Life technologies EVOS FL, EVOS FL Color Quick Reference

5

EVOS® FL
EVOS

®

FLColor

For Research Use Only. Not for use in diagnostic procedures.

QUICK REFERENCE

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18 March 2013

Focusing
knobs

Power
switch

Objective
selection
wheel

Condenser
slider slot

Power
input jack

Phase
annulus
selector

Stage
X-axis
knob

USB and
DVI ports

Coarse stage
positioning
knobs

X-axis
stage
brake

Stage
Y-axis
knob

Y-axis
stage
brake

Light cube
selection
lever

Vessel
holder

LCD
screen

Imaging Systems

for Fluorescence and TransmittedLight
Applications

Catalog Numbers AME4300, AMEFC4300

Doc. Part no. ZP-PKGA-0494
Pub. no. MAN0007717
Rev. 2.0

6. Turn on illumination with the LIGHT ON

button located on the left side of the

control bar.

7. Focus the sample with focusing knobs



.

8. Optional: To take a picture of the transmitted

light image, click the Capture button on
the control bar.

9. Place the light shield box



on the stage,

over the sample.

10. Move the light cube selection lever



to the
desired fluorescence channel (the Channel
Bar will highlight the selected light cube).

11. With the Find & Focus tab active, turn on

fluorescence illumination using the LIGHT
ON button.

12. Adjust the focus as necessary.

13. Adjust the Illumination Intensity slider on

the control bar as needed.

14. Click the Capture button.

15. Repeat steps 10–14 to acquire each

fluorescence channel.

16. Click the Overlay tab to show all channels

in color overlay mode.

17. Adjust Brightness and Contrast for each

channel to bring them into desired balance.

18. Click the Save button to save the color

image (refer to the EVOS

®

FL Imaging

SystemUser Guide).

Overview

The EVOS® FL and EVOS® FL Color Imaging
Systems have two types of controls: manual

and onscreen. Manual controls include the the
stage X–Y axis knobs, focusing knobs, objective
selection wheel, phase annuli selector, and the
light cube selection lever. Onscreen controls are
located in the control bar at the bottom of the
display screen. The channel bar at the top of the
display screen shows the selected light cube or
transmitted light position.

2

Helpful Tips

 In Find & Focus or Actual Mode, the Color option

(for EVOS

®

FL, Cat. no. AME4300) can be turned
off to display a grayscale image. This often shows
more details than a color image.

3

Control bar

Channel bar

Basic Operation

Note: For detailed instructions, refer to the EVOS®
FL Imaging SystemUser Guide, which is provided
on the USB flash drive. You can also dowload it from
the EVOS

®

FLImaging System product page at

www.lifetechnologies.com.

1. Turn on the instrument using the power

switch



on the right side of the base.

2. Plug a USB flash drive into one of the USB

ports



on the right side of the instrument.

3. Place the sample



on the stage, using a

vessel holder if needed.

4. Set magnification with the objective selection

wheel



on the front of the instrument.

5. Pull the light cube selection lever



(left
side of base) all the way toward the front of
the instrument (the Channel Bar will
highlight the “Transmitted” position).

CAUTION! UV HAZARD. Avoid exposure
to beam and use protective shields.
NEVER look directly at UV light!

4

Onscreen Controls

1. Active Channel (highlighted)

2. Login Button

3. Control Bar Tabs

• Find & Focus

• Actual

- Exposure Time Slider

• Overlay

- Overlay Color Dialogue Box

4. Light ON/OFF Button

5. Illumination Slider

6. Exposure Time Slider

7. Image Capture Button

8. Color Option

9. Scalebar/Toolbar Options

10. Setting Control Button

11. Save Image Button

12. Info Display Bar

13. Selected Objective

For EVOS

®

FL Color (Cat. no. AMEFC4300), use the Color Adjustment button to fine tune your live

image Brightness, Contrast, Saturation, and Hue prior to capture.

 In Find & Focus Mode, the exposure time is set to 100 ms to assist real-time focusing, moving the

stage, etc. The illumination level is approximately 60% of the amount used for image capture, in
order to minimize photobleaching and phototoxicity. Clicking Capture results in brighter illumination
and a longer exposure time during image capture to provide a high-quality image.

 In Actual Mode, turning on the illumination results in full-powered illumination and actual exposure

times for live viewing of the sample. With longer exposure times (more than 200 ms) there will be a
lag between moving the focus knob and seeing the focus change onscreen.

1

4

9

10 116512

13

8

7

3

2

1

2

3

5

6

LIGHT ON button Illumination slider Capture button

7

5

Overlay tab Brightness and Contrast

Save button

Color option Color Adjustment button

4