Life technologies Invitrogen Tali User Manual

user guide

Tali™ Image-Based Cytometer

Catalog Number T10796

Publication Part Number MAN0003766

Revision Date 28 April 2011

Contents

Product Contents....................................................................................................................................3

Introduction ..................................................................................................................4

Description of Tali™ Image-Based Cytometer........................................................................................4

Specifications..........................................................................................................................................9

Methods ......................................................................................................................11

Getting Started......................................................................................................................................11

Guidelines for Performing Tali™ Assays...............................................................................................14

Select a Tali™ Assay ..............................................................................................................................15

Load Sample ......................................................................................................................................... 16

Focus Image..........................................................................................................................................18

Measure the Background (optional).....................................................................................................19

Run Sample...........................................................................................................................................20

Analyze Data .........................................................................................................................................25

Export Data ...........................................................................................................................................28

Maintenance ...............................................................................................................31

Update the Tali™ Image-Based Cytometer Firmware..........................................................................31

Adjust the Camera Alignment ..............................................................................................................32

Calibrate the Tali™ Image-Based Cytometer .......................................................................................34

Clean the Tali™ Image-Based Cytometer.............................................................................................36

Appendix..................................................................................................................... 37

Safety Information ................................................................................................................................37

Accessory Products ..............................................................................................................................39

Technical Support................................................................................................................................. 40

Purchaser Notification..........................................................................................................................41

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