Levenhuk 740T User Manual

Levenhuk 700 Series

Biological Microscopes

Levenhuk 700M

Levenhuk 720B Levenhuk 740T

Levenhuk D740T

User Manual Инструкция по эксплуатации

Приближает с удовольствием

1 Base 2 Arm 3 Focus adjustment knob 4 Eyepiece head 5 Eyepieces 6 Revolving nosepiece 7 Objectives 8 Stage 9 Abbe condenser

10 Illumination 11 Brightness adjustment

Caution: Please remember that mains voltage in most European countries is 220–240V. If you want to use your device in a country with a different mains voltage standard, remember that use of a converter is absolutely necessary.

Внимание! Помните, что напряжение сети в России и большинстве европейских стран составляет 220–240 В. Если вы хотите использовать устройство в стране с другим стандартом сетевого напряжения, необходимо включать его в розетку только через соответствующий конвертер (преобразователь напряжения).

1 Основание 2 Опорная стойка 3 Ручка регулировки фокуса 4 Окулярная насадка 5 Окуляры 6 Револьверная головка 7 Объективы 8 Предметный столик 9 Конденсор Аббе

10 Подсветка 11 Регулировка яркости

Caution: Children should use the microscope under an adult’s supervision only.

Внимание: дети должны пользоваться микроскопом только под

присмотром взрослых.

Levenhuk 700 Series Biological Microscopes

General Information

Levenhuk 700 Series biological microscopes are modern state-of-the-art optical instruments made with great attention to detail. They are a perfect choice for specialists in all scientific areas. Levenhuk 700 Series microscopes are mainly used for observing and testing biological samples. They perform well during clinical research and tests; teaching demonstrations; in medical and health establishments, laboratories, universities and schools; and may be used for scientific research in agriculture and microbiology.

Package

The kit includes: microscope body eyepiece head: monocular (700M), binocular (720B) or trinocular (740T, D740T) achromatic objectives: 4x, 10x, 40xs, 100xs (oil) eyepieces: WF10x and H20x blue filter vial of immersion oil dust cover user manual and warranty

The Levenhuk D740T kit additionally includes: 5Mpx digital camera, a USB-cable and a CD with Levenhuk ToupView software & drivers.

Microscope parts

Base. It supports the weight of the microscope, and houses the illumination source, electronics

and control mechanisms. Arm. This piece holds the base, the stage and the head of the microscope together. Coarse and fine focus systems provide for smooth vertical movements of the stage. Rack-and-pinion mechanism. The stage with the condenser are mounted on the arm and are moving vertically along it. For additional precision, a condenser may be adjusted separately. Head. A monocular (Levenhuk 700M), binocular (Levenhuk 720B) or a trinocular (Levenhuk 740T and D740T) head is mounted at a 45° angle at the upper end of the arm and is 360° rotatable. Eyepieces and objectives. Consist of lenses that allow magnifying the image. High-quality achromatic objectives with 4x, 10x, 40x and 100x magnifications provide for sharp and bright images. The total magnification is calculated by multiplying the eyepiece magnification to the objective magnification. Thus, the available magnifications are 40x, 100x, 400x and 1000x with the wide field WF10x objective, and 80x, 200x, 800x and 2000x with the H20x objective. Revolving nosepiece. The quadruple revolving nosepiece allows you to change objectives smoothly and easily. Stage. Sturdy and reliable stage with a specimen holder can be used to move your slides while observing them. The lower illumination light passes through the opening in the middle of the stage. Condenser. Abbe condenser, 1.25 N.A. iris diaphragm is a system of lenses located below the stage that allows gathering and directing the light rays. Focusing knob. A coarse and fine focusing system allows moving the stage up and down adjusting the image sharpness. Lower illumination. LED illumination with adjustable brightness allows observing transparent objects. The illumination system can be powered by A/C.

Operating principle and illumination

Image creation system: objective (6), prism (7) and eyepiece (9). The

objective lens (6) magnifies a specimen (0), light rays pass through the prism (7), refract at a 45° angle and create an image in the eyepiece. Total magnification may be calculated by multiplying magnifications of the eyepiece and the objective used.

Illumination system: lamp (1), collector lens (2), diaphragm (3) and condenser (4). Light emitted from the lamp (1) passes through the collector lens (2) and illuminates the diaphragm (3). Then, the light is focused by the condenser (4). This illumination system is used for observations of a specimen (0) in transmitted light. You can also use a different type of illumination (e.g. a reflector) for observations in reflected light.

Using the microscope

Getting started

Unpack the microscope and make sure all parts are available. Move the stage to the lowermost position using the focusing knob and turn on the illumination or adjust the reflector to evenly illuminate the specimen within the field of view. Connect the microscope to A/C power using the A/C adapter. Insert the eyepiece into the eyepiece tube. Slowly adjust the illumination brightness, from dark to light. When you finish your observations, always dim the illumination before unplugging the power cable. If you need to change the light bulb, unplug your microscope first.

Focusing

Place a specimen on the stage and fix it with the holders. Select the 4x objective rotating the revolving nosepiece. Center the observed specimen in the field of view. Rotate the focusing knob to slowly raise the stage until the objective is close to the specimen; keep checking the distance between the objective and the object to avoid their contact. CAUTION: The objective should not touch the specimen, otherwise the objective or/and the specimen might be damaged. Look through the installed eyepiece and lower the stage slowly rotating the focusing knob until you see the sharp image of the observed specimen. Such adjustment protects the frontal lens from contacting the object when you use objectives of other magnifications; though, slight refocusing might be required. If the image is too bright, adjust the diaphragm disk until the passing light ray is reduced to a comfortable brightness level. If the image is too dark, increase the light ray.

Selecting the objective

Start your observations with the lowest magnification objective (4x) and select a specimen segment for detailed research. Then move the specimen to center the selected segment in the field of view, to make sure it keeps centered when the objective is changed to a more powerful one. Once the segment is selected, you should center its image in the microscope's field of view as precisely as possible. Otherwise, the desired segment might fail to center in the field of view of the higher power objective. Now you can switch to a more powerful objective by rotating the revolving nosepiece. Adjust the image focus, the iris diaphragm and the illumination brightness if required. When using a 100x objective lens, you have to fill the space between the lens and the specimen with immersion oil. There should be no air bubbles in the oil, as they might reduce the resulting quality of the image. You must wipe the 100x objective clear after using it.

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