NITRATE/NITRITE KIT
OCTA-SLIDE 2, 0.25-10.0 ppm
CODE 3519-01
QUANTITY CONTENTS CODE
2 x 120 mL *Mixed Acid Reagent *V-6278-J
5 g *Nitrate Reducing Reagent *V-6279-C
5 g *Color Developing Reagent *V-6281-C
2 Spoons, 0.1g, plastic 0699
3 Test Tubes, 2.5-5-10 mL, w/caps, plastic 0106
1 Water Sample Bottle 0688
1 Dispenser Cap 0692
1 Nitrate-N Octa-Slide 2 Bar, 0.25-10.0 ppm 3109-01
1 Octa-Slide 2 Viewer 1101
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents go to
www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
To order individual reagents or test kit components, use the specifi ed code number.
NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap
for refi ll reagents.
USE OF THE OCTA-SLIDE 2 VIEWER
The Octa-Slide 2 Viewer should be held so
non-direct light enters through the back of
the Viewer. Insert the reacted sample into
the top of the Viewer. Slide the Octa-Slide 2
Bar into the Viewer and match the color of
the reaction to the color standards.
WARNING! This set contains chemicals
that may be harmful if misued. Read
cautions on individual containers
carefully. Not to be used by children
except under adult supervision.
Nitrites can cause serious interference in the Nitrate test. To obtain the actual nitratenitrogen concentration, follow Procedures A & B. Subtract the nitrite reading obtained
in Procedure B (as ppm nitrate-nitrogen) from the nitrate reading obtained in
Procedure A (as nitrate-nitrogen).
The best results are obtained when the sample and reagents are at 23 ±2°C.
PROCEDURE A - NITRATE TEST
1. Fill sample bottle (0688) with sample water.
2. Fill test tube (0106) to the 2.5 mL line with water from the sample bottle.
3. Dilute to the 5.0 mL line with *Mixed Acid Reagent (V-6278). Cap and mix.
Wait two minutes.
4. Use the 0.1 g spoon (0699) to add one level measure (avoid any excess) of
*Nitrate Reducing Reagent (V-6279). Cap and invert the tube 50-60 times in
one minute. Wait 10 minutes.
5. Insert the Nitrate-N Octa-Slide 2 Bar (3109-01)into the Octa-Slide 2 Viewer (1101).
6. Insert the test tube into the Octa-Slide 2 Viewer.
7. Match sample color to a color standard. Record as ppm Nitrate-Nitrogen.
8. To convert to ppm Nitrate (NO3), multiply the test result by 4.4.
NOTE: To obtain the actual nitrate concentration, follow with Procedure B
and subtract the nitrite reading from the reading obtained in the nitrate test.
NOTE: Thoroughly clean and rinse test tubes after each use.
PROCEDURE B - NITRITE TEST
1. Fill the sample bottle (0688) with sample water.
2. Fill test tube (0106) the 2.5 mL line with water from the sample bottle.
3. Dilute to the 5.0 mL line with *Mixed Acid Reagent (V-6278). Cap and mix.
4. Use the 0.1 g spoon (0699)to add one level measure (avoid any excess) of *Color
Developing Reagent (V-6281). Cap and invert the tube 50-60 times in one minute.
Wait 10 minutes.
5. Insert the Nitrate-N Octa-Slide 2 Bar (3109-01)into the Octa-Slide 2 Viewer (1101).
6. Insert the test tube into the Octa-Slide 2 Viewer.
7. Match sample color to a color standard. Record as ppm Nitrite as Nitrate-Nitrogen.
8. Use the following table to convert reading to Nitrite-Nitrogen. Record as ppm
Nitrite-Nitrogen.
Comparator Reading Divide By
0.5 or less 3.5
0.5-1.5 4.6
over 2.0 5.8
NOTE: If the nitrate-nitrogen reading is higher than 10.0 the sample can be
diluted 1:1 with nitrate and nitrite free water, the test run and the resultant value
obtained multiplied by 2.
NOTE: Thoroughly clean and rinse test tubes after each use.
LaMOTTE COMPANY
Helping People Solve Analytical Challenges
PO Box 329 • Chestertown • Maryland • 21620 • USA
800-344-3100 • 410-778-3100 (Outside U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
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