LaMotte MO-DC User Manual

MOLYBDENUM KIT

DROP COUNT, 1 DROP = 2 ppm or 20 ppm

CODE 3632-01

QUANTITY CONTENTS CODE

120 mL *Mo Buffer *3997-J
60 mL *Denatured Alcohol *3998-H
60 mL Mo Titrant 3999-H

0.25 g Sym-Diphenylcarbazone 4001-S
1 Pipet, 1.0 mL, plastic 0354
1 Pipet, 1.0 mL, plastic, w/cap 0372
1 Pipet, plain, glass, w/cap 0371
2 Test Tubes, 2.5-5-10-15-20 mL, glass, w/caps 0970-S
1 Plug, dropper tip 28831
1 Cap 28832
1 Label, Mo Indicator 37088-4000-H

*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents go to
www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.

To order individual reagents or test kit components, use the specifi ed code number.

WARNING! This set contains chemicals

that may be harmful if misused. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision

LaMOTTE COMPANY

Helping People Solve Analytical Challenges

PO Box 329 • Chestertown • Maryland • 21620 • USA

800-344-3100 • 410-778-3100 (Outside U.S.A.) • Fax [phone]

Visit us on the web at www.lamotte.com

63632-01 5/12

®

BEFORE TESTING:

A. Replace the black cap on *Mo Buffer (3997) with the plastic pipet assembly (0372).
B. Replace the black cap on Mo Titrant (3999) with the glass pipet assembly (0371).

PREPARATION OF *MO INDICATOR (4000-H)

*Mo Indicator (4000-H) must be prepared when you receive the kit.
If stored in a cool, dark place, it can be used for about 6 months.

1. Very carefully pour all of the *Denatured Alcohol (3998) into
the bottle containing the Sym-Dyphenylcarbazone (4001). Cap
and shake gently to mix.

2. Insert the dropper plug (28831) by fi rmly pressing into the neck
of the bottle until you hear a snapping sound. Recap bottle using
cap (28832).

3. Apply the Mo Indicator label (37088-4000-H) over top of the
label on the bottle.

NOTE: Consult MSDS.

SAMPLES LESS THAN 20 ppm MOLYBDENUM

1. Fill two clean test tubes (0970-S) to the 10 mL line with sample water.

2. Add 1.0 mL *Mo Buffer (3997) to each tube. Swirl to mix.

3. Holding bottle vertically, add 10 drops of *Mo Indicator (4000) to each tube.
Swirl to mix. Sample will turn pinkish-orange.

4. Use one tube as the “reference tube” and the other as the “sample tube.”

TO THE REFERENCE TUBE

5. Fill pipet (0371) with Mo Titrant (3999). While gently swirling the tube, add
Mo Titrant (3999), one drop at a time, until pink color changes to yellow, and no
further color change occurs. Hold pipet vertically.

TO THE SAMPLE

6. Fill pipet (0371) with Mo Titrant (3999). While gently swirling the tube, add Mo
Titrant (3999), one drop at a time, until pink color changes to yellow, matching
the reference tube. Count the number of drops added. Hold pipet vertically.

7. Multiply number of drops used in Step 6 by 2. Record as ppm Molybdenum.

# of drops x 2 = ppm Molybdenum (Mo)

To convert Molybdenum (Mo) reading to Molydbate (MoO

=

), multiply by

4

1.7. Record as ppm Molybdate.

To convert Molybdenum (Mo) reading to Sodium Molybdate
(Na2MoO4 - 2H2O), multiply by 2.52. Record as ppm Sodium Molybdate.

SAMPLES GREATER THAN 20 ppm MOLYBDATE

1. Use the 1.0 mL pipet (0354) to add 1.0 mL of sample water to each test tube (0970-S).

2. Use the pipet (0372) to add 5 drops of *Mo Buffer (3997) to each tube. Swirl to mix.

3. Follow Steps 3 to 6 above.

4. Multiply number of drops used in Step 6 by 20. Record as ppm Molybdenum.

# of Drops x 20 = ppm Molybdenum (Mo)