulations.
Reporter Fluorochromes Each xMAP microsphere is dyed to emit light in a certain
classification channel. All xMAP microspheres of a given emission
represent a distinct assay within a multiplex of assays. A reporter
channel is used to detect fluorescence bound to the surface of each
microsphere, and each reporter emission quantitates each of the
distinct assays. Only one reporter emission is used for multi-analyte
assays in the Luminex analyzer. The Luminex analyzer uses an
excitation wavelength of 532 nm, and has an emission wavelength of
575 nm ± 12 nm.
Fluorochromes R-Phycoerythrin and Alexa
Luminex analyzer, but you are not limited to these two. You may
have success using other fluorochromes suc h as Cyanine 3.
Phycoerythrin
Formula weight (Daltons) 240,000 470
Absorbance max (nm) 480, 546, 565 531
Extinction max (M
Emission max (nm) 578 554
-1cm-1
1,960,000 83,800
)
®
532 work well with the
R-
Alexa
532
PN 89-00002-00-150 Rev. A 1 - 3
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
Quantum yield 0.82 0.8
Fluorescence
Compensation
Repetitive Microsphere
Measurements
Fluorescent spillover occurs when an emission spectrum overlaps
other emission criteria; therefore, selective filtering cannot occur. In
most analyzers, emission spillover is corrected using a technique
called compensation. Compensation involves subtraction of emission
percentage from another emission signal. In the Luminex 200
system, compensation is not necessary—the reporter signal does not
spill over into classification emissions.
Bound to reagents at the surfaces of the xMAP microspheres, the
reporter provides raw analytical data. Because a microsphere
suspension provides near liquid phase reaction kinetics, each
microsphere theoretically binds an equal number of reporter
molecules. Equal binding results in a statistically even distribution of
reporter on each microsphere in a set. When the Luminex analyzer
registers numerous events for each set, the system reports individual
population values based on an even coating of reporter. This means
that numerous replicates for each microsphere population are
measured from a single reaction vessel. The confidence in a given
measurement strengthens with increased replicate measurements. For
adequate confidence, 100 collected events per microsphere set in
each reaction vessel is usually sufficient. Measurements are reported
as the median for the xMAP microspheres having a specific color
signal.
About this Manual This manual introduces you to the Luminex 200 system. With step-
by-step instructions, the manual guides you through initial setup and
powering-on procedures. Next, it offers a concepts section which
describes some of the key topics encountered during system
operation. Once you have a working knowledge of the analyzer, the
manual’s text and figures lead you through the data collection
process, giving detailed explanations of available options and
offering examples when necessary. Glossary and troubleshooting
sections assist as references. In short, this manual instructs you
through each operational stage—from unpacking the Luminex
analyzer and XYP to collecting your data.
The conventions in this document assume a basic familiarity with
computers and a knowledge of Windows
document only one method of accessing a command, although many
commands are available through more than one method, such as
1 - 4 PN 89-00002-00-150 Rev. A
®
software. We typically
xMAP Technology Introduction
from the main menu bar, from the toolbar, and from menus that
appear when you right-click an area of the screen.
Technical
Assistance
You can find answers to frequently asked questions (FAQs) on our
website: http://www .luminexcorp.com. From the Support menu at the
top of the page, select FAQs.
For assistance, contact Luminex Technical Support via email. Users
may reach us at support@luminexcorp.com or
supporteurope@luminexcorp.com.
Users in the U.S. and Canada can call us at 1-877-785-BEAD (1877-785-2323) between the hours of 7:00 a.m. and 7:00 p.m. Central
Time, Monday through Friday. Users outside of the U.S. and Canada
can call us at +1 512-381-4397 between the hours of 7:00 a.m. and
7:00 p.m. Central Time, Monday through Friday.
Users in Europe by can call us at +31-162408333 between the hours
of 8:30 and 5:30, Central European Time, Monday through Friday.
PN 89-00002-00-150 Rev. A 1 - 5
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
1 - 6 PN 89-00002-00-150 Rev. A
Safety
Your safety is important. Please do not perform procedures on your
Luminex 200 system that are not specifically contained in this
manual, unless you are directed to do so by Luminex Technical
Support. This chapter discusses the following safety topics
• Intended Use
• Safety and Regulatory Symbols
• Warnings and Notes
• Safety Precautions
• Equipment Disposal
Intended Use The Luminex analyzer is design ed for a wide range of indoor
laboratory testing applications measuring biomolecular reactions on
the surfaces of xMAP microspheres. The Luminex 200 for LDS
Version 1.7 system is intended for general laboratory use.
The Luminex XYP instrument is intended for use with the Luminex
analyzer. With the Luminex XYP, you can process 96-well microtiter
plates without handling individual samples. The Luminex XYP
works with microtiter plates that are no thicker than 0.75 in.
