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PHOSPHONATE TEST KIT
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DROP COUNT METHOD
MODEL OP-DC · CODE 7634-DC
QUANTITY CONTENTS CODE
15 mL *Hydrochloric Acid, 1.0N *6130-E
30 mL Meta Cresol Purple Indicator 2202-G
15 mL Sodium Thiosulfate, 0.1N 6155-E
10 g Xylenol Orange Powder 6165-D
60 mL Thorium Nitrate 6158PS-H
1 Test Tube, 10 mL, glass, w/cap 0822
1 Test Tube, 2.5-5-10-15-20 mL,
glass, w/cap
1 Spoon, 0.1 g, plastic 0699
1 Pipet, plain, glass, w/cap 0371
1 pH Comparator, Low-High 7712
*WARNING: Reagents marked with an * are considered to be potential
health hazards. To view or print a Material Safety Data Sheet (MSDS) for
these reagents go to www.lamotte.com. To obtain a printed copy, contact
LaMotte by e-mail, phone or fax.
To order individual reagents or test kit components, use the specified code
number.
0970-S
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USE OF THE OCTET COM PARA TOR
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The Octet Comparator contains eight permanent
color standards. A test sample is inserted into the
openings in the top of the comparator. The sample
can then be compared to four color standards at
once, and the value read off the comparator. For
optimum color comparison, the comparator should
be positioned between the operator and a light
source, so that the light enters through the special
light-diffusing screen in the back of the comparator.
Avoid viewing the comparator against direct
sunlight or an irregularly lighted background.
pH AD JUST MENT
Prior to performing the actual test titration, the pH of the test sample
must be adjusted to approximately pH 2.6. The pH adjustment is made
by adding *Hydrochloric Acid to the test sample. The amount of
*Hydrochloric Acid to be added is determined as follows:
A. Fill the test tube (0822) to the 10 mL line with sample water.
B. Add 14 drops of Meta Cresol Purple Indicator (2202). A color
somewhere between yellow and purple will develop, depending on
the pH of the sample.
C. Add *Hydrochloric Acid (6130) one drop at a time, mixing
between drops. Count the number of drops and continually
compare the color of the sample against the two pH color standards
in the pH Low-High Comparator (7712). When the sample color is
between these two standard colors, the sample has been adjusted to
the proper pH. The sample color must not be too yellow or too red.
D. Record the number of drops of *Hydrochloric Acid used in Step C.
The same number of drops will be added to the sample in Step 3 of
the Test Procedure. Discard this sample.
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PRO CE DURE
1. Fill the test tube (0970-S) to the 10 mL line with sample water.
2. Add 1 drop of Sodium Thiosulfate (6155).
3. Add the exact number of drops of *Hydrochloric Acid (6130)
recorded in Step D.
4. Use the 0.1 g measuring spoon (0699) to add one level measure of
Xylenol Orange Powder (6165). Swirl to dissolve. The solution will
turn yellow.
5. Use the pipet assembly (0371) to add Thorium Nitrate (6158PS)
one drop at a time, mixing after each drop, until the sample color
changes to pink. Be sure to hold the pipet in a vertical position to
form uniformly sized drops.
6. Record the number of drops required to obtain the endpoint color
change.
7. For most accurate results, a blank test should be run on a sample of
the water containing no Phosphonate. Any result from this blank
test should be subtracted from the result recorded in Step 6 above.
8. This test has been calibrated for Dequest 2006. When a different
compound is to be tested, the amount of Thorium Nitrate added
should be multiplied by a conversion factor to determine ppm
Phosphonate.
Phosphonate Compound Name Factor
Dequest 2000 AMP(NTP) 0.8
Dequest 2006 NaAMP 1.0
Dequest 2010 HEDP(A) 0.6
Belcor 575 0.5
NOTE: If any other Phosphonate compound is used, the factor
must be determined experimentally using standard solutions of that
compound.
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LAMOTTE COM PANY
Helping Peo ple Solve An a lyt i cal Chal lenges
SM
PO Box 329 · Chestertown · Mary land · 21620 · USA
800-344-3100 · 410-778-3100 (Out side U.S.A.) · Fax 410-778-6394
Visit us on the web www.lamotte.com
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