Luminex 100E User Manual
Luminex 100E
™
User Manual
© LUMINEX® CORPORATION, 2004. All rights reserved. No part of this publication may be r eproduced,
transmitted, transcribed, or translated into any language or computer language, in any form or by any means
without prior express, written consent of:
LUMINEX CORPORATION
12212 Technology Boulevard
Austin, Texas 78727-6115
U.S.A.
V oice: (512) 219-8020
Fax: (512) 219-5195
Luminex 100ETM User Manual
CN-M028-01
PN 89-00002-00-013 Rev. D
July 2004
Luminex Corporation (Luminex) reserves the right to modify its product s and services at any time. This guide
is subject to change without notice. Although prepared to ensure accuracy, Luminex assumes no liability for
errors or omissions, or for any damages resulting from the application or use of this information.
The following are trademarks of Luminex: Luminex, Luminex HTS, Luminex 100, Luminex 100 IS, LabMAP,
xMAP, LumAvidin, Luminex SD, Luminex XYP, Luminex FlexMAP. All other trademarks, including Windows,
Cheminert, Pentium, and Dell
The contents of this manual and the associated Luminex software are the proper ty of L uminex and are
copyrighted. Except as specified in the End User License Agreement, any reproduction in whole or in part is
strictly prohibited.
are trademarks of their respective companies.
Contents
Introduction 1-1
Safety 2-1
Instrument Components 3-1
About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Customer Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Laser, Luminex 100E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Mechanical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Biological . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Decontamination of the Luminex 100E for Return Shipment . . . .2-6
Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
xMAP Reagents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Laboratory Reagents Required . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 -2
Other Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Luminex Real-time (LXR) Library. . . . . . . . . . . . . . . . . . . . . . . . .3-3
Sensitivity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
General, Luminex 100E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5
Electronics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5
System Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5
User-Accessible Components. . . . . . . . . . . . . . . . . . . . . . . . . .3-5
Power Input Connector. . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5
P1 Communications Port (DB9-PIN). . . . . . . . . . . . . . . . .3-7
P2 Synchronization Port (DB15-PIN) . . . . . . . . . . . . . . . .3-8
Air Filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-10
Fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-10
Regulated Flow Sheath Supply . . . . . . . . . . . . . . . . . . . .3-10
Sample Input Tube. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-11
Optical. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-11
Installing the Luminex 100E into the Host System. . . . . . . . . . . .3-12
PN 89-00002-00-013 Rev. D i
Luminex® 100E™ User Manual Version 1.0 xMAP Technology
Maintenance and Cleaning 4-1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Daily. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Before Running Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
After Running Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Monthly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Instrument Calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Calibrate the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Semi-Annually . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Remove or Replace the Syringe Seal. . . . . . . . . . . . . . . . . . . . 4-3
As Required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Instrument Front Panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Remove the Instrument Front Panel . . . . . . . . . . . . . . . . . 4-4
Reinstall the Instrument Front Panel. . . . . . . . . . . . . . . . . 4-4
Air Filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Remove the Air Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Clean the Air Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Reinstall the Air Filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Syringe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Remove the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Replace the Syringe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Verify Syringe Operation . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Product Numbers 5-1
Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
xMAP Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Index Index-1
ii PN 89-00002-00-013 Rev. D
Introduction
1
About This Manual This manual introduces you to the Luminex
explains the features of the Luminex 100E analyzer and provides
instructions on installation and use of the analyzer.
®
100E™ analyzer. It
Intended Use The Luminex 100E analyzer is designed as a component in a host
system. This component is designed for a wide range of laboratory
testing applications measuring biomolecular reactions on the surfaces
of xMAP
indoor use only.
Systems analysts, software designers, and programmers who need an
overall perspective of the capabilities and functions of the Luminex
Runtime (LXR) should refer to the LXR Programmer’s Reference
provided by the LXR Software Development Kit (LXR SDK)
version 2.0 or greater for more information. The Luminex 100E
analyzer responds to applications developed using the LXR SDK
version 2.0. LXR SDK version 2.0 supplies the runtime software and
several utility applications needed to develop new applications and
maintain the Luminex 100E analyzer.
®
and customer-specific microspheres. It is intended for
LXR is packaged as a collection of Windows
The interfaces of the various components are written as Component
Object Model (COM) objects that conform to the Microsoft
automation standards. This COM Automation support allows access
to the Luminex 100E analyzer via programming and scripting
software.
PN 89-00002-00-013 Rev. D 1 - 1
®
binary and data files.
®
Luminex 100E User Manual xMAP Technology
Technical Support You can contact Luminex Technical Support for questions or
concerns regarding the Luminex 100E analyzer.
Users in the U.S. and Canada may call 1-877-785-BEAD (-2323)
between the hours of 7:00 a.m. and 7:00 p.m. Central Time, Monday
through Friday for assistance. Users outside of the U.S. and Canada
can contact us at + 1 512-381-4397 between the hours of 7:00 a.m. to
7:00 p.m. Central Time, Monday through Friday.
Inquiries may also be sent by email to support@luminexcorp.com.
1 - 2 PN 89-00002-00-013 Rev. D
Safety
2
Symbols These symbols describe warnings, cautions, and general information
used in the operation of this Luminex 100E analyzer. These symbols
are further defined under “Safety Precautions.”