PN 89-00002-00-150 Rev. A 2 - 1
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
Safety and
Regulatory Symbols
Symbol Description Symbol Description Symbol Description
Alternating current
(ac)
Protective ground
On
Off
WEEE - Do not throw
in trash
Temperature
Limitation
These symbols describe warnings, cautions, and general information
used in the operation of this analyzer.
Warning
(refer to manual)
Warning
(refer to manual)
Warning
(refer to manual)
Catalog Number Consult instructions
Batch Code Serial Number
Expiration Date Date of Manufacture
Warning
(refer to manual)
Warning
(refer to manual)
Warning (refer to
manual)
for use
Manufacturer
European Union
Conformity
Underwriter’s
Laboratory
US
Canadian
Underwriter’s
Laboratory
Warnings and Notes Informational notes and warnings may appear in this manual.
Note: A note provides general helpful information. No safety or
performance issues are involved.
Caution: This message is used in cases where the hazard is minor or
only potential hazard is present. Failure to comply with the caution
may result in potentially hazardous conditions.
Warning: This message is used in cases where danger to the
operator or to the performance of the instrument is present. Failure to
comply with the warning may result in incorrect performance,
instrument failure, invalid results, or hazard to the operator.
2 - 2 PN 89-00002-00-150 Rev. A
xMAP Technology Safety
Danger: This message is used in cases where significant risk of
serious injury or death is present.
Safety Precautions Please read the following safety information before setting up or
using the Luminex 200 system. A user should always be present
during system operation. This system contains electrical, mechanical,
and laser components which, if handled improperly, are potentially
harmful. In addition, biological hazards may be present during
Luminex analyzer operation. Therefore, Luminex Corporation
recommends that all Luminex analyzer users become familiar with
the specific safety advisories below, in addition to adhering to
standard laboratory safety practices. The protection provided by the
equipment may be impaired or the warranty voided if the equipment
is used in a manner not specified by Luminex Corporation.
Electrical The Luminex analyzer and Luminex XYP must be connected to
approved power sources.
Do not perform any maintenance or cleaning of the system’s
electrical components (except for the fuses).
This caution label appears on the back of Luminex analyzers and on
the Luminex XYP:
Figure 2-1. Fuse Replacement Warning Label
This label appears on the back of the Luminex analyzer and the
Luminex XYP.
PN 89-00002-00-150 Rev. A 2 - 3
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
Figure 2-2. European Safety Requirements Label
The Luminex analyzer complies with European Unio n (EU) saf e ty
requirements and, therefore, may be marketed in the Europe Single
Market.
The following voltage labels displays on the back of the Luminex
analyzer:
Figure 2-3. Luminex 200 Voltage Label (Coherent)
This voltage label displays on the back of the Luminex XYP:
Figure 2-4. Luminex XYP Voltage Label
2 - 4 PN 89-00002-00-150 Rev. A
xMAP Technology Safety
Fluidics The Luminex analyzer contains fluidics. In the event of a fluid leak,
turn off all power to the system and disconnect all power cords.
Warning: If the system is used
to test biological samples, use
your standard laboratory safety
practices when handling system
waste.
Remember that the on/off switch is not a disconnect means - the
power cord must be removed from the outlet. Contact Luminex
Corporation for further information.
You must manually monitor waste levels. Do not allow the waste
container to overflow. You can avoid this by emptying the waste
container each time you fill the sheath fluid container. Do not place
the waste container on top of the analyzer.
Be careful not to touch the seal in the waste bottle cap or allow it to
become wet or dirty. This could cause the bottle to become
pressurized, causing pressure errors in the system. If the seal gets
wet, let it air-dry. Touching the seal can contaminate it. If it gets
dirty, you should replace the waste bottle cap.
To maintain a stable flow rate, do not move the waste container or
waste line during system operation. While you can move the line
along a horizontal surface, do not permanently reroute the waste line
elevation without first contacting Technical Support. You can move
the line temporarily for cleaning and maintenance purposes.
United States and international regulations require the following
warnings to appear on the analyzer during operation and
maintenance.
This label appears on the back panel of the analyzer:
Figure 2-5. Laser Class Label
This label appears on the back panel of the Luminex analyzer:
PN 89-00002-00-150 Rev. A 2 - 5
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
Figure 2-6. Laser Caution Label
Do not remove the analyzer cover. When performing routine
maintenance, ensure that power to the analyzer is OFF and the power
cord is disconnected.
All laser apertures in the analyzer are contained within a protective
housing. This label appears on the optics cover within the Luminex
analyzer.
Figure 2-7. Laser Caution Label on Optics Cover
This label appears above the laser apertures located inside the optics
enclosure inside the Luminex analyzer.
