Leica FL400 User Manual

Leica FL400
Invisible Becomes Visible

Living up to Life

Pioneering Fluorescence Innovation

The development of fluorescence microscopy has a long tradition

at Leica Microsystems, dating back to the beginning of the 20th

century. An important contribution to biological research, fluores-

cence science now integrates with the technology of the surgical

microscope to provide state-of-the-art oncological fluorescence.

The Leica FL400 enables fluorescence guided tumor resection. In

addition to the visible white light image, the Leica FL400 provides

the surgeon with information from fluorescence imaging and

the infrared spectrum, previously not visible to the human eye.

The ability to view fluorescence images intraoperatively through

the microscope’s eyepieces during surgery can benefit surgical

* The Leica FL400 is not approved

for sale in the USA, Japan,

and some other countries.

Please check the status of approval

with your local Leica representative.

2

outcomes.

Oncological Fluorescence

*

Photo Dynamic Imaging (PDI) during surgery combines a tumor-

selective photosensitizer, excitation light of an appropriate wave-

length, and a well-adjusted observation spectrum. Leica Micro-

systems provides the ideal combination of filters and crisp, clear

optics to provide good orientation in the resection area. The vital

tumor shines with red sensitizer fluorescence in good contrast to

normal tissue under blue light illumination.

Observation modes

Changing observation modes is easy. To switch from white light

mode to fluorescence mode and back requires only the click of

a button on the handle or the foot pedal. The Leica CAN bus con-

trols the type of illumination, the observation filters for different

fluorescence applications, and an optional mode-controlled video

camera specifically aligned for fluorescence.

View: White light mode

5-ALA Spectras

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8.0

6.0

4.0

2.0

excitation spectrum

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3

IntenseBlue

Using two light sources (Leica M525) or a high

efficient 400W light source (Leica M720),

the Leica FL400 offers the best excitation and

bright fluorescence of the protoporpherin.

Furthermore, the system supports the surgeon

with longer resection cycles in the blue light

mode, without the eyestrain of frequent

changes to white light.

TM

Leica M720 FL400 illumination module

The PDI Application

Surgeons familiar with Photo Dynamic Imaging (PDI) comment

that it is very easy to use. The patient orally receives the 5-ALA

dissolved in water, thus placing little strain on the patient, and has

almost no side effects.

Tum or resection can be performed not only in white light mode but

also in blue light mode. Blue light mode requires sufficient bright-

ness in order to resect the residuals of the tumor, which always

appear red or pink under blue light.

The Leica FL400 used for PDI reveals many new possibilities – its

potential is not yet fully realized. Overall, it represents an out-

standing new, straightforward method of intraoperative tumor

resectioning.

Malignant glioma,

white light mode

Malignant glioma,

blue light mode

Pictures taken
with Leica FL400

5-ALA Operation

5

The Leica FL400 System

The intensified and homogeneous excitation light of the Leica M525 OH4 with the
Karl Storz D light C or the Leica M720 OH5 with 400W Xenon power offers a bright and
clear fluorescence view.
A patented long pass filter switched into the observation beampathes provides a
well-adjusted observation and a perfect photo dynamic signal.

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The Best Possible Image

The fluorescence technique

The Leica M720 OH5 and Leica M525 OH4 can be easily upgraded to use blue light

fluorescence. The illumination systems, the observation filters and an optional blue

mode-optimized video camera interact automatically with a simple push of a

button found on the pistol grip of the surgical microscope. This offers bright blue

illumination and an easy and ergonomic change of the observation modes.

The Leica M720 and Leica M525 surgical microscopes

The Leica M720 OH5 and Leica M525 OH4 with integrated Leica FL400 5-ALA

fluorescence module offer the easiest movement, best ergonomics and highest

quality optics of any surgical microscope on the market today. Leica’s premium

OptiChromeTMOptics provides outstanding contrast, sharpness, resolution and

color fidelity. The “light touch” maneuverability of the Leica floorstands OH5 and

OH4 give the surgeon the perfect blend of stability and ease-of-use for micro-

surgical procedure.

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10 M1 750 0en/C • © Leica Microsystems (Schweiz) AG • CH-9435 Heerbrugg, 2010 • Printed in Switzerland – I.2010 – RDV – Illustrations, descriptions and technical data are not binding and may be changed without notice.