Number Symbol Description
1 Protective ground
2 Warning (refer to manual)
3 Warning (refer to manual)
4 Warning (refer to manual)
Safety Precautions Read the following safety information before setting up or using the
Luminex 100E analyzer. The user should be present during
operation. The Luminex 100E analyzer contains electrical,
mechanical, and laser components which, if handled improperly, are
potentially harmful. In addition, biological hazards may be present
during system operation. Therefore, we recommend that all Luminex
100E analyzer users become familiar with the specific safety
advisories below, in addition to adhering to standard laboratory
safety practices. The protection provided by the equipment may be
impaired or the warranty voided if the system is used in a manner not
specified by the instructions or Luminex Corporation.
PN 89-00002-00-013 Rev. D 2 - 1
Luminex 100E User Manual xMAP Technology
The following labels appear on the back panel of the Luminex 100E
analyzer.
Figure 1. Voltage Label
Figure 2. FCC Label
Note: This equipment has been tested and found to comply with the
limits for a Class B digital device, pursuant to part 15 of the FCC
Rules. These limits are designed to provide reasonable protection
against harmful interference in a residential installation. This
equipment generates, uses and can radiate radio frequency energy
and, if not installed and used in accordance with the instructions,
may cause harmful interference to radio communications. However,
there is no guarantee that interference will not occur in a particular
installation. If this equipment does cause harmful interference to
radio or television reception, which can be determined by turning the
equipment off and on, the user is encouraged to try to correct the
interference by one or more of the following measures:
• Reorient or relocate the receiving antenna.
• Increase the separation between the equipment and the
receiver.
• Connect the equipment into an outlet on a circuit different
from that to which the receiver is connected.
• Consult the dealer or an experienced radio/TV technician for
help.
2 - 2 PN 89-00002-00-013 Rev. D
xMAP Technology Safety
Do not perform any maintenance or cleaning of the electrical
.
components of this system.
The Luminex 100E analyzer complies with European Union (EU)
safety requirements.
This analyzer contains fluidic components. In the event of a fluidic
leak, disconnect the power. Contact Luminex Corporation for further
information.
Laser, Luminex 100E United States and international regulations require the following
warnings to appear on the analyzer during operation and
maintenance. The labels in this section are representative.
Figure 3. Laser Class Label
The following label appears on the back panel of the Luminex 100E
analyzer.
Figure 4. Laser Caution Label
Under NO circumstances should you remove the Luminex 100E
analyzer cover or this label! When performing routine
maintenance, disconnect power to the analyzer.
PN 89-00002-00-013 Rev. D 2 - 3
Luminex 100E User Manual xMAP Technology
All Luminex 100E analyzer laser apertures are contained inside a
protective housing within the analyzer. This label appears on the
optics cover within the Luminex 100E analyzer.
Mechanical
Figure 5. Laser Caution Label on Optics Cover
CAUTION — Use of controls or adjustments or performance of
procedures other than those specified herein may result in hazardous
radiation exposure.
Attention — L’utilisation des commandes ou réglages ou l’exéction
des procédures autres que celles spécifiées dans les présentes
prescriptions peuvent être cause d’une exposition à un rayonnement
dangereux.
This label appears above the laser apertures located inside the optics
enclosure inside the Luminex 100E analyzer.
Figure 6. Avoid Exposure Label
Warning: During operation, this analyzer contains moving parts.
Risk of personal injury is present. Observe all warnings and cautions.
2 - 4 PN 89-00002-00-013 Rev. D
xMAP Technology Safety
Warning: During operation, this analyzer contains moving parts that
result in a pinch point hazard. Risk of personal injury is present.
Keep hands and fingers away from the syringe arm.
Biological Warning: All human/animal samples may contain hazardous
infection agents.Where exposure (including aerosol) to potentially
biohazardous materials exists, operators should follow appropriate
biosafety procedures and use personal protective equipment, such as
gloves, gowns, laboratory coats, face shields (or mask and eye
protections), and ventilation devices.
Observe all local, state, and federal biohazard handling regulations
when disposing of biohazardous waste material.
PN 89-00002-00-013 Rev. D 2 - 5
Luminex 100E User Manual xMAP Technology
Decontaminating
the Luminex 100E
Analyzer for Return
Shipment
Note: You are responsible for
decontaminating the unit prior
to shipment.
Luminex Technical Support will provide a Return Material
Authorization (RMA) number if they direct you to return the
analyzer to Luminex; they will also instruct you in returning the
analyzer according to Luminex procedures.
Before returning the Luminex 100E analyzer, sanitize and
decontaminate the accessible surfaces and internal fluidics system.
This is particularly important if you have analyzed biohazardous
samples. Make a copy of this page to complete and return with the
analyzer. Submit the completed checklist, both signed and dated,
with the Luminex 100E analyzer.
1. Replace the fluid in the sheath system with a freshly prepared
solution of 10% - 20% household bleach and water.
2. Using a solution of 10% - 20% household bleach and water,
sanitize the analyzer using maintenance tools in the LXR library.
Follow this by washing twice with distilled water.
3. Disconnect the power plug from the analyzer
4. Disconnect the fluidic lines.
5. Disconnect the sample probe from the host’s sample mount
sample probe line.
6. Wash all exterior surfaces with a mild detergent, followed by a
10%-20% bleach solution.
7. Remove the air filter from the analyzer and clean all accessible
surfaces with detergent followed by a 10%-20% bleach solution.
8. Pack the analyzer using a biohazard bag, place it in an approved
shipment box, and then insert it into a shipping crate. Attach this
checklist to the top of the corrugated box prior to packaging in
the crate.
Was there an internal leak in the system? Yes No
Print Name:___________________________ ____ _____ ____
Signature:__________________________________________
Date:______________________________________________
2 - 6 PN 89-00002-00-013 Rev. D
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