2 - 6 PN 89-00002-00-150 Rev. A
xMAP Technology Safety
Figure 2-8. Avoid Exposure Label
Caution: Use of controls or adjustments or performance of
procedures other than those specified herein may result in
hazardous radiation exposure.
Caution: To avoid exposure to hazardous radiation, only perform
procedures and adjustments as specified in this manual.
Mechanical
Warning: During operation, this system contains exposed,
moving parts. Risk of personal injury is present. Observe all
warnings and cautions.
Note: Access doors must be
closed while operating the
instrument. An operator must
be present during operation of
the instrument.
Biological
Warning: During operation, this system contains exposed,
moving parts which could result in puncture hazard. Risk of
personal injury is present. Keep hands and fingers away from the
sample probe. Keep hands and fingers out of the Luminex XYP
slot during operation.
Warning: During operation, this system contains exposed,
moving parts which could result in pinch point hazard. Risk of
personal injury is present. Keep hands and fingers away from the
syringe arm.
Warning: Human/animal samples may contain biohazardous
infectious agents. Where exposure (including aerosol) to potentially
biohazardous materials exists, follow appropriate biosafety
procedures and use personal protective eq uipment, such as gloves,
gowns, laboratory coats, face shields or masks, eye pr ot ec tion , an d
ventilation devices. Observe all local, state, and federal biohazard
handling regulations when disposing of biohazardous waste
material.
PN 89-00002-00-150 Rev. A 2 - 7
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
Heat
Warning: The heater plate of the Luminex XYP may be hot and
could cause personal injury if touched. Do not touch the heater
plate.
Blue Indicator Light
The blue light above the sample arm simply indicates the on/off
status of the instrument, and is harmless. The blue light emitting
diode (LED) does not emit light in the UV spectrum.
Equipment Disposal The Waste Electrical and Electronic Equipment Directive 2002/96/
EC requires that you properly dispose of electrical and electronic
equipment when it reaches its end of life.
NOTE: This directive applies to customers in the European Union
(EU) only. Users outside of the EU may disregard this section.
TM
If you are disposing of a Luminex 200 Analyzer, SD
TM
Instrument, you must decontaminate the system. Refer to
XYP
“Decontaminating the Luminex Analyzer for Return Shipment” on
page 7-11. Next, call Luminex Technical Support for a Recycled
Returned Materials Authorization (RMA) number at +1 512-3814397 between the hours of 7:00 a.m. to 7:00 p.m. Central Time,
Monday through Friday. You will then return the equipment to:
Luminex Corporation
12201 Technology Blvd., Suite 130
Austin, TX 78727
System, or
For information on disposing of the PC or monitor, refer to
manufacturer documentation.
2 - 8 PN 89-00002-00-150 Rev. A
System Overview
3
Hardware The Luminex 200 for LDS Version 1.7 system includes the following
hardware:
• Luminex analyzer
• Computer (PC), monitor, and access ories
• Luminex XYP instrument (with long sample probe)
• Power cables
• Short sample probe
• Reservoir
•Shield
•Heater block
• Sheath fluid container
• Waste container
• Sheath fluid line
• Air line
• Sheath fluid intake line
• Serial cable
• USB cable
• CANBUS cable
• Sample probe height alignment kit
In addition, you can purchase the optional Luminex Sheath Delivery
system (Luminex SD).
xMAP Reagents • xMAP classification calibration microspheres (CAL1)
• xMAP reporter calibration microspheres (CAL2)
• Luminex xMAP Sheath Fluid
PN 89-00002-00-150 Rev. A 3 - 1
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
Required
Laboratory
Reagents
The following items are necessary to run the Luminex 200 system.
They are not provided by Luminex.
• Household bleach
• 70% isopropanol or 70% ethanol
• Mild detergent
• Distilled water
Software Luminex Data Collector software provides complete control of the
Luminex analyzer and performs real-time digital analysis of assays.
The Luminex 200 system is preloaded with the Luminex software.
However, we supply a software CD in case you need to reinstall the
software.
This software requires a dedicated system. Unauthorized additional
software is prohibited and may result in improper operation of the
system.
Luminex 200
Performance
Specifications
Speed • USB communications link for fast data transfer
• Automatic transfer of assay templates and new reagent
information into the system via a large capacity read/write CD
• Installation: < 4 hours
• System calibration: < 10 minutes
• Analyze one 96-well plate/hour depending on manufacturer’s kit
• Up to 100 xMAP microsphere sets per sample
• System warmup: 30 minutes. Systems that remain inactive for at
least four hours will require a warm-up to restart the lasers. After
acquiring sample, running system calibrators, running system
controls, and warming up the instrument, the system resets the
four-hour internal clock.
Accuracy and Precision • Sample uptake volume: ± 5%
• Classification of xMAP microspheres: > 80%
• Misclassification of xMAP microspheres: ≤ 2% - may vary by
xMAP microsphere product lines. Refer to the specific product
information sheet for further details.
