Leica FL800 User Manual

Leica FL800

Integrated Vascular Fluorescence

Living up to Life

Pioneering Fluorescence Innovation

The development of fluorescence microscopy has a long tradition

at Leica Microsystems, dating back to the beginning of the 20th

century. Today, the successful integration of Leica Microsystems’

high-resolution surgical optics with the science of fluorescence

provides brand new possibilities in vascular procedures. Thanks

to this progress even small arteries and veins can be visualized in

real time through the optics of a surgical microscope.

The Leica FL800 vascular fluorescence

device has received FDA 510(k)

clearance for heart, plastic, and

neuro surgery. For additional

information please contact your local

Leica Microsystems representative.

This ability to monitor blood flow in tissue and vessels intra-opera-

tively during surgery can benefit surgical outcomes.

The Leica FL800’s integration with the surgical microscope makes

vascular fluorescence a seamless part of surgery.

Visualize Blood Flow

ey e
sensitivity
spectrum

emission spectrum

0.2

0.4

0.6

0.8

1.0

400 500 600 700

800

/nm

excitation
spectrum

non visible

video filter

excitation
spectrum

The Leica FL800 is used in conjunction with the fluorescent agent

IndoCyanineGreen (ICG) to view vascular blood flow directly

through the surgical microscope eyepieces or on a video monitor.

Obtaining this ICG-fluorescence information at the near infrared

(NIR) is a fast, easy procedure. To change from white light to NIR

mode, the surgeon simply pushes a button found on the pistol grip

of the surgical microscope.

The ICG Process

ICG perfusion is detectable because it excites at 800nm light and

then emits fluorescence at 835nm. The 835nm light is filtered away

from the normal white light and is detected by a special NIR

CCD-camera. The CCD-camera converts the 835nm light (invisible

to the human eye) to white light and projects it to a standard video

monitor and/or recording device.

View: White light mode

Near infrared overlay in the

left eyepiece, the anatomical view in

the right eyepiece.

ICG Spectras