• Internal sample carry over: < 0.9%
• Soluble background fluorescence emission at 575 nm
automatically subtracted from fluorescence intensity values
3 - 2 PN 89-00002-00-150 Rev. A
xMAP Technology System Overview
Sensitivity • Detect 1000 fluorochromes phycoerythrin (PE) per xMAP
microsphere
• Reporter channel dynamic range: 3.5 decades of detection
Capacity The specifications below reflect minimum capacity values:
• Distinguish a min imum of 1 to a maximum of 100 unique xMAP
microsphere sets in a single sample
• Detect and distinguish surface reporter fluorescence emissions at
575 nm on the surface of 1-100 unique xMAP microspheres sets
in a single sample
• Sample core: 15-20 µm core at 1 µL/sec. sample inject rate
• Maintain samples at a constant temperature from 35°C to 55°C
(95°F to 131°F)
• Automatic sampling from a 96-well plate
• Start sampling from any well position
• Sheath container and waste container hold enough volume to run
up to two 96-well plates between refills
• Microtiter plates with 96 wells must be compatible with the
Luminex XYP instrument plate holder. The following microtiter
plate types are compatible with the Luminex XYP instrument
plate holder: any color of flatbottom, conical, round, filter
bottom, or half-well plates. The overall plate must not have an
overall height of more than 0.75” (19 mm).
• Microtiter plates with 96 wells must be compatible with
Luminex XYP instrument heater block temperature from 35°C to
55°C (95°F to 131°F) when performing heated assays and using
the heater block.
Luminex 200
Analyzer
Specifications
PN 89-00002-00-150 Rev. A 3 - 3
• Indoor use only
• Operating temperature: 15°C to 30°C (59°F to 86°F)
• Humidity: 20% to 80%, noncondensing
• Altitude: Operation up to 2400 m (7874 ft.) above mean sea level
• Physical dimensions: 43 cm (17 inches) W x 50.5 cm (20 inches)
D x 24.5 cm (9.5 inches) H
• Weight: maximum of 25 kg (60 lbs.)
• UL installation category: UL Installation Category II, as defined
in Annex J of UL 61010A-1
• Pollution degree: UL Pollution Degree 2, as defined in Section
3.7.3.2 of UL 61010A-1
• Shipping and storage: The allowable shipping and storage
temperature and humidity ranges are 0°C to + 50°C and 20-80%
noncondensing, respectively
• Input voltage ran ge: 1 00 - 120 V~ ± 10%, 1.4 Amp, and 200-240
V~ ± 10%, 0.8 Amp, 47-63 Hz.
Luminex 200 User Manual for LDS Version 1.7 xMAP Technology
• AC inlet fuse: 3 Amp, 250 V~, fast acting
Optics • Reporter laser: 532 nm, nominal output 10-15 mW, maximum
500 mW, frequency-doubled diode; mode of operation,
continuous wave (CW)
• Classification laser: 635 nm, 9.1 mW ± 6%, maximum output 25
mW, diode; mode of operation, continuous wave (CW)
• Reporter detector: Photomultiplier tube, detection bandwidth of
565-585 nm
• Classification detector: Avalanche photo diodes with temperature
compensation
• Doublet discrimination detector: Avalanche photo diodes with
temperature compensation
Fluidics • Sheath flow rate 90 µL ± 5 µL/second
• Cuvette: 200 micron square flow channel
• Sample injection rate: 1 µL/second ± .05 µL/second
• Sample uptake volume: 20-200 µL
Electronics • Reporter channel detection: A/D resolution 14 bits
• Communications interface: USB
Luminex XYP
Specifications
• Ambient temperature: 15°C to 30°C (59°F to 86°F)
• Humidity: 20% to 80%, noncondensing
• Altitude: designed to operate at 2400 m (7874 ft.) above mean
sea level or below.
• Physical dimensions: 44 cm (17.25 in) W x 61 cm (24 in) D x 8
cm (3 in) H
• Weight: 15 kg (33 lbs)
• UL installation category: UL Installation Category II, as defined
in Annex J of UL 3101-1.
• Pollution degree: UL Pollution Degree 2, as defined in section
3.7.3.2 of UL 6101A-1
• Heater operating range: 35°C to 60°C (95°F to 140°F) with
tolerance 0°C to +2°C
• Input voltage range: 100-240 V~ ± 10%, 0.4 Amps, 47-63 Hz
• AC inlet fuse: 2 Amp, 250 V~, time lag
• Communications interface: RS 232
• Shipping and storage: allowable shipping and storage
temperature and humidity ranges, 0°C to +50°C and 20%-80%
noncondensing, respectively
3 - 4 PN 89-00002-00-150 Rev. A
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